FvH4_3g22440

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000704
Physical Location & Seq
Reverse (-)
15487317 .. 15489482
2166 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22440.t1

Sequence Viewer

Length: 1422 bp
ATGAACACCCTTTTTGCAGTTTTCCTCTTCCTGCTTGTTCCCTTATCCCTTCTCCTTAGGAGAAGAAAAAAGTCATCAAAAAGACTTCCACCGGGCTCATTAGGACTACCCCTCATAGGCCAGAGCCTTGATTTCCTCAGAGCCATGCGTGCCAACACTGCAGAGAAATGGCTTGAACAAAGGATCAGAAAATATGGTCCTGTTTCGAAGCTGAGTCTTTTTGGCAAGCCAACAGTCTTCATCCATGGACAAGCTGCGAACAAGTTTGTATTCACCACTGATGGGAGCACCATTTCCAGCCAACAGGCTGAATCGACTAAAAGAATTCTGGGGGAGCGGAGTATATTTGAGCTGAGTATCGAGGATCACAAGCGTGTAAGAAGTGCTCTTATGCTATTCTTGAAGCCTGAGTCACTGAAGCAATATGTGGGGAAAATGGACGAAGAAATCAGGAAGCACCTTGAAATGCATTGGCATGGCAAACAACAAGTAACGGTCTTGCCCCTGATGAAGAACCTCACGTTCAACATAATATGCTCTCTACTTTTTGGGCTTGAAAGAGGAATTCGCAGAGAACAATTTGTGGAGTGCTTTCAAACAATGATTGAAGGAATGTGGTCAGTGCCGGTCAACTTTCCCTTCACGCGTTACAACAGCAGCCTCAAGGCAAGCAAGAGGGTTCAAAACATGATCAAAGAACTTATACGCGAAAAGAGGTTACAGCTTGAGCAAAAAACGGCTTCTCCACAACATGATCTCATTACTTGCTTCCTTAGCATCCGAAATGATGAAGGTAAAGAAGAGCTGACAGAGTTGGAGATGGTGCACAATGTCATTGTAGTCATGGTTGCTGGACATGACACTTCATCTGTTCTGATTACGTTCCTTTTGCAGCTTCTAGCTAATGAACCAGTTGTTTATGCAGCCCTTCTACAAGAGCAGGAAGAAATAGCTAGAAGAAAATCGGCAGGAGAGTTTCTAACTTGGGAAGACCTTGCCAAAATGAAATACACATGGAGAGTAGCACAAGAAACTCTGAGGATCACTCCTCCTGTGTTTGGTACCTTCAGGACTGCTCTCAAAGATATTGAGTTTGGCGGATTCCACATTCCTAAAGGGTGGCAGATATTATGGGTTGCTCCTATGACCCACATGGACGAGAGCATATTCCCAGAACCCGCAAAGTTTGATCCAAGTAGGTTCGACAAGCAAGCATCAGTTCCACCCTACTGCTTCGTACCATTCGGAGGGGGACCTAAAATATGTCCAGGATATGAGTTTGCCAGGATCGAAACCCTTATTGCAATGCACCATATCGTAACTCAGTTCACCTGGAAGCTATGTGCAGACAATCACTTCAGTAGGGCTCCAATGCCGGTACCTACCGGAGGACTGCCCATTGAAATCAAGCCTAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

474

Amino Acids

54.25

Weight (kDa)

9.45

Isoelectric Point (pI)

