RchiOBHm_Chr5g0039121

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000704
Physical Location & Seq
Reverse (-)
33878532 .. 33882575
4044 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31770

Sequence Viewer

Length: 1071 bp
ATGATTTCAAGGGTTGTGATCATATATACTTTCTTTCCTAGAATCTCCCATTTATACTCAACTCTCACTCCTTTCAATCTACAAAGGTTTGCAAACTCTCTCTCTCTCTCACAAACAAAGCCTCACAAAACCATGATGACTACTCTTTTCACAATTATCCTCTTCCTCATTCCCATATACCTTCTCCTAAAGAGCAGAGAAAAATCATCAGAAAGGCTTCCTCCAGGCTCATTAGGACTGCCCCTAATAGGCCAGAGCCTTGATTTTCTCCGAGCGATGCGTGCCAACACTGCAGAGGATTGGCTTGAACAAAGGATCAGAAAGTATGGTCCAGTTTCAAAGCTAAGTCTCTTTGGCAAGCCAACAGTCTTCATCCATGGACAAGCTGCAAACAAGTTTATATTCACCAGTGATGGGAGCACCATTTCAGCCCAACAAACCGAGTCTATTCGTAGGATTTTGGGGGAACGGAGTATATCTGAGTTAAGTGCTGAGGATCACAAACGTGTCAGAAGTGCACTTATGCTATTCTTGAGGCCTGAGTCACTGAAGCAATATGTGTGGAAAATGGATGAAGAAATCAGGAAGCACCTTGAGATGCACTGGCATGGCAAACAACAAGTAACAATGATGCAAGGATTGTGGTCAGTGCCGGTCAACTTGCCCTTCAAGCGTTACAACAGCAGCCTCAAGGCAAGCAAGAGGGTTCAAAAAATGCTCAAAGAACTTATACGCGAAAGGAGGGTACAACTTGATCAAAAAAGGGCTTCTCCACAACAAGACCTCATCACTTGCTTCCTTAGCATCCGGAATGATGAAGGTCAAGAAGAATTAACAGAGTTGGAGATGGTGCACAATGTCATGCAGGAAGAAATAGCTAGAAGCAAATTGGCGGGAGAATTTTTAACATGGGAAGATCTTGCCAAAATGAAATACACATGGAGTGTAGCACTGGAAACTCTGAGAATGATTCCTCCTTCCTTTGGTAGCTTTAGGACAGCTGTCAAAGATATTGAGTTCGGTGGATTCCACATTCCTAAAGGGTGGCAGATATTCTGGGCACTCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

356

Amino Acids

41.41

Weight (kDa)

