Rh4AG032000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001250
Physical Location & Seq
Reverse (-)
6912786 .. 6915408
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG032000.1

Sequence Viewer

Length: 1269 bp
ATGGCATCACCCAGATCCTCCACTTCAGCATGGTTGTCCCCATCATCGACTAGTAGCACTTCACTTGCATCCTCGGCTACAACTGCACCGGATCCCTTACCACCCAAGTTTGACACGGTACTGTCGTCATCGCCATGTCAGCCGGTAGTGTCACCACCACCCCAATCTGTAACACCAGTTTCACAGAAGCCGTTCGATTTAGATCAGGTCATGGGCAACTTTCCACGGGCGCAAATAGTGGACTACCATGAGACACAAAGACGCCAAGTGGTCTCTCCATCTCCTTCTTCAAAGGAATTGGTTCTTTATAAGCCGGAGACACAGCGACAAGAAAATCTACAGGAACGACTTGGGCAAGTCATCAAGGATCTTGTTCATAAAGAGGTGACCGAGTTGATGAAACAAGCAGAGGCGGAGATAGCGAACGAACGAAAGGCTGAGCATTACAGTGGCAGAAGTAGCATGGAAGAGGCGTCTGAGTCAACGAAGGAAGACAATAAACTGATGATAGTAGCGGCAGAGGAGGCTCAGGCTGACCATGGGAGCCCTAAAATAACGAACACGGAACCGACCTTCACTACCAGGCCAGGCCGATCAGCCACGAAACTAATGGCCCATATTTCCAATGTGTCTTTCCAAATGCCTGATGATTTCGAAGTTGACGATGACACAATAAATCAGTTTGTGCCTAATGATGAAGTGCTCAGGGAAATTACTGCTCAAGGTAGGAAAAAATATAGTTTCTTTGACAGACTCCATGGATGGAAAATGGAGTGTAGATTAAGACTGAATGGCGTTCGACAATTCGATAAGTATTTCACACATGTTCAATCGAAGATTGTGACGAGATCAATTCCTGAAGTTGTGAGGTTTATCCTTTATGAGGTGAGCCCTAAACAAATGAGGGAAGCCAACAGCAAACGTAAATCGATAGATTTTTGGGAGCCAGCTAATGAGAGCCCTAAAAATATATTAAAGGATGAATACCCAAGTATGTCATCTGAAGGCGCTGCTGGTGATTTTGAAGAAGAGGAGGTGTCGTACGTGCAGATGTTTCTTCGCGTAGCCTGGAGTAACCTGATGAACGGCACTGCAGAGGAGGTGCCATTGTTAAGTGAATGGGAAAAATTCAAGGCTGCAAAAAAGAAGGCCAGAACTACACCTTGCAAACCAGAGGTTGGTACATCCAACTTGGATGCTGAAGCTGTAGAGACAGCAAACGCCTTGATGGGACTAGCTGACGATGAAATGAATGATGAAGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

422

Amino Acids

47.35

Weight (kDa)

5.14

Isoelectric Point (pI)

