pycom03g15490

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000704
Physical Location & Seq
Forward (+)
16732345 .. 16734234
1890 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g15490.1

Sequence Viewer

Length: 1431 bp
ATGGATGCCCTTCTCCTCACAGTTTTCCTCTTTCTCATCCCCGTCTTCTTTCTCCTTACAAGAAGAAAAAAATCATCAGAACGGCTTCCTCCCGGCTCATTGGGACTACCCATTATAGGTCAAAGCCTTGGTTTGCTTAGAGCCATGCGTGCCAACACTGCCGAAAAGTGGCTTGAACGAAGAATCACAAGGTATGGTCCAGTTTCAAAGATGAGTCTCTTTGGCAAGCCAACAGTCTTCATTCATGGACAGGCTGCCAACAAGTTTGTATTCAACAATGATGGCACATCATTTACCAGTCAACAACCTGAGTCTACTCGTATGATTTTGGGCGACCGGAATATACTGGAACTGAGCGGCGAGGATCACAAGCGTGTCAGAAGTGCTCTTATGTTTTTCTTGAAGCCCGAGTCACTGAAGCGATATGTCGGAAAAATGGATGAAGAAATCAGGAATCATCTTGAGATGCACTGGCATGGCAAGCAAAAAATAACAGTTTTGCCACTGATGAAGAACCTCACATTCAACATAATATGCTCTCTACTTTTTGGGGTTGAACGAGGACCTCGCAGAGACGAACTCATTGAGTGTTTCCGACAAATGATAGAAGGACTCTGGTCGGTCCCTGTTAATTTGCCCTTCACGCGTTACAACCAAAGCCTCAAGGCGAGCAAGAAGGTTCAAAAAATGCTCAAGGAACTCGTATGTGAAAAGAGGGTACAACTTGAGCAAAAAACTACTTCTCCACTCCAAGACCTTGTAACTTGTTTGCTTAACATTCGAAACATTAACAACGAAGAAGAATTAACGGAAAATGAGATCCTGCACAATATCATGGTAGTTATGGTCGCAGGATATGACACTTCATCTGTTGTGATTACTTTTCTTTTGCGGCTTCTAGCGAATGAACCTGCTATCCATGCAGCCGTTCTTCAAGAACAAGAAGAAATAGCTAGGAGCAAGTCCTCAGGAGAATTTCTAACATGGGAAGACCTTTCCAAGATGAAGTACACATGGAGAGTGACAATGGAAATTCTGAGGACAACTCCGCCCGTCTTTGGCGGCATGAGGACAGCTATCAAAGATACCGAGTATGGAGGATTCCTCATCCCGAAAGGGTGGCAAGTATTCTGGGCTATTCCTATGACACACAATGACGGTAGTATATTCCCGGAGCCATCAAATTTTGATCCGAGTAGGTTCGAGAACCAAGCATCAGTTCCGCCTTACAGCTTCGTTGCATTTGGAGGAGGATCTCGAATATGTCCAGGATACGAGTTTGCCAGGATTGAAATCCTTGTTTCAATCCATTATATGGTAACTCAGTTCACGTGGAAGCTATGCGCAGACAACAAATTCAGCAGGGTTCCCATGCCGGTACCTACACAAGGACTGCCTATTGAAATCATGCCACGGAAACCAATGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

477

Amino Acids

54.54

Weight (kDa)

9.29

Isoelectric Point (pI)