9.86

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 288 - 353 7.1e-11 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 735
AccIII TCCGGA 1 cut(s) 807
AciI CCGC 1 cut(s) 893
AclWI GGATC 2 cut(s) 323, 504
AcsI RAATTY 1 cut(s) 899
AcuI CTGAAG 1 cut(s) 569
AfaI GTAC 1 cut(s) 747
AfiI CCNNNNNNNGG 3 cut(s) 248, 414, 983
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 6 cut(s) 9, 76, 308, 339, 670, 710
AjnI CCWGG 1 cut(s) 223
AleI CACNNNNGTG 1 cut(s) 504
AluBI AGCT 5 cut(s) 343, 386, 878, 990, 1001
AluI AGCT 5 cut(s) 343, 386, 878, 990, 1001
Alw21I GWGCWC 3 cut(s) 422, 520, 855
Alw26I GTCTC 1 cut(s) 353
Alw44I GTGCAC 2 cut(s) 516, 851
AlwI GGATC 2 cut(s) 323, 504
Aor13HI TCCGGA 1 cut(s) 807
AoxI GGCC 2 cut(s) 250, 536
ApaLI GTGCAC 2 cut(s) 516, 851
ApeKI GCWGC 2 cut(s) 386, 684
ApoI RAATTY 1 cut(s) 899
Asp700I GAANNNNTTC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 1 cut(s) 397
AvaII GGWCC 1 cut(s) 329
BaeGI GKGCMC 3 cut(s) 520, 855, 1063
BbsI GAAGAC 1 cut(s) 361
Bbv12I GWGCWC 3 cut(s) 422, 520, 855
BbvCI CCTCAGC 1 cut(s) 492
BbvI GCAGC 2 cut(s) 373, 696
BccI CCATC 2 cut(s) 407, 841
BciT130I CCWGG 1 cut(s) 225
BclI TGATCA 2 cut(s) 18, 754
BcoDI GTCTC 1 cut(s) 353
BfaI CTAG 2 cut(s) 39, 879
BfmI CTRYAG 1 cut(s) 291
BglII AGATCT 1 cut(s) 916
BisI GCNGC 2 cut(s) 387, 685
BlsI GCNGC 2 cut(s) 388, 686
Bme1390I CCNGG 1 cut(s) 225
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmrFI CCNGG 1 cut(s) 225
BmsI GCATC 4 cut(s) 267, 588, 621, 813
BoxI GACNNNNGTC 1 cut(s) 1001
BpiI GAAGAC 1 cut(s) 361
BpmI CTGGAG 1 cut(s) 207
Bpu10I CCTNAGC 2 cut(s) 492, 800
BpuEI CTTGAG 3 cut(s) 553, 614, 674
BsaJI CCNNGG 1 cut(s) 376
BsaWI WCCGGW 1 cut(s) 807
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 3 cut(s) 248, 414, 983
Bse118I RCCGGY 1 cut(s) 652
Bse1I ACTGG 4 cut(s) 332, 408, 608, 957
BseAI TCCGGA 1 cut(s) 807
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 376
BseGI GGATG 3 cut(s) 372, 577, 804
BseLI CCNNNNNNNGG 3 cut(s) 248, 414, 983
BseMII CTCAG 4 cut(s) 471, 483, 531, 953
BseNI ACTGG 4 cut(s) 332, 408, 608, 957
BseSI GKGCMC 3 cut(s) 520, 855, 1063
BseXI GCAGC 2 cut(s) 373, 696
Bsh1236I CGCG 1 cut(s) 735
BshFI GGCC 2 cut(s) 252, 538
BsiHKAI GWGCWC 3 cut(s) 422, 520, 855
BsiSI CCGG 2 cut(s) 653, 808
BslI CCNNNNNNNGG 3 cut(s) 248, 414, 983
BsmAI GTCTC 1 cut(s) 353
BsnI GGCC 2 cut(s) 252, 538
Bsp1286I GDGCHC 4 cut(s) 422, 520, 855, 1063
Bsp13I TCCGGA 1 cut(s) 807
Bsp143I GATC 5 cut(s) 18, 315, 496, 754, 916
Bsp19I CCATGG 1 cut(s) 376
BspACI CCGC 1 cut(s) 893
BspANI GGCC 2 cut(s) 252, 538
BspCNI CTCAG 4 cut(s) 472, 484, 532, 954
BspEI TCCGGA 1 cut(s) 807
BspFNI CGCG 1 cut(s) 735
BspMAI CTGCAG 1 cut(s) 295
BspPI GGATC 2 cut(s) 323, 504
BsrFI RCCGGY 1 cut(s) 652
BsrI ACTGG 4 cut(s) 332, 408, 608, 957
BssAI RCCGGY 1 cut(s) 652
BssECI CCNNGG 1 cut(s) 376
