MD03G1205100.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000704
Physical Location & Seq
Forward (+)
28126631 .. 28129094
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1205100.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGAATACCCTTTTCCTCACAGTTTTCCTTTTCCTCATCCCCATCTTGCTTCTCTTTGCAAGGAGAAAAAGATCACCAAAAAGGGTTCCTCCAGGCTCATTAGGATTACCCATAATCGGCCAAAGCCTCGGTCTTCTCCGAGCCATGCGTGCCAACACCGCCGAAAAATGGCTTCAACAAAGAATCGAAAAGTACGGCCCAGTTTCAAAGCTTAGTCTCTTTGGCAAACCAACGGTCTTCATTCATGGACCTGCTGCAAACAAGTTCATATTCGCCAGTGATGACAGCACCATTTCCAGTCAACAAGTTGAGTCTATCCGTCTACTTTTGGGTGACCGGAATATACTGGAGCTGAGCGGCGACGATCACAAGCGCGTCAGAAGTGCTCTTATGTTGTTCTTGAAGCCCGAGTCACTCAAGCAGTATGTGGGAAAGATGGACCAAGAAATCAGGAGACACCTTGAGATGCATTGGCATGGCAAGCAACAAATAACTGTCTTGCCCCTGATGAAAAACCTCACATTCAACATAATATGCTCCCTACTTTTTAGGCTTGAACGGGGAACTCGCAGAGATGAACTCTTGGAGTGTTTCCAAAAAATGATAGAAGGAATGTGGTCAGTCCCGGTTAAATTGCCCTTCACGCGTTACAGCCAGAGCCTCAAGGGAAGCAAGAGGGTCCAAGACATAATCAAGGAACTCATATCGGAAAAGAGGGTAGAACTTGAGCGAAAAACTGCTTCTCCCCTCCAAGACCTTATCACTTGCTTGCTTAGCAGTCGAAGTGATGACAATGGAGAGGTTATGACTGAAAAGGAGATCCTGCACAATGTTATGTTAGTGATGGTCGCAGGTCATGACACTTCATCTATTGTGCTTACTTTTCTATTGCGGCTTCTCGCTAATGACCCTGTTGTACACGCAACCGTTCTTCAAGAGCAAGAAGAAATAGCTAGAAGCAAATCCTCGGAAGAGTTGACATGGAAAGACCTTGCCAAGATGAAGTACACATGGAGAGTTGTTATGGAAACGATGAGAATAACTCCTCCTATCTTTGGTGGCTTCCGGAGTGCATTGAAAGACATCGAGTTTGATGGATTTCTCATCCCCAAAGGGTGGCAAATATTCTGGGCTATGCCTATGACTCATATGGACGATAACATATTTCCGGAGCCATCCAAGTTTGATCCGAATAGGTTTGAGAACCAAGCATCAACTCCACCTTACAGCTTTGTTCCATTTGGAGGAGGACCTCGAAAATGTCCGGGATATGAGTTCGCCCGGATCGAAATCCTTGTTGCAATCCATTATATTGTAACTCAGTTCACATGGAAGCTATGTGCAGACAACAACTTCAGTAGAGATCCGATGCCAGTACCCACTCAAGGACTACCCATAAAAATCATCCCAAGAAAGCTTGTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

476

Amino Acids

54.52

Weight (kDa)

9.49

Isoelectric Point (pI)

61.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 30 - 452 2.5e-78 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 259, 842
AccB7I CCANNNNNTGG 1 cut(s) 1116
AccBSI CCGCTC 1 cut(s) 357
AccI GTMKAC 1 cut(s) 322
AccII CGCG 2 cut(s) 375, 646
AccIII TCCGGA 2 cut(s) 1065, 1168
AciI CCGC 3 cut(s) 159, 357, 892
AclWI GGATC 4 cut(s) 814, 1181, 1292, 1358
AcoI YGGCCR 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 1339
AfaI GTAC 4 cut(s) 194, 918, 1007, 1377
AfiI CCNNNNNNNGG 6 cut(s) 116, 168, 1055, 1116, 1244, 1385
AflIII ACRYGT 1 cut(s) 644
AgsI TTSAA 7 cut(s) 176, 207, 403, 526, 557, 935, 1078
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 6 cut(s) 211, 352, 953, 1230, 1336, 1417
