Rroxscaffold_4G00285140

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000704
Physical Location & Seq
Reverse (-)
6564777 .. 6570035
5259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00285140.1

Sequence Viewer

Length: 1269 bp
ATGTCGTCGCCGTCTCGCCGAGAATGGGAGAGCTATAGCGTCGATTCTTCTTCTTCAATGAAGCCGGTAACTATTGCGTCCCCGTTTAAAACATCACTAGGGAAGAAACTTCCACCAGGGTCATTTGGTATTCCGTTAATTGGAGAGTCCATAAGCTTCATCCGAGCACAAAAACAGGACGGATCCGATGAGTGGATTCGGTCCCGTATCCGCAAGTATGGACCCGTCTTCAAGACCTCGCTCATGGGCTCTAAAACGGTGGTTATAACTGGCCAAACTGGAAACCGGTTTGTGTTTGGTGGAGGTGACACTGGTATTGGAGGCAACCAAGTAGCAACTGTAGCCAGAATTCTCGGAAAGCACAGCATATTTGAGATTTCTGGGTTTAGGCACAAGCTTGTGAGGAGTGCCATAACCAACTTCCTAAAGCCAGAAAACATTCAAAGAATTGTAGGCGAAATCGATTTGTTAGTCAAAGACCAAATTTTCAAGGAACTCGATGGCAAGGATGTAGTACAATTAGTGCCTTTGGTGAAGAGATTAACGTTCAAGGTTACTTGCAGTTTGTTTTTTGGACTACCAGAAGGCAAAGACCAAGATACATTGTTTGAAGACTTTACTATTGCCATCAAGGGTCTGTGGGCAATTCCATTAAACCTTCTAGGCACTCAATTTTACAAAGCAGTGCAAGCACGTGGCAGGATAGCCAAGCTCCTCACCAAGCTCATGAAGGAAAAGAAGAATAAGGCAACAAAAATGACCCCCCAGAACAATGACATCATCTCAGCCCTGCTTCTTCTGAGAGATGAAGTTGGTAAGCCTCTGCAAGAGCAAGAGATTGTTGATAATCTCATCACTGTGATAGTTGCTAGCCATGACACCACCTCAATTCTCCTGAGCCTCCTTTTAAGGCATCTTTCTACAGATTCCAAGATTTTCAGTGAGGTCCTTCAAGGACATGCAGAGCAAAGAGAAGTAGTTAAAGCTGTTGAAGGAAGTGAAAGAAAGGTTATTACATGGAATGAAATCCAAATGATGAAGCACACATGGAGAGTGGCCCAAGAGCTCATGAGGATGACTCCCCCGCTTTTGGTAACTTCAAATGTGCATGGAGAGATACTAACTTCGATGGCTATGATATCCCCAAGGGATGGAAGGTGCATGCATGTTACCCTAACTCTAAATCTAACCCAGCTTCAAATTCAGTATTTTGAATTGAACTTGATCGAGCATGGAACTTGGCTTGGATACAATTTTATAATTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

422

Amino Acids

47.32

Weight (kDa)

