Rh4CG035200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001250
Physical Location & Seq
Reverse (-)
7217130 .. 7219295
2166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG035200.1

Sequence Viewer

Length: 1284 bp
ATGGCATCACCAAGATCCTCCTTTTTCTCAACGTGGAGGTCGCCATCGTCCCCGTCACTGAACCGTAGCACTTCACTTGCATCCCCGTCTACAACTGCACCGGTTCCCTCACCACCCATTTTTGAGACTGGACTGTCGCCATCCACATATAAGCCGACAGTGACACCAGAACCCCAATTTTCACCCATGCCGTTCGATTACGATCAGATCATGGACGACTTTTCACCGGTGAAAATAGTGGACTACCATGAGATGCAAAGAGGCCAAGTGGTCTCTCCATCTCCTTCAAATGAATTAGTTCTCAATGAACCGAAGACACAGCCACAAGAAAATATACAGGAACGACTCGGACAAGTTGTCAAGGGACTTGTTCAAAAAGAGGTAGATAAGTTGATGAAACAAGCAAAGTCGGACAGTGGCAGAACTAGCGTGGAAGAGACCACTGAGTTAGCGAAGGACGACGGTAAATTAAAGATAACAGCGGCAGAGAAGGCTCAGGCTGACCATGAGAGCCCTAAAATAACTGACATGGAACCGACCTTCACTACCAGGCCAGGCCGATCGGCCACCAAGCAAGTGGCCCATATTTCCAATGTGTCCTTTCAAATTCCTGGTGATCTTAAAGTTGACAATGACACAGTAAACGAGTTTGCTCCTAAGGATGAAGTCCTCAGAGAAATTACTGCTCAGAGTAGGAAAAAGTATAGTTTCTCTGACAGACTCCATGGATGGAAAATGGAGTGTAGGATAAGACTGAATGGTATCCAACAATTCGATAAGTATTTTACGCATGTAGAATCGAAGATTGTGACAAGATCAGTTCCCGAGGTTGTGAGGTTTATCCTTTATGAGGTGAACCCTAAACAAATGAAGGAAGCCAATAGCAAACGCAAAGGAATAGATATAGGGGAGCCAGCTGATGAGAGACCTAAAAAAATATTAAAGGATGAATACCTAAGCAAGTCATCTGAAGGCGCTGCCAGTGATTCTGAAGAAAAGAGGGAATCGTACGATGAGTTGTTTTTTCGCGTAGCCTGGAGTAATCTGATAAACGACGCTGCAGAGAAGGTGCCGTTATTTAGTGAATGGGAAAAATTCAAGGCTACAAAAAAGAAGGCCAGAACTAAACTATGCGAACCTGAGGTTGGTACACCCAACTTGGATATTGAAGCTCTAGAGACAGCAAACGCCTTGATGGGACTAGCTGAGGATGAAAGTTTGGCTACGAGAAAGTCGAAGGCTTGTAATGATAGGTCTCGTTTTGGTGTTTTTGTCAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

427

Amino Acids

48.06

Weight (kDa)

6.6

Isoelectric Point (pI)

