Rw5G024580

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000704
Physical Location & Seq
Reverse (-)
34920433 .. 34922427
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G024580.1

Sequence Viewer

Length: 1419 bp
ATGAACTCCCTTTTCGCAGTTTTCCTCTTCCTCATTCCCATATTCCTTCTCCTTACGAGAAGAAAAAAGTCATCCGATAGACTTCCGCCGGGATCATTAGGATTACCCCTGATAGGCCAGAGCCTTGGTCTTCTCCGAGCCATGCGTGCCAACACTGCTGAGAAATGGCTTGAACAAAGGATAAAAAAATATGGTCCAGTTTCGAAGCTAAGTCTCTTTGGCAAGCCAACAGTCTTCATCCACGGACAAGCTGCAAACAAGTTTGTATTCACCAGTGATGGGAGCACCATTTCCAGCCAACAGACTGAGTCTATTCGTAGGATTTTGGGGGAACGGAGTATATTTGAGCTGAGTGCCGAGGATCACAAGCGTGTAAGGAGTGCTCTTATGCTGTTTTTAAAGCCCGAGTCACTGAAGCAATATGTGGGGAAAATGGATGAAGAAATCAGGGAGCACCTTGAAATGCATTGGCATGGAAAACAACAAGTAACAGTCTTGCCCCTGATGAAGAACCTCACGTTCAACATAATATGCTCTCTACTTTTTGGGCTTGAACGAGGAACCCGCAGAGACGAATTCGTGGAGTGTTTCCAAACAATGATAGAAGGAATGTGGTCAGTGCCGGTTAACTTTCCCTTCACGCGTTACAACAGCAGCCTCAAGGCAAGCAAGAGGGTTCAAAACATGATCAAGCAACTTATACGTGAAAAGAGGGTAGAGCTTCAGCAAAAAAAAGCTTCTCCACAACAAGACCTCATCACTTGCTTCCTCAGCATCCGAAATGATGAAGGCAAAGAAGAATTAACAGAATTGGAGATGGTGCACAATGTCATGGTAGTCATGGTTGCAGGACATGACACTTCATCTGTTCTGATTACGTTCCTTTTGCGGCTTCTAGCTAATGAACCAGTTGTTTATGCATCCCTTCTTCAAGAGCAGGAAGAAATAGCTAGAAGCAAATCGGCAGGAGAGTTTCTAACATGGGAAGACCTTGCCAAAATGAAATACACATGGAGAGTAGCACAGGAAACACTGAGGATGACTCCTCCTGTCTTTGGTGGCTTCAGGAATGCTATCAAAGATATTGAGTTCGGTGGATTCCTCATTCCTAAAGGGTGGCAGATATTCTGGGTTGCTCCTATGACCCACATGGACGATAGCATATTCCCAGAGCCATCAAAGTTTGATCCAAGTAGGTTCAACAAGCAAGCATCAGTTCCGCCCTACTGCTTTATACCATTTGGAGGGGGACCTCGAGTATGTCCAGGATATGAGTTTGCCAGGATCGAAACCCTTATTGCAATGCACCATATTGTAACTCAATTCACCTGGAAGCTAAATGCAGACAATCACTTCAGTAGGGATCCAATGCCAGTCCCTACTGGAGGACTTCCTATTGAAATCATGCCTAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

472

Amino Acids

54.08

Weight (kDa)

9.27

Isoelectric Point (pI)

