pycom03g04120

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000704
Physical Location & Seq
Reverse (-)
3229424 .. 3231844
2421 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04120.4

Sequence Viewer

Length: 1437 bp
ATGGCTTTCATGTTAGCAATTGCGATCACTTTGATAATAGCTTTCTTCGTTTCAAAATTACTTCTGTTTAAAGGTCAGACCAAAAATCTCCCAGGAGGGTCTCTAGGGTATCCTTTGCTAGGAGAGTCCCTGAGCTTCCGCCGAGCACAGAAACAAAACCGAGGCCCTCAGTGGTTCGAAGAAAGAGTTTCAAAACATGGCCCAGTTTTCAAAACCTCCTTGATGGGTTCACCAACTGTCGTTGTTACTGGTCAAGCAGGTAACAAGTTTGTTCTTGGTGGTAGAGATGATGTTCTTGCCACCAAGCAACCGCTCGCCCTTGCAAGCATTGCTGGAAAAGAAAATATATTTGAACTTACAGGATCAAGGTACAAAATGGTAAAGGGAGCAATGATGAGCATCTTAAAGCCTGAAATTCTTCAGAAAAACATCAAACCTATGGATGAATTAATCAAGAACATATTGCTAAGAGAAACAAAGAACAAAGACACCATAAAAGGAGTCGCTACCATGAAGAAGCTCACATTTGAATTAGCATCCAACATCCTTACCGGCATCAAGGATGAGCACACGACTAAGGCTTTGTCTGATGATCTCTCCATAGCCTTCAAAGCAATTGCGACGATTCCCTTCAATTTTCCAGGCACAGTTTTCTGGAAAGGTCTAAGAGCCAGATCAAGGATTGTAAGTCGTATTTTGCCAATACTTGAGCAAAAAAGGGAGGAGCTTTCAAAGGGGAAGTTGAGCCCTACAAGCGATCTGTTCACTTGCTTACTAACCCTAAGAGATGAAAACCAAAAACCACTTTCTGATGATATGATTATAGATAATTATGTCACCTTAATCATAGCTAGTCATGACACTTCAGCCATCCTTCTGAGCCTAATGATATGGAAGCTGTCCAGAGACTCTGAAATCTACAACAAAGTTTTAGAGGAACAAATGGACATTTTAAGGAAGAGGGATGACGAAGCAGAAGATGGACTAACATGGTCTGAGATACACAATATGAAATACACATGGAGAGTTGCACAAGAGTTGATGAGGATGGTACCTCCTGTGTTTGGTAGCTTTCGGACAACAGTTAAAGACACTCAATATGGTGGCTATGACATTCCCAAAGGGTGGCTGGTGCTTTGGATGGCACATGGAACTCACATGAACAAAGATGTATTTGATAATCCCAGAGAGTTTGATCCATCACGGTTTGAGAACCCATCGAAACCAATCCCTCCATTCACTTATCTTCCATTTGGAGGAGGTCTCCACACTTGCATAGGGAATGAGTTTGCCAAAATAGAATTACTCATTGTCATCCACAACTTGGTGACAAAGTTTGAGTGGTCACAGCTGCACCCTGAGGAAGCAATAACTCGTAACCCTGGGCCATATCCATCCATGGGTCTCCCAATCAAGATCAAACCAAGAAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

479

Amino Acids

53.99

Weight (kDa)

