pycom03g15500

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000704
Physical Location & Seq
Forward (+)
16744801 .. 16746768
1968 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g15500.3

Sequence Viewer

Length: 1425 bp
ATGAATACCCTTTTCTTCACAGTTTTCCTTTTCCTCATCCCCATCTTGCTTCTCTTTGCAAGGAGAAAAAGATCACCAAAAAGGGTTCCTCCAGGCTCATTAGGACTACCCATAATCGGCCAAAGCCTCGGTCTTCTCCGAGCCATGCGTGCCAACACCGCCGAAAAATGGCTACAACAAAGAATCGAAAAGTACGGCCCAGTTTCAAAGCTAAGTCTCTTTGGCAAACCAACGGTCTTCATTCATGGACCTGCTGCGAACAAGTTCATATTCGCCAGTGATGACAGCACCATTTCCAGTCAACAAGTTGAGTCTATTCGTCTACTTTTGGGTGACCGGAATATACTGGAGCTGAGCGGCGACGATCACAAGCGCGTTAGAAGTGCTCTTATGTTGTTCTTGAAGCCCGAGTCACTCAAGCAGTATGTGGGAAAGATGGACCAAGAAATCAGGAGACACCTTGAGATGCATTGGCATGGCAAGCAACAAATAACTGTCTTGCCCCTGATGAAAAACCTCACATTCAACATAATATGCTCCCTACTTTTTAGGCTTGAACAGGGAACTCGCAGAGATGAACTCTTGGAGTGTTTCCAAAAAATGATAGAAGGAATGTGGTCAGTCCCGGTTAAATTGCCCTTCACGCGTTACAGCCAGAGCCTCAAGGGAAGCAAGAGGGTCCAAGACATAATAAAGGATCTCATATCGGAAAAGAGGATAGAACTTGAGCAAAAAATTGCTTCTCCGCTCCAAGACCTTATCACTTGCTTGCTTAGCAGTCGAAGTGATAACAATGGAAAGGTTATGACTGAAAAGGAGATCCTGCACAATATTATGTTAGTGATGGTCGCAGGTCATGACACTTCATCTGTTGTGCTTACTTTTCTATTGCGGCTTCTCGCTAATGACCCCGTTGTACACGCAACCCTTCTTCAAGAGCAAGAAGAAATAGCTAGAAGCAAATCCTCGGAAGAGTTGACATGGAAAGACCTTGCCAAGATGAAGTACACATGGAGAGTTGTAATGGAAACGATGAGAATAACTCCTCCTATCTTTGGTGGCTTCCGGAGTGCATTGAAAGACATCGAGTTTGATAGATTTCTCATCCCCAAAGGGTGGCAAATATTCTGGGCTATGCCGATGACTCATATGGACGATGACATATTTCCGGAGCCATCCAAGTTTGATCCGAATAGGTTTGAGAACCAAGCATCAACTCCACCTTACAGCTTTGTTCCATTTGGAGGAGGACCTCGAAAATGTCCAGGATATGAGTTTGCCCGGATCGAAATCCTTGTCGCAATTCATTATATGGTAACTCAGTTCACATGGAAGCTATGCGCAGACAACAACTTCAGTAGAGATCCCATGCCAGTACCTACTCAGGGACTACCCATAAAAATCACCCCAAGAAAAGCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

475

Amino Acids

54.48

Weight (kDa)

9.49

Isoelectric Point (pI)

