Rh4CG036600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001250
Physical Location & Seq
Forward (+)
7393579 .. 7395842
2264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG036600.1

Sequence Viewer

Length: 1266 bp
ATGTCATCACCAAGGTCCTCCTCCTCTTCAGCATGTTTATCGCCATCATCGACCGGTAGCACTTCACTTGCATCCTTGGCCACAACTGCACCGGTTTCCTCACCACCCACGTTTGACACGGTACTGTCGTCATCCTCATGTGAGCCGGCAGTGTCACAACCACCCCAATTTGTAACACCAGTTTCACCGACGCCGTTCGAATTAGACCAGATCATGGGCGACTTTCCACGGGCGCAAATGGTGGACTACCATGAGATACAAAGACGCCAAGTGGTCTCTCCATCTCCTTCTTCAAAGGAATTGGTTCTTTATAAGCCGGAGACACAGCGCCAAGAAAACCTGCAGGAACGACTTGGAGAAGTCGTCAAGGATGCTGTTTATAAAGAGGTGACTAAGTTGATGAAACAAGCAGAGGCAGAGATAGCGAAGGAACAAAAGGCTGAGCACGACAGTGGCAGAACTAGCATGCAGGAAGAGGCCTCTGTATTTACGAGGGAAGAGGGTAAACTGATGATAGTAGTGGCAGAGCAGGCTGAGGCTAACCATGAGAGCCCTAAAGTAACCAACACGGAACCGGCTGTCACTACAAGGCCAGGCCGATCGGCCACCAAGCAAGTGGCGCATATTTCCAATGTGTCCTTTCAAATTCCTGATGATTTCGATTTTGTGCCTAATGATGAAGTCCTCAGGGAAATTACTGCTCAGAGTAGGAAAAAATATAATTTCTCTTACAGACTCCATGGATGGAAAATGGAGTGTAGAATAAGACTGAACGGCGTTCGACAATTTGACAAGTATTTTACGCATGTACAATCCAAGGTTGTGACTAGATCAATTCCCGAGGTCGTAAGGTTTATCCTTTATGAGGTAACCCCTAAACAAATGAGGGAAGCCAACAGCAAACGCAAAATGGTAGATTTCTTGGAAGCAGCTGATGAGAAAGCTCAAAATATATTAAAGGATGAATACTCAAGCATGTCATCTGAAGGCGCTGCCAGTGATTCTGAAGAATATGGGGAGTCGTACGAGGAGATGTTTTTTCGCGTAGCCTGGAGTAACCAGATAAACGGCACTAGAGAGGTGGTGCCGTTATTAAGCGAATGGGAAAAATTCAAGGCTGCGAAAAAGAAGGCTAGAACTACACCATGTAAACCAGAAGTTGGTACACCCAACTTGGATGCTGAAGCTATGGAGACAGCAAGCGCCTTGATGGGACTAGCTGAGAATGAAAGTTCGGTGAGGAGAAGGCCGAAGGCTTGTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

421

Amino Acids

47.12

Weight (kDa)

5.77

Isoelectric Point (pI)

