FvH4_5g35780

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000704
Physical Location & Seq
Forward (+)
26196514 .. 26198375
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35780.t1

Sequence Viewer

Length: 1422 bp
ATGAACACCCTCTTCGCAGTCTTCCTCTTCCTCATTCCCATATTCCTTCTCCATACAAGAAGAAAAAAGTCATCGGAAAGACTTCCGCCGGGTTCATTAGGACTACCCCTGATAGGGCAGAGCCTTGGTCTTCTTCGAGCCATGCGTGCCAACACTGCTGAGAAATGGCTTGAACAAAGGATCAAAAAGTACGGTCCAGTTTCAAAGCTAAGCCTCTTTGGCAAGCCAACAGTCTTTATCCATGGACAAGCTGCAAACAAGTTTGTATTCACCGCTAACGGCAGCACCATTTCCAGCAAACAGACTGAGTCTATTCGTAGAATTTTAGGGGAACGGAGTTTATTTGAGCTGAGTGCCGAGGATCACAAGCGTGTGAGAAGTGCTCTTATGCTGTTCTTAAAGCCTGAGTCACTGAAGCAATATGTGGAGAAAATGGATGAAGAAATCAGGAACCACCTTGAAATGCATTGGCATGGAAAACAACAAGTAACAGTCTTGCCCCTGATGAAGAACCTTACTTTCAACATAATATGCTCTCTACTGTTTGGTCTTGAACGGGGAACTCGCAGAGACGAATTCGTGGAGTGTTTCCAAACAATGATGGAAGGAATGTGGTCAGTGCCAGTTAACTTGCCGTTCACGCGTTACAACAGCAGCCTCAAGGCAAGCAAGAGGGTTCAAAACATGCTTAAGGAACTTATACGCGAAAAGAGGGTACAGCTTGAGCAAAGAAAAGCTTCTCCACAACAAGACCTCGTCACTTGCTTCCTCAGCATCCGAAATGATGAAGGTAAAGAAGAATTAACAGAACTGGAGATGGTGCACAATGTCATGGTAGTCATGGTTGCAGGACATGACACTTCATCGATTCTGATTACGTTCCTTTTGCGGCTTCTAGCTAATGAACCAGCTGTTTATGCACACCTTCTTCAAGAGCAGGAAGAAATAGCTAGAAGCAAATCGGCAGGAGAGTTTCTAACATGGGAAGACCTTGCCAAAATGAAATACACATGGAGAGTAGCACAGGAAACTCTGAGGGTTACTCCTCCTATCTTTGGTAGCTTCAGAACGGCTATGAAAGATATCGAGTTTGGTGGATATCTCATTCCCAAAGGGTGGCAGATATTTTGGGCTGCCCATATGACCCACATGAATGATAGTATATTCCCAGAACCATCCAAGTTTGATCCTAGTAGGTTTGAGAAGCAAGTACTTCCACCCTACTGCTTTGTTGGATTTGGAGGAGGACCTCGAATATGCCCAGGGTATGAGTTTGCCAGAATTGAAACCCTTATTGCTATGCACCATATGGTAACTCGATTCACTTGGAAGCTAACTGCAGATGATCAGTTCAGTAGGGATCCAATGCCAGTGCCTACTCAAGGACTACCCCTTGAAATCATGCCAAGAACACGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

474

Amino Acids

54.54

Weight (kDa)

