Rw1G026390
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000468
Physical Location & Seq
Reverse (-)
53537443 .. 53538956
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G026390.1

Sequence Viewer

Length: 1314 bp
ATGGATCGAAGGCAAATTATGTTGCTCTTATTGGTGGAGTTGTGGCACCTAGAGACAATGTGTGTGTGTACTATGCTTGTGCTACTGATGTTTAATTTGAGACGAAGACGTGCTGAAAGACATGCTCTAAATAATCGTTCATTCGTTAGACGTGAGACTCGTTTGAGTTATCTAGATAGTATTATAGGCAATAGTGACACCGAATGCGTACACGAATTAAGAATGGATAGAATGACTTTTGGCCTTTTGTGTGAACTGCTTCGCTCAGATGGAAATATAAAGAGTGATGGTTTGGTGTCTGTGGAAGAGCAAGTATGTATGTTTTTACATATACTTTCACATCATGTGAAGAATCGTACTATTAGAGGTAGATTTTTTCGATCCGGAGAGACTATTAGTAGGTATTTCCATTCTATACTACAAGGTATTTTACGATTGCAAGGTAGTTTGTTGAGGGTACCTGATCCGGTACTTGCTGACTGCACAGATCGTAGATGGAAGTGCTTTAAGAATTGCTTGGGGGCACTAGATGGAACTTACATTAGAGTGCGTGTACCTACAATTGACAAGCCAAGATACCGCACAAGAAAGGGTGAAGTCGCTACAAATGTGTTAGCTGCTTGTTCTCGTGATATGCAGTTCATATTTGTCTTACCGGGTTGGGAGGGATCTGCATCGGACTCTAGAGTTCTTCACGATGCAGTGACTAGGCCGAATGGGTTAAGAGTTCCCACTGGTTATTATTACCTTGTAGATGGTGGTTACACAAATGGTGAGGGATTTCTTGCACCATATAGAGGAACAAGGTATCATTTGTCGGAATGGGATGAAAGAAATATGCCTACTGATCATGTGGAATATTTTAACATGAAGCATTCCAAGGCAAGAAATGTGATAGAACGGTGTTTTGGAATGCTAAAAGGAAAGTGGGGAATACTAAGGAGTCCATCATTTTATCCAATAAAGACACAATGCCGCATAATTACGGTTTGTTGTCTTTTGCATAATTTTATTAGGAGAGAGATGTCAATTGATCCGATGGAGTATGCAGTATGTGAATTAGATGCACAAAATGGGGAAGAAGCTGATGTAGTTGGCACGGTTGAAGCATCTAATCAATGGACTACTTGGAGGAATGAATTAGCTTTACAAATGCATAATGAATGGACGGGAAGAAGGGCTGTAAGGGCTACCGGAGATACAGGGGTTTCAGGTGCTGCTGCAAGGGCTGCAAGGGTTGCAAGGGCTGCTAGAGCTGCAAGGAATGCAAGAGCTGCAGGGGATGGAAGAGCTGCTCCAGCTTCAAGAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

437

Amino Acids

49.98

Weight (kDa)