9.59

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 38 - 364 5.4e-25 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 266, 1259
AccB7I CCANNNNNTGG 1 cut(s) 1151
AciI CCGC 2 cut(s) 211, 1085
AclI AACGTT 1 cut(s) 545
AclWI GGATC 2 cut(s) 177, 190
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 3 cut(s) 348, 483, 1200
AcvI CACGTG 1 cut(s) 695
AfaI GTAC 1 cut(s) 516
AfiI CCNNNNNNNGG 5 cut(s) 25, 140, 192, 1090, 1151
AgeI ACCGGT 1 cut(s) 285
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 8 cut(s) 33, 156, 397, 712, 724, 986, 1066, 1195
AluI AGCT 8 cut(s) 33, 156, 397, 712, 724, 986, 1066, 1195
Alw21I GWGCWC 2 cut(s) 169, 1068
Alw26I GTCTC 1 cut(s) 18
AlwI GGATC 2 cut(s) 177, 190
AoxI GGCC 2 cut(s) 271, 1056
ApoI RAATTY 3 cut(s) 348, 483, 1200
AsiGI ACCGGT 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 4 cut(s) 201, 221, 946, 1057
AsuHPI GGTGA 3 cut(s) 317, 544, 709
AsuNHI GCTAGC 1 cut(s) 869
AvaII GGWCC 3 cut(s) 201, 221, 946
BalI TGGCCA 1 cut(s) 273
BamHI GGATCC 1 cut(s) 182
BanII GRGCYC 2 cut(s) 251, 1068
BbrPI CACGTG 1 cut(s) 695
BbsI GAAGAC 2 cut(s) 220, 618
Bbv12I GWGCWC 2 cut(s) 169, 1068
BccI CCATC 4 cut(s) 494, 635, 1123, 1145
BciT130I CCWGG 1 cut(s) 117
BciVI GTATCC 2 cut(s) 218, 1241
BcoDI GTCTC 1 cut(s) 18
BfaI CTAG 3 cut(s) 98, 662, 870
BfmI CTRYAG 3 cut(s) 34, 339, 921
BfuI GTATCC 2 cut(s) 218, 1241
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 3 cut(s) 201, 221, 946
BmgT120I GGNCC 4 cut(s) 201, 221, 946, 1057
BmiI GGNNCC 3 cut(s) 184, 203, 223
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 922
BmtI GCTAGC 1 cut(s) 873
BpiI GAAGAC 2 cut(s) 220, 618
BplI GAGNNNNNCTC 2 cut(s) 1063, 1095
Bpu10I CCTNAGC 1 cut(s) 896
Bsa29I ATCGAT 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 695
BsaJI CCNNGG 2 cut(s) 116, 1145
BsaWI WCCGGW 1 cut(s) 285
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 140, 192, 1090, 1151
Bse118I RCCGGY 2 cut(s) 64, 285
Bse1I ACTGG 3 cut(s) 274, 283, 316
BseBI CCWGG 1 cut(s) 117
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 116, 1145
BseGI GGATG 4 cut(s) 159, 514, 1080, 1156
BseLI CCNNNNNNNGG 5 cut(s) 25, 140, 192, 1090, 1151
BseMII CTCAG 3 cut(s) 791, 798, 887
BseNI ACTGG 3 cut(s) 274, 283, 316
BseRI GAGGAG 2 cut(s) 418, 704
BseYI CCCAGC 1 cut(s) 1191
BshFI GGCC 2 cut(s) 273, 1058
BshTI ACCGGT 1 cut(s) 285
BshVI ATCGAT 1 cut(s) 462
BsiHKAI GWGCWC 2 cut(s) 169, 1068
BsiSI CCGG 2 cut(s) 65, 286
BslFI GGGAC 2 cut(s) 64, 187
BslI CCNNNNNNNGG 5 cut(s) 25, 140, 192, 1090, 1151
BsmAI GTCTC 1 cut(s) 18
BsmBI CGTCTC 1 cut(s) 18
BsmFI GGGAC 2 cut(s) 64, 187
BsnI GGCC 2 cut(s) 273, 1058
Bsp1286I GDGCHC 3 cut(s) 169, 251, 1068
Bsp143I GATC 2 cut(s) 182, 1224
BspACI CCGC 2 cut(s) 211, 1085
BspANI GGCC 2 cut(s) 273, 1058
BspCNI CTCAG 3 cut(s) 792, 797, 888
BspDI ATCGAT 1 cut(s) 462
BspHI TCATGA 2 cut(s) 726, 1068
BspLI GGNNCC 3 cut(s) 184, 203, 223
BspOI GCTAGC 1 cut(s) 873
BspPI GGATC 2 cut(s) 177, 190
BsrFI RCCGGY 2 cut(s) 64, 285