59.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 312, 381
Acc36I ACCTGC 1 cut(s) 348
AccB1I GGYRCC 1 cut(s) 1084
AccB7I CCANNNNNTGG 2 cut(s) 214, 1160
AccII CGCG 1 cut(s) 1044
AcoI YGGCCR 2 cut(s) 78, 603
AcsI RAATTY 2 cut(s) 645, 1109
AcuI CTGAAG 4 cut(s) 12, 1005, 1026, 1203
AcyI GRCGYC 2 cut(s) 191, 265
AfaI GTAC 4 cut(s) 123, 810, 1025, 1165
AfiI CCNNNNNNNGG 3 cut(s) 214, 865, 1160
AgeI ACCGGT 2 cut(s) 53, 91
AgsI TTSAA 3 cut(s) 294, 644, 1114
AjnI CCWGG 2 cut(s) 592, 1049
AleI CACNNNNGTG 1 cut(s) 450
AluBI AGCT 4 cut(s) 932, 944, 1187, 1220
AluI AGCT 4 cut(s) 932, 944, 1187, 1220
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 3 cut(s) 280, 314, 1187
Ama87I CYCGRG 1 cut(s) 839
AoxI GGCC 6 cut(s) 78, 477, 590, 595, 603, 1247
ApeKI GCWGC 3 cut(s) 929, 992, 1118
ApoI RAATTY 2 cut(s) 645, 1109
AsiGI ACCGGT 2 cut(s) 53, 91
Asp700I GAANNNNTTC 1 cut(s) 303
AspLEI GCGC 5 cut(s) 235, 330, 622, 992, 1205
AspS9I GGNCC 1 cut(s) 15
AsuHPI GGTGA 4 cut(s) 93, 177, 400, 1249
AsuII TTCGAA 1 cut(s) 198
AvaI CYCGRG 1 cut(s) 839
AvaII GGWCC 1 cut(s) 15
AxyI CCTNAGG 1 cut(s) 686
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BalI TGGCCA 1 cut(s) 80
BanI GGYRCC 1 cut(s) 1084
BanII GRGCYC 1 cut(s) 554
Bbv12I GWGCWC 1 cut(s) 447
BbvCI CCTCAGC 1 cut(s) 534
BbvI GCAGC 3 cut(s) 941, 979, 1105
BccI CCATC 4 cut(s) 52, 289, 738, 1204
BceAI ACGGC 4 cut(s) 178, 790, 1072, 1084
BcgI CGANNNNNNTGC 2 cut(s) 21, 55
BciT130I CCWGG 2 cut(s) 594, 1051
BcoDI GTCTC 3 cut(s) 280, 314, 1187
BfaI CTAG 6 cut(s) 462, 828, 1074, 1134, 1217, 1264
BfmI CTRYAG 1 cut(s) 341
BfoI RGCGCY 3 cut(s) 331, 993, 1206
BfuAI ACCTGC 1 cut(s) 348
BisI GCNGC 3 cut(s) 930, 993, 1119
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 3 cut(s) 931, 994, 1120
Bme1390I CCNGG 2 cut(s) 594, 1051
Bme18I GGWCC 1 cut(s) 15
BmeT110I CYCGRG 1 cut(s) 839
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 2 cut(s) 573, 1086
BmrFI CCNGG 2 cut(s) 594, 1051
BmsI GCATC 3 cut(s) 80, 361, 1168
BpmI CTGGAG 1 cut(s) 1072
Bpu10I CCTNAGC 1 cut(s) 534
Bpu1102I GCTNAGC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 955
BsaHI GRCGYC 2 cut(s) 191, 265
BsaI GGTCTC 1 cut(s) 280
BsaJI CCNNGG 6 cut(s) 11, 75, 227, 739, 816, 840
BsaWI WCCGGW 2 cut(s) 53, 91
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 865, 1160
Bse118I RCCGGY 4 cut(s) 53, 91, 145, 574
Bse1I ACTGG 2 cut(s) 179, 996
Bse21I CCTNAGG 1 cut(s) 686
BseBI CCWGG 2 cut(s) 594, 1051
BseDI CCNNGG 6 cut(s) 11, 75, 227, 739, 816, 840
BseGI GGATG 6 cut(s) 71, 131, 376, 749, 967, 1183
BseLI CCNNNNNNNGG 3 cut(s) 214, 865, 1160
BseMII CTCAG 5 cut(s) 432, 525, 700, 716, 1212
BseNI ACTGG 2 cut(s) 179, 996
BseRI GAGGAG 4 cut(s) 10, 13, 1043, 1255
BseXI GCAGC 3 cut(s) 941, 979, 1105
BsgI GTGCAG 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 1044
Bsh1285I CGRYCG 2 cut(s) 54, 602
BshFI GGCC 6 cut(s) 80, 479, 592, 597, 605, 1249
BshNI GGYRCC 1 cut(s) 1084