9.4

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 29 - 450 5.5e-76 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1116
AccII CGCG 2 cut(s) 643, 705
AciI CCGC 3 cut(s) 86, 273, 889
AclWI GGATC 5 cut(s) 188, 369, 1181, 1355, 1368
AcsI RAATTY 2 cut(s) 321, 575
AcuI CTGAAG 2 cut(s) 434, 1048
AfaI GTAC 3 cut(s) 191, 717, 1212
AfiI CCNNNNNNNGG 5 cut(s) 113, 114, 1055, 1116, 1382
AflII CTTAAG 1 cut(s) 689
AflIII ACRYGT 1 cut(s) 641
AgsI TTSAA 9 cut(s) 173, 204, 461, 523, 554, 680, 932, 1286, 1397
AjnI CCWGG 1 cut(s) 1261
AleI CACNNNNGTG 1 cut(s) 369
Alw21I GWGCWC 2 cut(s) 385, 825
Alw26I GTCTC 1 cut(s) 564
Alw44I GTGCAC 1 cut(s) 821
AlwI GGATC 5 cut(s) 188, 369, 1181, 1355, 1368
ApaLI GTGCAC 1 cut(s) 821
ApeKI GCWGC 4 cut(s) 251, 282, 654, 1133
ApoI RAATTY 2 cut(s) 321, 575
Asp700I GAANNNNTTC 2 cut(s) 81, 736
AspS9I GGNCC 2 cut(s) 194, 1247
AsuC2I CCSGG 1 cut(s) 90
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 2 cut(s) 194, 1247
BaeGI GKGCMC 1 cut(s) 825
BamHI GGATCC 1 cut(s) 1360
BbsI GAAGAC 3 cut(s) 13, 122, 993
Bbv12I GWGCWC 2 cut(s) 385, 825
BbvCI CCTCAGC 1 cut(s) 770
BbvI GCAGC 4 cut(s) 238, 294, 666, 1120
BccI CCATC 3 cut(s) 595, 811, 1183
BceAI ACGGC 3 cut(s) 295, 619, 1086
BciT130I CCWGG 1 cut(s) 1263
BclI TGATCA 1 cut(s) 1345
BcnI CCSGG 1 cut(s) 90
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 3 cut(s) 896, 951, 1191
BfmI CTRYAG 1 cut(s) 1338
BfrI CTTAAG 1 cut(s) 689
BglI GCCNNNNNGGC 1 cut(s) 219
BisI GCNGC 5 cut(s) 252, 283, 655, 890, 1134
BlpI GCTNAGC 1 cut(s) 209
BlsI GCNGC 5 cut(s) 253, 284, 656, 891, 1135
BmcAI AGTACT 1 cut(s) 1212
Bme1390I CCNGG 2 cut(s) 90, 1263
Bme18I GGWCC 2 cut(s) 194, 1247
BmgT120I GGNCC 2 cut(s) 194, 1247
BmiI GGNNCC 2 cut(s) 452, 1362
BmrFI CCNGG 2 cut(s) 90, 1263
BmsI GCATC 1 cut(s) 783
BpiI GAAGAC 3 cut(s) 13, 122, 993
BplI GAGNNNNNCTC 4 cut(s) 367, 399, 1027, 1059
BpmI CTGGAG 1 cut(s) 833
Bpu10I CCTNAGC 1 cut(s) 770
Bpu1102I GCTNAGC 1 cut(s) 209
BpuEI CTTGAG 3 cut(s) 644, 743, 1365
BpuMI CCSGG 1 cut(s) 90
Bsa29I ATCGAT 1 cut(s) 866
BsaJI CCNNGG 5 cut(s) 124, 241, 357, 1261, 1262
Bsc4I CCNNNNNNNGG 5 cut(s) 113, 114, 1055, 1116, 1382
Bse1I ACTGG 4 cut(s) 197, 623, 816, 1370
BseBI CCWGG 1 cut(s) 1263
BseCI ATCGAT 1 cut(s) 866
BseDI CCNNGG 5 cut(s) 124, 241, 357, 1261, 1262
BseGI GGATG 3 cut(s) 442, 774, 1175
BseLI CCNNNNNNNGG 5 cut(s) 113, 114, 1055, 1116, 1382
BseMII CTCAG 6 cut(s) 150, 297, 341, 396, 784, 1025
BseNI ACTGG 4 cut(s) 197, 623, 816, 1370
