Rw7G014310
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000468
Physical Location & Seq
Reverse (-)
13903810 .. 13905317
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G014310.1

Sequence Viewer

Length: 1272 bp
ATGGATCGAAGGCAAATTATGTTGCTCTTATTGGTGGAGCTGTGGCACCTAGAGACAATGTGTGCTTGTACTATGCTTGTGCTATTGATGTTTAATTTGAGACGAAGACGTGCTGAAAGACATGCTCTAAATAATCGTTCATTCGTTAGACGTGAGACTCGTTTGAGTTATCTAGATAGTATTATAGGCAATAGTGACACCGAATGTGTACATGAATTAAGAATGGATAGAAGGACTTTTGGCCTTTTGTGTGAACTGCTTCGCACAGATGGAAAGATAAAGAGTGATGGTTTGGTGTCTGTGGAAGAGCAAGTATGTATGTTTTTACATATACTTTCACATCATGTGAAGAATCGTACTATTAGAGGTAGATTTTTTCGATCCGGAGAGACTATTAGTAGGTATTTCCATTCTATATTACAAGTACCTGATCCGGTACTTGCTGACTGCACAGATCGTAGATGGAAGTGCTTTAAGAATTGCTTGGGAGCACTAGATGGAACTTACATTAGAGTGCGTGTACCTGCAATTGACAAGCCAAGATACCGCACAAGAAAGGGTGAAGTTGCTACAAATGTGTTAGCTGCTTGTTCTCGTGATATGCAGTTCATATTTGTCTTACCGGGTTGGGAGGGATCGGCATCAGACTCTAGAGTTCTTCACGATGCAGTGACTAGGCCGAATGGATTAAGAGTTCCCACTGGTTATTATTACCTTGTAGATGGTGGTTACACAAATGGTGAGGGATTTCTTGCACCATATAGAGGAACAAGGTATCATTTGTCGGAATGGGATGAAAGAAATATGCCTAGGGATCATGTGGAATATTTTAACATGAAGCATTCGAAGGCAAGGAATGTCATAGAACGGTGTTTTGGAATACTAAAAGGAAGGTGGGGAATACTAAGGAGTCCATCATTTTATCCAATAAAGACACAATGCCGCATAATTACGGTTTGTTGTCTTTTACATAATTTTATTAGGAGAGAGATGTCCATTGATCCAATGGAGTATGCAGTATGTGAATTAGATGCACAAAATGGGGAAGAAGTTGATGTAGTTGGCACGGTTGAAGCATCTAATCAATGGACTACTTGGAGGAGTGAATTAGCTTTACAAATGCATAATGAATGGACGGGAAGAAGGGCTTACCGGGATGCTGGAGATACAGGGGTTTCAGGTGCTGCTGCAAGGGCTGCAAGGGATGCAAGAGCTGCAAGGGATGCAAGAGCTGGAAGGGACGGAAGAGCTGCAAGGGACGGAAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

423

Amino Acids

48.76

Weight (kDa)

