FvH4_4g02612

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001250
Physical Location & Seq
Reverse (-)
2375611 .. 2378108
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02612.t1

Sequence Viewer

Length: 1212 bp
ATGTCATCACCAAGATCCTCCTCCTCGTCGCCATCATCCACGCCACTGACCATTACCAGTGCTTCCCTTTCATCCTCGATGACAAACACCCCAACTCCCTTAGCAACTCCCCTCCAGACGGGACTGTCAGCATCCTCATCTGAGCCGACAGTAGTTGCACCGGAGACGTTTGATATCGATAAAATCATGGACGAATTTCCACAGGCCGAATTAGTAGACTACCATGAGACACAGAGAGGCCAAGTAGTCTCTCCATCATCGTCGAAAGCATTGGTTTTATATAAACCGAAGGCACCACGCCTCGAAAACCTGCAAGAACGACTTGAAGATGTCGTCAGGGACCTTGTTCTTGGAGCGGCAAAAGAGTTGAATGACCTAGCAGATGCAGAGATAAATATGGAACAAAAGCAGGAACAGGCCTCTGACTCAACCAAGGAAGAAGGTGAACTGATGATAGCAACACCACAACAAGCTCCGACTCACCATGAGAGCCCTAAGAGAACTGAGACGAAACCGGCCGTCACCAGACGCCAAGCTCAATCGGCCACCAAGCAGGTCGCCCATATCGCCAATGTGTCATTCGAAATTCCGGATGAGTTTAAGGTAGACGATGACACAATAAAAAAGCTTGTGCCTATGGATGAAGCCCTCAGGCACATTACTGCTCAGAGTGGGAAAAAGTATAGTTTCTACGACAGACTCCATGGATGGAAAATGGAGTGTAGAATAAGACTGAATGGCTCGCGACAATATGATAAGTATTATACACATTTACAATCCAAGACTGTGACGAGATCAGTACCTGAAGCTGTTAGGTATATTCTTTACGAGGTGACTCCTCAGCAAATGAGAGAAGCCAAGAAAAAACGAAAAATGGAGATTCCTGATGAGACTAGTTCTCAAAATGTATTAATCGATGAAGTCCTAAACATGTCACCGGAAGATATTGCTGCCGTTTTAGAAGTAGATGGGGAGACATACGTTGAAATGTGGTATCGCATAGCTTGGACTAACTTAATTAACAGCACTGCAGTGAAGACGCCATCAGAAGCAAAATGGGAAGAACTGAAGGCTGCAAAACAGACGGCCGAAACCACAAGAAGCAAACAAGGGGATTGTACACCAACATTAGATGTTGAAGCTGCGAACGCATTGATCGGACTAGCTGCGGAAGAAAGTTCGGGAATAAAAAGGCAGAAGACTTCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

404

Amino Acids

44.91

Weight (kDa)

5.35

Isoelectric Point (pI)