65.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1342
Acc36I ACCTGC 2 cut(s) 259, 842
AccB7I CCANNNNNTGG 1 cut(s) 1116
AccBSI CCGCTC 2 cut(s) 357, 748
AccI GTMKAC 1 cut(s) 322
AccII CGCG 2 cut(s) 375, 646
AccIII TCCGGA 2 cut(s) 1065, 1168
AciI CCGC 4 cut(s) 159, 357, 746, 892
AclWI GGATC 5 cut(s) 705, 814, 1181, 1292, 1358
AcoI YGGCCR 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 1339
AfaI GTAC 4 cut(s) 194, 918, 1007, 1377
AfiI CCNNNNNNNGG 6 cut(s) 116, 168, 1055, 1116, 1244, 1385
AflIII ACRYGT 1 cut(s) 644
AgsI TTSAA 6 cut(s) 207, 403, 526, 557, 935, 1078
AjnI CCWGG 2 cut(s) 91, 1264
AluBI AGCT 6 cut(s) 211, 352, 953, 1230, 1336, 1418
AluI AGCT 6 cut(s) 211, 352, 953, 1230, 1336, 1418
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 2 cut(s) 221, 448
AlwI GGATC 5 cut(s) 705, 814, 1181, 1292, 1358
Ama87I CYCGRG 1 cut(s) 407
Aor13HI TCCGGA 2 cut(s) 1065, 1168
AoxI GGCC 2 cut(s) 118, 196
ApeKI GCWGC 1 cut(s) 254
ArsI GACNNNNNNTTYG 8 cut(s) 115, 147, 219, 251, 1073, 1105, 1281, 1313
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 2 cut(s) 375, 1343
AspS9I GGNCC 5 cut(s) 197, 248, 439, 679, 1250
AsuC2I CCSGG 2 cut(s) 626, 1282
AsuHPI GGTGA 3 cut(s) 66, 344, 1396
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 4 cut(s) 248, 439, 679, 1250
BbsI GAAGAC 2 cut(s) 125, 229
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 241
BccI CCATC 4 cut(s) 50, 430, 838, 1183
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 2 cut(s) 93, 1266
BcnI CCSGG 2 cut(s) 626, 1282
BcoDI GTCTC 2 cut(s) 221, 448
BfaI CTAG 1 cut(s) 954
BfuAI ACCTGC 2 cut(s) 259, 842
BisI GCNGC 3 cut(s) 255, 358, 893
BlpI GCTNAGC 2 cut(s) 353, 773
BlsI GCNGC 3 cut(s) 256, 359, 894
Bme1390I CCNGG 4 cut(s) 93, 626, 1266, 1282
Bme18I GGWCC 4 cut(s) 248, 439, 679, 1250
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 5 cut(s) 197, 248, 439, 679, 1250
BmiI GGNNCC 3 cut(s) 87, 680, 1173
BmrFI CCNGG 4 cut(s) 93, 626, 1266, 1282
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 2 cut(s) 456, 1220
BmuI ACTGGG 1 cut(s) 194
BpiI GAAGAC 2 cut(s) 125, 229
BplI GAGNNNNNCTC 4 cut(s) 564, 596, 1027, 1059
BpmI CTGGAG 2 cut(s) 75, 368
Bpu1102I GCTNAGC 2 cut(s) 353, 773
BpuEI CTTGAG 4 cut(s) 401, 482, 647, 746
BpuMI CCSGG 2 cut(s) 626, 1282
BsaBI GATNNNNATC 1 cut(s) 1289
BsaJI CCNNGG 2 cut(s) 127, 966
BsaWI WCCGGW 3 cut(s) 336, 1065, 1168
Bsc4I CCNNNNNNNGG 6 cut(s) 116, 168, 1055, 1116, 1244, 1385
Bse1I ACTGG 5 cut(s) 200, 276, 297, 351, 1373
Bse8I GATNNNNATC 1 cut(s) 1289
BseAI TCCGGA 2 cut(s) 1065, 1168
BseBI CCWGG 2 cut(s) 93, 1266
BseDI CCNNGG 2 cut(s) 127, 966
BseGI GGATG 3 cut(s) 36, 1104, 1175