65.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 30 - 454 4.4e-72 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1345
Acc36I ACCTGC 1 cut(s) 919
Acc65I GGTACC 1 cut(s) 1378
AccB1I GGYRCC 1 cut(s) 1378
AccB7I CCANNNNNTGG 1 cut(s) 1315
AccBSI CCGCTC 1 cut(s) 357
AccI GTMKAC 1 cut(s) 314
AccII CGCG 1 cut(s) 646
AciI CCGC 5 cut(s) 357, 892, 1049, 1062, 1223
AclWI GGATC 4 cut(s) 372, 814, 1184, 1261
AcsI RAATTY 4 cut(s) 974, 1032, 1183, 1355
AcuI CTGAAG 1 cut(s) 437
AcvI CACGTG 1 cut(s) 1332
AfaI GTAC 3 cut(s) 720, 1010, 1380
AfiI CCNNNNNNNGG 5 cut(s) 116, 168, 1058, 1315, 1388
AflIII ACRYGT 1 cut(s) 644
AjnI CCWGG 2 cut(s) 1267, 1283
AjuI GAANNNNNNNTTGG 2 cut(s) 1203, 1235
AleI CACNNNNGTG 1 cut(s) 372
AloI GAACNNNNNNTCC 2 cut(s) 900, 932
AluBI AGCT 4 cut(s) 953, 1076, 1233, 1339
AluI AGCT 4 cut(s) 953, 1076, 1233, 1339
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 2 cut(s) 221, 567
AlwI GGATC 4 cut(s) 372, 814, 1184, 1261
Ama87I CYCGRG 1 cut(s) 407
ApeKI GCWGC 2 cut(s) 254, 923
ApoI RAATTY 4 cut(s) 974, 1032, 1183, 1355
Asp700I GAANNNNTTC 1 cut(s) 84
Asp718I GGTACC 1 cut(s) 1378
AspLEI GCGC 1 cut(s) 1346
AspS9I GGNCC 3 cut(s) 197, 563, 622
AsuC2I CCSGG 2 cut(s) 93, 1172
AsuII TTCGAA 1 cut(s) 781
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 3 cut(s) 197, 563, 622
AxyI CCTNAGG 1 cut(s) 967
BanI GGYRCC 1 cut(s) 1378
BbrPI CACGTG 1 cut(s) 1332
BbsI GAAGAC 3 cut(s) 37, 229, 996
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 2 cut(s) 241, 935
BccI CCATC 2 cut(s) 275, 1186
BceAI ACGGC 2 cut(s) 98, 911
BciT130I CCWGG 2 cut(s) 1269, 1285
BciVI GTATCC 1 cut(s) 1265
BcnI CCSGG 2 cut(s) 93, 1172
BcoDI GTCTC 2 cut(s) 221, 567
BfaI CTAG 2 cut(s) 899, 954
BfuAI ACCTGC 1 cut(s) 919
BfuI GTATCC 1 cut(s) 1265
BisI GCNGC 5 cut(s) 255, 358, 893, 924, 1063
BlsI GCNGC 5 cut(s) 256, 359, 894, 925, 1064
Bme1390I CCNGG 4 cut(s) 93, 1172, 1269, 1285
Bme18I GGWCC 3 cut(s) 197, 563, 622
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 3 cut(s) 197, 563, 622
BmiI GGNNCC 4 cut(s) 624, 1176, 1368, 1380
BmrFI CCNGG 4 cut(s) 93, 1172, 1269, 1285
BmsI GCATC 2 cut(s) 456, 1223
BoxI GACNNNNGTC 1 cut(s) 616
BpiI GAAGAC 3 cut(s) 37, 229, 996
BplI GAGNNNNNCTC 6 cut(s) 564, 596, 1030, 1062, 1089, 1121
Bpu14I TTCGAA 1 cut(s) 781
BpuEI CTTGAG 4 cut(s) 482, 647, 677, 746
BpuMI CCSGG 2 cut(s) 93, 1172
BsaAI YACGTR 1 cut(s) 1332
BsaBI GATNNNNATC 1 cut(s) 1292
BsaJI CCNNGG 2 cut(s) 127, 1412
BsaWI WCCGGW 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
Bsc4I CCNNNNNNNGG 5 cut(s) 116, 168, 1058, 1315, 1388
Bse118I RCCGGY 1 cut(s) 1375
Bse1I ACTGG 4 cut(s) 200, 297, 351, 476