57.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 1102
AccB7I CCANNNNNTGG 1 cut(s) 1178
AccII CGCG 1 cut(s) 1062
AciI CCGC 2 cut(s) 413, 515
AclWI GGATC 4 cut(s) 9, 86, 99, 375
AcsI RAATTY 1 cut(s) 1127
AcuI CTGAAG 4 cut(s) 9, 879, 1023, 1221
AcyI GRCGYC 2 cut(s) 262, 473
AfaI GTAC 3 cut(s) 120, 1043, 1183
AfiI CCNNNNNNNGG 2 cut(s) 883, 1178
AflIII ACRYGT 1 cut(s) 823
AgsI TTSAA 4 cut(s) 291, 830, 1025, 1132
AhlI ACTAGT 1 cut(s) 50
AjnI CCWGG 3 cut(s) 581, 586, 1067
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AluBI AGCT 3 cut(s) 950, 1205, 1238
AluI AGCT 3 cut(s) 950, 1205, 1238
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 4 cut(s) 245, 277, 311, 1205
AlwI GGATC 4 cut(s) 9, 86, 99, 375
AoxI GGCC 4 cut(s) 584, 589, 612, 1149
ApeKI GCWGC 2 cut(s) 1010, 1136
ApoI RAATTY 1 cut(s) 1127
Asp700I GAANNNNTTC 2 cut(s) 191, 300
AspLEI GCGC 2 cut(s) 232, 1010
AspS9I GGNCC 1 cut(s) 613
AsuHPI GGTGA 4 cut(s) 144, 397, 898, 1028
AsuII TTCGAA 1 cut(s) 654
BaeI ACNNNNGTAYC 2 cut(s) 110, 143
BamHI GGATCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 1102
BanII GRGCYC 3 cut(s) 548, 893, 962
BbsI GAAGAC 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 705
BbvI GCAGC 2 cut(s) 997, 1123
BccI CCATC 4 cut(s) 49, 286, 756, 1222
BceAI ACGGC 2 cut(s) 175, 1102
BciT130I CCWGG 3 cut(s) 583, 588, 1069
BcoDI GTCTC 4 cut(s) 245, 277, 311, 1205
BcuI ACTAGT 1 cut(s) 50
BfaI CTAG 2 cut(s) 51, 1235
BfmI CTRYAG 3 cut(s) 338, 1092, 1206
BfoI RGCGCY 1 cut(s) 1011
BisI GCNGC 3 cut(s) 516, 1011, 1137
BlpI GCTNAGC 1 cut(s) 438
BlsI GCNGC 3 cut(s) 517, 1012, 1138
Bme1390I CCNGG 3 cut(s) 583, 588, 1069
BmgT120I GGNCC 1 cut(s) 613
BmiI GGNNCC 5 cut(s) 93, 545, 567, 945, 1104
BmrFI CCNGG 3 cut(s) 583, 588, 1069
BmsI GCATC 3 cut(s) 14, 77, 1186
BpiI GAAGAC 1 cut(s) 498
BpmI CTGGAG 1 cut(s) 1090
Bpu10I CCTNAGC 2 cut(s) 528, 704
Bpu1102I GCTNAGC 1 cut(s) 438
Bpu14I TTCGAA 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 705
Bsa29I ATCGAT 1 cut(s) 929
BsaAI YACGTR 1 cut(s) 1045
BsaBI GATNNNNATC 1 cut(s) 201
BsaHI GRCGYC 2 cut(s) 262, 473
BsaI GGTCTC 1 cut(s) 277
BsaJI CCNNGG 4 cut(s) 72, 224, 538, 757
BsaWI WCCGGW 1 cut(s) 88
BsaXI ACNNNNNCTCC 2 cut(s) 1025, 1055
Bsc4I CCNNNNNNNGG 2 cut(s) 883, 1178
Bse118I RCCGGY 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 176
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 3 cut(s) 583, 588, 1069
BseCI ATCGAT 1 cut(s) 929
BseDI CCNNGG 4 cut(s) 72, 224, 538, 757
BseGI GGATG 5 cut(s) 68, 767, 985, 1184, 1201
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 2 cut(s) 883, 1178
BseMII CTCAG 4 cut(s) 429, 468, 542, 718
BseNI ACTGG 1 cut(s) 176
BseRI GAGGAG 3 cut(s) 536, 1046, 1112
BseXI GCAGC 2 cut(s) 997, 1123
BsgI GTGCAG 2 cut(s) 69, 1067
