pycom07g03740
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000468
Physical Location & Seq
Forward (+)
3178714 .. 3182108
3395 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g03740.1

Sequence Viewer

Length: 723 bp
ATGAGCCAGATACCCCTGACAACCCTTAGGCCCTGTTTGGTACACCGTATAAGGCATCGGATAGGACTAATAATACGGTGTACCGTGTTTGGTGACCACCGTGCAAATGCACGGTACAAATTAGTCGGGCTTTGGAAAGAGCAGTCTTCCTCACCATCTCTTCCTCTACAGGCGCTCTCCAGTGCTTTCCAGGTGCTCTTTCTTCCTCTCCAGGCACTCTCTCTTCCTCTCATCCCAGACATGGATCGAAGAAAGATTGTGGCATGCTTCATTGTTAACGTGTGGTACTTGAATATTGTGACTTGGTTGATGATGTGGTTCATCAAGAATCATAATTCAGCTAGAAGGCGGAATCGGCAACTTGCACTTAGAAATCGCACATTTGTTAGGAGAATGGTAGGGTCAGCCTATTTAGATAACATAATAGGATATGATGATGTTCAGTGTGTGAACCAAATTAGAATGGATAGAAGAACGTTCCACTATTTATGTCAAATGCTTCGTGACCATGGTAGAGTAAAATTGGATGGTGTAGTGACAATGGAAGAACAAGTTTGCATGTTTTTGCATATACTTGCACATCATGTCAAGAATCGTACAATTGGGAGTAGGTTTTGTCGATCGGGGGAGACCATAAGTAGGTATTTCAATTCCATTCTACATGGAGTGTTAAGGCTACAAGGTACACTTTTGAAAGTGCCAGAACCCATTCCTAATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

241

Amino Acids

28.03

Weight (kDa)

10.7

Isoelectric Point (pI)

