Rmu_sc0006160.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006160.1
OG0001250
Physical Location & Seq
Reverse (-)
13563 .. 16355
2793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006160.1_g000004.1.cds

Sequence Viewer

Length: 1458 bp
atggcatcaccaagatcctcctttttctcaacgtggaggtcgccatcgtccccgtcactgaaccgtagcacttcacttgcatccccggctacaactgcaccggttccctcaccacccatttttgagactggactgtcgccatccacatataagccgacagtgacaccagaaccccaattttcacccatgccgttcgattacgatcagatcatggacgacttttcaccggtgaaaatagtggactaccatgagatgcaaagaggccaagtggtctctccatctccttcaaatgaattagttctcaatgaaccgaagacacagccacaagaaaatatacaggaacgactcggacaagttgtcaagggacttgttcaaaaagaggtagataacttgatgaaacaagcaaagtcggacagtggcagaactagcgtgaaagaggtagataagttgatgaaacaagcaaagtcggacagtggcagaactagcgtggaagagaccactgagttagcgaaggacgacggtaaattaaagataacagcggcagagaaggctcaggctgaccatgagagccctaaaataactgacatggaaccgaccttcactaccaggccaggccgatcggccaccaagcaagtggcccatatttccaatgtgtcctttcaaattcctggtgatcttaaagttgacaatgacacagtcaacgaggaaccgaccttcactaccaggccaggccgatcggccaccaagcaagtggcccatatttccaatgtgtcctttcaaattcctggtgatcttaaagttgacaatgacacagtaaacgagtttgctcctaaggatgaagtcctcagagaaattactgctcagagtaggaaaaagtatagtttctctgacagactccatggatggaaaatggagtgtaggataagactgaatggtatccaacaattcgataagtattttacgcatgtagaatcgaagattgtgacaagatcagttcccgaggttgtgaggtttattctttatgaggtgaaccctaaacaaatgaaggaagccaatagcaaacgcaaagggatagatataggggagccagctgatgagagacctaaaaaaatattaaaggatgaatacctaagcaagtcatctgaaggcgctgccagtgattctgaagaaaagagggaatcgtacgatgagttgttttttcgcgtagcctggagtaatctgataaacgacgctgcagagaaggtgccgttatttagtgaatgggaaaaattcaaggctgcaaaaaagaaggccagaactaaactatgcgaacctgaggttggtacacccaacttggatattgaagctctagagacagcaaacgccttgatgggactagctgaggatgaaagtttggctacgagaaagtcgaaggcttgtaatgataggtctcgttttggtgtttttgtcaggaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

485

Amino Acids

54.34

Weight (kDa)

6.67

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 1243
AccII CGCG 1 cut(s) 1203
AciI CCGC 1 cut(s) 539
AclWI GGATC 1 cut(s) 9
AcoI YGGCCR 2 cut(s) 621, 738
AcsI RAATTY 3 cut(s) 663, 780, 1268
AcuI CTGAAG 2 cut(s) 1164, 1185
AfaI GTAC 2 cut(s) 1184, 1324
AfiI CCNNNNNNNGG 1 cut(s) 1319
AgeI ACCGGT 2 cut(s) 100, 226
AgsI TTSAA 6 cut(s) 288, 374, 662, 779, 1273, 1343
AhdI GACNNNNNGTC 1 cut(s) 356
AjnI CCWGG 7 cut(s) 605, 610, 667, 722, 727, 784, 1208
AjuI GAANNNNNNNTTGG 2 cut(s) 1302, 1334
AluBI AGCT 3 cut(s) 1091, 1346, 1379
AluI AGCT 3 cut(s) 1091, 1346, 1379
Alw26I GTCTC 6 cut(s) 119, 277, 488, 1093, 1346, 1434
AlwI GGATC 1 cut(s) 9
Ama87I CYCGRG 1 cut(s) 998
ApeKI GCWGC 3 cut(s) 1151, 1232, 1277
ApoI RAATTY 3 cut(s) 663, 780, 1268
