Rorug03G0330600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001250
Physical Location & Seq
Reverse (-)
38379808 .. 38381523
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0330600.1

Sequence Viewer

Length: 1716 bp
ATGCAAAGGAAACGTTTGAAGAATGGTGGTGACATCAGCTCCGACTTGGAGGCTCTCCGGATGGAGGATCTTGATTCTTCGATTCATCCTTCACCTACTCTGCAACCTCAGATGACCTATGTCGGGACTCTCAAAAACCCGGTCGATCGTTACCGGCAGACTATGACTGAAGACTTCGAATTTACTGACGAAGACTGCTCCTACTCCCTGGGTAAGCATGGACAGAATGTTACTTTCTCTGATAAGGTTCATAATAAGCTTGACTATGATTGGCGTTGTGCTGTGATCGTTAAACTCATGTGGAAACCTAACTCCACGAACACTTTTGATTTTATGCTAAGAGGATTAAGAAGGAAATGGCAAGTGAAAGGGAGGTGGCAACTCATCGATTTACCTAATGATTTCTTCATTGTCAAGTTTAATCTGGAGGAAGATATGAATTATGCTCTCTGTGGAGGTCCTTGGATCCTTGCTGGGCAGACTCTGATTGTTAGAAAATGGAGACCTGATTTTGATCCCATGAATGAAGTGCTTGGAAAGATGGCTGTGTGGGTCAGAATTTTTGGTTTGCCTGTCAAATTTTTCAAGGACTATACTGTGGCTAAAATTGGGAAAATCCTGGGTGATATAGTCAAAGTGGACAAACTCACAATTGGTCAGGCTAGAGGGCAGTTTGCTCGTATTTGCATTGAGGTTGATCTATCTAAGCCTCTACATCCTTTTGTTGAAGTGGAGTCCATTGCTTATAATGTGGTTTATGAAGGCATCTCTCTCATATGCTTTGAGTGTGGCTGCTTTGGCCACTCAAAAGACAAGTGTCCCTCAATTAAGAATGATACTGGTGAAGCTGACAAAAATCTGAACACTTTTAATGCTACCGCTCTATCTCATTCCTCTAGTGATGAGACATCTTCTCCACCTGAGATGAATGTTTCTACAATGCTGCAAGATGTGAATGCTCAGAGACAAGGTGACATTATTAAAGAAGACATGGGACCTTGGACGTTGATGAGTTATAGGAATAAAAAGAAGAATGGAGAGTCTGGTGGAGCTAGGAAAAATAGTAATGGCAGTGGTTCCAGATTTGCTGTTTTGCAGGAGGAAAGTGGCAAGGACTCTGAGTTGCCTGAGCAGACCATTGAGAATAACTCTTCTGATAGTAGCCCTCCCATTGTGAAACTTTGGAAAAGCCTTCAAGTGAAGAAGAAGAAGACAGTTCCTATGAACAATGAGCCTACCAAAACCAAGATTGCTGCTACTACCCATACCTCTGCCAGGAAATTTGGCCCCTCTGGTAACCATTCTTCCTCTGTGAAAGTTAATGTTCACAATGATAAGACTATATCTCTGAAGACTAAAGGTAGCTCTAGAATCCCTATGAAAGATGTGTCTAATGTTGAAAATTCTAGTGGCTCTAGAACTGATCTTCAGTACCAAAGAAAATCCAAAAGTGTGGCTGTTTCTGGTCCTAAGCATAATTCTCACATTTTCAAAAAGCTCTCCTTTGAAAATATGGGAACCAATGCTTTAGGAGATGGAATTGCGGCCATCTTTGGGCATTGTCCTCCTGAGGAGGCCATTGGGGACAAGGCTAGCACCTCATCTCTTGATATGGACTCTGAAACATTTGTGGAAAAGACCTTTACTGCTAATAGCATGATGACCAATGATGATAACCTACCTTCCCAACATGGGGAGGGTATGGTTGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

571

Amino Acids

63.69

Weight (kDa)

8.19

Isoelectric Point (pI)

35.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 90 - 174 3.9e-13 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AccBSI CCGCTC 1 cut(s) 881