AluI AGCT 6 cut(s) 211, 352, 953, 1230, 1336, 1417
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 2 cut(s) 221, 448
AlwI GGATC 4 cut(s) 814, 1181, 1292, 1358
Ama87I CYCGRG 1 cut(s) 407
Aor13HI TCCGGA 2 cut(s) 1065, 1168
AoxI GGCC 2 cut(s) 118, 196
ApeKI GCWGC 1 cut(s) 254
ArsI GACNNNNNNTTYG 6 cut(s) 115, 147, 219, 251, 1073, 1105
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 5 cut(s) 197, 248, 439, 679, 1250
AsuC2I CCSGG 3 cut(s) 626, 1266, 1282
AsuHPI GGTGA 2 cut(s) 66, 344
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 4 cut(s) 248, 439, 679, 1250
BbsI GAAGAC 2 cut(s) 125, 229
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 241
BccI CCATC 5 cut(s) 50, 430, 838, 1088, 1183
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 1 cut(s) 93
BcnI CCSGG 3 cut(s) 626, 1266, 1282
BcoDI GTCTC 2 cut(s) 221, 448
BfaI CTAG 1 cut(s) 954
BfuAI ACCTGC 2 cut(s) 259, 842
BisI GCNGC 3 cut(s) 255, 358, 893
BlpI GCTNAGC 2 cut(s) 353, 773
BlsI GCNGC 3 cut(s) 256, 359, 894
Bme1390I CCNGG 4 cut(s) 93, 626, 1266, 1282
Bme18I GGWCC 4 cut(s) 248, 439, 679, 1250
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 5 cut(s) 197, 248, 439, 679, 1250
BmiI GGNNCC 3 cut(s) 87, 680, 1173
BmrFI CCNGG 4 cut(s) 93, 626, 1266, 1282
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 3 cut(s) 456, 1220, 1359
BmuI ACTGGG 1 cut(s) 194
BpiI GAAGAC 2 cut(s) 125, 229
BplI GAGNNNNNCTC 4 cut(s) 564, 596, 1027, 1059
BpmI CTGGAG 2 cut(s) 75, 368
Bpu1102I GCTNAGC 2 cut(s) 353, 773
BpuEI CTTGAG 5 cut(s) 401, 482, 647, 746, 1368
BpuMI CCSGG 3 cut(s) 626, 1266, 1282
BsaBI GATNNNNATC 1 cut(s) 1289
BsaJI CCNNGG 2 cut(s) 127, 966
BsaWI WCCGGW 3 cut(s) 336, 1065, 1168
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
Bsc4I CCNNNNNNNGG 6 cut(s) 116, 168, 1055, 1116, 1244, 1385
Bse1I ACTGG 5 cut(s) 200, 276, 297, 351, 1373
Bse8I GATNNNNATC 1 cut(s) 1289
BseAI TCCGGA 2 cut(s) 1065, 1168
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 2 cut(s) 127, 966
BseGI GGATG 4 cut(s) 36, 1104, 1175, 1404
BseJI GATNNNNATC 1 cut(s) 1289
BseLI CCNNNNNNNGG 6 cut(s) 116, 168, 1055, 1116, 1244, 1385
BseMII CTCAG 2 cut(s) 344, 1334
BseNI ACTGG 5 cut(s) 200, 276, 297, 351, 1373
BseRI GAGGAG 2 cut(s) 1035, 1260
BseXI GCAGC 1 cut(s) 241
BsgI GTGCAG 2 cut(s) 809, 1362
Bsh1236I CGCG 2 cut(s) 375, 646
BshFI GGCC 2 cut(s) 120, 198
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 6 cut(s) 337, 626, 1066, 1169, 1265, 1282
