Rorug03G0191200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001250
Physical Location & Seq
Reverse (-)
16809924 .. 16810766
843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0191200.1

Sequence Viewer

Length: 759 bp
ATGGCTCCAAAAACCACTCTCCACGCTCTTTGGTCTTGTAAGAAAGTCAAGCAGGTTTGGTATCTCTCTCCATTTCATAATGCTATTGGGAGTTGGAAGAATGGAACCTTTTTGGAGTTATTCATTTATGCTACACCTCTCTCTTTAGTGTCTGAGTTGTCTTTTATTGTGGTCATGTGTCGACGATTATGGCAAAACCGTAGTGAAAAGTACTTTCAATGTAAATTTATTAGCCCTCAGTTAATCCACTTCCAAGTTCAACATCAGGTGCAAGAATTTGTCGCTGCAATTCTACCTTCCCCTCTTGGTTCATCTGTATCTACTGCTGCTACTATTGAACATTGGGTTAAACCTCTCTTTCCTTACTTGAAACTTAATGTTGATGGTGCGGTTGATTTAAAGGCTGATTTACGCAATTTGGGGGCAGTGTTAAGGAATGATAGAGGTGAGATGATGCTGGCAGTTTCTAAAGGAATGCAAGGTCGGTTCACTCCCAAGGCCACTGAATTATTTGCTGCAATATTGGGGCTGCAAGCTTGTATTGAGACAGGTTTCCAATCTTCTTCTTTGATGTTGGAAATGGATGCATTGGAGGTGATCCGTGATTTACATGAAGAGGAAGACAACATGTCTATGGAGGGTGCTCTTATAGAAGAAGTGAAGACTCTTTTCCGCATCTTCTCTCCGGCTTGGGTAGAGGATGGACCTCATTGGCTATCCGAAGTACTTATATCCGATGTAAATATCTCAATTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

252

Amino Acids

28.44

Weight (kDa)

