MD03G1051800.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000704
Physical Location & Seq
Reverse (-)
4136499 .. 4139052
2554 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1051800.v1.1.491

Sequence Viewer

Length: 1542 bp
ATGTCTTACAATTTTAAATTAAATGTCTTCCATTTTAATTTGAGCATAAATATCTTATTACTTTTCGTCAGTCTGCATAACATCGTACAAAGTTACTACAAACATATGGCTTTCATTTTAGTAATTGCGATCACTTTGATAATAGCTTTCTTCGTTTCAAAATTACTTCTGTTTAAAGGTCAGACCAAAAATCTCCCAGGAGGGTCTCTAGGGTACCCTTTGCTAGGAGAGTCCCTGAGCTTCCGCCGAGCACAGAAGCAAAACCGAGGCCCTCAGTGGTTCGAAGAAAGAGTTTCAAAACATGGCCCAGTTTTCAAAACCTCCTTGATGGGTTCACCAACTGTCGTTGTTACTGGTCAAGCAGGTAACAAGTTTGTTCTTGGTGGTAGAGATGATGCTCTTGCCACCAAGCAACCGCTCGCCCTTGCAAGCATTGCTGGCAAGGAAAATATATTTGAACTTACAGGATCAAGGTACAAAATGGTAAAGGGAGCAATGATGAGCATCTTAAAGCCTGAAATTCTTCAGAAAAATATCAAACCCATGGATGAATTAATCAAGAACATATTACTAAGAGAAACAAAGAACAAAGACACCATAAAAGGAGTGGCCACCATGAAGAAGCTCGCATTTGAATTAGCATCCAACATCCTTACCGGCATCAAGGATGAGCACACGACTGAGGCTTTGTCTGATGATTTATCCATAGCCTTCAAAGCAATTGCGACGATTCCCTTCAATTTTCCAGGCACAGTTTTCTGGAAAGGTCTAAGAGCCAGATCAAGGATTGTAAGTCGTATTTTGCCAATACTTGAGCAAAAAAGGGAGGAGCTTTCAAAGGGGAAGTTGAGCCCTACAAGCGATCTGTTCACTTGCTTACTAACCCTAAGAGATGAAAATCAAAAACCACTTTCTGATGATATGATTATAGATAACTATGTCACCTTAATCATAGCTAGTCATGACACTTCAGCCATCCTTCTGAGCCTAATGATATGGAAGCTATCCAGAGACTCTGAAATCTACAACAAAGTTTTAGAGGAACAAATGGACATTTTAAGGAAGAGGGATGAAGAAGCAGAAGATGGACTAACATGGTCTGAGATGCACAATATGAAATACACATGGAGAGTTGCACAAGAGTTGATGAGGATGGTACCTCCTGTGTTTGGTAGCTTTAGGACAACAGTTAAAGACACTCAATATGGTGGCTACGACATTCCCAAAGGATGGCTGGTGCTTTGGGTGGCACATGGAACTCACATGAACAAAGATGTATTTGATAATCCCACAGAGTTTGATCCATCACGGTTTGAGAACCCATCGAAACCAATCCCTCCATTTACGTATCTTCCATTTGGCGGAGGTCTCCACACTTGCATAGGGAATGAGTTTGTCAAAATAGAATTACTCATTGTCATCCACAACTTGGTGACAAAGTTTGAGTGGTCACAGCTGCACCCTAAGGAAGCAATAACTCGTAACCCTGTGCCATATCCATCCATGGGTCTCCCAATCAAGATCAAACCAAGAAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

514

Amino Acids

58.2

Weight (kDa)

9.42

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 66 - 490 2.5e-57 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 353
Acc65I GGTACC 2 cut(s) 213, 1156
AccB1I GGYRCC 2 cut(s) 213, 1156
AccB7I CCANNNNNTGG 2 cut(s) 1230, 1429
AccBSI CCGCTC 1 cut(s) 418
AciI CCGC 3 cut(s) 244, 416, 1362
AclWI GGATC 2 cut(s) 475, 1295
AcoI YGGCCR 1 cut(s) 609
AcsI RAATTY 1 cut(s) 519
AcuI CTGAAG 2 cut(s) 509, 954
AfaI GTAC 4 cut(s) 87, 215, 476, 1158
AfiI CCNNNNNNNGG 7 cut(s) 224, 783, 1169, 1230, 1361, 1429, 1505
