Rorug05G0176600

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000704
Physical Location & Seq
Reverse (-)
16477312 .. 16541073
63762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0176600.1

Sequence Viewer

Length: 1965 bp
ATGTCTTCAATCCCCAGAGACGAAGCAACCTCGATTCAATGGCAGAAGGGCGATTTGATCGGTTCCGGGGCCTTTGGCCGTGTTTACATGGGCATGAACACTGAATCCGGAGAGCTTATTGCTGTGAAAGAGGCTCATACCAAGGTGCTTCAAGATGAAGTGAGGCTTTTAGAAAACCTGTCGCATCCGAAAATTATTAGATGTTTAGGAACCGAGAGGGAGGATGAAACCTTAACTATGCTGTTGGAATATGTACCTGGAGGATCCATACAAGCACTATTGGAGAAATTTGGATCTTTCTCTGTACCTGTAATAAAAAGATTCACCAAGCAGTTGTTGCTGGCACTAGAATATTTACACAAAAAAGGAATCATACATGGGGACATTAAGGGAGCAAATATCCTTGTTGATAATAAAGGGCGCATTAAACTAGCAGATTTTGGTGCATCCAAACAGGCAGCCGTTTCAGGTGCAAAGCCTACAAGGGGTACACCATACTGGATGGCTCCTGAAGTCATTCGTGGGACTGGTCATGACTTCTCTGCAGATATATGGAGCGTTGGATGCACTGTGATTGAGATGGCCACTGGAAATCCTCCTTGGAACCAACAGTTTGAAACGGTAATCACTCTACGTACTGATATCTTCAAATTAATGACAAGCAAGTCTCATCCACCAATCCCTGATCATCTTTCTCCTGCAGCAAAAGATTTTCTGCACGAATGTCTGCAAAAGGAACCAACTGTAAGGCCATCCGCATCTGAGTTGCTTAAGCATCCTTTTTTAACTGGGAAAGAAGTGGAATCTCAGAATTCTGCCGTCATGGAAGTAGAAGTGGCATCCTCATTTTGCCAAACAAAATCTGACGAAATTGATAGCATGTGGGACGATGATGCAAACTCCAATTTTGTATCCAATCCAGCAAGTGGCGGAACTAGAAAATTGATTGTGCAGGGAGCCATTAAACAAGTGTTTTCAAAAATTTTTAAATTCTTTAAGAGGTCGAGAAGAACTACAAGAAACGGACTGGCGAAGGGTGAGTTCAGCATTGCATCTATCAAGGTCGCCACACACAACTTTTCTGAGGAAAATATGATCGGACAAGGGGGATTTGGACCTGTTTATAAGGGAAAATTGGTGACCGGACAAGAAGTAGCTGTGAAAAGCCTTTCATCATCTTCACTTCAAGGAGATAAGGAGTTTAAGAATGAACTGAGACTCATGCAGAAACTCCAACATAGAAACATTATTCAGATATTAGGATTTTGCATTCATGGTGGCGGAAGATTTATAATATACGATGACTTGCTACTAGATTGGAATAAGCGTTTCAACATAATTGAAGGAATTGCTCAAGGATTGGTCTACTTGCACAAATACTCCAGATTGAGAGTAATTCATAGAGATATAAAAGCCAGTAATGTTCTACTGGATGCACAAATGAATCCCAAAATTGCTGATTTTGGTTTAGCAAAAATTTTTGCAGATGATGAAGAGACAGTAAAAATTACGGGAATTTGTGGGACAATGGGTTACATGGCTCCCGAATACCTCATAACGGGCATCCTTTCCATGAAAACCGATGTCTACAGTTATGGAGTGTTAATGCTTGAAATCGTAAGTGGTAACAAAAATTTCAAAGCTGACGGCAATTATTTGGTAAAAGAGGCCGGGTATGCATGGGAGTTGTGGAAAAAAGGTGCAGGGCTAGAACTAATGGATCCAATGCTAGGCAATTCTTGTATTAACAAAGATCAATTCTTAAGATGCTTGCATGTTGGTCTACTATGTGTGGAGGCAAGTGCAGATGATCGGCTAACCATGTCAGATGTGATATCTATGTTGACAATTAAAAGTGTGCAATTACCTCTGCCAAAAAGGCCAGCATATGGGATAGAAAATGATGTGGCTGAAGCTAGTATAAGTGGAAATGAATCAGAAATTTCATCAACAGATGACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

