Rw0G001980

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00062
OG0000704
Physical Location & Seq
Forward (+)
22341 .. 24314
1974 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G001980.1

Sequence Viewer

Length: 1302 bp
ATGATGACTACTCTTTTCACAATTTCCCTCTTCCTCATTCCCATATACCTTCTCCTAAAGAGCAGAAGAAAATCATTAGAAAGGCTTCCACCAGGATCATTAGGACTGCCCCTAATAGGCCAGAGCCTTGATTTTCTCCGACCGATGCGTGCCAACACTGCAGAGAATTGGCTTGAACAAAGGATCAGAAAGTATGGTCCAGTTTCAAAGCTAAGTCTCTTTGGCAAGCCAACACTCTTCATCCATGGACAAGCTGCAAACAAGTTTATATTCACCACTGATGGGAGCACCATTTCAGCCCAACAAACCGAGTCTATTCGTAGGATTTTGGGGGAACAGAGTATATCCGAGTTAAGTGCTGAGGATCACAAACGTGTTATGCCCCTGATGAAGAACCTCACATTTAACATAATATCCTCTCTACTTTTTGGGCTTGAACGAGGAACTCGGAGAGATGAATTTTTGGAGTGTTTTGAAAAGATGATGCAAGGATTGTGGTCAGTGCCGGTCAACTTGCCCTTCACGCGTTACAACAGCAGCCTCAAGGCAGCAAGAGGGTTCAAAAAATGCTCAAAGAACTTATACGCGAAAGGAGGGTACAACTTGATCAAAAAAAGGGCTTCTCCACAACAAGACCTCATCACTTGCTTCCTTAGCATCCAGAATAATGAAGGTAAAGAAGAATTAACAGAGTTGGAGATGGTGCACAATGTCAGGTTAGTCATGGTTGCAGGGCATGACACTTCGGCTATTCTTATTAAGTTCCTTTTGCGGATTCTAGCTAATGAACCAGCAGTGTATGCTGCCCTTCATCAAGAGCAGGAGGAAATAGCTAGAACCAAATTGGCAGGAGAATTTTTAACATGGGAAGATCTTGCCAAAATGAAATACACATGGAGTGTAGCGCTGGAAACTCTGAGAATGATTCCTCCTTCCTTTGGTAGCTTTAGGACAGCTGTCAAAGATATTGAGTTCGGTGGATTCCACATTCCTAAAGGGTGGCAGATATTCTGGGCTACTCCTATGACCCACATGGATGATAGAATATTTCCAGAGCCATCGAAGTTTGATCCAAGTAGGTTCAACAACCAAGCAACAGTTCCACCCTACTGCTTTGTACCATTTGGAGGGGGACCTAGAATATGTCCAGGATTTGAGTTTTCCAGGATCGAAACCCTAATTTCAATGCACCATATGGTAACTCAATTCACCTGGAAGCTTTGTGCAGACAATCACTTCAGTAGGGATCTATTGCCTGTACCTACTCAAGGACTGCCCATTGAAATCAAGCCTCGATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

433

Amino Acids

49.49

Weight (kDa)