BseJI GATNNNNATC 1 cut(s) 1289
BseLI CCNNNNNNNGG 6 cut(s) 116, 168, 1055, 1116, 1244, 1385
BseMII CTCAG 3 cut(s) 344, 1334, 1397
BseNI ACTGG 5 cut(s) 200, 276, 297, 351, 1373
BseRI GAGGAG 2 cut(s) 1035, 1260
BseXI GCAGC 1 cut(s) 241
BsgI GTGCAG 1 cut(s) 809
Bsh1236I CGCG 2 cut(s) 375, 646
BshFI GGCC 2 cut(s) 120, 198
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 5 cut(s) 337, 626, 1066, 1169, 1282
BslFI GGGAC 2 cut(s) 608, 1401
BslI CCNNNNNNNGG 6 cut(s) 116, 168, 1055, 1116, 1244, 1385
BsmAI GTCTC 2 cut(s) 221, 448
BsmFI GGGAC 2 cut(s) 608, 1401
BsnI GGCC 2 cut(s) 120, 198
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 1 cut(s) 388
Bsp13I TCCGGA 2 cut(s) 1065, 1168
Bsp1407I TGTACA 1 cut(s) 916
Bsp143I GATC 7 cut(s) 71, 364, 697, 819, 1186, 1284, 1363
Bsp1720I GCTNAGC 2 cut(s) 353, 773
BspACI CCGC 4 cut(s) 159, 357, 746, 892
BspANI GGCC 2 cut(s) 120, 198
BspCNI CTCAG 3 cut(s) 345, 1333, 1396
BspEI TCCGGA 2 cut(s) 1065, 1168
BspFNI CGCG 2 cut(s) 375, 646
BspHI TCATGA 1 cut(s) 856
BspLI GGNNCC 3 cut(s) 87, 680, 1173
BspMI ACCTGC 2 cut(s) 259, 842
BspPI GGATC 5 cut(s) 705, 814, 1181, 1292, 1358
BsrBI CCGCTC 2 cut(s) 357, 748
BsrGI TGTACA 1 cut(s) 916
BsrI ACTGG 5 cut(s) 200, 276, 297, 351, 1373
BssECI CCNNGG 2 cut(s) 127, 966
BssMI GATC 7 cut(s) 71, 364, 697, 819, 1186, 1284, 1363
Bst2UI CCWGG 2 cut(s) 93, 1266
Bst4CI ACNGT 3 cut(s) 22, 235, 496
Bst6I CTCTTC 1 cut(s) 966
BstAUI TGTACA 1 cut(s) 916
BstC8I GCNNGC 3 cut(s) 150, 482, 770
BstDEI CTNAG 5 cut(s) 212, 353, 773, 1320, 1383
BstEII GGTNACC 1 cut(s) 332
BstF5I GGATG 3 cut(s) 36, 1104, 1175
BstFNI CGCG 2 cut(s) 375, 646
BstHHI GCGC 2 cut(s) 375, 1343
BstKTI GATC 7 cut(s) 74, 367, 700, 822, 1189, 1287, 1366
BstMAI GTCTC 2 cut(s) 221, 448
BstMBI GATC 7 cut(s) 71, 364, 697, 819, 1186, 1284, 1363
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 481, 643, 774
BstNI CCWGG 2 cut(s) 93, 1266
BstPI GGTNACC 1 cut(s) 332
BstSCI CCNGG 4 cut(s) 91, 624, 1264, 1280
BstUI CGCG 2 cut(s) 375, 646
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 2 cut(s) 125, 229
BstX2I RGATCY 3 cut(s) 697, 819, 1363
BstYI RGATCY 3 cut(s) 697, 819, 1363
BsuRI GGCC 2 cut(s) 120, 198
BtsCI GGATG 3 cut(s) 36, 1104, 1175
BtsIMutI CAGTG 1 cut(s) 283
BveI ACCTGC 2 cut(s) 259, 842
Cac8I GCNNGC 3 cut(s) 150, 482, 770
CciI TCATGA 1 cut(s) 856
CfoI GCGC 2 cut(s) 375, 1343
Cfr13I GGNCC 5 cut(s) 197, 248, 439, 679, 1250
Csp6I GTAC 4 cut(s) 193, 917, 1006, 1376
CviAII CATG 8 cut(s) 145, 245, 476, 857, 981, 1011, 1329, 1369
CviQI GTAC 4 cut(s) 193, 917, 1006, 1376
DdeI CTNAG 5 cut(s) 212, 353, 773, 1320, 1383
DpnI GATC 7 cut(s) 73, 366, 699, 821, 1188, 1286, 1365