BssMI GATC 5 cut(s) 18, 315, 496, 754, 916
BssT1I CCWWGG 1 cut(s) 376
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 367
Bst6I CTCTTC 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 282, 359, 697
BstDEI CTNAG 6 cut(s) 344, 480, 492, 540, 800, 962
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 3 cut(s) 372, 577, 804
BstFNI CGCG 1 cut(s) 735
BstKTI GATC 5 cut(s) 21, 318, 499, 757, 919
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 5 cut(s) 18, 315, 496, 754, 916
BstMWI GCNNNNNNNGC 4 cut(s) 281, 290, 670, 801
BstNI CCWGG 1 cut(s) 225
BstPAI GACNNNNGTC 1 cut(s) 1001
BstSCI CCNGG 1 cut(s) 223
BstSFI CTRYAG 1 cut(s) 291
BstSLI GKGCMC 3 cut(s) 520, 855, 1063
BstUI CGCG 1 cut(s) 735
BstV1I GCAGC 2 cut(s) 373, 696
BstV2I GAAGAC 1 cut(s) 361
BstX2I RGATCY 1 cut(s) 916
BstYI RGATCY 1 cut(s) 916
BsuRI GGCC 2 cut(s) 252, 538
BtgI CCRYGG 1 cut(s) 376
BtgZI GCGATG 1 cut(s) 290
BtsCI GGATG 3 cut(s) 372, 577, 804
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 6 cut(s) 288, 415, 545, 601, 654, 950
Cac8I GCNNGC 3 cut(s) 282, 359, 697
Cfr10I RCCGGY 1 cut(s) 652
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 746
CspCI CAANNNNNGTGG 4 cut(s) 542, 577, 623, 658
CviAII CATG 6 cut(s) 133, 377, 608, 862, 909, 939
CviQI GTAC 1 cut(s) 746
DdeI CTNAG 6 cut(s) 344, 480, 492, 540, 800, 962
DpnI GATC 5 cut(s) 20, 317, 498, 756, 918
DpnII GATC 5 cut(s) 18, 315, 496, 754, 916
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Eco130I CCWWGG 1 cut(s) 376
Eco147I AGGCCT 1 cut(s) 538
Eco47I GGWCC 1 cut(s) 329
Eco57I CTGAAG 1 cut(s) 569
EcoRII CCWGG 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 376
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 6 cut(s) 136, 380, 611, 865, 912, 942
FatI CATG 6 cut(s) 132, 376, 607, 861, 908, 938
FauI CCCGC 1 cut(s) 886
FbaI TGATCA 2 cut(s) 18, 754
Fnu4HI GCNGC 2 cut(s) 387, 685
FokI GGATG 3 cut(s) 359, 584, 791
Fsp4HI GCNGC 2 cut(s) 387, 685
FspBI CTAG 2 cut(s) 39, 879
GluI GCNGC 2 cut(s) 387, 685
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 2 cut(s) 252, 538
HapII CCGG 2 cut(s) 653, 808
Hin1II CATG 6 cut(s) 136, 380, 611, 865, 912, 942
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HinfI GANTC 5 cut(s) 42, 443, 542, 970, 1026
HpaII CCGG 2 cut(s) 653, 808
HphI GGTGA 1 cut(s) 397
Hpy166II GTNNAC 3 cut(s) 518, 658, 853
Hpy188I TCNGA 6 cut(s) 211, 272, 320, 481, 512, 963
Hpy188III TCNNGA 4 cut(s) 532, 583, 808, 824
Hpy8I GTNNAC 3 cut(s) 518, 658, 853
HpyAV CCTTC 4 cut(s) 191, 676, 812, 987
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4IV ACGT 1 cut(s) 505
HpyCH4V TGCA 8 cut(s) 92, 293, 389, 518, 601, 634, 853, 865
HpyF10VI GCNNNNNNNGC 4 cut(s) 281, 290, 670, 801
HpyF3I CTNAG 6 cut(s) 344, 480, 492, 540, 800, 962
HpySE526I ACGT 1 cut(s) 505
Hsp92II CATG 6 cut(s) 136, 380, 611, 865, 912, 942
Kpn2I TCCGGA 1 cut(s) 807
