MD05G1202800.v1.1
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000468
Physical Location & Seq
Reverse (-)
33205008 .. 33207263
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1202800.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGATGAAAGAAAGATTGTGGCATGCTTCATTGTTAAGTTGTGGTTTTGTCGATCGGTGGAGATCATAAGTAGGTATTTCAATTCCATTCTACATGGAGTGTTAAGGCTACAAGGTAGACTTTTGAAAGTGCCAGAACCCATTCCTAATGCCTACCCAGATCCTAAATGGAGTTGGTTTGAGGGTGTTACTACCTTGTGGATGGTGGGTACACAAATGGTCCAGGATACCTTGCACCGTATAGGGGAGTGCGTTACCATCTCTCAGTGGAGAAATAGAAGACTCCCAATTAATCATGAGGAGTATTTCAATATGAAACATGCTCAAGCTAGAAATGTCATTGAGAGATGTTTTGGACTACTTAAGATGCGTTTGAGCATCTTAAGAGGTCCATCATTTTTCCCAATTAAAACACAACTTAGAATTATGACAACCTGCTGCATATTACATAATCTTATTAGAAGGCATATGTCAGCTGATCCAATAAAAAATGAAATCCTAAACTTGGACGAATCTGAAAGTAGTGATGATGATGAAGATATGATTGAGATTGTTCAACTTACACAAGAATGGACTGCATGGAGAAATACTTTGGCCATGAATATGTACAATGAGTGGAATGCACAAGTTAACAAAGAAGCAGAGGGCTGGCTAAGAAAATTGTTTCCATTGTATGACAGATTGAATGTTATTTTTGGGAAAGACCGAGCAACTGGAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

241

Amino Acids

28.38

Weight (kDa)