DpnII GATC 7 cut(s) 71, 364, 697, 819, 1186, 1284, 1363
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 966
EarI CTCTTC 1 cut(s) 966
Eco47I GGWCC 4 cut(s) 248, 439, 679, 1250
Eco57I CTGAAG 1 cut(s) 1339
Eco88I CYCGRG 1 cut(s) 407
Eco91I GGTNACC 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 1250
EcoO65I GGTNACC 1 cut(s) 332
EcoRII CCWGG 2 cut(s) 91, 1264
EcoT22I ATGCAT 1 cut(s) 471
FaeI CATG 8 cut(s) 148, 248, 479, 860, 984, 1014, 1332, 1372
FalI AAGNNNNNCTT 1 cut(s) 1402
FaqI GGGAC 2 cut(s) 608, 1401
FatI CATG 8 cut(s) 144, 244, 475, 856, 980, 1010, 1328, 1368
FauNDI CATATG 1 cut(s) 1149
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 3 cut(s) 255, 358, 893
FokI GGATG 3 cut(s) 23, 1091, 1162
Fsp4HI GCNGC 3 cut(s) 255, 358, 893
FspBI CTAG 1 cut(s) 954
FspI TGCGCA 1 cut(s) 1342
GlaI GCGC 2 cut(s) 374, 1342
GluI GCNGC 3 cut(s) 255, 358, 893
GsuI CTGGAG 2 cut(s) 75, 368
HaeIII GGCC 2 cut(s) 120, 198
HapII CCGG 5 cut(s) 337, 626, 1066, 1169, 1282
HhaI GCGC 2 cut(s) 375, 1343
Hin1II CATG 8 cut(s) 148, 248, 479, 860, 984, 1014, 1332, 1372
Hin6I GCGC 2 cut(s) 373, 1341
HinP1I GCGC 2 cut(s) 373, 1341
HincII GTYRAC 2 cut(s) 302, 978
HindII GTYRAC 2 cut(s) 302, 978
HindIII AAGCTT 1 cut(s) 1416
HinfI GANTC 4 cut(s) 183, 311, 410, 1144
HpaII CCGG 5 cut(s) 337, 626, 1066, 1169, 1282
HphI GGTGA 3 cut(s) 66, 344, 1396
Hpy166II GTNNAC 6 cut(s) 302, 323, 919, 978, 1008, 1326
Hpy188I TCNGA 4 cut(s) 140, 709, 970, 1191
Hpy188III TCNNGA 6 cut(s) 400, 451, 857, 935, 1066, 1169
Hpy8I GTNNAC 6 cut(s) 302, 323, 919, 978, 1008, 1326
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 3 cut(s) 602, 649, 938
HpyCH4III ACNGT 3 cut(s) 22, 235, 496
HpyCH4V TGCA 4 cut(s) 59, 469, 826, 1073
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 481, 643, 774
HpyF3I CTNAG 5 cut(s) 212, 353, 773, 1320, 1383
Hsp92II CATG 8 cut(s) 148, 248, 479, 860, 984, 1014, 1332, 1372
HspAI GCGC 2 cut(s) 373, 1341
Kpn2I TCCGGA 2 cut(s) 1065, 1168
Kzo9I GATC 7 cut(s) 71, 364, 697, 819, 1186, 1284, 1363
LmnI GCTCC 4 cut(s) 349, 542, 753, 1171
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 2 cut(s) 456, 1220
MaeI CTAG 1 cut(s) 954
MaeIII GTNAC 4 cut(s) 332, 411, 647, 1315
MalI GATC 7 cut(s) 73, 366, 699, 821, 1188, 1286, 1365
MbiI CCGCTC 2 cut(s) 357, 748
MboI GATC 7 cut(s) 71, 364, 697, 819, 1186, 1284, 1363
MboII GAAGA 6 cut(s) 7, 125, 229, 923, 956, 983
MflI RGATCY 3 cut(s) 697, 819, 1363
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 3 cut(s) 632, 735, 1302
MluI ACGCGT 1 cut(s) 644
MlyI GAGTC 3 cut(s) 320, 419, 1138
Mph1103I ATGCAT 1 cut(s) 471
MroI TCCGGA 2 cut(s) 1065, 1168