AsiGI ACCGGT 2 cut(s) 100, 226
Asp700I GAANNNNTTC 1 cut(s) 297
AspLEI GCGC 1 cut(s) 1151
AspS9I GGNCC 2 cut(s) 637, 754
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 7 cut(s) 102, 174, 216, 241, 683, 800, 1039
AvaI CYCGRG 1 cut(s) 998
AxyI CCTNAGG 2 cut(s) 831, 1314
BanI GGYRCC 1 cut(s) 1243
BanII GRGCYC 1 cut(s) 572
BbsI GAAGAC 1 cut(s) 320
BbvCI CCTCAGC 1 cut(s) 1380
BbvI GCAGC 3 cut(s) 1138, 1219, 1264
BccI CCATC 5 cut(s) 52, 148, 286, 897, 1363
BceAI ACGGC 2 cut(s) 175, 1231
BciT130I CCWGG 7 cut(s) 607, 612, 669, 724, 729, 786, 1210
BciVI GTATCC 1 cut(s) 947
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 6 cut(s) 119, 277, 488, 1093, 1346, 1434
BfaI CTAG 4 cut(s) 426, 483, 1349, 1376
BfmI CTRYAG 1 cut(s) 1233
BfoI RGCGCY 1 cut(s) 1152
BfuI GTATCC 1 cut(s) 947
BisI GCNGC 4 cut(s) 540, 1152, 1233, 1278
BlsI GCNGC 4 cut(s) 541, 1153, 1234, 1279
Bme1390I CCNGG 8 cut(s) 86, 607, 612, 669, 724, 729, 786, 1210
BmeRI GACNNNNNGTC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 998
BmgT120I GGNCC 2 cut(s) 637, 754
BmiI GGNNCC 5 cut(s) 105, 591, 708, 1086, 1245
BmrFI CCNGG 8 cut(s) 86, 607, 612, 669, 724, 729, 786, 1210
BmsI GCATC 3 cut(s) 14, 89, 243
BpiI GAAGAC 1 cut(s) 320
BpmI CTGGAG 1 cut(s) 1231
Bpu10I CCTNAGC 3 cut(s) 552, 1130, 1380
BpuMI CCSGG 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 201
BsaI GGTCTC 4 cut(s) 277, 488, 1093, 1434
BsaJI CCNNGG 3 cut(s) 84, 898, 999
BsaWI WCCGGW 2 cut(s) 100, 226
Bsc4I CCNNNNNNNGG 1 cut(s) 1319
Bse118I RCCGGY 2 cut(s) 100, 226
Bse1I ACTGG 2 cut(s) 133, 1155
Bse21I CCTNAGG 2 cut(s) 831, 1314
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 7 cut(s) 607, 612, 669, 724, 729, 786, 1210
BseDI CCNNGG 3 cut(s) 84, 898, 999
BseGI GGATG 6 cut(s) 80, 140, 841, 908, 1126, 1390
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 1319
BseMII CTCAG 6 cut(s) 492, 566, 859, 875, 1305, 1371
BseNI ACTGG 2 cut(s) 133, 1155
BseXI GCAGC 3 cut(s) 1138, 1219, 1264
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 1203
Bsh1285I CGRYCG 2 cut(s) 620, 737
BshNI GGYRCC 1 cut(s) 1243
BshTI ACCGGT 2 cut(s) 100, 226
BsiEI CGRYCG 2 cut(s) 620, 737
BsiHKCI CYCGRG 1 cut(s) 998
BsiSI CCGG 3 cut(s) 86, 101, 227
BsiWI CGTACG 1 cut(s) 1182
BslFI GGGAC 3 cut(s) 34, 378, 1386
BslI CCNNNNNNNGG 1 cut(s) 1319
BsmAI GTCTC 6 cut(s) 119, 277, 488, 1093, 1346, 1434
BsmFI GGGAC 3 cut(s) 34, 378, 1386
Bso31I GGTCTC 4 cut(s) 277, 488, 1093, 1434
BsoBI CYCGRG 1 cut(s) 998
Bsp1286I GDGCHC 1 cut(s) 572
Bsp143I GATC 8 cut(s) 14, 202, 207, 617, 673, 734, 790, 989
Bsp19I CCATGG 1 cut(s) 898