Bse21I CCTNAGG 1 cut(s) 967
Bse8I GATNNNNATC 1 cut(s) 1292
BseBI CCWGG 2 cut(s) 1269, 1285
BseDI CCNNGG 2 cut(s) 127, 1412
BseGI GGATG 4 cut(s) 10, 36, 445, 1107
BseJI GATNNNNATC 1 cut(s) 1292
BseLI CCNNNNNNNGG 5 cut(s) 116, 168, 1058, 1315, 1388
BseMII CTCAG 5 cut(s) 300, 344, 981, 1028, 1337
BseNI ACTGG 4 cut(s) 200, 297, 351, 476
BseRI GAGGAG 2 cut(s) 5, 1263
BseXI GCAGC 2 cut(s) 241, 935
BsgI GTGCAG 1 cut(s) 809
Bsh1236I CGCG 1 cut(s) 646
Bsh1285I CGRYCG 1 cut(s) 337
BshNI GGYRCC 1 cut(s) 1378
BsiEI CGRYCG 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 4 cut(s) 93, 337, 1172, 1376
BslFI GGGAC 2 cut(s) 117, 608
BslI CCNNNNNNNGG 5 cut(s) 116, 168, 1058, 1315, 1388
BsmAI GTCTC 2 cut(s) 221, 567
BsmBI CGTCTC 1 cut(s) 567
BsmFI GGGAC 2 cut(s) 117, 608
BsoBI CYCGRG 1 cut(s) 407
Bsp119I TTCGAA 1 cut(s) 781
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 4 cut(s) 364, 819, 1189, 1253
BspACI CCGC 5 cut(s) 357, 892, 1049, 1062, 1223
BspCNI CTCAG 5 cut(s) 301, 345, 980, 1029, 1336
BspFNI CGCG 1 cut(s) 646
BspLI GGNNCC 4 cut(s) 624, 1176, 1368, 1380
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 4 cut(s) 372, 814, 1184, 1261
BspT104I TTCGAA 1 cut(s) 781
BspT107I GGYRCC 1 cut(s) 1378
BsrBI CCGCTC 1 cut(s) 357
BsrFI RCCGGY 1 cut(s) 1375
BsrI ACTGG 4 cut(s) 200, 297, 351, 476
BssAI RCCGGY 1 cut(s) 1375
BssECI CCNNGG 2 cut(s) 127, 1412
BssMI GATC 4 cut(s) 364, 819, 1189, 1253
BssT1I CCWWGG 1 cut(s) 127
Bst2UI CCWGG 2 cut(s) 1269, 1285
Bst4CI ACNGT 4 cut(s) 22, 235, 496, 1160
BstBAI YACGTR 1 cut(s) 1332
BstBI TTCGAA 1 cut(s) 781
BstC8I GCNNGC 4 cut(s) 150, 227, 482, 670
BstDEI CTNAG 6 cut(s) 137, 309, 353, 967, 1037, 1323
BstDSI CCRYGG 1 cut(s) 1412
BstENI CCTNNNNNAGG 1 cut(s) 1386
BstF5I GGATG 4 cut(s) 10, 36, 445, 1107
BstFNI CGCG 1 cut(s) 646
BstHHI GCGC 1 cut(s) 1346
BstKTI GATC 4 cut(s) 367, 822, 1192, 1256
BstMAI GTCTC 2 cut(s) 221, 567
BstMBI GATC 4 cut(s) 364, 819, 1189, 1253
BstMCI CGRYCG 1 cut(s) 337
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 481, 643, 920
BstNI CCWGG 2 cut(s) 1269, 1285
BstPAI GACNNNNGTC 1 cut(s) 616
BstSCI CCNGG 4 cut(s) 91, 1170, 1267, 1283
BstUI CGCG 1 cut(s) 646
BstV1I GCAGC 2 cut(s) 241, 935
BstV2I GAAGAC 3 cut(s) 37, 229, 996
BstX2I RGATCY 2 cut(s) 819, 1253
BstYI RGATCY 2 cut(s) 819, 1253
Bsu36I CCTNAGG 1 cut(s) 967
BsuI GTATCC 1 cut(s) 1265
BtgI CCRYGG 1 cut(s) 1412
BtsCI GGATG 4 cut(s) 10, 36, 445, 1107
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 4 cut(s) 156, 413, 469, 503
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 4 cut(s) 150, 227, 482, 670
CfoI GCGC 1 cut(s) 1346
Cfr10I RCCGGY 1 cut(s) 1375
Cfr13I GGNCC 3 cut(s) 197, 563, 622