61.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 132 - 223 2.2e-24 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 349
AclI AACGTT 1 cut(s) 476
AclWI GGATC 1 cut(s) 252
AfaI GTAC 6 cut(s) 42, 82, 116, 287, 598, 685
AfiI CCNNNNNNNGG 2 cut(s) 241, 639
AflIII ACRYGT 1 cut(s) 279
AgsI TTSAA 3 cut(s) 292, 649, 694
AjnI CCWGG 2 cut(s) 189, 210
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 623
AlwI GGATC 1 cut(s) 252
AoxI GGCC 1 cut(s) 29
Asp700I GAANNNNTTC 1 cut(s) 708
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 30
AsuHPI GGTGA 2 cut(s) 104, 144
AxyI CCTNAGG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 198
BccI CCATC 2 cut(s) 163, 521
BciT130I CCWGG 2 cut(s) 191, 212
BcoDI GTCTC 1 cut(s) 623
BfaI CTAG 1 cut(s) 342
BfmI CTRYAG 1 cut(s) 167
BfoI RGCGCY 1 cut(s) 176
Bme1390I CCNGG 2 cut(s) 191, 212
BmgT120I GGNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 191, 212
BmsI GCATC 1 cut(s) 64
BpiI GAAGAC 1 cut(s) 138
BpmI CTGGAG 2 cut(s) 163, 194
BsaI GGTCTC 1 cut(s) 623
BsaJI CCNNGG 1 cut(s) 508
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 639
Bse1I ACTGG 1 cut(s) 180
Bse21I CCTNAGG 1 cut(s) 26
BseBI CCWGG 2 cut(s) 191, 212
BseDI CCNNGG 1 cut(s) 508
BseGI GGATG 2 cut(s) 231, 532
BseLI CCNNNNNNNGG 2 cut(s) 241, 639
BseNI ACTGG 1 cut(s) 180
Bsh1285I CGRYCG 1 cut(s) 623
BshFI GGCC 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 623
BsiHKAI GWGCWC 1 cut(s) 198
BslI CCNNNNNNNGG 2 cut(s) 241, 639
BsmAI GTCTC 1 cut(s) 623
BsnI GGCC 1 cut(s) 31
Bso31I GGTCTC 1 cut(s) 623
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 2 cut(s) 244, 620
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 1 cut(s) 349
BspANI GGCC 1 cut(s) 31
BspPI GGATC 1 cut(s) 252
BspTNI GGTCTC 1 cut(s) 623
BsrI ACTGG 1 cut(s) 180
BssECI CCNNGG 1 cut(s) 508
BssMI GATC 2 cut(s) 244, 620
BssT1I CCWWGG 1 cut(s) 508
Bst2UI CCWGG 2 cut(s) 191, 212
Bst4CI ACNGT 5 cut(s) 47, 78, 85, 101, 114
Bst6I CTCTTC 2 cut(s) 165, 228
BstC8I GCNNGC 1 cut(s) 265
BstDEI CTNAG 2 cut(s) 26, 368
BstDSI CCRYGG 1 cut(s) 508
BstEII GGTNACC 1 cut(s) 92
BstF5I GGATG 2 cut(s) 231, 532
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 2 cut(s) 247, 623
BstMAI GTCTC 1 cut(s) 623
BstMBI GATC 2 cut(s) 244, 620
BstMCI CGRYCG 1 cut(s) 623
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNI CCWGG 2 cut(s) 191, 212
BstNSI RCATGY 2 cut(s) 267, 562
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 189, 210
BstSFI CTRYAG 1 cut(s) 167
BstV2I GAAGAC 1 cut(s) 138
Bsu36I CCTNAGG 1 cut(s) 26
BsuRI GGCC 1 cut(s) 31
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 2 cut(s) 231, 532
BtsIMutI CAGTG 2 cut(s) 187, 449
Cac8I GCNNGC 1 cut(s) 265
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 30
Csp6I GTAC 6 cut(s) 41, 81, 115, 286, 597, 684
CviAII CATG 6 cut(s) 241, 264, 509, 559, 584, 662
CviJI RGCY 6 cut(s) 6, 31, 130, 341, 407, 676
CviKI_1 RGCY 6 cut(s) 6, 31, 130, 341, 407, 676
CviQI GTAC 6 cut(s) 41, 81, 115, 286, 597, 684
DdeI CTNAG 2 cut(s) 26, 368
DpnI GATC 2 cut(s) 246, 622
DpnII GATC 2 cut(s) 244, 620
Eam1104I CTCTTC 2 cut(s) 165, 228
EarI CTCTTC 2 cut(s) 165, 228
EciI GGCGGA 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 508
Eco31I GGTCTC 1 cut(s) 623
Eco81I CCTNAGG 1 cut(s) 26
Eco91I GGTNACC 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 30