9.38

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 125 - 412 5e-57 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 526, 587
AciI CCGC 1 cut(s) 772
AclWI GGATC 6 cut(s) 103, 191, 372, 1064, 1175, 1254
AcsI RAATTY 2 cut(s) 458, 854
AcuI CTGAAG 1 cut(s) 1222
AfaI GTAC 3 cut(s) 599, 1119, 1260
AfeI AGCGCT 1 cut(s) 906
AfiI CCNNNNNNNGG 5 cut(s) 116, 282, 938, 1127, 1268
AflIII ACRYGT 2 cut(s) 373, 524
AgsI TTSAA 8 cut(s) 176, 207, 437, 476, 562, 1084, 1185, 1283
AjnI CCWGG 4 cut(s) 91, 1147, 1163, 1211
AjuI GAANNNNNNNTTGG 2 cut(s) 1083, 1115
AleI CACNNNNGTG 1 cut(s) 372
AluBI AGCT 7 cut(s) 211, 254, 782, 833, 945, 956, 1219
AluI AGCT 7 cut(s) 211, 254, 782, 833, 945, 956, 1219
Alw21I GWGCWC 2 cut(s) 290, 708
Alw26I GTCTC 1 cut(s) 221
Alw44I GTGCAC 1 cut(s) 704
AlwI GGATC 6 cut(s) 103, 191, 372, 1064, 1175, 1254
Aor51HI AGCGCT 1 cut(s) 906
AoxI GGCC 1 cut(s) 118
ApaLI GTGCAC 1 cut(s) 704
ApeKI GCWGC 4 cut(s) 254, 537, 548, 803
ApoI RAATTY 2 cut(s) 458, 854
Asp700I GAANNNNTTC 1 cut(s) 84
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 2 cut(s) 197, 1133
AsuHPI GGTGA 2 cut(s) 265, 1201
AvaII GGWCC 2 cut(s) 197, 1133
BaeGI GKGCMC 1 cut(s) 708
Bbv12I GWGCWC 2 cut(s) 290, 708
BbvCI CCTCAGC 1 cut(s) 360
BbvI GCAGC 4 cut(s) 241, 549, 560, 790
BccI CCATC 3 cut(s) 275, 694, 1066
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 4 cut(s) 93, 1149, 1165, 1213
BclI TGATCA 1 cut(s) 606
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 3 cut(s) 779, 834, 1137
BfmI CTRYAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 908
BglII AGATCT 1 cut(s) 871
BisI GCNGC 4 cut(s) 255, 538, 549, 804
BlsI GCNGC 4 cut(s) 256, 539, 550, 805
Bme1390I CCNGG 4 cut(s) 93, 1149, 1165, 1213
Bme18I GGWCC 2 cut(s) 197, 1133
BmgT120I GGNCC 2 cut(s) 197, 1133
BmiI GGNNCC 1 cut(s) 1134
BmrFI CCNGG 4 cut(s) 93, 1149, 1165, 1213
BmsI GCATC 3 cut(s) 135, 474, 666
BoxI GACNNNNGTC 1 cut(s) 956
Bpu10I CCTNAGC 2 cut(s) 360, 653
BpuEI CTTGAG 2 cut(s) 527, 1251
BsaJI CCNNGG 1 cut(s) 244
Bsc4I CCNNNNNNNGG 5 cut(s) 116, 282, 938, 1127, 1268
Bse118I RCCGGY 1 cut(s) 505
Bse1I ACTGG 1 cut(s) 200
BseBI CCWGG 4 cut(s) 93, 1149, 1165, 1213
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 3 cut(s) 240, 657, 1042
BseLI CCNNNNNNNGG 5 cut(s) 116, 282, 938, 1127, 1268
BseMII CTCAG 2 cut(s) 351, 908
BseNI ACTGG 1 cut(s) 200
BseSI GKGCMC 1 cut(s) 708
BseXI GCAGC 4 cut(s) 241, 549, 560, 790
BsgI GTGCAG 1 cut(s) 1245
Bsh1236I CGCG 2 cut(s) 526, 587
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 1 cut(s) 120
BsiEI CGRYCG 1 cut(s) 143
BsiHKAI GWGCWC 2 cut(s) 290, 708
BsiSI CCGG 1 cut(s) 506
BslFI GGGAC 1 cut(s) 1146
BslI CCNNNNNNNGG 5 cut(s) 116, 282, 938, 1127, 1268
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 1146
BsnI GGCC 1 cut(s) 120
Bsp1286I GDGCHC 2 cut(s) 290, 708
Bsp143I GATC 8 cut(s) 95, 183, 364, 606, 871, 1069, 1167, 1246
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 1 cut(s) 772
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 2 cut(s) 352, 909
BspFNI CGCG 2 cut(s) 526, 587
BspLI GGNNCC 1 cut(s) 1134
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 6 cut(s) 103, 191, 372, 1064, 1175, 1254
BsrFI RCCGGY 1 cut(s) 505
BsrI ACTGG 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 505