9.4

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 56 - 144 5.3e-24 Domain of unknown function (DUF8040)
DDE_Tnp_1 PF01609 172 - 334 2.2e-10 Transposase DDE domain
DDE_Tnp_4 PF13359 176 - 336 2.3e-28 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 457
AccB1I GGYRCC 2 cut(s) 45, 457
AccIII TCCGGA 1 cut(s) 383
AciI CCGC 2 cut(s) 580, 976
AclWI GGATC 5 cut(s) 12, 375, 458, 676, 1028
AfaI GTAC 6 cut(s) 70, 210, 358, 459, 471, 555
AfiI CCNNNNNNNGG 1 cut(s) 797
AgsI TTSAA 2 cut(s) 1106, 1305
AjiI CACGTC 2 cut(s) 110, 152
AjuI GAANNNNNNNTTGG 2 cut(s) 891, 923
AluBI AGCT 7 cut(s) 617, 1085, 1145, 1256, 1274, 1292, 1301
AluI AGCT 7 cut(s) 617, 1085, 1145, 1256, 1274, 1292, 1301
Alw26I GTCTC 4 cut(s) 47, 94, 149, 383
AlwI GGATC 5 cut(s) 12, 375, 458, 676, 1028
AlwNI CAGNNNCTG 1 cut(s) 1217
Aor13HI TCCGGA 1 cut(s) 383
AoxI GGCC 2 cut(s) 241, 710
ApeKI GCWGC 8 cut(s) 617, 1217, 1220, 1229, 1247, 1256, 1274, 1292
Asp700I GAANNNNTTC 1 cut(s) 258
Asp718I GGTACC 1 cut(s) 457
AsuC2I CCSGG 1 cut(s) 657
AsuHPI GGTGA 2 cut(s) 605, 785
BaeGI GKGCMC 1 cut(s) 526
BaeI ACNNNNGTAYC 2 cut(s) 1191, 1224
BanI GGYRCC 2 cut(s) 45, 457
BauI CACGAG 1 cut(s) 627
BbsI GAAGAC 1 cut(s) 112
BbvI GCAGC 8 cut(s) 604, 1204, 1207, 1216, 1234, 1243, 1261, 1279
BccI CCATC 8 cut(s) 263, 281, 489, 524, 749, 955, 1033, 1277
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 1 cut(s) 657
BcoDI GTCTC 4 cut(s) 47, 94, 149, 383
BfaI CTAG 6 cut(s) 50, 173, 527, 684, 708, 1251
BfmI CTRYAG 1 cut(s) 1275
BisI GCNGC 9 cut(s) 618, 976, 1218, 1221, 1230, 1248, 1257, 1275, 1293
BlsI GCNGC 9 cut(s) 619, 977, 1219, 1222, 1231, 1249, 1258, 1276, 1294
Bme1390I CCNGG 1 cut(s) 657
BmgBI CACGTC 2 cut(s) 110, 152
BmiI GGNNCC 2 cut(s) 47, 459
BmrFI CCNGG 1 cut(s) 657
BmsI GCATC 4 cut(s) 683, 688, 1054, 1118
BpiI GAAGAC 1 cut(s) 112
BpmI CTGGAG 1 cut(s) 1281
BpuMI CCSGG 1 cut(s) 657
BsaBI GATNNNNATC 1 cut(s) 673
BsaJI CCNNGG 1 cut(s) 879
BsaWI WCCGGW 3 cut(s) 383, 466, 1193
Bsc4I CCNNNNNNNGG 1 cut(s) 797
Bse1I ACTGG 1 cut(s) 739
Bse8I GATNNNNATC 1 cut(s) 673
BseAI TCCGGA 1 cut(s) 383
BseDI CCNNGG 1 cut(s) 879
BseGI GGATG 2 cut(s) 832, 1288
BseJI GATNNNNATC 1 cut(s) 673
BseLI CCNNNNNNNGG 1 cut(s) 797
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 739
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 8 cut(s) 604, 1204, 1207, 1216, 1234, 1243, 1261, 1279
BsgI GTGCAG 1 cut(s) 466
BshFI GGCC 2 cut(s) 243, 712
BshNI GGYRCC 2 cut(s) 45, 457
BsiSI CCGG 4 cut(s) 384, 467, 656, 1194
BslI CCNNNNNNNGG 1 cut(s) 797
BsmAI GTCTC 4 cut(s) 47, 94, 149, 383
BsmBI CGTCTC 1 cut(s) 94
BsmI GAATGC 4 cut(s) 209, 874, 918, 1270
BsnI GGCC 2 cut(s) 243, 712
Bsp1286I GDGCHC 1 cut(s) 526
Bsp13I TCCGGA 1 cut(s) 383
Bsp143I GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
BspACI CCGC 2 cut(s) 580, 976
BspANI GGCC 2 cut(s) 243, 712
BspCNI CTCAG 1 cut(s) 278
BspEI TCCGGA 1 cut(s) 383
BspLI GGNNCC 2 cut(s) 47, 459