9.33

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 56 - 141 9.8e-24 Domain of unknown function (DUF8040)
DDE_Tnp_1 PF01609 161 - 323 3.5e-10 Transposase DDE domain
DDE_Tnp_4 PF13359 165 - 325 7.6e-28 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 532
AccB1I GGYRCC 1 cut(s) 45
AccIII TCCGGA 1 cut(s) 383
AciI CCGC 2 cut(s) 547, 943
AclWI GGATC 6 cut(s) 12, 375, 425, 643, 822, 995
AfaI GTAC 6 cut(s) 70, 210, 358, 426, 438, 522
AfiI CCNNNNNNNGG 1 cut(s) 764
AgsI TTSAA 1 cut(s) 1073
AjiI CACGTC 2 cut(s) 110, 152
AjuI GAANNNNNNNTTGG 2 cut(s) 858, 890
AloI GAACNNNNNNTCC 2 cut(s) 678, 710
AluBI AGCT 6 cut(s) 40, 584, 1112, 1214, 1232, 1250
AluI AGCT 6 cut(s) 40, 584, 1112, 1214, 1232, 1250
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 4 cut(s) 47, 94, 149, 383
AlwI GGATC 6 cut(s) 12, 375, 425, 643, 822, 995
AlwNI CAGNNNCTG 1 cut(s) 1184
Aor13HI TCCGGA 1 cut(s) 383
AoxI GGCC 2 cut(s) 241, 677
ApeKI GCWGC 6 cut(s) 584, 1184, 1187, 1196, 1214, 1250
Asp700I GAANNNNTTC 1 cut(s) 258
AspA2I CCTAGG 1 cut(s) 809
AsuC2I CCSGG 2 cut(s) 624, 1154
AsuHPI GGTGA 2 cut(s) 572, 752
AsuII TTCGAA 1 cut(s) 845
AvrII CCTAGG 1 cut(s) 809
BaeI ACNNNNGTAYC 2 cut(s) 1158, 1191
BanI GGYRCC 1 cut(s) 45
BauI CACGAG 1 cut(s) 594
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 6 cut(s) 571, 1171, 1174, 1183, 1201, 1237
BccI CCATC 6 cut(s) 263, 281, 456, 491, 716, 922
BcnI CCSGG 2 cut(s) 624, 1154
BcoDI GTCTC 4 cut(s) 47, 94, 149, 383
BfaI CTAG 6 cut(s) 50, 173, 494, 651, 675, 810
BfuAI ACCTGC 1 cut(s) 532
BisI GCNGC 7 cut(s) 585, 943, 1185, 1188, 1197, 1215, 1251
BlnI CCTAGG 1 cut(s) 809
BlsI GCNGC 7 cut(s) 586, 944, 1186, 1189, 1198, 1216, 1252
Bme1390I CCNGG 2 cut(s) 624, 1154
BmgBI CACGTC 2 cut(s) 110, 152
BmiI GGNNCC 1 cut(s) 47
BmrFI CCNGG 2 cut(s) 624, 1154
BmsI GCATC 7 cut(s) 650, 655, 1021, 1085, 1147, 1195, 1213
BpiI GAAGAC 1 cut(s) 112
BpmI CTGGAG 1 cut(s) 1182
Bpu14I TTCGAA 1 cut(s) 845
BpuMI CCSGG 2 cut(s) 624, 1154
BsaBI GATNNNNATC 1 cut(s) 640
BsaJI CCNNGG 1 cut(s) 809
BsaWI WCCGGW 2 cut(s) 383, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 764
Bse1I ACTGG 1 cut(s) 706
Bse8I GATNNNNATC 1 cut(s) 640
BseAI TCCGGA 1 cut(s) 383
BseDI CCNNGG 1 cut(s) 809
BseGI GGATG 4 cut(s) 799, 1162, 1210, 1228
BseJI GATNNNNATC 1 cut(s) 640
BseLI CCNNNNNNNGG 1 cut(s) 764
BseNI ACTGG 1 cut(s) 706
BseRI GAGGAG 1 cut(s) 1114
BseXI GCAGC 6 cut(s) 571, 1171, 1174, 1183, 1201, 1237
BsgI GTGCAG 1 cut(s) 433
BshFI GGCC 2 cut(s) 243, 679
BshNI GGYRCC 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 4 cut(s) 384, 434, 623, 1153
BslFI GGGAC 1 cut(s) 1253
BslI CCNNNNNNNGG 1 cut(s) 764
BsmAI GTCTC 4 cut(s) 47, 94, 149, 383
BsmBI CGTCTC 1 cut(s) 94
BsmFI GGGAC 1 cut(s) 1253
BsmI GAATGC 1 cut(s) 841
BsnI GGCC 2 cut(s) 243, 679
Bsp119I TTCGAA 1 cut(s) 845
Bsp1286I GDGCHC 1 cut(s) 493
Bsp13I TCCGGA 1 cut(s) 383
Bsp1407I TGTACA 1 cut(s) 208
Bsp143I GATC 7 cut(s) 4, 380, 430, 454, 635, 814, 1000
BspACI CCGC 2 cut(s) 547, 943
BspANI GGCC 2 cut(s) 243, 679
BspEI TCCGGA 1 cut(s) 383
BspLI GGNNCC 1 cut(s) 47
BspMI ACCTGC 1 cut(s) 532