54.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 1069
AccI GTMKAC 1 cut(s) 89
AccII CGCG 1 cut(s) 1029
AciI CCGC 1 cut(s) 482
AclWI GGATC 1 cut(s) 9
AcoI YGGCCR 1 cut(s) 564
AcsI RAATTY 2 cut(s) 606, 1094
AcuI CTGAAG 2 cut(s) 990, 1011
AfaI GTAC 2 cut(s) 1010, 1150
AfiI CCNNNNNNNGG 2 cut(s) 850, 1145
AgeI ACCGGT 2 cut(s) 100, 226
AgsI TTSAA 5 cut(s) 288, 374, 605, 1099, 1169
AhdI GACNNNNNGTC 1 cut(s) 356
AjnI CCWGG 4 cut(s) 548, 553, 610, 1034
AjuI GAANNNNNNNTTGG 2 cut(s) 1128, 1160
AluBI AGCT 3 cut(s) 917, 1172, 1205
AluI AGCT 3 cut(s) 917, 1172, 1205
Alw26I GTCTC 6 cut(s) 119, 277, 431, 919, 1172, 1260
AlwI GGATC 1 cut(s) 9
Ama87I CYCGRG 1 cut(s) 824
AoxI GGCC 6 cut(s) 262, 551, 556, 564, 579, 1116
ApeKI GCWGC 2 cut(s) 977, 1058
ApoI RAATTY 2 cut(s) 606, 1094
AsiGI ACCGGT 2 cut(s) 100, 226
Asp700I GAANNNNTTC 1 cut(s) 297
AspLEI GCGC 1 cut(s) 977
AspS9I GGNCC 1 cut(s) 580
AsuHPI GGTGA 6 cut(s) 102, 174, 216, 241, 626, 865
AvaI CYCGRG 1 cut(s) 824
AxyI CCTNAGG 2 cut(s) 657, 1140
BanI GGYRCC 1 cut(s) 1069
BanII GRGCYC 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 320
BbvCI CCTCAGC 1 cut(s) 1206
BbvI GCAGC 2 cut(s) 964, 1045
BccI CCATC 5 cut(s) 52, 148, 286, 723, 1189
BceAI ACGGC 2 cut(s) 175, 1057
BciT130I CCWGG 4 cut(s) 550, 555, 612, 1036
BciVI GTATCC 1 cut(s) 773
BcoDI GTCTC 6 cut(s) 119, 277, 431, 919, 1172, 1260
BfaI CTAG 3 cut(s) 426, 1175, 1202
BfmI CTRYAG 1 cut(s) 1059
BfoI RGCGCY 1 cut(s) 978
BfuI GTATCC 1 cut(s) 773
BisI GCNGC 3 cut(s) 483, 978, 1059
BlsI GCNGC 3 cut(s) 484, 979, 1060
Bme1390I CCNGG 4 cut(s) 550, 555, 612, 1036
BmeRI GACNNNNNGTC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 824
BmgT120I GGNCC 1 cut(s) 580
BmiI GGNNCC 4 cut(s) 105, 534, 912, 1071
BmrFI CCNGG 4 cut(s) 550, 555, 612, 1036
BmsI GCATC 3 cut(s) 14, 89, 243
BpiI GAAGAC 1 cut(s) 320
BpmI CTGGAG 1 cut(s) 1057
Bpu10I CCTNAGC 3 cut(s) 495, 956, 1206
BsaBI GATNNNNATC 1 cut(s) 201
BsaI GGTCTC 4 cut(s) 277, 431, 919, 1260
BsaJI CCNNGG 2 cut(s) 724, 825
BsaWI WCCGGW 2 cut(s) 100, 226
Bsc4I CCNNNNNNNGG 2 cut(s) 850, 1145
Bse118I RCCGGY 2 cut(s) 100, 226
Bse1I ACTGG 2 cut(s) 133, 981
Bse21I CCTNAGG 2 cut(s) 657, 1140
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 4 cut(s) 550, 555, 612, 1036
BseDI CCNNGG 2 cut(s) 724, 825
BseGI GGATG 6 cut(s) 80, 140, 667, 734, 952, 1216
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 2 cut(s) 850, 1145
BseMII CTCAG 6 cut(s) 435, 509, 685, 701, 1131, 1197