59.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 124
Acc36I ACCTGC 2 cut(s) 318, 544
AccB1I GGYRCC 1 cut(s) 292
AccBSI CCGCTC 1 cut(s) 356
AccI GTMKAC 2 cut(s) 216, 606
AccII CGCG 1 cut(s) 745
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 2 cut(s) 356, 1169
AclWI GGATC 1 cut(s) 9
AcoI YGGCCR 3 cut(s) 516, 543, 1086
AcsI RAATTY 2 cut(s) 194, 585
AcuI CTGAAG 2 cut(s) 825, 1088
AcyI GRCGYC 2 cut(s) 529, 1040
AfaI GTAC 2 cut(s) 801, 1120
AfiI CCNNNNNNNGG 1 cut(s) 118
AflIII ACRYGT 1 cut(s) 930
AgsI TTSAA 4 cut(s) 326, 370, 986, 1139
AhlI ACTAGT 1 cut(s) 893
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AleI CACNNNNGTG 1 cut(s) 1031
AluBI AGCT 7 cut(s) 473, 536, 628, 809, 1004, 1142, 1166
AluI AGCT 7 cut(s) 473, 536, 628, 809, 1004, 1142, 1166
Alw26I GTCTC 6 cut(s) 158, 221, 253, 500, 884, 968
AlwI GGATC 1 cut(s) 9
AlwNI CAGNNNCTG 1 cut(s) 803
Aor13HI TCCGGA 1 cut(s) 589
AoxI GGCC 6 cut(s) 204, 238, 417, 516, 543, 1086
ApeKI GCWGC 4 cut(s) 950, 1073, 1142, 1166
ApoI RAATTY 2 cut(s) 194, 585
AseI ATTAAT 1 cut(s) 911
AspS9I GGNCC 1 cut(s) 340
AsuHPI GGTGA 5 cut(s) 455, 473, 514, 844, 927
AsuII TTCGAA 1 cut(s) 582
AvaII GGWCC 1 cut(s) 340
AxyI CCTNAGG 1 cut(s) 650
BanI GGYRCC 1 cut(s) 292
BanII GRGCYC 1 cut(s) 494
BbsI GAAGAC 2 cut(s) 1043, 1205
BbvCI CCTCAGC 1 cut(s) 840
BbvI GCAGC 4 cut(s) 937, 1060, 1129, 1153
BccI CCATC 5 cut(s) 40, 262, 702, 962, 1051
BceAI ACGGC 3 cut(s) 503, 938, 1101
BcoDI GTCTC 6 cut(s) 158, 221, 253, 500, 884, 968
BcuI ACTAGT 1 cut(s) 893
BfaI CTAG 3 cut(s) 377, 894, 1163
BfmI CTRYAG 1 cut(s) 1029
BfuAI ACCTGC 2 cut(s) 318, 544
BisI GCNGC 5 cut(s) 357, 951, 1074, 1143, 1167
BlsI GCNGC 5 cut(s) 358, 952, 1075, 1144, 1168
Bme18I GGWCC 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 340
BmiI GGNNCC 2 cut(s) 294, 341
BmsI GCATC 2 cut(s) 140, 373
BpiI GAAGAC 2 cut(s) 1043, 1205
BpmI CTGGAG 1 cut(s) 98
Bpu10I CCTNAGC 2 cut(s) 100, 840
Bpu14I TTCGAA 1 cut(s) 582
Bsa29I ATCGAT 2 cut(s) 177, 915
BsaHI GRCGYC 2 cut(s) 529, 1040
BsaJI CCNNGG 2 cut(s) 432, 703
BsaWI WCCGGW 3 cut(s) 160, 589, 937
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse118I RCCGGY 1 cut(s) 514
Bse1I ACTGG 1 cut(s) 57
Bse21I CCTNAGG 1 cut(s) 650
BseAI TCCGGA 1 cut(s) 589
BseCI ATCGAT 2 cut(s) 177, 915
BseDI CCNNGG 2 cut(s) 432, 703
BseGI GGATG 6 cut(s) 35, 71, 131, 598, 646, 713
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 5 cut(s) 132, 495, 664, 680, 854
BseNI ACTGG 1 cut(s) 57
BseRI GAGGAG 3 cut(s) 10, 13, 828
BseX3I CGGCCG 2 cut(s) 516, 1086
BseXI GCAGC 4 cut(s) 937, 1060, 1129, 1153
Bsh1236I CGCG 1 cut(s) 745
Bsh1285I CGRYCG 2 cut(s) 519, 1089
