Rh5BG268100

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000704
Physical Location & Seq
Reverse (-)
33484494 .. 33485671
1178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG268100.1

Sequence Viewer

Length: 531 bp
ATGGTGCACAATGTCATGGTAGTCATGGTTGCAGGACATGACACTTCATCTGTTCTGATTACGTTCCTTTTGCGGCTTCTCACTAATGAACCTGCTGTTTATGCAGCCTTCTTCAAGTTTCTAACATGGGAAGACCTTGCCAAAATGAAATACACATGGAAAGTAGTACTGGAAACTCTGAGGATGACTCCTCCTGTCTTTGGTGGCTTTAGGACTGCTGTCAAAGATATATTCTGGGCTGCTCCTATGACCCGCATGGATGATAACATATTCCCGGAGCCATCGAAGTTTGATCCAAGTAGGTTCGACAAGCAAGCAACAGTTCCACCCTACTGCTTTGTACCATTTGGAGGGGGACCTAGAATATGTCCAGGATATGAGTTTGCCAGGATCGAAACCCTAATTGCAATACACCATATGGTAACTCAATTCACTTGGAAGCTATGTGCAGACAATCACTTCAGTAGGGATCTAATGCCTGTACCTACTCAAGGACTGCCCATTGAAATCACGCCTAGAATACTACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

176

Amino Acids

20.14

Weight (kDa)

