Rorug01G0237500
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000468
Physical Location & Seq
Forward (+)
34221227 .. 34226142
4916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0237500.1

Sequence Viewer

Length: 1338 bp
ATGGTTACTGTCAAAGATGCTGTGGGGTCTTTTGTTGCTTGGCCGAGGGATCTTATAGTTCAATCCCCTCCCGATGTTAAGAAGAAACAACCTGTGAAGGCTGGTAAGGGTTTGAAGAAGAGGAAAAGGGTAGTAGCCGTTGATGAAGAGGAGTTATTTATCGACTTCAAGAAGCTGCCGAAAGATTACCCTGCAGCGTGCAAGTGCTTGTGGTTGTGGGCAAAAGACAATTTGGCAAATGGGAAGACAATCTCCTTTCAGATGGAAGACAAAGTTTTTGGCATCAACAAAAAGCAATATGTGTTTAAGGCAGATATTCATGCCTTGTGTACCATGGGTGAATTGTCGGGTGGAGTGATCTGCATGTACATGCTCTACTTGCATGAAGTCTTGAAGAGAGCAAAGATGTCAGAGATGGTTGGCTTTGTGGACCCTTCACAGCTTGGCGCACTTGGATGTGGAAATCCAACAGAAAGATCACGCCATCTAGCAAATAGGTTCCAAAATGCAAAGAAAGGGCAGATTTTTATGTTGCCATATAATGCAGGCAAGCACTGGATGTTGACTGTTGTCAATCCGGATGAAGAAACAATCTACTTCATGGATCCTTTGAGGCGTCGACTAATAGGTGACGAATGGGAGACGGTTGTGGAGAATGGTATCAAAATTTACAATGCTCACAGGAATAGATCTAGCCGCAAATCAATATTGTGGCAAAACATGGCGGGTATTCCGGTGCAGCAAACTGACAAGGATTGTGCCATATTTATCATGCGGTTCATGAAAGAAATAGTTGAGGATAGGAATCTGGAATTTGCATCAAAATGGCAGCGGCGATCATCCAAGGAACTGGTTTACACCCAAAAGGATCTTGATGCTGTGAGGACAGAGTGGGCTAATGCCCGGCGTCTTGCTGGGGTTGATGATCCTAACAAGTCTTCGGAAAAGCTTCACTTCACCAACAAGGGTACTGATGTTAACATTACAGTTGTGCCGAAAGTGAAATCTTTCTCGTCATCAAAGCCGGTTCTTGAAGTTACAGAAGGATCAGAAATCACGGTGGAGACGGAACCCGATAGCGATGATAGTACTAGAGAAGAAAGTCCTAAACGGTACCACACAAAAGCTACCCCCAATGTGAAGAATGTCTCATCGAGAAAAAGGAAAGGATCAACCTCATCATCCTCAAGTGCTGCAGATCAGAAGTCGAAGACAAGGTCTGATCAGCATGTTTCAGTTTCATGGCGAGTGCCGCGAAACAAACGAGGGATTCAGAAGCAACCAGGGTTTAATCTGGACTATTCACCCCCGAAGACACACCCTCCTTCTCATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

445

Amino Acids

50.61

Weight (kDa)

9.6

Isoelectric Point (pI)

