FvH4_7g25900

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000704
Physical Location & Seq
Forward (+)
19708532 .. 19710215
1684 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25900.t1

Sequence Viewer

Length: 1443 bp
ATGGAAGATCTCTCCTTTATGATCCTAGTTTTTACCCTCACAGCACTTTTTGCTGGGTATTTCATCAAGTTTAAAACATCACTAGGTAAGAAACTCCCACCGGGGTCATTTGGTCTTCCTTTAATTGGAGAGTCCATTAGCTTCATCCGAGCACAAAAACAGGACGGATCCGATGAGTGGATCCGGTCTCGTATCCGCAAGTATGGGCCGGTGTTCAAGACCTCTCTCATGGGGTCTAAAACGATCGTTATAACTGGCCAAGCTGGAAACCGGTTTGTGTTTGGTGGAGGTGACACTGGTATTGGAGGCAACCAAGTAGCAACTGTAGCCAGCATTCTTGGAAAACAAAGCATATTCGAGCTTTCTGGATCCAGGCACAAGCTTGTGAGGAGTGCCATAACCAACTTCCTAAAGCCAGAAAACATTCAAAGAATTGTAGGCGAAACCGATTTGTTAATCAAAGACCAAATTTTCAAGGAACTTCAAGGCAAGGATATAGTGCAACTAGTGCTTTTGGTGAAGAGATTAACATTCAAGGTTACTTGCAGTTTGTTTTTTGGACTACCAGAAGGCAAAGACAAAGATGCATTGTTTGAAGACTTTACTATTGCCATCAAAGGCCTGTGGGCAATTCCATTAAACCTTCCAGGCACTCAATTCTACAAAGCAGTACAAGCACGTAGCAGGATAGCCAAACTCCTCACGAAGGTGGTGGAGGAAAAGAAGAACAAGGCAACAAAAGTGACCCCCCAGAACAATGACATAATCTCAGCTCTGCTACTTCTGAGAGATGAAGATGGTGAGCCTCTGCAAGAGCAAGAGATCGTTGATAATCTCATTACCCTGATAGTTGCTAGCCACGACACCACCTCGATTCTCCTGAGCCTCCTGTTAAGGCATCTTTCTACAGATTCCAAGATTTTCAGTGAGGTGCTTCAAGAGCAAGATGAAGTAGCTAAGGCTGTGGAAGGAAGTGAAAGAAAGGTTATTACATGGAATGAAATCCAGAAGATGAAGCACACATGGAGAGTGGCACAAGAGCTCATGAGGATGACTCCCCCTGCTTTTGGAAACTTCAAGTGTGCATGGAGAGACACTACCTTTGATGGCTATCATATCCCCAAGGGATGGAAGGTTTTCTGGGTGGCATCTGGAACACACATGGACAGCAACATATTTGAAGATCCAAACAAGTTTGATCCATCACGATTCGAGAACTCAACGAAATCGTTTCCCCCATACACATACATTGCATTCGGAGCAGGCCCGCGAATTTGTCCCGGGGCAGAATTTGCAAGGATTGAAGCGCTGCTAACGATTCATCATTTGATAACAAAGTATTCATGGACGAAGATGATCAAGGATGAGCCTTTAATACGTGAACCCCTGCCTTATCCAGCCATGGGACTTCCAGTCAAGCTTCACCAAAGGAATGATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

481

Amino Acids

54.02

Weight (kDa)

9.24

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 461 8.3e-65 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 1128
AccII CGCG 1 cut(s) 1270
AciI CCGC 2 cut(s) 196, 1268
AclWI GGATC 9 cut(s) 16, 162, 175, 175, 188, 363, 376, 1178, 1193
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 3 cut(s) 468, 1272, 1289
AfaI GTAC 1 cut(s) 672
AfeI AGCGCT 1 cut(s) 1308