7.76

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 153 3.8e-29 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 100
AciI CCGC 2 cut(s) 73, 253
AclWI GGATC 3 cut(s) 287, 398, 477
AcuI CTGAAG 1 cut(s) 445
AfaI GTAC 3 cut(s) 168, 342, 483
AfiI CCNNNNNNNGG 3 cut(s) 200, 350, 491
AgsI TTSAA 2 cut(s) 115, 506
AjnI CCWGG 2 cut(s) 370, 386
AluBI AGCT 1 cut(s) 442
AluI AGCT 1 cut(s) 442
Alw21I GWGCWC 1 cut(s) 9
Alw44I GTGCAC 1 cut(s) 5
AlwI GGATC 3 cut(s) 287, 398, 477
ApaLI GTGCAC 1 cut(s) 5
ApeKI GCWGC 2 cut(s) 104, 239
AspS9I GGNCC 1 cut(s) 356
AsuC2I CCSGG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 356
BaeGI GKGCMC 1 cut(s) 9
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 116, 226
BccI CCATC 1 cut(s) 289
BciT130I CCWGG 2 cut(s) 372, 388
BcnI CCSGG 1 cut(s) 275
BfaI CTAG 2 cut(s) 360, 516
BfuAI ACCTGC 1 cut(s) 100
BisI GCNGC 3 cut(s) 74, 105, 240
BlsI GCNGC 3 cut(s) 75, 106, 241
BmcAI AGTACT 1 cut(s) 168
Bme1390I CCNGG 3 cut(s) 275, 372, 388
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 2 cut(s) 279, 357
BmrFI CCNGG 3 cut(s) 275, 372, 388
BoxI GACNNNNGTC 1 cut(s) 218
BpiI GAAGAC 1 cut(s) 138
BplI GAGNNNNNCTC 2 cut(s) 172, 204
BpuEI CTTGAG 1 cut(s) 474
BpuMI CCSGG 1 cut(s) 275
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 350, 491
Bse1I ACTGG 1 cut(s) 174
BseBI CCWGG 2 cut(s) 372, 388
BseGI GGATG 2 cut(s) 189, 265
BseLI CCNNNNNNNGG 3 cut(s) 200, 350, 491
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 1 cut(s) 174
BseRI GAGGAG 1 cut(s) 180
BseSI GKGCMC 1 cut(s) 9
BseXI GCAGC 2 cut(s) 116, 226
BsgI GTGCAG 1 cut(s) 468
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 3 cut(s) 200, 350, 491
BsmFI GGGAC 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 3 cut(s) 292, 390, 469
BspACI CCGC 2 cut(s) 73, 253
BspCNI CTCAG 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 279, 357
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 3 cut(s) 287, 398, 477
BsrI ACTGG 1 cut(s) 174
BssMI GATC 3 cut(s) 292, 390, 469
Bst2UI CCWGG 2 cut(s) 372, 388
Bst4CI ACNGT 2 cut(s) 322, 528
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 1 cut(s) 179
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 2 cut(s) 189, 265
BstKTI GATC 3 cut(s) 295, 393, 472
BstMBI GATC 3 cut(s) 292, 390, 469
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstNI CCWGG 2 cut(s) 372, 388
BstPAI GACNNNNGTC 1 cut(s) 218
BstSCI CCNGG 3 cut(s) 273, 370, 386
BstSLI GKGCMC 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 116, 226
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BtsCI GGATG 2 cut(s) 189, 265
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 356
Csp6I GTAC 3 cut(s) 167, 341, 482
CviAII CATG 6 cut(s) 16, 25, 38, 126, 156, 256
CviJI RGCY 6 cut(s) 76, 107, 207, 239, 280, 442
CviKI_1 RGCY 6 cut(s) 76, 107, 207, 239, 280, 442
CviQI GTAC 3 cut(s) 167, 341, 482
DdeI CTNAG 1 cut(s) 179
DpnI GATC 3 cut(s) 294, 392, 471
DpnII GATC 3 cut(s) 292, 390, 469
Eco47I GGWCC 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 445
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 370, 386
FaeI CATG 6 cut(s) 19, 28, 41, 129, 159, 259
FaqI GGGAC 1 cut(s) 369
FatI CATG 6 cut(s) 15, 24, 37, 125, 155, 255
FauI CCCGC 1 cut(s) 260
FauNDI CATATG 1 cut(s) 417
Fnu4HI GCNGC 3 cut(s) 74, 105, 240
FokI GGATG 2 cut(s) 196, 272
Fsp4HI GCNGC 3 cut(s) 74, 105, 240
FspBI CTAG 2 cut(s) 360, 516
GluI GCNGC 3 cut(s) 74, 105, 240
HapII CCGG 1 cut(s) 275
Hin1II CATG 6 cut(s) 19, 28, 41, 129, 159, 259
HinfI GANTC 1 cut(s) 187
HpaII CCGG 1 cut(s) 275
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 2 cut(s) 57, 180
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 2 cut(s) 322, 528
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 5 cut(s) 7, 32, 104, 407, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpyF3I CTNAG 1 cut(s) 179
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 6 cut(s) 19, 28, 41, 129, 159, 259
Kzo9I GATC 3 cut(s) 292, 390, 469
LmnI GCTCC 2 cut(s) 247, 277
Lsp1109I GCAGC 2 cut(s) 116, 226
MaeI CTAG 2 cut(s) 360, 516
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 421
MalI GATC 3 cut(s) 294, 392, 471
MboI GATC 3 cut(s) 292, 390, 469
MboII GAAGA 2 cut(s) 103, 143
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 2 cut(s) 402, 428
MlyI GAGTC 1 cut(s) 181
MnlI CCTC 3 cut(s) 174, 201, 344
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 3 cut(s) 275, 372, 388
MvaI CCWGG 2 cut(s) 372, 388
MwoI GCNNNNNNNGC 1 cut(s) 101
NciI CCSGG 1 cut(s) 275
NdeI CATATG 1 cut(s) 417
NdeII GATC 3 cut(s) 292, 390, 469
NlaIII CATG 6 cut(s) 19, 28, 41, 129, 159, 259
NlaIV GGNNCC 2 cut(s) 279, 357
PfoI TCCNGGA 2 cut(s) 273, 370
PkrI GCNGC 3 cut(s) 75, 106, 241
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PpuMI RGGWCCY 1 cut(s) 356
PshAI GACNNNNGTC 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 356
Psp6I CCWGG 2 cut(s) 370, 386
PspGI CCWGG 2 cut(s) 370, 386
PspN4I GGNNCC 2 cut(s) 279, 357
PspPI GGNCC 1 cut(s) 356
PspPPI RGGWCCY 1 cut(s) 356
PsuI RGATCY 1 cut(s) 469
RsaI GTAC 3 cut(s) 168, 342, 483
RsaNI GTAC 3 cut(s) 167, 341, 482
SatI GCNGC 3 cut(s) 74, 105, 240
Sau3AI GATC 3 cut(s) 292, 390, 469
Sau96I GGNCC 1 cut(s) 356
ScaI AGTACT 1 cut(s) 168
SchI GAGTC 1 cut(s) 181
ScrFI CCNGG 3 cut(s) 275, 372, 388
SduI GDGCHC 1 cut(s) 9
SetI ASST 7 cut(s) 65, 94, 138, 305, 361, 444, 487
SinI GGWCC 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 489
SmoI CTYRAG 1 cut(s) 489
Sse9I AATT 2 cut(s) 402, 428
SsiI CCGC 2 cut(s) 73, 253
SspMI CTAG 2 cut(s) 360, 516
StyD4I CCNGG 3 cut(s) 273, 370, 386
TaaI ACNGT 2 cut(s) 322, 528
TaiI ACGT 1 cut(s) 65
TaqI TCGA 3 cut(s) 284, 306, 393
TasI AATT 2 cut(s) 402, 428
TatI WGTACW 1 cut(s) 166
TauI GCSGC 1 cut(s) 76
TseI GCWGC 2 cut(s) 104, 239
TspDTI ATGAA 3 cut(s) 36, 102, 161
VneI GTGCAC 1 cut(s) 5
VpaK11BI GGWCC 1 cut(s) 356
XagI CCTNNNNNAGG 1 cut(s) 489
XspI CTAG 2 cut(s) 360, 516
ZrmI AGTACT 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.