Csp6I GTAC 3 cut(s) 719, 1009, 1379
CviQI GTAC 3 cut(s) 719, 1009, 1379
DdeI CTNAG 6 cut(s) 137, 309, 353, 967, 1037, 1323
DpnI GATC 4 cut(s) 366, 821, 1191, 1255
DpnII GATC 4 cut(s) 364, 819, 1189, 1253
EciI GGCGGA 2 cut(s) 1038, 1212
Eco130I CCWWGG 1 cut(s) 127
Eco47I GGWCC 3 cut(s) 197, 563, 622
Eco57I CTGAAG 1 cut(s) 437
Eco72I CACGTG 1 cut(s) 1332
Eco81I CCTNAGG 1 cut(s) 967
Eco88I CYCGRG 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 1386
EcoO109I RGGNCCY 1 cut(s) 563
EcoRII CCWGG 2 cut(s) 1267, 1283
EcoT14I CCWWGG 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 127
Esp3I CGTCTC 1 cut(s) 567
FaqI GGGAC 2 cut(s) 117, 608
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 5 cut(s) 255, 358, 893, 924, 1063
FokI GGATG 4 cut(s) 17, 23, 452, 1094
Fsp4HI GCNGC 5 cut(s) 255, 358, 893, 924, 1063
FspBI CTAG 2 cut(s) 899, 954
FspI TGCGCA 1 cut(s) 1345
GlaI GCGC 1 cut(s) 1345
GluI GCNGC 5 cut(s) 255, 358, 893, 924, 1063
HapII CCGG 4 cut(s) 93, 337, 1172, 1376
HhaI GCGC 1 cut(s) 1346
Hin6I GCGC 1 cut(s) 1344
HinP1I GCGC 1 cut(s) 1344
HincII GTYRAC 1 cut(s) 302
HindII GTYRAC 1 cut(s) 302
HinfI GANTC 7 cut(s) 183, 214, 311, 410, 454, 612, 1101
HpaII CCGG 4 cut(s) 93, 337, 1172, 1376
Hpy166II GTNNAC 4 cut(s) 302, 315, 1011, 1329
Hpy188I TCNGA 6 cut(s) 79, 380, 431, 596, 1038, 1194
Hpy188III TCNNGA 8 cut(s) 400, 451, 461, 935, 969, 1111, 1204, 1257
Hpy8I GTNNAC 4 cut(s) 302, 315, 1011, 1329
HpyAV CCTTC 4 cut(s) 20, 602, 649, 670
HpyCH4III ACNGT 4 cut(s) 22, 235, 496, 1160
HpyCH4IV ACGT 1 cut(s) 1331
HpyCH4V TGCA 4 cut(s) 469, 826, 923, 1241
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 481, 643, 920
HpyF3I CTNAG 6 cut(s) 137, 309, 353, 967, 1037, 1323
HpySE526I ACGT 1 cut(s) 1331
HspAI GCGC 1 cut(s) 1344
KpnI GGTACC 1 cut(s) 1382
Kzo9I GATC 4 cut(s) 364, 819, 1189, 1253
LmnI GCTCC 2 cut(s) 957, 1174
Lsp1109I GCAGC 2 cut(s) 241, 935
LweI GCATC 2 cut(s) 456, 1223
MaeI CTAG 2 cut(s) 899, 954
MaeII ACGT 1 cut(s) 1331
MaeIII GTNAC 5 cut(s) 411, 647, 760, 1021, 1318
MalI GATC 4 cut(s) 366, 821, 1191, 1255
MbiI CCGCTC 1 cut(s) 357
MboI GATC 4 cut(s) 364, 819, 1189, 1253
MflI RGATCY 2 cut(s) 819, 1253
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 7 cut(s) 631, 803, 974, 1032, 1183, 1355, 1426
MluI ACGCGT 1 cut(s) 644
MlyI GAGTC 4 cut(s) 223, 320, 419, 606
MmeI TCCRAC 2 cut(s) 409, 619
MroXI GAANNNNTTC 1 cut(s) 84
MseI TTAA 5 cut(s) 630, 774, 789, 806, 1429
MslI CAYNNNNRTG 2 cut(s) 372, 474
MspI CCGG 4 cut(s) 93, 337, 1172, 1376
MspR9I CCNGG 4 cut(s) 93, 1172, 1269, 1285
MvaI CCWGG 2 cut(s) 1269, 1285
MvnI CGCG 1 cut(s) 646
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 481, 643, 920