BsrI ACTGG 3 cut(s) 274, 283, 316
BssAI RCCGGY 2 cut(s) 64, 285
BssECI CCNNGG 2 cut(s) 116, 1145
BssMI GATC 2 cut(s) 182, 1224
BssT1I CCWWGG 1 cut(s) 1145
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 3 cut(s) 259, 340, 859
Bst6I CTCTTC 1 cut(s) 530
BstBAI YACGTR 1 cut(s) 695
BstC8I GCNNGC 3 cut(s) 690, 871, 1163
BstDEI CTNAG 3 cut(s) 784, 800, 896
BstF5I GGATG 4 cut(s) 159, 514, 1080, 1156
BstKTI GATC 2 cut(s) 185, 1227
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 2 cut(s) 182, 1224
BstMWI GCNNNNNNNGC 2 cut(s) 341, 689
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 3 cut(s) 962, 1165, 1169
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 3 cut(s) 34, 339, 921
BstV2I GAAGAC 2 cut(s) 220, 618
BstX2I RGATCY 1 cut(s) 182
BstYI RGATCY 1 cut(s) 182
Bsu15I ATCGAT 1 cut(s) 462
BsuI GTATCC 2 cut(s) 218, 1241
BsuRI GGCC 2 cut(s) 273, 1058
BsuTUI ATCGAT 1 cut(s) 462
BtsCI GGATG 4 cut(s) 159, 514, 1080, 1156
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 4 cut(s) 309, 690, 855, 946
Cac8I GCNNGC 3 cut(s) 690, 871, 1163
CciI TCATGA 2 cut(s) 726, 1068
Cfr10I RCCGGY 2 cut(s) 64, 285
Cfr13I GGNCC 4 cut(s) 201, 221, 946, 1057
ClaI ATCGAT 1 cut(s) 462
CseI GACGC 2 cut(s) 28, 66
Csp6I GTAC 1 cut(s) 515
CspAI ACCGGT 1 cut(s) 285
CviQI GTAC 1 cut(s) 515
DdeI CTNAG 3 cut(s) 784, 800, 896
DpnI GATC 2 cut(s) 184, 1226
DpnII GATC 2 cut(s) 182, 1224
DraI TTTAAA 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 530
EarI CTCTTC 1 cut(s) 530
Ecl136II GAGCTC 1 cut(s) 1066
Eco130I CCWWGG 1 cut(s) 1145
Eco24I GRGCYC 2 cut(s) 251, 1068
Eco32I GATATC 1 cut(s) 1140
Eco47I GGWCC 3 cut(s) 201, 221, 946
Eco53kI GAGCTC 1 cut(s) 1066
Eco72I CACGTG 1 cut(s) 695
EcoICRI GAGCTC 1 cut(s) 1066
EcoO109I RGGNCCY 1 cut(s) 946
EcoRI GAATTC 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 115
EcoRV GATATC 1 cut(s) 1140
EcoT14I CCWWGG 1 cut(s) 1145
EcoT22I ATGCAT 1 cut(s) 1167
EcoT38I GRGCYC 2 cut(s) 251, 1068
ErhI CCWWGG 1 cut(s) 1145
Esp3I CGTCTC 1 cut(s) 18
FaqI GGGAC 2 cut(s) 64, 187
FauI CCCGC 1 cut(s) 1092
FokI GGATG 4 cut(s) 146, 521, 1087, 1163
FriOI GRGCYC 2 cut(s) 251, 1068
FspBI CTAG 3 cut(s) 98, 662, 870
GsaI CCCAGC 1 cut(s) 1195
HaeIII GGCC 2 cut(s) 273, 1058
HapII CCGG 2 cut(s) 65, 286
HgaI GACGC 2 cut(s) 28, 66
HindIII AAGCTT 2 cut(s) 154, 395
HinfI GANTC 5 cut(s) 44, 146, 196, 926, 1078
HpaII CCGG 2 cut(s) 65, 286
HphI GGTGA 3 cut(s) 317, 544, 709
Hpy188I TCNGA 4 cut(s) 164, 187, 356, 801
Hpy188III TCNNGA 4 cut(s) 232, 727, 895, 1069
Hpy99I CGWCG 2 cut(s) 10, 44
HpyAV CCTTC 6 cut(s) 578, 668, 724, 959, 986, 1149
HpyCH4III ACNGT 3 cut(s) 259, 340, 859
HpyCH4IV ACGT 2 cut(s) 545, 694
HpyCH4V TGCA 7 cut(s) 561, 688, 826, 962, 1108, 1161, 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 689
HpyF3I CTNAG 3 cut(s) 784, 800, 896
HpySE526I ACGT 2 cut(s) 545, 694
Kzo9I GATC 2 cut(s) 182, 1224
LmnI GCTCC 1 cut(s) 717
LweI GCATC 1 cut(s) 922
MaeI CTAG 3 cut(s) 98, 662, 870
MaeII ACGT 2 cut(s) 545, 694
MaeIII GTNAC 5 cut(s) 67, 305, 553, 1093, 1168