BspACI CCGC 1 cut(s) 539
BspCNI CTCAG 6 cut(s) 493, 565, 858, 874, 1306, 1372
BspFNI CGCG 1 cut(s) 1203
BspLI GGNNCC 5 cut(s) 105, 591, 708, 1086, 1245
BspMAI CTGCAG 1 cut(s) 1237
BspPI GGATC 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 1243
BspTNI GGTCTC 4 cut(s) 277, 488, 1093, 1434
BsrFI RCCGGY 2 cut(s) 100, 226
BsrI ACTGG 2 cut(s) 133, 1155
BssAI RCCGGY 2 cut(s) 100, 226
BssECI CCNNGG 3 cut(s) 84, 898, 999
BssMI GATC 8 cut(s) 14, 202, 207, 617, 673, 734, 790, 989
BssT1I CCWWGG 1 cut(s) 898
Bst2UI CCWGG 7 cut(s) 607, 612, 669, 724, 729, 786, 1210
Bst4CI ACNGT 8 cut(s) 65, 135, 160, 416, 473, 521, 697, 814
Bst6I CTCTTC 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 1089
BstDEI CTNAG 8 cut(s) 501, 552, 831, 845, 861, 1130, 1314, 1380
BstDSI CCRYGG 1 cut(s) 898
BstF5I GGATG 6 cut(s) 80, 140, 841, 908, 1126, 1390
BstFNI CGCG 1 cut(s) 1203
BstH2I RGCGCY 1 cut(s) 1152
BstHHI GCGC 1 cut(s) 1151
BstKTI GATC 8 cut(s) 17, 205, 210, 620, 676, 737, 793, 992
BstMAI GTCTC 6 cut(s) 119, 277, 488, 1093, 1346, 1434
BstMBI GATC 8 cut(s) 14, 202, 207, 617, 673, 734, 790, 989
BstMCI CGRYCG 2 cut(s) 620, 737
BstMWI GCNNNNNNNGC 5 cut(s) 86, 95, 426, 483, 548
BstNI CCWGG 7 cut(s) 607, 612, 669, 724, 729, 786, 1210
BstNSI RCATGY 1 cut(s) 968
BstSCI CCNGG 8 cut(s) 84, 605, 610, 667, 722, 727, 784, 1208
BstSFI CTRYAG 1 cut(s) 1233
BstUI CGCG 1 cut(s) 1203
BstV1I GCAGC 3 cut(s) 1138, 1219, 1264
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 14
BstXI CCANNNNNNTGG 2 cut(s) 634, 751
BstYI RGATCY 1 cut(s) 14
Bsu36I CCTNAGG 2 cut(s) 831, 1314
BsuI GTATCC 1 cut(s) 947
BtgI CCRYGG 1 cut(s) 898
BtsCI GGATG 6 cut(s) 80, 140, 841, 908, 1126, 1390
BtsIMutI CAGTG 6 cut(s) 56, 165, 421, 478, 498, 1162
Cac8I GCNNGC 1 cut(s) 1089
CfoI GCGC 1 cut(s) 1151
Cfr10I RCCGGY 2 cut(s) 100, 226
Cfr13I GGNCC 2 cut(s) 637, 754
CseI GACGC 1 cut(s) 1238
Csp6I GTAC 2 cut(s) 1183, 1323
CspAI ACCGGT 2 cut(s) 100, 226
CviAII CATG 7 cut(s) 187, 211, 248, 563, 586, 899, 965
CviQI GTAC 2 cut(s) 1183, 1323
DdeI CTNAG 8 cut(s) 501, 552, 831, 845, 861, 1130, 1314, 1380
DpnI GATC 8 cut(s) 16, 204, 209, 619, 675, 736, 792, 991
DpnII GATC 8 cut(s) 14, 202, 207, 617, 673, 734, 790, 989
DrdI GACNNNNNNGTC 1 cut(s) 133
DriI GACNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 2 cut(s) 621, 738
Eam1104I CTCTTC 1 cut(s) 486
Eam1105I GACNNNNNGTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 898
Eco24I GRGCYC 1 cut(s) 572
Eco31I GGTCTC 4 cut(s) 277, 488, 1093, 1434
Eco57I CTGAAG 2 cut(s) 1164, 1185
Eco81I CCTNAGG 2 cut(s) 831, 1314
Eco88I CYCGRG 1 cut(s) 998