BseRI GAGGAG 2 cut(s) 1035, 1257
BseSI GKGCMC 1 cut(s) 825
BseXI GCAGC 4 cut(s) 238, 294, 666, 1120
Bsh1236I CGCG 2 cut(s) 643, 705
BshVI ATCGAT 1 cut(s) 866
BsiHKAI GWGCWC 2 cut(s) 385, 825
BsiSI CCGG 1 cut(s) 89
BslI CCNNNNNNNGG 5 cut(s) 113, 114, 1055, 1116, 1382
BsmAI GTCTC 1 cut(s) 564
BsmBI CGTCTC 1 cut(s) 564
Bsp1286I GDGCHC 2 cut(s) 385, 825
Bsp143I GATC 5 cut(s) 180, 361, 1186, 1345, 1360
Bsp1720I GCTNAGC 1 cut(s) 209
Bsp19I CCATGG 1 cut(s) 241
BspACI CCGC 3 cut(s) 86, 273, 889
BspCNI CTCAG 6 cut(s) 151, 298, 342, 397, 783, 1026
BspDI ATCGAT 1 cut(s) 866
BspFNI CGCG 2 cut(s) 643, 705
BspLI GGNNCC 2 cut(s) 452, 1362
BspMAI CTGCAG 1 cut(s) 1342
BspPI GGATC 5 cut(s) 188, 369, 1181, 1355, 1368
BspTI CTTAAG 1 cut(s) 689
BsrI ACTGG 4 cut(s) 197, 623, 816, 1370
BssECI CCNNGG 5 cut(s) 124, 241, 357, 1261, 1262
BssMI GATC 5 cut(s) 180, 361, 1186, 1345, 1360
BssT1I CCWWGG 2 cut(s) 124, 241
Bst2UI CCWGG 1 cut(s) 1263
Bst4CI ACNGT 4 cut(s) 194, 232, 493, 543
Bst6I CTCTTC 2 cut(s) 17, 32
BstAFI CTTAAG 1 cut(s) 689
BstC8I GCNNGC 3 cut(s) 147, 224, 667
BstDEI CTNAG 7 cut(s) 159, 209, 306, 350, 405, 770, 1034
BstDSI CCRYGG 1 cut(s) 241
BstENI CCTNNNNNAGG 1 cut(s) 1380
BstF5I GGATG 3 cut(s) 442, 774, 1175
BstFNI CGCG 2 cut(s) 643, 705
BstKTI GATC 5 cut(s) 183, 364, 1189, 1348, 1363
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 5 cut(s) 180, 361, 1186, 1345, 1360
BstMWI GCNNNNNNNGC 6 cut(s) 146, 155, 219, 640, 771, 917
BstNI CCWGG 1 cut(s) 1263
BstNSI RCATGY 1 cut(s) 688
BstSCI CCNGG 2 cut(s) 88, 1261
BstSFI CTRYAG 1 cut(s) 1338
BstSLI GKGCMC 1 cut(s) 825
BstUI CGCG 2 cut(s) 643, 705
BstV1I GCAGC 4 cut(s) 238, 294, 666, 1120
BstV2I GAAGAC 3 cut(s) 13, 122, 993
BstX2I RGATCY 1 cut(s) 1360
BstYI RGATCY 1 cut(s) 1360
Bsu15I ATCGAT 1 cut(s) 866
BsuTUI ATCGAT 1 cut(s) 866
BtgI CCRYGG 1 cut(s) 241
BtsCI GGATG 3 cut(s) 442, 774, 1175
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 4 cut(s) 153, 410, 624, 1377
Cac8I GCNNGC 3 cut(s) 147, 224, 667
Cfr13I GGNCC 2 cut(s) 194, 1247
ClaI ATCGAT 1 cut(s) 866
Csp6I GTAC 3 cut(s) 190, 716, 1211
CviQI GTAC 3 cut(s) 190, 716, 1211
DdeI CTNAG 7 cut(s) 159, 209, 306, 350, 405, 770, 1034
DpnI GATC 5 cut(s) 182, 363, 1188, 1347, 1362
DpnII GATC 5 cut(s) 180, 361, 1186, 1345, 1360
Eam1104I CTCTTC 2 cut(s) 17, 32
EarI CTCTTC 2 cut(s) 17, 32
EciI GGCGGA 1 cut(s) 75
Eco130I CCWWGG 2 cut(s) 124, 241
Eco32I GATATC 2 cut(s) 1084, 1100
Eco47I GGWCC 2 cut(s) 194, 1247
