Rmu_sc0000288.1_g000044
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000288.1
OG0000468
Physical Location & Seq
Forward (+)
154831 .. 156003
1173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000288.1_g000044.1.cds

Sequence Viewer

Length: 711 bp
atgtatatcattgctgaacgacattatcgtaagtaccgattgaaatcaaaatctatctacgatgctttttcaagagttggaaattttcaacgcatggtgtatgtgagtgatgtcaactgcataaatcagctccggatggataggtatacatttaacaaattgtgctccatgatacgcacatctggtagattgcgagatactagaactgtttctgttgatgaaatggtagctatgttcttgaacatacttgcacaccataaaaagaacaaaatcatacaagacaagtttttacgttcaggggaaactgttagcagatacttcaatgcagttttagaggcagtcttatgtatacaaggacaattactaaaatcaccagaaccagtggctgagaactcaactgatgagaggtggaagttttttaagaattgtcttggagcattaaatgggacatacattagagtacatgttccagaagttgacaagcctaggtatcgaactaggaataatgaaattgctactaatgtcttggagtatgctctcaagatatgcaattcatttatgtattaccgggttgggagcatgggagtagggagaggggggcgagaaggctacgccactggttgggagggttccactgtagattcaagagtgcttcgtgatgccattagtcggagacatggtttgagagtcccacatggtgagatgaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

236

Amino Acids

27.41

Weight (kDa)

9.86

Isoelectric Point (pI)