BshFI GGCC 6 cut(s) 206, 240, 419, 518, 545, 1088
BshNI GGYRCC 1 cut(s) 292
BshVI ATCGAT 2 cut(s) 177, 915
BsiEI CGRYCG 2 cut(s) 519, 1089
BsiSI CCGG 4 cut(s) 161, 515, 590, 938
BslFI GGGAC 2 cut(s) 135, 353
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 6 cut(s) 158, 221, 253, 500, 884, 968
BsmBI CGTCTC 2 cut(s) 158, 500
BsmFI GGGAC 2 cut(s) 135, 353
BsnI GGCC 6 cut(s) 206, 240, 419, 518, 545, 1088
Bsp119I TTCGAA 1 cut(s) 582
Bsp1286I GDGCHC 1 cut(s) 494
Bsp13I TCCGGA 1 cut(s) 589
Bsp1407I TGTACA 1 cut(s) 1118
Bsp143I GATC 3 cut(s) 14, 794, 1155
Bsp19I CCATGG 1 cut(s) 703
Bsp68I TCGCGA 1 cut(s) 745
BspACI CCGC 2 cut(s) 356, 1169
BspANI GGCC 6 cut(s) 206, 240, 419, 518, 545, 1088
BspCNI CTCAG 5 cut(s) 133, 496, 663, 679, 853
BspDI ATCGAT 2 cut(s) 177, 915
BspEI TCCGGA 1 cut(s) 589
BspFNI CGCG 1 cut(s) 745
BspLI GGNNCC 2 cut(s) 294, 341
BspMAI CTGCAG 1 cut(s) 1033
BspMI ACCTGC 2 cut(s) 318, 544
BspPI GGATC 1 cut(s) 9
BspT104I TTCGAA 1 cut(s) 582
BspT107I GGYRCC 1 cut(s) 292
BsrBI CCGCTC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 514
BsrGI TGTACA 1 cut(s) 1118
BsrI ACTGG 1 cut(s) 57
BssAI RCCGGY 1 cut(s) 514
BssECI CCNNGG 2 cut(s) 432, 703
BssMI GATC 3 cut(s) 14, 794, 1155
BssNI GRCGYC 2 cut(s) 529, 1040
BssT1I CCWWGG 2 cut(s) 432, 703
Bst4CI ACNGT 3 cut(s) 126, 151, 787
BstACI GRCGYC 2 cut(s) 529, 1040
BstAUI TGTACA 1 cut(s) 1118
BstBI TTCGAA 1 cut(s) 582
BstC8I GCNNGC 1 cut(s) 743
BstDEI CTNAG 7 cut(s) 100, 141, 495, 504, 650, 666, 840
BstDSI CCRYGG 1 cut(s) 703
BstF5I GGATG 6 cut(s) 35, 71, 131, 598, 646, 713
BstFNI CGCG 1 cut(s) 745
BstKTI GATC 3 cut(s) 17, 797, 1158
BstMAI GTCTC 6 cut(s) 158, 221, 253, 500, 884, 968
BstMBI GATC 3 cut(s) 14, 794, 1155
BstMCI CGRYCG 2 cut(s) 519, 1089
BstMWI GCNNNNNNNGC 3 cut(s) 542, 566, 1148
BstNSI RCATGY 1 cut(s) 934
BstSFI CTRYAG 1 cut(s) 1029
BstUI CGCG 1 cut(s) 745
BstV1I GCAGC 4 cut(s) 937, 1060, 1129, 1153
BstV2I GAAGAC 2 cut(s) 1043, 1205
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
BstZI CGGCCG 2 cut(s) 516, 1086
Bsu15I ATCGAT 2 cut(s) 177, 915
Bsu36I CCTNAGG 1 cut(s) 650
BsuRI GGCC 6 cut(s) 206, 240, 419, 518, 545, 1088
BsuTUI ATCGAT 2 cut(s) 177, 915
BtgI CCRYGG 1 cut(s) 703
BtsCI GGATG 6 cut(s) 35, 71, 131, 598, 646, 713
BtsI GCAGTG 2 cut(s) 1026, 1038
BtsIMutI CAGTG 4 cut(s) 44, 64, 1026, 1038
BtuMI TCGCGA 1 cut(s) 745
BveI ACCTGC 2 cut(s) 318, 544
Cac8I GCNNGC 1 cut(s) 743
CaiI CAGNNNCTG 1 cut(s) 803
Cfr10I RCCGGY 1 cut(s) 514
Cfr13I GGNCC 1 cut(s) 340
ClaI ATCGAT 2 cut(s) 177, 915
CseI GACGC 2 cut(s) 537, 1048
Csp6I GTAC 2 cut(s) 800, 1119
CviAII CATG 5 cut(s) 187, 224, 485, 704, 931
CviQI GTAC 2 cut(s) 800, 1119