BslFI GGGAC 1 cut(s) 608
BslI CCNNNNNNNGG 6 cut(s) 116, 168, 1055, 1116, 1244, 1385
BsmAI GTCTC 2 cut(s) 221, 448
BsmFI GGGAC 1 cut(s) 608
BsnI GGCC 2 cut(s) 120, 198
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 1 cut(s) 388
Bsp13I TCCGGA 2 cut(s) 1065, 1168
Bsp1407I TGTACA 1 cut(s) 916
Bsp143I GATC 6 cut(s) 71, 364, 819, 1186, 1284, 1363
Bsp1720I GCTNAGC 2 cut(s) 353, 773
BspACI CCGC 3 cut(s) 159, 357, 892
BspANI GGCC 2 cut(s) 120, 198
BspCNI CTCAG 2 cut(s) 345, 1333
BspEI TCCGGA 2 cut(s) 1065, 1168
BspFNI CGCG 2 cut(s) 375, 646
BspHI TCATGA 1 cut(s) 856
BspLI GGNNCC 3 cut(s) 87, 680, 1173
BspMI ACCTGC 2 cut(s) 259, 842
BspPI GGATC 4 cut(s) 814, 1181, 1292, 1358
BsrBI CCGCTC 1 cut(s) 357
BsrGI TGTACA 1 cut(s) 916
BsrI ACTGG 5 cut(s) 200, 276, 297, 351, 1373
BssECI CCNNGG 2 cut(s) 127, 966
BssMI GATC 6 cut(s) 71, 364, 819, 1186, 1284, 1363
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 4 cut(s) 22, 235, 496, 928
Bst6I CTCTTC 1 cut(s) 966
BstAUI TGTACA 1 cut(s) 916
BstC8I GCNNGC 3 cut(s) 150, 482, 770
BstDEI CTNAG 4 cut(s) 212, 353, 773, 1320
BstEII GGTNACC 1 cut(s) 332
BstF5I GGATG 4 cut(s) 36, 1104, 1175, 1404
BstFNI CGCG 2 cut(s) 375, 646
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 6 cut(s) 74, 367, 822, 1189, 1287, 1366
BstMAI GTCTC 2 cut(s) 221, 448
BstMBI GATC 6 cut(s) 71, 364, 819, 1186, 1284, 1363
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 481, 643, 774
BstNI CCWGG 1 cut(s) 93
BstPI GGTNACC 1 cut(s) 332
BstSCI CCNGG 4 cut(s) 91, 624, 1264, 1280
BstUI CGCG 2 cut(s) 375, 646
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 2 cut(s) 125, 229
BstX2I RGATCY 2 cut(s) 819, 1363
BstYI RGATCY 2 cut(s) 819, 1363
BsuRI GGCC 2 cut(s) 120, 198
BtsCI GGATG 4 cut(s) 36, 1104, 1175, 1404
BtsIMutI CAGTG 1 cut(s) 283
BveI ACCTGC 2 cut(s) 259, 842
Cac8I GCNNGC 3 cut(s) 150, 482, 770
CciI TCATGA 1 cut(s) 856
CfoI GCGC 1 cut(s) 375
Cfr13I GGNCC 5 cut(s) 197, 248, 439, 679, 1250
CseI GACGC 1 cut(s) 364
Csp6I GTAC 4 cut(s) 193, 917, 1006, 1376
CviAII CATG 7 cut(s) 145, 245, 476, 857, 981, 1011, 1329
CviQI GTAC 4 cut(s) 193, 917, 1006, 1376
DdeI CTNAG 4 cut(s) 212, 353, 773, 1320
DpnI GATC 6 cut(s) 73, 366, 821, 1188, 1286, 1365
DpnII GATC 6 cut(s) 71, 364, 819, 1186, 1284, 1363
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 966
EarI CTCTTC 1 cut(s) 966
Eco47I GGWCC 4 cut(s) 248, 439, 679, 1250
Eco57I CTGAAG 1 cut(s) 1339
Eco88I CYCGRG 1 cut(s) 407