61.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 30 - 452 7.1e-77 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1061, 1378
AccB1I GGYRCC 2 cut(s) 1061, 1378
AccBSI CCGCTC 1 cut(s) 337
AccII CGCG 2 cut(s) 646, 708
AciI CCGC 3 cut(s) 337, 1098, 1179
AclWI GGATC 5 cut(s) 191, 372, 1049, 1184, 1295
AcsI RAATTY 2 cut(s) 324, 564
AcuI CTGAAG 3 cut(s) 437, 1051, 1342
AfaI GTAC 3 cut(s) 1063, 1239, 1380
AfiI CCNNNNNNNGG 5 cut(s) 116, 282, 1058, 1247, 1388
AflIII ACRYGT 1 cut(s) 644
AgsI TTSAA 9 cut(s) 176, 403, 464, 526, 557, 596, 608, 683, 1403
AjnI CCWGG 3 cut(s) 1267, 1283, 1331
AleI CACNNNNGTG 1 cut(s) 372
AluBI AGCT 9 cut(s) 211, 254, 352, 724, 805, 895, 902, 953, 1339
AluI AGCT 9 cut(s) 211, 254, 352, 724, 805, 895, 902, 953, 1339
Alw21I GWGCWC 3 cut(s) 290, 388, 828
Alw44I GTGCAC 1 cut(s) 824
AlwI GGATC 5 cut(s) 191, 372, 1049, 1184, 1295
AoxI GGCC 1 cut(s) 118
ApaLI GTGCAC 1 cut(s) 824
ApeKI GCWGC 4 cut(s) 254, 657, 892, 923
ApoI RAATTY 2 cut(s) 324, 564
Asp718I GGTACC 2 cut(s) 1061, 1378
AspS9I GGNCC 2 cut(s) 197, 1253
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 2 cut(s) 265, 1321
AsuII TTCGAA 1 cut(s) 206
AvaII GGWCC 2 cut(s) 197, 1253
AxyI CCTNAGG 1 cut(s) 56
BaeGI GKGCMC 1 cut(s) 828
BanI GGYRCC 2 cut(s) 1061, 1378
BanII GRGCYC 2 cut(s) 98, 1369
BbsI GAAGAC 2 cut(s) 229, 996
Bbv12I GWGCWC 3 cut(s) 290, 388, 828
BbvI GCAGC 4 cut(s) 241, 669, 904, 935
BccI CCATC 2 cut(s) 275, 814
BceAI ACGGC 1 cut(s) 753
BciT130I CCWGG 3 cut(s) 1269, 1285, 1333
BclI TGATCA 1 cut(s) 690
BcnI CCSGG 1 cut(s) 93
BfaI CTAG 3 cut(s) 899, 954, 1413
BfmI CTRYAG 1 cut(s) 159
BisI GCNGC 4 cut(s) 255, 658, 893, 924
BlsI GCNGC 4 cut(s) 256, 659, 894, 925
Bme1390I CCNGG 4 cut(s) 93, 1269, 1285, 1333
Bme18I GGWCC 2 cut(s) 197, 1253
BmgT120I GGNCC 2 cut(s) 197, 1253
BmiI GGNNCC 4 cut(s) 1063, 1254, 1368, 1380
BmrFI CCNGG 4 cut(s) 93, 1269, 1285, 1333
BmsI GCATC 2 cut(s) 786, 1223
BpiI GAAGAC 2 cut(s) 229, 996
BplI GAGNNNNNCTC 2 cut(s) 1030, 1062
Bpu10I CCTNAGC 1 cut(s) 773
Bpu14I TTCGAA 1 cut(s) 206
BpuEI CTTGAG 2 cut(s) 647, 746
BpuMI CCSGG 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 244
BsaWI WCCGGW 1 cut(s) 1385
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
Bsc4I CCNNNNNNNGG 5 cut(s) 116, 282, 1058, 1247, 1388
Bse118I RCCGGY 2 cut(s) 625, 1375
Bse1I ACTGG 1 cut(s) 911
Bse21I CCTNAGG 1 cut(s) 56
Bse3DI GCAATG 1 cut(s) 1311
BseBI CCWGG 3 cut(s) 1269, 1285, 1333
