FvH4_3g22430

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000704
Physical Location & Seq
Reverse (-)
15483957 .. 15485881
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22430.t1

Sequence Viewer

Length: 1350 bp
ATGTCATCAGGAAGACTTCCGCCGGGATCATTAGGACTACCCCTCATAGGCCAGAGCCTTGTTCTCCTCCGAGCTATGCGAAAGAACACTGCTGAGAAGTGGCTTGAACAAAGGATCAAAAAATATGGTCCAGTTTCGAAGCTGAGTCTCTTCGGCAAGCCAACAGTCTTCATCCATGGACAAGCTGCGAACAAGTTTGTATTCACCAAAGATGGGAGCACCATTTCCAGCCAACAGACTGAATCGATTAAAAGAATTCTGGGGGAGCGGAGTATATTTGAGCTGAGTATCGAGGATCACAAGCGTGTGAGAAGTGCTCTTATGCTGTTCTTGAAGCCTGAGTCACTAAAGCAATATGTGGGGAAAATGGACGAAGAAATCAGGAAGCACCTTGAAATGCATTGGCATGGCAAACAAGTAACGGTCTTGCCCCTGATGAAGAACCTCACGTTCAACATAATATGCTCTCTACTTTTTGGGCTTGAACGAGGAACTCGCAGAGAAAAATTCGTGGAGTGCTTTCAAACAATGATTGAAGGAATGTGGTCAGTGCCAATTAACTTGCCCTTCACGCGTTACAACAGGAGCCTCAAGGCAAGCAAGAGAGTTCAAAACATGCTCAAGGAACTTATACACGAAAAGAGGATACAACTTGAGCAGAAAAAGGCTTCTCCACAACAAGACCTCATCACTTGCTTCCTTAGCATCCGAAATGAAGAGGGTGAAGAAGAATTAACAGAATTGGAGATGGTGCACAATGTCATGGTAGTCATGGTTGCAGGACATGACACTTCATCTGTTCTGATTACGTTCCTTTTGCGGCTTCTAGCTAATGAACCAGTTGTTTATGAAGCACTTCTACAAGAGCAGGAAGAAGTAGCAAGAAGTAAACCGGCAGGAGAGTTTCTAACATGGGAAGACCTTGCCAAAATGAAATACACATGGAGAGTAGCACAGGAAACTCTGAGGATCACTCCTCCTGTCTTTGGTGGCTTCAGGACTGCTATCAAAGATATTGAGTTCGGCGGATTCAACATTCCTAAAGGGTGGCAGATATTCTGGGTTGCTCCAATGACCCACATGGACGATAGCATATTCCCAGAACCGGCAAAGTTTGATCCAAGTAGGTTCGACAAGCAAGCATCAGTTCCACCCTACTGCTTTGTAGCATTCGGAGGGGGACCTAGAATATGTCCAGGATATGAGTTTGCCAGGATTGAAACCCTTATTGCAATGCACCATATAATAACTCAGTTCACCTGGAAGCTATGTGCAGACAATCACTTCAGTAGGGCTCCAATGCCAGTACCTACTGGAGGACTGCCCATTGAAATCAAGCCTAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

450

Amino Acids

51.41

Weight (kDa)

9.2

Isoelectric Point (pI)