9.42

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 248
Acc65I GGTACC 1 cut(s) 1051
AccB1I GGYRCC 1 cut(s) 1051
AccB7I CCANNNNNTGG 2 cut(s) 1125, 1324
AccBSI CCGCTC 1 cut(s) 313
AciI CCGC 2 cut(s) 139, 311
AclWI GGATC 2 cut(s) 370, 1190
AcsI RAATTY 1 cut(s) 414
AcuI CTGAAG 2 cut(s) 404, 849
AfaI GTAC 2 cut(s) 371, 1053
AfiI CCNNNNNNNGG 7 cut(s) 119, 678, 1064, 1125, 1256, 1324, 1400
AgsI TTSAA 8 cut(s) 54, 192, 211, 353, 530, 610, 634, 732
AjnI CCWGG 3 cut(s) 91, 640, 1381
AluBI AGCT 9 cut(s) 41, 135, 520, 727, 851, 898, 1071, 1351, 1432
AluI AGCT 9 cut(s) 41, 135, 520, 727, 851, 898, 1071, 1351, 1432
Alw21I GWGCWC 2 cut(s) 148, 570
Alw26I GTCTC 4 cut(s) 105, 900, 1268, 1409
AlwI GGATC 2 cut(s) 370, 1190
AoxI GGCC 3 cut(s) 163, 199, 1385
ApeKI GCWGC 1 cut(s) 1351
ApoI RAATTY 1 cut(s) 414
ArsI GACNNNNNNTTYG 2 cut(s) 565, 597
AseI ATTAAT 1 cut(s) 449
Asp700I GAANNNNTTC 1 cut(s) 417
Asp718I GGTACC 1 cut(s) 1051
AspS9I GGNCC 3 cut(s) 164, 200, 1385
AsuHPI GGTGA 3 cut(s) 222, 829, 1339
AsuII TTCGAA 1 cut(s) 177
AxyI CCTNAGG 1 cut(s) 1359
BanI GGYRCC 1 cut(s) 1051
BanII GRGCYC 1 cut(s) 749
Bbv12I GWGCWC 2 cut(s) 148, 570
BbvI GCAGC 1 cut(s) 1338
BccI CCATC 8 cut(s) 217, 878, 974, 1042, 1135, 1207, 1225, 1402
BciT130I CCWGG 3 cut(s) 93, 642, 1383
BciVI GTATCC 1 cut(s) 120
BcoDI GTCTC 4 cut(s) 105, 900, 1268, 1409
BfaI CTAG 3 cut(s) 104, 119, 852
BfuAI ACCTGC 1 cut(s) 248
BfuI GTATCC 1 cut(s) 120
BisI GCNGC 1 cut(s) 1352
BlsI GCNGC 1 cut(s) 1353
Bme1390I CCNGG 3 cut(s) 93, 642, 1383
BmgT120I GGNCC 3 cut(s) 164, 200, 1385
BmiI GGNNCC 1 cut(s) 1053
BmrFI CCNGG 3 cut(s) 93, 642, 1383
BmrI ACTGGG 1 cut(s) 197
BmsI GCATC 3 cut(s) 408, 545, 564
BmuI ACTGGG 1 cut(s) 197
BplI GAGNNNNNCTC 2 cut(s) 1248, 1280
Bpu10I CCTNAGC 1 cut(s) 131
Bpu14I TTCGAA 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 728
BsaBI GATNNNNATC 1 cut(s) 398
BsaI GGTCTC 3 cut(s) 105, 1268, 1409
BsaJI CCNNGG 5 cut(s) 91, 160, 1381, 1382, 1398
Bsc4I CCNNNNNNNGG 7 cut(s) 119, 678, 1064, 1125, 1256, 1324, 1400
Bse118I RCCGGY 1 cut(s) 551
Bse1I ACTGG 2 cut(s) 203, 253
Bse21I CCTNAGG 1 cut(s) 1359
Bse3DI GCAATG 2 cut(s) 327, 396
Bse8I GATNNNNATC 1 cut(s) 398
BseBI CCWGG 3 cut(s) 93, 642, 1383
BseDI CCNNGG 5 cut(s) 91, 160, 1381, 1382, 1398
BseJI GATNNNNATC 1 cut(s) 398
BseLI CCNNNNNNNGG 7 cut(s) 119, 678, 1064, 1125, 1256, 1324, 1400