EcoO65I GGTNACC 1 cut(s) 92
EcoRII CCWGG 2 cut(s) 189, 210
EcoT14I CCWWGG 1 cut(s) 508
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 6 cut(s) 244, 267, 512, 562, 587, 665
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FatI CATG 6 cut(s) 240, 263, 508, 558, 583, 661
FokI GGATG 2 cut(s) 218, 539
FspBI CTAG 1 cut(s) 342
GlaI GCGC 1 cut(s) 174
GsuI CTGGAG 2 cut(s) 163, 194
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 1 cut(s) 31
HhaI GCGC 1 cut(s) 175
Hin1II CATG 6 cut(s) 244, 267, 512, 562, 587, 665
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 3 cut(s) 328, 352, 592
HpaI GTTAAC 1 cut(s) 277
HphI GGTGA 2 cut(s) 104, 144
Hpy166II GTNNAC 5 cut(s) 43, 81, 277, 451, 686
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 3 cut(s) 325, 503, 589
Hpy8I GTNNAC 5 cut(s) 43, 81, 277, 451, 686
HpyAV CCTTC 1 cut(s) 339
HpyCH4III ACNGT 5 cut(s) 47, 78, 85, 101, 114
HpyCH4IV ACGT 2 cut(s) 279, 476
HpyCH4V TGCA 6 cut(s) 104, 110, 365, 558, 568, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 2 cut(s) 26, 368
HpySE526I ACGT 2 cut(s) 279, 476
Hsp92II CATG 6 cut(s) 244, 267, 512, 562, 587, 665
HspAI GCGC 1 cut(s) 173
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 2 cut(s) 244, 620
LweI GCATC 1 cut(s) 64
MaeI CTAG 1 cut(s) 342
MaeII ACGT 2 cut(s) 279, 476
MaeIII GTNAC 4 cut(s) 92, 298, 503, 535
MalI GATC 2 cut(s) 246, 622
MboI GATC 2 cut(s) 244, 620
MboII GAAGA 7 cut(s) 138, 152, 194, 215, 261, 483, 557
MfeI CAATTG 1 cut(s) 600
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 6 cut(s) 119, 334, 456, 521, 600, 649
MnlI CCTC 4 cut(s) 160, 174, 216, 237
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 2 cut(s) 276, 671
MspR9I CCNGG 2 cut(s) 191, 212
MunI CAATTG 1 cut(s) 600
MvaI CCWGG 2 cut(s) 191, 212
MwoI GCNNNNNNNGC 1 cut(s) 355
NcoI CCATGG 1 cut(s) 508
NdeII GATC 2 cut(s) 244, 620
NlaIII CATG 6 cut(s) 244, 267, 512, 562, 587, 665
NmuCI GTSAC 4 cut(s) 92, 298, 503, 535
NspI RCATGY 2 cut(s) 267, 562
PaeI GCATGC 1 cut(s) 267
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 3 cut(s) 328, 352, 592
Ple19I CGATCG 1 cut(s) 623
Psp1406I AACGTT 1 cut(s) 476
Psp6I CCWGG 2 cut(s) 189, 210
PspEI GGTNACC 1 cut(s) 92
PspGI CCWGG 2 cut(s) 189, 210
PspPI GGNCC 1 cut(s) 30
PvuI CGATCG 1 cut(s) 623
RsaI GTAC 6 cut(s) 42, 82, 116, 287, 598, 685
RsaNI GTAC 6 cut(s) 41, 81, 115, 286, 597, 684
SaqAI TTAA 2 cut(s) 276, 671
Sau3AI GATC 2 cut(s) 244, 620
Sau96I GGNCC 1 cut(s) 30
ScrFI CCNGG 2 cut(s) 191, 212
SduI GDGCHC 1 cut(s) 198
SetI ASST 7 cut(s) 195, 282, 343, 479, 614, 644, 685
SfaNI GCATC 1 cut(s) 64
SfcI CTRYAG 1 cut(s) 167
SphI GCATGC 1 cut(s) 267
Sse9I AATT 6 cut(s) 119, 334, 456, 521, 600, 649
SsiI CCGC 1 cut(s) 349
SspI AATATT 1 cut(s) 295
SspMI CTAG 1 cut(s) 342
StyD4I CCNGG 2 cut(s) 189, 210
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 5 cut(s) 47, 78, 85, 101, 114
TaiI ACGT 2 cut(s) 282, 479
TaqI TCGA 2 cut(s) 247, 619
TasI AATT 6 cut(s) 119, 334, 456, 521, 600, 649
TfiI GAWTC 3 cut(s) 328, 352, 592
Tru1I TTAA 2 cut(s) 276, 671
Tru9I TTAA 2 cut(s) 276, 671
TscAI CASTG 2 cut(s) 187, 449
TseFI GTSAC 4 cut(s) 92, 298, 503, 535
Tsp45I GTSAC 4 cut(s) 92, 298, 503, 535
TspDTI ATGAA 2 cut(s) 259, 310
TspRI CASTG 2 cut(s) 187, 449
XceI RCATGY 2 cut(s) 267, 562
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.