60.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 7 - 428 4.1e-76 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 268
AccII CGCG 1 cut(s) 574
AciI CCGC 4 cut(s) 20, 268, 820, 1026
AclWI GGATC 5 cut(s) 34, 122, 303, 977, 1112
AcsI RAATTY 2 cut(s) 255, 506
AcuI CTGAAG 2 cut(s) 979, 1270
AfaI GTAC 1 cut(s) 1308
AfiI CCNNNNNNNGG 5 cut(s) 47, 213, 986, 1105, 1316
AflIII ACRYGT 1 cut(s) 572
AjnI CCWGG 3 cut(s) 1195, 1211, 1259
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 6 cut(s) 74, 142, 185, 283, 830, 1267
AluI AGCT 6 cut(s) 74, 142, 185, 283, 830, 1267
Alw21I GWGCWC 3 cut(s) 221, 319, 756
Alw26I GTCTC 1 cut(s) 152
Alw44I GTGCAC 1 cut(s) 752
AlwI GGATC 5 cut(s) 34, 122, 303, 977, 1112
AoxI GGCC 1 cut(s) 49
ApaLI GTGCAC 1 cut(s) 752
ApeKI GCWGC 1 cut(s) 185
ApoI RAATTY 2 cut(s) 255, 506
Asp700I GAANNNNTTC 1 cut(s) 855
AspS9I GGNCC 2 cut(s) 128, 1181
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 3 cut(s) 196, 734, 1249
AsuII TTCGAA 1 cut(s) 137
AvaII GGWCC 2 cut(s) 128, 1181
BaeGI GKGCMC 1 cut(s) 756
BanII GRGCYC 1 cut(s) 1297
BarI GAAGNNNNNNTAC 2 cut(s) 843, 875
BbsI GAAGAC 3 cut(s) 19, 160, 924
Bbv12I GWGCWC 3 cut(s) 221, 319, 756
BbvI GCAGC 1 cut(s) 172
BccI CCATC 2 cut(s) 206, 742
BciT130I CCWGG 3 cut(s) 1197, 1213, 1261
BciVI GTATCC 1 cut(s) 639
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 3 cut(s) 827, 1185, 1341
BfuI GTATCC 1 cut(s) 639
BisI GCNGC 2 cut(s) 186, 821
BlsI GCNGC 2 cut(s) 187, 822
Bme1390I CCNGG 4 cut(s) 24, 1197, 1213, 1261
Bme18I GGWCC 2 cut(s) 128, 1181
BmgT120I GGNCC 2 cut(s) 128, 1181
BmiI GGNNCC 3 cut(s) 587, 1182, 1296
BmrFI CCNGG 4 cut(s) 24, 1197, 1213, 1261
BmsI GCATC 2 cut(s) 714, 1151
BpiI GAAGAC 3 cut(s) 19, 160, 924
BplI GAGNNNNNCTC 4 cut(s) 301, 333, 958, 990
BpmI CTGGAG 1 cut(s) 1335
Bpu10I CCTNAGC 1 cut(s) 701
Bpu14I TTCGAA 1 cut(s) 137
BpuEI CTTGAG 3 cut(s) 575, 605, 674
BpuMI CCSGG 1 cut(s) 24
Bsa29I ATCGAT 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 175
Bsc4I CCNNNNNNNGG 5 cut(s) 47, 213, 986, 1105, 1316
Bse118I RCCGGY 2 cut(s) 892, 1105
Bse1I ACTGG 4 cut(s) 131, 839, 1304, 1318
Bse3DI GCAATG 1 cut(s) 1239
BseBI CCWGG 3 cut(s) 1197, 1213, 1261
BseCI ATCGAT 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 2 cut(s) 171, 705
BseLI CCNNNNNNNGG 5 cut(s) 47, 213, 986, 1105, 1316
BseMI GCAATG 1 cut(s) 1239
BseMII CTCAG 6 cut(s) 84, 134, 275, 330, 956, 1265
BseNI ACTGG 4 cut(s) 131, 839, 1304, 1318
BseRI GAGGAG 2 cut(s) 56, 966
BseSI GKGCMC 1 cut(s) 756
BseXI GCAGC 1 cut(s) 172
BsgI GTGCAG 1 cut(s) 1293
Bsh1236I CGCG 1 cut(s) 574
BshFI GGCC 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 245
BsiHKAI GWGCWC 3 cut(s) 221, 319, 756
BsiSI CCGG 3 cut(s) 23, 893, 1106
BslFI GGGAC 1 cut(s) 1194
BslI CCNNNNNNNGG 5 cut(s) 47, 213, 986, 1105, 1316
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 1194
BsmI GAATGC 1 cut(s) 1169
BsnI GGCC 1 cut(s) 51
Bsp119I TTCGAA 1 cut(s) 137
Bsp1286I GDGCHC 4 cut(s) 221, 319, 756, 1297
Bsp143I GATC 5 cut(s) 26, 114, 295, 969, 1117
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 4 cut(s) 20, 268, 820, 1026