BspPI GGATC 6 cut(s) 12, 375, 425, 643, 822, 995
BspQI GCTCTTC 2 cut(s) 300, 1240
BspT104I TTCGAA 1 cut(s) 845
BspT107I GGYRCC 1 cut(s) 45
BsrGI TGTACA 1 cut(s) 208
BsrI ACTGG 1 cut(s) 706
BssECI CCNNGG 1 cut(s) 809
BssMI GATC 7 cut(s) 4, 380, 430, 454, 635, 814, 1000
BssSI CACGAG 1 cut(s) 594
BssT1I CCWWGG 1 cut(s) 809
Bst2BI CACGAG 1 cut(s) 594
Bst4CI ACNGT 3 cut(s) 870, 955, 1069
Bst6I CTCTTC 3 cut(s) 300, 1240, 1258
BstAPI GCANNNNNTGC 4 cut(s) 1196, 1205, 1214, 1223
BstAUI TGTACA 1 cut(s) 208
BstBI TTCGAA 1 cut(s) 845
BstDEI CTNAG 1 cut(s) 905
BstF5I GGATG 4 cut(s) 799, 1162, 1210, 1228
BstKTI GATC 7 cut(s) 7, 383, 433, 457, 638, 817, 1003
BstMAI GTCTC 4 cut(s) 47, 94, 149, 383
BstMBI GATC 7 cut(s) 4, 380, 430, 454, 635, 814, 1000
BstMWI GCNNNNNNNGC 5 cut(s) 1193, 1196, 1205, 1214, 1223
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 2 cut(s) 622, 1152
BstV1I GCAGC 6 cut(s) 571, 1171, 1174, 1183, 1201, 1237
BstV2I GAAGAC 1 cut(s) 112
BsuRI GGCC 2 cut(s) 243, 679
BtrI CACGTC 2 cut(s) 110, 152
BtsCI GGATG 4 cut(s) 799, 1162, 1210, 1228
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 2 cut(s) 675, 699
BveI ACCTGC 1 cut(s) 532
CaiI CAGNNNCTG 1 cut(s) 1184
Csp6I GTAC 6 cut(s) 69, 209, 357, 425, 437, 521
CviAII CATG 5 cut(s) 122, 212, 344, 818, 835
CviQI GTAC 6 cut(s) 69, 209, 357, 425, 437, 521
DdeI CTNAG 1 cut(s) 905
DpnI GATC 7 cut(s) 6, 382, 432, 456, 637, 816, 1002
DpnII GATC 7 cut(s) 4, 380, 430, 454, 635, 814, 1000
Eam1104I CTCTTC 3 cut(s) 300, 1240, 1258
EarI CTCTTC 3 cut(s) 300, 1240, 1258
Eco130I CCWWGG 1 cut(s) 809
EcoT14I CCWWGG 1 cut(s) 809
EcoT22I ATGCAT 1 cut(s) 1125
ErhI CCWWGG 1 cut(s) 809
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 5 cut(s) 125, 215, 347, 821, 838
FalI AAGNNNNNCTT 4 cut(s) 467, 499, 1132, 1164
FaqI GGGAC 1 cut(s) 1253
FatI CATG 5 cut(s) 121, 211, 343, 817, 834
Fnu4HI GCNGC 7 cut(s) 585, 943, 1185, 1188, 1197, 1215, 1251
FokI GGATG 4 cut(s) 806, 1169, 1217, 1235
Fsp4HI GCNGC 7 cut(s) 585, 943, 1185, 1188, 1197, 1215, 1251
FspBI CTAG 6 cut(s) 50, 173, 494, 651, 675, 810
GluI GCNGC 7 cut(s) 585, 943, 1185, 1188, 1197, 1215, 1251
GsuI CTGGAG 1 cut(s) 1182
HaeIII GGCC 2 cut(s) 243, 679
HapII CCGG 4 cut(s) 384, 434, 623, 1153
Hin1II CATG 5 cut(s) 125, 215, 347, 821, 838
HinfI GANTC 4 cut(s) 157, 352, 647, 910
HpaII CCGG 4 cut(s) 384, 434, 623, 1153
HphI GGTGA 2 cut(s) 572, 752
Hpy166II GTNNAC 3 cut(s) 209, 254, 521
Hpy188I TCNGA 2 cut(s) 646, 787
Hpy188III TCNNGA 5 cut(s) 173, 384, 596, 651, 662
Hpy8I GTNNAC 3 cut(s) 209, 254, 521
HpyAV CCTTC 6 cut(s) 3, 225, 841, 885, 1137, 1230
HpyCH4III ACNGT 3 cut(s) 870, 955, 1069
HpyCH4IV ACGT 2 cut(s) 109, 151
HpyF10VI GCNNNNNNNGC 5 cut(s) 1193, 1196, 1205, 1214, 1223
HpyF3I CTNAG 1 cut(s) 905
HpySE526I ACGT 2 cut(s) 109, 151
Hsp92II CATG 5 cut(s) 125, 215, 347, 821, 838
Kpn2I TCCGGA 1 cut(s) 383
Kzo9I GATC 7 cut(s) 4, 380, 430, 454, 635, 814, 1000
LguI GCTCTTC 2 cut(s) 300, 1240
LmnI GCTCC 2 cut(s) 37, 488
Lsp1109I GCAGC 6 cut(s) 571, 1171, 1174, 1183, 1201, 1237
LweI GCATC 7 cut(s) 650, 655, 1021, 1085, 1147, 1195, 1213