BshTI ACCGGT 2 cut(s) 53, 91
BsiEI CGRYCG 2 cut(s) 54, 602
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 839
BsiSI CCGG 5 cut(s) 54, 92, 146, 317, 575
BsiWI CGTACG 1 cut(s) 1023
BslFI GGGAC 1 cut(s) 1227
BslI CCNNNNNNNGG 3 cut(s) 214, 865, 1160
BsmAI GTCTC 3 cut(s) 280, 314, 1187
BsmFI GGGAC 1 cut(s) 1227
BsnI GGCC 6 cut(s) 80, 479, 592, 597, 605, 1249
Bso31I GGTCTC 1 cut(s) 280
BsoBI CYCGRG 1 cut(s) 839
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 2 cut(s) 447, 554
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 3 cut(s) 210, 599, 830
Bsp1720I GCTNAGC 1 cut(s) 441
Bsp19I CCATGG 1 cut(s) 739
BspANI GGCC 6 cut(s) 80, 479, 592, 597, 605, 1249
BspCNI CTCAG 5 cut(s) 433, 526, 699, 715, 1213
BspFNI CGCG 1 cut(s) 1044
BspLI GGNNCC 2 cut(s) 573, 1086
BspMAI CTGCAG 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 348
BspT104I TTCGAA 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 1084
BspTNI GGTCTC 1 cut(s) 280
BsrFI RCCGGY 4 cut(s) 53, 91, 145, 574
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 2 cut(s) 179, 996
BssAI RCCGGY 4 cut(s) 53, 91, 145, 574
BssECI CCNNGG 6 cut(s) 11, 75, 227, 739, 816, 840
BssMI GATC 3 cut(s) 210, 599, 830
BssNI GRCGYC 2 cut(s) 191, 265
BssT1I CCWWGG 4 cut(s) 11, 75, 739, 816
Bst2UI CCWGG 2 cut(s) 594, 1051
Bst4CI ACNGT 3 cut(s) 121, 126, 452
Bst6I CTCTTC 3 cut(s) 31, 468, 492
BstACI GRCGYC 2 cut(s) 191, 265
BstAUI TGTACA 1 cut(s) 808
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 4 cut(s) 147, 467, 531, 1201
BstDEI CTNAG 6 cut(s) 393, 441, 534, 686, 702, 1221
BstDSI CCRYGG 2 cut(s) 227, 739
BstEII GGTNACC 1 cut(s) 868
BstENI CCTNNNNNAGG 1 cut(s) 863
BstF5I GGATG 6 cut(s) 71, 131, 376, 749, 967, 1183
BstFNI CGCG 1 cut(s) 1044
BstH2I RGCGCY 3 cut(s) 331, 993, 1206
BstHHI GCGC 5 cut(s) 235, 330, 622, 992, 1205
BstKTI GATC 3 cut(s) 213, 602, 833
BstMAI GTCTC 3 cut(s) 280, 314, 1187
BstMBI GATC 3 cut(s) 210, 599, 830
BstMCI CGRYCG 2 cut(s) 54, 602
BstMWI GCNNNNNNNGC 6 cut(s) 77, 86, 422, 462, 530, 619
BstNI CCWGG 2 cut(s) 594, 1051
BstNSI RCATGY 4 cut(s) 36, 469, 809, 979
BstPI GGTNACC 1 cut(s) 868
BstSCI CCNGG 2 cut(s) 592, 1049
BstSFI CTRYAG 1 cut(s) 341
BstUI CGCG 1 cut(s) 1044
BstV1I GCAGC 3 cut(s) 941, 979, 1105
BstXI CCANNNNNNTGG 1 cut(s) 616
Bsu36I CCTNAGG 1 cut(s) 686
BsuRI GGCC 6 cut(s) 80, 479, 592, 597, 605, 1249
BtgI CCRYGG 2 cut(s) 227, 739
BtsCI GGATG 6 cut(s) 71, 131, 376, 749, 967, 1183
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 3 cut(s) 156, 457, 1003
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 4 cut(s) 147, 467, 531, 1201
CfoI GCGC 5 cut(s) 235, 330, 622, 992, 1205
Cfr10I RCCGGY 4 cut(s) 53, 91, 145, 574
Cfr13I GGNCC 1 cut(s) 15
CseI GACGC 2 cut(s) 199, 273
Csp6I GTAC 4 cut(s) 122, 809, 1024, 1164
CspAI ACCGGT 2 cut(s) 53, 91
CviQI GTAC 4 cut(s) 122, 809, 1024, 1164
DdeI CTNAG 6 cut(s) 393, 441, 534, 686, 702, 1221
DpnI GATC 3 cut(s) 212, 601, 832