BseNI ACTGG 2 cut(s) 133, 981
BseXI GCAGC 2 cut(s) 964, 1045
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 1029
Bsh1285I CGRYCG 1 cut(s) 563
BshFI GGCC 6 cut(s) 264, 553, 558, 566, 581, 1118
BshNI GGYRCC 1 cut(s) 1069
BshTI ACCGGT 2 cut(s) 100, 226
BsiEI CGRYCG 1 cut(s) 563
BsiHKCI CYCGRG 1 cut(s) 824
BsiSI CCGG 2 cut(s) 101, 227
BsiWI CGTACG 1 cut(s) 1008
BslFI GGGAC 3 cut(s) 34, 378, 1212
BslI CCNNNNNNNGG 2 cut(s) 850, 1145
BsmAI GTCTC 6 cut(s) 119, 277, 431, 919, 1172, 1260
BsmFI GGGAC 3 cut(s) 34, 378, 1212
BsnI GGCC 6 cut(s) 264, 553, 558, 566, 581, 1118
Bso31I GGTCTC 4 cut(s) 277, 431, 919, 1260
BsoBI CYCGRG 1 cut(s) 824
Bsp1286I GDGCHC 1 cut(s) 515
Bsp143I GATC 6 cut(s) 14, 202, 207, 560, 616, 815
Bsp19I CCATGG 1 cut(s) 724
BspACI CCGC 1 cut(s) 482
BspANI GGCC 6 cut(s) 264, 553, 558, 566, 581, 1118
BspCNI CTCAG 6 cut(s) 436, 508, 684, 700, 1132, 1198
BspFNI CGCG 1 cut(s) 1029
BspLI GGNNCC 4 cut(s) 105, 534, 912, 1071
BspMAI CTGCAG 1 cut(s) 1063
BspPI GGATC 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 1069
BspTNI GGTCTC 4 cut(s) 277, 431, 919, 1260
BsrFI RCCGGY 2 cut(s) 100, 226
BsrI ACTGG 2 cut(s) 133, 981
BssAI RCCGGY 2 cut(s) 100, 226
BssECI CCNNGG 2 cut(s) 724, 825
BssMI GATC 6 cut(s) 14, 202, 207, 560, 616, 815
BssT1I CCWWGG 1 cut(s) 724
Bst2UI CCWGG 4 cut(s) 550, 555, 612, 1036
Bst4CI ACNGT 6 cut(s) 65, 135, 160, 416, 464, 640
Bst6I CTCTTC 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 915
BstDEI CTNAG 8 cut(s) 444, 495, 657, 671, 687, 956, 1140, 1206
BstDSI CCRYGG 1 cut(s) 724
BstENI CCTNNNNNAGG 1 cut(s) 848
BstF5I GGATG 6 cut(s) 80, 140, 667, 734, 952, 1216
BstFNI CGCG 1 cut(s) 1029
BstH2I RGCGCY 1 cut(s) 978
BstHHI GCGC 1 cut(s) 977
BstKTI GATC 6 cut(s) 17, 205, 210, 563, 619, 818
BstMAI GTCTC 6 cut(s) 119, 277, 431, 919, 1172, 1260
BstMBI GATC 6 cut(s) 14, 202, 207, 560, 616, 815
BstMCI CGRYCG 1 cut(s) 563
BstMWI GCNNNNNNNGC 2 cut(s) 426, 491
BstNI CCWGG 4 cut(s) 550, 555, 612, 1036
BstNSI RCATGY 1 cut(s) 794
BstSCI CCNGG 4 cut(s) 548, 553, 610, 1034
BstSFI CTRYAG 1 cut(s) 1059
BstUI CGCG 1 cut(s) 1029
BstV1I GCAGC 2 cut(s) 964, 1045
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 14
BstXI CCANNNNNNTGG 1 cut(s) 577
BstYI RGATCY 1 cut(s) 14
Bsu36I CCTNAGG 2 cut(s) 657, 1140
BsuI GTATCC 1 cut(s) 773
BsuRI GGCC 6 cut(s) 264, 553, 558, 566, 581, 1118
BtgI CCRYGG 1 cut(s) 724
BtsCI GGATG 6 cut(s) 80, 140, 667, 734, 952, 1216