654

Amino Acids

72.32

Weight (kDa)

6.59

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 262 1.3e-69 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 258 2e-41 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 50 - 152 5.4e-07 ABC1 atypical kinase-like domain
Pkinase PF00069 361 - 549 1.3e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 363 - 547 5.8e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1125, 1292
AasI GACNNNNNNGTC 1 cut(s) 664
AccB7I CCANNNNNTGG 2 cut(s) 926, 1889
AccI GTMKAC 3 cut(s) 1365, 1587, 1783
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 3 cut(s) 756, 930, 1281
AclWI GGATC 5 cut(s) 258, 271, 301, 1715, 1728
AcoI YGGCCR 2 cut(s) 76, 582
AcsI RAATTY 8 cut(s) 287, 811, 981, 989, 1476, 1515, 1633, 1941
AcuI CTGAAG 2 cut(s) 531, 1932
AfaI GTAC 4 cut(s) 255, 306, 490, 637
AfiI CCNNNNNNNGG 5 cut(s) 485, 926, 1558, 1730, 1889
AflII CTTAAG 2 cut(s) 770, 1762
AflIII ACRYGT 1 cut(s) 1959
AjnI CCWGG 1 cut(s) 256
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AloI GAACNNNNNNTCC 2 cut(s) 89, 121
AluBI AGCT 4 cut(s) 115, 1157, 1643, 1916
AluI AGCT 4 cut(s) 115, 1157, 1643, 1916
Alw26I GTCTC 4 cut(s) 12, 672, 1210, 1490
AlwI GGATC 5 cut(s) 258, 271, 301, 1715, 1728
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 6 cut(s) 69, 76, 582, 749, 1668, 1880
ApeKI GCWGC 2 cut(s) 458, 701
ApoI RAATTY 8 cut(s) 287, 811, 981, 989, 1476, 1515, 1633, 1941
AseI ATTAAT 1 cut(s) 653
Asp700I GAANNNNTTC 1 cut(s) 516
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 2 cut(s) 69, 1115
AsuC2I CCSGG 2 cut(s) 67, 1672
AsuHPI GGTGA 3 cut(s) 316, 1049, 1150
AvaII GGWCC 1 cut(s) 1115
BalI TGGCCA 1 cut(s) 584
BamHI GGATCC 2 cut(s) 263, 1720
BbvI GCAGC 2 cut(s) 470, 713
BccI CCATC 3 cut(s) 496, 574, 760
BceAI ACGGC 4 cut(s) 63, 446, 803, 1663
BciT130I CCWGG 1 cut(s) 258
BciVI GTATCC 1 cut(s) 922
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 2 cut(s) 67, 1672
BcoDI GTCTC 4 cut(s) 12, 672, 1210, 1490
BfaI CTAG 7 cut(s) 347, 431, 936, 1313, 1709, 1730, 1917
BfmI CTRYAG 3 cut(s) 543, 699, 1588
BfrI CTTAAG 2 cut(s) 770, 1762
BfuI GTATCC 1 cut(s) 922
BglI GCCNNNNNGGC 1 cut(s) 1879
BisI GCNGC 2 cut(s) 459, 702
BlsI GCNGC 2 cut(s) 460, 703
Bme1390I CCNGG 3 cut(s) 67, 258, 1672
Bme18I GGWCC 1 cut(s) 1115
BmgT120I GGNCC 2 cut(s) 69, 1115
BmrFI CCNGG 3 cut(s) 67, 258, 1672
BmrI ACTGGG 1 cut(s) 798
BmuI ACTGGG 1 cut(s) 798
BpmI CTGGAG 2 cut(s) 279, 1366
BpuEI CTTGAG 1 cut(s) 1338
BpuMI CCSGG 2 cut(s) 67, 1672
BsaAI YACGTR 1 cut(s) 635
BsaJI CCNNGG 3 cut(s) 66, 141, 599
BsaWI WCCGGW 2 cut(s) 107, 1142
BsaXI ACNNNNNCTCC 2 cut(s) 1364, 1394
Bsc4I CCNNNNNNNGG 5 cut(s) 485, 926, 1558, 1730, 1889
