pycom15g16780
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000468
Physical Location & Seq
Forward (+)
11777149 .. 11779835
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g16780.5

Sequence Viewer

Length: 771 bp
ATGGCCCTGTTTGGCACACCGTATAAGGTACCGGATAGGACTAATAATACGGTGTACTACCGTGCAAATGCACGGTATAATTTAGTCGGGCTCTCTGAAAGACCAGTCTTCCGCGCCCTCTCTTCCTCTCCAGGAGCTCTCCAGGCGCTCTCTCTTCCTCTTCAGGTGCTCTCTCTTCCTCTCATCCCAGACATGGATAAAAGAAAGATTGTGGCATGCTTCATTGTTAACGTGTGGTACTTGAATATTGTGACTTGGTTGATGATGTGGTTCATCAAGAATCATAATTCAGCTAGAAGGCGAAATTGGCAACTTGCACTTAGAAATCGCACATTTGTTAGGAGAATGGTAGGGTCAACCTATTTAGATAGCATAATAGGATATGATGATGTTCAGTGTGTGAACCAATTTAGAATGGTTAGAAGAACGTTCCATTATTTATGTCAAATGCTTCGTGACCACGGTAAAGTAAAATTGGATGGTGTAGTGACAATGGAAGAACAAGTTTGCATGTTTTTGCATATACTTGCACATCATGTCAAGAATCGTACAATTGGGAGTAGGTTTTGTCGATCAAGGGAGACCATAAGTAGGTATTTCAATTCCATTCTACATGGAGTGTTAAGGCTACAAGGTAAACTTTTGAAAGTGCCAGAACCCATTCCTAATGGAGTGCATATGATTGACAGTGTTCAACCTACACAAGAATGGACTGCATGGAGAAACACTTTGGCTATGAATATGTACAATGAGCGGCGTGCACAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

257

Amino Acids

29.99

Weight (kDa)