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 6 cut(s) 85, 135, 276, 331, 957, 1264
BspDI ATCGAT 1 cut(s) 245
BspFNI CGCG 1 cut(s) 574
BspLI GGNNCC 3 cut(s) 587, 1182, 1296
BspPI GGATC 5 cut(s) 34, 122, 303, 977, 1112
BspT104I TTCGAA 1 cut(s) 137
BsrBI CCGCTC 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 1239
BsrFI RCCGGY 2 cut(s) 892, 1105
BsrI ACTGG 4 cut(s) 131, 839, 1304, 1318
BssAI RCCGGY 2 cut(s) 892, 1105
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 5 cut(s) 26, 114, 295, 969, 1117
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 3 cut(s) 1197, 1213, 1261
Bst4CI ACNGT 2 cut(s) 166, 424
Bst6I CTCTTC 2 cut(s) 155, 711
BstBI TTCGAA 1 cut(s) 137
BstC8I GCNNGC 3 cut(s) 158, 598, 1140
BstDEI CTNAG 7 cut(s) 93, 143, 284, 339, 701, 965, 1251
BstDSI CCRYGG 1 cut(s) 175
BstENI CCTNNNNNAGG 1 cut(s) 1314
BstF5I GGATG 2 cut(s) 171, 705
BstFNI CGCG 1 cut(s) 574
BstKTI GATC 5 cut(s) 29, 117, 298, 972, 1120
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 5 cut(s) 26, 114, 295, 969, 1117
BstMWI GCNNNNNNNGC 2 cut(s) 571, 702
BstNI CCWGG 3 cut(s) 1197, 1213, 1261
BstNSI RCATGY 1 cut(s) 619
BstSCI CCNGG 4 cut(s) 22, 1195, 1211, 1259
BstSLI GKGCMC 1 cut(s) 756
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 1 cut(s) 172
BstV2I GAAGAC 3 cut(s) 19, 160, 924
Bsu15I ATCGAT 1 cut(s) 245
BsuI GTATCC 1 cut(s) 639
BsuRI GGCC 1 cut(s) 51
BsuTUI ATCGAT 1 cut(s) 245
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 2 cut(s) 171, 705
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 87, 555
Cac8I GCNNGC 3 cut(s) 158, 598, 1140
Cfr10I RCCGGY 2 cut(s) 892, 1105
Cfr13I GGNCC 2 cut(s) 128, 1181
ClaI ATCGAT 1 cut(s) 245
Csp6I GTAC 1 cut(s) 1307
CviQI GTAC 1 cut(s) 1307
DdeI CTNAG 7 cut(s) 93, 143, 284, 339, 701, 965, 1251
DpnI GATC 5 cut(s) 28, 116, 297, 971, 1119
DpnII GATC 5 cut(s) 26, 114, 295, 969, 1117
Eam1104I CTCTTC 2 cut(s) 155, 711
EarI CTCTTC 2 cut(s) 155, 711
EciI GGCGGA 2 cut(s) 9, 1041
Eco130I CCWWGG 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 1297
Eco47I GGWCC 2 cut(s) 128, 1181
Eco57I CTGAAG 2 cut(s) 979, 1270
EcoNI CCTNNNNNAGG 1 cut(s) 1314
EcoO109I RGGNCCY 1 cut(s) 1181
EcoRI GAATTC 1 cut(s) 255
EcoRII CCWGG 3 cut(s) 1195, 1211, 1259
EcoT14I CCWWGG 1 cut(s) 175
EcoT22I ATGCAT 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 1297
ErhI CCWWGG 1 cut(s) 175
FaqI GGGAC 1 cut(s) 1194
Fnu4HI GCNGC 2 cut(s) 186, 821
FokI GGATG 2 cut(s) 158, 692
FriOI GRGCYC 1 cut(s) 1297
Fsp4HI GCNGC 2 cut(s) 186, 821
FspBI CTAG 3 cut(s) 827, 1185, 1341
GluI GCNGC 2 cut(s) 186, 821
GsuI CTGGAG 1 cut(s) 1335
HaeIII GGCC 1 cut(s) 51
HapII CCGG 3 cut(s) 23, 893, 1106
HinfI GANTC 4 cut(s) 145, 242, 341, 1029
HpaII CCGG 3 cut(s) 23, 893, 1106
HphI GGTGA 3 cut(s) 196, 734, 1249
Hpy166II GTNNAC 3 cut(s) 754, 890, 1257
Hpy188I TCNGA 5 cut(s) 71, 710, 804, 966, 1175
Hpy188III TCNNGA 4 cut(s) 9, 331, 382, 997
Hpy8I GTNNAC 3 cut(s) 754, 890, 1257
HpyAV CCTTC 2 cut(s) 530, 577
HpyCH4III ACNGT 2 cut(s) 166, 424
HpyCH4IV ACGT 2 cut(s) 449, 809
HpyCH4V TGCA 6 cut(s) 400, 754, 779, 1232, 1237, 1274
