Rh4AG032100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001250
Physical Location & Seq
Reverse (-)
6927693 .. 6929846
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG032100.1

Sequence Viewer

Length: 1272 bp
ATGGCATCACCAAGATCCTCCTTTTTCTCAACGTCATCCCCGTCACTGAACCGTAGCACTTCACTTGCATCCCCGGCTACAACTGCACCGGTTCCCTCACCACCCATTTTTGAGACTGGACTGTCGCCATCCACATATAAGCCGGCAGTGACACCAGAACCCCAATTTTCACCCATGCCGTTCGATTACGATCAGATCATGGACGACTTTTCACCGGTGAAAATAGTGGACTACCATGAGATGCAAAGAGGCCAAGTGGTCTCTCCATCTCTTTCAAATGAATTAGTTCTCAATGAACCGAAGACACAGCCACAAGAAAATATACAGGAACGACTCGGACAAGTTGTCAAGGGTCTTGTTCAAAAAGAGGTAGATAAGTTGATGAAAAAAGCAAAGTCGGACAGTGGCAGAACTAGCGTGGAAGAGACCACTGAGTTAGCGAAGGACGACGGTAAATTAAAGATAACAGCGGCAGAGAAGGCTCAGGCTGACCATGAGAGCCCTAAAATAACTGACATGGAACCGACCTTCACTACCAGGCCAGGCCGATCGGCCACCAAGCAAGTGGCCCATATTTCCAATGTGTCCTTTCAAATTCCTGGTGATCTTAAAGTTGACAATGACACAGTAAGCGAGTTTGCTCCTAAGGATGAAGTCCTCAGAGAAATTACTGCTCAGAGTAGGAAAAAGTATAGTTTCTCTGACAGACTCCATGGATGGAAAATGGAGTGTAGGATAAGACTGAATGGTATCCAACAATTCGATAAGTATTTTACGCATGTAGAATCGAAGATTGTGACAAGATCAGTTCCCGAGGTTGTGAGGTTTATCCTTTATGAGGTGAACCCTAAACAAATGAAGGAAGCCAATAGCAAACGCAAAGGGATAGATATAGGGGAGCCAGCTGATCAGAGACCTAAAAAAATATTAAAGGATAAATACCTAAACAAGTCATCTGAAGGCGCTGCCAGTGATTCTGAAGAAAAGAGGGAATCGTACGATGAGTTGTTTTTTCGCGTAGCCTGGAGTAATCTAATAAACGACGCTGCAGAGAAGGTGCCGTTATTTAGTGAATGGGAAAAATTCAAGGCTGCAAAAAAGAAGGCCAGAACTAAACCATGCGAACCTGAGGTTGGTACACCCACCTTGGATATTGAAGCTCTAGAGACAGCAAACGCCTTGATGGGACTAGCTGAGGATGAAAGTTTGGCTACGAGAAAGTCGAAGGCTTGTAATGATAGGTCTGGTTTTGGTGTTTTTGTCAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

423

Amino Acids

47.35

Weight (kDa)

7.63

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 121
AccB1I GGYRCC 1 cut(s) 1057
AccII CGCG 1 cut(s) 1017
AciI CCGC 1 cut(s) 470
AclWI GGATC 1 cut(s) 9
AcoI YGGCCR 1 cut(s) 552
AcsI RAATTY 2 cut(s) 594, 1082
AcuI CTGAAG 2 cut(s) 978, 999
AfaI GTAC 2 cut(s) 998, 1138
AfiI CCNNNNNNNGG 2 cut(s) 838, 1133
AgeI ACCGGT 2 cut(s) 88, 214
AgsI TTSAA 5 cut(s) 276, 362, 593, 1087, 1157
AhdI GACNNNNNGTC 1 cut(s) 344
AjnI CCWGG 4 cut(s) 536, 541, 598, 1022
AjuI GAANNNNNNNTTGG 2 cut(s) 1116, 1148
AluBI AGCT 3 cut(s) 905, 1160, 1193
AluI AGCT 3 cut(s) 905, 1160, 1193
Alw26I GTCTC 5 cut(s) 107, 265, 419, 907, 1160
AlwI GGATC 1 cut(s) 9
Ama87I CYCGRG 1 cut(s) 812
AoxI GGCC 6 cut(s) 250, 539, 544, 552, 567, 1104
ApeKI GCWGC 3 cut(s) 965, 1046, 1091
ApoI RAATTY 2 cut(s) 594, 1082
AsiGI ACCGGT 2 cut(s) 88, 214
Asp700I GAANNNNTTC 1 cut(s) 285
AspLEI GCGC 1 cut(s) 965
AspS9I GGNCC 1 cut(s) 568
AsuC2I CCSGG 1 cut(s) 74
