Rmu_sc0000533.1_g000016

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000533.1
OG0000704
Physical Location & Seq
Forward (+)
45424 .. 47254
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000533.1_g000016.1.cds

Sequence Viewer

Length: 1200 bp
atgcgtgccaacactgcagaggattggcttgaacaaaggatcagaaagtatggtccagtttcaaagctaagtctctttggcaagccaacagtcttcatccatggacaagctgcaaacaagtttatattcaccagtgatgggagcatcatttcagcgcaacaaaccgagtctattcgtaggattttgggggaacggagtatatccgagttaagtgctgaggatcacaaacgtgtcagaagtgcacttatgctattcttgaagcctgagtcactgaagcaatatctatggaaaatggatgaagaaatcaggaaacaccttcagatgcactggcatggcaaacaacaagtaacagttatgcccctgatgaagaacctcacatttaacataatatcctctctactttttgggcttgaaagaggaactcggagagatgaatttttggagtgttttgaaaagatgatgcaaggattgtggtcagtgccggtcaacttgcccttcacgcgttacaacagcagcctcaaggcaagcaagagggttcaaaaaatgctcaaagaacttatacgcgaaaggagggtacaacttgatcaaaaaagggcttctccacaacaagacctcatcacttgcttccttagcatccggaatcatgaaggtaaagaagaattaacagagttggagatggtgcacaatgtcatggtagtcatggttgcagggcatgatacttcggctattcttattacgttccttttgcggattctagctaatgaaccagcagtgtatgctgcccttcatcaagagcaggaagaaatagctagaaccaaattggcaggagaatttttaacatgggaagatcttgccaaaatgaaatacacatggagtgtagcactggaaactctgagaatgattcctccttcctttggtagctttaggacagcaaagatattgagttcggtggattccacattcctaaagggtggcaggttcaacaaccaagcaacagttccaccctactgctttgtaccatttggagggggacctagaatatgtccaggatatgagtttgccaggatcgaaaccctaattgcaatgcaccatatggtaactcaattcacctggaagctatgtgcagacaatcacttcagtagggatctattgcctgtacctactcaaggactgcccattgaaatcatgccaagaatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

399

Amino Acids

45.99

Weight (kDa)

9.34

Isoelectric Point (pI)

