pycom12g13720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001250
Physical Location & Seq
Forward (+)
16206175 .. 16209596
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g13720.3

Sequence Viewer

Length: 1263 bp
ATGTCTATGTCCGCAGAACCCTCATCTCTTGATGTATTTACTGAATCTGAAAATGACCTCTCTAAATTCCCAGAAGCCGAGTTCGTGGAAAGCCATGAGATACAAGCCCGTGTTGATCCAAAAGTAGTAATTAGACCTATGGAGGGCTATAGTAGTAGTATCCGAGAGCAAAAATTGCAACAAGTCAAACGCTTGCTTCGGAAAATGATTTCTGCAAAAACAGCAGAGTTGAAGGCGAGGTGGTCTGATCATGAAAACATGCAAATTGTAGTATACCAACCACCTAAATACCCCATGAATATGACACCAAGCCTCCAAGTAGCACAAGTCCAAGACAAACCGACAAAATCACCGACCACGGTGATCGGAGAGGAGGTGGTGTCATTTGAACTGCCCACAGAATTCTCTGAGGATTTAAAGACGTTGATACCGGAGGGGCCTCCTCACCGATTTTCTTTCTCTGAAAGACTAGATGGCTGGAGGATTACTCAAAGACGACGAACCAAGGGAGGGAAGGACACGTTCTATAGACATGAGAAATCAAACTCTTGCCTGATGAGATCGATTGTCGAGGCGGTTAGATTTATGATGTATGAGAAATTCCCTAAACAAGGAGGGGCAAGCTCAGATGACGAGGAGATAAGGACAAGTTCAGCACCGAGACTGAGAAAAGCTTCGGATGTTGACTGTTACAACAATAAAATCTCAGAGGAAAATGTGCAGGGTTTTGATGGCGAACCCTATACAGAAGAAAGGGTGACAGAATTTTTTAAGTCAACCGAAAGAAAACCTGTGAAGGTTCCGCAGCAAAACCCATCTCCACCAAGCATTTTTTCCTGCAGTTCTTCTCAAGAAGGACATGCTAACAGTCCTTCCGGTCCTTCTATGTTCAGTGGTGTAGAAGTAAGAGCAACTGCAACGGCTATGAGATTACAGGACTTTACAGTCGATATGAGTTATCCGAGAAAAACTATATCATCAGCAGACAACTCTGTCGAGTTTAGATCAAGGGCTGGGAGATCAAGCGCACAAATGGAAGCTAGAGTTGTTGAGGGCTCTAGTTCTAATGACCAAAGTCATGAGTTGCAAGTCAATCTTCACAAAGCATTGTACAATCAAATTCTCCAAAAAGCCAGCACTACTTTGGATGGGATAAATATTGTCGACTCATTCATGCTTCCGGAAGATCTCCCTCTGGATGGTCTCGACATTGAGAACGCAAATTATACATACAGAACAAATGAATTATCTTCTAGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

421

Amino Acids

47.47

Weight (kDa)

5.61

Isoelectric Point (pI)