8.53

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 97 - 151 4e-09 DDE superfamily endonuclease
Plant_tran PF04827 98 - 153 1.4e-07 Plant transposon protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 443
AccI GTMKAC 1 cut(s) 118
AclWI GGATC 2 cut(s) 155, 473
AcoI YGGCCR 1 cut(s) 594
AfaI GTAC 2 cut(s) 211, 608
AfiI CCNNNNNNNGG 2 cut(s) 244, 505
AflII CTTAAG 2 cut(s) 362, 382
AgsI TTSAA 5 cut(s) 82, 127, 310, 557, 685
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 2 cut(s) 329, 476
AluI AGCT 2 cut(s) 329, 476
AlwI GGATC 2 cut(s) 155, 473
AoxI GGCC 1 cut(s) 594
ApeKI GCWGC 1 cut(s) 438
AseI ATTAAT 1 cut(s) 291
Asp700I GAANNNNTTC 1 cut(s) 141
AspS9I GGNCC 2 cut(s) 220, 389
AvaII GGWCC 2 cut(s) 220, 389
BalI TGGCCA 1 cut(s) 596
BbsI GAAGAC 1 cut(s) 286
BbvI GCAGC 1 cut(s) 425
BccI CCATC 3 cut(s) 196, 266, 400
BciT130I CCWGG 1 cut(s) 224
BciVI GTATCC 1 cut(s) 220
BfaI CTAG 1 cut(s) 330
BfrI CTTAAG 2 cut(s) 362, 382
BfuAI ACCTGC 1 cut(s) 443
BfuI GTATCC 1 cut(s) 220
BisI GCNGC 1 cut(s) 439
BlsI GCNGC 1 cut(s) 440
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 2 cut(s) 220, 389
BmgT120I GGNCC 2 cut(s) 220, 389
BmrFI CCNGG 1 cut(s) 224
BmsI GCATC 2 cut(s) 357, 387
BpiI GAAGAC 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 309
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 505
Bse1I ACTGG 1 cut(s) 718
BseBI CCWGG 1 cut(s) 224
BseGI GGATG 2 cut(s) 10, 207
BseLI CCNNNNNNNGG 2 cut(s) 244, 505
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 1 cut(s) 718
BseRI GAGGAG 1 cut(s) 314
BseXI GCAGC 1 cut(s) 425
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 1 cut(s) 596
BsiEI CGRYCG 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 244, 505
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 1 cut(s) 596
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 4 cut(s) 53, 63, 160, 478
BspANI GGCC 1 cut(s) 596
BspCNI CTCAG 1 cut(s) 277
BspHI TCATGA 1 cut(s) 295
BspMI ACCTGC 1 cut(s) 443
BspPI GGATC 2 cut(s) 155, 473
BspTI CTTAAG 2 cut(s) 362, 382
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 1 cut(s) 718
BssMI GATC 4 cut(s) 53, 63, 160, 478
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 1 cut(s) 239
BstAFI CTTAAG 2 cut(s) 362, 382
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 2 cut(s) 25, 650
BstDEI CTNAG 3 cut(s) 264, 419, 653
BstF5I GGATG 2 cut(s) 10, 207
BstKTI GATC 4 cut(s) 56, 66, 163, 481
BstMBI GATC 4 cut(s) 53, 63, 160, 478
BstMCI CGRYCG 1 cut(s) 56
BstNI CCWGG 1 cut(s) 224
BstNSI RCATGY 2 cut(s) 27, 323
BstSCI CCNGG 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 425
BstV2I GAAGAC 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuI GTATCC 1 cut(s) 220
BsuRI GGCC 1 cut(s) 596
BtsCI GGATG 2 cut(s) 10, 207
BtsIMutI CAGTG 1 cut(s) 272
BveI ACCTGC 1 cut(s) 443
Cac8I GCNNGC 2 cut(s) 25, 650
CciI TCATGA 1 cut(s) 295
Cfr13I GGNCC 2 cut(s) 220, 389
Csp6I GTAC 2 cut(s) 210, 607
CviAII CATG 6 cut(s) 24, 95, 296, 320, 579, 598
CviJI RGCY 6 cut(s) 109, 329, 476, 596, 648, 652
CviKI_1 RGCY 6 cut(s) 109, 329, 476, 596, 648, 652
CviQI GTAC 2 cut(s) 210, 607
DdeI CTNAG 3 cut(s) 264, 419, 653
DpnI GATC 4 cut(s) 55, 65, 162, 480
DpnII GATC 4 cut(s) 53, 63, 160, 478
EaeI YGGCCR 1 cut(s) 594
Eco47I GGWCC 2 cut(s) 220, 389
EcoRII CCWGG 1 cut(s) 222
FaeI CATG 6 cut(s) 27, 98, 299, 323, 582, 601
FalI AAGNNNNNCTT 2 cut(s) 105, 137
FatI CATG 6 cut(s) 23, 94, 295, 319, 578, 597
FauNDI CATATG 1 cut(s) 468
