Rw1G033330

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000704
Physical Location & Seq
Forward (+)
61425514 .. 61427571
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033330.1

Sequence Viewer

Length: 1443 bp
ATGGAAGATCTCTCGTTGATGATACTAGTTTTGACCCTCATATCACTTTTTTCTGGGTATTTTATCAAGTTTAAAACATCACTAGGGAAGAAACTTCCACCAGGGTCATTTGGTATTCCGTTAATTGGAGAGTCCATAAGCTTCATCCGAGCACAAATACAGGACGGATCCGATGAGTGGATCCGGTCCCGTATCCTCAAGTATGGACCAGTCTTCAAGACCTCGCTCATGGGATCTAAAACGGTGGTTATAACTGGCCAAGCTGGAAACCGGTTTGTGTTTGGTGGAGGTGACACTGGTATTGGAGGCAACCAAGTAGCAACTGTAGCCAGAATTCTCGGAAAGCACAGCATATTTGAGATTTCTGGGTTTAGGCACAAGCTTGTGAGGAGTGCCATAACCAACTTCCTAAAGCCAGAAAACATTCAAAGAATTGTAGGCGAAATCGATTTGTTAGTCAAAGACCAAATTTTCAAGGAACTCGATGGCAAGGATGTAGTACAATTAGTGCCTTTGGTGAAGAGATTAACATTCAAGGTTACTTGCAGTTTGTTTTTTGGACTACCAGAAGGCAAAGACCAAGATACATTGTTTGAAGACTTTACTATTGCCATCAAGGGTCTGTGGGCAATTCCATTAAACCTTCTAGGCACTCAATTTTACAAAGCAGTGCAAGCACGTGGCAGGATAGCCAAGCTCCTCACCAAGCTCATCAAGGAAAAGAAGAATAAGGCAACAAAAATGACCCCCCAGAACAATGACATCATCTCAGCCCTGCTTCTTCTGATAGATGAAGTTGGTAAGCCTCTGCAAGAGCAAGAGATTGTTGATAATCTCATCACTGTGATAGTTGCTAGCCATGACACCACCTCAATTCTCCTGAGCCTCCTTTTAAGGCATCTTTCTACAGATTCCAAGATTTTCAGTGAGGTCCTTCAAGAGCAAAAAGAAGTAGTTAAAGCTGTTGAAGGAAGTGAAAGAAAGGCCATTACATGGAATGAAATCCAAATGATGAAGCACACATGGAGAGTGGCCCAAGAGCTCATGAGGATGACTCCCCCCGCTTTTGGTAACTTCAAATGTGCATGGAGAGATACTAACTTCGATGGCTATGATATCCCCAAGGGATGGAAGGTTTTCTGGGTGGCATCTGGTACACACATGGACAGCAACATATTTGAAGATCCAAACAGGTTTGATCCATCGCGATTCGAGAACTCAACGAAATCATTTCCTCCGTACACATACATTGCATTCGGAGCAGGCCCGCGAATTTGTCCTGGGGCAGAATTTGCAAGGATTGAAGTGCTGTTAACGATTCATCATTTGTTAACAAAGTACTCATGGACGCAGATGATCAAGGACGAGCCTTTAAGACGTGAACCCTTGCCTTATCCAGCCATGGGACTTCCGGTCAAACTTCACCAAAGGAACGATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

480

Amino Acids

54.13

Weight (kDa)

9.32

Isoelectric Point (pI)

