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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 316 |
| AciI | CCGC | 2 cut(s) | 140, 162 |
| AcsI | RAATTY | 1 cut(s) | 362 |
| AdeI | CACNNNGTG | 1 cut(s) | 214 |
| AfaI | GTAC | 2 cut(s) | 12, 343 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 327, 328 |
| AgsI | TTSAA | 3 cut(s) | 104, 369, 377 |
| AjiI | CACGTC | 2 cut(s) | 304, 425 |
| Alw26I | GTCTC | 1 cut(s) | 211 |
| ApoI | RAATTY | 1 cut(s) | 362 |
| AsuHPI | GGTGA | 1 cut(s) | 38 |
| BaeGI | GKGCMC | 2 cut(s) | 321, 430 |
| BanI | GGYRCC | 1 cut(s) | 316 |
| BccI | CCATC | 1 cut(s) | 384 |
| BciVI | GTATCC | 1 cut(s) | 365 |
| BcoDI | GTCTC | 1 cut(s) | 211 |
| BfuI | GTATCC | 1 cut(s) | 365 |
| BglII | AGATCT | 1 cut(s) | 115 |
| BmgBI | CACGTC | 2 cut(s) | 304, 425 |
| BmiI | GGNNCC | 2 cut(s) | 144, 318 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 102, 134 |
| BpuEI | CTTGAG | 1 cut(s) | 368 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 327, 328 |
| Bse1I | ACTGG | 2 cut(s) | 343, 424 |
| BseGI | GGATG | 2 cut(s) | 80, 395 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 327, 328 |
| BseMII | CTCAG | 1 cut(s) | 315 |
| BseNI | ACTGG | 2 cut(s) | 343, 424 |
| BseSI | GKGCMC | 2 cut(s) | 321, 430 |
| BshNI | GGYRCC | 1 cut(s) | 316 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 327, 328 |
| BsmAI | GTCTC | 1 cut(s) | 211 |
| BsmI | GAATGC | 2 cut(s) | 79, 142 |
| Bsp1286I | GDGCHC | 2 cut(s) | 321, 430 |
| Bsp143I | GATC | 2 cut(s) | 115, 438 |
| BspACI | CCGC | 2 cut(s) | 140, 162 |
| BspCNI | CTCAG | 1 cut(s) | 316 |
| BspLI | GGNNCC | 2 cut(s) | 144, 318 |
| BspT107I | GGYRCC | 1 cut(s) | 316 |
| BsrI | ACTGG | 2 cut(s) | 343, 424 |
| BssMI | GATC | 2 cut(s) | 115, 438 |
| Bst4CI | ACNGT | 1 cut(s) | 10 |
| BstDEI | CTNAG | 1 cut(s) | 324 |
| BstF5I | GGATG | 2 cut(s) | 80, 395 |
| BstKTI | GATC | 2 cut(s) | 118, 441 |
| BstMAI | GTCTC | 1 cut(s) | 211 |
| BstMBI | GATC | 2 cut(s) | 115, 438 |
| BstSLI | GKGCMC | 2 cut(s) | 321, 430 |
| BstX2I | RGATCY | 1 cut(s) | 115 |
| BstYI | RGATCY | 1 cut(s) | 115 |
| BsuI | GTATCC | 1 cut(s) | 365 |
| BtrI | CACGTC | 2 cut(s) | 304, 425 |
| BtsCI | GGATG | 2 cut(s) | 80, 395 |
| Csp6I | GTAC | 2 cut(s) | 11, 342 |
| CviJI | RGCY | 2 cut(s) | 5, 382 |
| CviKI_1 | RGCY | 2 cut(s) | 5, 382 |