AccIII TCCGGA 1 cut(s) 57
AciI CCGC 2 cut(s) 879, 1544
AclI AACGTT 1 cut(s) 13
AclWI GGATC 4 cut(s) 75, 460, 473, 509
AcoI YGGCCR 2 cut(s) 799, 1545
AcsI RAATTY 5 cut(s) 179, 558, 578, 1280, 1402
AcuI CTGAAG 3 cut(s) 189, 1370, 1412
AfaI GTAC 1 cut(s) 1433
AfiI CCNNNNNNNGG 6 cut(s) 64, 123, 1275, 1554, 1692, 1693
AgsI TTSAA 7 cut(s) 19, 586, 728, 1196, 1400, 1492, 1508
AjnI CCWGG 3 cut(s) 207, 618, 1274
AluBI AGCT 6 cut(s) 39, 259, 848, 1052, 1365, 1498
AluI AGCT 6 cut(s) 39, 259, 848, 1052, 1365, 1498
Alw26I GTCTC 3 cut(s) 496, 899, 958
AlwI GGATC 4 cut(s) 75, 460, 473, 509
AlwNI CAGNNNCTG 1 cut(s) 484
Aor13HI TCCGGA 1 cut(s) 57
AoxI GGCC 4 cut(s) 799, 1285, 1545, 1575
ApeKI GCWGC 3 cut(s) 792, 943, 1253
ApoI RAATTY 5 cut(s) 179, 558, 578, 1280, 1402
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
AspS9I GGNCC 4 cut(s) 458, 995, 1286, 1466
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 5 cut(s) 41, 84, 635, 854, 983
AsuII TTCGAA 1 cut(s) 177
AsuNHI GCTAGC 1 cut(s) 1592
AvaII GGWCC 3 cut(s) 458, 995, 1466
AxyI CCTNAGG 1 cut(s) 1569
BalI TGGCCA 1 cut(s) 801
BamHI GGATCC 1 cut(s) 465
BarI GAAGNNNNNNTAC 2 cut(s) 1288, 1320
BbsI GAAGAC 5 cut(s) 177, 198, 993, 1217, 1358
BbvI GCAGC 3 cut(s) 779, 930, 1240
BccI CCATC 4 cut(s) 55, 535, 1529, 1556
BcgI CGANNNNNNTGC 2 cut(s) 659, 693
BciT130I CCWGG 3 cut(s) 209, 620, 1276
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 3 cut(s) 496, 899, 958
BfaI CTAG 7 cut(s) 663, 897, 1053, 1368, 1407, 1416, 1593
BisI GCNGC 4 cut(s) 793, 944, 1254, 1545
BlsI GCNGC 4 cut(s) 794, 945, 1255, 1546
Bme1390I CCNGG 4 cut(s) 140, 209, 620, 1276
Bme18I GGWCC 3 cut(s) 458, 995, 1466
BmgT120I GGNCC 4 cut(s) 458, 995, 1286, 1466
BmiI GGNNCC 5 cut(s) 467, 996, 1078, 1288, 1519
BmrFI CCNGG 4 cut(s) 140, 209, 620, 1276
BmsI GCATC 2 cut(s) 774, 1699
BmtI GCTAGC 1 cut(s) 1596
BoxI GACNNNNGTC 2 cut(s) 119, 816
BpiI GAAGAC 5 cut(s) 177, 198, 993, 1217, 1358
BplI GAGNNNNNCTC 2 cut(s) 1133, 1165
BpmI CTGGAG 1 cut(s) 446
Bpu10I CCTNAGC 2 cut(s) 1128, 1470
Bpu14I TTCGAA 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 140
