Rh4DG027800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001250
Physical Location & Seq
Reverse (-)
4945638 .. 4947785
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG027800.1

Sequence Viewer

Length: 1281 bp
ATGGCATCACCAAGAACCTCCTTTTTCTCAACGTGGAGGTCGCCATCATCCTCGTCACTGAACCGTAGCACTTCACTTGCATCCCCGGCTACAACTGCACCGGTTCCCTCACCACCCACTTTTGAGACTGGACTGTCGCCATCCACATATGAGCCGACAGTGACACCAGAACCCCAATTTTCACCCATGCCGTTCGATTACGATCAGATCATGGACGACTTTTCACCGGTGAAAATAGTGGACTACCATGAGATGCAAAGAGGCCAAGTGGTCTCTCCATCTCCTTCAAATGAATTAGTTCTCAATGAACCGAAGACACAGCCACAAGAAAATATACAGGAACGACTCGGACAAGTTGTCAAGGGTCTTGTTCAAAAAGAGGTAGATGAGTTGATGAAACAAGCAAAGGCGGACAGTGGCAGAACTAGCGTGGAAGAGACCTCTGAGTTAGCGAAGGACGACGGTAAATTGAAGATAACAGCGGCAGAGAAGTCTCAGGCTGACCATGAGAGCCCTAAAATAACTAACATGGAACCGACCTTCACTACCAGGCCAGGCCGATCGGCCACCAAGCAAGTGGCCCATATTTCCAATGTGTCCTTTCAAATTCCTGATGATCTTAAAGTTGACAATGACACAGTAAACGAGTTTGCTCCTAAGGATGAAGTCCTCAGAGAAATTACTGCTCAGAGTAGGAAAAAGTATAGTTTCTCTGACAGACTCCATGGATGGAAAATGGAGTGTAGGATAAGACTGAATGGTGTCCAACAATTCGATAAGTATTTTACGCATGTAGAATCGAAGATTATGACAAGATCAGTTCCCGAGGTTGTGAGGTTTATCCTTTATGAGGTGAACCCTAAACAAATGAAGGAAGCCAATAACAAACGCAAAGGGACAGATATAGGGGAGCCAGCTGATGAGAGACCTAAAAAAATATTAAGGGATGAATACCTAAACAAGTCATCTGAAGGCTCCAGTGATTCTGAAGAAAAGAGGGAATCGTACGATGAGTTGTTTTTTCGTGTAGCCTGGAGTAATCTGATAAACGACGCTGCAGAGAAGGTGCCGTTATTTAGTGAATGGGAAAAATTCAAGGCTGCAAAAAAGAAGGCCAGAACTAAACCATGCGAACCTGAGGTTGGTACACCCAACTTGGATATTGAAGCTCTAGAGACAGCAAACGCCTTGATGGGCCTAGCTGAGGATGAAAGTTTGGTTACGAGAAAGTCGAAGGCTTGTAATGATAGGTCTGGTTTTGGTGTTTTTATCAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

426

Amino Acids

48.01

Weight (kDa)

5.7

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 1066
AciI CCGC 2 cut(s) 410, 482
AcoI YGGCCR 1 cut(s) 564
AcsI RAATTY 2 cut(s) 606, 1091
AcuI CTGAAG 2 cut(s) 990, 1008
AfaI GTAC 2 cut(s) 1007, 1147
AfiI CCNNNNNNNGG 3 cut(s) 850, 1142, 1204
AgeI ACCGGT 2 cut(s) 100, 226
AgsI TTSAA 6 cut(s) 288, 374, 472, 605, 1096, 1166
AhdI GACNNNNNGTC 1 cut(s) 356
AjnI CCWGG 3 cut(s) 548, 553, 1031
AjuI GAANNNNNNNTTGG 2 cut(s) 1125, 1157
AluBI AGCT 3 cut(s) 917, 1169, 1202
AluI AGCT 3 cut(s) 917, 1169, 1202
Alw26I GTCTC 6 cut(s) 119, 277, 431, 498, 919, 1169
Ama87I CYCGRG 1 cut(s) 824
AoxI GGCC 7 cut(s) 262, 551, 556, 564, 579, 1113, 1195
ApeKI GCWGC 2 cut(s) 1055, 1100
ApoI RAATTY 2 cut(s) 606, 1091
AsiGI ACCGGT 2 cut(s) 100, 226
