Rh7DG025400

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000468
Physical Location & Seq
Forward (+)
1966789 .. 1967349
561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG025400.1

Sequence Viewer

Length: 561 bp
ATGAATAATGGTGTGAGAAGCCTGATGGTTCAAAGTAACCATGAAATGAATAGGGATAAGTTACGTAAGAAAAAATTAATGCATATTGTTGTATTGGTTATTGAATTTCTGATGTTGGTCATGGGTGCTCAAAGGCGACGCAAAAGGTTTATGAGAAAACAACCTTCAAGAAGTGAAGTTGATGAAAAGACATATAGAAGGATGCTTTGGATGAGAGTCTTAGAACATGAGTCTTCTTGTTTTGAACAGTTACGCATGAAACCTGCTGCATTTGAGAAGTTTTGTGCACTCTTACGGGATAGAGGGGGATTGGTGGTTACAAGAAATGTTACAATTAAAGAAGTTGTGGCATCATTTTTGCACATGCTTGGACATAATCTAAAAAATAGTACTATCAAAGCTATCTATGCTCGTTCAGAAGAAACCATAAGTAGGCAATTTCATCATGTTTTGAGATCAGTAGTCAAGCTTGGGAAGTTATACTTGAAGCAACATGAGTTAGTTCTCAATGAGAGAGACTTTGAGAAGTGGAGATGGTTTCAAGTACTAGAAATCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

186

Amino Acids

22.34

Weight (kDa)

10.4

Isoelectric Point (pI)