Bsa29I ATCGAT 1 cut(s) 387
BsaBI GATNNNNATC 1 cut(s) 513
BsaI GGTCTC 1 cut(s) 496
BsaJI CCNNGG 5 cut(s) 207, 208, 461, 619, 998
BsaWI WCCGGW 1 cut(s) 57
BsaXI ACNNNNNCTCC 4 cut(s) 23, 53, 898, 928
Bsc4I CCNNNNNNNGG 6 cut(s) 64, 123, 1275, 1554, 1692, 1693
Bse118I RCCGGY 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 844
Bse21I CCTNAGG 1 cut(s) 1569
Bse3DI GCAATG 1 cut(s) 738
Bse8I GATNNNNATC 1 cut(s) 513
BseAI TCCGGA 1 cut(s) 57
BseBI CCWGG 3 cut(s) 209, 620, 1276
BseCI ATCGAT 1 cut(s) 387
BseDI CCNNGG 5 cut(s) 207, 208, 461, 619, 998
BseGI GGATG 3 cut(s) 66, 85, 715
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 6 cut(s) 64, 123, 1275, 1554, 1692, 1693
BseMI GCAATG 1 cut(s) 738
BseMII CTCAG 6 cut(s) 122, 912, 974, 1110, 1119, 1560
BseNI ACTGG 1 cut(s) 844
BseRI GAGGAG 1 cut(s) 1586
BseXI GCAGC 3 cut(s) 779, 930, 1240
BseYI CCCAGC 1 cut(s) 473
Bsh1285I CGRYCG 2 cut(s) 144, 148
BshFI GGCC 4 cut(s) 801, 1287, 1547, 1577
BshVI ATCGAT 1 cut(s) 387
BsiEI CGRYCG 2 cut(s) 144, 148
BsiSI CCGG 3 cut(s) 58, 140, 154
BslFI GGGAC 4 cut(s) 139, 804, 1008, 1598
BslI CCNNNNNNNGG 6 cut(s) 64, 123, 1275, 1554, 1692, 1693
BsmAI GTCTC 3 cut(s) 496, 899, 958
BsmFI GGGAC 4 cut(s) 139, 804, 1008, 1598
BsmI GAATGC 1 cut(s) 961
BsnI GGCC 4 cut(s) 801, 1287, 1547, 1577
Bso31I GGTCTC 1 cut(s) 496
Bsp119I TTCGAA 1 cut(s) 177
Bsp13I TCCGGA 1 cut(s) 57
Bsp143I GATC 7 cut(s) 67, 145, 285, 465, 514, 697, 1423
BspACI CCGC 2 cut(s) 879, 1544
BspANI GGCC 4 cut(s) 801, 1287, 1547, 1577
BspCNI CTCAG 6 cut(s) 121, 913, 973, 1111, 1120, 1561
BspDI ATCGAT 1 cut(s) 387
BspEI TCCGGA 1 cut(s) 57
BspLI GGNNCC 5 cut(s) 467, 996, 1078, 1288, 1519
BspOI GCTAGC 1 cut(s) 1596
BspPI GGATC 4 cut(s) 75, 460, 473, 509
BspT104I TTCGAA 1 cut(s) 177
BspTNI GGTCTC 1 cut(s) 496
BsrBI CCGCTC 1 cut(s) 881
BsrDI GCAATG 1 cut(s) 738
BsrFI RCCGGY 1 cut(s) 153
BsrI ACTGG 1 cut(s) 844
BssAI RCCGGY 1 cut(s) 153
BssECI CCNNGG 5 cut(s) 207, 208, 461, 619, 998
BssMI GATC 7 cut(s) 67, 145, 285, 465, 514, 697, 1423
BssT1I CCWWGG 2 cut(s) 461, 998
Bst2UI CCWGG 3 cut(s) 209, 620, 1276
Bst4CI ACNGT 2 cut(s) 598, 1216
Bst6I CTCTTC 1 cut(s) 1156