AgsI TTSAA 8 cut(s) 159, 297, 316, 458, 635, 715, 739, 837
AjnI CCWGG 2 cut(s) 196, 745
AluBI AGCT 9 cut(s) 146, 240, 625, 832, 956, 1003, 1176, 1456, 1537
AluI AGCT 9 cut(s) 146, 240, 625, 832, 956, 1003, 1176, 1456, 1537
Alw21I GWGCWC 2 cut(s) 253, 675
Alw26I GTCTC 4 cut(s) 210, 1005, 1373, 1514
AlwI GGATC 2 cut(s) 475, 1295
AoxI GGCC 3 cut(s) 268, 304, 609
ApeKI GCWGC 1 cut(s) 1456
ApoI RAATTY 1 cut(s) 519
ArsI GACNNNNNNTTYG 2 cut(s) 670, 702
AseI ATTAAT 1 cut(s) 554
Asp700I GAANNNNTTC 1 cut(s) 522
Asp718I GGTACC 2 cut(s) 213, 1156
AspS9I GGNCC 2 cut(s) 269, 305
AsuHPI GGTGA 3 cut(s) 327, 934, 1444
AsuII TTCGAA 1 cut(s) 282
AxyI CCTNAGG 1 cut(s) 1464
BalI TGGCCA 1 cut(s) 611
BanI GGYRCC 2 cut(s) 213, 1156
BanII GRGCYC 1 cut(s) 854
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 2 cut(s) 253, 675
BbvI GCAGC 1 cut(s) 1443
BccI CCATC 8 cut(s) 322, 983, 1079, 1147, 1224, 1312, 1330, 1507
BciT130I CCWGG 2 cut(s) 198, 747
BcoDI GTCTC 4 cut(s) 210, 1005, 1373, 1514
BfaI CTAG 3 cut(s) 209, 224, 957
BfuAI ACCTGC 1 cut(s) 353
BisI GCNGC 1 cut(s) 1457
BlsI GCNGC 1 cut(s) 1458
Bme1390I CCNGG 2 cut(s) 198, 747
BmgT120I GGNCC 2 cut(s) 269, 305
BmiI GGNNCC 2 cut(s) 215, 1158
BmrFI CCNGG 2 cut(s) 198, 747
BmrI ACTGGG 1 cut(s) 302
BmsI GCATC 5 cut(s) 385, 513, 650, 669, 1095
BmuI ACTGGG 1 cut(s) 302
BpiI GAAGAC 1 cut(s) 19
Bpu10I CCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 833
BsaAI YACGTR 1 cut(s) 1347
BsaBI GATNNNNATC 2 cut(s) 503, 897
BsaI GGTCTC 3 cut(s) 210, 1373, 1514
BsaJI CCNNGG 4 cut(s) 196, 265, 543, 1503
Bsc4I CCNNNNNNNGG 7 cut(s) 224, 783, 1169, 1230, 1361, 1429, 1505
Bse118I RCCGGY 1 cut(s) 656
Bse1I ACTGG 2 cut(s) 308, 358
Bse21I CCTNAGG 1 cut(s) 1464
Bse3DI GCAATG 2 cut(s) 432, 501
Bse8I GATNNNNATC 2 cut(s) 503, 897
BseBI CCWGG 2 cut(s) 198, 747
BseDI CCNNGG 4 cut(s) 196, 265, 543, 1503
BseJI GATNNNNATC 2 cut(s) 503, 897
BseLI CCNNNNNNNGG 7 cut(s) 224, 783, 1169, 1230, 1361, 1429, 1505
BseMI GCAATG 2 cut(s) 432, 501
BseMII CTCAG 5 cut(s) 227, 287, 672, 974, 1092
BseNI ACTGG 2 cut(s) 308, 358
BseRI GAGGAG 1 cut(s) 842
BseXI GCAGC 1 cut(s) 1443
BsgI GTGCAG 1 cut(s) 1442
BshFI GGCC 3 cut(s) 270, 306, 611
BshNI GGYRCC 2 cut(s) 213, 1156
BsiHKAI GWGCWC 2 cut(s) 253, 675
BsiSI CCGG 1 cut(s) 657
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 7 cut(s) 224, 783, 1169, 1230, 1361, 1429, 1505