62.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 944, 992
AccI GTMKAC 2 cut(s) 273, 1164
AccIII TCCGGA 1 cut(s) 1180
AciI CCGC 3 cut(s) 12, 575, 803
AclWI GGATC 1 cut(s) 110
AcsI RAATTY 5 cut(s) 65, 401, 599, 764, 1119
AfaI GTAC 1 cut(s) 1112
AfiI CCNNNNNNNGG 4 cut(s) 143, 510, 611, 1199
AflIII ACRYGT 1 cut(s) 519
AgsI TTSAA 2 cut(s) 232, 389
AjuI GAANNNNNNNTTGG 2 cut(s) 817, 849
AluBI AGCT 3 cut(s) 624, 674, 1040
AluI AGCT 3 cut(s) 624, 674, 1040
Alw26I GTCTC 2 cut(s) 655, 1208
AlwI GGATC 1 cut(s) 110
Aor13HI TCCGGA 1 cut(s) 1180
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 1 cut(s) 805
ApoI RAATTY 5 cut(s) 65, 401, 599, 764, 1119
Asp700I GAANNNNTTC 1 cut(s) 673
AspLEI GCGC 1 cut(s) 1028
AspS9I GGNCC 2 cut(s) 437, 878
AsuHPI GGTGA 4 cut(s) 342, 373, 437, 769
AvaII GGWCC 1 cut(s) 878
BanII GRGCYC 1 cut(s) 1058
BbvI GCAGC 1 cut(s) 817
BccI CCATC 5 cut(s) 467, 725, 823, 1142, 1193
BceAI ACGGC 1 cut(s) 936
BciVI GTATCC 1 cut(s) 170
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 2 cut(s) 655, 1208
BfaI CTAG 4 cut(s) 470, 1041, 1059, 1254
BfmI CTRYAG 3 cut(s) 148, 526, 838
BfuI GTATCC 1 cut(s) 170
BglII AGATCT 1 cut(s) 1186
BisI GCNGC 1 cut(s) 806
BlsI GCNGC 1 cut(s) 807
Bme18I GGWCC 1 cut(s) 878
BmgT120I GGNCC 2 cut(s) 437, 878
BmiI GGNNCC 2 cut(s) 438, 801
BoxI GACNNNNGTC 1 cut(s) 1074
BplI GAGNNNNNCTC 2 cut(s) 472, 504
BpmI CTGGAG 1 cut(s) 499
BpuEI CTTGAG 1 cut(s) 834
Bsa29I ATCGAT 1 cut(s) 563
BsaI GGTCTC 1 cut(s) 1208
BsaJI CCNNGG 2 cut(s) 357, 504
BsaWI WCCGGW 3 cut(s) 430, 875, 1180
BsaXI ACNNNNNCTCC 6 cut(s) 297, 327, 360, 365, 390, 395
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 510, 611, 1199
BseAI TCCGGA 1 cut(s) 1180
BseCI ATCGAT 1 cut(s) 563
BseDI CCNNGG 2 cut(s) 357, 504
BseGI GGATG 3 cut(s) 685, 1153, 1204
BseLI CCNNNNNNNGG 4 cut(s) 143, 510, 611, 1199
BseMII CTCAG 4 cut(s) 399, 639, 656, 720
BseRI GAGGAG 3 cut(s) 386, 432, 650
BseXI GCAGC 1 cut(s) 817
BseYI CCCAGC 1 cut(s) 1013
BsgI GTGCAG 1 cut(s) 740
BshFI GGCC 1 cut(s) 439
BshVI ATCGAT 1 cut(s) 563
BsiSI CCGG 3 cut(s) 431, 876, 1181
BslI CCNNNNNNNGG 4 cut(s) 143, 510, 611, 1199
BsmAI GTCTC 2 cut(s) 655, 1208
BsnI GGCC 1 cut(s) 439
Bso31I GGTCTC 1 cut(s) 1208
Bsp1286I GDGCHC 1 cut(s) 1058
Bsp13I TCCGGA 1 cut(s) 1180
Bsp1407I TGTACA 1 cut(s) 1110
Bsp143I GATC 7 cut(s) 115, 247, 363, 560, 1004, 1019, 1186
BspACI CCGC 3 cut(s) 12, 575, 803
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 4 cut(s) 400, 638, 657, 719
BspDI ATCGAT 1 cut(s) 563
BspEI TCCGGA 1 cut(s) 1180
BspHI TCATGA 2 cut(s) 250, 1078
BspLI GGNNCC 2 cut(s) 438, 801
BspMAI CTGCAG 1 cut(s) 842
BspPI GGATC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 1208
BsrGI TGTACA 1 cut(s) 1110
BssECI CCNNGG 2 cut(s) 357, 504
BssMI GATC 7 cut(s) 115, 247, 363, 560, 1004, 1019, 1186
BssNAI GTATAC 1 cut(s) 274
BssT1I CCWWGG 1 cut(s) 504
Bst1107I GTATAC 1 cut(s) 274