BstBI TTCGAA 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 1594
BstDEI CTNAG 9 cut(s) 108, 338, 705, 921, 960, 1119, 1128, 1470, 1569
BstEII GGTNACC 1 cut(s) 1295
BstENI CCTNNNNNAGG 1 cut(s) 1273
BstF5I GGATG 3 cut(s) 66, 85, 715
BstKTI GATC 7 cut(s) 70, 148, 288, 468, 517, 700, 1426
BstMAI GTCTC 3 cut(s) 496, 899, 958
BstMBI GATC 7 cut(s) 67, 145, 285, 465, 514, 697, 1423
BstMCI CGRYCG 2 cut(s) 144, 148
BstMWI GCNNNNNNNGC 1 cut(s) 798
BstNI CCWGG 3 cut(s) 209, 620, 1276
BstPAI GACNNNNGTC 2 cut(s) 119, 816
BstPI GGTNACC 1 cut(s) 1295
BstSCI CCNGG 4 cut(s) 138, 207, 618, 1274
BstV1I GCAGC 3 cut(s) 779, 930, 1240
BstV2I GAAGAC 5 cut(s) 177, 198, 993, 1217, 1358
BstX2I RGATCY 2 cut(s) 67, 465
BstXI CCANNNNNNTGG 1 cut(s) 1453
BstYI RGATCY 2 cut(s) 67, 465
Bsu15I ATCGAT 1 cut(s) 387
Bsu36I CCTNAGG 1 cut(s) 1569
BsuRI GGCC 4 cut(s) 801, 1287, 1547, 1577
BsuTUI ATCGAT 1 cut(s) 387
BtsCI GGATG 3 cut(s) 66, 85, 715
BtsI GCAGTG 1 cut(s) 1078
BtsIMutI CAGTG 1 cut(s) 1078
Cac8I GCNNGC 1 cut(s) 1594
CaiI CAGNNNCTG 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 153
Cfr13I GGNCC 4 cut(s) 458, 995, 1286, 1466
ClaI ATCGAT 1 cut(s) 387
Csp6I GTAC 1 cut(s) 1432
CviAII CATG 6 cut(s) 218, 298, 520, 991, 1657, 1691
CviQI GTAC 1 cut(s) 1432
DdeI CTNAG 9 cut(s) 108, 338, 705, 921, 960, 1119, 1128, 1470, 1569
DpnI GATC 7 cut(s) 69, 147, 287, 467, 516, 699, 1425
DpnII GATC 7 cut(s) 67, 145, 285, 465, 514, 697, 1423
EaeI YGGCCR 2 cut(s) 799, 1545
Eam1104I CTCTTC 1 cut(s) 1156
EarI CTCTTC 1 cut(s) 1156
Eco130I CCWWGG 2 cut(s) 461, 998
Eco31I GGTCTC 1 cut(s) 496
Eco47I GGWCC 3 cut(s) 458, 995, 1466
Eco57I CTGAAG 3 cut(s) 189, 1370, 1412
Eco81I CCTNAGG 1 cut(s) 1569
Eco91I GGTNACC 1 cut(s) 1295
EcoNI CCTNNNNNAGG 1 cut(s) 1273
EcoO109I RGGNCCY 2 cut(s) 458, 995
EcoO65I GGTNACC 1 cut(s) 1295
EcoRII CCWGG 3 cut(s) 207, 618, 1274
EcoT14I CCWWGG 2 cut(s) 461, 998
ErhI CCWWGG 2 cut(s) 461, 998
FaeI CATG 6 cut(s) 221, 301, 523, 994, 1660, 1694
FalI AAGNNNNNCTT 2 cut(s) 1487, 1519
FaqI GGGAC 4 cut(s) 139, 804, 1008, 1598
FatI CATG 6 cut(s) 217, 297, 519, 990, 1656, 1690
FauNDI CATATG 1 cut(s) 776
Fnu4HI GCNGC 4 cut(s) 793, 944, 1254, 1545
FokI GGATG 3 cut(s) 72, 73, 702
