Rw6G035650
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000468
Physical Location & Seq
Reverse (-)
60259425 .. 60260494
1070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035650.1

Sequence Viewer

Length: 843 bp
ATGGTGGCTATGTTCTTGCATATTCTTGCGCACCATGAAAATAACAGAATCATACAAGATAGATTTTTGCGTTATGGGGAAACAATTAGCAAATATTTTAACGCGGTATTAAGAGCAGTCCTATGTCTACAAGGACAATTGTTAAAAACACCAGAACCTGTTCCAGAAAACTCATCTGATGAGAAGTGGAAATGGTGTAAGAATTGTCTCGGAGCGCTAGATGGGACATACATTAGAGTACATGTGCCAGAAGCGGACAAGCCTAGGTACCGAAGTAGGAAGAATGAAATTGCTACTAATGTCTTGGGAGTATGCTCCCAAGATATGCAATTCATTTATGTATTACCGGGTTGGGAGGGTTCGGCTGCAGATTCACGAGTACTACGATATTACTACCTTGTTGATGTTGGGTATCCAAATGGGGAGAGGTTTCTTGCTCCATATAGAGGCCAGAGATATCATTTAAATGATTGGAGAGAAGGATACCAACCTACCACCCCGAATAATTTTTTTAATAAGAAGCATTCTTCTGCTAGGAATGTGATAGAGAGATGTTTTGGGTTACTTAAGATGCGATGGGCAATTCTAAGGAGCCCTTCATTTTTTCCAGTGAAGACGCAGTGCCGAATTGTTATAGCATGTTGCTTGCTTCACAACCTTATAAGAAGGGAAATGGCAGTTGATCCCTTAGAAAATGAACTGAATGCCATTGTGAATAACCAAGATGATAATGATGATGATATAATCAATTTTATCGACTCATCTAAACAATGGAATAATTGGATAAGAGAGTTGGCAACACAAATGTTCAATGAATGGAGAGGAAATCGGAACACAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

280

Amino Acids

32.92

Weight (kDa)