BsmAI GTCTC 4 cut(s) 210, 1005, 1373, 1514
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 3 cut(s) 270, 306, 611
Bso31I GGTCTC 3 cut(s) 210, 1373, 1514
Bsp119I TTCGAA 1 cut(s) 282
Bsp1286I GDGCHC 3 cut(s) 253, 675, 854
Bsp143I GATC 6 cut(s) 129, 467, 779, 862, 1300, 1521
Bsp19I CCATGG 2 cut(s) 543, 1503
BspACI CCGC 3 cut(s) 244, 416, 1362
BspANI GGCC 3 cut(s) 270, 306, 611
BspCNI CTCAG 5 cut(s) 228, 286, 673, 975, 1093
BspHI TCATGA 1 cut(s) 961
BspLI GGNNCC 2 cut(s) 215, 1158
BspMI ACCTGC 1 cut(s) 353
BspPI GGATC 2 cut(s) 475, 1295
BspT104I TTCGAA 1 cut(s) 282
BspT107I GGYRCC 2 cut(s) 213, 1156
BspTNI GGTCTC 3 cut(s) 210, 1373, 1514
BsrBI CCGCTC 1 cut(s) 418
BsrDI GCAATG 2 cut(s) 432, 501
BsrFI RCCGGY 1 cut(s) 656
BsrI ACTGG 2 cut(s) 308, 358
BssAI RCCGGY 1 cut(s) 656
BssECI CCNNGG 4 cut(s) 196, 265, 543, 1503
BssMI GATC 6 cut(s) 129, 467, 779, 862, 1300, 1521
BssT1I CCWWGG 2 cut(s) 543, 1503
Bst2UI CCWGG 2 cut(s) 198, 747
Bst4CI ACNGT 4 cut(s) 343, 754, 1189, 1311
Bst6I CTCTTC 1 cut(s) 1058
BstAPI GCANNNNNTGC 1 cut(s) 434
BstBAI YACGTR 1 cut(s) 1347
BstBI TTCGAA 1 cut(s) 282
BstC8I GCNNGC 4 cut(s) 420, 430, 439, 627
BstDEI CTNAG 9 cut(s) 236, 273, 572, 681, 770, 887, 983, 1101, 1464
BstDSI CCRYGG 2 cut(s) 543, 1503
BstENI CCTNNNNNAGG 1 cut(s) 222
BstKTI GATC 6 cut(s) 132, 470, 782, 865, 1303, 1524
BstMAI GTCTC 4 cut(s) 210, 1005, 1373, 1514
BstMBI GATC 6 cut(s) 129, 467, 779, 862, 1300, 1521
BstMWI GCNNNNNNNGC 4 cut(s) 434, 438, 716, 858
BstNI CCWGG 2 cut(s) 198, 747
BstSCI CCNGG 2 cut(s) 196, 745
BstSNI TACGTA 1 cut(s) 1347
BstV1I GCAGC 1 cut(s) 1443
BstV2I GAAGAC 1 cut(s) 19
Bsu36I CCTNAGG 1 cut(s) 1464
BsuRI GGCC 3 cut(s) 270, 306, 611
BtgI CCRYGG 2 cut(s) 543, 1503
BtsIMutI CAGTG 1 cut(s) 281
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 4 cut(s) 420, 430, 439, 627
CciI TCATGA 1 cut(s) 961
Cfr10I RCCGGY 1 cut(s) 656
Cfr13I GGNCC 2 cut(s) 269, 305
Csp6I GTAC 4 cut(s) 86, 214, 475, 1157
CspCI CAANNNNNGTGG 2 cut(s) 1279, 1314
CviAII CATG 9 cut(s) 302, 544, 616, 962, 1095, 1125, 1253, 1264, 1504
CviQI GTAC 4 cut(s) 86, 214, 475, 1157
DdeI CTNAG 9 cut(s) 236, 273, 572, 681, 770, 887, 983, 1101, 1464
DpnI GATC 6 cut(s) 131, 469, 781, 864, 1302, 1523
DpnII GATC 6 cut(s) 129, 467, 779, 862, 1300, 1521
DraI TTTAAA 2 cut(s) 16, 175
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 1 cut(s) 1058