57.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 966
AccII CGCG 2 cut(s) 502, 564
AccIII TCCGGA 1 cut(s) 636
AciI CCGC 1 cut(s) 748
AclWI GGATC 4 cut(s) 47, 228, 1073, 1152
AcsI RAATTY 2 cut(s) 434, 830
AcuI CTGAAG 3 cut(s) 293, 302, 1120
AfaI GTAC 3 cut(s) 576, 1017, 1158
AfiI CCNNNNNNNGG 4 cut(s) 138, 914, 1025, 1166
AflIII ACRYGT 2 cut(s) 229, 500
AgsI TTSAA 8 cut(s) 32, 63, 259, 413, 452, 539, 982, 1181
AjnI CCWGG 3 cut(s) 1045, 1061, 1109
AjuI GAANNNNNNNTTGG 2 cut(s) 981, 1013
AleI CACNNNNGTG 1 cut(s) 228
AluBI AGCT 6 cut(s) 67, 110, 758, 809, 921, 1117
AluI AGCT 6 cut(s) 67, 110, 758, 809, 921, 1117
Alw21I GWGCWC 2 cut(s) 244, 684
Alw26I GTCTC 1 cut(s) 77
Alw44I GTGCAC 2 cut(s) 240, 680
AlwI GGATC 4 cut(s) 47, 228, 1073, 1152
Aor13HI TCCGGA 1 cut(s) 636
ApaLI GTGCAC 2 cut(s) 240, 680
ApeKI GCWGC 3 cut(s) 110, 513, 779
ApoI RAATTY 2 cut(s) 434, 830
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 53, 1031
AsuHPI GGTGA 2 cut(s) 121, 1099
AvaII GGWCC 2 cut(s) 53, 1031
BaeGI GKGCMC 2 cut(s) 244, 684
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 2 cut(s) 244, 684
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 3 cut(s) 97, 525, 766
BccI CCATC 2 cut(s) 131, 670
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 3 cut(s) 1047, 1063, 1111
BclI TGATCA 1 cut(s) 583
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 3 cut(s) 755, 810, 1035
BfmI CTRYAG 1 cut(s) 15
BfuAI ACCTGC 1 cut(s) 966
BglII AGATCT 1 cut(s) 847
BisI GCNGC 3 cut(s) 111, 514, 780
BlsI GCNGC 3 cut(s) 112, 515, 781
Bme1390I CCNGG 3 cut(s) 1047, 1063, 1111
Bme18I GGWCC 2 cut(s) 53, 1031
BmgT120I GGNCC 2 cut(s) 53, 1031
BmiI GGNNCC 1 cut(s) 1032
BmrFI CCNGG 3 cut(s) 1047, 1063, 1111
BmsI GCATC 4 cut(s) 153, 312, 450, 642
BpiI GAAGAC 1 cut(s) 85
Bpu10I CCTNAGC 2 cut(s) 216, 629
BpuEI CTTGAG 2 cut(s) 503, 1149
BsaJI CCNNGG 1 cut(s) 100
BsaWI WCCGGW 1 cut(s) 636
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 914, 1025, 1166
Bse118I RCCGGY 1 cut(s) 481
Bse1I ACTGG 4 cut(s) 56, 132, 332, 888
Bse3DI GCAATG 1 cut(s) 1089
BseAI TCCGGA 1 cut(s) 636
BseBI CCWGG 3 cut(s) 1047, 1063, 1111
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 3 cut(s) 96, 301, 633
BseLI CCNNNNNNNGG 4 cut(s) 138, 914, 1025, 1166
BseMI GCAATG 1 cut(s) 1089
BseMII CTCAG 3 cut(s) 207, 255, 884
BseNI ACTGG 4 cut(s) 56, 132, 332, 888
BseSI GKGCMC 2 cut(s) 244, 684
BseXI GCAGC 3 cut(s) 97, 525, 766
BsgI GTGCAG 1 cut(s) 1143
Bsh1236I CGCG 2 cut(s) 502, 564
BsiHKAI GWGCWC 2 cut(s) 244, 684
BsiSI CCGG 2 cut(s) 482, 637
BslFI GGGAC 1 cut(s) 1044
BslI CCNNNNNNNGG 4 cut(s) 138, 914, 1025, 1166
BsmAI GTCTC 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 1044
Bsp1286I GDGCHC 2 cut(s) 244, 684
Bsp13I TCCGGA 1 cut(s) 636
Bsp143I GATC 6 cut(s) 39, 220, 583, 847, 1065, 1144
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 1 cut(s) 748
BspCNI CTCAG 3 cut(s) 208, 256, 885
BspEI TCCGGA 1 cut(s) 636
BspFNI CGCG 2 cut(s) 502, 564
BspHI TCATGA 1 cut(s) 643
BspLI GGNNCC 1 cut(s) 1032
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 966
BspPI GGATC 4 cut(s) 47, 228, 1073, 1152
BsrDI GCAATG 1 cut(s) 1089
BsrFI RCCGGY 1 cut(s) 481