AfiI CCNNNNNNNGG 6 cut(s) 125, 177, 706, 1067, 1128, 1403
AgeI ACCGGT 1 cut(s) 270
AhdI GACNNNNNGTC 1 cut(s) 1412
AhlI ACTAGT 1 cut(s) 505
AjnI CCWGG 2 cut(s) 371, 646
AleI CACNNNNGTG 1 cut(s) 707
AluBI AGCT 8 cut(s) 141, 263, 361, 382, 773, 956, 1042, 1420
AluI AGCT 8 cut(s) 141, 263, 361, 382, 773, 956, 1042, 1420
Alw21I GWGCWC 2 cut(s) 154, 1044
Alw26I GTCTC 2 cut(s) 192, 1086
AlwI GGATC 9 cut(s) 16, 162, 175, 175, 188, 363, 376, 1178, 1193
Ama87I CYCGRG 1 cut(s) 1280
Aor51HI AGCGCT 1 cut(s) 1308
AoxI GGCC 4 cut(s) 206, 256, 619, 1264
ApeKI GCWGC 1 cut(s) 1309
ApoI RAATTY 3 cut(s) 468, 1272, 1289
ArsI GACNNNNNNTTYG 2 cut(s) 1085, 1117
AsiGI ACCGGT 1 cut(s) 270
Asp700I GAANNNNTTC 2 cut(s) 423, 1136
AspLEI GCGC 1 cut(s) 1309
AspS9I GGNCC 2 cut(s) 206, 1265
AsuC2I CCSGG 3 cut(s) 102, 1281, 1282
AsuHPI GGTGA 4 cut(s) 302, 529, 812, 1415
AsuNHI GCTAGC 1 cut(s) 854
AvaI CYCGRG 1 cut(s) 1280
BalI TGGCCA 1 cut(s) 258
BamHI GGATCC 3 cut(s) 167, 180, 368
BanII GRGCYC 1 cut(s) 1044
BbsI GAAGAC 2 cut(s) 107, 603
Bbv12I GWGCWC 2 cut(s) 154, 1044
BbvI GCAGC 1 cut(s) 1296
BccI CCATC 5 cut(s) 620, 791, 1100, 1122, 1210
BciT130I CCWGG 2 cut(s) 373, 648
BciVI GTATCC 1 cut(s) 203
BclI TGATCA 1 cut(s) 1356
BcnI CCSGG 3 cut(s) 102, 1281, 1282
BcoDI GTCTC 2 cut(s) 192, 1086
BcuI ACTAGT 1 cut(s) 505
BfaI CTAG 4 cut(s) 26, 83, 506, 855
BfmI CTRYAG 2 cut(s) 324, 906
BfoI RGCGCY 1 cut(s) 1310
BfuI GTATCC 1 cut(s) 203
BglII AGATCT 1 cut(s) 7
BisI GCNGC 1 cut(s) 1310
BlsI GCNGC 1 cut(s) 1311
Bme1390I CCNGG 5 cut(s) 102, 373, 648, 1281, 1282
BmeRI GACNNNNNGTC 1 cut(s) 1412
BmeT110I CYCGRG 1 cut(s) 1280
BmgT120I GGNCC 2 cut(s) 206, 1265
BmiI GGNNCC 3 cut(s) 169, 182, 370
BmrFI CCNGG 5 cut(s) 102, 373, 648, 1281, 1282
BmsI GCATC 3 cut(s) 574, 907, 1157
BmtI GCTAGC 1 cut(s) 858
BpiI GAAGAC 2 cut(s) 107, 603
BplI GAGNNNNNCTC 2 cut(s) 1039, 1071
Bpu10I CCTNAGC 2 cut(s) 881, 957
BpuMI CCSGG 3 cut(s) 102, 1281, 1282
BsaAI YACGTR 2 cut(s) 680, 1379
BsaBI GATNNNNATC 1 cut(s) 1110
BsaI GGTCTC 1 cut(s) 192
BsaJI CCNNGG 5 cut(s) 101, 1122, 1280, 1281, 1401
BsaWI WCCGGW 2 cut(s) 183, 270
Bsc4I CCNNNNNNNGG 6 cut(s) 125, 177, 706, 1067, 1128, 1403