EcoRII CCWGG 7 cut(s) 605, 610, 667, 722, 727, 784, 1208
EcoT14I CCWWGG 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 572
ErhI CCWWGG 1 cut(s) 898
FaeI CATG 7 cut(s) 190, 214, 251, 566, 589, 902, 968
FaqI GGGAC 3 cut(s) 34, 378, 1386
FatI CATG 7 cut(s) 186, 210, 247, 562, 585, 898, 964
Fnu4HI GCNGC 4 cut(s) 540, 1152, 1233, 1278
FokI GGATG 6 cut(s) 67, 127, 848, 915, 1133, 1397
FriOI GRGCYC 1 cut(s) 572
Fsp4HI GCNGC 4 cut(s) 540, 1152, 1233, 1278
FspBI CTAG 4 cut(s) 426, 483, 1349, 1376
GlaI GCGC 1 cut(s) 1150
GluI GCNGC 4 cut(s) 540, 1152, 1233, 1278
GsuI CTGGAG 1 cut(s) 1231
HaeII RGCGCY 1 cut(s) 1152
HapII CCGG 3 cut(s) 86, 101, 227
HgaI GACGC 1 cut(s) 1238
HhaI GCGC 1 cut(s) 1151
Hin1II CATG 7 cut(s) 190, 214, 251, 566, 589, 902, 968
Hin6I GCGC 1 cut(s) 1149
HinP1I GCGC 1 cut(s) 1149
HincII GTYRAC 3 cut(s) 685, 700, 802
HindII GTYRAC 3 cut(s) 685, 700, 802
HinfI GANTC 5 cut(s) 345, 894, 971, 1160, 1178
HpaII CCGG 3 cut(s) 86, 101, 227
HphI GGTGA 7 cut(s) 102, 174, 216, 241, 683, 800, 1039
Hpy166II GTNNAC 7 cut(s) 241, 685, 700, 802, 817, 1030, 1325
Hpy188III TCNNGA 3 cut(s) 998, 1349, 1450
Hpy8I GTNNAC 7 cut(s) 241, 685, 700, 802, 817, 1030, 1325
Hpy99I CGWCG 2 cut(s) 521, 1232
HpyCH4III ACNGT 8 cut(s) 65, 135, 160, 416, 473, 521, 697, 814
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 5 cut(s) 80, 98, 256, 1235, 1280
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 95, 426, 483, 548
HpyF3I CTNAG 8 cut(s) 501, 552, 831, 845, 861, 1130, 1314, 1380
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 7 cut(s) 190, 214, 251, 566, 589, 902, 968
HspAI GCGC 1 cut(s) 1149
Kzo9I GATC 8 cut(s) 14, 202, 207, 617, 673, 734, 790, 989
LmnI GCTCC 2 cut(s) 832, 1084
Lsp1109I GCAGC 3 cut(s) 1138, 1219, 1264
LweI GCATC 3 cut(s) 14, 89, 243
MaeI CTAG 4 cut(s) 426, 483, 1349, 1376
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 54, 160, 982
MalI GATC 8 cut(s) 16, 204, 209, 619, 675, 736, 792, 991
MboI GATC 8 cut(s) 14, 202, 207, 617, 673, 734, 790, 989
MboII GAAGA 4 cut(s) 325, 503, 988, 1178
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 572
MluCI AATT 8 cut(s) 176, 293, 524, 663, 780, 852, 944, 1268
MlyI GAGTC 2 cut(s) 339, 888
MmeI TCCRAC 3 cut(s) 390, 447, 964
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 4 cut(s) 527, 678, 795, 1115
MspA1I CMGCKG 2 cut(s) 539, 1091
MspI CCGG 3 cut(s) 86, 101, 227
MspR9I CCNGG 8 cut(s) 86, 607, 612, 669, 724, 729, 786, 1210
MvaI CCWGG 7 cut(s) 607, 612, 669, 724, 729, 786, 1210
MvnI CGCG 1 cut(s) 1203
MwoI GCNNNNNNNGC 5 cut(s) 86, 95, 426, 483, 548