Bsh1236I CGCG 1 cut(s) 1062
BshFI GGCC 4 cut(s) 586, 591, 614, 1151
BshNI GGYRCC 1 cut(s) 1102
BshVI ATCGAT 1 cut(s) 929
BsiHKAI GWGCWC 1 cut(s) 705
BsiSI CCGG 3 cut(s) 89, 143, 314
BsiWI CGTACG 1 cut(s) 1041
BslFI GGGAC 2 cut(s) 22, 1245
BslI CCNNNNNNNGG 2 cut(s) 883, 1178
BsmAI GTCTC 4 cut(s) 245, 277, 311, 1205
BsmFI GGGAC 2 cut(s) 22, 1245
BsnI GGCC 4 cut(s) 586, 591, 614, 1151
Bso31I GGTCTC 1 cut(s) 277
Bsp119I TTCGAA 1 cut(s) 654
Bsp1286I GDGCHC 4 cut(s) 548, 705, 893, 962
Bsp143I GATC 6 cut(s) 14, 91, 202, 367, 593, 848
Bsp1720I GCTNAGC 1 cut(s) 438
Bsp19I CCATGG 2 cut(s) 538, 757
BspACI CCGC 2 cut(s) 413, 515
BspANI GGCC 4 cut(s) 586, 591, 614, 1151
BspCNI CTCAG 4 cut(s) 430, 469, 541, 717
BspDI ATCGAT 1 cut(s) 929
BspFNI CGCG 1 cut(s) 1062
BspLI GGNNCC 5 cut(s) 93, 545, 567, 945, 1104
BspMAI CTGCAG 1 cut(s) 1096
BspPI GGATC 4 cut(s) 9, 86, 99, 375
BspT104I TTCGAA 1 cut(s) 654
BspT107I GGYRCC 1 cut(s) 1102
BspTNI GGTCTC 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 142
BsrI ACTGG 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 4 cut(s) 72, 224, 538, 757
BssMI GATC 6 cut(s) 14, 91, 202, 367, 593, 848
BssNI GRCGYC 2 cut(s) 262, 473
BssT1I CCWWGG 2 cut(s) 538, 757
Bst2UI CCWGG 3 cut(s) 583, 588, 1069
Bst4CI ACNGT 3 cut(s) 118, 123, 449
Bst6I CTCTTC 2 cut(s) 462, 1023
BstACI GRCGYC 2 cut(s) 262, 473
BstBAI YACGTR 1 cut(s) 1045
BstBI TTCGAA 1 cut(s) 654
BstC8I GCNNGC 1 cut(s) 948
BstDEI CTNAG 4 cut(s) 438, 477, 528, 704
BstDSI CCRYGG 3 cut(s) 224, 538, 757
BstEII GGTNACC 1 cut(s) 385
BstENI CCTNNNNNAGG 1 cut(s) 881
BstF5I GGATG 5 cut(s) 68, 767, 985, 1184, 1201
BstFNI CGCG 1 cut(s) 1062
BstH2I RGCGCY 1 cut(s) 1011
BstHHI GCGC 2 cut(s) 232, 1010
BstKTI GATC 6 cut(s) 17, 94, 205, 370, 596, 851
BstMAI GTCTC 4 cut(s) 245, 277, 311, 1205
BstMBI GATC 6 cut(s) 14, 91, 202, 367, 593, 848
BstMWI GCNNNNNNNGC 6 cut(s) 74, 83, 139, 419, 459, 524
BstNI CCWGG 3 cut(s) 583, 588, 1069
BstNSI RCATGY 1 cut(s) 827
BstPI GGTNACC 1 cut(s) 385
BstSCI CCNGG 3 cut(s) 581, 586, 1067
BstSFI CTRYAG 3 cut(s) 338, 1092, 1206
BstUI CGCG 1 cut(s) 1062
BstV1I GCAGC 2 cut(s) 997, 1123
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 3 cut(s) 14, 91, 367
BstYI RGATCY 3 cut(s) 14, 91, 367
Bsu15I ATCGAT 1 cut(s) 929
BsuRI GGCC 4 cut(s) 586, 591, 614, 1151
BsuTUI ATCGAT 1 cut(s) 929
BtgI CCRYGG 3 cut(s) 224, 538, 757
BtgZI GCGATG 1 cut(s) 114
BtsCI GGATG 5 cut(s) 68, 767, 985, 1184, 1201
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 2 cut(s) 454, 1089
Cac8I GCNNGC 1 cut(s) 948
CfoI GCGC 2 cut(s) 232, 1010
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 613
ClaI ATCGAT 1 cut(s) 929
CseI GACGC 2 cut(s) 270, 462
Csp6I GTAC 3 cut(s) 119, 1042, 1182