BtsIMutI CAGTG 5 cut(s) 56, 165, 421, 441, 988
Cac8I GCNNGC 1 cut(s) 915
CfoI GCGC 1 cut(s) 977
Cfr10I RCCGGY 2 cut(s) 100, 226
Cfr13I GGNCC 1 cut(s) 580
CseI GACGC 1 cut(s) 1064
Csp6I GTAC 2 cut(s) 1009, 1149
CspAI ACCGGT 2 cut(s) 100, 226
CviAII CATG 7 cut(s) 187, 211, 248, 506, 529, 725, 791
CviQI GTAC 2 cut(s) 1009, 1149
DdeI CTNAG 8 cut(s) 444, 495, 657, 671, 687, 956, 1140, 1206
DpnI GATC 6 cut(s) 16, 204, 209, 562, 618, 817
DpnII GATC 6 cut(s) 14, 202, 207, 560, 616, 815
DrdI GACNNNNNNGTC 1 cut(s) 133
DriI GACNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 1 cut(s) 564
Eam1104I CTCTTC 1 cut(s) 429
Eam1105I GACNNNNNGTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 724
Eco24I GRGCYC 1 cut(s) 515
Eco31I GGTCTC 4 cut(s) 277, 431, 919, 1260
Eco57I CTGAAG 2 cut(s) 990, 1011
Eco81I CCTNAGG 2 cut(s) 657, 1140
Eco88I CYCGRG 1 cut(s) 824
EcoNI CCTNNNNNAGG 1 cut(s) 848
EcoRII CCWGG 4 cut(s) 548, 553, 610, 1034
EcoT14I CCWWGG 1 cut(s) 724
EcoT38I GRGCYC 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 724
FaeI CATG 7 cut(s) 190, 214, 251, 509, 532, 728, 794
FaqI GGGAC 3 cut(s) 34, 378, 1212
FatI CATG 7 cut(s) 186, 210, 247, 505, 528, 724, 790
FblI GTMKAC 1 cut(s) 89
Fnu4HI GCNGC 3 cut(s) 483, 978, 1059
FokI GGATG 6 cut(s) 67, 127, 674, 741, 959, 1223
FriOI GRGCYC 1 cut(s) 515
Fsp4HI GCNGC 3 cut(s) 483, 978, 1059
FspBI CTAG 3 cut(s) 426, 1175, 1202
GlaI GCGC 1 cut(s) 976
GluI GCNGC 3 cut(s) 483, 978, 1059
GsuI CTGGAG 1 cut(s) 1057
HaeII RGCGCY 1 cut(s) 978
HaeIII GGCC 6 cut(s) 264, 553, 558, 566, 581, 1118
HapII CCGG 2 cut(s) 101, 227
HgaI GACGC 1 cut(s) 1064
HhaI GCGC 1 cut(s) 977
Hin1II CATG 7 cut(s) 190, 214, 251, 509, 532, 728, 794
Hin6I GCGC 1 cut(s) 975
HinP1I GCGC 1 cut(s) 975
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HinfI GANTC 5 cut(s) 345, 720, 797, 986, 1004
HpaII CCGG 2 cut(s) 101, 227
HphI GGTGA 6 cut(s) 102, 174, 216, 241, 626, 865
Hpy166II GTNNAC 6 cut(s) 90, 241, 628, 643, 856, 1151
Hpy188I TCNGA 9 cut(s) 207, 350, 412, 674, 690, 715, 970, 991, 1047
Hpy188III TCNNGA 3 cut(s) 824, 1175, 1276
Hpy8I GTNNAC 6 cut(s) 90, 241, 628, 643, 856, 1151
Hpy99I CGWCG 2 cut(s) 464, 1058
HpyAV CCTTC 9 cut(s) 294, 448, 484, 550, 865, 965, 1060, 1108, 1231
HpyCH4III ACNGT 6 cut(s) 65, 135, 160, 416, 464, 640
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 4 cut(s) 80, 98, 256, 1061
HpyF10VI GCNNNNNNNGC 2 cut(s) 426, 491
HpyF3I CTNAG 8 cut(s) 444, 495, 657, 671, 687, 956, 1140, 1206