Eco91I GGTNACC 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 1250
EcoO65I GGTNACC 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 91
EcoT22I ATGCAT 1 cut(s) 471
FaeI CATG 7 cut(s) 148, 248, 479, 860, 984, 1014, 1332
FaqI GGGAC 1 cut(s) 608
FatI CATG 7 cut(s) 144, 244, 475, 856, 980, 1010, 1328
FauNDI CATATG 1 cut(s) 1149
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 3 cut(s) 255, 358, 893
FokI GGATG 4 cut(s) 23, 1091, 1162, 1391
Fsp4HI GCNGC 3 cut(s) 255, 358, 893
FspBI CTAG 1 cut(s) 954
GlaI GCGC 1 cut(s) 374
GluI GCNGC 3 cut(s) 255, 358, 893
GsuI CTGGAG 2 cut(s) 75, 368
HaeIII GGCC 2 cut(s) 120, 198
HapII CCGG 6 cut(s) 337, 626, 1066, 1169, 1265, 1282
HgaI GACGC 1 cut(s) 364
HhaI GCGC 1 cut(s) 375
Hin1II CATG 7 cut(s) 148, 248, 479, 860, 984, 1014, 1332
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 3 cut(s) 302, 978, 1423
HindII GTYRAC 3 cut(s) 302, 978, 1423
HindIII AAGCTT 2 cut(s) 209, 1415
HinfI GANTC 4 cut(s) 183, 311, 410, 1144
HpaI GTTAAC 1 cut(s) 1423
HpaII CCGG 6 cut(s) 337, 626, 1066, 1169, 1265, 1282
HphI GGTGA 2 cut(s) 66, 344
Hpy166II GTNNAC 7 cut(s) 302, 323, 919, 978, 1008, 1326, 1423
Hpy188I TCNGA 6 cut(s) 140, 380, 709, 970, 1191, 1368
Hpy188III TCNNGA 6 cut(s) 400, 451, 857, 935, 1066, 1169
Hpy8I GTNNAC 7 cut(s) 302, 323, 919, 978, 1008, 1326, 1423
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 2 cut(s) 602, 649
HpyCH4III ACNGT 4 cut(s) 22, 235, 496, 928
HpyCH4V TGCA 7 cut(s) 59, 257, 469, 826, 1073, 1301, 1343
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 481, 643, 774
HpyF3I CTNAG 4 cut(s) 212, 353, 773, 1320
Hsp92II CATG 7 cut(s) 148, 248, 479, 860, 984, 1014, 1332
HspAI GCGC 1 cut(s) 373
Kpn2I TCCGGA 2 cut(s) 1065, 1168
KspAI GTTAAC 1 cut(s) 1423
Kzo9I GATC 6 cut(s) 71, 364, 819, 1186, 1284, 1363
LmnI GCTCC 3 cut(s) 349, 542, 1171
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 3 cut(s) 456, 1220, 1359
MaeI CTAG 1 cut(s) 954
MaeIII GTNAC 4 cut(s) 332, 411, 647, 1315
MalI GATC 6 cut(s) 73, 366, 821, 1188, 1286, 1365
MbiI CCGCTC 1 cut(s) 357
MboI GATC 6 cut(s) 71, 364, 819, 1186, 1284, 1363
MboII GAAGA 5 cut(s) 125, 229, 923, 956, 983
MflI RGATCY 2 cut(s) 819, 1363
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 1 cut(s) 632
MluI ACGCGT 1 cut(s) 644
MlyI GAGTC 3 cut(s) 320, 419, 1138
Mph1103I ATGCAT 1 cut(s) 471
MroI TCCGGA 2 cut(s) 1065, 1168
MseI TTAA 2 cut(s) 630, 1422
MslI CAYNNNNRTG 1 cut(s) 474
MspI CCGG 6 cut(s) 337, 626, 1066, 1169, 1265, 1282