52.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 169 - 300 2.6e-11 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1113
AccB1I GGYRCC 1 cut(s) 1113
AccI GTMKAC 1 cut(s) 619
AccII CGCG 1 cut(s) 1255
AccIII TCCGGA 1 cut(s) 577
AciI CCGC 5 cut(s) 697, 725, 775, 832, 1253
AclWI GGATC 7 cut(s) 57, 599, 612, 876, 920, 1054, 1177
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 2 cut(s) 666, 812
AcyI GRCGYC 2 cut(s) 616, 907
AfaI GTAC 5 cut(s) 331, 368, 970, 1090, 1115
AgsI TTSAA 5 cut(s) 62, 115, 169, 394, 1034
AjnI CCWGG 1 cut(s) 1282
AluBI AGCT 4 cut(s) 175, 442, 949, 1127
AluI AGCT 4 cut(s) 175, 442, 949, 1127
Alw26I GTCTC 3 cut(s) 635, 1058, 1153
AlwI GGATC 7 cut(s) 57, 599, 612, 876, 920, 1054, 1177
Aor13HI TCCGGA 1 cut(s) 577
AoxI GGCC 1 cut(s) 41
ApeKI GCWGC 5 cut(s) 175, 194, 739, 829, 1193
ApoI RAATTY 2 cut(s) 666, 812
ArsI GACNNNNNNTTYG 4 cut(s) 260, 292, 1202, 1234
Asp700I GAANNNNTTC 2 cut(s) 948, 1007
Asp718I GGTACC 1 cut(s) 1113
AspLEI GCGC 1 cut(s) 449
AspS9I GGNCC 1 cut(s) 430
AsuC2I CCSGG 1 cut(s) 904
AsuHPI GGTGA 4 cut(s) 350, 641, 949, 1296
AvaII GGWCC 1 cut(s) 430
BaeI ACNNNNGTAYC 2 cut(s) 960, 993
BamHI GGATCC 1 cut(s) 604
BanI GGYRCC 1 cut(s) 1113
BbsI GAAGAC 5 cut(s) 251, 273, 930, 1217, 1319
BbvI GCAGC 5 cut(s) 162, 206, 751, 841, 1180
BccI CCATC 3 cut(s) 256, 409, 492
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 1284
BclI TGATCA 1 cut(s) 1222
BcnI CCSGG 1 cut(s) 904
BcoDI GTCTC 3 cut(s) 635, 1058, 1153
BfaI CTAG 3 cut(s) 488, 693, 1092
BfmI CTRYAG 2 cut(s) 192, 1194
BglII AGATCT 1 cut(s) 689
BisI GCNGC 8 cut(s) 176, 195, 697, 740, 830, 833, 1194, 1253
BlsI GCNGC 8 cut(s) 177, 196, 698, 741, 831, 834, 1195, 1254
BmcAI AGTACT 1 cut(s) 1090
Bme1390I CCNGG 2 cut(s) 904, 1284
Bme18I GGWCC 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 430
BmiI GGNNCC 5 cut(s) 432, 500, 606, 1071, 1115
BmrFI CCNGG 2 cut(s) 904, 1284
BmsI GCATC 4 cut(s) 7, 291, 827, 865
BoxI GACNNNNGTC 1 cut(s) 569
BpiI GAAGAC 5 cut(s) 251, 273, 930, 1217, 1319
BpuEI CTTGAG 1 cut(s) 1171
BpuMI CCSGG 1 cut(s) 904
BsaBI GATNNNNATC 1 cut(s) 804
BsaHI GRCGYC 2 cut(s) 616, 907
BsaJI CCNNGG 4 cut(s) 44, 333, 843, 1283
BsaWI WCCGGW 2 cut(s) 577, 733
BsaXI ACNNNNNCTCC 2 cut(s) 1306, 1336
Bse118I RCCGGY 1 cut(s) 1024
Bse1I ACTGG 2 cut(s) 560, 855
Bse8I GATNNNNATC 1 cut(s) 804
