pycom07g21130

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000704
Physical Location & Seq
Forward (+)
22974068 .. 22974706
639 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21130.2

Sequence Viewer

Length: 522 bp
ATGTGCACGTTATATACCATGACAGAGCAAGAGGAAGTAGTGAAGGCTGTGGAAGCAAGGGATGATGGAAAGCTTAGATGGAGTGAAATCCAAATGATGAAGCACACATGGAGAGTGGCCCAAGAGCTCATGAGATTGAACCCTCCAGTTTTCGGCAACTTCAAATGTGCTTCCAGAGACACATCCTTTGATGGCTTTGATATCCCCAAGGGATGGAAGGTTTTTTGGGTGGCATCTGCAACACACATGGACGACAAGATATTTGAAGATCCAAACAAGTTTGATCCATCACGATTCGCGAGTTCATCAAAATCATCATATCCACCGTACACGTACATTCCATTTGGCGCAGGTCCACGGATATGCCCCGGGCTAGAATTTGCAAGAATTGAAGTGTTGCTCATCATTCATCATCTAACTAAGAATTATTCTTGGACACCAATGATCAAGGACGAACCCATAAGACGTGAACCACTGCCCTACCCAGCCATGGGACTTCCAGTTAAGCTTCATAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

174

Amino Acids

20.07

Weight (kDa)