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 2 cut(s) 240, 777
BseLI CCNNNNNNNGG 5 cut(s) 116, 282, 1058, 1247, 1388
BseMI GCAATG 1 cut(s) 1311
BseMII CTCAG 6 cut(s) 151, 203, 344, 399, 1028, 1337
BseNI ACTGG 1 cut(s) 911
BseRI GAGGAG 1 cut(s) 1038
BseSI GKGCMC 1 cut(s) 828
BseXI GCAGC 4 cut(s) 241, 669, 904, 935
BsgI GTGCAG 1 cut(s) 1365
Bsh1236I CGCG 2 cut(s) 646, 708
BshFI GGCC 1 cut(s) 120
BshNI GGYRCC 2 cut(s) 1061, 1378
BsiHKAI GWGCWC 3 cut(s) 290, 388, 828
BsiSI CCGG 4 cut(s) 92, 626, 1376, 1386
BslFI GGGAC 1 cut(s) 1266
BslI CCNNNNNNNGG 5 cut(s) 116, 282, 1058, 1247, 1388
BsmFI GGGAC 1 cut(s) 1266
BsnI GGCC 1 cut(s) 120
Bsp119I TTCGAA 1 cut(s) 206
Bsp1286I GDGCHC 5 cut(s) 98, 290, 388, 828, 1369
Bsp143I GATC 7 cut(s) 183, 364, 690, 754, 1041, 1189, 1287
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 3 cut(s) 337, 1098, 1179
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 6 cut(s) 150, 204, 345, 400, 1029, 1336
BspFNI CGCG 2 cut(s) 646, 708
BspLI GGNNCC 4 cut(s) 1063, 1254, 1368, 1380
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 5 cut(s) 191, 372, 1049, 1184, 1295
BspQI GCTCTTC 1 cut(s) 795
BspT104I TTCGAA 1 cut(s) 206
BspT107I GGYRCC 2 cut(s) 1061, 1378
BsrBI CCGCTC 1 cut(s) 337
BsrDI GCAATG 1 cut(s) 1311
BsrFI RCCGGY 2 cut(s) 625, 1375
BsrI ACTGG 1 cut(s) 911
BssAI RCCGGY 2 cut(s) 625, 1375
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 7 cut(s) 183, 364, 690, 754, 1041, 1189, 1287
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 3 cut(s) 1269, 1285, 1333
Bst4CI ACNGT 2 cut(s) 235, 496
Bst6I CTCTTC 2 cut(s) 32, 795
BstBI TTCGAA 1 cut(s) 206
BstC8I GCNNGC 4 cut(s) 150, 227, 670, 1212
BstDEI CTNAG 8 cut(s) 56, 137, 212, 353, 408, 773, 1037, 1323
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 1386
BstF5I GGATG 2 cut(s) 240, 777
BstFNI CGCG 2 cut(s) 646, 708
BstKTI GATC 7 cut(s) 186, 367, 693, 757, 1044, 1192, 1290
BstMBI GATC 7 cut(s) 183, 364, 690, 754, 1041, 1189, 1287
BstMWI GCNNNNNNNGC 3 cut(s) 149, 158, 774
BstNI CCWGG 3 cut(s) 1269, 1285, 1333
BstSCI CCNGG 4 cut(s) 91, 1267, 1283, 1331
BstSFI CTRYAG 1 cut(s) 159
BstSLI GKGCMC 1 cut(s) 828
BstUI CGCG 2 cut(s) 646, 708
BstV1I GCAGC 4 cut(s) 241, 669, 904, 935
BstV2I GAAGAC 2 cut(s) 229, 996
Bsu36I CCTNAGG 1 cut(s) 56
BsuRI GGCC 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 2 cut(s) 240, 777
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 4 cut(s) 156, 276, 413, 627
Cac8I GCNNGC 4 cut(s) 150, 227, 670, 1212