MaeI CTAG 6 cut(s) 50, 173, 494, 651, 675, 810
MaeII ACGT 2 cut(s) 109, 151
MaeIII GTNAC 3 cut(s) 194, 670, 728
MalI GATC 7 cut(s) 6, 382, 432, 456, 637, 816, 1002
MboI GATC 7 cut(s) 4, 380, 430, 454, 635, 814, 1000
MboII GAAGA 7 cut(s) 117, 317, 361, 650, 1058, 1152, 1257
MfeI CAATTG 1 cut(s) 528
MhlI GDGCHC 1 cut(s) 493
MluCI AATT 9 cut(s) 15, 94, 215, 478, 528, 948, 973, 1025, 1106
MlyI GAGTC 3 cut(s) 151, 641, 919
MmeI TCCRAC 1 cut(s) 765
MnlI CCTC 5 cut(s) 359, 625, 736, 758, 1092
Mph1103I ATGCAT 1 cut(s) 1125
MroI TCCGGA 1 cut(s) 383
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 5 cut(s) 93, 218, 474, 689, 831
MslI CAYNNNNRTG 1 cut(s) 512
MspI CCGG 4 cut(s) 384, 434, 623, 1153
MspR9I CCNGG 2 cut(s) 624, 1154
MunI CAATTG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 841
MwoI GCNNNNNNNGC 5 cut(s) 1193, 1196, 1205, 1214, 1223
NciI CCSGG 2 cut(s) 624, 1154
NdeII GATC 7 cut(s) 4, 380, 430, 454, 635, 814, 1000
NlaIII CATG 5 cut(s) 125, 215, 347, 821, 838
NlaIV GGNNCC 1 cut(s) 47
NmuCI GTSAC 2 cut(s) 194, 670
NsiI ATGCAT 1 cut(s) 1125
NspI RCATGY 1 cut(s) 125
NspV TTCGAA 1 cut(s) 845
PciSI GCTCTTC 2 cut(s) 300, 1240
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 1 cut(s) 841
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 1 cut(s) 352
PkrI GCNGC 7 cut(s) 586, 944, 1186, 1189, 1198, 1216, 1252
PleI GAGTC 3 cut(s) 151, 641, 918
PpsI GAGTC 3 cut(s) 151, 641, 918
PspN4I GGNNCC 1 cut(s) 47
PstNI CAGNNNCTG 1 cut(s) 1184
RsaI GTAC 6 cut(s) 70, 210, 358, 426, 438, 522
RsaNI GTAC 6 cut(s) 69, 209, 357, 425, 437, 521
RseI CAYNNNNRTG 1 cut(s) 512
SapI GCTCTTC 2 cut(s) 300, 1240
SaqAI TTAA 5 cut(s) 93, 218, 474, 689, 831
SatI GCNGC 7 cut(s) 585, 943, 1185, 1188, 1197, 1215, 1251
Sau3AI GATC 7 cut(s) 4, 380, 430, 454, 635, 814, 1000
SchI GAGTC 3 cut(s) 151, 641, 919
ScrFI CCNGG 2 cut(s) 624, 1154
SduI GDGCHC 1 cut(s) 493
SfaNI GCATC 7 cut(s) 650, 655, 1021, 1085, 1147, 1195, 1213
SfuI TTCGAA 1 cut(s) 845
SmiMI CAYNNNNRTG 1 cut(s) 512
Sse9I AATT 9 cut(s) 15, 94, 215, 478, 528, 948, 973, 1025, 1106
SsiI CCGC 2 cut(s) 547, 943
SspI AATATT 1 cut(s) 827
SspMI CTAG 6 cut(s) 50, 173, 494, 651, 675, 810
StyD4I CCNGG 2 cut(s) 622, 1152
StyI CCWWGG 1 cut(s) 809
TaaI ACNGT 3 cut(s) 870, 955, 1069
TaiI ACGT 2 cut(s) 112, 154
TaqI TCGA 3 cut(s) 7, 379, 845
TasI AATT 9 cut(s) 15, 94, 215, 478, 528, 948, 973, 1025, 1106
TatI WGTACW 2 cut(s) 68, 208
TauI GCSGC 1 cut(s) 945
TfiI GAWTC 1 cut(s) 352
Tru1I TTAA 5 cut(s) 93, 218, 474, 689, 831
Tru9I TTAA 5 cut(s) 93, 218, 474, 689, 831
TscAI CASTG 2 cut(s) 675, 706
TseFI GTSAC 2 cut(s) 194, 670
TseI GCWGC 6 cut(s) 584, 1184, 1187, 1196, 1214, 1250
Tsp45I GTSAC 2 cut(s) 194, 670
TspDTI ATGAA 6 cut(s) 129, 228, 598, 810, 851, 1143
TspGWI ACGGA 1 cut(s) 1257
TspRI CASTG 2 cut(s) 675, 706
XbaI TCTAGA 2 cut(s) 172, 650
XceI RCATGY 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 1 cut(s) 1003
XmaJI CCTAGG 1 cut(s) 809
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 6 cut(s) 50, 173, 494, 651, 675, 810
Zsp2I ATGCAT 1 cut(s) 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.