NciI CCSGG 2 cut(s) 93, 1172
NdeII GATC 4 cut(s) 364, 819, 1189, 1253
NlaIV GGNNCC 4 cut(s) 624, 1176, 1368, 1380
NmuCI GTSAC 2 cut(s) 411, 1021
NsbI TGCGCA 1 cut(s) 1345
NspV TTCGAA 1 cut(s) 781
OliI CACNNNNGTG 1 cut(s) 372
PdmI GAANNNNTTC 1 cut(s) 84
PfeI GAWTC 3 cut(s) 183, 454, 1101
PflMI CCANNNNNTGG 1 cut(s) 1315
PfoI TCCNGGA 2 cut(s) 1170, 1267
PkrI GCNGC 5 cut(s) 256, 359, 894, 925, 1064
PleI GAGTC 4 cut(s) 222, 319, 418, 606
PmaCI CACGTG 1 cut(s) 1332
PmlI CACGTG 1 cut(s) 1332
PpsI GAGTC 4 cut(s) 222, 319, 418, 606
Ppu21I YACGTR 1 cut(s) 1332
PpuMI RGGWCCY 1 cut(s) 563
PshAI GACNNNNGTC 1 cut(s) 616
Psp5II RGGWCCY 1 cut(s) 563
Psp6I CCWGG 2 cut(s) 1267, 1283
PspCI CACGTG 1 cut(s) 1332
PspGI CCWGG 2 cut(s) 1267, 1283
PspN4I GGNNCC 4 cut(s) 624, 1176, 1368, 1380
PspPI GGNCC 3 cut(s) 197, 563, 622
PspPPI RGGWCCY 1 cut(s) 563
PsuI RGATCY 2 cut(s) 819, 1253
RsaI GTAC 3 cut(s) 720, 1010, 1380
RsaNI GTAC 3 cut(s) 719, 1009, 1379
RseI CAYNNNNRTG 2 cut(s) 372, 474
SaqAI TTAA 5 cut(s) 630, 774, 789, 806, 1429
SatI GCNGC 5 cut(s) 255, 358, 893, 924, 1063
Sau3AI GATC 4 cut(s) 364, 819, 1189, 1253
Sau96I GGNCC 3 cut(s) 197, 563, 622
SchI GAGTC 4 cut(s) 223, 320, 419, 606
ScrFI CCNGG 4 cut(s) 93, 1172, 1269, 1285
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 2 cut(s) 456, 1223
SfuI TTCGAA 1 cut(s) 781
SinI GGWCC 3 cut(s) 197, 563, 622
SmiMI CAYNNNNRTG 2 cut(s) 372, 474
SmlI CTYRAG 4 cut(s) 461, 662, 692, 725
SmoI CTYRAG 4 cut(s) 461, 662, 692, 725
Sse9I AATT 7 cut(s) 631, 803, 974, 1032, 1183, 1355, 1426
SsiI CCGC 5 cut(s) 357, 892, 1049, 1062, 1223
SspMI CTAG 2 cut(s) 899, 954
StyD4I CCNGG 4 cut(s) 91, 1170, 1267, 1283
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 4 cut(s) 22, 235, 496, 1160
TaiI ACGT 1 cut(s) 1334
TaqI TCGA 3 cut(s) 781, 1203, 1258
TaqII GACCGA 1 cut(s) 610
TasI AATT 7 cut(s) 631, 803, 974, 1032, 1183, 1355, 1426
TatI WGTACW 1 cut(s) 1008
TauI GCSGC 3 cut(s) 360, 895, 1065
TfiI GAWTC 3 cut(s) 183, 454, 1101
Tru1I TTAA 5 cut(s) 630, 774, 789, 806, 1429
Tru9I TTAA 5 cut(s) 630, 774, 789, 806, 1429
TscAI CASTG 4 cut(s) 163, 420, 476, 510
TseFI GTSAC 2 cut(s) 411, 1021
TseI GCWGC 2 cut(s) 254, 923
Tsp45I GTSAC 2 cut(s) 411, 1021
TspDTI ATGAA 7 cut(s) 229, 233, 456, 524, 855, 921, 1019
TspGWI ACGGA 2 cut(s) 824, 1429
TspRI CASTG 4 cut(s) 163, 420, 476, 510
Van91I CCANNNNNTGG 1 cut(s) 1315
VpaK11BI GGWCC 3 cut(s) 197, 563, 622
XagI CCTNNNNNAGG 1 cut(s) 1386
XapI RAATTY 4 cut(s) 974, 1032, 1183, 1355
XmiI GTMKAC 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 2 cut(s) 899, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.