Cfr10I RCCGGY 2 cut(s) 625, 1375
Cfr13I GGNCC 2 cut(s) 197, 1253
Csp6I GTAC 3 cut(s) 1062, 1238, 1379
CviQI GTAC 3 cut(s) 1062, 1238, 1379
DdeI CTNAG 8 cut(s) 56, 137, 212, 353, 408, 773, 1037, 1323
DpnI GATC 7 cut(s) 185, 366, 692, 756, 1043, 1191, 1289
DpnII GATC 7 cut(s) 183, 364, 690, 754, 1041, 1189, 1287
Eam1104I CTCTTC 2 cut(s) 32, 795
EarI CTCTTC 2 cut(s) 32, 795
EciI GGCGGA 1 cut(s) 1113
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 2 cut(s) 98, 1369
Eco47I GGWCC 2 cut(s) 197, 1253
Eco57I CTGAAG 3 cut(s) 437, 1051, 1342
Eco81I CCTNAGG 1 cut(s) 56
EcoNI CCTNNNNNAGG 1 cut(s) 1386
EcoO109I RGGNCCY 1 cut(s) 1253
EcoRI GAATTC 2 cut(s) 324, 564
EcoRII CCWGG 3 cut(s) 1267, 1283, 1331
EcoT14I CCWWGG 1 cut(s) 244
EcoT22I ATGCAT 1 cut(s) 471
EcoT38I GRGCYC 2 cut(s) 98, 1369
ErhI CCWWGG 1 cut(s) 244
FaqI GGGAC 1 cut(s) 1266
FauI CCCGC 1 cut(s) 1186
FbaI TGATCA 1 cut(s) 690
Fnu4HI GCNGC 4 cut(s) 255, 658, 893, 924
FokI GGATG 2 cut(s) 227, 764
FriOI GRGCYC 2 cut(s) 98, 1369
Fsp4HI GCNGC 4 cut(s) 255, 658, 893, 924
FspBI CTAG 3 cut(s) 899, 954, 1413
GluI GCNGC 4 cut(s) 255, 658, 893, 924
HaeIII GGCC 1 cut(s) 120
HapII CCGG 4 cut(s) 92, 626, 1376, 1386
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 4 cut(s) 214, 311, 410, 1101
HpaII CCGG 4 cut(s) 92, 626, 1376, 1386
HphI GGTGA 2 cut(s) 265, 1321
Hpy166II GTNNAC 3 cut(s) 631, 826, 1329
Hpy188I TCNGA 6 cut(s) 140, 188, 782, 876, 1038, 1247
Hpy188III TCNNGA 3 cut(s) 400, 451, 1069
Hpy8I GTNNAC 3 cut(s) 631, 826, 1329
HpyAV CCTTC 6 cut(s) 59, 602, 649, 785, 938, 1075
HpyCH4III ACNGT 2 cut(s) 235, 496
HpyCH4IV ACGT 2 cut(s) 521, 881
HpyCH4V TGCA 9 cut(s) 17, 161, 469, 826, 892, 923, 1304, 1309, 1346
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 158, 774
HpyF3I CTNAG 8 cut(s) 56, 137, 212, 353, 408, 773, 1037, 1323
HpySE526I ACGT 2 cut(s) 521, 881
KpnI GGTACC 2 cut(s) 1065, 1382
Ksp22I TGATCA 1 cut(s) 690
Kzo9I GATC 7 cut(s) 183, 364, 690, 754, 1041, 1189, 1287
LguI GCTCTTC 1 cut(s) 795
LmnI GCTCC 4 cut(s) 285, 334, 1144, 1372
Lsp1109I GCAGC 4 cut(s) 241, 669, 904, 935
LweI GCATC 2 cut(s) 786, 1223
MaeI CTAG 3 cut(s) 899, 954, 1413
MaeII ACGT 2 cut(s) 521, 881
MaeIII GTNAC 5 cut(s) 411, 490, 647, 717, 1318
MalI GATC 7 cut(s) 185, 366, 692, 756, 1043, 1191, 1289
MbiI CCGCTC 1 cut(s) 337
MboI GATC 7 cut(s) 183, 364, 690, 754, 1041, 1189, 1287
MboII GAAGA 9 cut(s) 19, 75, 229, 455, 523, 812, 956, 969, 1001
MhlI GDGCHC 5 cut(s) 98, 290, 388, 828, 1369