Bse1I ACTGG 7 cut(s) 503, 532, 592, 793, 1032, 1416, 1434
Bse3DI GCAATG 1 cut(s) 1047
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 66, 141, 599
BseLI CCNNNNNNNGG 5 cut(s) 485, 926, 1558, 1730, 1889
BseMI GCAATG 1 cut(s) 1047
BseMII CTCAG 4 cut(s) 753, 821, 1074, 1205
BseNI ACTGG 7 cut(s) 503, 532, 592, 793, 1032, 1416, 1434
BseXI GCAGC 2 cut(s) 470, 713
BsgI GTGCAG 4 cut(s) 701, 971, 1722, 1824
BshFI GGCC 6 cut(s) 71, 78, 584, 751, 1670, 1882
BsiSI CCGG 4 cut(s) 66, 108, 1143, 1671
BslFI GGGAC 4 cut(s) 395, 538, 899, 1537
BslI CCNNNNNNNGG 5 cut(s) 485, 926, 1558, 1730, 1889
BsmAI GTCTC 4 cut(s) 12, 672, 1210, 1490
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 4 cut(s) 395, 538, 899, 1537
BsmI GAATGC 1 cut(s) 1269
BsnI GGCC 6 cut(s) 71, 78, 584, 751, 1670, 1882
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 8 cut(s) 57, 263, 293, 685, 1095, 1720, 1753, 1810
BspACI CCGC 3 cut(s) 756, 930, 1281
BspANI GGCC 6 cut(s) 71, 78, 584, 751, 1670, 1882
BspCNI CTCAG 4 cut(s) 754, 820, 1075, 1206
BspEI TCCGGA 1 cut(s) 107
BspHI TCATGA 1 cut(s) 532
BspMAI CTGCAG 2 cut(s) 547, 703
BspPI GGATC 5 cut(s) 258, 271, 301, 1715, 1728
BspTI CTTAAG 2 cut(s) 770, 1762
BsrDI GCAATG 1 cut(s) 1047
BsrI ACTGG 7 cut(s) 503, 532, 592, 793, 1032, 1416, 1434
BssECI CCNNGG 3 cut(s) 66, 141, 599
BssMI GATC 8 cut(s) 57, 263, 293, 685, 1095, 1720, 1753, 1810
BssT1I CCWWGG 2 cut(s) 141, 599
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 6 cut(s) 571, 612, 622, 745, 1501, 1592
Bst6I CTCTTC 1 cut(s) 1488
BstAFI CTTAAG 2 cut(s) 770, 1762
BstAPI GCANNNNNTGC 1 cut(s) 337
BstBAI YACGTR 1 cut(s) 635
BstC8I GCNNGC 3 cut(s) 342, 1772, 1884
BstDEI CTNAG 4 cut(s) 762, 807, 1083, 1214
BstEII GGTNACC 1 cut(s) 1138
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 8 cut(s) 60, 266, 296, 688, 1098, 1723, 1756, 1813
BstMAI GTCTC 4 cut(s) 12, 672, 1210, 1490
BstMBI GATC 8 cut(s) 57, 263, 293, 685, 1095, 1720, 1753, 1810
BstMWI GCNNNNNNNGC 4 cut(s) 337, 564, 1676, 1879
BstNI CCWGG 1 cut(s) 258
BstNSI RCATGY 3 cut(s) 883, 1778, 1963
BstPI GGTNACC 1 cut(s) 1138
BstSCI CCNGG 3 cut(s) 65, 256, 1670
BstSFI CTRYAG 3 cut(s) 543, 699, 1588
BstSNI TACGTA 1 cut(s) 635
BstV1I GCAGC 2 cut(s) 470, 713
BstX2I RGATCY 3 cut(s) 263, 293, 1720
BstYI RGATCY 3 cut(s) 263, 293, 1720
BsuI GTATCC 1 cut(s) 922
BsuRI GGCC 6 cut(s) 71, 78, 584, 751, 1670, 1882
BtsIMutI CAGTG 3 cut(s) 99, 567, 585
Cac8I GCNNGC 3 cut(s) 342, 1772, 1884
CciI TCATGA 1 cut(s) 532
CfoI GCGC 1 cut(s) 423
Cfr13I GGNCC 2 cut(s) 69, 1115
Csp6I GTAC 4 cut(s) 254, 305, 489, 636
CviQI GTAC 4 cut(s) 254, 305, 489, 636
DdeI CTNAG 4 cut(s) 762, 807, 1083, 1214