BspMAI CTGCAG 1 cut(s) 1279
BspPI GGATC 5 cut(s) 12, 375, 458, 676, 1028
BspQI GCTCTTC 2 cut(s) 300, 1282
BspT107I GGYRCC 2 cut(s) 45, 457
BsrI ACTGG 1 cut(s) 739
BssECI CCNNGG 1 cut(s) 879
BssMI GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
BssSI CACGAG 1 cut(s) 627
BssT1I CCWWGG 1 cut(s) 879
Bst2BI CACGAG 1 cut(s) 627
Bst4CI ACNGT 3 cut(s) 903, 988, 1102
Bst6I CTCTTC 2 cut(s) 300, 1282
BstAPI GCANNNNNTGC 5 cut(s) 1229, 1238, 1247, 1265, 1274
BstDEI CTNAG 2 cut(s) 265, 938
BstF5I GGATG 2 cut(s) 832, 1288
BstKTI GATC 7 cut(s) 7, 383, 466, 490, 671, 850, 1036
BstMAI GTCTC 4 cut(s) 47, 94, 149, 383
BstMBI GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 655
BstSFI CTRYAG 1 cut(s) 1275
BstSLI GKGCMC 1 cut(s) 526
BstV1I GCAGC 8 cut(s) 604, 1204, 1207, 1216, 1234, 1243, 1261, 1279
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
BsuRI GGCC 2 cut(s) 243, 712
BtrI CACGTC 2 cut(s) 110, 152
BtsCI GGATG 2 cut(s) 832, 1288
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 2 cut(s) 708, 732
CaiI CAGNNNCTG 1 cut(s) 1217
Csp6I GTAC 6 cut(s) 69, 209, 357, 458, 470, 554
CviAII CATG 4 cut(s) 122, 344, 851, 868
CviQI GTAC 6 cut(s) 69, 209, 357, 458, 470, 554
DdeI CTNAG 2 cut(s) 265, 938
DpnI GATC 7 cut(s) 6, 382, 465, 489, 670, 849, 1035
DpnII GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
Eam1104I CTCTTC 2 cut(s) 300, 1282
EarI CTCTTC 2 cut(s) 300, 1282
Eco130I CCWWGG 1 cut(s) 879
EcoT14I CCWWGG 1 cut(s) 879
EcoT22I ATGCAT 1 cut(s) 1158
ErhI CCWWGG 1 cut(s) 879
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 4 cut(s) 125, 347, 854, 871
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FatI CATG 4 cut(s) 121, 343, 850, 867
FbaI TGATCA 1 cut(s) 847
Fnu4HI GCNGC 9 cut(s) 618, 976, 1218, 1221, 1230, 1248, 1257, 1275, 1293
FokI GGATG 2 cut(s) 839, 1295
Fsp4HI GCNGC 9 cut(s) 618, 976, 1218, 1221, 1230, 1248, 1257, 1275, 1293
FspBI CTAG 6 cut(s) 50, 173, 527, 684, 708, 1251
GluI GCNGC 9 cut(s) 618, 976, 1218, 1221, 1230, 1248, 1257, 1275, 1293
GsuI CTGGAG 1 cut(s) 1281
HaeIII GGCC 2 cut(s) 243, 712
HapII CCGG 4 cut(s) 384, 467, 656, 1194
Hin1II CATG 4 cut(s) 125, 347, 854, 871
HinfI GANTC 4 cut(s) 157, 352, 680, 943
HpaII CCGG 4 cut(s) 384, 467, 656, 1194
HphI GGTGA 2 cut(s) 605, 785
Hpy166II GTNNAC 4 cut(s) 69, 211, 254, 554
Hpy188I TCNGA 4 cut(s) 268, 679, 820, 1038
Hpy188III TCNNGA 6 cut(s) 173, 384, 629, 684, 695, 1305
Hpy8I GTNNAC 4 cut(s) 69, 211, 254, 554
HpyAV CCTTC 2 cut(s) 3, 1170
HpyCH4III ACNGT 3 cut(s) 903, 988, 1102
HpyCH4IV ACGT 2 cut(s) 109, 151
HpyF3I CTNAG 2 cut(s) 265, 938
HpySE526I ACGT 2 cut(s) 109, 151
Hsp92II CATG 4 cut(s) 125, 347, 854, 871
Kpn2I TCCGGA 1 cut(s) 383
KpnI GGTACC 1 cut(s) 461
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
LguI GCTCTTC 2 cut(s) 300, 1282
LmnI GCTCC 1 cut(s) 1300
LpnPI CCDG 9 cut(s) 397, 474, 480, 669, 720, 1188, 1197, 1207, 1263
Lsp1109I GCAGC 8 cut(s) 604, 1204, 1207, 1216, 1234, 1243, 1261, 1279