MalI GATC 2 cut(s) 184, 1226
MboI GATC 2 cut(s) 182, 1224
MboII GAAGA 9 cut(s) 39, 42, 45, 115, 220, 547, 623, 751, 788
MflI RGATCY 1 cut(s) 182
MhlI GDGCHC 3 cut(s) 169, 251, 1068
MlsI TGGCCA 1 cut(s) 273
MluNI TGGCCA 1 cut(s) 273
MlyI GAGTC 2 cut(s) 155, 1072
Mox20I TGGCCA 1 cut(s) 273
Mph1103I ATGCAT 1 cut(s) 1167
MroXI GAANNNNTTC 1 cut(s) 438
MscI TGGCCA 1 cut(s) 273
MseI TTAA 7 cut(s) 87, 137, 542, 653, 908, 981, 1263
MslI CAYNNNNRTG 2 cut(s) 857, 1073
Msp20I TGGCCA 1 cut(s) 273
MspI CCGG 2 cut(s) 65, 286
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 2 cut(s) 341, 689
NdeII GATC 2 cut(s) 182, 1224
NheI GCTAGC 1 cut(s) 869
NlaIV GGNNCC 3 cut(s) 184, 203, 223
NmeAIII GCCGAG 1 cut(s) 44
NmuCI GTSAC 1 cut(s) 305
NsiI ATGCAT 1 cut(s) 1167
NspI RCATGY 3 cut(s) 962, 1165, 1169
PaeI GCATGC 1 cut(s) 1165
PagI TCATGA 2 cut(s) 726, 1068
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 3 cut(s) 44, 196, 926
PflMI CCANNNNNTGG 1 cut(s) 1151
PinAI ACCGGT 1 cut(s) 285
PleI GAGTC 2 cut(s) 154, 1072
PmaCI CACGTG 1 cut(s) 695
PmlI CACGTG 1 cut(s) 695
PpsI GAGTC 2 cut(s) 154, 1072
Ppu21I YACGTR 1 cut(s) 695
PpuMI RGGWCCY 1 cut(s) 946
PsiI TTATAA 2 cut(s) 266, 1259
Psp124BI GAGCTC 1 cut(s) 1068
Psp1406I AACGTT 1 cut(s) 545
Psp5II RGGWCCY 1 cut(s) 946
Psp6I CCWGG 1 cut(s) 115
PspCI CACGTG 1 cut(s) 695
PspFI CCCAGC 1 cut(s) 1191
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 184, 203, 223
PspPI GGNCC 4 cut(s) 201, 221, 946, 1057
PspPPI RGGWCCY 1 cut(s) 946
PsuI RGATCY 1 cut(s) 182
RsaI GTAC 1 cut(s) 516
RsaNI GTAC 1 cut(s) 515
RseI CAYNNNNRTG 2 cut(s) 857, 1073
SacI GAGCTC 1 cut(s) 1068
SaqAI TTAA 7 cut(s) 87, 137, 542, 653, 908, 981, 1263
Sau3AI GATC 2 cut(s) 182, 1224
Sau96I GGNCC 4 cut(s) 201, 221, 946, 1057
SchI GAGTC 2 cut(s) 155, 1072
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 3 cut(s) 169, 251, 1068
SfaNI GCATC 1 cut(s) 922
SfcI CTRYAG 3 cut(s) 34, 339, 921
SinI GGWCC 3 cut(s) 201, 221, 946
SmiMI CAYNNNNRTG 2 cut(s) 857, 1073
SphI GCATGC 1 cut(s) 1165
SsiI CCGC 2 cut(s) 211, 1085
SspMI CTAG 3 cut(s) 98, 662, 870
SstI GAGCTC 1 cut(s) 1068
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 1145
TaaI ACNGT 3 cut(s) 259, 340, 859
TaiI ACGT 2 cut(s) 548, 697
TaqI TCGA 5 cut(s) 42, 462, 498, 1127, 1227
TaqII GACCGA 1 cut(s) 189
TatI WGTACW 1 cut(s) 514
TfiI GAWTC 3 cut(s) 44, 196, 926
Tru1I TTAA 7 cut(s) 87, 137, 542, 653, 908, 981, 1263
Tru9I TTAA 7 cut(s) 87, 137, 542, 653, 908, 981, 1263
TscAI CASTG 4 cut(s) 316, 690, 862, 946
TseFI GTSAC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 6 cut(s) 74, 148, 743, 822, 1038, 1052
TspGWI ACGGA 2 cut(s) 123, 195
TspRI CASTG 4 cut(s) 316, 690, 862, 946
Van91I CCANNNNNTGG 1 cut(s) 1151
VpaK11BI GGWCC 3 cut(s) 201, 221, 946
XapI RAATTY 3 cut(s) 348, 483, 1200
XceI RCATGY 3 cut(s) 962, 1165, 1169
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 3 cut(s) 98, 662, 870
Zsp2I ATGCAT 1 cut(s) 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.