DpnI GATC 8 cut(s) 59, 265, 295, 687, 1097, 1722, 1755, 1812
DpnII GATC 8 cut(s) 57, 263, 293, 685, 1095, 1720, 1753, 1810
DraI TTTAAA 1 cut(s) 988
DrdI GACNNNNNNGTC 1 cut(s) 664
DseDI GACNNNNNNGTC 1 cut(s) 664
EaeI YGGCCR 2 cut(s) 76, 582
Eam1104I CTCTTC 1 cut(s) 1488
EarI CTCTTC 1 cut(s) 1488
EciI GGCGGA 2 cut(s) 945, 1296
Eco105I TACGTA 1 cut(s) 635
Eco130I CCWWGG 2 cut(s) 141, 599
Eco32I GATATC 2 cut(s) 643, 1836
Eco47I GGWCC 1 cut(s) 1115
Eco57I CTGAAG 2 cut(s) 531, 1932
Eco91I GGTNACC 1 cut(s) 1138
EcoO109I RGGNCCY 1 cut(s) 69
EcoO65I GGTNACC 1 cut(s) 1138
EcoRI GAATTC 1 cut(s) 811
EcoRII CCWGG 1 cut(s) 256
EcoRV GATATC 2 cut(s) 643, 1836
EcoT14I CCWWGG 2 cut(s) 141, 599
EcoT22I ATGCAT 1 cut(s) 1681
ErhI CCWWGG 2 cut(s) 141, 599
Esp3I CGTCTC 1 cut(s) 12
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 4 cut(s) 395, 538, 899, 1537
FauNDI CATATG 1 cut(s) 1888
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 3 cut(s) 1365, 1587, 1783
Fnu4HI GCNGC 2 cut(s) 459, 702
Fsp4HI GCNGC 2 cut(s) 459, 702
FspBI CTAG 7 cut(s) 347, 431, 936, 1313, 1709, 1730, 1917
GlaI GCGC 1 cut(s) 422
GluI GCNGC 2 cut(s) 459, 702
GsuI CTGGAG 2 cut(s) 279, 1366
HaeIII GGCC 6 cut(s) 71, 78, 584, 751, 1670, 1882
HapII CCGG 4 cut(s) 66, 108, 1143, 1671
HhaI GCGC 1 cut(s) 423
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HincII GTYRAC 1 cut(s) 1845
HindII GTYRAC 1 cut(s) 1845
HinfI GANTC 8 cut(s) 34, 104, 321, 369, 803, 1218, 1444, 1934
HpaII CCGG 4 cut(s) 66, 108, 1143, 1671
HphI GGTGA 3 cut(s) 316, 1049, 1150
Hpy166II GTNNAC 6 cut(s) 85, 491, 1366, 1588, 1784, 1845
Hpy188I TCNGA 9 cut(s) 189, 763, 810, 865, 1084, 1100, 1254, 1828, 1939
Hpy188III TCNNGA 7 cut(s) 108, 152, 509, 533, 1005, 1383, 1544
Hpy8I GTNNAC 6 cut(s) 85, 491, 1366, 1588, 1784, 1845
HpyAV CCTTC 3 cut(s) 40, 1027, 1337
HpyCH4III ACNGT 6 cut(s) 571, 612, 622, 745, 1501, 1592
HpyCH4IV ACGT 1 cut(s) 634
HpyF10VI GCNNNNNNNGC 4 cut(s) 337, 564, 1676, 1879
HpyF3I CTNAG 4 cut(s) 762, 807, 1083, 1214
HpySE526I ACGT 1 cut(s) 634
HspAI GCGC 1 cut(s) 421
Kpn2I TCCGGA 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 8 cut(s) 57, 263, 293, 685, 1095, 1720, 1753, 1810
LmnI GCTCC 5 cut(s) 392, 511, 555, 956, 1546
Lsp1109I GCAGC 2 cut(s) 470, 713
MaeI CTAG 7 cut(s) 347, 431, 936, 1313, 1709, 1730, 1917
MaeII ACGT 1 cut(s) 634
MaeIII GTNAC 3 cut(s) 1138, 1532, 1625
MalI GATC 8 cut(s) 59, 265, 295, 687, 1097, 1722, 1755, 1812
MboI GATC 8 cut(s) 57, 263, 293, 685, 1095, 1720, 1753, 1810
MboII GAAGA 5 cut(s) 637, 1020, 1170, 1296, 1505
MflI RGATCY 3 cut(s) 263, 293, 1720
MlsI TGGCCA 1 cut(s) 584
MluNI TGGCCA 1 cut(s) 584
MlyI GAGTC 1 cut(s) 1212
MmeI TCCRAC 3 cut(s) 225, 541, 1258
