Rh4DG028400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001250
Physical Location & Seq
Reverse (-)
5036905 .. 5049277
12373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG028400.1

Sequence Viewer

Length: 1050 bp
ATGGCATCACCAAGATCCTCCTTTTTCTCAACGTGGAGGTCGCCATCATCCCCGTCACTGAACCGTAGCACTTCACTTGCATCCCCGGCTACAACTGCACCGGTTCCCTCACCACCCACTTTTGAGACTGGACTGTCGCCATCCACATATGAGCCGACAGTGACACCAGAACCCCAATTTTCACCCATGCCGTTCGATTACGATCAGATCATGGATGACTTTTCACCGGTGAAAATAGTGGACTACCATGAGATGCAAAGAGGCCAAGTGGTCTCTCCATCTCCTTCTTCAAATGAATTAGTTCTCAATGAACCGAAGACACAGCCACAAGAAAATATACAGGAACGACTCGGACAAGTTGTCAAGGGTCTTGTTCAAAAAGAGGTAGATAAGTTGATGAAACAAGCAAAGGCGGACAGTGGCAGAACTAGCGTGGAAGAGACCTCTGAGTTAGCGAAGGACGACGGTAAATTGAAGATAACAGCGGCAGAGAAGGCTCAGGCTGACCATGAGAGCCCTAAAATAACTAACATGGAACCGACCTTCACTACCAGGCCAGGCCGATCGGCCACCAAGCAAGTGTTTGCTCCTAAGGATGAAGTCCTCAGAGAAATTACTGCTCAGAGTAGGAAAAAGTATAGTTTCTCTGACAGACTCCATGGATGGAAAATGGAGTGTAGGATAAGACTGAATGGTATCCAACAATTCGATAAGTATTTTACGCATGTAGAATCGAAGATTGGGACAAGATCAGTTCCCGAGGTTAGGGAATCGTACGATGAGTTGGTTTTTCGCGTAGCCTGGACTAATCTGATAAACGACGCTGCAGAGAAGGTGCCGTTATTTAGTGAATGGGAAAAATTCAAGGTTGCAAAAAAGAAGGCCAGAACTAAACCATGCGAACCTGAGGTTGGTACACCCAACTTGGATATTGAAGCTCTAGAGACAGCAAAGGCCTTGATGGGACTAGCTGAGGATGAAAGTTTGGCTACGAGAAAGTCGAAGGCTTGTAATGATAGGTCTGGTTTTGGTGTTTTTGTCAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

349

Amino Acids

39.08

Weight (kDa)

7.65

Isoelectric Point (pI)