27.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 462 7.5e-62 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
AccB7I CCANNNNNTGG 2 cut(s) 993, 1128
AccII CGCG 2 cut(s) 1207, 1270
AciI CCGC 2 cut(s) 1062, 1268
AclWI GGATC 7 cut(s) 162, 175, 175, 188, 241, 1178, 1193
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 4 cut(s) 333, 468, 1272, 1289
AcvI CACGTG 1 cut(s) 680
AfaI GTAC 4 cut(s) 501, 1156, 1241, 1340
AfiI CCNNNNNNNGG 6 cut(s) 125, 177, 993, 1067, 1128, 1403
AgeI ACCGGT 1 cut(s) 270
AhdI GACNNNNNGTC 1 cut(s) 1412
AhlI ACTAGT 1 cut(s) 25
AjiI CACGTC 1 cut(s) 1379
AjnI CCWGG 2 cut(s) 100, 1279
AluBI AGCT 7 cut(s) 141, 263, 382, 697, 709, 962, 1042
AluI AGCT 7 cut(s) 141, 263, 382, 697, 709, 962, 1042
Alw21I GWGCWC 2 cut(s) 154, 1044
AlwI GGATC 7 cut(s) 162, 175, 175, 188, 241, 1178, 1193
AoxI GGCC 4 cut(s) 256, 984, 1032, 1264
ApoI RAATTY 4 cut(s) 333, 468, 1272, 1289
AsiGI ACCGGT 1 cut(s) 270
Asp700I GAANNNNTTC 2 cut(s) 423, 1136
AspS9I GGNCC 5 cut(s) 186, 206, 931, 1033, 1265
AsuHPI GGTGA 4 cut(s) 302, 529, 694, 1415
AsuNHI GCTAGC 1 cut(s) 854
AvaII GGWCC 3 cut(s) 186, 206, 931
BalI TGGCCA 1 cut(s) 258
BamHI GGATCC 2 cut(s) 167, 180
BanII GRGCYC 1 cut(s) 1044
BbrPI CACGTG 1 cut(s) 680
BbsI GAAGAC 2 cut(s) 205, 603
Bbv12I GWGCWC 2 cut(s) 154, 1044
BccI CCATC 5 cut(s) 479, 620, 1100, 1122, 1210
BciT130I CCWGG 2 cut(s) 102, 1281
BciVI GTATCC 1 cut(s) 203
BclI TGATCA 1 cut(s) 1356
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 4 cut(s) 26, 83, 647, 855
BfmI CTRYAG 2 cut(s) 324, 906
BfuI GTATCC 1 cut(s) 203
BglII AGATCT 1 cut(s) 7
BmcAI AGTACT 1 cut(s) 1340
Bme1390I CCNGG 2 cut(s) 102, 1281
Bme18I GGWCC 3 cut(s) 186, 206, 931
BmeRI GACNNNNNGTC 1 cut(s) 1412
BmgBI CACGTC 1 cut(s) 1379
BmgT120I GGNCC 5 cut(s) 186, 206, 931, 1033, 1265
BmiI GGNNCC 3 cut(s) 169, 182, 188
BmrFI CCNGG 2 cut(s) 102, 1281
BmsI GCATC 2 cut(s) 907, 1157
BmtI GCTAGC 1 cut(s) 858
BpiI GAAGAC 2 cut(s) 205, 603
BplI GAGNNNNNCTC 2 cut(s) 1039, 1071
Bpu10I CCTNAGC 1 cut(s) 881
BpuEI CTTGAG 1 cut(s) 182
Bsa29I ATCGAT 1 cut(s) 447
BsaAI YACGTR 1 cut(s) 680
BsaJI CCNNGG 4 cut(s) 101, 1122, 1280, 1401
BsaWI WCCGGW 3 cut(s) 183, 270, 1411
Bsc4I CCNNNNNNNGG 6 cut(s) 125, 177, 993, 1067, 1128, 1403
Bse118I RCCGGY 1 cut(s) 270
Bse1I ACTGG 3 cut(s) 209, 259, 301
Bse3DI GCAATG 1 cut(s) 1248
BseBI CCWGG 2 cut(s) 102, 1281
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 4 cut(s) 101, 1122, 1280, 1401
BseGI GGATG 4 cut(s) 144, 499, 1056, 1133
BseLI CCNNNNNNNGG 6 cut(s) 125, 177, 993, 1067, 1128, 1403
BseMI GCAATG 1 cut(s) 1248
BseMII CTCAG 2 cut(s) 783, 872
BseNI ACTGG 3 cut(s) 209, 259, 301
BseRI GAGGAG 2 cut(s) 403, 689
Bsh1236I CGCG 2 cut(s) 1207, 1270
BshFI GGCC 4 cut(s) 258, 986, 1034, 1266