MluCI AATT 3 cut(s) 324, 564, 578
MluI ACGCGT 1 cut(s) 644
MlyI GAGTC 2 cut(s) 223, 419
MmeI TCCRAC 1 cut(s) 795
Mph1103I ATGCAT 1 cut(s) 471
MslI CAYNNNNRTG 2 cut(s) 372, 474
MspI CCGG 4 cut(s) 92, 626, 1376, 1386
MspR9I CCNGG 4 cut(s) 93, 1269, 1285, 1333
MvaI CCWGG 3 cut(s) 1269, 1285, 1333
MvnI CGCG 2 cut(s) 646, 708
MwoI GCNNNNNNNGC 3 cut(s) 149, 158, 774
NciI CCSGG 1 cut(s) 93
NcoI CCATGG 1 cut(s) 244
NdeII GATC 7 cut(s) 183, 364, 690, 754, 1041, 1189, 1287
NlaIV GGNNCC 4 cut(s) 1063, 1254, 1368, 1380
NmuCI GTSAC 1 cut(s) 411
NsiI ATGCAT 1 cut(s) 471
NspV TTCGAA 1 cut(s) 206
OliI CACNNNNGTG 1 cut(s) 372
PciSI GCTCTTC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 311, 1101
PfoI TCCNGGA 1 cut(s) 1267
PkrI GCNGC 4 cut(s) 256, 659, 894, 925
PleI GAGTC 2 cut(s) 222, 418
PpsI GAGTC 2 cut(s) 222, 418
PpuMI RGGWCCY 1 cut(s) 1253
Psp5II RGGWCCY 1 cut(s) 1253
Psp6I CCWGG 3 cut(s) 1267, 1283, 1331
PspGI CCWGG 3 cut(s) 1267, 1283, 1331
PspN4I GGNNCC 4 cut(s) 1063, 1254, 1368, 1380
PspPI GGNCC 2 cut(s) 197, 1253
PspPPI RGGWCCY 1 cut(s) 1253
PstI CTGCAG 1 cut(s) 163
RsaI GTAC 3 cut(s) 1063, 1239, 1380
RsaNI GTAC 3 cut(s) 1062, 1238, 1379
RseI CAYNNNNRTG 2 cut(s) 372, 474
SapI GCTCTTC 1 cut(s) 795
SatI GCNGC 4 cut(s) 255, 658, 893, 924
Sau3AI GATC 7 cut(s) 183, 364, 690, 754, 1041, 1189, 1287
Sau96I GGNCC 2 cut(s) 197, 1253
SchI GAGTC 2 cut(s) 223, 419
ScrFI CCNGG 4 cut(s) 93, 1269, 1285, 1333
SduI GDGCHC 5 cut(s) 98, 290, 388, 828, 1369
SfaNI GCATC 2 cut(s) 786, 1223
SfcI CTRYAG 1 cut(s) 159
SfuI TTCGAA 1 cut(s) 206
SinI GGWCC 2 cut(s) 197, 1253
SmiMI CAYNNNNRTG 2 cut(s) 372, 474
SmlI CTYRAG 2 cut(s) 662, 725
SmoI CTYRAG 2 cut(s) 662, 725
Sse9I AATT 3 cut(s) 324, 564, 578
SsiI CCGC 3 cut(s) 337, 1098, 1179
SspMI CTAG 3 cut(s) 899, 954, 1413
StyD4I CCNGG 4 cut(s) 91, 1267, 1283, 1331
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 2 cut(s) 235, 496
TaiI ACGT 2 cut(s) 524, 884
TaqI TCGA 5 cut(s) 206, 314, 360, 1203, 1290
TasI AATT 3 cut(s) 324, 564, 578
TfiI GAWTC 2 cut(s) 311, 1101
TscAI CASTG 4 cut(s) 163, 283, 420, 627
TseFI GTSAC 1 cut(s) 411
TseI GCWGC 4 cut(s) 254, 657, 892, 923
Tsp45I GTSAC 1 cut(s) 411
TspDTI ATGAA 7 cut(s) 17, 229, 524, 804, 855, 921, 1019
TspRI CASTG 4 cut(s) 163, 283, 420, 627
VneI GTGCAC 1 cut(s) 824
VpaK11BI GGWCC 2 cut(s) 197, 1253
XagI CCTNNNNNAGG 1 cut(s) 1386
XapI RAATTY 2 cut(s) 324, 564
XspI CTAG 3 cut(s) 899, 954, 1413
Zsp2I ATGCAT 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.