Rroxscaffold_1G00040350

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000704
Physical Location & Seq
Reverse (-)
58010084 .. 58013365
3282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040350.1

Sequence Viewer

Length: 891 bp
ATGGTTATAGTTGTGCTATACGACGAGTCGTCTAATAGTTTGAGGCACAAAGCAATAAACCCAAAGCCACTTTGTTCAACTGGTTATGGACTTATGGTCTATTTGGGGGAACGGAGTATACCTGAGCTGAATGCTGAGGATCACAAACGTGTCAGAAGTGCGCTTATGCTATTCTTGAAGCCTGAGTCACTGAAGCAATATGTGTGGAAAATGGATGAAGAAATCAGAAAGCACCTTGAGATGCATTGGCATGGCAAACAACAAGTAACACAGCCTCAAGGCAAGCAAGAGGGTTCAAAACATGCTCAAACAACTTATATGCGAAAAGAGGGACATGACACTTCATCTGTTCTGATTACGTTCCTTTTGCGGCTTCTCGCTAATGAACCAGCTGTTTATGCAGCCCTTCTTCAAGAGCAGCAAGAAATTGCTAGAAGCAAATCGGCAGGAGAGTTTCTAACATGGGAAGACCTTGCCAAAATGAAATACACATGGAAAGTAGCACTGGAAACTCTGAGGATGACTCCTCCTGTCTTTGGTGGCTTTAGGACTGCTGTCAAAGATATTGAGTTCGGTGGATTCCACATTCCTAAAGGGCAGATATTCTGGGCTGCTCCTATGACCGACATGGATGATAACATATTCCCGGAGCCATCGAAGTTTGATCCAAGTAGCTTCGACAAGCAAGCAACAGTTCCACCCTACTGCTTTGTACCATTTGGAGGGGGACCTAGAATATGTCCAGGATATGAGTTTGCCAGGATTGCAATACACCATATGGTAACTCAATTCACTTGGAAGCTATGTGCAGACAATCACTTCAGTAGGGATCTAATGCCTGTACCTACTCAAGGACTGGCCATTGAAATCACGCCTAGAATACTACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

296

Amino Acids

33.81

Weight (kDa)

7.73

Isoelectric Point (pI)