58.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 29 - 450 2.6e-77 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 643
AciI CCGC 4 cut(s) 86, 565, 889, 1220
AclWI GGATC 6 cut(s) 100, 369, 1181, 1292, 1358, 1371
AcsI RAATTY 1 cut(s) 575
AcuI CTGAAG 4 cut(s) 434, 707, 1048, 1339
AfiI CCNNNNNNNGG 5 cut(s) 113, 279, 1055, 1244, 1385
AflIII ACRYGT 1 cut(s) 641
AgsI TTSAA 8 cut(s) 173, 461, 523, 554, 680, 932, 1201, 1400
AjnI CCWGG 3 cut(s) 1264, 1280, 1328
AleI CACNNNNGTG 1 cut(s) 369
AluBI AGCT 8 cut(s) 208, 251, 349, 721, 737, 899, 950, 1336
AluI AGCT 8 cut(s) 208, 251, 349, 721, 737, 899, 950, 1336
Alw21I GWGCWC 4 cut(s) 287, 385, 456, 825
Alw26I GTCTC 2 cut(s) 218, 564
Alw44I GTGCAC 1 cut(s) 821
AlwI GGATC 6 cut(s) 100, 369, 1181, 1292, 1358, 1371
Ama87I CYCGRG 2 cut(s) 404, 1254
AoxI GGCC 1 cut(s) 115
ApaLI GTGCAC 1 cut(s) 821
ApeKI GCWGC 2 cut(s) 251, 654
ApoI RAATTY 1 cut(s) 575
AspS9I GGNCC 2 cut(s) 194, 1250
AsuC2I CCSGG 1 cut(s) 90
AsuHPI GGTGA 2 cut(s) 262, 1318
AsuII TTCGAA 1 cut(s) 203
AvaI CYCGRG 2 cut(s) 404, 1254
AvaII GGWCC 2 cut(s) 194, 1250
BaeGI GKGCMC 1 cut(s) 825
BamHI GGATCC 1 cut(s) 1363
BbsI GAAGAC 3 cut(s) 122, 226, 993
Bbv12I GWGCWC 4 cut(s) 287, 385, 456, 825
BbvCI CCTCAGC 1 cut(s) 770
BbvI GCAGC 2 cut(s) 238, 666
BccI CCATC 3 cut(s) 272, 811, 1183
BciT130I CCWGG 3 cut(s) 1266, 1282, 1330
BclI TGATCA 1 cut(s) 687
BcnI CCSGG 1 cut(s) 90
BcoDI GTCTC 2 cut(s) 218, 564
BfaI CTAG 3 cut(s) 896, 951, 1410
BisI GCNGC 3 cut(s) 252, 655, 890
BlsI GCNGC 3 cut(s) 253, 656, 891
Bme1390I CCNGG 4 cut(s) 90, 1266, 1282, 1330
Bme18I GGWCC 2 cut(s) 194, 1250
BmeT110I CYCGRG 2 cut(s) 404, 1254
BmgT120I GGNCC 2 cut(s) 194, 1250
BmiI GGNNCC 3 cut(s) 562, 1251, 1365
BmrFI CCNGG 4 cut(s) 90, 1266, 1282, 1330
BmsI GCATC 3 cut(s) 783, 929, 1220
BpiI GAAGAC 3 cut(s) 122, 226, 993
BplI GAGNNNNNCTC 2 cut(s) 1027, 1059
BpmI CTGGAG 1 cut(s) 1404
Bpu10I CCTNAGC 1 cut(s) 770
Bpu14I TTCGAA 1 cut(s) 203
BpuEI CTTGAG 1 cut(s) 644
BpuMI CCSGG 1 cut(s) 90
BsaAI YACGTR 1 cut(s) 704
BsaJI CCNNGG 3 cut(s) 124, 241, 357
Bsc4I CCNNNNNNNGG 5 cut(s) 113, 279, 1055, 1244, 1385
Bse118I RCCGGY 1 cut(s) 622
Bse1I ACTGG 5 cut(s) 197, 273, 908, 1373, 1387
Bse3DI GCAATG 1 cut(s) 1308
BseBI CCWGG 3 cut(s) 1266, 1282, 1330
BseDI CCNNGG 3 cut(s) 124, 241, 357
BseGI GGATG 6 cut(s) 71, 237, 442, 774, 920, 1044
BseLI CCNNNNNNNGG 5 cut(s) 113, 279, 1055, 1244, 1385
BseMI GCAATG 1 cut(s) 1308
BseMII CTCAG 5 cut(s) 150, 297, 341, 784, 1025