EarI CTCTTC 1 cut(s) 1058
EciI GGCGGA 2 cut(s) 233, 1377
Eco105I TACGTA 1 cut(s) 1347
Eco130I CCWWGG 2 cut(s) 543, 1503
Eco24I GRGCYC 1 cut(s) 854
Eco31I GGTCTC 3 cut(s) 210, 1373, 1514
Eco57I CTGAAG 2 cut(s) 509, 954
Eco81I CCTNAGG 1 cut(s) 1464
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 269
EcoRII CCWGG 2 cut(s) 196, 745
EcoT14I CCWWGG 2 cut(s) 543, 1503
EcoT38I GRGCYC 1 cut(s) 854
ErhI CCWWGG 2 cut(s) 543, 1503
FaeI CATG 9 cut(s) 305, 547, 619, 965, 1098, 1128, 1256, 1267, 1507
FaqI GGGAC 1 cut(s) 217
FatI CATG 9 cut(s) 301, 543, 615, 961, 1094, 1124, 1252, 1263, 1503
FauNDI CATATG 1 cut(s) 105
Fnu4HI GCNGC 1 cut(s) 1457
FriOI GRGCYC 1 cut(s) 854
Fsp4HI GCNGC 1 cut(s) 1457
FspBI CTAG 3 cut(s) 209, 224, 957
GluI GCNGC 1 cut(s) 1457
HaeIII GGCC 3 cut(s) 270, 306, 611
HapII CCGG 1 cut(s) 657
Hin1II CATG 9 cut(s) 305, 547, 619, 965, 1098, 1128, 1256, 1267, 1507
HindIII AAGCTT 1 cut(s) 1535
HinfI GANTC 3 cut(s) 230, 730, 1013
HpaII CCGG 1 cut(s) 657
HphI GGTGA 3 cut(s) 327, 934, 1444
Hpy166II GTNNAC 2 cut(s) 335, 870
Hpy188I TCNGA 7 cut(s) 183, 528, 694, 916, 984, 1018, 1102
Hpy188III TCNNGA 5 cut(s) 559, 760, 962, 1008, 1519
Hpy8I GTNNAC 2 cut(s) 335, 870
Hpy99I CGWCG 1 cut(s) 730
HpyAV CCTTC 3 cut(s) 721, 745, 989
HpyCH4III ACNGT 4 cut(s) 343, 754, 1189, 1311
HpyCH4IV ACGT 1 cut(s) 1346
HpyCH4V TGCA 6 cut(s) 76, 428, 1108, 1136, 1380, 1459
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 438, 716, 858
HpyF3I CTNAG 9 cut(s) 236, 273, 572, 681, 770, 887, 983, 1101, 1464
HpySE526I ACGT 1 cut(s) 1346
Hsp92II CATG 9 cut(s) 305, 547, 619, 965, 1098, 1128, 1256, 1267, 1507
KpnI GGTACC 2 cut(s) 217, 1160
Kzo9I GATC 6 cut(s) 129, 467, 779, 862, 1300, 1521
LmnI GCTCC 2 cut(s) 491, 829
Lsp1109I GCAGC 1 cut(s) 1443
LweI GCATC 5 cut(s) 385, 513, 650, 669, 1095
MaeI CTAG 3 cut(s) 209, 224, 957
MaeII ACGT 1 cut(s) 1346
MaeIII GTNAC 7 cut(s) 92, 349, 365, 940, 1432, 1449, 1481
MalI GATC 6 cut(s) 131, 469, 781, 864, 1302, 1523
MbiI CCGCTC 1 cut(s) 418
MboI GATC 6 cut(s) 129, 467, 779, 862, 1300, 1521
MboII GAAGA 9 cut(s) 19, 142, 296, 515, 631, 1075, 1085, 1094, 1343
MfeI CAATTG 1 cut(s) 720
MhlI GDGCHC 3 cut(s) 253, 675, 854
MlsI TGGCCA 1 cut(s) 611
MluNI TGGCCA 1 cut(s) 611
MlyI GAGTC 2 cut(s) 239, 1007
MmeI TCCRAC 1 cut(s) 669
Mox20I TGGCCA 1 cut(s) 611
MroXI GAANNNNTTC 1 cut(s) 522
MscI TGGCCA 1 cut(s) 611