57.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 835
AccII CGCG 1 cut(s) 795
AciI CCGC 2 cut(s) 413, 485
AclWI GGATC 1 cut(s) 9
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 1 cut(s) 860
AfaI GTAC 2 cut(s) 776, 916
AfiI CCNNNNNNNGG 2 cut(s) 765, 911
AgeI ACCGGT 2 cut(s) 100, 226
AgsI TTSAA 5 cut(s) 291, 377, 475, 865, 935
AhdI GACNNNNNGTC 1 cut(s) 359
AjnI CCWGG 3 cut(s) 551, 556, 800
AjuI GAANNNNNNNTTGG 4 cut(s) 723, 755, 894, 926
AluBI AGCT 2 cut(s) 938, 971
AluI AGCT 2 cut(s) 938, 971
Alw26I GTCTC 4 cut(s) 119, 277, 434, 938
AlwI GGATC 1 cut(s) 9
Ama87I CYCGRG 1 cut(s) 758
AoxI GGCC 6 cut(s) 262, 554, 559, 567, 882, 954
ApeKI GCWGC 1 cut(s) 824
ApoI RAATTY 1 cut(s) 860
AsiGI ACCGGT 2 cut(s) 100, 226
Asp700I GAANNNNTTC 1 cut(s) 300
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 4 cut(s) 102, 174, 216, 241
AvaI CYCGRG 1 cut(s) 758
AxyI CCTNAGG 2 cut(s) 591, 906
BanI GGYRCC 1 cut(s) 835
BanII GRGCYC 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 323
BbvCI CCTCAGC 1 cut(s) 972
BbvI GCAGC 1 cut(s) 811
BccI CCATC 5 cut(s) 52, 148, 286, 657, 955
BceAI ACGGC 2 cut(s) 175, 823
BciT130I CCWGG 3 cut(s) 553, 558, 802
BciVI GTATCC 1 cut(s) 707
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 4 cut(s) 119, 277, 434, 938
BfaI CTAG 3 cut(s) 429, 941, 968
BfmI CTRYAG 1 cut(s) 825
BfuI GTATCC 1 cut(s) 707
BisI GCNGC 2 cut(s) 486, 825
BlsI GCNGC 2 cut(s) 487, 826
Bme1390I CCNGG 4 cut(s) 86, 553, 558, 802
BmeRI GACNNNNNGTC 1 cut(s) 359
BmeT110I CYCGRG 1 cut(s) 758
BmiI GGNNCC 3 cut(s) 105, 537, 837
BmrFI CCNGG 4 cut(s) 86, 553, 558, 802
BmsI GCATC 3 cut(s) 14, 89, 243
BpiI GAAGAC 1 cut(s) 323
Bpu10I CCTNAGC 2 cut(s) 498, 972
BpuMI CCSGG 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 201
BsaI GGTCTC 2 cut(s) 277, 434
BsaJI CCNNGG 3 cut(s) 84, 658, 759
BsaWI WCCGGW 2 cut(s) 100, 226
Bsc4I CCNNNNNNNGG 2 cut(s) 765, 911
Bse118I RCCGGY 2 cut(s) 100, 226
Bse1I ACTGG 1 cut(s) 133
Bse21I CCTNAGG 2 cut(s) 591, 906
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 3 cut(s) 553, 558, 802
BseDI CCNNGG 3 cut(s) 84, 658, 759
BseGI GGATG 7 cut(s) 47, 80, 140, 220, 601, 668, 982
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 2 cut(s) 765, 911
BseMII CTCAG 6 cut(s) 438, 512, 619, 635, 897, 963
BseNI ACTGG 1 cut(s) 133
BseXI GCAGC 1 cut(s) 811
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 795
Bsh1285I CGRYCG 1 cut(s) 566
BshFI GGCC 6 cut(s) 264, 556, 561, 569, 884, 956
BshNI GGYRCC 1 cut(s) 835
BshTI ACCGGT 2 cut(s) 100, 226
BsiEI CGRYCG 1 cut(s) 566
BsiHKCI CYCGRG 1 cut(s) 758
BsiSI CCGG 3 cut(s) 86, 101, 227
BsiWI CGTACG 1 cut(s) 774
BslFI GGGAC 2 cut(s) 757, 978
BslI CCNNNNNNNGG 2 cut(s) 765, 911
BsmAI GTCTC 4 cut(s) 119, 277, 434, 938
BsmFI GGGAC 2 cut(s) 757, 978
BsnI GGCC 6 cut(s) 264, 556, 561, 569, 884, 956
Bso31I GGTCTC 2 cut(s) 277, 434
BsoBI CYCGRG 1 cut(s) 758
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 5 cut(s) 14, 202, 207, 563, 749
Bsp19I CCATGG 1 cut(s) 658
BspACI CCGC 2 cut(s) 413, 485
BspANI GGCC 6 cut(s) 264, 556, 561, 569, 884, 956