BseNI ACTGG 5 cut(s) 197, 273, 908, 1373, 1387
BseRI GAGGAG 1 cut(s) 1035
BseSI GKGCMC 1 cut(s) 825
BseXI GCAGC 2 cut(s) 238, 666
Bsh1236I CGCG 1 cut(s) 643
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 4 cut(s) 287, 385, 456, 825
BsiHKCI CYCGRG 2 cut(s) 404, 1254
BsiSI CCGG 2 cut(s) 89, 623
BslFI GGGAC 2 cut(s) 1263, 1361
BslI CCNNNNNNNGG 5 cut(s) 113, 279, 1055, 1244, 1385
BsmAI GTCTC 2 cut(s) 218, 564
BsmBI CGTCTC 1 cut(s) 564
BsmFI GGGAC 2 cut(s) 1263, 1361
BsmI GAATGC 1 cut(s) 1075
BsnI GGCC 1 cut(s) 117
BsoBI CYCGRG 2 cut(s) 404, 1254
Bsp119I TTCGAA 1 cut(s) 203
Bsp1286I GDGCHC 4 cut(s) 287, 385, 456, 825
Bsp143I GATC 6 cut(s) 92, 361, 687, 1186, 1284, 1363
BspACI CCGC 4 cut(s) 86, 565, 889, 1220
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 5 cut(s) 151, 298, 342, 783, 1026
BspFNI CGCG 1 cut(s) 643
BspLI GGNNCC 3 cut(s) 562, 1251, 1365
BspPI GGATC 6 cut(s) 100, 369, 1181, 1292, 1358, 1371
BspT104I TTCGAA 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 1308
BsrFI RCCGGY 1 cut(s) 622
BsrI ACTGG 5 cut(s) 197, 273, 908, 1373, 1387
BssAI RCCGGY 1 cut(s) 622
BssECI CCNNGG 3 cut(s) 124, 241, 357
BssMI GATC 6 cut(s) 92, 361, 687, 1186, 1284, 1363
BssT1I CCWWGG 1 cut(s) 124
Bst2UI CCWGG 3 cut(s) 1266, 1282, 1330
Bst4CI ACNGT 2 cut(s) 232, 493
Bst6I CTCTTC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 704
BstBI TTCGAA 1 cut(s) 203
BstC8I GCNNGC 4 cut(s) 147, 224, 667, 1209
BstDEI CTNAG 6 cut(s) 159, 209, 306, 350, 770, 1034
BstDSI CCRYGG 1 cut(s) 241
BstENI CCTNNNNNAGG 1 cut(s) 1383
BstF5I GGATG 6 cut(s) 71, 237, 442, 774, 920, 1044
BstFNI CGCG 1 cut(s) 643
BstKTI GATC 6 cut(s) 95, 364, 690, 1189, 1287, 1366
BstMAI GTCTC 2 cut(s) 218, 564
BstMBI GATC 6 cut(s) 92, 361, 687, 1186, 1284, 1363
BstMWI GCNNNNNNNGC 3 cut(s) 146, 155, 771
BstNI CCWGG 3 cut(s) 1266, 1282, 1330
BstSCI CCNGG 4 cut(s) 88, 1264, 1280, 1328
BstSLI GKGCMC 1 cut(s) 825
BstUI CGCG 1 cut(s) 643
BstV1I GCAGC 2 cut(s) 238, 666
BstV2I GAAGAC 3 cut(s) 122, 226, 993
BstX2I RGATCY 1 cut(s) 1363
BstXI CCANNNNNNTGG 1 cut(s) 125
BstYI RGATCY 1 cut(s) 1363
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 241
BtsCI GGATG 6 cut(s) 71, 237, 442, 774, 920, 1044
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 5 cut(s) 153, 280, 410, 624, 1031
Cac8I GCNNGC 4 cut(s) 147, 224, 667, 1209
Cfr10I RCCGGY 1 cut(s) 622
Cfr13I GGNCC 2 cut(s) 194, 1250
DdeI CTNAG 6 cut(s) 159, 209, 306, 350, 770, 1034
