Rroxscaffold_1G00059020
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000468
Physical Location & Seq
Forward (+)
81029325 .. 81030365
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059020.1

Sequence Viewer

Length: 387 bp
ATGTTTTTACATATACTTTCACATCATGTGAAGAATCGTACTATTAGAGGTAGATTTTTTCGATCCGGAGAGACTATTAGTAGGTATTTCCATTCTATACTACAAGGTGTTTTACGATTGCAAGGTAGTTTGTTGAGGGTACCTGATCCGGTACTTGTTGACTGCACAGATCGTAGATGGAAGTGCTTTAAGAATTGCTTGGGGGCACTAGATGGAACTTACATTAGAGTGCGTGTACCTGCAATTGACAAGCCAAGATACCGCACAAGAAAGGGTGAAGTTGCTACAAATGTGTTAGCTGCTTGTTCTCGTGATATGCAGTTCATATTTGTCTTACCGGTTGGGAGGGATCGGCATCGGACTCTAGAGTTCTTCACGATGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

128

Amino Acids

15.02

Weight (kDa)

11.01

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 1 - 38 1.4e-09 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 247
Acc65I GGTACC 1 cut(s) 139
AccB1I GGYRCC 1 cut(s) 139
AccIII TCCGGA 1 cut(s) 65
AciI CCGC 2 cut(s) 262, 382
AclWI GGATC 3 cut(s) 57, 140, 357
AfaI GTAC 4 cut(s) 40, 141, 153, 237
AgeI ACCGGT 1 cut(s) 337
AluBI AGCT 1 cut(s) 299
AluI AGCT 1 cut(s) 299
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 3 cut(s) 57, 140, 357
Aor13HI TCCGGA 1 cut(s) 65
ApeKI GCWGC 1 cut(s) 299
AsiGI ACCGGT 1 cut(s) 337
Asp718I GGTACC 1 cut(s) 139
AsuHPI GGTGA 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 208
BanI GGYRCC 1 cut(s) 139
BauI CACGAG 1 cut(s) 309
BbvI GCAGC 1 cut(s) 286
BccI CCATC 2 cut(s) 171, 206
BcoDI GTCTC 1 cut(s) 65
BfaI CTAG 2 cut(s) 209, 365
BfuAI ACCTGC 1 cut(s) 247
BisI GCNGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 301
BmiI GGNNCC 1 cut(s) 141
BmsI GCATC 2 cut(s) 364, 369
BsaBI GATNNNNATC 1 cut(s) 354
BsaWI WCCGGW 3 cut(s) 65, 148, 337
Bse118I RCCGGY 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 354
BseAI TCCGGA 1 cut(s) 65
BseJI GATNNNNATC 1 cut(s) 354
BseSI GKGCMC 1 cut(s) 208
BseXI GCAGC 1 cut(s) 286
BsgI GTGCAG 1 cut(s) 148
BshNI GGYRCC 1 cut(s) 139
BshTI ACCGGT 1 cut(s) 337
BsiSI CCGG 3 cut(s) 66, 149, 338
BsmAI GTCTC 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 208
Bsp13I TCCGGA 1 cut(s) 65
Bsp143I GATC 4 cut(s) 62, 145, 169, 349
BspACI CCGC 2 cut(s) 262, 382
BspEI TCCGGA 1 cut(s) 65
BspLI GGNNCC 1 cut(s) 141
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 3 cut(s) 57, 140, 357
BspT107I GGYRCC 1 cut(s) 139
BsrFI RCCGGY 1 cut(s) 337
BssAI RCCGGY 1 cut(s) 337
BssMI GATC 4 cut(s) 62, 145, 169, 349
BssSI CACGAG 1 cut(s) 309
Bst2BI CACGAG 1 cut(s) 309
BstKTI GATC 4 cut(s) 65, 148, 172, 352
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 4 cut(s) 62, 145, 169, 349
BstSLI GKGCMC 1 cut(s) 208
BstV1I GCAGC 1 cut(s) 286
BveI ACCTGC 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 337
Csp6I GTAC 4 cut(s) 39, 140, 152, 236
CspAI ACCGGT 1 cut(s) 337
CviAII CATG 1 cut(s) 26
CviJI RGCY 2 cut(s) 253, 299
CviKI_1 RGCY 2 cut(s) 253, 299
CviQI GTAC 4 cut(s) 39, 140, 152, 236
DpnI GATC 4 cut(s) 64, 147, 171, 351
DpnII GATC 4 cut(s) 62, 145, 169, 349
FaeI CATG 1 cut(s) 29
FaiI YATR 6 cut(s) 12, 14, 27, 98, 317, 326
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FatI CATG 1 cut(s) 25
Fnu4HI GCNGC 1 cut(s) 300
Fsp4HI GCNGC 1 cut(s) 300
FspBI CTAG 2 cut(s) 209, 365
GluI GCNGC 1 cut(s) 300
HapII CCGG 3 cut(s) 66, 149, 338
Hin1II CATG 1 cut(s) 29
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 2 cut(s) 34, 361
HpaII CCGG 3 cut(s) 66, 149, 338
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 2 cut(s) 160, 236
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 4 cut(s) 66, 311, 365, 376
Hpy8I GTNNAC 2 cut(s) 160, 236
HpyCH4V TGCA 4 cut(s) 121, 165, 242, 319
Hsp92II CATG 1 cut(s) 29
Kpn2I TCCGGA 1 cut(s) 65
KpnI GGTACC 1 cut(s) 143
Kzo9I GATC 4 cut(s) 62, 145, 169, 349
LpnPI CCDG 5 cut(s) 79, 156, 162, 252, 351
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 2 cut(s) 364, 369
MaeI CTAG 2 cut(s) 209, 365
MalI GATC 4 cut(s) 64, 147, 171, 351
MboI GATC 4 cut(s) 62, 145, 169, 349
MboII GAAGA 2 cut(s) 43, 364
MfeI CAATTG 1 cut(s) 243
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 2 cut(s) 193, 243
MlyI GAGTC 1 cut(s) 355
MnlI CCTC 3 cut(s) 41, 129, 339
MroI TCCGGA 1 cut(s) 65
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 227
MspI CCGG 3 cut(s) 66, 149, 338
MunI CAATTG 1 cut(s) 243
NdeII GATC 4 cut(s) 62, 145, 169, 349
NlaIII CATG 1 cut(s) 29
NlaIV GGNNCC 1 cut(s) 141
PfeI GAWTC 1 cut(s) 34
PinAI ACCGGT 1 cut(s) 337
PkrI GCNGC 1 cut(s) 301
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
PspN4I GGNNCC 1 cut(s) 141
RsaI GTAC 4 cut(s) 40, 141, 153, 237
RsaNI GTAC 4 cut(s) 39, 140, 152, 236
RseI CAYNNNNRTG 1 cut(s) 227
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 1 cut(s) 300
Sau3AI GATC 4 cut(s) 62, 145, 169, 349
SchI GAGTC 1 cut(s) 355
SduI GDGCHC 1 cut(s) 208
SetI ASST 7 cut(s) 52, 86, 109, 127, 145, 241, 301
SfaNI GCATC 2 cut(s) 364, 369
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 2 cut(s) 193, 243
SsiI CCGC 2 cut(s) 262, 382
SspMI CTAG 2 cut(s) 209, 365
TaqI TCGA 1 cut(s) 61
TasI AATT 2 cut(s) 193, 243
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseI GCWGC 1 cut(s) 299
TspDTI ATGAA 1 cut(s) 313
XbaI TCTAGA 1 cut(s) 364
XspI CTAG 2 cut(s) 209, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.