BseMI GCAATG 2 cut(s) 327, 396
BseMII CTCAG 5 cut(s) 122, 182, 869, 987, 1350
BseNI ACTGG 2 cut(s) 203, 253
BseRI GAGGAG 2 cut(s) 737, 1272
BseXI GCAGC 1 cut(s) 1338
BsgI GTGCAG 1 cut(s) 1337
BshFI GGCC 3 cut(s) 165, 201, 1387
BshNI GGYRCC 1 cut(s) 1051
BsiHKAI GWGCWC 2 cut(s) 148, 570
BsiSI CCGG 1 cut(s) 552
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 7 cut(s) 119, 678, 1064, 1125, 1256, 1324, 1400
BsmAI GTCTC 4 cut(s) 105, 900, 1268, 1409
BsmFI GGGAC 1 cut(s) 112
BsnI GGCC 3 cut(s) 165, 201, 1387
Bso31I GGTCTC 3 cut(s) 105, 1268, 1409
Bsp119I TTCGAA 1 cut(s) 177
Bsp1286I GDGCHC 3 cut(s) 148, 570, 749
Bsp143I GATC 7 cut(s) 24, 362, 592, 674, 757, 1195, 1416
Bsp19I CCATGG 1 cut(s) 1398
BspACI CCGC 2 cut(s) 139, 311
BspANI GGCC 3 cut(s) 165, 201, 1387
BspCNI CTCAG 5 cut(s) 123, 181, 870, 988, 1351
BspHI TCATGA 1 cut(s) 856
BspLI GGNNCC 1 cut(s) 1053
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 2 cut(s) 370, 1190
BspT104I TTCGAA 1 cut(s) 177
BspT107I GGYRCC 1 cut(s) 1051
BspTNI GGTCTC 3 cut(s) 105, 1268, 1409
BsrBI CCGCTC 1 cut(s) 313
BsrDI GCAATG 2 cut(s) 327, 396
BsrFI RCCGGY 1 cut(s) 551
BsrI ACTGG 2 cut(s) 203, 253
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 5 cut(s) 91, 160, 1381, 1382, 1398
BssMI GATC 7 cut(s) 24, 362, 592, 674, 757, 1195, 1416
BssT1I CCWWGG 1 cut(s) 1398
Bst2UI CCWGG 3 cut(s) 93, 642, 1383
Bst4CI ACNGT 4 cut(s) 238, 649, 1084, 1206
Bst6I CTCTTC 1 cut(s) 953
BstAPI GCANNNNNTGC 1 cut(s) 329
BstBI TTCGAA 1 cut(s) 177
BstC8I GCNNGC 2 cut(s) 315, 325
BstDEI CTNAG 9 cut(s) 131, 168, 467, 576, 665, 782, 878, 996, 1359
BstDSI CCRYGG 1 cut(s) 1398
BstENI CCTNNNNNAGG 1 cut(s) 117
BstKTI GATC 7 cut(s) 27, 365, 595, 677, 760, 1198, 1419
BstMAI GTCTC 4 cut(s) 105, 900, 1268, 1409
BstMBI GATC 7 cut(s) 24, 362, 592, 674, 757, 1195, 1416
BstMWI GCNNNNNNNGC 3 cut(s) 329, 611, 753
BstNI CCWGG 3 cut(s) 93, 642, 1383
BstSCI CCNGG 3 cut(s) 91, 640, 1381
BstV1I GCAGC 1 cut(s) 1338
Bsu36I CCTNAGG 1 cut(s) 1359
BsuI GTATCC 1 cut(s) 120
BsuRI GGCC 3 cut(s) 165, 201, 1387
BtgI CCRYGG 1 cut(s) 1398
BtsIMutI CAGTG 1 cut(s) 176
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 2 cut(s) 315, 325
CciI TCATGA 1 cut(s) 856
Cfr10I RCCGGY 1 cut(s) 551
Cfr13I GGNCC 3 cut(s) 164, 200, 1385
Csp6I GTAC 2 cut(s) 370, 1052
CviAII CATG 9 cut(s) 10, 197, 511, 857, 990, 1020, 1148, 1159, 1399
CviQI GTAC 2 cut(s) 370, 1052