BshTI ACCGGT 1 cut(s) 270
BshVI ATCGAT 1 cut(s) 447
BsiHKAI GWGCWC 2 cut(s) 154, 1044
BsiSI CCGG 3 cut(s) 184, 271, 1412
BslFI GGGAC 2 cut(s) 172, 1419
BslI CCNNNNNNNGG 6 cut(s) 125, 177, 993, 1067, 1128, 1403
BsmFI GGGAC 2 cut(s) 172, 1419
BsmI GAATGC 1 cut(s) 1253
BsnI GGCC 4 cut(s) 258, 986, 1034, 1266
Bsp1286I GDGCHC 2 cut(s) 154, 1044
Bsp143I GATC 7 cut(s) 7, 167, 180, 233, 1183, 1198, 1356
Bsp19I CCATGG 1 cut(s) 1401
Bsp68I TCGCGA 1 cut(s) 1207
BspACI CCGC 2 cut(s) 1062, 1268
BspANI GGCC 4 cut(s) 258, 986, 1034, 1266
BspCNI CTCAG 2 cut(s) 782, 873
BspDI ATCGAT 1 cut(s) 447
BspFNI CGCG 2 cut(s) 1207, 1270
BspHI TCATGA 1 cut(s) 1044
BspLI GGNNCC 3 cut(s) 169, 182, 188
BspOI GCTAGC 1 cut(s) 858
BspPI GGATC 7 cut(s) 162, 175, 175, 188, 241, 1178, 1193
BsrDI GCAATG 1 cut(s) 1248
BsrFI RCCGGY 1 cut(s) 270
BsrI ACTGG 3 cut(s) 209, 259, 301
BssAI RCCGGY 1 cut(s) 270
BssECI CCNNGG 4 cut(s) 101, 1122, 1280, 1401
BssMI GATC 7 cut(s) 7, 167, 180, 233, 1183, 1198, 1356
BssT1I CCWWGG 2 cut(s) 1122, 1401
Bst2UI CCWGG 2 cut(s) 102, 1281
Bst4CI ACNGT 3 cut(s) 244, 325, 844
Bst6I CTCTTC 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 1292
BstBAI YACGTR 1 cut(s) 680
BstC8I GCNNGC 4 cut(s) 675, 856, 1264, 1268
BstDEI CTNAG 2 cut(s) 769, 881
BstDSI CCRYGG 1 cut(s) 1401
BstF5I GGATG 4 cut(s) 144, 499, 1056, 1133
BstFNI CGCG 2 cut(s) 1207, 1270
BstKTI GATC 7 cut(s) 10, 170, 183, 236, 1186, 1201, 1359
BstMBI GATC 7 cut(s) 7, 167, 180, 233, 1183, 1198, 1356
BstMWI GCNNNNNNNGC 4 cut(s) 326, 674, 1259, 1292
BstNI CCWGG 2 cut(s) 102, 1281
BstSCI CCNGG 2 cut(s) 100, 1279
BstSFI CTRYAG 2 cut(s) 324, 906
BstUI CGCG 2 cut(s) 1207, 1270
BstV2I GAAGAC 2 cut(s) 205, 603
BstX2I RGATCY 5 cut(s) 7, 167, 180, 233, 1183
BstYI RGATCY 5 cut(s) 7, 167, 180, 233, 1183
Bsu15I ATCGAT 1 cut(s) 447
BsuI GTATCC 1 cut(s) 203
BsuRI GGCC 4 cut(s) 258, 986, 1034, 1266
BsuTUI ATCGAT 1 cut(s) 447
BtgI CCRYGG 1 cut(s) 1401
BtgZI GCGATG 1 cut(s) 1188
BtrI CACGTC 1 cut(s) 1379
BtsCI GGATG 4 cut(s) 144, 499, 1056, 1133
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 4 cut(s) 294, 675, 840, 931
BtuMI TCGCGA 1 cut(s) 1207
Cac8I GCNNGC 4 cut(s) 675, 856, 1264, 1268
CciI TCATGA 1 cut(s) 1044
Cfr10I RCCGGY 1 cut(s) 270
Cfr13I GGNCC 5 cut(s) 186, 206, 931, 1033, 1265
ClaI ATCGAT 1 cut(s) 447
CseI GACGC 1 cut(s) 1357
Csp6I GTAC 4 cut(s) 500, 1155, 1240, 1339
CspAI ACCGGT 1 cut(s) 270
CviAII CATG 9 cut(s) 229, 860, 993, 1023, 1045, 1086, 1162, 1344, 1402
CviQI GTAC 4 cut(s) 500, 1155, 1240, 1339
DdeI CTNAG 2 cut(s) 769, 881
DpnI GATC 7 cut(s) 9, 169, 182, 235, 1185, 1200, 1358
DpnII GATC 7 cut(s) 7, 167, 180, 233, 1183, 1198, 1356
DraI TTTAAA 1 cut(s) 73
DriI GACNNNNNGTC 1 cut(s) 1412
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 515