MspR9I CCNGG 4 cut(s) 93, 626, 1266, 1282
MvaI CCWGG 1 cut(s) 93
MvnI CGCG 2 cut(s) 375, 646
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 481, 643, 774
NciI CCSGG 3 cut(s) 626, 1266, 1282
NdeI CATATG 1 cut(s) 1149
NdeII GATC 6 cut(s) 71, 364, 819, 1186, 1284, 1363
NlaIII CATG 7 cut(s) 148, 248, 479, 860, 984, 1014, 1332
NlaIV GGNNCC 3 cut(s) 87, 680, 1173
NmuCI GTSAC 2 cut(s) 332, 411
NsiI ATGCAT 1 cut(s) 471
PagI TCATGA 1 cut(s) 856
PcsI WCGNNNNNNNCGW 1 cut(s) 1284
PfeI GAWTC 1 cut(s) 183
PflMI CCANNNNNTGG 1 cut(s) 1116
PfoI TCCNGGA 1 cut(s) 1264
PkrI GCNGC 3 cut(s) 256, 359, 894
PleI GAGTC 3 cut(s) 319, 418, 1138
PpsI GAGTC 3 cut(s) 319, 418, 1138
PpuMI RGGWCCY 1 cut(s) 1250
Psp5II RGGWCCY 1 cut(s) 1250
Psp6I CCWGG 1 cut(s) 91
PspEI GGTNACC 1 cut(s) 332
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 3 cut(s) 87, 680, 1173
PspPI GGNCC 5 cut(s) 197, 248, 439, 679, 1250
PspPPI RGGWCCY 1 cut(s) 1250
PsuI RGATCY 2 cut(s) 819, 1363
RsaI GTAC 4 cut(s) 194, 918, 1007, 1377
RsaNI GTAC 4 cut(s) 193, 917, 1006, 1376
RseI CAYNNNNRTG 1 cut(s) 474
SaqAI TTAA 2 cut(s) 630, 1422
SatI GCNGC 3 cut(s) 255, 358, 893
Sau3AI GATC 6 cut(s) 71, 364, 819, 1186, 1284, 1363
Sau96I GGNCC 5 cut(s) 197, 248, 439, 679, 1250
SchI GAGTC 3 cut(s) 320, 419, 1138
ScrFI CCNGG 4 cut(s) 93, 626, 1266, 1282
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 3 cut(s) 456, 1220, 1359
SinI GGWCC 4 cut(s) 248, 439, 679, 1250
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 5 cut(s) 416, 461, 662, 725, 1383
SmoI CTYRAG 5 cut(s) 416, 461, 662, 725, 1383
Sse9I AATT 1 cut(s) 632
SsiI CCGC 3 cut(s) 159, 357, 892
SspI AATATT 1 cut(s) 1125
SspMI CTAG 1 cut(s) 954
StyD4I CCNGG 4 cut(s) 91, 624, 1264, 1280
TaaI ACNGT 4 cut(s) 22, 235, 496, 928
TaqI TCGA 5 cut(s) 186, 781, 1086, 1255, 1287
TaqII GACCGA 1 cut(s) 119
TasI AATT 1 cut(s) 632
TatI WGTACW 2 cut(s) 916, 1005
TauI GCSGC 2 cut(s) 360, 895
TfiI GAWTC 1 cut(s) 183
Tru1I TTAA 2 cut(s) 630, 1422
Tru9I TTAA 2 cut(s) 630, 1422
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 2 cut(s) 332, 411
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 2 cut(s) 332, 411
TspDTI ATGAA 8 cut(s) 17, 229, 233, 256, 524, 591, 855, 1016
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 1 cut(s) 283
Van91I CCANNNNNTGG 1 cut(s) 1116
VpaK11BI GGWCC 4 cut(s) 248, 439, 679, 1250
XmiI GTMKAC 1 cut(s) 322
XspI CTAG 1 cut(s) 954
Zsp2I ATGCAT 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.