NciI CCSGG 1 cut(s) 86
NcoI CCATGG 1 cut(s) 898
NdeII GATC 8 cut(s) 14, 202, 207, 617, 673, 734, 790, 989
NlaIII CATG 7 cut(s) 190, 214, 251, 566, 589, 902, 968
NlaIV GGNNCC 5 cut(s) 105, 591, 708, 1086, 1245
NmuCI GTSAC 3 cut(s) 54, 160, 982
NspI RCATGY 1 cut(s) 968
PcsI WCGNNNNNNNCGW 1 cut(s) 1406
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 3 cut(s) 971, 1160, 1178
Pfl23II CGTACG 1 cut(s) 1182
PflFI GACNNNGTC 1 cut(s) 695
PinAI ACCGGT 2 cut(s) 100, 226
PkrI GCNGC 4 cut(s) 541, 1153, 1234, 1279
Ple19I CGATCG 2 cut(s) 620, 737
PleI GAGTC 2 cut(s) 339, 888
PpsI GAGTC 2 cut(s) 339, 888
Psp6I CCWGG 7 cut(s) 605, 610, 667, 722, 727, 784, 1208
PspGI CCWGG 7 cut(s) 605, 610, 667, 722, 727, 784, 1208
PspLI CGTACG 1 cut(s) 1182
PspN4I GGNNCC 5 cut(s) 105, 591, 708, 1086, 1245
PspPI GGNCC 2 cut(s) 637, 754
PstI CTGCAG 1 cut(s) 1237
PsuI RGATCY 1 cut(s) 14
PsyI GACNNNGTC 1 cut(s) 695
PvuI CGATCG 2 cut(s) 620, 737
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 2 cut(s) 1184, 1324
RsaNI GTAC 2 cut(s) 1183, 1323
SaqAI TTAA 4 cut(s) 527, 678, 795, 1115
SatI GCNGC 4 cut(s) 540, 1152, 1233, 1278
Sau3AI GATC 8 cut(s) 14, 202, 207, 617, 673, 734, 790, 989
Sau96I GGNCC 2 cut(s) 637, 754
SchI GAGTC 2 cut(s) 339, 888
ScrFI CCNGG 8 cut(s) 86, 607, 612, 669, 724, 729, 786, 1210
SduI GDGCHC 1 cut(s) 572
SfaNI GCATC 3 cut(s) 14, 89, 243
SfcI CTRYAG 1 cut(s) 1233
SgrAI CRCCGGYG 1 cut(s) 226
Sse9I AATT 8 cut(s) 176, 293, 524, 663, 780, 852, 944, 1268
SsiI CCGC 1 cut(s) 539
SspI AATATT 1 cut(s) 1113
SspMI CTAG 4 cut(s) 426, 483, 1349, 1376
StyD4I CCNGG 8 cut(s) 84, 605, 610, 667, 722, 727, 784, 1208
StyI CCWWGG 1 cut(s) 898
TaaI ACNGT 8 cut(s) 65, 135, 160, 416, 473, 521, 697, 814
TaiI ACGT 1 cut(s) 35
TaqI TCGA 4 cut(s) 195, 948, 974, 1409
TasI AATT 8 cut(s) 176, 293, 524, 663, 780, 852, 944, 1268
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 3 cut(s) 971, 1160, 1178
Tru1I TTAA 4 cut(s) 527, 678, 795, 1115
Tru9I TTAA 4 cut(s) 527, 678, 795, 1115
TscAI CASTG 6 cut(s) 63, 165, 421, 478, 505, 1162
TseFI GTSAC 3 cut(s) 54, 160, 982
TseI GCWGC 3 cut(s) 1151, 1232, 1277
Tsp45I GTSAC 3 cut(s) 54, 160, 982
TspDTI ATGAA 8 cut(s) 306, 321, 410, 467, 852, 1058, 1137, 1401
TspRI CASTG 6 cut(s) 63, 165, 421, 478, 505, 1162
Tth111I GACNNNGTC 1 cut(s) 695
XapI RAATTY 3 cut(s) 663, 780, 1268
XbaI TCTAGA 1 cut(s) 1348
XceI RCATGY 1 cut(s) 968
XcmI CCANNNNNNNNNTGG 2 cut(s) 631, 748
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 4 cut(s) 426, 483, 1349, 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.