Bse118I RCCGGY 2 cut(s) 208, 270
Bse1I ACTGG 3 cut(s) 259, 301, 1412
Bse3DI GCAATG 1 cut(s) 1248
Bse8I GATNNNNATC 1 cut(s) 1110
BseBI CCWGG 2 cut(s) 373, 648
BseDI CCNNGG 5 cut(s) 101, 1122, 1280, 1281, 1401
BseGI GGATG 4 cut(s) 144, 1056, 1133, 1369
BseJI GATNNNNATC 1 cut(s) 1110
BseLI CCNNNNNNNGG 6 cut(s) 125, 177, 706, 1067, 1128, 1403
BseMI GCAATG 1 cut(s) 1248
BseMII CTCAG 3 cut(s) 776, 783, 872
BseNI ACTGG 3 cut(s) 259, 301, 1412
BseRI GAGGAG 2 cut(s) 403, 689
BseXI GCAGC 1 cut(s) 1296
BseYI CCCAGC 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 1270
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 4 cut(s) 208, 258, 621, 1266
BshTI ACCGGT 1 cut(s) 270
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 2 cut(s) 154, 1044
BsiHKCI CYCGRG 1 cut(s) 1280
BsiSI CCGG 5 cut(s) 101, 184, 209, 271, 1281
BslFI GGGAC 2 cut(s) 1263, 1419
BslI CCNNNNNNNGG 6 cut(s) 125, 177, 706, 1067, 1128, 1403
BsmAI GTCTC 2 cut(s) 192, 1086
BsmFI GGGAC 2 cut(s) 1263, 1419
BsmI GAATGC 2 cut(s) 333, 1253
BsnI GGCC 4 cut(s) 208, 258, 621, 1266
Bso31I GGTCTC 1 cut(s) 192
BsoBI CYCGRG 1 cut(s) 1280
Bsp1286I GDGCHC 2 cut(s) 154, 1044
Bsp19I CCATGG 1 cut(s) 1401
BspACI CCGC 2 cut(s) 196, 1268
BspANI GGCC 4 cut(s) 208, 258, 621, 1266
BspCNI CTCAG 3 cut(s) 777, 782, 873
BspFNI CGCG 1 cut(s) 1270
BspHI TCATGA 1 cut(s) 1044
BspLI GGNNCC 3 cut(s) 169, 182, 370
BspOI GCTAGC 1 cut(s) 858
BspPI GGATC 9 cut(s) 16, 162, 175, 175, 188, 363, 376, 1178, 1193
BspTNI GGTCTC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 1248
BsrFI RCCGGY 2 cut(s) 208, 270
BsrI ACTGG 3 cut(s) 259, 301, 1412
BssAI RCCGGY 2 cut(s) 208, 270
BssECI CCNNGG 5 cut(s) 101, 1122, 1280, 1281, 1401
BssT1I CCWWGG 2 cut(s) 1122, 1401
Bst2UI CCWGG 2 cut(s) 373, 648
Bst4CI ACNGT 1 cut(s) 325
Bst6I CTCTTC 1 cut(s) 515
BstAPI GCANNNNNTGC 3 cut(s) 50, 508, 1292
BstBAI YACGTR 2 cut(s) 680, 1379
BstC8I GCNNGC 4 cut(s) 331, 856, 1264, 1268
BstDEI CTNAG 4 cut(s) 769, 785, 881, 957
BstDSI CCRYGG 1 cut(s) 1401
BstENI CCTNNNNNAGG 1 cut(s) 704
BstF5I GGATG 4 cut(s) 144, 1056, 1133, 1369
BstFNI CGCG 1 cut(s) 1270
BstH2I RGCGCY 1 cut(s) 1310
BstHHI GCGC 1 cut(s) 1309
BstMAI GTCTC 2 cut(s) 192, 1086