Bst4CI ACNGT 4 cut(s) 361, 689, 869, 946
BstAPI GCANNNNNTGC 1 cut(s) 175
BstAUI TGTACA 1 cut(s) 1110
BstC8I GCNNGC 3 cut(s) 194, 622, 1135
BstDEI CTNAG 4 cut(s) 408, 625, 665, 706
BstDSI CCRYGG 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 609
BstF5I GGATG 3 cut(s) 685, 1153, 1204
BstHHI GCGC 1 cut(s) 1028
BstKTI GATC 7 cut(s) 118, 250, 366, 563, 1007, 1022, 1189
BstMAI GTCTC 2 cut(s) 655, 1208
BstMBI GATC 7 cut(s) 115, 247, 363, 560, 1004, 1019, 1186
BstMWI GCNNNNNNNGC 2 cut(s) 175, 221
BstNSI RCATGY 2 cut(s) 262, 863
BstPAI GACNNNNGTC 1 cut(s) 1074
BstSFI CTRYAG 3 cut(s) 148, 526, 838
BstV1I GCAGC 1 cut(s) 817
BstX2I RGATCY 1 cut(s) 1186
BstYI RGATCY 1 cut(s) 1186
BstZ17I GTATAC 1 cut(s) 274
Bsu15I ATCGAT 1 cut(s) 563
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 1 cut(s) 439
BsuTUI ATCGAT 1 cut(s) 563
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 3 cut(s) 685, 1153, 1204
BtsIMutI CAGTG 1 cut(s) 898
Cac8I GCNNGC 3 cut(s) 194, 622, 1135
CciI TCATGA 2 cut(s) 250, 1078
CfoI GCGC 1 cut(s) 1028
Cfr13I GGNCC 2 cut(s) 437, 878
ClaI ATCGAT 1 cut(s) 563
Csp6I GTAC 1 cut(s) 1111
CviAII CATG 8 cut(s) 95, 251, 259, 295, 533, 860, 1079, 1174
CviQI GTAC 1 cut(s) 1111
DdeI CTNAG 4 cut(s) 408, 625, 665, 706
DpnI GATC 7 cut(s) 117, 249, 365, 562, 1006, 1021, 1188
DpnII GATC 7 cut(s) 115, 247, 363, 560, 1004, 1019, 1186
DraI TTTAAA 1 cut(s) 417
DrdI GACNNNNNNGTC 2 cut(s) 944, 992
DseDI GACNNNNNNGTC 2 cut(s) 944, 992
Eco130I CCWWGG 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 1058
Eco31I GGTCTC 1 cut(s) 1208
Eco47I GGWCC 1 cut(s) 878
EcoNI CCTNNNNNAGG 1 cut(s) 609
EcoO109I RGGNCCY 1 cut(s) 437
EcoRI GAATTC 1 cut(s) 401
EcoT14I CCWWGG 1 cut(s) 504
EcoT38I GRGCYC 1 cut(s) 1058
ErhI CCWWGG 1 cut(s) 504
FaeI CATG 8 cut(s) 98, 254, 262, 298, 536, 863, 1082, 1177
FalI AAGNNNNNCTT 2 cut(s) 1080, 1112
FatI CATG 8 cut(s) 94, 250, 258, 294, 532, 859, 1078, 1173
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 2 cut(s) 273, 1164
Fnu4HI GCNGC 1 cut(s) 806
FokI GGATG 3 cut(s) 692, 1160, 1211
FriOI GRGCYC 1 cut(s) 1058
Fsp4HI GCNGC 1 cut(s) 806
FspBI CTAG 4 cut(s) 470, 1041, 1059, 1254
GlaI GCGC 1 cut(s) 1027
GluI GCNGC 1 cut(s) 806
GsaI CCCAGC 1 cut(s) 1017
GsuI CTGGAG 1 cut(s) 499
HaeIII GGCC 1 cut(s) 439
HapII CCGG 3 cut(s) 431, 876, 1181
HhaI GCGC 1 cut(s) 1028
Hin1II CATG 8 cut(s) 98, 254, 262, 298, 536, 863, 1082, 1177
Hin6I GCGC 1 cut(s) 1026
HinP1I GCGC 1 cut(s) 1026
HincII GTYRAC 3 cut(s) 685, 777, 1165
HindII GTYRAC 3 cut(s) 685, 777, 1165
HindIII AAGCTT 1 cut(s) 672
HinfI GANTC 2 cut(s) 44, 1166
HpaII CCGG 3 cut(s) 431, 876, 1181
HphI GGTGA 4 cut(s) 342, 373, 437, 769
Hpy166II GTNNAC 4 cut(s) 274, 685, 777, 1165
Hpy188III TCNNGA 7 cut(s) 29, 251, 851, 1079, 1181, 1196, 1205
Hpy8I GTNNAC 4 cut(s) 274, 685, 777, 1165
Hpy99I CGWCG 1 cut(s) 501
HpyAV CCTTC 6 cut(s) 226, 508, 790, 848, 882, 891
HpyCH4III ACNGT 4 cut(s) 361, 689, 869, 946