HpyF10VI GCNNNNNNNGC 2 cut(s) 571, 702
HpyF3I CTNAG 7 cut(s) 93, 143, 284, 339, 701, 965, 1251
HpySE526I ACGT 2 cut(s) 449, 809
Kzo9I GATC 5 cut(s) 26, 114, 295, 969, 1117
LmnI GCTCC 5 cut(s) 216, 265, 585, 1072, 1300
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 2 cut(s) 714, 1151
MaeI CTAG 3 cut(s) 827, 1185, 1341
MaeII ACGT 2 cut(s) 449, 809
MaeIII GTNAC 3 cut(s) 342, 418, 575
MalI GATC 5 cut(s) 28, 116, 297, 971, 1119
MbiI CCGCTC 1 cut(s) 268
MboI GATC 5 cut(s) 26, 114, 295, 969, 1117
MhlI GDGCHC 4 cut(s) 221, 319, 756, 1297
MluCI AATT 5 cut(s) 255, 506, 555, 731, 740
MluI ACGCGT 1 cut(s) 572
MlyI GAGTC 2 cut(s) 154, 350
Mph1103I ATGCAT 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 855
MseI TTAA 3 cut(s) 249, 558, 734
MslI CAYNNNNRTG 2 cut(s) 303, 405
MspI CCGG 3 cut(s) 23, 893, 1106
MspR9I CCNGG 4 cut(s) 24, 1197, 1213, 1261
Mva1269I GAATGC 1 cut(s) 1169
MvaI CCWGG 3 cut(s) 1197, 1213, 1261
MvnI CGCG 1 cut(s) 574
MwoI GCNNNNNNNGC 2 cut(s) 571, 702
NciI CCSGG 1 cut(s) 24
NcoI CCATGG 1 cut(s) 175
NdeII GATC 5 cut(s) 26, 114, 295, 969, 1117
NlaIV GGNNCC 3 cut(s) 587, 1182, 1296
NmuCI GTSAC 1 cut(s) 342
NsiI ATGCAT 1 cut(s) 402
NspI RCATGY 1 cut(s) 619
NspV TTCGAA 1 cut(s) 137
OliI CACNNNNGTG 1 cut(s) 303
PctI GAATGC 1 cut(s) 1169
PdmI GAANNNNTTC 1 cut(s) 855
PfeI GAWTC 2 cut(s) 242, 1029
PfoI TCCNGGA 1 cut(s) 1195
PkrI GCNGC 2 cut(s) 187, 822
PleI GAGTC 2 cut(s) 153, 349
PpsI GAGTC 2 cut(s) 153, 349
PpuMI RGGWCCY 1 cut(s) 1181
Psp5II RGGWCCY 1 cut(s) 1181
Psp6I CCWGG 3 cut(s) 1195, 1211, 1259
PspGI CCWGG 3 cut(s) 1195, 1211, 1259
PspN4I GGNNCC 3 cut(s) 587, 1182, 1296
PspPI GGNCC 2 cut(s) 128, 1181
PspPPI RGGWCCY 1 cut(s) 1181
RsaI GTAC 1 cut(s) 1308
RsaNI GTAC 1 cut(s) 1307
RseI CAYNNNNRTG 2 cut(s) 303, 405
SaqAI TTAA 3 cut(s) 249, 558, 734
SatI GCNGC 2 cut(s) 186, 821
Sau3AI GATC 5 cut(s) 26, 114, 295, 969, 1117
Sau96I GGNCC 2 cut(s) 128, 1181
SchI GAGTC 2 cut(s) 154, 350
ScrFI CCNGG 4 cut(s) 24, 1197, 1213, 1261
SduI GDGCHC 4 cut(s) 221, 319, 756, 1297
SfaNI GCATC 2 cut(s) 714, 1151
SfuI TTCGAA 1 cut(s) 137
SinI GGWCC 2 cut(s) 128, 1181
SmiMI CAYNNNNRTG 2 cut(s) 303, 405
SmlI CTYRAG 3 cut(s) 590, 620, 653
SmoI CTYRAG 3 cut(s) 590, 620, 653
Sse9I AATT 5 cut(s) 255, 506, 555, 731, 740
SsiI CCGC 4 cut(s) 20, 268, 820, 1026
SspMI CTAG 3 cut(s) 827, 1185, 1341
StyD4I CCNGG 4 cut(s) 22, 1195, 1211, 1259
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 2 cut(s) 166, 424
TaiI ACGT 2 cut(s) 452, 812
TaqI TCGA 4 cut(s) 137, 245, 291, 1131
TasI AATT 5 cut(s) 255, 506, 555, 731, 740
TauI GCSGC 1 cut(s) 823
TfiI GAWTC 2 cut(s) 242, 1029
Tru1I TTAA 3 cut(s) 249, 558, 734
Tru9I TTAA 3 cut(s) 249, 558, 734
TscAI CASTG 2 cut(s) 94, 555
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 7 cut(s) 160, 452, 729, 783, 849, 864, 947
TspRI CASTG 2 cut(s) 94, 555
VneI GTGCAC 1 cut(s) 752
VpaK11BI GGWCC 2 cut(s) 128, 1181
XagI CCTNNNNNAGG 1 cut(s) 1314
XapI RAATTY 2 cut(s) 255, 506
XceI RCATGY 1 cut(s) 619
XmnI GAANNNNTTC 1 cut(s) 855
XspI CTAG 3 cut(s) 827, 1185, 1341
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.