Asp700I GAANNNNTTC 1 cut(s) 297
AspS9I GGNCC 2 cut(s) 580, 1195
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 5 cut(s) 102, 174, 216, 241, 865
AvaI CYCGRG 1 cut(s) 824
AxyI CCTNAGG 2 cut(s) 657, 1137
BanI GGYRCC 1 cut(s) 1066
BanII GRGCYC 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 320
BbvCI CCTCAGC 1 cut(s) 1203
BbvI GCAGC 2 cut(s) 1042, 1087
BccI CCATC 5 cut(s) 52, 148, 286, 723, 1186
BceAI ACGGC 2 cut(s) 175, 1054
BciT130I CCWGG 3 cut(s) 550, 555, 1033
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 6 cut(s) 119, 277, 431, 498, 919, 1169
BfaI CTAG 3 cut(s) 426, 1172, 1199
BfmI CTRYAG 1 cut(s) 1056
BisI GCNGC 3 cut(s) 483, 1056, 1101
BlsI GCNGC 3 cut(s) 484, 1057, 1102
Bme1390I CCNGG 4 cut(s) 86, 550, 555, 1033
BmeRI GACNNNNNGTC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 824
BmgT120I GGNCC 2 cut(s) 580, 1195
BmiI GGNNCC 5 cut(s) 105, 534, 912, 976, 1068
BmrFI CCNGG 4 cut(s) 86, 550, 555, 1033
BmsI GCATC 3 cut(s) 14, 89, 243
BpiI GAAGAC 1 cut(s) 320
BpmI CTGGAG 2 cut(s) 961, 1054
Bpu10I CCTNAGC 1 cut(s) 1203
BpuMI CCSGG 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 201
BsaI GGTCTC 3 cut(s) 277, 431, 919
BsaJI CCNNGG 3 cut(s) 84, 724, 825
BsaWI WCCGGW 2 cut(s) 100, 226
Bsc4I CCNNNNNNNGG 3 cut(s) 850, 1142, 1204
Bse118I RCCGGY 2 cut(s) 100, 226
Bse1I ACTGG 2 cut(s) 133, 978
Bse21I CCTNAGG 2 cut(s) 657, 1137
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 3 cut(s) 550, 555, 1033
BseDI CCNNGG 3 cut(s) 84, 724, 825
BseGI GGATG 7 cut(s) 47, 80, 140, 667, 734, 952, 1213
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 3 cut(s) 850, 1142, 1204
BseMII CTCAG 6 cut(s) 435, 509, 685, 701, 1128, 1194
BseNI ACTGG 2 cut(s) 133, 978
BseXI GCAGC 2 cut(s) 1042, 1087
BsgI GTGCAG 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 563
BshFI GGCC 7 cut(s) 264, 553, 558, 566, 581, 1115, 1197
BshNI GGYRCC 1 cut(s) 1066
BshTI ACCGGT 2 cut(s) 100, 226
BsiEI CGRYCG 1 cut(s) 563
BsiHKCI CYCGRG 1 cut(s) 824
BsiSI CCGG 3 cut(s) 86, 101, 227
BsiWI CGTACG 1 cut(s) 1005
BslFI GGGAC 1 cut(s) 910
BslI CCNNNNNNNGG 3 cut(s) 850, 1142, 1204
BsmAI GTCTC 6 cut(s) 119, 277, 431, 498, 919, 1169
BsmFI GGGAC 1 cut(s) 910
BsnI GGCC 7 cut(s) 264, 553, 558, 566, 581, 1115, 1197
Bso31I GGTCTC 3 cut(s) 277, 431, 919
BsoBI CYCGRG 1 cut(s) 824
Bsp1286I GDGCHC 1 cut(s) 515