CviAII CATG 8 cut(s) 30, 135, 211, 248, 463, 539, 758, 824
CviQI GTAC 3 cut(s) 119, 1042, 1182
DdeI CTNAG 4 cut(s) 438, 477, 528, 704
DpnI GATC 6 cut(s) 16, 93, 204, 369, 595, 850
DpnII GATC 6 cut(s) 14, 91, 202, 367, 593, 848
Eam1104I CTCTTC 2 cut(s) 462, 1023
EarI CTCTTC 2 cut(s) 462, 1023
EciI GGCGGA 1 cut(s) 428
Eco130I CCWWGG 2 cut(s) 538, 757
Eco24I GRGCYC 3 cut(s) 548, 893, 962
Eco31I GGTCTC 1 cut(s) 277
Eco57I CTGAAG 4 cut(s) 9, 879, 1023, 1221
Eco91I GGTNACC 1 cut(s) 385
EcoNI CCTNNNNNAGG 1 cut(s) 881
EcoO65I GGTNACC 1 cut(s) 385
EcoRII CCWGG 3 cut(s) 581, 586, 1067
EcoT14I CCWWGG 2 cut(s) 538, 757
EcoT38I GRGCYC 3 cut(s) 548, 893, 962
ErhI CCWWGG 2 cut(s) 538, 757
FaeI CATG 8 cut(s) 33, 138, 214, 251, 466, 542, 761, 827
FaqI GGGAC 2 cut(s) 22, 1245
FatI CATG 8 cut(s) 29, 134, 210, 247, 462, 538, 757, 823
Fnu4HI GCNGC 3 cut(s) 516, 1011, 1137
FokI GGATG 5 cut(s) 55, 774, 992, 1171, 1208
FriOI GRGCYC 3 cut(s) 548, 893, 962
Fsp4HI GCNGC 3 cut(s) 516, 1011, 1137
FspBI CTAG 2 cut(s) 51, 1235
GlaI GCGC 2 cut(s) 231, 1009
GluI GCNGC 3 cut(s) 516, 1011, 1137
GsuI CTGGAG 1 cut(s) 1090
HaeII RGCGCY 1 cut(s) 1011
HaeIII GGCC 4 cut(s) 586, 591, 614, 1151
HapII CCGG 3 cut(s) 89, 143, 314
HgaI GACGC 2 cut(s) 270, 462
HhaI GCGC 2 cut(s) 232, 1010
Hin1I GRCGYC 2 cut(s) 262, 473
Hin1II CATG 8 cut(s) 33, 138, 214, 251, 466, 542, 761, 827
Hin6I GCGC 2 cut(s) 230, 1008
HinP1I GCGC 2 cut(s) 230, 1008
HincII GTYRAC 2 cut(s) 483, 661
HindII GTYRAC 2 cut(s) 483, 661
HinfI GANTC 2 cut(s) 479, 753
HpaII CCGG 3 cut(s) 89, 143, 314
HphI GGTGA 4 cut(s) 144, 397, 898, 1028
Hpy166II GTNNAC 3 cut(s) 241, 483, 661
Hpy188I TCNGA 2 cut(s) 478, 1003
Hpy188III TCNNGA 1 cut(s) 857
Hpy8I GTNNAC 3 cut(s) 241, 483, 661
HpyAV CCTTC 6 cut(s) 294, 481, 583, 998, 1141, 1253
HpyCH4III ACNGT 3 cut(s) 118, 123, 449
HpyCH4IV ACGT 2 cut(s) 922, 1044
HpyCH4V TGCA 6 cut(s) 68, 86, 1048, 1094, 1139, 1167
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 83, 139, 419, 459, 524
HpyF3I CTNAG 4 cut(s) 438, 477, 528, 704
HpySE526I ACGT 2 cut(s) 922, 1044
Hsp92I GRCGYC 2 cut(s) 262, 473
Hsp92II CATG 8 cut(s) 33, 138, 214, 251, 466, 542, 761, 827
HspAI GCGC 2 cut(s) 230, 1008
Kzo9I GATC 6 cut(s) 14, 91, 202, 367, 593, 848
LmnI GCTCC 2 cut(s) 543, 943
Lsp1109I GCAGC 2 cut(s) 997, 1123
LweI GCATC 3 cut(s) 14, 77, 1186
MaeI CTAG 2 cut(s) 51, 1235
MaeII ACGT 2 cut(s) 922, 1044
MaeIII GTNAC 5 cut(s) 150, 169, 385, 841, 1073
MalI GATC 6 cut(s) 16, 93, 204, 369, 595, 850
MboI GATC 6 cut(s) 14, 91, 202, 367, 593, 848
MboII GAAGA 7 cut(s) 279, 479, 503, 847, 1037, 1040, 1049
MflI RGATCY 3 cut(s) 14, 91, 367
MhlI GDGCHC 4 cut(s) 548, 705, 893, 962
MluCI AATT 5 cut(s) 296, 711, 803, 852, 1127
MlyI GAGTC 2 cut(s) 488, 747
MmeI TCCRAC 1 cut(s) 1212