5.81

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 126 - 228 2.3e-12 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 181
AciI CCGC 2 cut(s) 389, 673
AclWI GGATC 1 cut(s) 592
AcsI RAATTY 2 cut(s) 224, 275
AfaI GTAC 2 cut(s) 212, 726
AflIII ACRYGT 1 cut(s) 627
AgsI TTSAA 4 cut(s) 218, 260, 338, 370
AhdI GACNNNNNGTC 1 cut(s) 628
AluBI AGCT 1 cut(s) 536
AluI AGCT 1 cut(s) 536
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 1 cut(s) 539
AlwI GGATC 1 cut(s) 592
AoxI GGCC 1 cut(s) 498
ApeKI GCWGC 4 cut(s) 284, 326, 515, 529
ApoI RAATTY 2 cut(s) 224, 275
AspS9I GGNCC 1 cut(s) 704
AsuHPI GGTGA 2 cut(s) 458, 607
AvaII GGWCC 1 cut(s) 704
BbsI GAAGAC 2 cut(s) 627, 668
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 4 cut(s) 271, 313, 502, 516
BccI CCATC 2 cut(s) 377, 695
BcoDI GTCTC 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 43
BisI GCNGC 4 cut(s) 285, 327, 516, 530
BlsI GCNGC 4 cut(s) 286, 328, 517, 531
BmcAI AGTACT 2 cut(s) 212, 726
Bme18I GGWCC 1 cut(s) 704
BmeRI GACNNNNNGTC 1 cut(s) 628
BmgT120I GGNCC 1 cut(s) 704
BmiI GGNNCC 2 cut(s) 6, 106
BmsI GCATC 3 cut(s) 444, 574, 684
BpiI GAAGAC 2 cut(s) 627, 668
BsaJI CCNNGG 1 cut(s) 495
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 2 cut(s) 589, 706
BseMII CTCAG 2 cut(s) 144, 251
BseXI GCAGC 4 cut(s) 271, 313, 502, 516
BshFI GGCC 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 646
BsiSI CCGG 1 cut(s) 686
BsmAI GTCTC 1 cut(s) 539
BsmI GAATGC 1 cut(s) 480
BsnI GGCC 1 cut(s) 500
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 1 cut(s) 597
BspACI CCGC 2 cut(s) 389, 673
BspANI GGCC 1 cut(s) 500
BspCNI CTCAG 2 cut(s) 145, 250
BspLI GGNNCC 2 cut(s) 6, 106
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 1 cut(s) 592
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 495
Bst4CI ACNGT 1 cut(s) 200
Bst6I CTCTTC 1 cut(s) 609
BstC8I GCNNGC 2 cut(s) 459, 534
BstDEI CTNAG 3 cut(s) 153, 237, 756
BstF5I GGATG 2 cut(s) 589, 706
BstKTI GATC 1 cut(s) 600
BstMAI GTCTC 1 cut(s) 539
BstMBI GATC 1 cut(s) 597
BstNSI RCATGY 1 cut(s) 631
BstV1I GCAGC 4 cut(s) 271, 313, 502, 516
BstV2I GAAGAC 2 cut(s) 627, 668
BsuRI GGCC 1 cut(s) 500
BtsCI GGATG 2 cut(s) 589, 706
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 2 cut(s) 432, 501
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 2 cut(s) 459, 534
Cfr13I GGNCC 1 cut(s) 704
Csp6I GTAC 2 cut(s) 211, 725
CspCI CAANNNNNGTGG 2 cut(s) 11, 46
CviAII CATG 3 cut(s) 175, 611, 628
CviJI RGCY 8 cut(s) 5, 234, 404, 500, 529, 536, 689, 715
CviKI_1 RGCY 8 cut(s) 5, 234, 404, 500, 529, 536, 689, 715
CviQI GTAC 2 cut(s) 211, 725
DdeI CTNAG 3 cut(s) 153, 237, 756
DpnI GATC 1 cut(s) 599
DpnII GATC 1 cut(s) 597
DraI TTTAAA 1 cut(s) 399
DriI GACNNNNNGTC 1 cut(s) 628
Eam1104I CTCTTC 1 cut(s) 609
Eam1105I GACNNNNNGTC 1 cut(s) 628
EarI CTCTTC 1 cut(s) 609
Eco130I CCWWGG 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 704
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 1 cut(s) 589
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 3 cut(s) 178, 614, 631
FaiI YATR 9 cut(s) 78, 129, 176, 190, 612, 629, 635, 650, 731
FatI CATG 3 cut(s) 174, 610, 627
FblI GTMKAC 1 cut(s) 181
Fnu4HI GCNGC 4 cut(s) 285, 327, 516, 530
FokI GGATG 2 cut(s) 596, 713
Fsp4HI GCNGC 4 cut(s) 285, 327, 516, 530
GluI GCNGC 4 cut(s) 285, 327, 516, 530
HaeIII GGCC 1 cut(s) 500
HapII CCGG 1 cut(s) 686
Hin1II CATG 3 cut(s) 178, 614, 631
HincII GTYRAC 1 cut(s) 182
HindII GTYRAC 1 cut(s) 182
HindIII AAGCTT 1 cut(s) 534
HinfI GANTC 1 cut(s) 664
HpaII CCGG 1 cut(s) 686
HphI GGTGA 2 cut(s) 458, 607
Hpy166II GTNNAC 2 cut(s) 182, 489
Hpy188I TCNGA 3 cut(s) 154, 721, 736
Hpy8I GTNNAC 2 cut(s) 182, 489
Hpy99I CGWCG 1 cut(s) 186
HpyAV CCTTC 1 cut(s) 306
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4V TGCA 6 cut(s) 271, 287, 478, 518, 532, 587
HpyF3I CTNAG 3 cut(s) 153, 237, 756
Hsp92II CATG 3 cut(s) 178, 614, 631
Kzo9I GATC 1 cut(s) 597
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 5 cut(s) 38, 251, 443, 534, 699
Lsp1109I GCAGC 4 cut(s) 271, 313, 502, 516
LweI GCATC 3 cut(s) 444, 574, 684
MalI GATC 1 cut(s) 599
MboI GATC 1 cut(s) 597
MboII GAAGA 8 cut(s) 109, 552, 555, 626, 632, 665, 670, 673
MfeI CAATTG 1 cut(s) 750
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 6 cut(s) 224, 275, 288, 415, 506, 750
MlyI GAGTC 1 cut(s) 658
MmeI TCCRAC 2 cut(s) 74, 555
Mph1103I ATGCAT 1 cut(s) 589
MseI TTAA 5 cut(s) 242, 348, 375, 398, 431
MslI CAYNNNNRTG 1 cut(s) 632
MspI CCGG 1 cut(s) 686
MunI CAATTG 1 cut(s) 750
Mva1269I GAATGC 1 cut(s) 480
NdeII GATC 1 cut(s) 597
NlaIII CATG 3 cut(s) 178, 614, 631
NlaIV GGNNCC 2 cut(s) 6, 106
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 1 cut(s) 631
PciI ACATGT 1 cut(s) 627
PctI GAATGC 1 cut(s) 480
PkrI GCNGC 4 cut(s) 286, 328, 517, 531
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
PscI ACATGT 1 cut(s) 627
PspN4I GGNNCC 2 cut(s) 6, 106
PspPI GGNCC 1 cut(s) 704
RsaI GTAC 2 cut(s) 212, 726
RsaNI GTAC 2 cut(s) 211, 725
RseI CAYNNNNRTG 1 cut(s) 632
SalI GTCGAC 1 cut(s) 180
SaqAI TTAA 5 cut(s) 242, 348, 375, 398, 431
SatI GCNGC 4 cut(s) 285, 327, 516, 530
Sau3AI GATC 1 cut(s) 597
Sau96I GGNCC 1 cut(s) 704
ScaI AGTACT 2 cut(s) 212, 726
SchI GAGTC 1 cut(s) 658
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 3 cut(s) 444, 574, 684
SinI GGWCC 1 cut(s) 704
SmiMI CAYNNNNRTG 1 cut(s) 632
Sse9I AATT 6 cut(s) 224, 275, 288, 415, 506, 750
SsiI CCGC 2 cut(s) 389, 673
SspI AATATT 1 cut(s) 522
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 1 cut(s) 200
TaqI TCGA 1 cut(s) 181
TasI AATT 6 cut(s) 224, 275, 288, 415, 506, 750
TatI WGTACW 2 cut(s) 210, 724
Tru1I TTAA 5 cut(s) 242, 348, 375, 398, 431
Tru9I TTAA 5 cut(s) 242, 348, 375, 398, 431
TscAI CASTG 2 cut(s) 432, 508
TseI GCWGC 4 cut(s) 284, 326, 515, 529
TspDTI ATGAA 4 cut(s) 65, 112, 300, 627
TspGWI ACGGA 1 cut(s) 590
TspRI CASTG 2 cut(s) 432, 508
VpaK11BI GGWCC 1 cut(s) 704
XapI RAATTY 2 cut(s) 224, 275
XceI RCATGY 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 181
ZrmI AGTACT 2 cut(s) 212, 726
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.