10.31

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 28
AccBSI CCGCTC 1 cut(s) 754
AccII CGCG 1 cut(s) 114
AciI CCGC 2 cut(s) 112, 754
AclI AACGTT 1 cut(s) 428
AcuI CTGAAG 1 cut(s) 146
AfaI GTAC 5 cut(s) 30, 56, 239, 550, 746
AfiI CCNNNNNNNGG 2 cut(s) 193, 591
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 4 cut(s) 244, 601, 646, 695
AjnI CCWGG 2 cut(s) 130, 141
AjuI GAANNNNNNNTTGG 2 cut(s) 289, 321
AleI CACNNNNGTG 1 cut(s) 766
AluBI AGCT 2 cut(s) 137, 293
AluI AGCT 2 cut(s) 137, 293
Alw21I GWGCWC 3 cut(s) 139, 171, 763
Alw26I GTCTC 1 cut(s) 575
Alw44I GTGCAC 1 cut(s) 759
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 660
Asp718I GGTACC 1 cut(s) 28
AspLEI GCGC 2 cut(s) 116, 148
AspS9I GGNCC 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 763
BanI GGYRCC 1 cut(s) 28
BanII GRGCYC 2 cut(s) 93, 139
BbsI GAAGAC 1 cut(s) 100
Bbv12I GWGCWC 3 cut(s) 139, 171, 763
BccI CCATC 1 cut(s) 473
BciT130I CCWGG 2 cut(s) 132, 143
BcoDI GTCTC 1 cut(s) 575
BfaI CTAG 1 cut(s) 294
BfoI RGCGCY 1 cut(s) 149
BisI GCNGC 1 cut(s) 755
BlsI GCNGC 1 cut(s) 756
Bme1390I CCNGG 2 cut(s) 132, 143
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 132, 143
BpiI GAAGAC 1 cut(s) 100
BpmI CTGGAG 2 cut(s) 114, 125
BsaI GGTCTC 1 cut(s) 575
BsaJI CCNNGG 1 cut(s) 460
BsaWI WCCGGW 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 591
Bse1I ACTGG 1 cut(s) 104
BseBI CCWGG 2 cut(s) 132, 143
BseDI CCNNGG 1 cut(s) 460
BseGI GGATG 2 cut(s) 183, 484
BseLI CCNNNNNNNGG 2 cut(s) 193, 591
BseNI ACTGG 1 cut(s) 104
BseSI GKGCMC 1 cut(s) 763
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 28
BsiHKAI GWGCWC 3 cut(s) 139, 171, 763
BsiSI CCGG 1 cut(s) 32
BslI CCNNNNNNNGG 2 cut(s) 193, 591
BsmAI GTCTC 1 cut(s) 575
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 575
Bsp1286I GDGCHC 4 cut(s) 93, 139, 171, 763
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 1 cut(s) 572
BspACI CCGC 2 cut(s) 112, 754
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 28
BspTNI GGTCTC 1 cut(s) 575
BsrBI CCGCTC 1 cut(s) 754
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 460
BssMI GATC 1 cut(s) 572
Bst2UI CCWGG 2 cut(s) 132, 143
Bst4CI ACNGT 6 cut(s) 21, 52, 62, 75, 464, 689
Bst6I CTCTTC 4 cut(s) 127, 159, 165, 180
BstAUI TGTACA 1 cut(s) 744
BstC8I GCNNGC 2 cut(s) 217, 759
BstDEI CTNAG 1 cut(s) 320
BstDSI CCRYGG 1 cut(s) 460
BstF5I GGATG 2 cut(s) 183, 484
BstFNI CGCG 1 cut(s) 114
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 2 cut(s) 116, 148
BstKTI GATC 1 cut(s) 575
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 1 cut(s) 572
BstMWI GCNNNNNNNGC 2 cut(s) 143, 307
BstNI CCWGG 2 cut(s) 132, 143
BstNSI RCATGY 2 cut(s) 219, 514
BstSCI CCNGG 2 cut(s) 130, 141
BstSLI GKGCMC 1 cut(s) 763
BstUI CGCG 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 460
BtsCI GGATG 2 cut(s) 183, 484
BtsIMutI CAGTG 2 cut(s) 401, 694
Cac8I GCNNGC 2 cut(s) 217, 759
CfoI GCGC 2 cut(s) 116, 148
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 5 cut(s) 29, 55, 238, 549, 745
CviAII CATG 6 cut(s) 193, 216, 511, 536, 614, 717
CviJI RGCY 6 cut(s) 5, 91, 137, 293, 628, 734
CviKI_1 RGCY 6 cut(s) 5, 91, 137, 293, 628, 734
CviQI GTAC 5 cut(s) 29, 55, 238, 549, 745
DdeI CTNAG 1 cut(s) 320
DpnI GATC 1 cut(s) 574
DpnII GATC 1 cut(s) 572
Eam1104I CTCTTC 4 cut(s) 127, 159, 165, 180
EarI CTCTTC 4 cut(s) 127, 159, 165, 180
Ecl136II GAGCTC 1 cut(s) 137
Eco24I GRGCYC 2 cut(s) 93, 139
Eco31I GGTCTC 1 cut(s) 575
Eco53kI GAGCTC 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 137
EcoRII CCWGG 2 cut(s) 130, 141
EcoT38I GRGCYC 2 cut(s) 93, 139
FaeI CATG 6 cut(s) 196, 219, 514, 539, 617, 720
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FatI CATG 6 cut(s) 192, 215, 510, 535, 613, 716