55.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 97 - 273 2.8e-39 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 118
AciI CCGC 1 cut(s) 370
AclWI GGATC 3 cut(s) 147, 659, 837
AcoI YGGCCR 1 cut(s) 858
AcuI CTGAAG 2 cut(s) 212, 805
AfaI GTAC 2 cut(s) 714, 843
AfiI CCNNNNNNNGG 3 cut(s) 536, 722, 851
AflIII ACRYGT 1 cut(s) 148
AgsI TTSAA 5 cut(s) 78, 178, 297, 413, 866
AhdI GACNNNNNGTC 2 cut(s) 28, 95
AjnI CCWGG 2 cut(s) 742, 758
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 4 cut(s) 127, 392, 675, 802
AluI AGCT 4 cut(s) 127, 392, 675, 802
AlwI GGATC 3 cut(s) 147, 659, 837
AoxI GGCC 1 cut(s) 858
ApeKI GCWGC 3 cut(s) 401, 418, 611
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 728
AsuC2I CCSGG 1 cut(s) 647
AvaII GGWCC 1 cut(s) 728
BalI TGGCCA 1 cut(s) 860
BbsI GAAGAC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 3 cut(s) 413, 430, 598
BccI CCATC 1 cut(s) 661
BciT130I CCWGG 2 cut(s) 744, 760
BcnI CCSGG 1 cut(s) 647
BfaI CTAG 3 cut(s) 432, 732, 876
BisI GCNGC 4 cut(s) 371, 402, 419, 612
BlsI GCNGC 4 cut(s) 372, 403, 420, 613
Bme1390I CCNGG 3 cut(s) 647, 744, 760
Bme18I GGWCC 1 cut(s) 728
BmeRI GACNNNNNGTC 2 cut(s) 28, 95
BmgT120I GGNCC 1 cut(s) 728
BmiI GGNNCC 2 cut(s) 651, 729
BmrFI CCNGG 3 cut(s) 647, 744, 760
BmsI GCATC 1 cut(s) 231
BoxI GACNNNNGTC 1 cut(s) 554
BpiI GAAGAC 1 cut(s) 474
BplI GAGNNNNNCTC 2 cut(s) 508, 540
Bpu10I CCTNAGC 2 cut(s) 123, 135
BpuEI CTTGAG 3 cut(s) 257, 261, 834
BpuMI CCSGG 1 cut(s) 647
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 722, 851
Bse1I ACTGG 3 cut(s) 85, 510, 861
BseBI CCWGG 2 cut(s) 744, 760
BseGI GGATG 3 cut(s) 220, 525, 637
BseLI CCNNNNNNNGG 3 cut(s) 536, 722, 851
BseMII CTCAG 4 cut(s) 114, 126, 174, 506
BseNI ACTGG 3 cut(s) 85, 510, 861
BseRI GAGGAG 1 cut(s) 516
BseXI GCAGC 3 cut(s) 413, 430, 598
BsgI GTGCAG 1 cut(s) 828
BshFI GGCC 1 cut(s) 860
BsiSI CCGG 1 cut(s) 647
BslFI GGGAC 2 cut(s) 345, 741
BslI CCNNNNNNNGG 3 cut(s) 536, 722, 851
BsmFI GGGAC 2 cut(s) 345, 741
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 1 cut(s) 860
Bsp143I GATC 3 cut(s) 139, 664, 829
BspACI CCGC 1 cut(s) 370
BspANI GGCC 1 cut(s) 860
BspCNI CTCAG 4 cut(s) 115, 127, 175, 507
BspLI GGNNCC 2 cut(s) 651, 729
BspPI GGATC 3 cut(s) 147, 659, 837
BsrI ACTGG 3 cut(s) 85, 510, 861
BssMI GATC 3 cut(s) 139, 664, 829
BssNAI GTATAC 1 cut(s) 119
Bst1107I GTATAC 1 cut(s) 119
Bst2UI CCWGG 2 cut(s) 744, 760
Bst4CI ACNGT 2 cut(s) 694, 888
BstC8I GCNNGC 2 cut(s) 284, 687
BstDEI CTNAG 4 cut(s) 123, 135, 183, 515
BstENI CCTNNNNNAGG 1 cut(s) 849
BstF5I GGATG 3 cut(s) 220, 525, 637
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 3 cut(s) 142, 667, 832
BstMBI GATC 3 cut(s) 139, 664, 829
BstMWI GCNNNNNNNGC 2 cut(s) 398, 764
BstNI CCWGG 2 cut(s) 744, 760
BstNSI RCATGY 1 cut(s) 305
BstPAI GACNNNNGTC 1 cut(s) 554
BstSCI CCNGG 3 cut(s) 645, 742, 758
BstV1I GCAGC 3 cut(s) 413, 430, 598
BstV2I GAAGAC 1 cut(s) 474
BstX2I RGATCY 1 cut(s) 829
BstYI RGATCY 1 cut(s) 829
BstZ17I GTATAC 1 cut(s) 119
BsuRI GGCC 1 cut(s) 860
BtsCI GGATG 3 cut(s) 220, 525, 637
BtsIMutI CAGTG 2 cut(s) 188, 503
Cac8I GCNNGC 2 cut(s) 284, 687
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 728
Csp6I GTAC 2 cut(s) 713, 842
CspCI CAANNNNNGTGG 2 cut(s) 185, 220
CviAII CATG 6 cut(s) 251, 302, 335, 462, 492, 628
CviQI GTAC 2 cut(s) 713, 842
DdeI CTNAG 4 cut(s) 123, 135, 183, 515
DpnI GATC 3 cut(s) 141, 666, 831
DpnII GATC 3 cut(s) 139, 664, 829
DriI GACNNNNNGTC 2 cut(s) 28, 95
EaeI YGGCCR 1 cut(s) 858
Eam1105I GACNNNNNGTC 2 cut(s) 28, 95
Eco47I GGWCC 1 cut(s) 728
Eco57I CTGAAG 2 cut(s) 212, 805
EcoNI CCTNNNNNAGG 1 cut(s) 849
EcoO109I RGGNCCY 1 cut(s) 728
EcoRII CCWGG 2 cut(s) 742, 758
EcoT22I ATGCAT 1 cut(s) 246
FaeI CATG 6 cut(s) 254, 305, 338, 465, 495, 631
FaqI GGGAC 2 cut(s) 345, 741
FatI CATG 6 cut(s) 250, 301, 334, 461, 491, 627
FauNDI CATATG 1 cut(s) 777