59.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 75 - 156 2.2e-24 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 271
AcsI RAATTY 1 cut(s) 104
AfaI GTAC 2 cut(s) 391, 546
AfiI CCNNNNNNNGG 1 cut(s) 432
AgsI TTSAA 6 cut(s) 32, 104, 168, 245, 487, 542
AjuI GAANNNNNNNTTGG 2 cut(s) 190, 222
AluBI AGCT 2 cut(s) 401, 469
AluI AGCT 2 cut(s) 401, 469
Alw21I GWGCWC 2 cut(s) 130, 289
Alw26I GTCTC 1 cut(s) 510
Alw44I GTGCAC 1 cut(s) 285
ApaLI GTGCAC 1 cut(s) 285
ApeKI GCWGC 1 cut(s) 266
ApoI RAATTY 1 cut(s) 104
AseI ATTAAT 1 cut(s) 77
BaeGI GKGCMC 1 cut(s) 289
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 130, 289
BbvI GCAGC 1 cut(s) 253
BccI CCATC 2 cut(s) 19, 528
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 1 cut(s) 548
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
BmcAI AGTACT 2 cut(s) 391, 546
BmsI GCATC 2 cut(s) 192, 359
BpiI GAAGAC 1 cut(s) 225
BsaAI YACGTR 1 cut(s) 65
Bsc4I CCNNNNNNNGG 1 cut(s) 432
BseGI GGATG 2 cut(s) 207, 216
BseLI CCNNNNNNNGG 1 cut(s) 432
BseSI GKGCMC 1 cut(s) 289
BseXI GCAGC 1 cut(s) 253
BsiHKAI GWGCWC 2 cut(s) 130, 289
BslI CCNNNNNNNGG 1 cut(s) 432
BsmAI GTCTC 1 cut(s) 510
Bsp1286I GDGCHC 2 cut(s) 130, 289
Bsp143I GATC 1 cut(s) 455
BspMI ACCTGC 1 cut(s) 271
BssMI GATC 1 cut(s) 455
Bst4CI ACNGT 1 cut(s) 249
BstBAI YACGTR 1 cut(s) 65
BstDEI CTNAG 1 cut(s) 220
BstF5I GGATG 2 cut(s) 207, 216
BstKTI GATC 1 cut(s) 458
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 1 cut(s) 455
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstNSI RCATGY 1 cut(s) 367
BstSLI GKGCMC 1 cut(s) 289
BstSNI TACGTA 1 cut(s) 65
BstV1I GCAGC 1 cut(s) 253
BstV2I GAAGAC 1 cut(s) 225
BtsCI GGATG 2 cut(s) 207, 216
BveI ACCTGC 1 cut(s) 271
CseI GACGC 1 cut(s) 147
Csp6I GTAC 2 cut(s) 390, 545
CviAII CATG 7 cut(s) 41, 121, 227, 256, 364, 446, 494
CviJI RGCY 3 cut(s) 21, 401, 469
CviKI_1 RGCY 3 cut(s) 21, 401, 469
CviQI GTAC 2 cut(s) 390, 545
DdeI CTNAG 1 cut(s) 220
DpnI GATC 1 cut(s) 457
DpnII GATC 1 cut(s) 455
Eco105I TACGTA 1 cut(s) 65
EcoT22I ATGCAT 1 cut(s) 84
FaeI CATG 7 cut(s) 44, 124, 230, 259, 367, 449, 497
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FatI CATG 7 cut(s) 40, 120, 226, 255, 363, 445, 493
Fnu4HI GCNGC 1 cut(s) 267
FokI GGATG 2 cut(s) 214, 223
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 1 cut(s) 548
GluI GCNGC 1 cut(s) 267
HgaI GACGC 1 cut(s) 147
Hin1II CATG 7 cut(s) 44, 124, 230, 259, 367, 449, 497
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 2 cut(s) 216, 230
Hpy166II GTNNAC 1 cut(s) 287
Hpy188I TCNGA 2 cut(s) 111, 418
Hpy188III TCNNGA 1 cut(s) 168
Hpy8I GTNNAC 1 cut(s) 287
Hpy99I CGWCG 1 cut(s) 141
HpyAV CCTTC 2 cut(s) 174, 192
HpyCH4III ACNGT 1 cut(s) 249
HpyCH4IV ACGT 1 cut(s) 64
HpyCH4V TGCA 4 cut(s) 82, 269, 287, 361
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 1 cut(s) 220
HpySE526I ACGT 1 cut(s) 64
Hsp92II CATG 7 cut(s) 44, 124, 230, 259, 367, 449, 497
Kzo9I GATC 1 cut(s) 455
LpnPI CCDG 2 cut(s) 35, 276
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 2 cut(s) 192, 359
MaeI CTAG 1 cut(s) 548
MaeII ACGT 1 cut(s) 64
MaeIII GTNAC 5 cut(s) 35, 60, 249, 316, 328
MalI GATC 1 cut(s) 457
MboI GATC 1 cut(s) 455
MboII GAAGA 2 cut(s) 225, 431
MhlI GDGCHC 2 cut(s) 130, 289
MluCI AATT 4 cut(s) 74, 104, 333, 437
MlyI GAGTC 2 cut(s) 225, 239
MnlI CCTC 1 cut(s) 296
Mph1103I ATGCAT 1 cut(s) 84
MseI TTAA 2 cut(s) 77, 336
MwoI GCNNNNNNNGC 1 cut(s) 407
NdeII GATC 1 cut(s) 455
NlaIII CATG 7 cut(s) 44, 124, 230, 259, 367, 449, 497
NsiI ATGCAT 1 cut(s) 84
NspI RCATGY 1 cut(s) 367
PkrI GCNGC 1 cut(s) 268
PleI GAGTC 2 cut(s) 224, 238
PpsI GAGTC 2 cut(s) 224, 238
Ppu21I YACGTR 1 cut(s) 65
PshBI ATTAAT 1 cut(s) 77
RsaI GTAC 2 cut(s) 391, 546
RsaNI GTAC 2 cut(s) 390, 545
SaqAI TTAA 2 cut(s) 77, 336
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 1 cut(s) 455
ScaI AGTACT 2 cut(s) 391, 546
SchI GAGTC 2 cut(s) 225, 239
SduI GDGCHC 2 cut(s) 130, 289
SetI ASST 6 cut(s) 67, 149, 166, 265, 403, 471
SfaNI GCATC 2 cut(s) 192, 359
SnaBI TACGTA 1 cut(s) 65
Sse9I AATT 4 cut(s) 74, 104, 333, 437
SspMI CTAG 1 cut(s) 548
TaaI ACNGT 1 cut(s) 249
TaiI ACGT 1 cut(s) 67
TasI AATT 4 cut(s) 74, 104, 333, 437
TatI WGTACW 2 cut(s) 389, 544
Tru1I TTAA 2 cut(s) 77, 336
Tru9I TTAA 2 cut(s) 77, 336
TseI GCWGC 1 cut(s) 266
TspDTI ATGAA 6 cut(s) 17, 57, 62, 198, 272, 431
VneI GTGCAC 1 cut(s) 285
VspI ATTAAT 1 cut(s) 77
XapI RAATTY 1 cut(s) 104
XceI RCATGY 1 cut(s) 367
XspI CTAG 1 cut(s) 548
ZrmI AGTACT 2 cut(s) 391, 546
Zsp2I ATGCAT 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.