AsuHPI GGTGA 6 cut(s) 90, 162, 204, 229, 614, 853
AvaI CYCGRG 1 cut(s) 812
AxyI CCTNAGG 2 cut(s) 645, 1128
BanI GGYRCC 1 cut(s) 1057
BanII GRGCYC 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 308
BbvCI CCTCAGC 1 cut(s) 1194
BbvI GCAGC 3 cut(s) 952, 1033, 1078
BccI CCATC 4 cut(s) 136, 274, 711, 1177
BceAI ACGGC 2 cut(s) 163, 1045
BciT130I CCWGG 4 cut(s) 538, 543, 600, 1024
BciVI GTATCC 1 cut(s) 761
BclI TGATCA 1 cut(s) 907
BcnI CCSGG 1 cut(s) 74
BcoDI GTCTC 5 cut(s) 107, 265, 419, 907, 1160
BfaI CTAG 3 cut(s) 414, 1163, 1190
BfmI CTRYAG 1 cut(s) 1047
BfoI RGCGCY 1 cut(s) 966
BfuI GTATCC 1 cut(s) 761
BisI GCNGC 4 cut(s) 471, 966, 1047, 1092
BlsI GCNGC 4 cut(s) 472, 967, 1048, 1093
Bme1390I CCNGG 5 cut(s) 74, 538, 543, 600, 1024
BmeRI GACNNNNNGTC 1 cut(s) 344
BmeT110I CYCGRG 1 cut(s) 812
BmgT120I GGNCC 1 cut(s) 568
BmiI GGNNCC 4 cut(s) 93, 522, 900, 1059
BmrFI CCNGG 5 cut(s) 74, 538, 543, 600, 1024
BmsI GCATC 3 cut(s) 14, 77, 231
BpiI GAAGAC 1 cut(s) 308
BpmI CTGGAG 1 cut(s) 1045
Bpu10I CCTNAGC 2 cut(s) 483, 1194
BpuMI CCSGG 1 cut(s) 74
BsaBI GATNNNNATC 1 cut(s) 189
BsaI GGTCTC 3 cut(s) 265, 419, 907
BsaJI CCNNGG 4 cut(s) 72, 712, 813, 1146
BsaWI WCCGGW 2 cut(s) 88, 214
Bsc4I CCNNNNNNNGG 2 cut(s) 838, 1133
Bse118I RCCGGY 3 cut(s) 88, 142, 214
Bse1I ACTGG 2 cut(s) 121, 969
Bse21I CCTNAGG 2 cut(s) 645, 1128
Bse8I GATNNNNATC 1 cut(s) 189
BseBI CCWGG 4 cut(s) 538, 543, 600, 1024
BseDI CCNNGG 4 cut(s) 72, 712, 813, 1146
BseGI GGATG 6 cut(s) 35, 68, 128, 655, 722, 1204
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 838, 1133
BseMII CTCAG 6 cut(s) 423, 497, 673, 689, 1119, 1185
BseNI ACTGG 2 cut(s) 121, 969
BseXI GCAGC 3 cut(s) 952, 1033, 1078
BsgI GTGCAG 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 1017
Bsh1285I CGRYCG 1 cut(s) 551
BshFI GGCC 6 cut(s) 252, 541, 546, 554, 569, 1106
BshNI GGYRCC 1 cut(s) 1057
BshTI ACCGGT 2 cut(s) 88, 214
BsiEI CGRYCG 1 cut(s) 551
BsiHKCI CYCGRG 1 cut(s) 812
BsiSI CCGG 4 cut(s) 74, 89, 143, 215
BsiWI CGTACG 1 cut(s) 996
BslFI GGGAC 1 cut(s) 1200
BslI CCNNNNNNNGG 2 cut(s) 838, 1133
BsmAI GTCTC 5 cut(s) 107, 265, 419, 907, 1160
BsmFI GGGAC 1 cut(s) 1200
BsnI GGCC 6 cut(s) 252, 541, 546, 554, 569, 1106
Bso31I GGTCTC 3 cut(s) 265, 419, 907
BsoBI CYCGRG 1 cut(s) 812
Bsp1286I GDGCHC 1 cut(s) 503
Bsp143I GATC 7 cut(s) 14, 190, 195, 548, 604, 803, 907
Bsp19I CCATGG 1 cut(s) 712
BspACI CCGC 1 cut(s) 470
BspANI GGCC 6 cut(s) 252, 541, 546, 554, 569, 1106
BspCNI CTCAG 6 cut(s) 424, 496, 672, 688, 1120, 1186
BspFNI CGCG 1 cut(s) 1017