Bsp143I GATC 5 cut(s) 202, 207, 560, 616, 815
Bsp19I CCATGG 1 cut(s) 724
BspACI CCGC 2 cut(s) 410, 482
BspANI GGCC 7 cut(s) 264, 553, 558, 566, 581, 1115, 1197
BspCNI CTCAG 6 cut(s) 436, 508, 684, 700, 1129, 1195
BspLI GGNNCC 5 cut(s) 105, 534, 912, 976, 1068
BspMAI CTGCAG 1 cut(s) 1060
BspT107I GGYRCC 1 cut(s) 1066
BspTNI GGTCTC 3 cut(s) 277, 431, 919
BsrFI RCCGGY 2 cut(s) 100, 226
BsrI ACTGG 2 cut(s) 133, 978
BssAI RCCGGY 2 cut(s) 100, 226
BssECI CCNNGG 3 cut(s) 84, 724, 825
BssMI GATC 5 cut(s) 202, 207, 560, 616, 815
BssT1I CCWWGG 1 cut(s) 724
Bst2UI CCWGG 3 cut(s) 550, 555, 1033
Bst4CI ACNGT 6 cut(s) 65, 135, 160, 416, 464, 640
Bst6I CTCTTC 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 915
BstDEI CTNAG 7 cut(s) 444, 495, 657, 671, 687, 1137, 1203
BstDSI CCRYGG 1 cut(s) 724
BstENI CCTNNNNNAGG 2 cut(s) 848, 1202
BstF5I GGATG 7 cut(s) 47, 80, 140, 667, 734, 952, 1213
BstKTI GATC 5 cut(s) 205, 210, 563, 619, 818
BstMAI GTCTC 6 cut(s) 119, 277, 431, 498, 919, 1169
BstMBI GATC 5 cut(s) 202, 207, 560, 616, 815
BstMCI CGRYCG 1 cut(s) 563
BstMWI GCNNNNNNNGC 3 cut(s) 86, 95, 426
BstNI CCWGG 3 cut(s) 550, 555, 1033
BstNSI RCATGY 1 cut(s) 794
BstSCI CCNGG 4 cut(s) 84, 548, 553, 1031
BstSFI CTRYAG 1 cut(s) 1056
BstV1I GCAGC 2 cut(s) 1042, 1087
BstV2I GAAGAC 1 cut(s) 320
BstXI CCANNNNNNTGG 1 cut(s) 577
Bsu36I CCTNAGG 2 cut(s) 657, 1137
BsuRI GGCC 7 cut(s) 264, 553, 558, 566, 581, 1115, 1197
BtgI CCRYGG 1 cut(s) 724
BtsCI GGATG 7 cut(s) 47, 80, 140, 667, 734, 952, 1213
BtsIMutI CAGTG 4 cut(s) 56, 165, 421, 985
Cac8I GCNNGC 1 cut(s) 915
Cfr10I RCCGGY 2 cut(s) 100, 226
Cfr13I GGNCC 2 cut(s) 580, 1195
CseI GACGC 1 cut(s) 1061
Csp6I GTAC 2 cut(s) 1006, 1146
CspAI ACCGGT 2 cut(s) 100, 226
CviAII CATG 8 cut(s) 187, 211, 248, 506, 529, 725, 791, 1128
CviQI GTAC 2 cut(s) 1006, 1146
DdeI CTNAG 7 cut(s) 444, 495, 657, 671, 687, 1137, 1203
DpnI GATC 5 cut(s) 204, 209, 562, 618, 817
DpnII GATC 5 cut(s) 202, 207, 560, 616, 815
DrdI GACNNNNNNGTC 1 cut(s) 133
DriI GACNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 1 cut(s) 564
Eam1104I CTCTTC 1 cut(s) 429
Eam1105I GACNNNNNGTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 429
EciI GGCGGA 1 cut(s) 425
Eco130I CCWWGG 1 cut(s) 724
Eco24I GRGCYC 1 cut(s) 515
Eco31I GGTCTC 3 cut(s) 277, 431, 919
Eco57I CTGAAG 2 cut(s) 990, 1008