DdeI CTNAG 9 cut(s) 131, 168, 467, 576, 665, 782, 878, 996, 1359
DpnI GATC 7 cut(s) 26, 364, 594, 676, 759, 1197, 1418
DpnII GATC 7 cut(s) 24, 362, 592, 674, 757, 1195, 1416
DraI TTTAAA 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 953
EarI CTCTTC 1 cut(s) 953
EciI GGCGGA 1 cut(s) 128
Eco130I CCWWGG 1 cut(s) 1398
Eco24I GRGCYC 1 cut(s) 749
Eco31I GGTCTC 3 cut(s) 105, 1268, 1409
Eco57I CTGAAG 2 cut(s) 404, 849
Eco81I CCTNAGG 1 cut(s) 1359
EcoNI CCTNNNNNAGG 1 cut(s) 117
EcoO109I RGGNCCY 1 cut(s) 164
EcoRII CCWGG 3 cut(s) 91, 640, 1381
EcoT14I CCWWGG 1 cut(s) 1398
EcoT38I GRGCYC 1 cut(s) 749
ErhI CCWWGG 1 cut(s) 1398
FaeI CATG 9 cut(s) 13, 200, 514, 860, 993, 1023, 1151, 1162, 1402
FaqI GGGAC 1 cut(s) 112
FatI CATG 9 cut(s) 9, 196, 510, 856, 989, 1019, 1147, 1158, 1398
Fnu4HI GCNGC 1 cut(s) 1352
FriOI GRGCYC 1 cut(s) 749
Fsp4HI GCNGC 1 cut(s) 1352
FspBI CTAG 3 cut(s) 104, 119, 852
GluI GCNGC 1 cut(s) 1352
HaeIII GGCC 3 cut(s) 165, 201, 1387
HapII CCGG 1 cut(s) 552
Hin1II CATG 9 cut(s) 13, 200, 514, 860, 993, 1023, 1151, 1162, 1402
HindIII AAGCTT 1 cut(s) 1430
HinfI GANTC 4 cut(s) 125, 501, 625, 908
HpaII CCGG 1 cut(s) 552
HphI GGTGA 3 cut(s) 222, 829, 1339
Hpy166II GTNNAC 2 cut(s) 230, 765
Hpy188I TCNGA 8 cut(s) 78, 423, 589, 811, 879, 913, 997, 1077
Hpy188III TCNNGA 5 cut(s) 454, 655, 857, 903, 1414
Hpy8I GTNNAC 2 cut(s) 230, 765
Hpy99I CGWCG 1 cut(s) 625
HpyAV CCTTC 3 cut(s) 616, 640, 884
HpyCH4III ACNGT 4 cut(s) 238, 649, 1084, 1206
HpyCH4V TGCA 4 cut(s) 323, 1031, 1275, 1354
HpyF10VI GCNNNNNNNGC 3 cut(s) 329, 611, 753
HpyF3I CTNAG 9 cut(s) 131, 168, 467, 576, 665, 782, 878, 996, 1359
Hsp92II CATG 9 cut(s) 13, 200, 514, 860, 993, 1023, 1151, 1162, 1402
KpnI GGTACC 1 cut(s) 1055
Kzo9I GATC 7 cut(s) 24, 362, 592, 674, 757, 1195, 1416
LmnI GCTCC 2 cut(s) 386, 724
Lsp1109I GCAGC 1 cut(s) 1338
LweI GCATC 3 cut(s) 408, 545, 564
MaeI CTAG 3 cut(s) 104, 119, 852
MaeIII GTNAC 6 cut(s) 244, 260, 835, 1327, 1344, 1376
MalI GATC 7 cut(s) 26, 364, 594, 676, 759, 1197, 1418
MbiI CCGCTC 1 cut(s) 313
MboI GATC 7 cut(s) 24, 362, 592, 674, 757, 1195, 1416
MboII GAAGA 7 cut(s) 37, 191, 410, 526, 970, 989, 1238
MfeI CAATTG 2 cut(s) 18, 615
MhlI GDGCHC 3 cut(s) 148, 570, 749
MluCI AATT 9 cut(s) 18, 56, 414, 446, 530, 615, 634, 829, 1301
MlyI GAGTC 3 cut(s) 134, 510, 902
MmeI TCCRAC 1 cut(s) 564