BsrI ACTGG 4 cut(s) 56, 132, 332, 888
BssAI RCCGGY 1 cut(s) 481
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 6 cut(s) 39, 220, 583, 847, 1065, 1144
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 3 cut(s) 1047, 1063, 1111
Bst4CI ACNGT 3 cut(s) 91, 352, 997
BstAPI GCANNNNNTGC 1 cut(s) 776
BstC8I GCNNGC 3 cut(s) 6, 83, 526
BstDEI CTNAG 5 cut(s) 68, 216, 264, 629, 893
BstDSI CCRYGG 1 cut(s) 100
BstENI CCTNNNNNAGG 1 cut(s) 1164
BstF5I GGATG 3 cut(s) 96, 301, 633
BstFNI CGCG 2 cut(s) 502, 564
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 6 cut(s) 42, 223, 586, 850, 1068, 1147
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 6 cut(s) 39, 220, 583, 847, 1065, 1144
BstMWI GCNNNNNNNGC 4 cut(s) 14, 499, 630, 776
BstNI CCWGG 3 cut(s) 1047, 1063, 1111
BstSCI CCNGG 3 cut(s) 1045, 1061, 1109
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 2 cut(s) 244, 684
BstUI CGCG 2 cut(s) 502, 564
BstV1I GCAGC 3 cut(s) 97, 525, 766
BstV2I GAAGAC 1 cut(s) 85
BstX2I RGATCY 2 cut(s) 847, 1144
BstYI RGATCY 2 cut(s) 847, 1144
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 3 cut(s) 96, 301, 633
BtsI GCAGTG 2 cut(s) 12, 777
BtsIMutI CAGTG 7 cut(s) 12, 139, 269, 325, 483, 777, 881
BveI ACCTGC 1 cut(s) 966
Cac8I GCNNGC 3 cut(s) 6, 83, 526
CciI TCATGA 1 cut(s) 643
CfoI GCGC 1 cut(s) 157
Cfr10I RCCGGY 1 cut(s) 481
Cfr13I GGNCC 2 cut(s) 53, 1031
Csp6I GTAC 3 cut(s) 575, 1016, 1157
CspCI CAANNNNNGTGG 2 cut(s) 452, 487
CviAII CATG 9 cut(s) 101, 332, 644, 691, 700, 713, 840, 870, 1186
CviQI GTAC 3 cut(s) 575, 1016, 1157
DdeI CTNAG 5 cut(s) 68, 216, 264, 629, 893
DpnI GATC 6 cut(s) 41, 222, 585, 849, 1067, 1146
DpnII GATC 6 cut(s) 39, 220, 583, 847, 1065, 1144
Eco130I CCWWGG 1 cut(s) 100
Eco47I GGWCC 2 cut(s) 53, 1031
Eco57I CTGAAG 3 cut(s) 293, 302, 1120
EcoNI CCTNNNNNAGG 1 cut(s) 1164
EcoO109I RGGNCCY 1 cut(s) 1031
EcoRII CCWGG 3 cut(s) 1045, 1061, 1109
EcoT14I CCWWGG 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 9 cut(s) 104, 335, 647, 694, 703, 716, 843, 873, 1189
FaqI GGGAC 1 cut(s) 1044
FatI CATG 9 cut(s) 100, 331, 643, 690, 699, 712, 839, 869, 1185
FauNDI CATATG 1 cut(s) 1092
FbaI TGATCA 1 cut(s) 583
Fnu4HI GCNGC 3 cut(s) 111, 514, 780
FokI GGATG 3 cut(s) 83, 308, 620
Fsp4HI GCNGC 3 cut(s) 111, 514, 780
FspBI CTAG 3 cut(s) 755, 810, 1035
GlaI GCGC 1 cut(s) 156
GluI GCNGC 3 cut(s) 111, 514, 780
HapII CCGG 2 cut(s) 482, 637
HhaI GCGC 1 cut(s) 157
Hin1II CATG 9 cut(s) 104, 335, 647, 694, 703, 716, 843, 873, 1189
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HinfI GANTC 6 cut(s) 167, 266, 640, 751, 901, 953
HpaII CCGG 2 cut(s) 482, 637
HphI GGTGA 2 cut(s) 121, 1099
Hpy166II GTNNAC 3 cut(s) 242, 487, 682
Hpy188I TCNGA 6 cut(s) 44, 205, 236, 321, 426, 894
Hpy188III TCNNGA 5 cut(s) 256, 307, 637, 644, 791
Hpy8I GTNNAC 3 cut(s) 242, 487, 682
HpyAV CCTTC 5 cut(s) 326, 505, 641, 794, 918
HpyCH4III ACNGT 3 cut(s) 91, 352, 997
HpyCH4IV ACGT 2 cut(s) 229, 737
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 499, 630, 776
HpyF3I CTNAG 5 cut(s) 68, 216, 264, 629, 893
HpySE526I ACGT 2 cut(s) 229, 737
Hsp92II CATG 9 cut(s) 104, 335, 647, 694, 703, 716, 843, 873, 1189
HspAI GCGC 1 cut(s) 155