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 2 cut(s) 630, 1423
MslI CAYNNNNRTG 1 cut(s) 474
MspI CCGG 5 cut(s) 337, 626, 1066, 1169, 1282
MspR9I CCNGG 4 cut(s) 93, 626, 1266, 1282
MvaI CCWGG 2 cut(s) 93, 1266
MvnI CGCG 2 cut(s) 375, 646
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 481, 643, 774
NciI CCSGG 2 cut(s) 626, 1282
NdeI CATATG 1 cut(s) 1149
NdeII GATC 7 cut(s) 71, 364, 697, 819, 1186, 1284, 1363
NlaIII CATG 8 cut(s) 148, 248, 479, 860, 984, 1014, 1332, 1372
NlaIV GGNNCC 3 cut(s) 87, 680, 1173
NmuCI GTSAC 2 cut(s) 332, 411
NsbI TGCGCA 1 cut(s) 1342
NsiI ATGCAT 1 cut(s) 471
PagI TCATGA 1 cut(s) 856
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 1 cut(s) 183
PflMI CCANNNNNTGG 1 cut(s) 1116
PfoI TCCNGGA 1 cut(s) 1264
PkrI GCNGC 3 cut(s) 256, 359, 894
PleI GAGTC 3 cut(s) 319, 418, 1138
PpsI GAGTC 3 cut(s) 319, 418, 1138
PpuMI RGGWCCY 1 cut(s) 1250
Psp5II RGGWCCY 1 cut(s) 1250
Psp6I CCWGG 2 cut(s) 91, 1264
PspEI GGTNACC 1 cut(s) 332
PspGI CCWGG 2 cut(s) 91, 1264
PspN4I GGNNCC 3 cut(s) 87, 680, 1173
PspPI GGNCC 5 cut(s) 197, 248, 439, 679, 1250
PspPPI RGGWCCY 1 cut(s) 1250
PsuI RGATCY 3 cut(s) 697, 819, 1363
RsaI GTAC 4 cut(s) 194, 918, 1007, 1377
RsaNI GTAC 4 cut(s) 193, 917, 1006, 1376
RseI CAYNNNNRTG 1 cut(s) 474
SaqAI TTAA 2 cut(s) 630, 1423
SatI GCNGC 3 cut(s) 255, 358, 893
Sau3AI GATC 7 cut(s) 71, 364, 697, 819, 1186, 1284, 1363
Sau96I GGNCC 5 cut(s) 197, 248, 439, 679, 1250
SchI GAGTC 3 cut(s) 320, 419, 1138
ScrFI CCNGG 4 cut(s) 93, 626, 1266, 1282
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 2 cut(s) 456, 1220
SinI GGWCC 4 cut(s) 248, 439, 679, 1250
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 4 cut(s) 416, 461, 662, 725
SmoI CTYRAG 4 cut(s) 416, 461, 662, 725
Sse9I AATT 3 cut(s) 632, 735, 1302
SsiI CCGC 4 cut(s) 159, 357, 746, 892
SspI AATATT 2 cut(s) 832, 1125
SspMI CTAG 1 cut(s) 954
StyD4I CCNGG 4 cut(s) 91, 624, 1264, 1280
TaaI ACNGT 3 cut(s) 22, 235, 496
TaqI TCGA 5 cut(s) 186, 781, 1086, 1255, 1287
TaqII GACCGA 1 cut(s) 119
TasI AATT 3 cut(s) 632, 735, 1302
TatI WGTACW 2 cut(s) 916, 1005
TauI GCSGC 2 cut(s) 360, 895
TfiI GAWTC 1 cut(s) 183
Tru1I TTAA 2 cut(s) 630, 1423
Tru9I TTAA 2 cut(s) 630, 1423
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 2 cut(s) 332, 411
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 2 cut(s) 332, 411
TspDTI ATGAA 9 cut(s) 17, 229, 233, 256, 524, 591, 855, 1016, 1295
TspRI CASTG 1 cut(s) 283
Van91I CCANNNNNTGG 1 cut(s) 1116
VpaK11BI GGWCC 4 cut(s) 248, 439, 679, 1250
XmiI GTMKAC 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 1 cut(s) 954
Zsp2I ATGCAT 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.