Eam1105I GACNNNNNGTC 1 cut(s) 1412
EarI CTCTTC 1 cut(s) 515
Ecl136II GAGCTC 1 cut(s) 1042
Eco130I CCWWGG 2 cut(s) 1122, 1401
Eco24I GRGCYC 1 cut(s) 1044
Eco32I GATATC 1 cut(s) 1117
Eco47I GGWCC 3 cut(s) 186, 206, 931
Eco53kI GAGCTC 1 cut(s) 1042
Eco72I CACGTG 1 cut(s) 680
EcoICRI GAGCTC 1 cut(s) 1042
EcoO109I RGGNCCY 1 cut(s) 931
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 2 cut(s) 100, 1279
EcoRV GATATC 1 cut(s) 1117
EcoT14I CCWWGG 2 cut(s) 1122, 1401
EcoT38I GRGCYC 1 cut(s) 1044
ErhI CCWWGG 2 cut(s) 1122, 1401
FaeI CATG 9 cut(s) 232, 863, 996, 1026, 1048, 1089, 1165, 1347, 1405
FaqI GGGAC 2 cut(s) 172, 1419
FatI CATG 9 cut(s) 228, 859, 992, 1022, 1044, 1085, 1161, 1343, 1401
FauI CCCGC 2 cut(s) 1069, 1275
FbaI TGATCA 1 cut(s) 1356
FokI GGATG 4 cut(s) 131, 506, 1063, 1140
FriOI GRGCYC 1 cut(s) 1044
FspBI CTAG 4 cut(s) 26, 83, 647, 855
HaeIII GGCC 4 cut(s) 258, 986, 1034, 1266
HapII CCGG 3 cut(s) 184, 271, 1412
HgaI GACGC 1 cut(s) 1357
Hin1II CATG 9 cut(s) 232, 863, 996, 1026, 1048, 1089, 1165, 1347, 1405
HincII GTYRAC 2 cut(s) 1314, 1332
HindII GTYRAC 2 cut(s) 1314, 1332
HindIII AAGCTT 2 cut(s) 139, 380
HinfI GANTC 5 cut(s) 131, 911, 1054, 1209, 1318
HpaI GTTAAC 2 cut(s) 1314, 1332
HpaII CCGG 3 cut(s) 184, 271, 1412
HphI GGTGA 4 cut(s) 302, 529, 694, 1415
Hpy166II GTNNAC 5 cut(s) 1157, 1242, 1314, 1332, 1382
Hpy188I TCNGA 5 cut(s) 149, 172, 341, 786, 1259
Hpy188III TCNNGA 6 cut(s) 217, 880, 938, 1045, 1206, 1213
Hpy8I GTNNAC 5 cut(s) 1157, 1242, 1314, 1332, 1382
HpyAV CCTTC 5 cut(s) 563, 653, 944, 962, 1126
HpyCH4III ACNGT 3 cut(s) 244, 325, 844
HpyCH4IV ACGT 2 cut(s) 679, 1378
HpyCH4V TGCA 6 cut(s) 546, 673, 811, 1085, 1253, 1295
HpyF10VI GCNNNNNNNGC 4 cut(s) 326, 674, 1259, 1292
HpyF3I CTNAG 2 cut(s) 769, 881
HpySE526I ACGT 2 cut(s) 679, 1378
Hsp92II CATG 9 cut(s) 232, 863, 996, 1026, 1048, 1089, 1165, 1347, 1405
Ksp22I TGATCA 1 cut(s) 1356
KspAI GTTAAC 2 cut(s) 1314, 1332
Kzo9I GATC 7 cut(s) 7, 167, 180, 233, 1183, 1198, 1356
LmnI GCTCC 2 cut(s) 702, 1259
LweI GCATC 2 cut(s) 907, 1157
MaeI CTAG 4 cut(s) 26, 83, 647, 855
MaeII ACGT 2 cut(s) 679, 1378
MaeIII GTNAC 3 cut(s) 290, 538, 1070
MalI GATC 7 cut(s) 9, 169, 182, 235, 1185, 1200, 1358
MboI GATC 7 cut(s) 7, 167, 180, 233, 1183, 1198, 1356
MboII GAAGA 8 cut(s) 17, 100, 205, 532, 608, 736, 773, 1193
MflI RGATCY 5 cut(s) 7, 167, 180, 233, 1183
MhlI GDGCHC 2 cut(s) 154, 1044
MlsI TGGCCA 1 cut(s) 258
MluNI TGGCCA 1 cut(s) 258
MlyI GAGTC 2 cut(s) 140, 1048
Mox20I TGGCCA 1 cut(s) 258
MroXI GAANNNNTTC 2 cut(s) 423, 1136
MscI TGGCCA 1 cut(s) 258
MseI TTAA 9 cut(s) 72, 122, 527, 638, 893, 957, 1313, 1331, 1373
MslI CAYNNNNRTG 2 cut(s) 842, 1049
Msp20I TGGCCA 1 cut(s) 258
MspI CCGG 3 cut(s) 184, 271, 1412