MseI TTAA 9 cut(s) 15, 20, 36, 174, 509, 554, 947, 1058, 1191
Msp20I TGGCCA 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 1456
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 2 cut(s) 198, 747
MunI CAATTG 1 cut(s) 720
MvaI CCWGG 2 cut(s) 198, 747
MwoI GCNNNNNNNGC 4 cut(s) 434, 438, 716, 858
NcoI CCATGG 2 cut(s) 543, 1503
NdeI CATATG 1 cut(s) 105
NdeII GATC 6 cut(s) 129, 467, 779, 862, 1300, 1521
NlaIII CATG 9 cut(s) 305, 547, 619, 965, 1098, 1128, 1256, 1267, 1507
NlaIV GGNNCC 2 cut(s) 215, 1158
NmeAIII GCCGAG 1 cut(s) 272
NmuCI GTSAC 3 cut(s) 940, 1432, 1449
NspV TTCGAA 1 cut(s) 282
PagI TCATGA 1 cut(s) 961
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 1 cut(s) 730
PflMI CCANNNNNTGG 2 cut(s) 1230, 1429
PkrI GCNGC 1 cut(s) 1458
PleI GAGTC 2 cut(s) 238, 1007
PpsI GAGTC 2 cut(s) 238, 1007
Ppu21I YACGTR 1 cut(s) 1347
PshBI ATTAAT 1 cut(s) 554
Psp6I CCWGG 2 cut(s) 196, 745
PspGI CCWGG 2 cut(s) 196, 745
PspN4I GGNNCC 2 cut(s) 215, 1158
PspPI GGNCC 2 cut(s) 269, 305
PvuII CAGCTG 1 cut(s) 1456
RsaI GTAC 4 cut(s) 87, 215, 476, 1158
RsaNI GTAC 4 cut(s) 86, 214, 475, 1157
SaqAI TTAA 9 cut(s) 15, 20, 36, 174, 509, 554, 947, 1058, 1191
SatI GCNGC 1 cut(s) 1457
Sau3AI GATC 6 cut(s) 129, 467, 779, 862, 1300, 1521
Sau96I GGNCC 2 cut(s) 269, 305
SchI GAGTC 2 cut(s) 239, 1007
ScrFI CCNGG 2 cut(s) 198, 747
SduI GDGCHC 3 cut(s) 253, 675, 854
SfaNI GCATC 5 cut(s) 385, 513, 650, 669, 1095
SfuI TTCGAA 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
SnaBI TACGTA 1 cut(s) 1347
SsiI CCGC 3 cut(s) 244, 416, 1362
SspMI CTAG 3 cut(s) 209, 224, 957
StyD4I CCNGG 2 cut(s) 196, 745
StyI CCWWGG 2 cut(s) 543, 1503
TaaI ACNGT 4 cut(s) 343, 754, 1189, 1311
TaiI ACGT 1 cut(s) 1349
TaqI TCGA 2 cut(s) 282, 1325
TfiI GAWTC 1 cut(s) 730
Tru1I TTAA 9 cut(s) 15, 20, 36, 174, 509, 554, 947, 1058, 1191
Tru9I TTAA 9 cut(s) 15, 20, 36, 174, 509, 554, 947, 1058, 1191
TscAI CASTG 1 cut(s) 281
TseFI GTSAC 3 cut(s) 940, 1432, 1449
TseI GCWGC 1 cut(s) 1456
Tsp45I GTSAC 3 cut(s) 940, 1432, 1449
TspDTI ATGAA 7 cut(s) 103, 564, 632, 909, 1086, 1130, 1280
TspRI CASTG 1 cut(s) 281
Van91I CCANNNNNTGG 2 cut(s) 1230, 1429
VspI ATTAAT 1 cut(s) 554
XagI CCTNNNNNAGG 1 cut(s) 222
XapI RAATTY 1 cut(s) 519
XcmI CCANNNNNNNNNTGG 2 cut(s) 604, 1231
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 3 cut(s) 209, 224, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.