BseAI TCCGGA 1 cut(s) 577
BseBI CCWGG 1 cut(s) 1284
BseDI CCNNGG 4 cut(s) 44, 333, 843, 1283
BseGI GGATG 5 cut(s) 461, 564, 586, 839, 1181
BseJI GATNNNNATC 1 cut(s) 804
BseNI ACTGG 2 cut(s) 560, 855
BseRI GAGGAG 1 cut(s) 164
BseXI GCAGC 5 cut(s) 162, 206, 751, 841, 1180
BseYI CCCAGC 1 cut(s) 914
BsgI GTGCAG 1 cut(s) 758
Bsh1236I CGCG 1 cut(s) 1255
BshFI GGCC 1 cut(s) 43
BshNI GGYRCC 1 cut(s) 1113
BsiSI CCGG 4 cut(s) 578, 734, 904, 1025
BsmAI GTCTC 3 cut(s) 635, 1058, 1153
BsmBI CGTCTC 2 cut(s) 635, 1058
BsnI GGCC 1 cut(s) 43
Bsp13I TCCGGA 1 cut(s) 577
Bsp1407I TGTACA 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 5 cut(s) 697, 725, 775, 832, 1253
BspANI GGCC 1 cut(s) 43
BspEI TCCGGA 1 cut(s) 577
BspFNI CGCG 1 cut(s) 1255
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 5 cut(s) 432, 500, 606, 1071, 1115
BspMAI CTGCAG 2 cut(s) 196, 1198
BspPI GGATC 7 cut(s) 57, 599, 612, 876, 920, 1054, 1177
BspT107I GGYRCC 1 cut(s) 1113
BsrFI RCCGGY 1 cut(s) 1024
BsrGI TGTACA 1 cut(s) 366
BsrI ACTGG 2 cut(s) 560, 855
BssAI RCCGGY 1 cut(s) 1024
BssECI CCNNGG 4 cut(s) 44, 333, 843, 1283
BssNI GRCGYC 2 cut(s) 616, 907
BssT1I CCWWGG 2 cut(s) 333, 843
Bst2UI CCWGG 1 cut(s) 1284
Bst4CI ACNGT 6 cut(s) 10, 568, 646, 988, 1060, 1113
Bst6I CTCTTC 3 cut(s) 113, 141, 389
BstACI GRCGYC 2 cut(s) 616, 907
BstAUI TGTACA 1 cut(s) 366
BstC8I GCNNGC 3 cut(s) 199, 547, 551
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 5 cut(s) 461, 564, 586, 839, 1181
BstFNI CGCG 1 cut(s) 1255
BstHHI GCGC 1 cut(s) 449
BstMAI GTCTC 3 cut(s) 635, 1058, 1153
BstMWI GCNNNNNNNGC 2 cut(s) 379, 1252
BstNI CCWGG 1 cut(s) 1284
BstNSI RCATGY 3 cut(s) 367, 373, 1232
BstPAI GACNNNNGTC 1 cut(s) 569
BstSCI CCNGG 2 cut(s) 902, 1282
BstSFI CTRYAG 2 cut(s) 192, 1194
BstUI CGCG 1 cut(s) 1255
BstV1I GCAGC 5 cut(s) 162, 206, 751, 841, 1180
BstV2I GAAGAC 5 cut(s) 251, 273, 930, 1217, 1319
BstX2I RGATCY 4 cut(s) 49, 604, 689, 868
BstXI CCANNNNNNTGG 1 cut(s) 850
BstYI RGATCY 4 cut(s) 49, 604, 689, 868
BsuRI GGCC 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 333
BtgZI GCGATG 1 cut(s) 1095
BtsCI GGATG 5 cut(s) 461, 564, 586, 839, 1181
BtsIMutI CAGTG 1 cut(s) 553
Cac8I GCNNGC 3 cut(s) 199, 547, 551
CciI TCATGA 1 cut(s) 780
CfoI GCGC 1 cut(s) 449
Cfr10I RCCGGY 1 cut(s) 1024
Cfr13I GGNCC 1 cut(s) 430
CseI GACGC 2 cut(s) 605, 896