8.72

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 341
AccB7I CCANNNNNTGG 1 cut(s) 213
AccII CGCG 1 cut(s) 299
AclWI GGATC 2 cut(s) 263, 278
AcsI RAATTY 1 cut(s) 377
AfaI GTAC 2 cut(s) 329, 335
AfiI CCNNNNNNNGG 4 cut(s) 152, 213, 490, 491
AflIII ACRYGT 1 cut(s) 330
AgsI TTSAA 4 cut(s) 139, 163, 266, 392
AjiI CACGTC 1 cut(s) 467
AluBI AGCT 3 cut(s) 73, 127, 508
AluI AGCT 3 cut(s) 73, 127, 508
Alw21I GWGCWC 2 cut(s) 8, 129
Alw26I GTCTC 1 cut(s) 171
Alw44I GTGCAC 1 cut(s) 4
AlwI GGATC 2 cut(s) 263, 278
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 1 cut(s) 117
ApaLI GTGCAC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 377
ArsI GACNNNNNNTTYG 4 cut(s) 170, 202, 245, 277
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 2 cut(s) 118, 353
AsuC2I CCSGG 2 cut(s) 369, 370
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 129
Bbv12I GWGCWC 2 cut(s) 8, 129
BccI CCATC 5 cut(s) 59, 72, 185, 207, 295
BclI TGATCA 1 cut(s) 444
BcnI CCSGG 2 cut(s) 369, 370
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 374
BfuAI ACCTGC 1 cut(s) 341
Bme1390I CCNGG 2 cut(s) 369, 370
Bme18I GGWCC 1 cut(s) 353
BmeT110I CYCGRG 1 cut(s) 368
BmgBI CACGTC 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 118, 353
BmrFI CCNGG 2 cut(s) 369, 370
BmsI GCATC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 129
BpuMI CCSGG 2 cut(s) 369, 370
BsaAI YACGTR 1 cut(s) 333
BsaJI CCNNGG 5 cut(s) 207, 356, 367, 368, 489
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 213, 490, 491
Bse1I ACTGG 2 cut(s) 146, 500
BseDI CCNNGG 5 cut(s) 207, 356, 367, 368, 489
BseGI GGATG 3 cut(s) 67, 182, 218
BseLI CCNNNNNNNGG 4 cut(s) 152, 213, 490, 491
BseNI ACTGG 2 cut(s) 146, 500
BseSI GKGCMC 1 cut(s) 8
BseYI CCCAGC 1 cut(s) 484
Bsh1236I CGCG 1 cut(s) 299
BshFI GGCC 1 cut(s) 119
BsiHKAI GWGCWC 2 cut(s) 8, 129
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 507
BslI CCNNNNNNNGG 4 cut(s) 152, 213, 490, 491
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 507
BsnI GGCC 1 cut(s) 119
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 8, 129
Bsp143I GATC 3 cut(s) 268, 283, 444
Bsp19I CCATGG 1 cut(s) 489
Bsp68I TCGCGA 1 cut(s) 299
BspANI GGCC 1 cut(s) 119
BspFNI CGCG 1 cut(s) 299
BspHI TCATGA 1 cut(s) 129
BspMI ACCTGC 1 cut(s) 341
BspPI GGATC 2 cut(s) 263, 278
BsrI ACTGG 2 cut(s) 146, 500
BssECI CCNNGG 5 cut(s) 207, 356, 367, 368, 489
BssMI GATC 3 cut(s) 268, 283, 444
BssT1I CCWWGG 2 cut(s) 207, 489
Bst4CI ACNGT 1 cut(s) 327
BstBAI YACGTR 1 cut(s) 333
BstDEI CTNAG 2 cut(s) 74, 420
BstDSI CCRYGG 2 cut(s) 356, 489
BstF5I GGATG 3 cut(s) 67, 182, 218
BstFNI CGCG 1 cut(s) 299
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 3 cut(s) 271, 286, 447
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 3 cut(s) 268, 283, 444
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstSCI CCNGG 2 cut(s) 367, 368
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BsuRI GGCC 1 cut(s) 119
BtgI CCRYGG 2 cut(s) 356, 489
BtrI CACGTC 1 cut(s) 467
BtsCI GGATG 3 cut(s) 67, 182, 218
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 1 cut(s) 473
BtuMI TCGCGA 1 cut(s) 299
BveI ACCTGC 1 cut(s) 341
CciI TCATGA 1 cut(s) 129
CfoI GCGC 1 cut(s) 350
Cfr13I GGNCC 2 cut(s) 118, 353
Cfr9I CCCGGG 1 cut(s) 368
Csp6I GTAC 2 cut(s) 328, 334
CviAII CATG 5 cut(s) 19, 108, 130, 247, 490
CviJI RGCY 8 cut(s) 47, 73, 119, 127, 195, 373, 488, 508
CviKI_1 RGCY 8 cut(s) 47, 73, 119, 127, 195, 373, 488, 508
CviQI GTAC 2 cut(s) 328, 334
DdeI CTNAG 2 cut(s) 74, 420