Ksp22I TGATCA 2 cut(s) 18, 754
Kzo9I GATC 5 cut(s) 18, 315, 496, 754, 916
LmnI GCTCC 1 cut(s) 417
Lsp1109I GCAGC 2 cut(s) 373, 696
LweI GCATC 4 cut(s) 267, 588, 621, 813
MaeI CTAG 2 cut(s) 39, 879
MaeII ACGT 1 cut(s) 505
MaeIII GTNAC 3 cut(s) 543, 622, 674
MalI GATC 5 cut(s) 20, 317, 498, 756, 918
MboI GATC 5 cut(s) 18, 315, 496, 754, 916
MboII GAAGA 6 cut(s) 154, 361, 587, 839, 881, 926
MflI RGATCY 1 cut(s) 916
MhlI GDGCHC 4 cut(s) 422, 520, 855, 1063
MluCI AATT 4 cut(s) 153, 830, 887, 899
MlyI GAGTC 2 cut(s) 452, 551
MmeI TCCRAC 1 cut(s) 822
MroI TCCGGA 1 cut(s) 807
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 3 cut(s) 485, 833, 905
MslI CAYNNNNRTG 3 cut(s) 504, 606, 1066
MspA1I CMGCKG 1 cut(s) 1001
MspI CCGG 2 cut(s) 653, 808
MspR9I CCNGG 1 cut(s) 225
MvaI CCWGG 1 cut(s) 225
MvnI CGCG 1 cut(s) 735
MwoI GCNNNNNNNGC 4 cut(s) 281, 290, 670, 801
NcoI CCATGG 1 cut(s) 376
NdeII GATC 5 cut(s) 18, 315, 496, 754, 916
NlaIII CATG 6 cut(s) 136, 380, 611, 865, 912, 942
NmuCI GTSAC 1 cut(s) 543
OliI CACNNNNGTG 1 cut(s) 504
PceI AGGCCT 1 cut(s) 538
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 3 cut(s) 42, 970, 1026
PkrI GCNGC 2 cut(s) 388, 686
PleI GAGTC 2 cut(s) 451, 550
PpsI GAGTC 2 cut(s) 451, 550
PshAI GACNNNNGTC 1 cut(s) 1001
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspPI GGNCC 1 cut(s) 329
PstI CTGCAG 1 cut(s) 295
PsuI RGATCY 1 cut(s) 916
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 1 cut(s) 747
RsaNI GTAC 1 cut(s) 746
RseI CAYNNNNRTG 3 cut(s) 504, 606, 1066
SaqAI TTAA 3 cut(s) 485, 833, 905
SatI GCNGC 2 cut(s) 387, 685
Sau3AI GATC 5 cut(s) 18, 315, 496, 754, 916
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 2 cut(s) 452, 551
ScrFI CCNGG 1 cut(s) 225
SduI GDGCHC 4 cut(s) 422, 520, 855, 1063
SfaNI GCATC 4 cut(s) 267, 588, 621, 813
SfcI CTRYAG 1 cut(s) 291
SinI GGWCC 1 cut(s) 329
SmiMI CAYNNNNRTG 3 cut(s) 504, 606, 1066
SmlI CTYRAG 3 cut(s) 532, 593, 689
SmoI CTYRAG 3 cut(s) 532, 593, 689
Sse9I AATT 4 cut(s) 153, 830, 887, 899
SseBI AGGCCT 1 cut(s) 538
SsiI CCGC 1 cut(s) 893
SspMI CTAG 2 cut(s) 39, 879
StuI AGGCCT 1 cut(s) 538
StyD4I CCNGG 1 cut(s) 223
StyI CCWWGG 1 cut(s) 376
TaaI ACNGT 1 cut(s) 367
TaiI ACGT 1 cut(s) 508
TasI AATT 4 cut(s) 153, 830, 887, 899
TfiI GAWTC 3 cut(s) 42, 970, 1026
Tru1I TTAA 3 cut(s) 485, 833, 905
Tru9I TTAA 3 cut(s) 485, 833, 905
TscAI CASTG 6 cut(s) 295, 415, 552, 608, 654, 957
TseFI GTSAC 1 cut(s) 543
TseI GCWGC 2 cut(s) 386, 684
Tsp45I GTSAC 1 cut(s) 543
TspDTI ATGAA 4 cut(s) 361, 588, 831, 944
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 6 cut(s) 295, 415, 552, 608, 654, 957
VneI GTGCAC 2 cut(s) 516, 851
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 1 cut(s) 899
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 2 cut(s) 39, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.