Eco57I CTGAAG 2 cut(s) 434, 1048
EcoNI CCTNNNNNAGG 1 cut(s) 1380
EcoO109I RGGNCCY 1 cut(s) 1247
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 1 cut(s) 1261
EcoRV GATATC 2 cut(s) 1084, 1100
EcoT14I CCWWGG 2 cut(s) 124, 241
EcoT22I ATGCAT 1 cut(s) 468
ErhI CCWWGG 2 cut(s) 124, 241
Esp3I CGTCTC 1 cut(s) 564
FalI AAGNNNNNCTT 2 cut(s) 721, 753
FauNDI CATATG 2 cut(s) 1140, 1308
FbaI TGATCA 1 cut(s) 1345
Fnu4HI GCNGC 5 cut(s) 252, 283, 655, 890, 1134
FokI GGATG 3 cut(s) 449, 761, 1162
Fsp4HI GCNGC 5 cut(s) 252, 283, 655, 890, 1134
FspBI CTAG 3 cut(s) 896, 951, 1191
GluI GCNGC 5 cut(s) 252, 283, 655, 890, 1134
GsuI CTGGAG 1 cut(s) 833
HapII CCGG 1 cut(s) 89
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HindIII AAGCTT 1 cut(s) 735
HinfI GANTC 4 cut(s) 308, 407, 868, 1320
HpaI GTTAAC 1 cut(s) 628
HpaII CCGG 1 cut(s) 89
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 3 cut(s) 628, 639, 823
Hpy188I TCNGA 5 cut(s) 76, 779, 873, 1035, 1067
Hpy188III TCNNGA 3 cut(s) 448, 551, 932
Hpy8I GTNNAC 3 cut(s) 628, 639, 823
HpyAV CCTTC 4 cut(s) 56, 599, 782, 935
HpyCH4III ACNGT 4 cut(s) 194, 232, 493, 543
HpyCH4IV ACGT 1 cut(s) 878
HpyCH4V TGCA 7 cut(s) 254, 466, 823, 848, 920, 1303, 1340
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 155, 219, 640, 771, 917
HpyF3I CTNAG 7 cut(s) 159, 209, 306, 350, 405, 770, 1034
HpySE526I ACGT 1 cut(s) 878
Ksp22I TGATCA 1 cut(s) 1345
KspAI GTTAAC 1 cut(s) 628
Kzo9I GATC 5 cut(s) 180, 361, 1186, 1345, 1360
Lsp1109I GCAGC 4 cut(s) 238, 294, 666, 1120
LweI GCATC 1 cut(s) 783
MaeI CTAG 3 cut(s) 896, 951, 1191
MaeII ACGT 1 cut(s) 878
MaeIII GTNAC 6 cut(s) 408, 487, 644, 757, 1039, 1312
MalI GATC 5 cut(s) 182, 363, 1188, 1347, 1362
MboI GATC 5 cut(s) 180, 361, 1186, 1345, 1360
MflI RGATCY 1 cut(s) 1360
MhlI GDGCHC 2 cut(s) 385, 825
MluCI AATT 4 cut(s) 321, 575, 800, 1281
MluI ACGCGT 1 cut(s) 641
MlyI GAGTC 2 cut(s) 317, 416
MmeI TCCRAC 1 cut(s) 1213
Mph1103I ATGCAT 1 cut(s) 468
MroXI GAANNNNTTC 2 cut(s) 81, 736
MseI TTAA 4 cut(s) 398, 627, 690, 803
MslI CAYNNNNRTG 3 cut(s) 369, 471, 1152
MspA1I CMGCKG 1 cut(s) 911
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 1 cut(s) 89
MspR9I CCNGG 2 cut(s) 90, 1263
MvaI CCWGG 1 cut(s) 1263
MvnI CGCG 2 cut(s) 643, 705
MwoI GCNNNNNNNGC 6 cut(s) 146, 155, 219, 640, 771, 917
NciI CCSGG 1 cut(s) 90
NcoI CCATGG 1 cut(s) 241
NdeI CATATG 2 cut(s) 1140, 1308
NdeII GATC 5 cut(s) 180, 361, 1186, 1345, 1360
NlaIV GGNNCC 2 cut(s) 452, 1362