Fsp4HI GCNGC 4 cut(s) 793, 944, 1254, 1545
FspBI CTAG 7 cut(s) 663, 897, 1053, 1368, 1407, 1416, 1593
GluI GCNGC 4 cut(s) 793, 944, 1254, 1545
GsaI CCCAGC 1 cut(s) 477
GsuI CTGGAG 1 cut(s) 446
HaeIII GGCC 4 cut(s) 801, 1287, 1547, 1577
HapII CCGG 3 cut(s) 58, 140, 154
Hin1II CATG 6 cut(s) 221, 301, 523, 994, 1660, 1694
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 9 cut(s) 74, 82, 127, 481, 734, 1040, 1115, 1371, 1616
HpaII CCGG 3 cut(s) 58, 140, 154
HphI GGTGA 5 cut(s) 41, 84, 635, 854, 983
Hpy166II GTNNAC 2 cut(s) 640, 1327
Hpy188III TCNNGA 9 cut(s) 58, 71, 124, 425, 1080, 1368, 1416, 1568, 1607
Hpy8I GTNNAC 2 cut(s) 640, 1327
HpyAV CCTTC 5 cut(s) 99, 345, 755, 1202, 1692
HpyCH4III ACNGT 2 cut(s) 598, 1216
HpyCH4IV ACGT 2 cut(s) 13, 1004
HpyCH4V TGCA 5 cut(s) 4, 103, 687, 946, 1096
HpyF10VI GCNNNNNNNGC 1 cut(s) 798
HpyF3I CTNAG 9 cut(s) 108, 338, 705, 921, 960, 1119, 1128, 1470, 1569
HpySE526I ACGT 2 cut(s) 13, 1004
Hsp92II CATG 6 cut(s) 221, 301, 523, 994, 1660, 1694
Kpn2I TCCGGA 1 cut(s) 57
Kzo9I GATC 7 cut(s) 67, 145, 285, 465, 514, 697, 1423
LmnI GCTCC 3 cut(s) 44, 203, 1049
Lsp1109I GCAGC 3 cut(s) 779, 930, 1240
LweI GCATC 2 cut(s) 774, 1699
MaeI CTAG 7 cut(s) 663, 897, 1053, 1368, 1407, 1416, 1593
MaeII ACGT 2 cut(s) 13, 1004
MaeIII GTNAC 5 cut(s) 29, 149, 229, 971, 1295
MalI GATC 7 cut(s) 69, 147, 287, 467, 516, 699, 1425
MbiI CCGCTC 1 cut(s) 881
MboI GATC 7 cut(s) 67, 145, 285, 465, 514, 697, 1423
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 2 cut(s) 67, 465
MlsI TGGCCA 1 cut(s) 801
MluNI TGGCCA 1 cut(s) 801
MlyI GAGTC 6 cut(s) 121, 475, 743, 1049, 1109, 1610
MmeI TCCRAC 1 cut(s) 66
Mox20I TGGCCA 1 cut(s) 801
MroI TCCGGA 1 cut(s) 57
MscI TGGCCA 1 cut(s) 801
MseI TTAA 7 cut(s) 291, 347, 420, 828, 870, 981, 1320
Msp20I TGGCCA 1 cut(s) 801
MspI CCGG 3 cut(s) 58, 140, 154
MspR9I CCNGG 4 cut(s) 140, 209, 620, 1276
MunI CAATTG 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 961
MvaI CCWGG 3 cut(s) 209, 620, 1276
MwoI GCNNNNNNNGC 1 cut(s) 798
NciI CCSGG 1 cut(s) 140
NdeI CATATG 1 cut(s) 776
NdeII GATC 7 cut(s) 67, 145, 285, 465, 514, 697, 1423
NheI GCTAGC 1 cut(s) 1592