MroXI GAANNNNTTC 2 cut(s) 191, 300
MseI TTAA 3 cut(s) 782, 974, 1112
MslI CAYNNNNRTG 2 cut(s) 133, 447
MspI CCGG 3 cut(s) 89, 143, 314
MspR9I CCNGG 3 cut(s) 583, 588, 1069
MvaI CCWGG 3 cut(s) 583, 588, 1069
MvnI CGCG 1 cut(s) 1062
MwoI GCNNNNNNNGC 6 cut(s) 74, 83, 139, 419, 459, 524
NcoI CCATGG 2 cut(s) 538, 757
NdeII GATC 6 cut(s) 14, 91, 202, 367, 593, 848
NlaIII CATG 8 cut(s) 33, 138, 214, 251, 466, 542, 761, 827
NlaIV GGNNCC 5 cut(s) 93, 545, 567, 945, 1104
NmeAIII GCCGAG 1 cut(s) 53
NmuCI GTSAC 3 cut(s) 150, 385, 841
NspI RCATGY 1 cut(s) 827
NspV TTCGAA 1 cut(s) 654
PciI ACATGT 1 cut(s) 823
PcsI WCGNNNNNNNCGW 2 cut(s) 122, 660
PdmI GAANNNNTTC 2 cut(s) 191, 300
Pfl23II CGTACG 1 cut(s) 1041
PflMI CCANNNNNTGG 1 cut(s) 1178
PkrI GCNGC 3 cut(s) 517, 1012, 1138
PleI GAGTC 2 cut(s) 487, 747
PpsI GAGTC 2 cut(s) 487, 747
Ppu21I YACGTR 1 cut(s) 1045
PscI ACATGT 1 cut(s) 823
PsiI TTATAA 1 cut(s) 309
Psp6I CCWGG 3 cut(s) 581, 586, 1067
PspEI GGTNACC 1 cut(s) 385
PspGI CCWGG 3 cut(s) 581, 586, 1067
PspLI CGTACG 1 cut(s) 1041
PspN4I GGNNCC 5 cut(s) 93, 545, 567, 945, 1104
PspPI GGNCC 1 cut(s) 613
PstI CTGCAG 1 cut(s) 1096
PsuI RGATCY 3 cut(s) 14, 91, 367
RsaI GTAC 3 cut(s) 120, 1043, 1183
RsaNI GTAC 3 cut(s) 119, 1042, 1182
RseI CAYNNNNRTG 2 cut(s) 133, 447
SaqAI TTAA 3 cut(s) 782, 974, 1112
SatI GCNGC 3 cut(s) 516, 1011, 1137
Sau3AI GATC 6 cut(s) 14, 91, 202, 367, 593, 848
Sau96I GGNCC 1 cut(s) 613
SchI GAGTC 2 cut(s) 488, 747
ScrFI CCNGG 3 cut(s) 583, 588, 1069
SduI GDGCHC 4 cut(s) 548, 705, 893, 962
SfaNI GCATC 3 cut(s) 14, 77, 1186
SfcI CTRYAG 3 cut(s) 338, 1092, 1206
SfuI TTCGAA 1 cut(s) 654
SmiMI CAYNNNNRTG 2 cut(s) 133, 447
SmlI CTYRAG 1 cut(s) 720
SmoI CTYRAG 1 cut(s) 720
SpeI ACTAGT 1 cut(s) 50
Sse9I AATT 5 cut(s) 296, 711, 803, 852, 1127
SsiI CCGC 2 cut(s) 413, 515
SspMI CTAG 2 cut(s) 51, 1235
StyD4I CCNGG 3 cut(s) 581, 586, 1067
StyI CCWWGG 2 cut(s) 538, 757
TaaI ACNGT 3 cut(s) 118, 123, 449
TaiI ACGT 2 cut(s) 925, 1047
TaqI TCGA 7 cut(s) 47, 195, 654, 799, 807, 833, 929
TaqII GACCGA 1 cut(s) 404
TasI AATT 5 cut(s) 296, 711, 803, 852, 1127
TauI GCSGC 1 cut(s) 518
Tru1I TTAA 3 cut(s) 782, 974, 1112
Tru9I TTAA 3 cut(s) 782, 974, 1112
TscAI CASTG 2 cut(s) 454, 1096
TseFI GTSAC 3 cut(s) 150, 385, 841
TseI GCWGC 2 cut(s) 1010, 1136
Tsp45I GTSAC 3 cut(s) 150, 385, 841
TspDTI ATGAA 7 cut(s) 365, 413, 711, 996, 1097, 1260, 1265
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 2 cut(s) 454, 1096
Van91I CCANNNNNTGG 1 cut(s) 1178
XagI CCTNNNNNAGG 1 cut(s) 881
XapI RAATTY 1 cut(s) 1127
XceI RCATGY 1 cut(s) 827
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmnI GAANNNNTTC 2 cut(s) 191, 300
XspI CTAG 2 cut(s) 51, 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.