BspCNI CTCAG 6 cut(s) 439, 511, 618, 634, 898, 964
BspFNI CGCG 1 cut(s) 795
BspLI GGNNCC 3 cut(s) 105, 537, 837
BspMAI CTGCAG 1 cut(s) 829
BspPI GGATC 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 835
BspTNI GGTCTC 2 cut(s) 277, 434
BsrFI RCCGGY 2 cut(s) 100, 226
BsrI ACTGG 1 cut(s) 133
BssAI RCCGGY 2 cut(s) 100, 226
BssECI CCNNGG 3 cut(s) 84, 658, 759
BssMI GATC 5 cut(s) 14, 202, 207, 563, 749
BssT1I CCWWGG 1 cut(s) 658
Bst2UI CCWGG 3 cut(s) 553, 558, 802
Bst4CI ACNGT 5 cut(s) 65, 135, 160, 419, 467
Bst6I CTCTTC 1 cut(s) 432
BstDEI CTNAG 7 cut(s) 447, 498, 591, 605, 621, 906, 972
BstDSI CCRYGG 1 cut(s) 658
BstF5I GGATG 7 cut(s) 47, 80, 140, 220, 601, 668, 982
BstFNI CGCG 1 cut(s) 795
BstKTI GATC 5 cut(s) 17, 205, 210, 566, 752
BstMAI GTCTC 4 cut(s) 119, 277, 434, 938
BstMBI GATC 5 cut(s) 14, 202, 207, 563, 749
BstMCI CGRYCG 1 cut(s) 566
BstMWI GCNNNNNNNGC 4 cut(s) 86, 95, 429, 494
BstNI CCWGG 3 cut(s) 553, 558, 802
BstNSI RCATGY 1 cut(s) 728
BstSCI CCNGG 4 cut(s) 84, 551, 556, 800
BstSFI CTRYAG 1 cut(s) 825
BstUI CGCG 1 cut(s) 795
BstV1I GCAGC 1 cut(s) 811
BstV2I GAAGAC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
Bsu36I CCTNAGG 2 cut(s) 591, 906
BsuI GTATCC 1 cut(s) 707
BsuRI GGCC 6 cut(s) 264, 556, 561, 569, 884, 956
BtgI CCRYGG 1 cut(s) 658
BtsCI GGATG 7 cut(s) 47, 80, 140, 220, 601, 668, 982
BtsIMutI CAGTG 3 cut(s) 56, 165, 424
Cfr10I RCCGGY 2 cut(s) 100, 226
CseI GACGC 1 cut(s) 830
Csp6I GTAC 2 cut(s) 775, 915
CspAI ACCGGT 2 cut(s) 100, 226
CviAII CATG 8 cut(s) 187, 211, 248, 509, 532, 659, 725, 897
CviQI GTAC 2 cut(s) 775, 915
DdeI CTNAG 7 cut(s) 447, 498, 591, 605, 621, 906, 972
DpnI GATC 5 cut(s) 16, 204, 209, 565, 751
DpnII GATC 5 cut(s) 14, 202, 207, 563, 749
DrdI GACNNNNNNGTC 1 cut(s) 133
DriI GACNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 1 cut(s) 567
Eam1104I CTCTTC 1 cut(s) 432
Eam1105I GACNNNNNGTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 432
EciI GGCGGA 1 cut(s) 428
Eco130I CCWWGG 1 cut(s) 658
Eco147I AGGCCT 1 cut(s) 956
Eco24I GRGCYC 1 cut(s) 518
Eco31I GGTCTC 2 cut(s) 277, 434
Eco81I CCTNAGG 2 cut(s) 591, 906
Eco88I CYCGRG 1 cut(s) 758
EcoRII CCWGG 3 cut(s) 551, 556, 800
EcoT14I CCWWGG 1 cut(s) 658
EcoT38I GRGCYC 1 cut(s) 518
ErhI CCWWGG 1 cut(s) 658
FaeI CATG 8 cut(s) 190, 214, 251, 512, 535, 662, 728, 900
FaqI GGGAC 2 cut(s) 757, 978
FatI CATG 8 cut(s) 186, 210, 247, 508, 531, 658, 724, 896
FauNDI CATATG 1 cut(s) 148
Fnu4HI GCNGC 2 cut(s) 486, 825
FokI GGATG 7 cut(s) 34, 67, 127, 227, 608, 675, 989
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 2 cut(s) 486, 825
FspBI CTAG 3 cut(s) 429, 941, 968
GluI GCNGC 2 cut(s) 486, 825
HaeIII GGCC 6 cut(s) 264, 556, 561, 569, 884, 956
HapII CCGG 3 cut(s) 86, 101, 227
HgaI GACGC 1 cut(s) 830
Hin1II CATG 8 cut(s) 190, 214, 251, 512, 535, 662, 728, 900
HinfI GANTC 4 cut(s) 348, 654, 731, 770
HpaII CCGG 3 cut(s) 86, 101, 227
HphI GGTGA 4 cut(s) 102, 174, 216, 241
Hpy166II GTNNAC 2 cut(s) 241, 917
Hpy188I TCNGA 7 cut(s) 207, 353, 448, 608, 624, 649, 813
Hpy188III TCNNGA 3 cut(s) 758, 941, 1042
Hpy8I GTNNAC 2 cut(s) 241, 917