NlaIII CATG 6 cut(s) 221, 301, 523, 994, 1660, 1694
NlaIV GGNNCC 5 cut(s) 467, 996, 1078, 1288, 1519
NmuCI GTSAC 2 cut(s) 29, 971
NspV TTCGAA 1 cut(s) 177
PasI CCCWGGG 1 cut(s) 208
PctI GAATGC 1 cut(s) 961
PfeI GAWTC 3 cut(s) 74, 82, 1371
PkrI GCNGC 4 cut(s) 794, 945, 1255, 1546
Ple19I CGATCG 1 cut(s) 148
PleI GAGTC 6 cut(s) 121, 475, 742, 1048, 1109, 1610
PpsI GAGTC 6 cut(s) 121, 475, 742, 1048, 1109, 1610
PpuMI RGGWCCY 2 cut(s) 458, 995
PshAI GACNNNNGTC 2 cut(s) 119, 816
PsiI TTATAA 1 cut(s) 747
Psp1406I AACGTT 1 cut(s) 13
Psp5II RGGWCCY 2 cut(s) 458, 995
Psp6I CCWGG 3 cut(s) 207, 618, 1274
PspEI GGTNACC 1 cut(s) 1295
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 3 cut(s) 207, 618, 1274
PspN4I GGNNCC 5 cut(s) 467, 996, 1078, 1288, 1519
PspPI GGNCC 4 cut(s) 458, 995, 1286, 1466
PspPPI RGGWCCY 2 cut(s) 458, 995
PstNI CAGNNNCTG 1 cut(s) 484
PsuI RGATCY 2 cut(s) 67, 465
PvuI CGATCG 1 cut(s) 148
RsaI GTAC 1 cut(s) 1433
RsaNI GTAC 1 cut(s) 1432
SaqAI TTAA 7 cut(s) 291, 347, 420, 828, 870, 981, 1320
SatI GCNGC 4 cut(s) 793, 944, 1254, 1545
Sau3AI GATC 7 cut(s) 67, 145, 285, 465, 514, 697, 1423
Sau96I GGNCC 4 cut(s) 458, 995, 1286, 1466
SchI GAGTC 6 cut(s) 121, 475, 743, 1049, 1109, 1610
ScrFI CCNGG 4 cut(s) 140, 209, 620, 1276
SfaNI GCATC 2 cut(s) 774, 1699
SfuI TTCGAA 1 cut(s) 177
SinI GGWCC 3 cut(s) 458, 995, 1466
SsiI CCGC 2 cut(s) 879, 1544
SspMI CTAG 7 cut(s) 663, 897, 1053, 1368, 1407, 1416, 1593
StyD4I CCNGG 4 cut(s) 138, 207, 618, 1274
StyI CCWWGG 2 cut(s) 461, 998
TaaI ACNGT 2 cut(s) 598, 1216
TaiI ACGT 2 cut(s) 16, 1007
TaqI TCGA 4 cut(s) 80, 144, 177, 387
TauI GCSGC 1 cut(s) 1547
TfiI GAWTC 3 cut(s) 74, 82, 1371
Tru1I TTAA 7 cut(s) 291, 347, 420, 828, 870, 981, 1320
Tru9I TTAA 7 cut(s) 291, 347, 420, 828, 870, 981, 1320
TscAI CASTG 1 cut(s) 1078
TseFI GTSAC 2 cut(s) 29, 971
TseI GCWGC 3 cut(s) 792, 943, 1253
Tsp45I GTSAC 2 cut(s) 29, 971
TspRI CASTG 1 cut(s) 1078
VpaK11BI GGWCC 3 cut(s) 458, 995, 1466
XagI CCTNNNNNAGG 1 cut(s) 1273
XapI RAATTY 5 cut(s) 179, 558, 578, 1280, 1402
XbaI TCTAGA 2 cut(s) 1367, 1415
XspI CTAG 7 cut(s) 663, 897, 1053, 1368, 1407, 1416, 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.