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 2 cut(s) 408, 757
NsiI ATGCAT 1 cut(s) 468
NspI RCATGY 1 cut(s) 688
OliI CACNNNNGTG 1 cut(s) 369
PasI CCCWGGG 1 cut(s) 1262
PcsI WCGNNNNNNNCGW 1 cut(s) 142
PdmI GAANNNNTTC 2 cut(s) 81, 736
PfeI GAWTC 2 cut(s) 868, 1320
PflFI GACNNNGTC 2 cut(s) 307, 755
PflMI CCANNNNNTGG 1 cut(s) 1116
PkrI GCNGC 5 cut(s) 253, 284, 656, 891, 1135
PleI GAGTC 2 cut(s) 316, 415
PpsI GAGTC 2 cut(s) 316, 415
PpuMI RGGWCCY 1 cut(s) 1247
Psp5II RGGWCCY 1 cut(s) 1247
Psp6I CCWGG 1 cut(s) 1261
PspGI CCWGG 1 cut(s) 1261
PspN4I GGNNCC 2 cut(s) 452, 1362
PspPI GGNCC 2 cut(s) 194, 1247
PspPPI RGGWCCY 1 cut(s) 1247
PstI CTGCAG 1 cut(s) 1342
PsuI RGATCY 1 cut(s) 1360
PsyI GACNNNGTC 2 cut(s) 307, 755
PvuII CAGCTG 1 cut(s) 911
RsaI GTAC 3 cut(s) 191, 717, 1212
RsaNI GTAC 3 cut(s) 190, 716, 1211
RseI CAYNNNNRTG 3 cut(s) 369, 471, 1152
SaqAI TTAA 4 cut(s) 398, 627, 690, 803
SatI GCNGC 5 cut(s) 252, 283, 655, 890, 1134
Sau3AI GATC 5 cut(s) 180, 361, 1186, 1345, 1360
Sau96I GGNCC 2 cut(s) 194, 1247
ScaI AGTACT 1 cut(s) 1212
SchI GAGTC 2 cut(s) 317, 416
ScrFI CCNGG 2 cut(s) 90, 1263
SduI GDGCHC 2 cut(s) 385, 825
SfaNI GCATC 1 cut(s) 783
SfcI CTRYAG 1 cut(s) 1338
SinI GGWCC 2 cut(s) 194, 1247
SmiMI CAYNNNNRTG 3 cut(s) 369, 471, 1152
SmlI CTYRAG 4 cut(s) 659, 689, 722, 1380
SmoI CTYRAG 4 cut(s) 659, 689, 722, 1380
Sse9I AATT 4 cut(s) 321, 575, 800, 1281
SsiI CCGC 3 cut(s) 86, 273, 889
SspMI CTAG 3 cut(s) 896, 951, 1191
StyD4I CCNGG 2 cut(s) 88, 1261
StyI CCWWGG 2 cut(s) 124, 241
TaaI ACNGT 4 cut(s) 194, 232, 493, 543
TaiI ACGT 1 cut(s) 881
TaqI TCGA 5 cut(s) 136, 866, 1086, 1252, 1318
TasI AATT 4 cut(s) 321, 575, 800, 1281
TatI WGTACW 1 cut(s) 1210
TauI GCSGC 1 cut(s) 892
TfiI GAWTC 2 cut(s) 868, 1320
Tru1I TTAA 4 cut(s) 398, 627, 690, 803
Tru9I TTAA 4 cut(s) 398, 627, 690, 803
TscAI CASTG 4 cut(s) 160, 417, 624, 1377
TseFI GTSAC 2 cut(s) 408, 757
TseI GCWGC 4 cut(s) 251, 282, 654, 1133
Tsp45I GTSAC 2 cut(s) 408, 757
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 4 cut(s) 160, 417, 624, 1377
Tth111I GACNNNGTC 2 cut(s) 307, 755
Van91I CCANNNNNTGG 1 cut(s) 1116
Vha464I CTTAAG 1 cut(s) 689
VneI GTGCAC 1 cut(s) 821
VpaK11BI GGWCC 2 cut(s) 194, 1247
XagI CCTNNNNNAGG 1 cut(s) 1380
XapI RAATTY 2 cut(s) 321, 575
XceI RCATGY 1 cut(s) 688
XmnI GAANNNNTTC 2 cut(s) 81, 736
XspI CTAG 3 cut(s) 896, 951, 1191
ZrmI AGTACT 1 cut(s) 1212
Zsp2I ATGCAT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.