Mox20I TGGCCA 1 cut(s) 584
Mph1103I ATGCAT 1 cut(s) 1681
MroI TCCGGA 1 cut(s) 107
MroXI GAANNNNTTC 1 cut(s) 516
MscI TGGCCA 1 cut(s) 584
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 584
MspCI CTTAAG 2 cut(s) 770, 1762
MspI CCGG 4 cut(s) 66, 108, 1143, 1671
MspR9I CCNGG 3 cut(s) 67, 258, 1672
Mva1269I GAATGC 1 cut(s) 1269
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 4 cut(s) 337, 564, 1676, 1879
NciI CCSGG 2 cut(s) 67, 1672
NdeI CATATG 1 cut(s) 1888
NdeII GATC 8 cut(s) 57, 263, 293, 685, 1095, 1720, 1753, 1810
NmuCI GTSAC 1 cut(s) 1138
NsiI ATGCAT 1 cut(s) 1681
NspI RCATGY 3 cut(s) 883, 1778, 1963
PagI TCATGA 1 cut(s) 532
PciI ACATGT 1 cut(s) 1959
PctI GAATGC 1 cut(s) 1269
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 7 cut(s) 34, 104, 321, 369, 803, 1444, 1934
PflMI CCANNNNNTGG 2 cut(s) 926, 1889
PkrI GCNGC 2 cut(s) 460, 703
PleI GAGTC 1 cut(s) 1212
PpsI GAGTC 1 cut(s) 1212
Ppu21I YACGTR 1 cut(s) 635
PscI ACATGT 1 cut(s) 1959
PshBI ATTAAT 1 cut(s) 653
PsiI TTATAA 2 cut(s) 1125, 1292
Psp6I CCWGG 1 cut(s) 256
PspEI GGTNACC 1 cut(s) 1138
PspGI CCWGG 1 cut(s) 256
PspPI GGNCC 2 cut(s) 69, 1115
PstI CTGCAG 2 cut(s) 547, 703
PsuI RGATCY 3 cut(s) 263, 293, 1720
RsaI GTAC 4 cut(s) 255, 306, 490, 637
RsaNI GTAC 4 cut(s) 254, 305, 489, 636
RseI CAYNNNNRTG 1 cut(s) 92
SatI GCNGC 2 cut(s) 459, 702
Sau3AI GATC 8 cut(s) 57, 263, 293, 685, 1095, 1720, 1753, 1810
Sau96I GGNCC 2 cut(s) 69, 1115
SchI GAGTC 1 cut(s) 1212
ScrFI CCNGG 3 cut(s) 67, 258, 1672
SfcI CTRYAG 3 cut(s) 543, 699, 1588
SinI GGWCC 1 cut(s) 1115
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 3 cut(s) 770, 1353, 1762
SmoI CTYRAG 3 cut(s) 770, 1353, 1762
SnaBI TACGTA 1 cut(s) 635
SsiI CCGC 3 cut(s) 756, 930, 1281
SspI AATATT 1 cut(s) 353
SspMI CTAG 7 cut(s) 347, 431, 936, 1313, 1709, 1730, 1917
StyD4I CCNGG 3 cut(s) 65, 256, 1670
StyI CCWWGG 2 cut(s) 141, 599
TaaI ACNGT 6 cut(s) 571, 612, 622, 745, 1501, 1592
TaiI ACGT 1 cut(s) 637
TaqI TCGA 2 cut(s) 32, 1004
TfiI GAWTC 7 cut(s) 34, 104, 321, 369, 803, 1444, 1934
TscAI CASTG 3 cut(s) 106, 574, 592
TseFI GTSAC 1 cut(s) 1138
TseI GCWGC 2 cut(s) 458, 701
Tsp45I GTSAC 1 cut(s) 1138
TspGWI ACGGA 1 cut(s) 1038
TspRI CASTG 3 cut(s) 106, 574, 592
Van91I CCANNNNNTGG 2 cut(s) 926, 1889
Vha464I CTTAAG 2 cut(s) 770, 1762
VpaK11BI GGWCC 1 cut(s) 1115
VspI ATTAAT 1 cut(s) 653
XapI RAATTY 8 cut(s) 287, 811, 981, 989, 1476, 1515, 1633, 1941
XceI RCATGY 3 cut(s) 883, 1778, 1963
XmiI GTMKAC 3 cut(s) 1365, 1587, 1783
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 7 cut(s) 347, 431, 936, 1313, 1709, 1730, 1917
Zsp2I ATGCAT 1 cut(s) 1681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.