DdeI CTNAG 7 cut(s) 100, 141, 495, 504, 650, 666, 840
DpnI GATC 3 cut(s) 16, 796, 1157
DpnII GATC 3 cut(s) 14, 794, 1155
DrdI GACNNNNNNGTC 1 cut(s) 124
DseDI GACNNNNNNGTC 1 cut(s) 124
EaeI YGGCCR 3 cut(s) 516, 543, 1086
EagI CGGCCG 2 cut(s) 516, 1086
EclXI CGGCCG 2 cut(s) 516, 1086
Eco130I CCWWGG 2 cut(s) 432, 703
Eco147I AGGCCT 1 cut(s) 419
Eco24I GRGCYC 1 cut(s) 494
Eco32I GATATC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 340
Eco52I CGGCCG 2 cut(s) 516, 1086
Eco57I CTGAAG 2 cut(s) 825, 1088
Eco81I CCTNAGG 1 cut(s) 650
EcoO109I RGGNCCY 1 cut(s) 340
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 2 cut(s) 432, 703
EcoT38I GRGCYC 1 cut(s) 494
ErhI CCWWGG 2 cut(s) 432, 703
Esp3I CGTCTC 2 cut(s) 158, 500
FaeI CATG 5 cut(s) 190, 227, 488, 707, 934
FalI AAGNNNNNCTT 2 cut(s) 306, 338
FaqI GGGAC 2 cut(s) 135, 353
FatI CATG 5 cut(s) 186, 223, 484, 703, 930
FblI GTMKAC 2 cut(s) 216, 606
Fnu4HI GCNGC 5 cut(s) 357, 951, 1074, 1143, 1167
FokI GGATG 6 cut(s) 22, 58, 118, 605, 653, 720
FriOI GRGCYC 1 cut(s) 494
Fsp4HI GCNGC 5 cut(s) 357, 951, 1074, 1143, 1167
FspBI CTAG 3 cut(s) 377, 894, 1163
GluI GCNGC 5 cut(s) 357, 951, 1074, 1143, 1167
GsuI CTGGAG 1 cut(s) 98
HaeIII GGCC 6 cut(s) 206, 240, 419, 518, 545, 1088
HapII CCGG 4 cut(s) 161, 515, 590, 938
HgaI GACGC 2 cut(s) 537, 1048
Hin1I GRCGYC 2 cut(s) 529, 1040
Hin1II CATG 5 cut(s) 190, 227, 488, 707, 934
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 5 cut(s) 425, 478, 699, 835, 880
HpaII CCGG 4 cut(s) 161, 515, 590, 938
HphI GGTGA 5 cut(s) 455, 473, 514, 844, 927
Hpy166II GTNNAC 4 cut(s) 217, 446, 607, 1121
Hpy188I TCNGA 6 cut(s) 142, 424, 477, 669, 1048, 1160
Hpy188III TCNNGA 5 cut(s) 115, 590, 744, 884, 1182
Hpy8I GTNNAC 4 cut(s) 217, 446, 607, 1121
Hpy99I CGWCG 2 cut(s) 31, 265
HpyAV CCTTC 3 cut(s) 283, 434, 1063
HpyCH4III ACNGT 3 cut(s) 126, 151, 787
HpyCH4IV ACGT 2 cut(s) 167, 981
HpyCH4V TGCA 5 cut(s) 158, 313, 386, 1031, 1076
HpyF10VI GCNNNNNNNGC 3 cut(s) 542, 566, 1148
HpyF3I CTNAG 7 cut(s) 100, 141, 495, 504, 650, 666, 840
HpySE526I ACGT 2 cut(s) 167, 981
Hsp92I GRCGYC 2 cut(s) 529, 1040
Hsp92II CATG 5 cut(s) 190, 227, 488, 707, 934
Kpn2I TCCGGA 1 cut(s) 589
Kzo9I GATC 3 cut(s) 14, 794, 1155
LmnI GCTCC 2 cut(s) 353, 478
Lsp1109I GCAGC 4 cut(s) 937, 1060, 1129, 1153
LweI GCATC 2 cut(s) 140, 373
MaeI CTAG 3 cut(s) 377, 894, 1163
MaeII ACGT 2 cut(s) 167, 981
MaeIII GTNAC 4 cut(s) 520, 787, 832, 933
MalI GATC 3 cut(s) 16, 796, 1157
MbiI CCGCTC 1 cut(s) 356
MboI GATC 3 cut(s) 14, 794, 1155
MboII GAAGA 7 cut(s) 338, 449, 953, 1048, 1073, 1184, 1210
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 494