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 7 cut(s) 50, 326, 508, 674, 940, 1259, 1292
BstNI CCWGG 2 cut(s) 373, 648
BstSCI CCNGG 5 cut(s) 100, 371, 646, 1279, 1280
BstSFI CTRYAG 2 cut(s) 324, 906
BstUI CGCG 1 cut(s) 1270
BstV1I GCAGC 1 cut(s) 1296
BstV2I GAAGAC 2 cut(s) 107, 603
BstX2I RGATCY 5 cut(s) 7, 167, 180, 368, 1183
BstYI RGATCY 5 cut(s) 7, 167, 180, 368, 1183
BsuI GTATCC 1 cut(s) 203
BsuRI GGCC 4 cut(s) 208, 258, 621, 1266
BtgI CCRYGG 1 cut(s) 1401
BtsCI GGATG 4 cut(s) 144, 1056, 1133, 1369
BtsIMutI CAGTG 2 cut(s) 294, 931
Cac8I GCNNGC 4 cut(s) 331, 856, 1264, 1268
CciI TCATGA 1 cut(s) 1044
CfoI GCGC 1 cut(s) 1309
Cfr10I RCCGGY 2 cut(s) 208, 270
Cfr13I GGNCC 2 cut(s) 206, 1265
Cfr9I CCCGGG 1 cut(s) 1280
Csp6I GTAC 1 cut(s) 671
CspAI ACCGGT 1 cut(s) 270
CviAII CATG 8 cut(s) 229, 993, 1023, 1045, 1086, 1162, 1344, 1402
CviQI GTAC 1 cut(s) 671
DdeI CTNAG 4 cut(s) 769, 785, 881, 957
DraI TTTAAA 1 cut(s) 73
DriI GACNNNNNGTC 1 cut(s) 1412
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 515
Eam1105I GACNNNNNGTC 1 cut(s) 1412
EarI CTCTTC 1 cut(s) 515
Ecl136II GAGCTC 1 cut(s) 1042
Eco130I CCWWGG 2 cut(s) 1122, 1401
Eco147I AGGCCT 1 cut(s) 621
Eco24I GRGCYC 1 cut(s) 1044
Eco31I GGTCTC 1 cut(s) 192
Eco47III AGCGCT 1 cut(s) 1308
Eco53kI GAGCTC 1 cut(s) 1042
Eco88I CYCGRG 1 cut(s) 1280
EcoICRI GAGCTC 1 cut(s) 1042
EcoNI CCTNNNNNAGG 1 cut(s) 704
EcoRII CCWGG 2 cut(s) 371, 646
EcoT14I CCWWGG 2 cut(s) 1122, 1401
EcoT22I ATGCAT 1 cut(s) 589
EcoT38I GRGCYC 1 cut(s) 1044
ErhI CCWWGG 2 cut(s) 1122, 1401
FaeI CATG 8 cut(s) 232, 996, 1026, 1048, 1089, 1165, 1347, 1405
FaqI GGGAC 2 cut(s) 1263, 1419
FatI CATG 8 cut(s) 228, 992, 1022, 1044, 1085, 1161, 1343, 1401
FauI CCCGC 1 cut(s) 1275
FbaI TGATCA 1 cut(s) 1356
Fnu4HI GCNGC 1 cut(s) 1310
FokI GGATG 4 cut(s) 131, 1063, 1140, 1376
FriOI GRGCYC 1 cut(s) 1044
Fsp4HI GCNGC 1 cut(s) 1310
FspBI CTAG 4 cut(s) 26, 83, 506, 855
GlaI GCGC 1 cut(s) 1308
GluI GCNGC 1 cut(s) 1310
GsaI CCCAGC 1 cut(s) 57
HaeII RGCGCY 1 cut(s) 1310
HaeIII GGCC 4 cut(s) 208, 258, 621, 1266
HapII CCGG 5 cut(s) 101, 184, 209, 271, 1281
HhaI GCGC 1 cut(s) 1309