Csp6I GTAC 5 cut(s) 330, 367, 969, 1089, 1114
CviQI GTAC 5 cut(s) 330, 367, 969, 1089, 1114
EaeI YGGCCR 1 cut(s) 41
Eam1104I CTCTTC 3 cut(s) 113, 141, 389
EarI CTCTTC 3 cut(s) 113, 141, 389
Eco130I CCWWGG 2 cut(s) 333, 843
Eco47I GGWCC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 1282
EcoT14I CCWWGG 2 cut(s) 333, 843
ErhI CCWWGG 2 cut(s) 333, 843
Esp3I CGTCTC 2 cut(s) 635, 1058
FalI AAGNNNNNCTT 2 cut(s) 938, 970
FauI CCCGC 1 cut(s) 718
FbaI TGATCA 1 cut(s) 1222
FblI GTMKAC 1 cut(s) 619
Fnu4HI GCNGC 8 cut(s) 176, 195, 697, 740, 830, 833, 1194, 1253
FokI GGATG 5 cut(s) 468, 571, 593, 826, 1168
Fsp4HI GCNGC 8 cut(s) 176, 195, 697, 740, 830, 833, 1194, 1253
FspBI CTAG 3 cut(s) 488, 693, 1092
GlaI GCGC 1 cut(s) 448
GluI GCNGC 8 cut(s) 176, 195, 697, 740, 830, 833, 1194, 1253
GsaI CCCAGC 1 cut(s) 918
HaeIII GGCC 1 cut(s) 43
HapII CCGG 4 cut(s) 578, 734, 904, 1025
HgaI GACGC 2 cut(s) 605, 896
HhaI GCGC 1 cut(s) 449
Hin1I GRCGYC 2 cut(s) 616, 907
Hin6I GCGC 1 cut(s) 447
HinP1I GCGC 1 cut(s) 447
HincII GTYRAC 3 cut(s) 564, 620, 979
HindII GTYRAC 3 cut(s) 564, 620, 979
HindIII AAGCTT 1 cut(s) 947
HinfI GANTC 2 cut(s) 805, 1270
HpaI GTTAAC 1 cut(s) 979
HpaII CCGG 4 cut(s) 578, 734, 904, 1025
HphI GGTGA 4 cut(s) 350, 641, 949, 1296
Hpy166II GTNNAC 6 cut(s) 330, 430, 564, 620, 856, 979
Hpy188I TCNGA 7 cut(s) 261, 412, 943, 1051, 1203, 1222, 1275
Hpy8I GTNNAC 6 cut(s) 330, 430, 564, 620, 856, 979
Hpy99I CGWCG 1 cut(s) 621
HpyAV CCTTC 4 cut(s) 91, 444, 1037, 1335
HpyCH4III ACNGT 6 cut(s) 10, 568, 646, 988, 1060, 1113
HpyCH4V TGCA 9 cut(s) 194, 201, 363, 382, 509, 545, 739, 818, 1196
HpyF10VI GCNNNNNNNGC 2 cut(s) 379, 1252
Hsp92I GRCGYC 2 cut(s) 616, 907
HspAI GCGC 1 cut(s) 447
Kpn2I TCCGGA 1 cut(s) 577
KpnI GGTACC 1 cut(s) 1117
Ksp22I TGATCA 1 cut(s) 1222
KspAI GTTAAC 1 cut(s) 979
Lsp1109I GCAGC 5 cut(s) 162, 206, 751, 841, 1180
LweI GCATC 4 cut(s) 7, 291, 827, 865
MaeI CTAG 3 cut(s) 488, 693, 1092
MaeIII GTNAC 3 cut(s) 4, 629, 1036
MflI RGATCY 4 cut(s) 49, 604, 689, 868
MluCI AATT 4 cut(s) 229, 341, 666, 812
MmeI TCCRAC 1 cut(s) 491
MroI TCCGGA 1 cut(s) 577
MroXI GAANNNNTTC 2 cut(s) 948, 1007
MseI TTAA 4 cut(s) 78, 306, 978, 1290
MslI CAYNNNNRTG 3 cut(s) 368, 454, 823
MspA1I CMGCKG 1 cut(s) 832
MspI CCGG 4 cut(s) 578, 734, 904, 1025
MspR9I CCNGG 2 cut(s) 904, 1284
MvaI CCWGG 1 cut(s) 1284
MvnI CGCG 1 cut(s) 1255