DpnI GATC 6 cut(s) 94, 363, 689, 1188, 1286, 1365
DpnII GATC 6 cut(s) 92, 361, 687, 1186, 1284, 1363
DraI TTTAAA 1 cut(s) 399
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 2 cut(s) 75, 1209
Eco130I CCWWGG 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 194, 1250
Eco57I CTGAAG 4 cut(s) 434, 707, 1048, 1339
Eco88I CYCGRG 2 cut(s) 404, 1254
EcoNI CCTNNNNNAGG 1 cut(s) 1383
EcoO109I RGGNCCY 1 cut(s) 1250
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 3 cut(s) 1264, 1280, 1328
EcoT14I CCWWGG 1 cut(s) 124
EcoT22I ATGCAT 2 cut(s) 468, 922
ErhI CCWWGG 1 cut(s) 124
Esp3I CGTCTC 1 cut(s) 564
FaqI GGGAC 2 cut(s) 1263, 1361
FauI CCCGC 1 cut(s) 572
FbaI TGATCA 1 cut(s) 687
Fnu4HI GCNGC 3 cut(s) 252, 655, 890
FokI GGATG 6 cut(s) 58, 224, 449, 761, 907, 1051
Fsp4HI GCNGC 3 cut(s) 252, 655, 890
FspBI CTAG 3 cut(s) 896, 951, 1410
GluI GCNGC 3 cut(s) 252, 655, 890
GsuI CTGGAG 1 cut(s) 1404
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 89, 623
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HindIII AAGCTT 1 cut(s) 735
HinfI GANTC 4 cut(s) 308, 407, 1042, 1098
HpaI GTTAAC 1 cut(s) 628
HpaII CCGG 2 cut(s) 89, 623
HphI GGTGA 2 cut(s) 262, 1318
Hpy166II GTNNAC 2 cut(s) 628, 823
Hpy188I TCNGA 4 cut(s) 76, 137, 779, 873
Hpy188III TCNNGA 2 cut(s) 932, 1066
Hpy8I GTNNAC 2 cut(s) 628, 823
HpyAV CCTTC 5 cut(s) 56, 599, 646, 782, 935
HpyCH4III ACNGT 2 cut(s) 232, 493
HpyCH4IV ACGT 3 cut(s) 518, 703, 878
HpyCH4V TGCA 8 cut(s) 254, 466, 823, 848, 920, 1301, 1306, 1343
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 155, 771
HpyF3I CTNAG 6 cut(s) 159, 209, 306, 350, 770, 1034
HpySE526I ACGT 3 cut(s) 518, 703, 878
Ksp22I TGATCA 1 cut(s) 687
KspAI GTTAAC 1 cut(s) 628
Kzo9I GATC 6 cut(s) 92, 361, 687, 1186, 1284, 1363
LmnI GCTCC 3 cut(s) 282, 451, 1141
Lsp1109I GCAGC 2 cut(s) 238, 666
LweI GCATC 3 cut(s) 783, 929, 1220
MaeI CTAG 3 cut(s) 896, 951, 1410
MaeII ACGT 3 cut(s) 518, 703, 878
MaeIII GTNAC 4 cut(s) 408, 487, 644, 1315
MalI GATC 6 cut(s) 94, 363, 689, 1188, 1286, 1365
MboI GATC 6 cut(s) 92, 361, 687, 1186, 1284, 1363
MflI RGATCY 1 cut(s) 1363
MhlI GDGCHC 4 cut(s) 287, 385, 456, 825
MluCI AATT 4 cut(s) 575, 800, 809, 1322
MluI ACGCGT 1 cut(s) 641
MlyI GAGTC 3 cut(s) 317, 416, 1036
Mph1103I ATGCAT 2 cut(s) 468, 922
MseI TTAA 3 cut(s) 398, 627, 803
MslI CAYNNNNRTG 2 cut(s) 369, 471
MspI CCGG 2 cut(s) 89, 623
MspR9I CCNGG 4 cut(s) 90, 1266, 1282, 1330
Mva1269I GAATGC 1 cut(s) 1075
MvaI CCWGG 3 cut(s) 1266, 1282, 1330