8.86

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 2 - 41 4.2e-11 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 73 - 219 1.7e-22 DDE superfamily endonuclease
Plant_tran PF04827 166 - 221 3e-06 Plant transposon protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 662
Acc16I TGCGCA 1 cut(s) 30
Acc65I GGTACC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 127
AccII CGCG 1 cut(s) 104
AciI CCGC 2 cut(s) 104, 254
AclWI GGATC 1 cut(s) 677
AfaI GTAC 3 cut(s) 240, 269, 381
AfeI AGCGCT 1 cut(s) 216
AfiI CCNNNNNNNGG 1 cut(s) 446
AflII CTTAAG 1 cut(s) 566
AflIII ACRYGT 1 cut(s) 241
AgsI TTSAA 1 cut(s) 811
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 1 cut(s) 677
AlwNI CAGNNNCTG 1 cut(s) 158
Aor51HI AGCGCT 1 cut(s) 216
AoxI GGCC 1 cut(s) 448
ApeKI GCWGC 1 cut(s) 365
Asp700I GAANNNNTTC 1 cut(s) 159
Asp718I GGTACC 1 cut(s) 267
AspA2I CCTAGG 1 cut(s) 263
AspLEI GCGC 2 cut(s) 31, 217
AsuC2I CCSGG 1 cut(s) 348
AvrII CCTAGG 1 cut(s) 263
BaeI ACNNNNGTAYC 2 cut(s) 259, 292
BanI GGYRCC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 596
BauI CACGAG 1 cut(s) 375
BbsI GAAGAC 1 cut(s) 620
BbvI GCAGC 1 cut(s) 352
BccI CCATC 2 cut(s) 215, 570
BciVI GTATCC 2 cut(s) 423, 476
BcnI CCSGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 3 cut(s) 218, 264, 534
BfmI CTRYAG 1 cut(s) 366
BfoI RGCGCY 1 cut(s) 218
BfrI CTTAAG 1 cut(s) 566
BfuI GTATCC 2 cut(s) 423, 476
BisI GCNGC 1 cut(s) 366
BlnI CCTAGG 1 cut(s) 263
BlsI GCNGC 1 cut(s) 367
BmcAI AGTACT 1 cut(s) 381
Bme1390I CCNGG 1 cut(s) 348
BmiI GGNNCC 2 cut(s) 269, 593
BmrFI CCNGG 1 cut(s) 348
BmsI GCATC 1 cut(s) 561
BpiI GAAGAC 1 cut(s) 620
BpuMI CCSGG 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 263
Bsc4I CCNNNNNNNGG 1 cut(s) 446
Bse1I ACTGG 1 cut(s) 608
BseDI CCNNGG 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 446
BseNI ACTGG 1 cut(s) 608
BseXI GCAGC 1 cut(s) 352
Bsh1236I CGCG 1 cut(s) 104
BshFI GGCC 1 cut(s) 450
BshNI GGYRCC 1 cut(s) 267
BsiSI CCGG 1 cut(s) 347
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 1 cut(s) 446
BsmAI GTCTC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 238
BsmI GAATGC 2 cut(s) 523, 709
BsnI GGCC 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 596
Bsp143I GATC 1 cut(s) 682
BspACI CCGC 2 cut(s) 104, 254
BspANI GGCC 1 cut(s) 450
BspFNI CGCG 1 cut(s) 104
BspLI GGNNCC 2 cut(s) 269, 593
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 1 cut(s) 677
BspT107I GGYRCC 1 cut(s) 267
BspTI CTTAAG 1 cut(s) 566
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 1 cut(s) 263
BssMI GATC 1 cut(s) 682
BssSI CACGAG 1 cut(s) 375
BssT1I CCWWGG 1 cut(s) 263
Bst2BI CACGAG 1 cut(s) 375
BstAFI CTTAAG 1 cut(s) 566
BstC8I GCNNGC 1 cut(s) 647
BstDEI CTNAG 2 cut(s) 587, 688
BstFNI CGCG 1 cut(s) 104
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 2 cut(s) 31, 217
BstKTI GATC 1 cut(s) 685
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 1 cut(s) 682
BstNSI RCATGY 2 cut(s) 245, 642
BstSCI CCNGG 1 cut(s) 346
BstSFI CTRYAG 1 cut(s) 366
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 1 cut(s) 352
BstV2I GAAGAC 1 cut(s) 620
BsuI GTATCC 2 cut(s) 423, 476
BsuRI GGCC 1 cut(s) 450
BtgZI GCGATG 1 cut(s) 589
BtsI GCAGTG 1 cut(s) 626
BtsIMutI CAGTG 2 cut(s) 615, 626
Cac8I GCNNGC 1 cut(s) 647
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 2 cut(s) 31, 217
CseI GACGC 1 cut(s) 625
Csp6I GTAC 3 cut(s) 239, 268, 380
CviAII CATG 3 cut(s) 35, 242, 639
CviJI RGCY 5 cut(s) 8, 262, 365, 450, 594
CviKI_1 RGCY 5 cut(s) 8, 262, 365, 450, 594
CviQI GTAC 3 cut(s) 239, 268, 380
DdeI CTNAG 2 cut(s) 587, 688
DpnI GATC 1 cut(s) 684
DpnII GATC 1 cut(s) 682