BspLI GGNNCC 4 cut(s) 93, 522, 900, 1059
BspMAI CTGCAG 1 cut(s) 1051
BspPI GGATC 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 1057
BspTNI GGTCTC 3 cut(s) 265, 419, 907
BsrFI RCCGGY 3 cut(s) 88, 142, 214
BsrI ACTGG 2 cut(s) 121, 969
BssAI RCCGGY 3 cut(s) 88, 142, 214
BssECI CCNNGG 4 cut(s) 72, 712, 813, 1146
BssMI GATC 7 cut(s) 14, 190, 195, 548, 604, 803, 907
BssT1I CCWWGG 2 cut(s) 712, 1146
Bst2UI CCWGG 4 cut(s) 538, 543, 600, 1024
Bst4CI ACNGT 5 cut(s) 53, 123, 404, 452, 628
Bst6I CTCTTC 1 cut(s) 417
BstC8I GCNNGC 2 cut(s) 144, 903
BstDEI CTNAG 7 cut(s) 432, 483, 645, 659, 675, 1128, 1194
BstDSI CCRYGG 1 cut(s) 712
BstENI CCTNNNNNAGG 1 cut(s) 836
BstF5I GGATG 6 cut(s) 35, 68, 128, 655, 722, 1204
BstFNI CGCG 1 cut(s) 1017
BstH2I RGCGCY 1 cut(s) 966
BstHHI GCGC 1 cut(s) 965
BstKTI GATC 7 cut(s) 17, 193, 198, 551, 607, 806, 910
BstMAI GTCTC 5 cut(s) 107, 265, 419, 907, 1160
BstMBI GATC 7 cut(s) 14, 190, 195, 548, 604, 803, 907
BstMCI CGRYCG 1 cut(s) 551
BstMWI GCNNNNNNNGC 4 cut(s) 74, 83, 414, 479
BstNI CCWGG 4 cut(s) 538, 543, 600, 1024
BstNSI RCATGY 1 cut(s) 782
BstSCI CCNGG 5 cut(s) 72, 536, 541, 598, 1022
BstSFI CTRYAG 1 cut(s) 1047
BstUI CGCG 1 cut(s) 1017
BstV1I GCAGC 3 cut(s) 952, 1033, 1078
BstV2I GAAGAC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 14
BstXI CCANNNNNNTGG 1 cut(s) 565
BstYI RGATCY 1 cut(s) 14
Bsu36I CCTNAGG 2 cut(s) 645, 1128
BsuI GTATCC 1 cut(s) 761
BsuRI GGCC 6 cut(s) 252, 541, 546, 554, 569, 1106
BtgI CCRYGG 1 cut(s) 712
BtsCI GGATG 6 cut(s) 35, 68, 128, 655, 722, 1204
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 5 cut(s) 44, 153, 409, 429, 976
Cac8I GCNNGC 2 cut(s) 144, 903
CfoI GCGC 1 cut(s) 965
Cfr10I RCCGGY 3 cut(s) 88, 142, 214
Cfr13I GGNCC 1 cut(s) 568
CseI GACGC 1 cut(s) 1052
Csp6I GTAC 2 cut(s) 997, 1137
CspAI ACCGGT 2 cut(s) 88, 214
CviAII CATG 8 cut(s) 175, 199, 236, 494, 517, 713, 779, 1119
CviQI GTAC 2 cut(s) 997, 1137
DdeI CTNAG 7 cut(s) 432, 483, 645, 659, 675, 1128, 1194
DpnI GATC 7 cut(s) 16, 192, 197, 550, 606, 805, 909
DpnII GATC 7 cut(s) 14, 190, 195, 548, 604, 803, 907
DrdI GACNNNNNNGTC 1 cut(s) 121
DriI GACNNNNNGTC 1 cut(s) 344
DseDI GACNNNNNNGTC 1 cut(s) 121
EaeI YGGCCR 1 cut(s) 552
Eam1104I CTCTTC 1 cut(s) 417
Eam1105I GACNNNNNGTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 417
Eco130I CCWWGG 2 cut(s) 712, 1146
Eco24I GRGCYC 1 cut(s) 503
Eco31I GGTCTC 3 cut(s) 265, 419, 907
Eco57I CTGAAG 2 cut(s) 978, 999
Eco81I CCTNAGG 2 cut(s) 645, 1128
Eco88I CYCGRG 1 cut(s) 812
EcoNI CCTNNNNNAGG 1 cut(s) 836