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 8 cut(s) 95, 183, 364, 606, 871, 1069, 1167, 1246
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 4 cut(s) 93, 1149, 1165, 1213
Bst4CI ACNGT 1 cut(s) 1099
Bst6I CTCTTC 2 cut(s) 35, 242
BstAPI GCANNNNNTGC 1 cut(s) 800
BstC8I GCNNGC 2 cut(s) 150, 227
BstDEI CTNAG 4 cut(s) 212, 360, 653, 917
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 1266
BstF5I GGATG 3 cut(s) 240, 657, 1042
BstFNI CGCG 2 cut(s) 526, 587
BstH2I RGCGCY 1 cut(s) 908
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 8 cut(s) 98, 186, 367, 609, 874, 1072, 1170, 1249
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 8 cut(s) 95, 183, 364, 606, 871, 1069, 1167, 1246
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 4 cut(s) 158, 523, 654, 800
BstNI CCWGG 4 cut(s) 93, 1149, 1165, 1213
BstPAI GACNNNNGTC 1 cut(s) 956
BstSCI CCNGG 4 cut(s) 91, 1147, 1163, 1211
BstSFI CTRYAG 1 cut(s) 159
BstSLI GKGCMC 1 cut(s) 708
BstUI CGCG 2 cut(s) 526, 587
BstV1I GCAGC 4 cut(s) 241, 549, 560, 790
BstX2I RGATCY 2 cut(s) 871, 1246
BstYI RGATCY 2 cut(s) 871, 1246
BsuRI GGCC 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 240, 657, 1042
BtsI GCAGTG 2 cut(s) 156, 801
BtsIMutI CAGTG 4 cut(s) 156, 276, 507, 801
Cac8I GCNNGC 2 cut(s) 150, 227
CfoI GCGC 1 cut(s) 907
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 2 cut(s) 197, 1133
Csp6I GTAC 3 cut(s) 598, 1118, 1259
CspCI CAANNNNNGTGG 2 cut(s) 476, 511
CviAII CATG 6 cut(s) 245, 724, 737, 864, 894, 1033
CviQI GTAC 3 cut(s) 598, 1118, 1259
DdeI CTNAG 4 cut(s) 212, 360, 653, 917
DpnI GATC 8 cut(s) 97, 185, 366, 608, 873, 1071, 1169, 1248
DpnII GATC 8 cut(s) 95, 183, 364, 606, 871, 1069, 1167, 1246
Eam1104I CTCTTC 2 cut(s) 35, 242
EarI CTCTTC 2 cut(s) 35, 242
Eco130I CCWWGG 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 197, 1133
Eco47III AGCGCT 1 cut(s) 906
Eco57I CTGAAG 1 cut(s) 1222
EcoNI CCTNNNNNAGG 1 cut(s) 1266
EcoO109I RGGNCCY 1 cut(s) 1133
EcoRII CCWGG 4 cut(s) 91, 1147, 1163, 1211
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 6 cut(s) 248, 727, 740, 867, 897, 1036
FaqI GGGAC 1 cut(s) 1146
FatI CATG 6 cut(s) 244, 723, 736, 863, 893, 1032
FauNDI CATATG 1 cut(s) 1194
FbaI TGATCA 1 cut(s) 606
Fnu4HI GCNGC 4 cut(s) 255, 538, 549, 804
FokI GGATG 3 cut(s) 227, 644, 1049
Fsp4HI GCNGC 4 cut(s) 255, 538, 549, 804
FspBI CTAG 3 cut(s) 779, 834, 1137
GlaI GCGC 1 cut(s) 906
GluI GCNGC 4 cut(s) 255, 538, 549, 804
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 1 cut(s) 120
HapII CCGG 1 cut(s) 506
HhaI GCGC 1 cut(s) 907
Hin1II CATG 6 cut(s) 248, 727, 740, 867, 897, 1036
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 1 cut(s) 1217
HinfI GANTC 4 cut(s) 311, 775, 925, 981
HpaII CCGG 1 cut(s) 506
HphI GGTGA 2 cut(s) 265, 1201
Hpy166II GTNNAC 2 cut(s) 511, 706
Hpy188I TCNGA 5 cut(s) 140, 188, 349, 450, 918
Hpy188III TCNNGA 3 cut(s) 661, 815, 1052
Hpy8I GTNNAC 2 cut(s) 511, 706
HpyAV CCTTC 5 cut(s) 59, 529, 665, 818, 942
HpyCH4III ACNGT 1 cut(s) 1099
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 7 cut(s) 161, 257, 487, 706, 731, 1189, 1226
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 523, 654, 800
HpyF3I CTNAG 4 cut(s) 212, 360, 653, 917
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 6 cut(s) 248, 727, 740, 867, 897, 1036
HspAI GCGC 1 cut(s) 905