MvnI CGCG 1 cut(s) 643
MwoI GCNNNNNNNGC 3 cut(s) 146, 155, 771
NciI CCSGG 1 cut(s) 90
NdeII GATC 6 cut(s) 92, 361, 687, 1186, 1284, 1363
NlaIV GGNNCC 3 cut(s) 562, 1251, 1365
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 408
NsiI ATGCAT 2 cut(s) 468, 922
NspV TTCGAA 1 cut(s) 203
OliI CACNNNNGTG 1 cut(s) 369
PaeR7I CTCGAG 1 cut(s) 1254
PctI GAATGC 1 cut(s) 1075
PfeI GAWTC 1 cut(s) 1098
PflFI GACNNNGTC 1 cut(s) 307
PfoI TCCNGGA 1 cut(s) 1264
PkrI GCNGC 3 cut(s) 253, 656, 891
PleI GAGTC 3 cut(s) 316, 415, 1036
PpsI GAGTC 3 cut(s) 316, 415, 1036
Ppu21I YACGTR 1 cut(s) 704
PpuMI RGGWCCY 1 cut(s) 1250
Psp5II RGGWCCY 1 cut(s) 1250
Psp6I CCWGG 3 cut(s) 1264, 1280, 1328
PspGI CCWGG 3 cut(s) 1264, 1280, 1328
PspN4I GGNNCC 3 cut(s) 562, 1251, 1365
PspPI GGNCC 2 cut(s) 194, 1250
PspPPI RGGWCCY 1 cut(s) 1250
PspXI VCTCGAGB 1 cut(s) 1254
PsuI RGATCY 1 cut(s) 1363
PsyI GACNNNGTC 1 cut(s) 307
RseI CAYNNNNRTG 2 cut(s) 369, 471
SaqAI TTAA 3 cut(s) 398, 627, 803
SatI GCNGC 3 cut(s) 252, 655, 890
Sau3AI GATC 6 cut(s) 92, 361, 687, 1186, 1284, 1363
Sau96I GGNCC 2 cut(s) 194, 1250
SchI GAGTC 3 cut(s) 317, 416, 1036
ScrFI CCNGG 4 cut(s) 90, 1266, 1282, 1330
SduI GDGCHC 4 cut(s) 287, 385, 456, 825
SfaNI GCATC 3 cut(s) 783, 929, 1220
Sfr274I CTCGAG 1 cut(s) 1254
SfuI TTCGAA 1 cut(s) 203
SinI GGWCC 2 cut(s) 194, 1250
SlaI CTCGAG 1 cut(s) 1254
SmiMI CAYNNNNRTG 2 cut(s) 369, 471
SmlI CTYRAG 2 cut(s) 659, 1254
SmoI CTYRAG 2 cut(s) 659, 1254
Sse9I AATT 4 cut(s) 575, 800, 809, 1322
SsiI CCGC 4 cut(s) 86, 565, 889, 1220
SspMI CTAG 3 cut(s) 896, 951, 1410
StyD4I CCNGG 4 cut(s) 88, 1264, 1280, 1328
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 2 cut(s) 232, 493
TaiI ACGT 3 cut(s) 521, 706, 881
TaqI TCGA 3 cut(s) 203, 1255, 1287
TasI AATT 4 cut(s) 575, 800, 809, 1322
TauI GCSGC 1 cut(s) 892
TfiI GAWTC 1 cut(s) 1098
Tru1I TTAA 3 cut(s) 398, 627, 803
Tru9I TTAA 3 cut(s) 398, 627, 803
TscAI CASTG 5 cut(s) 160, 280, 417, 624, 1038
TseFI GTSAC 1 cut(s) 408
TseI GCWGC 2 cut(s) 251, 654
Tsp45I GTSAC 1 cut(s) 408
TspDTI ATGAA 8 cut(s) 17, 226, 453, 521, 801, 852, 918, 1016
TspGWI ACGGA 2 cut(s) 258, 349
TspRI CASTG 5 cut(s) 160, 280, 417, 624, 1038
Tth111I GACNNNGTC 1 cut(s) 307
VneI GTGCAC 1 cut(s) 821
VpaK11BI GGWCC 2 cut(s) 194, 1250
XagI CCTNNNNNAGG 1 cut(s) 1383
XapI RAATTY 1 cut(s) 575
XhoI CTCGAG 1 cut(s) 1254
XspI CTAG 3 cut(s) 896, 951, 1410
Zsp2I ATGCAT 2 cut(s) 468, 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.