EcoRII CCWGG 4 cut(s) 536, 541, 598, 1022
EcoT14I CCWWGG 2 cut(s) 712, 1146
EcoT38I GRGCYC 1 cut(s) 503
ErhI CCWWGG 2 cut(s) 712, 1146
FaeI CATG 8 cut(s) 178, 202, 239, 497, 520, 716, 782, 1122
FaqI GGGAC 1 cut(s) 1200
FatI CATG 8 cut(s) 174, 198, 235, 493, 516, 712, 778, 1118
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 4 cut(s) 471, 966, 1047, 1092
FokI GGATG 6 cut(s) 22, 55, 115, 662, 729, 1211
FriOI GRGCYC 1 cut(s) 503
Fsp4HI GCNGC 4 cut(s) 471, 966, 1047, 1092
FspBI CTAG 3 cut(s) 414, 1163, 1190
GlaI GCGC 1 cut(s) 964
GluI GCNGC 4 cut(s) 471, 966, 1047, 1092
GsuI CTGGAG 1 cut(s) 1045
HaeII RGCGCY 1 cut(s) 966
HaeIII GGCC 6 cut(s) 252, 541, 546, 554, 569, 1106
HapII CCGG 4 cut(s) 74, 89, 143, 215
HgaI GACGC 1 cut(s) 1052
HhaI GCGC 1 cut(s) 965
Hin1II CATG 8 cut(s) 178, 202, 239, 497, 520, 716, 782, 1122
Hin6I GCGC 1 cut(s) 963
HinP1I GCGC 1 cut(s) 963
HincII GTYRAC 1 cut(s) 616
HindII GTYRAC 1 cut(s) 616
HinfI GANTC 5 cut(s) 333, 708, 785, 974, 992
HpaII CCGG 4 cut(s) 74, 89, 143, 215
HphI GGTGA 6 cut(s) 90, 162, 204, 229, 614, 853
Hpy166II GTNNAC 4 cut(s) 229, 616, 844, 1139
Hpy188I TCNGA 9 cut(s) 195, 338, 400, 662, 678, 703, 912, 958, 979
Hpy188III TCNNGA 3 cut(s) 812, 1163, 1264
Hpy8I GTNNAC 4 cut(s) 229, 616, 844, 1139
Hpy99I CGWCG 2 cut(s) 452, 1046
HpyAV CCTTC 8 cut(s) 436, 472, 538, 853, 953, 1048, 1096, 1219
HpyCH4III ACNGT 5 cut(s) 53, 123, 404, 452, 628
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 5 cut(s) 68, 86, 244, 1049, 1094
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 83, 414, 479
HpyF3I CTNAG 7 cut(s) 432, 483, 645, 659, 675, 1128, 1194
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 8 cut(s) 178, 202, 239, 497, 520, 716, 782, 1122
HspAI GCGC 1 cut(s) 963
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 7 cut(s) 14, 190, 195, 548, 604, 803, 907
LmnI GCTCC 2 cut(s) 646, 898
Lsp1109I GCAGC 3 cut(s) 952, 1033, 1078
LweI GCATC 3 cut(s) 14, 77, 231
MaeI CTAG 3 cut(s) 414, 1163, 1190
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 42, 148, 796
MalI GATC 7 cut(s) 16, 192, 197, 550, 606, 805, 909
MboI GATC 7 cut(s) 14, 190, 195, 548, 604, 803, 907
MboII GAAGA 4 cut(s) 313, 434, 802, 992
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 503
MluCI AATT 7 cut(s) 164, 281, 455, 594, 666, 758, 1082
MlyI GAGTC 2 cut(s) 327, 702
MmeI TCCRAC 2 cut(s) 378, 778
MroNI GCCGGC 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 3 cut(s) 458, 609, 929
MspA1I CMGCKG 2 cut(s) 470, 905
MspI CCGG 4 cut(s) 74, 89, 143, 215