DraI TTTAAA 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 596
Eco32I GATATC 1 cut(s) 458
Eco47III AGCGCT 1 cut(s) 216
EcoRV GATATC 1 cut(s) 458
EcoT14I CCWWGG 1 cut(s) 263
EcoT38I GRGCYC 1 cut(s) 596
ErhI CCWWGG 1 cut(s) 263
FaeI CATG 3 cut(s) 38, 245, 642
FalI AAGNNNNNCTT 2 cut(s) 580, 612
FaqI GGGAC 1 cut(s) 238
FatI CATG 3 cut(s) 34, 241, 638
FblI GTMKAC 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 366
FriOI GRGCYC 1 cut(s) 596
Fsp4HI GCNGC 1 cut(s) 366
FspBI CTAG 3 cut(s) 218, 264, 534
FspI TGCGCA 1 cut(s) 30
GlaI GCGC 2 cut(s) 30, 216
GluI GCNGC 1 cut(s) 366
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 1 cut(s) 450
HapII CCGG 1 cut(s) 347
HgaI GACGC 1 cut(s) 625
HhaI GCGC 2 cut(s) 31, 217
Hin1II CATG 3 cut(s) 38, 245, 642
Hin6I GCGC 2 cut(s) 29, 215
HinP1I GCGC 2 cut(s) 29, 215
HinfI GANTC 3 cut(s) 48, 371, 758
HpaII CCGG 1 cut(s) 347
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 3 cut(s) 178, 212, 831
Hpy188III TCNNGA 2 cut(s) 164, 375
Hpy8I GTNNAC 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 473, 606, 660
HpyCH4V TGCA 3 cut(s) 19, 328, 368
HpyF3I CTNAG 2 cut(s) 587, 688
Hsp92II CATG 3 cut(s) 38, 245, 642
HspAI GCGC 2 cut(s) 29, 215
KpnI GGTACC 1 cut(s) 271
Kzo9I GATC 1 cut(s) 682
LmnI GCTCC 4 cut(s) 212, 320, 442, 591
LpnPI CCDG 7 cut(s) 165, 171, 177, 261, 360, 464, 621
Lsp1109I GCAGC 1 cut(s) 352
LweI GCATC 1 cut(s) 561
MaeI CTAG 3 cut(s) 218, 264, 534
MaeIII GTNAC 1 cut(s) 561
MalI GATC 1 cut(s) 684
MboI GATC 1 cut(s) 682
MboII GAAGA 3 cut(s) 292, 519, 625
MfeI CAATTG 1 cut(s) 137
MhlI GDGCHC 1 cut(s) 596
MlyI GAGTC 1 cut(s) 752
MnlI CCTC 4 cut(s) 349, 420, 440, 815
MroXI GAANNNNTTC 1 cut(s) 159
MseI TTAA 6 cut(s) 99, 110, 143, 464, 513, 567
MslI CAYNNNNRTG 1 cut(s) 465
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 1 cut(s) 348
MunI CAATTG 1 cut(s) 137
Mva1269I GAATGC 2 cut(s) 523, 709
MvnI CGCG 1 cut(s) 104
NciI CCSGG 1 cut(s) 348
NdeII GATC 1 cut(s) 682
NlaIII CATG 3 cut(s) 38, 245, 642
NlaIV GGNNCC 2 cut(s) 269, 593
NsbI TGCGCA 1 cut(s) 30
NspI RCATGY 2 cut(s) 245, 642
PciI ACATGT 1 cut(s) 241
PcsI WCGNNNNNNNCGW 1 cut(s) 382
PctI GAATGC 2 cut(s) 523, 709
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 48, 371
PkrI GCNGC 1 cut(s) 367
PleI GAGTC 1 cut(s) 752
PpsI GAGTC 1 cut(s) 752
PscI ACATGT 1 cut(s) 241
PsiI TTATAA 1 cut(s) 662
PspN4I GGNNCC 2 cut(s) 269, 593
PstI CTGCAG 1 cut(s) 370
PstNI CAGNNNCTG 1 cut(s) 158
RsaI GTAC 3 cut(s) 240, 269, 381
RsaNI GTAC 3 cut(s) 239, 268, 380
RseI CAYNNNNRTG 1 cut(s) 465
SaqAI TTAA 6 cut(s) 99, 110, 143, 464, 513, 567
SatI GCNGC 1 cut(s) 366
Sau3AI GATC 1 cut(s) 682
ScaI AGTACT 1 cut(s) 381
SchI GAGTC 1 cut(s) 752
ScrFI CCNGG 1 cut(s) 348
SduI GDGCHC 1 cut(s) 596
SetI ASST 6 cut(s) 160, 269, 399, 431, 493, 660
SfaNI GCATC 1 cut(s) 561
SfcI CTRYAG 1 cut(s) 366
SmiI ATTTAAAT 1 cut(s) 465
SmiMI CAYNNNNRTG 1 cut(s) 465
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
SsiI CCGC 2 cut(s) 104, 254
SspI AATATT 1 cut(s) 95
SspMI CTAG 3 cut(s) 218, 264, 534
StyD4I CCNGG 1 cut(s) 346
StyI CCWWGG 1 cut(s) 263
SwaI ATTTAAAT 1 cut(s) 465
TaqI TCGA 1 cut(s) 756
TatI WGTACW 2 cut(s) 238, 379
TfiI GAWTC 2 cut(s) 48, 371
Tru1I TTAA 6 cut(s) 99, 110, 143, 464, 513, 567
Tru9I TTAA 6 cut(s) 99, 110, 143, 464, 513, 567
TscAI CASTG 2 cut(s) 615, 626
TseI GCWGC 1 cut(s) 365
TspDTI ATGAA 6 cut(s) 51, 300, 322, 588, 711, 828
TspRI CASTG 2 cut(s) 615, 626
Vha464I CTTAAG 1 cut(s) 566
XceI RCATGY 2 cut(s) 245, 642
XmaJI CCTAGG 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 3 cut(s) 218, 264, 534
ZrmI AGTACT 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.