FblI GTMKAC 1 cut(s) 118
Fnu4HI GCNGC 4 cut(s) 371, 402, 419, 612
FokI GGATG 3 cut(s) 227, 532, 644
Fsp4HI GCNGC 4 cut(s) 371, 402, 419, 612
FspBI CTAG 3 cut(s) 432, 732, 876
GlaI GCGC 1 cut(s) 162
GluI GCNGC 4 cut(s) 371, 402, 419, 612
HaeIII GGCC 1 cut(s) 860
HapII CCGG 1 cut(s) 647
HhaI GCGC 1 cut(s) 163
Hin1II CATG 6 cut(s) 254, 305, 338, 465, 495, 631
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 4 cut(s) 26, 185, 523, 579
HpaII CCGG 1 cut(s) 647
Hpy166II GTNNAC 1 cut(s) 119
Hpy188I TCNGA 4 cut(s) 155, 227, 354, 516
Hpy188III TCNNGA 2 cut(s) 175, 413
Hpy8I GTNNAC 1 cut(s) 119
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 2 cut(s) 694, 888
HpyCH4IV ACGT 2 cut(s) 148, 359
HpyCH4V TGCA 4 cut(s) 244, 401, 767, 809
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 764
HpyF3I CTNAG 4 cut(s) 123, 135, 183, 515
HpySE526I ACGT 2 cut(s) 148, 359
Hsp92II CATG 6 cut(s) 254, 305, 338, 465, 495, 631
HspAI GCGC 1 cut(s) 161
Kzo9I GATC 3 cut(s) 139, 664, 829
LmnI GCTCC 2 cut(s) 619, 649
Lsp1109I GCAGC 3 cut(s) 413, 430, 598
LweI GCATC 1 cut(s) 231
MaeI CTAG 3 cut(s) 432, 732, 876
MaeII ACGT 2 cut(s) 148, 359
MaeIII GTNAC 3 cut(s) 186, 265, 781
MalI GATC 3 cut(s) 141, 666, 831
MboI GATC 3 cut(s) 139, 664, 829
MboII GAAGA 3 cut(s) 230, 401, 479
MflI RGATCY 1 cut(s) 829
MlsI TGGCCA 1 cut(s) 860
MluCI AATT 2 cut(s) 426, 788
MluNI TGGCCA 1 cut(s) 860
MlyI GAGTC 3 cut(s) 35, 194, 517
MnlI CCTC 8 cut(s) 36, 130, 283, 285, 322, 510, 537, 716
Mox20I TGGCCA 1 cut(s) 860
Mph1103I ATGCAT 1 cut(s) 246
MscI TGGCCA 1 cut(s) 860
MslI CAYNNNNRTG 2 cut(s) 147, 249
Msp20I TGGCCA 1 cut(s) 860
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 1 cut(s) 647
MspR9I CCNGG 3 cut(s) 647, 744, 760
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 2 cut(s) 744, 760
MwoI GCNNNNNNNGC 2 cut(s) 398, 764
NciI CCSGG 1 cut(s) 647
NdeI CATATG 1 cut(s) 777
NdeII GATC 3 cut(s) 139, 664, 829
NlaIII CATG 6 cut(s) 254, 305, 338, 465, 495, 631
NlaIV GGNNCC 2 cut(s) 651, 729
NmuCI GTSAC 1 cut(s) 186
NsiI ATGCAT 1 cut(s) 246
NspI RCATGY 1 cut(s) 305
OliI CACNNNNGTG 1 cut(s) 147
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 1 cut(s) 579
PfoI TCCNGGA 2 cut(s) 645, 742
PkrI GCNGC 4 cut(s) 372, 403, 420, 613
PleI GAGTC 3 cut(s) 34, 193, 517
PpsI GAGTC 3 cut(s) 34, 193, 517
PpuMI RGGWCCY 1 cut(s) 728
PshAI GACNNNNGTC 1 cut(s) 554
Psp5II RGGWCCY 1 cut(s) 728
Psp6I CCWGG 2 cut(s) 742, 758
PspGI CCWGG 2 cut(s) 742, 758
PspN4I GGNNCC 2 cut(s) 651, 729
PspPI GGNCC 1 cut(s) 728
PspPPI RGGWCCY 1 cut(s) 728
PsrI GAACNNNNNNTAC 2 cut(s) 102, 134
PsuI RGATCY 1 cut(s) 829
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 2 cut(s) 714, 843
RsaNI GTAC 2 cut(s) 713, 842
RseI CAYNNNNRTG 2 cut(s) 147, 249
SatI GCNGC 4 cut(s) 371, 402, 419, 612
Sau3AI GATC 3 cut(s) 139, 664, 829
Sau96I GGNCC 1 cut(s) 728
SchI GAGTC 3 cut(s) 35, 194, 517
ScrFI CCNGG 3 cut(s) 647, 744, 760
SfaNI GCATC 1 cut(s) 231
SinI GGWCC 1 cut(s) 728
SmiMI CAYNNNNRTG 2 cut(s) 147, 249
SmlI CTYRAG 3 cut(s) 236, 276, 849
SmoI CTYRAG 3 cut(s) 236, 276, 849
Sse9I AATT 2 cut(s) 426, 788
SsiI CCGC 1 cut(s) 370
SspMI CTAG 3 cut(s) 432, 732, 876
StyD4I CCNGG 3 cut(s) 645, 742, 758
TaaI ACNGT 2 cut(s) 694, 888
TaiI ACGT 2 cut(s) 151, 362
TaqI TCGA 2 cut(s) 656, 678
TaqII GACCGA 1 cut(s) 638
TasI AATT 2 cut(s) 426, 788
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 1 cut(s) 579
TscAI CASTG 2 cut(s) 195, 510
TseFI GTSAC 1 cut(s) 186
TseI GCWGC 3 cut(s) 401, 418, 611
Tsp45I GTSAC 1 cut(s) 186
TspDTI ATGAA 4 cut(s) 231, 333, 399, 497
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 2 cut(s) 195, 510
VpaK11BI GGWCC 1 cut(s) 728
XagI CCTNNNNNAGG 1 cut(s) 849
XceI RCATGY 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 118
XspI CTAG 3 cut(s) 432, 732, 876
Zsp2I ATGCAT 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.