Kpn2I TCCGGA 1 cut(s) 636
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 6 cut(s) 39, 220, 583, 847, 1065, 1144
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 3 cut(s) 97, 525, 766
LweI GCATC 4 cut(s) 153, 312, 450, 642
MaeI CTAG 3 cut(s) 755, 810, 1035
MaeII ACGT 2 cut(s) 229, 737
MaeIII GTNAC 4 cut(s) 267, 346, 503, 1096
MalI GATC 6 cut(s) 41, 222, 585, 849, 1067, 1146
MboI GATC 6 cut(s) 39, 220, 583, 847, 1065, 1144
MboII GAAGA 6 cut(s) 85, 311, 379, 668, 812, 857
MflI RGATCY 2 cut(s) 847, 1144
MhlI GDGCHC 2 cut(s) 244, 684
MluCI AATT 6 cut(s) 434, 659, 818, 830, 1077, 1103
MluI ACGCGT 1 cut(s) 500
MlyI GAGTC 2 cut(s) 176, 275
MmeI TCCRAC 1 cut(s) 651
MroI TCCGGA 1 cut(s) 636
MseI TTAA 4 cut(s) 209, 381, 662, 836
MslI CAYNNNNRTG 2 cut(s) 228, 330
MspI CCGG 2 cut(s) 482, 637
MspR9I CCNGG 3 cut(s) 1047, 1063, 1111
MvaI CCWGG 3 cut(s) 1047, 1063, 1111
MvnI CGCG 2 cut(s) 502, 564
MwoI GCNNNNNNNGC 4 cut(s) 14, 499, 630, 776
NcoI CCATGG 1 cut(s) 100
NdeI CATATG 1 cut(s) 1092
NdeII GATC 6 cut(s) 39, 220, 583, 847, 1065, 1144
NlaIII CATG 9 cut(s) 104, 335, 647, 694, 703, 716, 843, 873, 1189
NlaIV GGNNCC 1 cut(s) 1032
NmuCI GTSAC 1 cut(s) 267
OliI CACNNNNGTG 1 cut(s) 228
PagI TCATGA 1 cut(s) 643
PfeI GAWTC 4 cut(s) 640, 751, 901, 953
PfoI TCCNGGA 1 cut(s) 1045
PkrI GCNGC 3 cut(s) 112, 515, 781
PleI GAGTC 2 cut(s) 175, 274
PpsI GAGTC 2 cut(s) 175, 274
PpuMI RGGWCCY 1 cut(s) 1031
Psp5II RGGWCCY 1 cut(s) 1031
Psp6I CCWGG 3 cut(s) 1045, 1061, 1109
PspGI CCWGG 3 cut(s) 1045, 1061, 1109
PspN4I GGNNCC 1 cut(s) 1032
PspPI GGNCC 2 cut(s) 53, 1031
PspPPI RGGWCCY 1 cut(s) 1031
PstI CTGCAG 1 cut(s) 19
PsuI RGATCY 2 cut(s) 847, 1144
RsaI GTAC 3 cut(s) 576, 1017, 1158
RsaNI GTAC 3 cut(s) 575, 1016, 1157
RseI CAYNNNNRTG 2 cut(s) 228, 330
SaqAI TTAA 4 cut(s) 209, 381, 662, 836
SatI GCNGC 3 cut(s) 111, 514, 780
Sau3AI GATC 6 cut(s) 39, 220, 583, 847, 1065, 1144
Sau96I GGNCC 2 cut(s) 53, 1031
SchI GAGTC 2 cut(s) 176, 275
ScrFI CCNGG 3 cut(s) 1047, 1063, 1111
SduI GDGCHC 2 cut(s) 244, 684
SfaNI GCATC 4 cut(s) 153, 312, 450, 642
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 2 cut(s) 53, 1031
SmiMI CAYNNNNRTG 2 cut(s) 228, 330
SmlI CTYRAG 2 cut(s) 518, 1164
SmoI CTYRAG 2 cut(s) 518, 1164
Sse9I AATT 6 cut(s) 434, 659, 818, 830, 1077, 1103
SsiI CCGC 1 cut(s) 748
SspMI CTAG 3 cut(s) 755, 810, 1035
StyD4I CCNGG 3 cut(s) 1045, 1061, 1109
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 3 cut(s) 91, 352, 997
TaiI ACGT 2 cut(s) 232, 740
TaqI TCGA 1 cut(s) 1068
TasI AATT 6 cut(s) 434, 659, 818, 830, 1077, 1103
TfiI GAWTC 4 cut(s) 640, 751, 901, 953
Tru1I TTAA 4 cut(s) 209, 381, 662, 836
Tru9I TTAA 4 cut(s) 209, 381, 662, 836
TscAI CASTG 7 cut(s) 19, 139, 276, 332, 483, 777, 888
TseFI GTSAC 1 cut(s) 267
TseI GCWGC 3 cut(s) 110, 513, 779
Tsp45I GTSAC 1 cut(s) 267
TspDTI ATGAA 8 cut(s) 85, 312, 380, 447, 660, 776, 777, 875
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 7 cut(s) 19, 139, 276, 332, 483, 777, 888
VneI GTGCAC 2 cut(s) 240, 680
VpaK11BI GGWCC 2 cut(s) 53, 1031
XagI CCTNNNNNAGG 1 cut(s) 1164
XapI RAATTY 2 cut(s) 434, 830
XspI CTAG 3 cut(s) 755, 810, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.