Eco81I CCTNAGG 2 cut(s) 657, 1137
Eco88I CYCGRG 1 cut(s) 824
EcoNI CCTNNNNNAGG 2 cut(s) 848, 1202
EcoRII CCWGG 3 cut(s) 548, 553, 1031
EcoT14I CCWWGG 1 cut(s) 724
EcoT38I GRGCYC 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 724
FaeI CATG 8 cut(s) 190, 214, 251, 509, 532, 728, 794, 1131
FaqI GGGAC 1 cut(s) 910
FatI CATG 8 cut(s) 186, 210, 247, 505, 528, 724, 790, 1127
FauNDI CATATG 1 cut(s) 148
Fnu4HI GCNGC 3 cut(s) 483, 1056, 1101
FokI GGATG 7 cut(s) 34, 67, 127, 674, 741, 959, 1220
FriOI GRGCYC 1 cut(s) 515
Fsp4HI GCNGC 3 cut(s) 483, 1056, 1101
FspBI CTAG 3 cut(s) 426, 1172, 1199
GluI GCNGC 3 cut(s) 483, 1056, 1101
GsuI CTGGAG 2 cut(s) 961, 1054
HaeIII GGCC 7 cut(s) 264, 553, 558, 566, 581, 1115, 1197
HapII CCGG 3 cut(s) 86, 101, 227
HgaI GACGC 1 cut(s) 1061
Hin1II CATG 8 cut(s) 190, 214, 251, 509, 532, 728, 794, 1131
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HinfI GANTC 5 cut(s) 345, 720, 797, 983, 1001
HpaII CCGG 3 cut(s) 86, 101, 227
HphI GGTGA 5 cut(s) 102, 174, 216, 241, 865
Hpy166II GTNNAC 5 cut(s) 241, 628, 643, 856, 1148
Hpy188I TCNGA 9 cut(s) 207, 350, 445, 674, 690, 715, 970, 988, 1044
Hpy188III TCNNGA 4 cut(s) 611, 824, 1172, 1273
Hpy8I GTNNAC 5 cut(s) 241, 628, 643, 856, 1148
Hpy99I CGWCG 2 cut(s) 464, 1055
HpyAV CCTTC 8 cut(s) 294, 448, 550, 865, 965, 1057, 1105, 1228
HpyCH4III ACNGT 6 cut(s) 65, 135, 160, 416, 464, 640
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 5 cut(s) 80, 98, 256, 1058, 1103
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 95, 426
HpyF3I CTNAG 7 cut(s) 444, 495, 657, 671, 687, 1137, 1203
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 8 cut(s) 190, 214, 251, 509, 532, 728, 794, 1131
Kzo9I GATC 5 cut(s) 202, 207, 560, 616, 815
LmnI GCTCC 3 cut(s) 658, 910, 980
Lsp1109I GCAGC 2 cut(s) 1042, 1087
LweI GCATC 3 cut(s) 14, 89, 243
MaeI CTAG 3 cut(s) 426, 1172, 1199
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 54, 160, 1219
MalI GATC 5 cut(s) 204, 209, 562, 618, 817
MboI GATC 5 cut(s) 202, 207, 560, 616, 815
MboII GAAGA 5 cut(s) 325, 446, 484, 814, 1001
MhlI GDGCHC 1 cut(s) 515
MluCI AATT 7 cut(s) 176, 293, 467, 606, 678, 770, 1091
MlyI GAGTC 2 cut(s) 339, 714
MmeI TCCRAC 1 cut(s) 790
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 2 cut(s) 621, 941
MspA1I CMGCKG 2 cut(s) 482, 917
MspI CCGG 3 cut(s) 86, 101, 227