MwoI GCNNNNNNNGC 2 cut(s) 379, 1252
NciI CCSGG 1 cut(s) 904
NcoI CCATGG 1 cut(s) 333
NlaIV GGNNCC 5 cut(s) 432, 500, 606, 1071, 1115
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 629
NspI RCATGY 3 cut(s) 367, 373, 1232
PagI TCATGA 1 cut(s) 780
PdmI GAANNNNTTC 2 cut(s) 948, 1007
PfeI GAWTC 2 cut(s) 805, 1270
PflFI GACNNNGTC 1 cut(s) 1216
PkrI GCNGC 8 cut(s) 177, 196, 698, 741, 831, 834, 1195, 1254
PshAI GACNNNNGTC 1 cut(s) 569
Psp6I CCWGG 1 cut(s) 1282
PspFI CCCAGC 1 cut(s) 914
PspGI CCWGG 1 cut(s) 1282
PspN4I GGNNCC 5 cut(s) 432, 500, 606, 1071, 1115
PspPI GGNCC 1 cut(s) 430
PstI CTGCAG 2 cut(s) 196, 1198
PsuI RGATCY 4 cut(s) 49, 604, 689, 868
PsyI GACNNNGTC 1 cut(s) 1216
RsaI GTAC 5 cut(s) 331, 368, 970, 1090, 1115
RsaNI GTAC 5 cut(s) 330, 367, 969, 1089, 1114
RseI CAYNNNNRTG 3 cut(s) 368, 454, 823
SalI GTCGAC 1 cut(s) 618
SaqAI TTAA 4 cut(s) 78, 306, 978, 1290
SatI GCNGC 8 cut(s) 176, 195, 697, 740, 830, 833, 1194, 1253
Sau96I GGNCC 1 cut(s) 430
ScaI AGTACT 1 cut(s) 1090
ScrFI CCNGG 2 cut(s) 904, 1284
SetI ASST 9 cut(s) 94, 177, 444, 500, 631, 951, 1129, 1178, 1220
SfaNI GCATC 4 cut(s) 7, 291, 827, 865
SfcI CTRYAG 2 cut(s) 192, 1194
SinI GGWCC 1 cut(s) 430
SmiMI CAYNNNNRTG 3 cut(s) 368, 454, 823
SmlI CTYRAG 1 cut(s) 1186
SmoI CTYRAG 1 cut(s) 1186
Sse9I AATT 4 cut(s) 229, 341, 666, 812
SsiI CCGC 5 cut(s) 697, 725, 775, 832, 1253
SspI AATATT 1 cut(s) 708
SspMI CTAG 3 cut(s) 488, 693, 1092
StyD4I CCNGG 2 cut(s) 902, 1282
StyI CCWWGG 2 cut(s) 333, 843
TaaI ACNGT 6 cut(s) 10, 568, 646, 988, 1060, 1113
TaqI TCGA 4 cut(s) 162, 619, 1154, 1208
TasI AATT 4 cut(s) 229, 341, 666, 812
TatI WGTACW 2 cut(s) 366, 1088
TauI GCSGC 3 cut(s) 699, 835, 1255
TfiI GAWTC 2 cut(s) 805, 1270
Tru1I TTAA 4 cut(s) 78, 306, 978, 1290
Tru9I TTAA 4 cut(s) 78, 306, 978, 1290
TscAI CASTG 1 cut(s) 560
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 5 cut(s) 175, 194, 739, 829, 1193
Tsp45I GTSAC 1 cut(s) 629
TspDTI ATGAA 8 cut(s) 159, 308, 399, 589, 597, 769, 797, 1230
TspGWI ACGGA 1 cut(s) 1082
TspRI CASTG 1 cut(s) 560
Tth111I GACNNNGTC 1 cut(s) 1216
VpaK11BI GGWCC 1 cut(s) 430
XapI RAATTY 2 cut(s) 666, 812
XceI RCATGY 3 cut(s) 367, 373, 1232
XmiI GTMKAC 1 cut(s) 619
XmnI GAANNNNTTC 2 cut(s) 948, 1007
XspI CTAG 3 cut(s) 488, 693, 1092
ZrmI AGTACT 1 cut(s) 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.