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 6 cut(s) 69, 404, 449, 842, 953, 1086
MspA1I CMGCKG 1 cut(s) 1351
MspI CCGG 1 cut(s) 552
MspR9I CCNGG 3 cut(s) 93, 642, 1383
MunI CAATTG 2 cut(s) 18, 615
MvaI CCWGG 3 cut(s) 93, 642, 1383
MwoI GCNNNNNNNGC 3 cut(s) 329, 611, 753
NcoI CCATGG 1 cut(s) 1398
NdeII GATC 7 cut(s) 24, 362, 592, 674, 757, 1195, 1416
NlaIII CATG 9 cut(s) 13, 200, 514, 860, 993, 1023, 1151, 1162, 1402
NlaIV GGNNCC 1 cut(s) 1053
NmeAIII GCCGAG 1 cut(s) 167
NmuCI GTSAC 3 cut(s) 835, 1327, 1344
NspV TTCGAA 1 cut(s) 177
PagI TCATGA 1 cut(s) 856
PasI CCCWGGG 1 cut(s) 1382
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 1 cut(s) 625
PflMI CCANNNNNTGG 2 cut(s) 1125, 1324
PkrI GCNGC 1 cut(s) 1353
PleI GAGTC 3 cut(s) 133, 509, 902
PpsI GAGTC 3 cut(s) 133, 509, 902
PshBI ATTAAT 1 cut(s) 449
Psp6I CCWGG 3 cut(s) 91, 640, 1381
PspGI CCWGG 3 cut(s) 91, 640, 1381
PspN4I GGNNCC 1 cut(s) 1053
PspPI GGNCC 3 cut(s) 164, 200, 1385
PvuII CAGCTG 1 cut(s) 1351
RsaI GTAC 2 cut(s) 371, 1053
RsaNI GTAC 2 cut(s) 370, 1052
SaqAI TTAA 6 cut(s) 69, 404, 449, 842, 953, 1086
SatI GCNGC 1 cut(s) 1352
Sau3AI GATC 7 cut(s) 24, 362, 592, 674, 757, 1195, 1416
Sau96I GGNCC 3 cut(s) 164, 200, 1385
SchI GAGTC 3 cut(s) 134, 510, 902
ScrFI CCNGG 3 cut(s) 93, 642, 1383
SduI GDGCHC 3 cut(s) 148, 570, 749
SfaNI GCATC 3 cut(s) 408, 545, 564
SfuI TTCGAA 1 cut(s) 177
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
Sse9I AATT 9 cut(s) 18, 56, 414, 446, 530, 615, 634, 829, 1301
SsiI CCGC 2 cut(s) 139, 311
SspMI CTAG 3 cut(s) 104, 119, 852
StyD4I CCNGG 3 cut(s) 91, 640, 1381
StyI CCWWGG 1 cut(s) 1398
TaaI ACNGT 4 cut(s) 238, 649, 1084, 1206
TaqI TCGA 2 cut(s) 177, 1220
TasI AATT 9 cut(s) 18, 56, 414, 446, 530, 615, 634, 829, 1301
TfiI GAWTC 1 cut(s) 625
Tru1I TTAA 6 cut(s) 69, 404, 449, 842, 953, 1086
Tru9I TTAA 6 cut(s) 69, 404, 449, 842, 953, 1086
TscAI CASTG 1 cut(s) 176
TseFI GTSAC 3 cut(s) 835, 1327, 1344
TseI GCWGC 1 cut(s) 1351
Tsp45I GTSAC 3 cut(s) 835, 1327, 1344
TspDTI ATGAA 5 cut(s) 459, 527, 804, 1025, 1175
TspRI CASTG 1 cut(s) 176
Van91I CCANNNNNTGG 2 cut(s) 1125, 1324
VspI ATTAAT 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 117
XapI RAATTY 1 cut(s) 414
XcmI CCANNNNNNNNNTGG 1 cut(s) 1126
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 3 cut(s) 104, 119, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.