31.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 621
AccI GTMKAC 2 cut(s) 146, 349
AccIII TCCGGA 1 cut(s) 132
AcsI RAATTY 1 cut(s) 82
AdeI CACNNNGTG 1 cut(s) 698
AfaI GTAC 2 cut(s) 35, 462
AfiI CCNNNNNNNGG 2 cut(s) 621, 669
AflIII ACRYGT 1 cut(s) 463
AgsI TTSAA 6 cut(s) 43, 72, 89, 241, 322, 645
AluBI AGCT 2 cut(s) 130, 230
AluI AGCT 2 cut(s) 130, 230
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 1 cut(s) 667
AlwNI CAGNNNCTG 1 cut(s) 386
Aor13HI TCCGGA 1 cut(s) 132
ApoI RAATTY 1 cut(s) 82
Asp700I GAANNNNTTC 1 cut(s) 208
AspA2I CCTAGG 1 cut(s) 485
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 2 cut(s) 363, 710
AvrII CCTAGG 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 167
BccI CCATC 1 cut(s) 130
BcnI CCSGG 1 cut(s) 569
BcoDI GTCTC 1 cut(s) 667
BfaI CTAG 3 cut(s) 201, 486, 498
BfmI CTRYAG 1 cut(s) 636
BlnI CCTAGG 1 cut(s) 485
Bme1390I CCNGG 1 cut(s) 569
BmiI GGNNCC 1 cut(s) 631
BmrFI CCNGG 1 cut(s) 569
BmsI GCATC 2 cut(s) 52, 649
BpuEI CTTGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 569
BsaBI GATNNNNATC 1 cut(s) 43
BsaJI CCNNGG 1 cut(s) 485
BsaWI WCCGGW 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 621, 669
Bse1I ACTGG 2 cut(s) 380, 622
Bse3DI GCAATG 1 cut(s) 9
Bse8I GATNNNNATC 1 cut(s) 43
BseAI TCCGGA 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 485
BseGI GGATG 1 cut(s) 141
BseJI GATNNNNATC 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 621, 669
BseMI GCAATG 1 cut(s) 9
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 2 cut(s) 380, 622
BsiHKAI GWGCWC 1 cut(s) 167
BsiSI CCGG 2 cut(s) 133, 568
BslFI GGGAC 2 cut(s) 460, 674
BslI CCNNNNNNNGG 2 cut(s) 621, 669
BsmAI GTCTC 1 cut(s) 667
BsmFI GGGAC 2 cut(s) 460, 674
Bsp1286I GDGCHC 1 cut(s) 167
Bsp13I TCCGGA 1 cut(s) 132
BspCNI CTCAG 1 cut(s) 379
BspEI TCCGGA 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 631
BsrDI GCAATG 1 cut(s) 9
BsrI ACTGG 2 cut(s) 380, 622
BssECI CCNNGG 1 cut(s) 485
BssNAI GTATAC 2 cut(s) 147, 350
BssT1I CCWWGG 1 cut(s) 485
Bst1107I GTATAC 2 cut(s) 147, 350
Bst4CI ACNGT 3 cut(s) 208, 307, 637
BstDEI CTNAG 1 cut(s) 387
BstF5I GGATG 1 cut(s) 141
BstMAI GTCTC 1 cut(s) 667
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 567
BstSFI CTRYAG 1 cut(s) 636
BstZ17I GTATAC 2 cut(s) 147, 350
BtsCI GGATG 1 cut(s) 141
BtsIMutI CAGTG 3 cut(s) 387, 615, 633
CaiI CAGNNNCTG 1 cut(s) 386
Csp6I GTAC 2 cut(s) 34, 461
CviAII CATG 6 cut(s) 94, 169, 464, 580, 677, 695
CviJI RGCY 5 cut(s) 130, 230, 386, 484, 609
CviKI_1 RGCY 5 cut(s) 130, 230, 386, 484, 609
CviQI GTAC 2 cut(s) 34, 461
DdeI CTNAG 1 cut(s) 387
DraIII CACNNNGTG 1 cut(s) 698
Eco130I CCWWGG 1 cut(s) 485
EcoT14I CCWWGG 1 cut(s) 485
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 6 cut(s) 97, 172, 467, 583, 680, 698
FaqI GGGAC 2 cut(s) 460, 674
FatI CATG 6 cut(s) 93, 168, 463, 579, 676, 694
FblI GTMKAC 2 cut(s) 146, 349
FokI GGATG 1 cut(s) 148
FspBI CTAG 3 cut(s) 201, 486, 498
HapII CCGG 2 cut(s) 133, 568
Hin1II CATG 6 cut(s) 97, 172, 467, 583, 680, 698
HincII GTYRAC 2 cut(s) 115, 478
HindII GTYRAC 2 cut(s) 115, 478
HinfI GANTC 2 cut(s) 641, 687
HpaII CCGG 2 cut(s) 133, 568
HphI GGTGA 2 cut(s) 363, 710
Hpy166II GTNNAC 4 cut(s) 115, 147, 350, 478
Hpy188I TCNGA 1 cut(s) 672
Hpy188III TCNNGA 7 cut(s) 72, 133, 238, 470, 541, 645, 656
Hpy8I GTNNAC 4 cut(s) 115, 147, 350, 478
HpyAV CCTTC 1 cut(s) 599
HpyCH4III ACNGT 3 cut(s) 208, 307, 637
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 4 cut(s) 120, 251, 326, 549
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 6 cut(s) 97, 172, 467, 583, 680, 698
Kpn2I TCCGGA 1 cut(s) 132
LmnI GCTCC 4 cut(s) 135, 170, 434, 576
LpnPI CCDG 8 cut(s) 146, 168, 282, 387, 393, 483, 581, 603
LweI GCATC 2 cut(s) 52, 649
MaeI CTAG 3 cut(s) 201, 486, 498
MaeII ACGT 1 cut(s) 292
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 6 cut(s) 82, 158, 359, 424, 510, 550
MlyI GAGTC 1 cut(s) 696
MmeI TCCRAC 2 cut(s) 58, 650
MnlI CCTC 4 cut(s) 328, 399, 587, 619
MroI TCCGGA 1 cut(s) 132
MroXI GAANNNNTTC 1 cut(s) 208
MseI TTAA 3 cut(s) 153, 420, 440
MspI CCGG 2 cut(s) 133, 568
MspR9I CCNGG 1 cut(s) 569
NciI CCSGG 1 cut(s) 569
NlaIII CATG 6 cut(s) 97, 172, 467, 583, 680, 698
NlaIV GGNNCC 1 cut(s) 631
NspI RCATGY 1 cut(s) 467
PciI ACATGT 1 cut(s) 463
PcsI WCGNNNNNNNCGW 2 cut(s) 25, 34
PdmI GAANNNNTTC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 641
PflMI CCANNNNNTGG 1 cut(s) 621
PleI GAGTC 1 cut(s) 695
PpsI GAGTC 1 cut(s) 695
PscI ACATGT 1 cut(s) 463
PspN4I GGNNCC 1 cut(s) 631
PstNI CAGNNNCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 35, 462
RsaNI GTAC 2 cut(s) 34, 461
SaqAI TTAA 3 cut(s) 153, 420, 440
SchI GAGTC 1 cut(s) 696
ScrFI CCNGG 1 cut(s) 569
SduI GDGCHC 1 cut(s) 167
SetI ASST 6 cut(s) 132, 146, 232, 295, 410, 491
SfaNI GCATC 2 cut(s) 52, 649
SfcI CTRYAG 1 cut(s) 636
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 6 cut(s) 82, 158, 359, 424, 510, 550
SspMI CTAG 3 cut(s) 201, 486, 498
StyD4I CCNGG 1 cut(s) 567
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 3 cut(s) 208, 307, 637
TaiI ACGT 1 cut(s) 295
TaqI TCGA 1 cut(s) 493
TasI AATT 6 cut(s) 82, 158, 359, 424, 510, 550
TatI WGTACW 1 cut(s) 460
TfiI GAWTC 1 cut(s) 641
Tru1I TTAA 3 cut(s) 153, 420, 440
Tru9I TTAA 3 cut(s) 153, 420, 440
TscAI CASTG 3 cut(s) 387, 622, 640
TspDTI ATGAA 3 cut(s) 234, 522, 543
TspRI CASTG 3 cut(s) 387, 622, 640
Van91I CCANNNNNTGG 1 cut(s) 621
XapI RAATTY 1 cut(s) 82
XceI RCATGY 1 cut(s) 467
XmaJI CCTAGG 1 cut(s) 485
XmiI GTMKAC 2 cut(s) 146, 349
XmnI GAANNNNTTC 1 cut(s) 208
XspI CTAG 3 cut(s) 201, 486, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.