MspR9I CCNGG 2 cut(s) 102, 1281
Mva1269I GAATGC 1 cut(s) 1253
MvaI CCWGG 2 cut(s) 102, 1281
MvnI CGCG 2 cut(s) 1207, 1270
MwoI GCNNNNNNNGC 4 cut(s) 326, 674, 1259, 1292
NcoI CCATGG 1 cut(s) 1401
NdeII GATC 7 cut(s) 7, 167, 180, 233, 1183, 1198, 1356
NheI GCTAGC 1 cut(s) 854
NlaIII CATG 9 cut(s) 232, 863, 996, 1026, 1048, 1089, 1165, 1347, 1405
NlaIV GGNNCC 3 cut(s) 169, 182, 188
NmuCI GTSAC 1 cut(s) 290
NruI TCGCGA 1 cut(s) 1207
PagI TCATGA 1 cut(s) 1044
PctI GAATGC 1 cut(s) 1253
PdmI GAANNNNTTC 2 cut(s) 423, 1136
PfeI GAWTC 3 cut(s) 911, 1209, 1318
PflMI CCANNNNNTGG 2 cut(s) 993, 1128
PinAI ACCGGT 1 cut(s) 270
PleI GAGTC 2 cut(s) 139, 1048
PmaCI CACGTG 1 cut(s) 680
PmlI CACGTG 1 cut(s) 680
PpsI GAGTC 2 cut(s) 139, 1048
Ppu21I YACGTR 1 cut(s) 680
PpuMI RGGWCCY 1 cut(s) 931
PsiI TTATAA 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 1044
Psp5II RGGWCCY 1 cut(s) 931
Psp6I CCWGG 2 cut(s) 100, 1279
PspCI CACGTG 1 cut(s) 680
PspGI CCWGG 2 cut(s) 100, 1279
PspN4I GGNNCC 3 cut(s) 169, 182, 188
PspPI GGNCC 5 cut(s) 186, 206, 931, 1033, 1265
PspPPI RGGWCCY 1 cut(s) 931
PsuI RGATCY 5 cut(s) 7, 167, 180, 233, 1183
RruI TCGCGA 1 cut(s) 1207
RsaI GTAC 4 cut(s) 501, 1156, 1241, 1340
RsaNI GTAC 4 cut(s) 500, 1155, 1240, 1339
RseI CAYNNNNRTG 2 cut(s) 842, 1049
SacI GAGCTC 1 cut(s) 1044
SaqAI TTAA 9 cut(s) 72, 122, 527, 638, 893, 957, 1313, 1331, 1373
Sau3AI GATC 7 cut(s) 7, 167, 180, 233, 1183, 1198, 1356
Sau96I GGNCC 5 cut(s) 186, 206, 931, 1033, 1265
ScaI AGTACT 1 cut(s) 1340
SchI GAGTC 2 cut(s) 140, 1048
ScrFI CCNGG 2 cut(s) 102, 1281
SduI GDGCHC 2 cut(s) 154, 1044
SfaNI GCATC 2 cut(s) 907, 1157
SfcI CTRYAG 2 cut(s) 324, 906
SinI GGWCC 3 cut(s) 186, 206, 931
SmiMI CAYNNNNRTG 2 cut(s) 842, 1049
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SpeI ACTAGT 1 cut(s) 25
SsiI CCGC 2 cut(s) 1062, 1268
SspMI CTAG 4 cut(s) 26, 83, 647, 855
SstI GAGCTC 1 cut(s) 1044
StyD4I CCNGG 2 cut(s) 100, 1279
StyI CCWWGG 2 cut(s) 1122, 1401
TaaI ACNGT 3 cut(s) 244, 325, 844
TaiI ACGT 2 cut(s) 682, 1381
TaqI TCGA 4 cut(s) 447, 483, 1104, 1212
TatI WGTACW 2 cut(s) 499, 1338
TfiI GAWTC 3 cut(s) 911, 1209, 1318
Tru1I TTAA 9 cut(s) 72, 122, 527, 638, 893, 957, 1313, 1331, 1373
Tru9I TTAA 9 cut(s) 72, 122, 527, 638, 893, 957, 1313, 1331, 1373
TscAI CASTG 4 cut(s) 301, 675, 847, 931
TseFI GTSAC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 5 cut(s) 133, 807, 1014, 1028, 1310
TspGWI ACGGA 3 cut(s) 108, 180, 1227
TspRI CASTG 4 cut(s) 301, 675, 847, 931
Van91I CCANNNNNTGG 2 cut(s) 993, 1128
VpaK11BI GGWCC 3 cut(s) 186, 206, 931
XapI RAATTY 4 cut(s) 333, 468, 1272, 1289
XmnI GAANNNNTTC 2 cut(s) 423, 1136
XspI CTAG 4 cut(s) 26, 83, 647, 855
ZrmI AGTACT 1 cut(s) 1340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.