Hin1II CATG 8 cut(s) 232, 996, 1026, 1048, 1089, 1165, 1347, 1405
Hin6I GCGC 1 cut(s) 1307
HinP1I GCGC 1 cut(s) 1307
HindIII AAGCTT 2 cut(s) 380, 1418
HinfI GANTC 6 cut(s) 131, 874, 911, 1054, 1209, 1318
HpaII CCGG 5 cut(s) 101, 184, 209, 271, 1281
HphI GGTGA 4 cut(s) 302, 529, 812, 1415
Hpy166II GTNNAC 1 cut(s) 1382
Hpy188I TCNGA 4 cut(s) 149, 172, 786, 1259
Hpy8I GTNNAC 1 cut(s) 1382
HpyAV CCTTC 5 cut(s) 563, 653, 700, 962, 1126
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4IV ACGT 2 cut(s) 679, 1378
HpyCH4V TGCA 7 cut(s) 502, 546, 587, 811, 1085, 1253, 1295
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 326, 508, 674, 940, 1259, 1292
HpyF3I CTNAG 4 cut(s) 769, 785, 881, 957
HpySE526I ACGT 2 cut(s) 679, 1378
Hsp92II CATG 8 cut(s) 232, 996, 1026, 1048, 1089, 1165, 1347, 1405
HspAI GCGC 1 cut(s) 1307
Ksp22I TGATCA 1 cut(s) 1356
LmnI GCTCC 1 cut(s) 1259
Lsp1109I GCAGC 1 cut(s) 1296
LweI GCATC 3 cut(s) 574, 907, 1157
MaeI CTAG 4 cut(s) 26, 83, 506, 855
MaeII ACGT 2 cut(s) 679, 1378
MaeIII GTNAC 3 cut(s) 290, 538, 742
MboII GAAGA 9 cut(s) 17, 107, 532, 608, 736, 806, 1021, 1193, 1363
MflI RGATCY 5 cut(s) 7, 167, 180, 368, 1183
MhlI GDGCHC 2 cut(s) 154, 1044
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 7 cut(s) 123, 432, 468, 630, 656, 1272, 1289
MluNI TGGCCA 1 cut(s) 258
MlyI GAGTC 2 cut(s) 140, 1048
Mox20I TGGCCA 1 cut(s) 258
Mph1103I ATGCAT 1 cut(s) 589
MroXI GAANNNNTTC 2 cut(s) 423, 1136
MscI TGGCCA 1 cut(s) 258
MseI TTAA 7 cut(s) 72, 122, 455, 527, 638, 893, 1373
MslI CAYNNNNRTG 2 cut(s) 707, 1049
Msp20I TGGCCA 1 cut(s) 258
MspI CCGG 5 cut(s) 101, 184, 209, 271, 1281
MspR9I CCNGG 5 cut(s) 102, 373, 648, 1281, 1282
Mva1269I GAATGC 2 cut(s) 333, 1253
MvaI CCWGG 2 cut(s) 373, 648
MvnI CGCG 1 cut(s) 1270
MwoI GCNNNNNNNGC 7 cut(s) 50, 326, 508, 674, 940, 1259, 1292
NciI CCSGG 3 cut(s) 102, 1281, 1282
NcoI CCATGG 1 cut(s) 1401
NheI GCTAGC 1 cut(s) 854
NlaIII CATG 8 cut(s) 232, 996, 1026, 1048, 1089, 1165, 1347, 1405
NlaIV GGNNCC 3 cut(s) 169, 182, 370
NmuCI GTSAC 2 cut(s) 290, 742
NsiI ATGCAT 1 cut(s) 589
OliI CACNNNNGTG 1 cut(s) 707
PagI TCATGA 1 cut(s) 1044
PceI AGGCCT 1 cut(s) 621
PctI GAATGC 2 cut(s) 333, 1253