| CviQI | GTAC | 2 cut(s) | 11, 342 |
| DdeI | CTNAG | 1 cut(s) | 324 |
| DpnI | GATC | 2 cut(s) | 117, 440 |
| DpnII | GATC | 2 cut(s) | 115, 438 |
| DraIII | CACNNNGTG | 1 cut(s) | 214 |
| FaiI | YATR | 1 cut(s) | 233 |
| FauI | CCCGC | 1 cut(s) | 169 |
| FokI | GGATG | 2 cut(s) | 67, 402 |
| HinfI | GANTC | 4 cut(s) | 86, 104, 128, 434 |
| HphI | GGTGA | 1 cut(s) | 38 |
| Hpy188I | TCNGA | 2 cut(s) | 85, 297 |
| Hpy188III | TCNNGA | 1 cut(s) | 108 |
| HpyAV | CCTTC | 2 cut(s) | 167, 299 |
| HpyCH4III | ACNGT | 1 cut(s) | 10 |
| HpyCH4IV | ACGT | 2 cut(s) | 303, 424 |
| HpyCH4V | TGCA | 1 cut(s) | 40 |
| HpyF3I | CTNAG | 1 cut(s) | 324 |
| HpySE526I | ACGT | 2 cut(s) | 303, 424 |
| Kzo9I | GATC | 2 cut(s) | 115, 438 |
| LmnI | GCTCC | 1 cut(s) | 110 |
| LpnPI | CCDG | 8 cut(s) | 3, 93, 110, 255, 288, 324, 336, 405 |
| MaeII | ACGT | 2 cut(s) | 303, 424 |
| MaeIII | GTNAC | 2 cut(s) | 30, 334 |
| MalI | GATC | 2 cut(s) | 117, 440 |
| MboI | GATC | 2 cut(s) | 115, 438 |
| MboII | GAAGA | 3 cut(s) | 106, 149, 193 |
| MflI | RGATCY | 1 cut(s) | 115 |
| MhlI | GDGCHC | 2 cut(s) | 321, 430 |
| MluCI | AATT | 1 cut(s) | 362 |
| MlyI | GAGTC | 2 cut(s) | 95, 428 |
| MmeI | TCCRAC | 2 cut(s) | 177, 292 |
| MnlI | CCTC | 4 cut(s) | 193, 321, 394, 440 |
| Mva1269I | GAATGC | 2 cut(s) | 79, 142 |
| NdeII | GATC | 2 cut(s) | 115, 438 |
| NlaIV | GGNNCC | 2 cut(s) | 144, 318 |
| PctI | GAATGC | 2 cut(s) | 79, 142 |
| PfeI | GAWTC | 2 cut(s) | 104, 128 |
| PleI | GAGTC | 2 cut(s) | 94, 428 |
| PpsI | GAGTC | 2 cut(s) | 94, 428 |
| PspN4I | GGNNCC | 2 cut(s) | 144, 318 |
| PsuI | RGATCY | 1 cut(s) | 115 |
| RsaI | GTAC | 2 cut(s) | 12, 343 |
| RsaNI | GTAC | 2 cut(s) | 11, 342 |
| Sau3AI | GATC | 2 cut(s) | 115, 438 |
| SchI | GAGTC | 2 cut(s) | 95, 428 |
| SduI | GDGCHC | 2 cut(s) | 321, 430 |
| SetI | ASST | 3 cut(s) | 306, 405, 427 |
| SmlI | CTYRAG | 1 cut(s) | 383 |
| SmoI | CTYRAG | 1 cut(s) | 383 |
| Sse9I | AATT | 1 cut(s) | 362 |
| SsiI | CCGC | 2 cut(s) | 140, 162 |
| TaaI | ACNGT | 1 cut(s) | 10 |
| TaiI | ACGT | 2 cut(s) | 306, 427 |
| TaqI | TCGA | 3 cut(s) | 399, 432, 437 |
| TasI | AATT | 1 cut(s) | 362 |
| TatI | WGTACW | 1 cut(s) | 10 |
| TfiI | GAWTC | 2 cut(s) | 104, 128 |
| TspDTI | ATGAA | 3 cut(s) | 186, 252, 281 |
| TspGWI | ACGGA | 1 cut(s) | 347 |
| XapI | RAATTY | 1 cut(s) | 362 |