LweI GCATC 4 cut(s) 683, 688, 1054, 1118
MaeI CTAG 6 cut(s) 50, 173, 527, 684, 708, 1251
MaeII ACGT 2 cut(s) 109, 151
MaeIII GTNAC 3 cut(s) 194, 703, 761
MalI GATC 7 cut(s) 6, 382, 465, 489, 670, 849, 1035
MboI GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
MboII GAAGA 7 cut(s) 117, 317, 361, 683, 1091, 1185, 1299
MfeI CAATTG 2 cut(s) 561, 1029
MflI RGATCY 1 cut(s) 668
MhlI GDGCHC 1 cut(s) 526
MlyI GAGTC 3 cut(s) 151, 674, 952
MmeI TCCRAC 1 cut(s) 798
MnlI CCTC 6 cut(s) 359, 447, 658, 769, 791, 1125
Mph1103I ATGCAT 1 cut(s) 1158
MroI TCCGGA 1 cut(s) 383
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 5 cut(s) 93, 218, 507, 722, 864
MslI CAYNNNNRTG 1 cut(s) 545
MspI CCGG 4 cut(s) 384, 467, 656, 1194
MspR9I CCNGG 1 cut(s) 657
MunI CAATTG 2 cut(s) 561, 1029
Mva1269I GAATGC 4 cut(s) 209, 874, 918, 1270
NciI CCSGG 1 cut(s) 657
NdeII GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
NlaIII CATG 4 cut(s) 125, 347, 854, 871
NlaIV GGNNCC 2 cut(s) 47, 459
NmuCI GTSAC 2 cut(s) 194, 703
NsiI ATGCAT 1 cut(s) 1158
NspI RCATGY 1 cut(s) 125
PciSI GCTCTTC 2 cut(s) 300, 1282
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 4 cut(s) 209, 874, 918, 1270
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 1 cut(s) 352
PkrI GCNGC 9 cut(s) 619, 977, 1219, 1222, 1231, 1249, 1258, 1276, 1294
PleI GAGTC 3 cut(s) 151, 674, 951
PpsI GAGTC 3 cut(s) 151, 674, 951
PspN4I GGNNCC 2 cut(s) 47, 459
PstI CTGCAG 1 cut(s) 1279
PstNI CAGNNNCTG 1 cut(s) 1217
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 6 cut(s) 70, 210, 358, 459, 471, 555
RsaNI GTAC 6 cut(s) 69, 209, 357, 458, 470, 554
RseI CAYNNNNRTG 1 cut(s) 545
SapI GCTCTTC 2 cut(s) 300, 1282
SaqAI TTAA 5 cut(s) 93, 218, 507, 722, 864
SatI GCNGC 9 cut(s) 618, 976, 1218, 1221, 1230, 1248, 1257, 1275, 1293
Sau3AI GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
SchI GAGTC 3 cut(s) 151, 674, 952
ScrFI CCNGG 1 cut(s) 657
SduI GDGCHC 1 cut(s) 526
SfaNI GCATC 4 cut(s) 683, 688, 1054, 1118
SfcI CTRYAG 1 cut(s) 1275
SmiMI CAYNNNNRTG 1 cut(s) 545
SsiI CCGC 2 cut(s) 580, 976
SspI AATATT 1 cut(s) 860
SspMI CTAG 6 cut(s) 50, 173, 527, 684, 708, 1251
StyD4I CCNGG 1 cut(s) 655
StyI CCWWGG 1 cut(s) 879
TaaI ACNGT 3 cut(s) 903, 988, 1102
TaiI ACGT 2 cut(s) 112, 154
TaqI TCGA 2 cut(s) 7, 379
TatI WGTACW 1 cut(s) 68
TauI GCSGC 1 cut(s) 978
TfiI GAWTC 1 cut(s) 352
Tru1I TTAA 5 cut(s) 93, 218, 507, 722, 864
Tru9I TTAA 5 cut(s) 93, 218, 507, 722, 864
TscAI CASTG 2 cut(s) 708, 739
TseFI GTSAC 2 cut(s) 194, 703
TseI GCWGC 8 cut(s) 617, 1217, 1220, 1229, 1247, 1256, 1274, 1292
Tsp45I GTSAC 2 cut(s) 194, 703
TspDTI ATGAA 6 cut(s) 129, 631, 843, 884, 1152, 1176
TspRI CASTG 2 cut(s) 708, 739
XbaI TCTAGA 2 cut(s) 172, 683
XceI RCATGY 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 6 cut(s) 50, 173, 527, 684, 708, 1251
Zsp2I ATGCAT 1 cut(s) 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.