PdmI GAANNNNTTC 2 cut(s) 423, 1136
PfeI GAWTC 4 cut(s) 874, 911, 1209, 1318
PflMI CCANNNNNTGG 1 cut(s) 1128
PinAI ACCGGT 1 cut(s) 270
PkrI GCNGC 1 cut(s) 1311
Ple19I CGATCG 1 cut(s) 246
PleI GAGTC 2 cut(s) 139, 1048
PpsI GAGTC 2 cut(s) 139, 1048
Ppu21I YACGTR 2 cut(s) 680, 1379
PsiI TTATAA 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 1044
Psp6I CCWGG 2 cut(s) 371, 646
PspFI CCCAGC 1 cut(s) 53
PspGI CCWGG 2 cut(s) 371, 646
PspN4I GGNNCC 3 cut(s) 169, 182, 370
PspPI GGNCC 2 cut(s) 206, 1265
PsuI RGATCY 5 cut(s) 7, 167, 180, 368, 1183
PvuI CGATCG 1 cut(s) 246
RsaI GTAC 1 cut(s) 672
RsaNI GTAC 1 cut(s) 671
RseI CAYNNNNRTG 2 cut(s) 707, 1049
SacI GAGCTC 1 cut(s) 1044
SaqAI TTAA 7 cut(s) 72, 122, 455, 527, 638, 893, 1373
SatI GCNGC 1 cut(s) 1310
Sau96I GGNCC 2 cut(s) 206, 1265
SchI GAGTC 2 cut(s) 140, 1048
ScrFI CCNGG 5 cut(s) 102, 373, 648, 1281, 1282
SduI GDGCHC 2 cut(s) 154, 1044
SfaNI GCATC 3 cut(s) 574, 907, 1157
SfcI CTRYAG 2 cut(s) 324, 906
SmaI CCCGGG 1 cut(s) 1282
SmiMI CAYNNNNRTG 2 cut(s) 707, 1049
SpeI ACTAGT 1 cut(s) 505
Sse9I AATT 7 cut(s) 123, 432, 468, 630, 656, 1272, 1289
SseBI AGGCCT 1 cut(s) 621
SsiI CCGC 2 cut(s) 196, 1268
SspMI CTAG 4 cut(s) 26, 83, 506, 855
SstI GAGCTC 1 cut(s) 1044
StuI AGGCCT 1 cut(s) 621
StyD4I CCNGG 5 cut(s) 100, 371, 646, 1279, 1280
StyI CCWWGG 2 cut(s) 1122, 1401
TaaI ACNGT 1 cut(s) 325
TaiI ACGT 2 cut(s) 682, 1381
TaqI TCGA 3 cut(s) 357, 872, 1212
TasI AATT 7 cut(s) 123, 432, 468, 630, 656, 1272, 1289
TatI WGTACW 1 cut(s) 670
TfiI GAWTC 4 cut(s) 874, 911, 1209, 1318
Tru1I TTAA 7 cut(s) 72, 122, 455, 527, 638, 893, 1373
Tru9I TTAA 7 cut(s) 72, 122, 455, 527, 638, 893, 1373
TscAI CASTG 2 cut(s) 301, 931
TseFI GTSAC 2 cut(s) 290, 742
TseI GCWGC 1 cut(s) 1309
Tsp45I GTSAC 2 cut(s) 290, 742
TspDTI ATGAA 8 cut(s) 52, 133, 807, 963, 1014, 1028, 1310, 1332
TspGWI ACGGA 1 cut(s) 180
TspMI CCCGGG 1 cut(s) 1280
TspRI CASTG 2 cut(s) 301, 931
Van91I CCANNNNNTGG 1 cut(s) 1128
XagI CCTNNNNNAGG 1 cut(s) 704
XapI RAATTY 3 cut(s) 468, 1272, 1289
XmaI CCCGGG 1 cut(s) 1280
XmnI GAANNNNTTC 2 cut(s) 423, 1136
XspI CTAG 4 cut(s) 26, 83, 506, 855
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.