DpnI GATC 3 cut(s) 270, 285, 446
DpnII GATC 3 cut(s) 268, 283, 444
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 2 cut(s) 207, 489
Eco24I GRGCYC 1 cut(s) 129
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 127
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 2 cut(s) 207, 489
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 2 cut(s) 207, 489
FaeI CATG 5 cut(s) 22, 111, 133, 250, 493
FaqI GGGAC 1 cut(s) 507
FatI CATG 5 cut(s) 18, 107, 129, 246, 489
FbaI TGATCA 1 cut(s) 444
FokI GGATG 3 cut(s) 74, 169, 225
FriOI GRGCYC 1 cut(s) 129
FspBI CTAG 1 cut(s) 374
GlaI GCGC 1 cut(s) 349
GsaI CCCAGC 1 cut(s) 488
GsuI CTGGAG 1 cut(s) 129
HaeIII GGCC 1 cut(s) 119
HapII CCGG 1 cut(s) 369
HhaI GCGC 1 cut(s) 350
Hin1II CATG 5 cut(s) 22, 111, 133, 250, 493
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HindIII AAGCTT 2 cut(s) 71, 506
HinfI GANTC 1 cut(s) 294
HpaII CCGG 1 cut(s) 369
Hpy166II GTNNAC 4 cut(s) 6, 330, 356, 470
Hpy188III TCNNGA 4 cut(s) 130, 174, 291, 298
Hpy8I GTNNAC 4 cut(s) 6, 330, 356, 470
HpyAV CCTTC 2 cut(s) 37, 211
HpyCH4III ACNGT 1 cut(s) 327
HpyCH4IV ACGT 3 cut(s) 8, 332, 466
HpyCH4V TGCA 3 cut(s) 6, 239, 383
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpyF3I CTNAG 2 cut(s) 74, 420
HpySE526I ACGT 3 cut(s) 8, 332, 466
Hsp92II CATG 5 cut(s) 22, 111, 133, 250, 493
HspAI GCGC 1 cut(s) 348
Ksp22I TGATCA 1 cut(s) 444
Kzo9I GATC 3 cut(s) 268, 283, 444
LpnPI CCDG 6 cut(s) 159, 187, 336, 382, 498, 513
LweI GCATC 1 cut(s) 242
MaeI CTAG 1 cut(s) 374
MaeII ACGT 3 cut(s) 8, 332, 466
MalI GATC 3 cut(s) 270, 285, 446
MboI GATC 3 cut(s) 268, 283, 444
MboII GAAGA 1 cut(s) 278
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 2 cut(s) 8, 129
MluCI AATT 3 cut(s) 377, 387, 424
MnlI CCTC 2 cut(s) 25, 153
MseI TTAA 1 cut(s) 504
MslI CAYNNNNRTG 1 cut(s) 361
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 2 cut(s) 369, 370
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 1 cut(s) 53
NciI CCSGG 2 cut(s) 369, 370
NcoI CCATGG 1 cut(s) 489
NdeII GATC 3 cut(s) 268, 283, 444
NlaIII CATG 5 cut(s) 22, 111, 133, 250, 493
NruI TCGCGA 1 cut(s) 299
PagI TCATGA 1 cut(s) 129
PfeI GAWTC 1 cut(s) 294
PflMI CCANNNNNTGG 1 cut(s) 213
Ppu21I YACGTR 1 cut(s) 333
Psp124BI GAGCTC 1 cut(s) 129
PspFI CCCAGC 1 cut(s) 484
PspPI GGNCC 2 cut(s) 118, 353
PsuI RGATCY 1 cut(s) 268
RruI TCGCGA 1 cut(s) 299
RsaI GTAC 2 cut(s) 329, 335
RsaNI GTAC 2 cut(s) 328, 334
RseI CAYNNNNRTG 1 cut(s) 361
SacI GAGCTC 1 cut(s) 129
SaqAI TTAA 1 cut(s) 504
Sau3AI GATC 3 cut(s) 268, 283, 444
Sau96I GGNCC 2 cut(s) 118, 353
ScrFI CCNGG 2 cut(s) 369, 370
SduI GDGCHC 2 cut(s) 8, 129
SetI ASST 8 cut(s) 11, 75, 129, 222, 335, 355, 469, 510
SfaNI GCATC 1 cut(s) 242
SinI GGWCC 1 cut(s) 353
SmaI CCCGGG 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 361
Sse9I AATT 3 cut(s) 377, 387, 424
SspMI CTAG 1 cut(s) 374
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 2 cut(s) 367, 368
StyI CCWWGG 2 cut(s) 207, 489
TaaI ACNGT 1 cut(s) 327
TaiI ACGT 3 cut(s) 11, 335, 469
TasI AATT 3 cut(s) 377, 387, 424
TfiI GAWTC 1 cut(s) 294
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TscAI CASTG 1 cut(s) 480
TspDTI ATGAA 4 cut(s) 113, 294, 398, 500
TspGWI ACGGA 1 cut(s) 373
TspMI CCCGGG 1 cut(s) 368
TspRI CASTG 1 cut(s) 480
Van91I CCANNNNNTGG 1 cut(s) 213
VneI GTGCAC 1 cut(s) 4
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 1 cut(s) 377
XmaI CCCGGG 1 cut(s) 368
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.