MluCI AATT 5 cut(s) 194, 209, 585, 1017, 1207
MlyI GAGTC 4 cut(s) 419, 472, 693, 829
MmeI TCCRAC 1 cut(s) 500
MroI TCCGGA 1 cut(s) 589
MseI TTAA 5 cut(s) 600, 911, 1016, 1020, 1210
MslI CAYNNNNRTG 1 cut(s) 1031
MspI CCGG 4 cut(s) 161, 515, 590, 938
MvnI CGCG 1 cut(s) 745
MwoI GCNNNNNNNGC 3 cut(s) 542, 566, 1148
NcoI CCATGG 1 cut(s) 703
NdeII GATC 3 cut(s) 14, 794, 1155
NlaIII CATG 5 cut(s) 190, 227, 488, 707, 934
NlaIV GGNNCC 2 cut(s) 294, 341
NmuCI GTSAC 4 cut(s) 520, 787, 832, 933
NruI TCGCGA 1 cut(s) 745
NspI RCATGY 1 cut(s) 934
NspV TTCGAA 1 cut(s) 582
OliI CACNNNNGTG 1 cut(s) 1031
PacI TTAATTAA 1 cut(s) 1020
PceI AGGCCT 1 cut(s) 419
PciI ACATGT 1 cut(s) 930
PfeI GAWTC 1 cut(s) 880
PkrI GCNGC 5 cut(s) 358, 952, 1075, 1144, 1168
PleI GAGTC 4 cut(s) 419, 472, 693, 829
PpsI GAGTC 4 cut(s) 419, 472, 693, 829
PpuMI RGGWCCY 1 cut(s) 340
PscI ACATGT 1 cut(s) 930
PshBI ATTAAT 1 cut(s) 911
Psp5II RGGWCCY 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 294, 341
PspPI GGNCC 1 cut(s) 340
PspPPI RGGWCCY 1 cut(s) 340
PstI CTGCAG 1 cut(s) 1033
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 1 cut(s) 14
RruI TCGCGA 1 cut(s) 745
RsaI GTAC 2 cut(s) 801, 1120
RsaNI GTAC 2 cut(s) 800, 1119
RseI CAYNNNNRTG 1 cut(s) 1031
SaqAI TTAA 5 cut(s) 600, 911, 1016, 1020, 1210
SatI GCNGC 5 cut(s) 357, 951, 1074, 1143, 1167
Sau3AI GATC 3 cut(s) 14, 794, 1155
Sau96I GGNCC 1 cut(s) 340
SchI GAGTC 4 cut(s) 419, 472, 693, 829
SduI GDGCHC 1 cut(s) 494
SfaNI GCATC 2 cut(s) 140, 373
SfcI CTRYAG 1 cut(s) 1029
SfuI TTCGAA 1 cut(s) 582
SinI GGWCC 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 1031
SpeI ACTAGT 1 cut(s) 893
Sse9I AATT 5 cut(s) 194, 209, 585, 1017, 1207
SseBI AGGCCT 1 cut(s) 419
SsiI CCGC 2 cut(s) 356, 1169
SspMI CTAG 3 cut(s) 377, 894, 1163
StuI AGGCCT 1 cut(s) 419
StyI CCWWGG 2 cut(s) 432, 703
TaaI ACNGT 3 cut(s) 126, 151, 787
TaiI ACGT 2 cut(s) 170, 984
TaqI TCGA 6 cut(s) 77, 177, 263, 303, 582, 915
TasI AATT 5 cut(s) 194, 209, 585, 1017, 1207
TatI WGTACW 1 cut(s) 1118
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 1 cut(s) 880
Tru1I TTAA 5 cut(s) 600, 911, 1016, 1020, 1210
Tru9I TTAA 5 cut(s) 600, 911, 1016, 1020, 1210
TscAI CASTG 4 cut(s) 51, 64, 1033, 1038
TseFI GTSAC 4 cut(s) 520, 787, 832, 933
TseI GCWGC 4 cut(s) 950, 1073, 1142, 1166
Tsp45I GTSAC 4 cut(s) 520, 787, 832, 933
TspDTI ATGAA 3 cut(s) 60, 657, 933
TspRI CASTG 4 cut(s) 51, 64, 1033, 1038
VpaK11BI GGWCC 1 cut(s) 340
VspI ATTAAT 1 cut(s) 911
XapI RAATTY 2 cut(s) 194, 585
XceI RCATGY 1 cut(s) 934
XmiI GTMKAC 2 cut(s) 216, 606
XspI CTAG 3 cut(s) 377, 894, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.