DpnII GATC 3 cut(s) 210, 599, 830
EaeI YGGCCR 2 cut(s) 78, 603
Eam1104I CTCTTC 3 cut(s) 31, 468, 492
EarI CTCTTC 3 cut(s) 31, 468, 492
Eco130I CCWWGG 4 cut(s) 11, 75, 739, 816
Eco147I AGGCCT 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 554
Eco31I GGTCTC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 15
Eco57I CTGAAG 4 cut(s) 12, 1005, 1026, 1203
Eco81I CCTNAGG 1 cut(s) 686
Eco88I CYCGRG 1 cut(s) 839
Eco91I GGTNACC 1 cut(s) 868
EcoNI CCTNNNNNAGG 1 cut(s) 863
EcoO109I RGGNCCY 1 cut(s) 15
EcoO65I GGTNACC 1 cut(s) 868
EcoRII CCWGG 2 cut(s) 592, 1049
EcoT14I CCWWGG 4 cut(s) 11, 75, 739, 816
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 4 cut(s) 11, 75, 739, 816
FaqI GGGAC 1 cut(s) 1227
Fnu4HI GCNGC 3 cut(s) 930, 993, 1119
FokI GGATG 6 cut(s) 58, 118, 383, 756, 974, 1190
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 3 cut(s) 930, 993, 1119
FspBI CTAG 6 cut(s) 462, 828, 1074, 1134, 1217, 1264
GlaI GCGC 5 cut(s) 234, 329, 621, 991, 1204
GluI GCNGC 3 cut(s) 930, 993, 1119
GsuI CTGGAG 1 cut(s) 1072
HaeII RGCGCY 3 cut(s) 331, 993, 1206
HaeIII GGCC 6 cut(s) 80, 479, 592, 597, 605, 1249
HapII CCGG 5 cut(s) 54, 92, 146, 317, 575
HgaI GACGC 2 cut(s) 199, 273
HhaI GCGC 5 cut(s) 235, 330, 622, 992, 1205
Hin1I GRCGYC 2 cut(s) 191, 265
Hin6I GCGC 5 cut(s) 233, 328, 620, 990, 1203
HinP1I GCGC 5 cut(s) 233, 328, 620, 990, 1203
HinfI GANTC 3 cut(s) 735, 1001, 1019
HpaII CCGG 5 cut(s) 54, 92, 146, 317, 575
HphI GGTGA 4 cut(s) 93, 177, 400, 1249
Hpy166II GTNNAC 4 cut(s) 244, 506, 1151, 1166
Hpy188I TCNGA 3 cut(s) 705, 985, 1006
Hpy188III TCNNGA 2 cut(s) 650, 839
Hpy8I GTNNAC 4 cut(s) 244, 506, 1151, 1166
Hpy99I CGWCG 1 cut(s) 193
HpyAV CCTTC 6 cut(s) 297, 421, 980, 1123, 1239, 1246
HpyCH4III ACNGT 3 cut(s) 121, 126, 452
HpyCH4IV ACGT 1 cut(s) 110
HpyCH4V TGCA 4 cut(s) 71, 89, 343, 469
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 86, 422, 462, 530, 619
HpyF3I CTNAG 6 cut(s) 393, 441, 534, 686, 702, 1221
HpySE526I ACGT 1 cut(s) 110
Hsp92I GRCGYC 2 cut(s) 191, 265
HspAI GCGC 5 cut(s) 233, 328, 620, 990, 1203
KroI GCCGGC 1 cut(s) 145
KroNI GCCGGC 1 cut(s) 147
Kzo9I GATC 3 cut(s) 210, 599, 830
Lsp1109I GCAGC 3 cut(s) 941, 979, 1105
LweI GCATC 3 cut(s) 80, 361, 1168
MaeI CTAG 6 cut(s) 462, 828, 1074, 1134, 1217, 1264
MaeII ACGT 1 cut(s) 110
MaeIII GTNAC 8 cut(s) 153, 172, 388, 559, 580, 823, 868, 1055
MalI GATC 3 cut(s) 212, 601, 832
MboI GATC 3 cut(s) 210, 599, 830
MboII GAAGA 5 cut(s) 18, 282, 485, 509, 1019
MhlI GDGCHC 2 cut(s) 447, 554
MlsI TGGCCA 1 cut(s) 80
MluCI AATT 9 cut(s) 167, 200, 299, 645, 693, 721, 785, 834, 1109
MluNI TGGCCA 1 cut(s) 80
MlyI GAGTC 2 cut(s) 729, 1028
Mox20I TGGCCA 1 cut(s) 80
MroNI GCCGGC 1 cut(s) 145
MroXI GAANNNNTTC 1 cut(s) 303
MscI TGGCCA 1 cut(s) 80
MseI TTAA 2 cut(s) 956, 1094
MslI CAYNNNNRTG 2 cut(s) 136, 450
Msp20I TGGCCA 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 932
MspI CCGG 5 cut(s) 54, 92, 146, 317, 575