HpyCH4IV ACGT 2 cut(s) 422, 521
HpyCH4V TGCA 7 cut(s) 178, 215, 262, 721, 840, 917, 1087
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 221
HpyF3I CTNAG 4 cut(s) 408, 625, 665, 706
HpySE526I ACGT 2 cut(s) 422, 521
Hsp92II CATG 8 cut(s) 98, 254, 262, 298, 536, 863, 1082, 1177
HspAI GCGC 1 cut(s) 1026
Kpn2I TCCGGA 1 cut(s) 1180
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 7 cut(s) 115, 247, 363, 560, 1004, 1019, 1186
Lsp1109I GCAGC 1 cut(s) 817
MaeI CTAG 4 cut(s) 470, 1041, 1059, 1254
MaeII ACGT 2 cut(s) 422, 521
MaeIII GTNAC 2 cut(s) 689, 757
MalI GATC 7 cut(s) 117, 249, 365, 562, 1006, 1021, 1188
MboI GATC 7 cut(s) 115, 247, 363, 560, 1004, 1019, 1186
MboII GAAGA 5 cut(s) 761, 837, 1088, 1196, 1242
MflI RGATCY 1 cut(s) 1186
MhlI GDGCHC 1 cut(s) 1058
MlyI GAGTC 1 cut(s) 1160
MroI TCCGGA 1 cut(s) 1180
MroXI GAANNNNTTC 1 cut(s) 673
MseI TTAA 2 cut(s) 416, 771
MslI CAYNNNNRTG 1 cut(s) 299
MspI CCGG 3 cut(s) 431, 876, 1181
MwoI GCNNNNNNNGC 2 cut(s) 175, 221
NdeII GATC 7 cut(s) 115, 247, 363, 560, 1004, 1019, 1186
NlaIII CATG 8 cut(s) 98, 254, 262, 298, 536, 863, 1082, 1177
NlaIV GGNNCC 2 cut(s) 438, 801
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 757
NspI RCATGY 2 cut(s) 262, 863
PagI TCATGA 2 cut(s) 250, 1078
PdmI GAANNNNTTC 1 cut(s) 673
PfeI GAWTC 1 cut(s) 44
PkrI GCNGC 1 cut(s) 807
PleI GAGTC 1 cut(s) 1160
PpsI GAGTC 1 cut(s) 1160
PshAI GACNNNNGTC 1 cut(s) 1074
PspFI CCCAGC 1 cut(s) 1013
PspN4I GGNNCC 2 cut(s) 438, 801
PspPI GGNCC 2 cut(s) 437, 878
PstI CTGCAG 1 cut(s) 842
PsuI RGATCY 1 cut(s) 1186
RsaI GTAC 1 cut(s) 1112
RsaNI GTAC 1 cut(s) 1111
RseI CAYNNNNRTG 1 cut(s) 299
SalI GTCGAC 1 cut(s) 1163
SaqAI TTAA 2 cut(s) 416, 771
SatI GCNGC 1 cut(s) 806
Sau3AI GATC 7 cut(s) 115, 247, 363, 560, 1004, 1019, 1186
Sau96I GGNCC 2 cut(s) 437, 878
SchI GAGTC 1 cut(s) 1160
SduI GDGCHC 1 cut(s) 1058
SfcI CTRYAG 3 cut(s) 148, 526, 838
SinI GGWCC 1 cut(s) 878
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 849
SmoI CTYRAG 1 cut(s) 849
SsiI CCGC 3 cut(s) 12, 575, 803
SspI AATATT 1 cut(s) 1159
SspMI CTAG 4 cut(s) 470, 1041, 1059, 1254
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 4 cut(s) 361, 689, 869, 946
TaiI ACGT 2 cut(s) 425, 524
TaqI TCGA 6 cut(s) 563, 570, 948, 996, 1164, 1206
TatI WGTACW 1 cut(s) 1110
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 2 cut(s) 416, 771
Tru9I TTAA 2 cut(s) 416, 771
TscAI CASTG 1 cut(s) 898
TseFI GTSAC 1 cut(s) 757
TseI GCWGC 1 cut(s) 805
Tsp45I GTSAC 1 cut(s) 757
TspDTI ATGAA 4 cut(s) 267, 311, 1162, 1257
TspRI CASTG 1 cut(s) 898
VpaK11BI GGWCC 1 cut(s) 878
XagI CCTNNNNNAGG 1 cut(s) 609
XapI RAATTY 5 cut(s) 65, 401, 599, 764, 1119
XceI RCATGY 2 cut(s) 262, 863
XcmI CCANNNNNNNNNTGG 1 cut(s) 1141
XmiI GTMKAC 2 cut(s) 273, 1164
XmnI GAANNNNTTC 1 cut(s) 673
XspI CTAG 4 cut(s) 470, 1041, 1059, 1254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.