FblI GTMKAC 1 cut(s) 118
Fnu4HI GCNGC 1 cut(s) 439
FokI GGATG 2 cut(s) 17, 214
Fsp4HI GCNGC 1 cut(s) 439
FspBI CTAG 1 cut(s) 330
GluI GCNGC 1 cut(s) 439
HaeIII GGCC 1 cut(s) 596
Hin1II CATG 6 cut(s) 27, 98, 299, 323, 582, 601
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 2 cut(s) 282, 512
HpaI GTTAAC 1 cut(s) 631
Hpy166II GTNNAC 3 cut(s) 119, 212, 631
Hpy188I TCNGA 1 cut(s) 517
Hpy188III TCNNGA 1 cut(s) 296
Hpy8I GTNNAC 3 cut(s) 119, 212, 631
HpyAV CCTTC 1 cut(s) 456
HpyCH4III ACNGT 1 cut(s) 239
HpyCH4V TGCA 4 cut(s) 235, 441, 578, 623
HpyF3I CTNAG 3 cut(s) 264, 419, 653
Hsp92II CATG 6 cut(s) 27, 98, 299, 323, 582, 601
KspAI GTTAAC 1 cut(s) 631
Kzo9I GATC 4 cut(s) 53, 63, 160, 478
LpnPI CCDG 7 cut(s) 147, 171, 209, 236, 448, 634, 699
Lsp1109I GCAGC 1 cut(s) 425
LweI GCATC 2 cut(s) 357, 387
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 2 cut(s) 187, 253
MalI GATC 4 cut(s) 55, 65, 162, 480
MboI GATC 4 cut(s) 53, 63, 160, 478
MboII GAAGA 2 cut(s) 291, 548
MflI RGATCY 1 cut(s) 160
MlsI TGGCCA 1 cut(s) 596
MluCI AATT 5 cut(s) 82, 288, 405, 423, 659
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 1 cut(s) 276
MnlI CCTC 4 cut(s) 175, 292, 380, 637
Mox20I TGGCCA 1 cut(s) 596
MroXI GAANNNNTTC 1 cut(s) 141
MscI TGGCCA 1 cut(s) 596
MseI TTAA 7 cut(s) 36, 104, 291, 363, 383, 408, 630
MslI CAYNNNNRTG 2 cut(s) 568, 602
Msp20I TGGCCA 1 cut(s) 596
MspA1I CMGCKG 1 cut(s) 476
MspCI CTTAAG 2 cut(s) 362, 382
MspR9I CCNGG 1 cut(s) 224
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 1 cut(s) 224
NdeI CATATG 1 cut(s) 468
NdeII GATC 4 cut(s) 53, 63, 160, 478
NlaIII CATG 6 cut(s) 27, 98, 299, 323, 582, 601
NspI RCATGY 2 cut(s) 27, 323
PaeI GCATGC 1 cut(s) 27
PagI TCATGA 1 cut(s) 295
PctI GAATGC 1 cut(s) 625
PdmI GAANNNNTTC 1 cut(s) 141
PfeI GAWTC 1 cut(s) 512
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 1 cut(s) 440
Ple19I CGATCG 1 cut(s) 56
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
PshBI ATTAAT 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 222
PspGI CCWGG 1 cut(s) 222
PspPI GGNCC 2 cut(s) 220, 389
PsuI RGATCY 1 cut(s) 160
PvuI CGATCG 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 2 cut(s) 211, 608
RsaNI GTAC 2 cut(s) 210, 607
RseI CAYNNNNRTG 2 cut(s) 568, 602
SaqAI TTAA 7 cut(s) 36, 104, 291, 363, 383, 408, 630
SatI GCNGC 1 cut(s) 439
Sau3AI GATC 4 cut(s) 53, 63, 160, 478
Sau96I GGNCC 2 cut(s) 220, 389
SchI GAGTC 1 cut(s) 276
ScrFI CCNGG 1 cut(s) 224
SetI ASST 8 cut(s) 77, 118, 197, 233, 331, 391, 437, 478
SfaNI GCATC 2 cut(s) 357, 387
SinI GGWCC 2 cut(s) 220, 389
SmiMI CAYNNNNRTG 2 cut(s) 568, 602
SmlI CTYRAG 3 cut(s) 324, 362, 382
SmoI CTYRAG 3 cut(s) 324, 362, 382
SphI GCATGC 1 cut(s) 27
Sse9I AATT 5 cut(s) 82, 288, 405, 423, 659
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 239
TaqI TCGA 1 cut(s) 52
TaqII GACCGA 1 cut(s) 720
TasI AATT 5 cut(s) 82, 288, 405, 423, 659
TatI WGTACW 1 cut(s) 606
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 7 cut(s) 36, 104, 291, 363, 383, 408, 630
Tru9I TTAA 7 cut(s) 36, 104, 291, 363, 383, 408, 630
TscAI CASTG 1 cut(s) 272
TseI GCWGC 1 cut(s) 438
TspDTI ATGAA 6 cut(s) 19, 21, 329, 507, 549, 614
TspRI CASTG 1 cut(s) 272
Vha464I CTTAAG 2 cut(s) 362, 382
VpaK11BI GGWCC 2 cut(s) 220, 389
VspI ATTAAT 1 cut(s) 291
XceI RCATGY 2 cut(s) 27, 323
XcmI CCANNNNNNNNNTGG 1 cut(s) 165
XmiI GTMKAC 1 cut(s) 118
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.