Ksp22I TGATCA 1 cut(s) 606
Kzo9I GATC 8 cut(s) 95, 183, 364, 606, 871, 1069, 1167, 1246
LmnI GCTCC 1 cut(s) 285
Lsp1109I GCAGC 4 cut(s) 241, 549, 560, 790
LweI GCATC 3 cut(s) 135, 474, 666
MaeI CTAG 3 cut(s) 779, 834, 1137
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 2 cut(s) 527, 1198
MalI GATC 8 cut(s) 97, 185, 366, 608, 873, 1071, 1169, 1248
MboI GATC 8 cut(s) 95, 183, 364, 606, 871, 1069, 1167, 1246
MboII GAAGA 6 cut(s) 22, 78, 229, 403, 692, 881
MflI RGATCY 2 cut(s) 871, 1246
MhlI GDGCHC 2 cut(s) 290, 708
MluCI AATT 8 cut(s) 21, 166, 458, 683, 842, 854, 1179, 1205
MluI ACGCGT 1 cut(s) 524
MlyI GAGTC 1 cut(s) 320
MmeI TCCRAC 2 cut(s) 163, 675
MroXI GAANNNNTTC 1 cut(s) 84
MseI TTAA 5 cut(s) 353, 405, 686, 759, 860
MslI CAYNNNNRTG 2 cut(s) 372, 1035
MspA1I CMGCKG 1 cut(s) 956
MspI CCGG 1 cut(s) 506
MspR9I CCNGG 4 cut(s) 93, 1149, 1165, 1213
MvaI CCWGG 4 cut(s) 93, 1149, 1165, 1213
MvnI CGCG 2 cut(s) 526, 587
MwoI GCNNNNNNNGC 4 cut(s) 158, 523, 654, 800
NcoI CCATGG 1 cut(s) 244
NdeI CATATG 1 cut(s) 1194
NdeII GATC 8 cut(s) 95, 183, 364, 606, 871, 1069, 1167, 1246
NlaIII CATG 6 cut(s) 248, 727, 740, 867, 897, 1036
NlaIV GGNNCC 1 cut(s) 1134
OliI CACNNNNGTG 1 cut(s) 372
PdmI GAANNNNTTC 1 cut(s) 84
PfeI GAWTC 3 cut(s) 775, 925, 981
PfoI TCCNGGA 2 cut(s) 1147, 1163
PkrI GCNGC 4 cut(s) 256, 539, 550, 805
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
PpuMI RGGWCCY 1 cut(s) 1133
PshAI GACNNNNGTC 1 cut(s) 956
Psp5II RGGWCCY 1 cut(s) 1133
Psp6I CCWGG 4 cut(s) 91, 1147, 1163, 1211
PspGI CCWGG 4 cut(s) 91, 1147, 1163, 1211
PspN4I GGNNCC 1 cut(s) 1134
PspPI GGNCC 2 cut(s) 197, 1133
PspPPI RGGWCCY 1 cut(s) 1133
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 2 cut(s) 871, 1246
PvuII CAGCTG 1 cut(s) 956
RsaI GTAC 3 cut(s) 599, 1119, 1260
RsaNI GTAC 3 cut(s) 598, 1118, 1259
RseI CAYNNNNRTG 2 cut(s) 372, 1035
SaqAI TTAA 5 cut(s) 353, 405, 686, 759, 860
SatI GCNGC 4 cut(s) 255, 538, 549, 804
Sau3AI GATC 8 cut(s) 95, 183, 364, 606, 871, 1069, 1167, 1246
Sau96I GGNCC 2 cut(s) 197, 1133
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 4 cut(s) 93, 1149, 1165, 1213
SduI GDGCHC 2 cut(s) 290, 708
SfaNI GCATC 3 cut(s) 135, 474, 666
SfcI CTRYAG 1 cut(s) 159
SinI GGWCC 2 cut(s) 197, 1133
SmiMI CAYNNNNRTG 2 cut(s) 372, 1035
SmlI CTYRAG 2 cut(s) 542, 1266
SmoI CTYRAG 2 cut(s) 542, 1266
Sse9I AATT 8 cut(s) 21, 166, 458, 683, 842, 854, 1179, 1205
SsiI CCGC 1 cut(s) 772
SspI AATATT 1 cut(s) 1047
SspMI CTAG 3 cut(s) 779, 834, 1137
StyD4I CCNGG 4 cut(s) 91, 1147, 1163, 1211
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 1 cut(s) 1099
TaiI ACGT 1 cut(s) 376
TaqI TCGA 3 cut(s) 1061, 1170, 1294
TaqII GACCGA 1 cut(s) 157
TasI AATT 8 cut(s) 21, 166, 458, 683, 842, 854, 1179, 1205
TfiI GAWTC 3 cut(s) 775, 925, 981
Tru1I TTAA 5 cut(s) 353, 405, 686, 759, 860
Tru9I TTAA 5 cut(s) 353, 405, 686, 759, 860
TscAI CASTG 4 cut(s) 163, 283, 507, 801
TseI GCWGC 4 cut(s) 254, 537, 548, 803
TspDTI ATGAA 7 cut(s) 229, 404, 471, 684, 800, 801, 899
TspRI CASTG 4 cut(s) 163, 283, 507, 801
VneI GTGCAC 1 cut(s) 704
VpaK11BI GGWCC 2 cut(s) 197, 1133
XagI CCTNNNNNAGG 1 cut(s) 1266
XapI RAATTY 2 cut(s) 458, 854
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 3 cut(s) 779, 834, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.