MspR9I CCNGG 4 cut(s) 86, 550, 555, 1033
MvaI CCWGG 3 cut(s) 550, 555, 1033
MwoI GCNNNNNNNGC 3 cut(s) 86, 95, 426
NciI CCSGG 1 cut(s) 86
NcoI CCATGG 1 cut(s) 724
NdeI CATATG 1 cut(s) 148
NdeII GATC 5 cut(s) 202, 207, 560, 616, 815
NlaIII CATG 8 cut(s) 190, 214, 251, 509, 532, 728, 794, 1131
NlaIV GGNNCC 5 cut(s) 105, 534, 912, 976, 1068
NmuCI GTSAC 2 cut(s) 54, 160
NspI RCATGY 1 cut(s) 794
PcsI WCGNNNNNNNCGW 1 cut(s) 1229
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 3 cut(s) 797, 983, 1001
Pfl23II CGTACG 1 cut(s) 1005
PinAI ACCGGT 2 cut(s) 100, 226
PkrI GCNGC 3 cut(s) 484, 1057, 1102
Ple19I CGATCG 1 cut(s) 563
PleI GAGTC 2 cut(s) 339, 714
PpsI GAGTC 2 cut(s) 339, 714
Psp6I CCWGG 3 cut(s) 548, 553, 1031
PspGI CCWGG 3 cut(s) 548, 553, 1031
PspLI CGTACG 1 cut(s) 1005
PspN4I GGNNCC 5 cut(s) 105, 534, 912, 976, 1068
PspPI GGNCC 2 cut(s) 580, 1195
PstI CTGCAG 1 cut(s) 1060
PvuI CGATCG 1 cut(s) 563
PvuII CAGCTG 1 cut(s) 917
RsaI GTAC 2 cut(s) 1007, 1147
RsaNI GTAC 2 cut(s) 1006, 1146
SaqAI TTAA 2 cut(s) 621, 941
SatI GCNGC 3 cut(s) 483, 1056, 1101
Sau3AI GATC 5 cut(s) 202, 207, 560, 616, 815
Sau96I GGNCC 2 cut(s) 580, 1195
SchI GAGTC 2 cut(s) 339, 714
ScrFI CCNGG 4 cut(s) 86, 550, 555, 1033
SduI GDGCHC 1 cut(s) 515
SfaNI GCATC 3 cut(s) 14, 89, 243
SfcI CTRYAG 1 cut(s) 1056
SgrAI CRCCGGYG 1 cut(s) 226
Sse9I AATT 7 cut(s) 176, 293, 467, 606, 678, 770, 1091
SsiI CCGC 2 cut(s) 410, 482
SspI AATATT 1 cut(s) 939
SspMI CTAG 3 cut(s) 426, 1172, 1199
StyD4I CCNGG 4 cut(s) 84, 548, 553, 1031
StyI CCWWGG 1 cut(s) 724
TaaI ACNGT 6 cut(s) 65, 135, 160, 416, 464, 640
TaiI ACGT 1 cut(s) 35
TaqI TCGA 4 cut(s) 195, 774, 800, 1232
TasI AATT 7 cut(s) 176, 293, 467, 606, 678, 770, 1091
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 3 cut(s) 797, 983, 1001
Tru1I TTAA 2 cut(s) 621, 941
Tru9I TTAA 2 cut(s) 621, 941
TscAI CASTG 4 cut(s) 63, 165, 421, 985
TseFI GTSAC 2 cut(s) 54, 160
TseI GCWGC 2 cut(s) 1055, 1100
Tsp45I GTSAC 2 cut(s) 54, 160
TspDTI ATGAA 7 cut(s) 306, 321, 410, 678, 884, 963, 1224
TspRI CASTG 4 cut(s) 63, 165, 421, 985
XagI CCTNNNNNAGG 2 cut(s) 848, 1202
XapI RAATTY 2 cut(s) 606, 1091
XbaI TCTAGA 1 cut(s) 1171
XceI RCATGY 1 cut(s) 794
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 3 cut(s) 426, 1172, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.