FauNDI CATATG 1 cut(s) 678
Fnu4HI GCNGC 1 cut(s) 755
FokI GGATG 2 cut(s) 170, 491
FriOI GRGCYC 2 cut(s) 93, 139
Fsp4HI GCNGC 1 cut(s) 755
FspBI CTAG 1 cut(s) 294
GlaI GCGC 2 cut(s) 115, 147
GluI GCNGC 1 cut(s) 755
GsuI CTGGAG 2 cut(s) 114, 125
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 32
HhaI GCGC 2 cut(s) 116, 148
Hin1II CATG 6 cut(s) 196, 219, 514, 539, 617, 720
Hin6I GCGC 2 cut(s) 114, 146
HinP1I GCGC 2 cut(s) 114, 146
HincII GTYRAC 2 cut(s) 229, 357
HindII GTYRAC 2 cut(s) 229, 357
HinfI GANTC 2 cut(s) 280, 544
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 1 cut(s) 32
Hpy166II GTNNAC 6 cut(s) 55, 229, 357, 403, 638, 761
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 3 cut(s) 277, 455, 541
Hpy8I GTNNAC 6 cut(s) 55, 229, 357, 403, 638, 761
HpyAV CCTTC 1 cut(s) 291
HpyCH4III ACNGT 6 cut(s) 21, 52, 62, 75, 464, 689
HpyCH4IV ACGT 2 cut(s) 231, 428
HpyCH4V TGCA 9 cut(s) 65, 71, 317, 510, 520, 530, 676, 716, 761
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 307
HpyF3I CTNAG 1 cut(s) 320
HpySE526I ACGT 2 cut(s) 231, 428
Hsp92II CATG 6 cut(s) 196, 219, 514, 539, 617, 720
HspAI GCGC 2 cut(s) 114, 146
KpnI GGTACC 1 cut(s) 32
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 1 cut(s) 572
LmnI GCTCC 1 cut(s) 134
MaeI CTAG 1 cut(s) 294
MaeII ACGT 2 cut(s) 231, 428
MaeIII GTNAC 3 cut(s) 250, 455, 487
MalI GATC 1 cut(s) 574
MbiI CCGCTC 1 cut(s) 754
MboI GATC 1 cut(s) 572
MboII GAAGA 7 cut(s) 100, 114, 146, 152, 167, 435, 509
MfeI CAATTG 1 cut(s) 552
MhlI GDGCHC 4 cut(s) 93, 139, 171, 763
MluCI AATT 7 cut(s) 79, 286, 304, 407, 473, 552, 601
MnlI CCTC 4 cut(s) 128, 136, 168, 189
MroXI GAANNNNTTC 1 cut(s) 660
MseI TTAA 2 cut(s) 228, 623
MslI CAYNNNNRTG 2 cut(s) 706, 766
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 132, 143
MunI CAATTG 1 cut(s) 552
MvaI CCWGG 2 cut(s) 132, 143
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 2 cut(s) 143, 307
NdeI CATATG 1 cut(s) 678
NdeII GATC 1 cut(s) 572
NlaIII CATG 6 cut(s) 196, 219, 514, 539, 617, 720
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 3 cut(s) 250, 455, 487
NspI RCATGY 2 cut(s) 219, 514
OliI CACNNNNGTG 1 cut(s) 766
PaeI GCATGC 1 cut(s) 219
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 2 cut(s) 280, 544
PfoI TCCNGGA 1 cut(s) 130
PkrI GCNGC 1 cut(s) 756
Psp124BI GAGCTC 1 cut(s) 139
Psp1406I AACGTT 1 cut(s) 428
Psp6I CCWGG 2 cut(s) 130, 141
PspGI CCWGG 2 cut(s) 130, 141
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 4
RsaI GTAC 5 cut(s) 30, 56, 239, 550, 746
RsaNI GTAC 5 cut(s) 29, 55, 238, 549, 745
RseI CAYNNNNRTG 2 cut(s) 706, 766
SacI GAGCTC 1 cut(s) 139
SaqAI TTAA 2 cut(s) 228, 623
SatI GCNGC 1 cut(s) 755
Sau3AI GATC 1 cut(s) 572
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 2 cut(s) 132, 143
SduI GDGCHC 4 cut(s) 93, 139, 171, 763
SmiMI CAYNNNNRTG 2 cut(s) 706, 766
SphI GCATGC 1 cut(s) 219
Sse9I AATT 7 cut(s) 79, 286, 304, 407, 473, 552, 601
SsiI CCGC 2 cut(s) 112, 754
SspI AATATT 1 cut(s) 247
SspMI CTAG 1 cut(s) 294
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 2 cut(s) 130, 141
TaaI ACNGT 6 cut(s) 21, 52, 62, 75, 464, 689
TaiI ACGT 2 cut(s) 234, 431
TaqI TCGA 1 cut(s) 571
TasI AATT 7 cut(s) 79, 286, 304, 407, 473, 552, 601
TatI WGTACW 2 cut(s) 54, 744
TauI GCSGC 1 cut(s) 757
TfiI GAWTC 2 cut(s) 280, 544
Tru1I TTAA 2 cut(s) 228, 623
Tru9I TTAA 2 cut(s) 228, 623
TscAI CASTG 2 cut(s) 401, 694
TseFI GTSAC 3 cut(s) 250, 455, 487
Tsp45I GTSAC 3 cut(s) 250, 455, 487
TspDTI ATGAA 3 cut(s) 211, 262, 752
TspRI CASTG 2 cut(s) 401, 694
VneI GTGCAC 1 cut(s) 759
XceI RCATGY 2 cut(s) 219, 514
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.