MspR9I CCNGG 2 cut(s) 594, 1051
MvaI CCWGG 2 cut(s) 594, 1051
MvnI CGCG 1 cut(s) 1044
MwoI GCNNNNNNNGC 6 cut(s) 77, 86, 422, 462, 530, 619
NaeI GCCGGC 1 cut(s) 147
NcoI CCATGG 1 cut(s) 739
NdeII GATC 3 cut(s) 210, 599, 830
NgoMIV GCCGGC 1 cut(s) 145
NlaIV GGNNCC 2 cut(s) 573, 1086
NmuCI GTSAC 4 cut(s) 153, 388, 580, 823
NspI RCATGY 4 cut(s) 36, 469, 809, 979
NspV TTCGAA 1 cut(s) 198
OliI CACNNNNGTG 1 cut(s) 450
PaeI GCATGC 1 cut(s) 469
PceI AGGCCT 1 cut(s) 479
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 125
PdiI GCCGGC 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 1001
Pfl23II CGTACG 1 cut(s) 1023
PflMI CCANNNNNTGG 2 cut(s) 214, 1160
PinAI ACCGGT 2 cut(s) 53, 91
PkrI GCNGC 3 cut(s) 931, 994, 1120
Ple19I CGATCG 1 cut(s) 602
PleI GAGTC 2 cut(s) 729, 1027
PpsI GAGTC 2 cut(s) 729, 1027
PpuMI RGGWCCY 1 cut(s) 15
PsiI TTATAA 2 cut(s) 312, 381
Psp5II RGGWCCY 1 cut(s) 15
Psp6I CCWGG 2 cut(s) 592, 1049
PspEI GGTNACC 1 cut(s) 868
PspGI CCWGG 2 cut(s) 592, 1049
PspLI CGTACG 1 cut(s) 1023
PspN4I GGNNCC 2 cut(s) 573, 1086
PspPI GGNCC 1 cut(s) 15
PspPPI RGGWCCY 1 cut(s) 15
PstI CTGCAG 1 cut(s) 345
PvuI CGATCG 1 cut(s) 602
PvuII CAGCTG 1 cut(s) 932
RsaI GTAC 4 cut(s) 123, 810, 1025, 1165
RsaNI GTAC 4 cut(s) 122, 809, 1024, 1164
RseI CAYNNNNRTG 2 cut(s) 136, 450
SaqAI TTAA 2 cut(s) 956, 1094
SatI GCNGC 3 cut(s) 930, 993, 1119
Sau3AI GATC 3 cut(s) 210, 599, 830
Sau96I GGNCC 1 cut(s) 15
SbfI CCTGCAGG 1 cut(s) 345
SchI GAGTC 2 cut(s) 729, 1028
ScrFI CCNGG 2 cut(s) 594, 1051
SdaI CCTGCAGG 1 cut(s) 345
SduI GDGCHC 2 cut(s) 447, 554
SfaNI GCATC 3 cut(s) 80, 361, 1168
SfcI CTRYAG 1 cut(s) 341
SfuI TTCGAA 1 cut(s) 198
SinI GGWCC 1 cut(s) 15
SmiMI CAYNNNNRTG 2 cut(s) 136, 450
SmlI CTYRAG 1 cut(s) 970
SmoI CTYRAG 1 cut(s) 970
SphI GCATGC 1 cut(s) 469
Sse8387I CCTGCAGG 1 cut(s) 345
Sse9I AATT 9 cut(s) 167, 200, 299, 645, 693, 721, 785, 834, 1109
SseBI AGGCCT 1 cut(s) 479
SspMI CTAG 6 cut(s) 462, 828, 1074, 1134, 1217, 1264
StuI AGGCCT 1 cut(s) 479
StyD4I CCNGG 2 cut(s) 592, 1049
StyI CCWWGG 4 cut(s) 11, 75, 739, 816
TaaI ACNGT 3 cut(s) 121, 126, 452
TaiI ACGT 1 cut(s) 113
TaqI TCGA 4 cut(s) 50, 198, 660, 781
TasI AATT 9 cut(s) 167, 200, 299, 645, 693, 721, 785, 834, 1109
TatI WGTACW 1 cut(s) 808
TfiI GAWTC 1 cut(s) 1001
Tru1I TTAA 2 cut(s) 956, 1094
Tru9I TTAA 2 cut(s) 956, 1094
TscAI CASTG 3 cut(s) 156, 457, 1003
TseFI GTSAC 4 cut(s) 153, 388, 580, 823
TseI GCWGC 3 cut(s) 929, 992, 1118
Tsp45I GTSAC 4 cut(s) 153, 388, 580, 823
TspDTI ATGAA 4 cut(s) 416, 693, 978, 1242
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 3 cut(s) 156, 457, 1003
Van91I CCANNNNNTGG 2 cut(s) 214, 1160
VpaK11BI GGWCC 1 cut(s) 15
XagI CCTNNNNNAGG 1 cut(s) 863
XapI RAATTY 2 cut(s) 645, 1109
XceI RCATGY 4 cut(s) 36, 469, 809, 979
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 6 cut(s) 462, 828, 1074, 1134, 1217, 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.