Hpy99I CGWCG 2 cut(s) 467, 824
HpyAV CCTTC 7 cut(s) 294, 451, 487, 553, 826, 874, 997
HpyCH4III ACNGT 5 cut(s) 65, 135, 160, 419, 467
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 5 cut(s) 80, 98, 256, 827, 872
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 95, 429, 494
HpyF3I CTNAG 7 cut(s) 447, 498, 591, 605, 621, 906, 972
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 8 cut(s) 190, 214, 251, 512, 535, 662, 728, 900
Kzo9I GATC 5 cut(s) 14, 202, 207, 563, 749
LmnI GCTCC 1 cut(s) 592
Lsp1109I GCAGC 1 cut(s) 811
LweI GCATC 3 cut(s) 14, 89, 243
MaeI CTAG 3 cut(s) 429, 941, 968
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 54, 160
MalI GATC 5 cut(s) 16, 204, 209, 565, 751
MboI GATC 5 cut(s) 14, 202, 207, 563, 749
MboII GAAGA 5 cut(s) 279, 328, 449, 487, 748
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 6 cut(s) 176, 296, 470, 612, 704, 860
MlyI GAGTC 2 cut(s) 342, 648
MmeI TCCRAC 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 3 cut(s) 86, 101, 227
MspR9I CCNGG 4 cut(s) 86, 553, 558, 802
MvaI CCWGG 3 cut(s) 553, 558, 802
MvnI CGCG 1 cut(s) 795
MwoI GCNNNNNNNGC 4 cut(s) 86, 95, 429, 494
NciI CCSGG 1 cut(s) 86
NcoI CCATGG 1 cut(s) 658
NdeI CATATG 1 cut(s) 148
NdeII GATC 5 cut(s) 14, 202, 207, 563, 749
NlaIII CATG 8 cut(s) 190, 214, 251, 512, 535, 662, 728, 900
NlaIV GGNNCC 3 cut(s) 105, 537, 837
NmuCI GTSAC 2 cut(s) 54, 160
NspI RCATGY 1 cut(s) 728
PceI AGGCCT 1 cut(s) 956
PcsI WCGNNNNNNNCGW 1 cut(s) 998
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 2 cut(s) 731, 770
Pfl23II CGTACG 1 cut(s) 774
PinAI ACCGGT 2 cut(s) 100, 226
PkrI GCNGC 2 cut(s) 487, 826
Ple19I CGATCG 1 cut(s) 566
PleI GAGTC 2 cut(s) 342, 648
PpsI GAGTC 2 cut(s) 342, 648
Psp6I CCWGG 3 cut(s) 551, 556, 800
PspGI CCWGG 3 cut(s) 551, 556, 800
PspLI CGTACG 1 cut(s) 774
PspN4I GGNNCC 3 cut(s) 105, 537, 837
PstI CTGCAG 1 cut(s) 829
PsuI RGATCY 1 cut(s) 14
PvuI CGATCG 1 cut(s) 566
RsaI GTAC 2 cut(s) 776, 916
RsaNI GTAC 2 cut(s) 775, 915
SatI GCNGC 2 cut(s) 486, 825
Sau3AI GATC 5 cut(s) 14, 202, 207, 563, 749
SchI GAGTC 2 cut(s) 342, 648
ScrFI CCNGG 4 cut(s) 86, 553, 558, 802
SduI GDGCHC 1 cut(s) 518
SfaNI GCATC 3 cut(s) 14, 89, 243
SfcI CTRYAG 1 cut(s) 825
SgrAI CRCCGGYG 1 cut(s) 226
Sse9I AATT 6 cut(s) 176, 296, 470, 612, 704, 860
SseBI AGGCCT 1 cut(s) 956
SsiI CCGC 2 cut(s) 413, 485
SspMI CTAG 3 cut(s) 429, 941, 968
StuI AGGCCT 1 cut(s) 956
StyD4I CCNGG 4 cut(s) 84, 551, 556, 800
StyI CCWWGG 1 cut(s) 658
TaaI ACNGT 5 cut(s) 65, 135, 160, 419, 467
TaiI ACGT 1 cut(s) 35
TaqI TCGA 4 cut(s) 195, 708, 734, 1001
TasI AATT 6 cut(s) 176, 296, 470, 612, 704, 860
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 2 cut(s) 731, 770
TscAI CASTG 3 cut(s) 63, 165, 424
TseFI GTSAC 2 cut(s) 54, 160
TseI GCWGC 1 cut(s) 824
Tsp45I GTSAC 2 cut(s) 54, 160
TspDTI ATGAA 5 cut(s) 309, 324, 413, 612, 993
TspRI CASTG 3 cut(s) 63, 165, 424
XapI RAATTY 1 cut(s) 860
XbaI TCTAGA 1 cut(s) 940
XceI RCATGY 1 cut(s) 728
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 3 cut(s) 429, 941, 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.