MspR9I CCNGG 5 cut(s) 74, 538, 543, 600, 1024
MvaI CCWGG 4 cut(s) 538, 543, 600, 1024
MvnI CGCG 1 cut(s) 1017
MwoI GCNNNNNNNGC 4 cut(s) 74, 83, 414, 479
NaeI GCCGGC 1 cut(s) 144
NciI CCSGG 1 cut(s) 74
NcoI CCATGG 1 cut(s) 712
NdeII GATC 7 cut(s) 14, 190, 195, 548, 604, 803, 907
NgoMIV GCCGGC 1 cut(s) 142
NlaIII CATG 8 cut(s) 178, 202, 239, 497, 520, 716, 782, 1122
NlaIV GGNNCC 4 cut(s) 93, 522, 900, 1059
NmuCI GTSAC 3 cut(s) 42, 148, 796
NspI RCATGY 1 cut(s) 782
PcsI WCGNNNNNNNCGW 2 cut(s) 38, 1220
PdiI GCCGGC 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 285
PfeI GAWTC 3 cut(s) 785, 974, 992
Pfl23II CGTACG 1 cut(s) 996
PinAI ACCGGT 2 cut(s) 88, 214
PkrI GCNGC 4 cut(s) 472, 967, 1048, 1093
Ple19I CGATCG 1 cut(s) 551
PleI GAGTC 2 cut(s) 327, 702
PpsI GAGTC 2 cut(s) 327, 702
Psp6I CCWGG 4 cut(s) 536, 541, 598, 1022
PspGI CCWGG 4 cut(s) 536, 541, 598, 1022
PspLI CGTACG 1 cut(s) 996
PspN4I GGNNCC 4 cut(s) 93, 522, 900, 1059
PspPI GGNCC 1 cut(s) 568
PstI CTGCAG 1 cut(s) 1051
PsuI RGATCY 1 cut(s) 14
PvuI CGATCG 1 cut(s) 551
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 2 cut(s) 998, 1138
RsaNI GTAC 2 cut(s) 997, 1137
SaqAI TTAA 3 cut(s) 458, 609, 929
SatI GCNGC 4 cut(s) 471, 966, 1047, 1092
Sau3AI GATC 7 cut(s) 14, 190, 195, 548, 604, 803, 907
Sau96I GGNCC 1 cut(s) 568
SchI GAGTC 2 cut(s) 327, 702
ScrFI CCNGG 5 cut(s) 74, 538, 543, 600, 1024
SduI GDGCHC 1 cut(s) 503
SfaNI GCATC 3 cut(s) 14, 77, 231
SfcI CTRYAG 1 cut(s) 1047
SgrAI CRCCGGYG 1 cut(s) 214
Sse9I AATT 7 cut(s) 164, 281, 455, 594, 666, 758, 1082
SsiI CCGC 1 cut(s) 470
SspI AATATT 1 cut(s) 927
SspMI CTAG 3 cut(s) 414, 1163, 1190
StyD4I CCNGG 5 cut(s) 72, 536, 541, 598, 1022
StyI CCWWGG 2 cut(s) 712, 1146
TaaI ACNGT 5 cut(s) 53, 123, 404, 452, 628
TaiI ACGT 1 cut(s) 35
TaqI TCGA 4 cut(s) 183, 762, 788, 1223
TasI AATT 7 cut(s) 164, 281, 455, 594, 666, 758, 1082
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 3 cut(s) 785, 974, 992
Tru1I TTAA 3 cut(s) 458, 609, 929
Tru9I TTAA 3 cut(s) 458, 609, 929
TscAI CASTG 5 cut(s) 51, 153, 409, 436, 976
TseFI GTSAC 3 cut(s) 42, 148, 796
TseI GCWGC 3 cut(s) 965, 1046, 1091
Tsp45I GTSAC 3 cut(s) 42, 148, 796
TspDTI ATGAA 6 cut(s) 294, 309, 398, 666, 872, 1215
TspRI CASTG 5 cut(s) 51, 153, 409, 436, 976
XagI CCTNNNNNAGG 1 cut(s) 836
XapI RAATTY 2 cut(s) 594, 1082
XbaI TCTAGA 1 cut(s) 1162
XceI RCATGY 1 cut(s) 782
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmnI GAANNNNTTC 1 cut(s) 285
XspI CTAG 3 cut(s) 414, 1163, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.