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 7 cut(s) 190, 214, 251, 509, 532, 728, 794
HspAI GCGC 1 cut(s) 975
Kzo9I GATC 6 cut(s) 14, 202, 207, 560, 616, 815
LmnI GCTCC 2 cut(s) 658, 910
Lsp1109I GCAGC 2 cut(s) 964, 1045
LweI GCATC 3 cut(s) 14, 89, 243
MaeI CTAG 3 cut(s) 426, 1175, 1202
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 54, 160, 808
MalI GATC 6 cut(s) 16, 204, 209, 562, 618, 817
MboI GATC 6 cut(s) 14, 202, 207, 560, 616, 815
MboII GAAGA 4 cut(s) 325, 446, 814, 1004
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 515
MluCI AATT 7 cut(s) 176, 293, 467, 606, 678, 770, 1094
MlyI GAGTC 2 cut(s) 339, 714
MmeI TCCRAC 2 cut(s) 390, 790
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 3 cut(s) 470, 621, 941
MspA1I CMGCKG 2 cut(s) 482, 917
MspI CCGG 2 cut(s) 101, 227
MspR9I CCNGG 4 cut(s) 550, 555, 612, 1036
MvaI CCWGG 4 cut(s) 550, 555, 612, 1036
MvnI CGCG 1 cut(s) 1029
MwoI GCNNNNNNNGC 2 cut(s) 426, 491
NcoI CCATGG 1 cut(s) 724
NdeII GATC 6 cut(s) 14, 202, 207, 560, 616, 815
NlaIII CATG 7 cut(s) 190, 214, 251, 509, 532, 728, 794
NlaIV GGNNCC 4 cut(s) 105, 534, 912, 1071
NmuCI GTSAC 3 cut(s) 54, 160, 808
NspI RCATGY 1 cut(s) 794
PcsI WCGNNNNNNNCGW 1 cut(s) 1232
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 3 cut(s) 797, 986, 1004
Pfl23II CGTACG 1 cut(s) 1008
PinAI ACCGGT 2 cut(s) 100, 226
PkrI GCNGC 3 cut(s) 484, 979, 1060
Ple19I CGATCG 1 cut(s) 563
PleI GAGTC 2 cut(s) 339, 714
PpsI GAGTC 2 cut(s) 339, 714
Psp6I CCWGG 4 cut(s) 548, 553, 610, 1034
PspGI CCWGG 4 cut(s) 548, 553, 610, 1034
PspLI CGTACG 1 cut(s) 1008
PspN4I GGNNCC 4 cut(s) 105, 534, 912, 1071
PspPI GGNCC 1 cut(s) 580
PstI CTGCAG 1 cut(s) 1063
PsuI RGATCY 1 cut(s) 14
PvuI CGATCG 1 cut(s) 563
PvuII CAGCTG 1 cut(s) 917
RsaI GTAC 2 cut(s) 1010, 1150
RsaNI GTAC 2 cut(s) 1009, 1149
SaqAI TTAA 3 cut(s) 470, 621, 941
SatI GCNGC 3 cut(s) 483, 978, 1059
Sau3AI GATC 6 cut(s) 14, 202, 207, 560, 616, 815
Sau96I GGNCC 1 cut(s) 580
SchI GAGTC 2 cut(s) 339, 714
ScrFI CCNGG 4 cut(s) 550, 555, 612, 1036
SduI GDGCHC 1 cut(s) 515
SfaNI GCATC 3 cut(s) 14, 89, 243
SfcI CTRYAG 1 cut(s) 1059
SgrAI CRCCGGYG 1 cut(s) 226
Sse9I AATT 7 cut(s) 176, 293, 467, 606, 678, 770, 1094
SsiI CCGC 1 cut(s) 482
SspI AATATT 1 cut(s) 939
SspMI CTAG 3 cut(s) 426, 1175, 1202
StyD4I CCNGG 4 cut(s) 548, 553, 610, 1034
StyI CCWWGG 1 cut(s) 724
TaaI ACNGT 6 cut(s) 65, 135, 160, 416, 464, 640
TaiI ACGT 1 cut(s) 35
TaqI TCGA 4 cut(s) 195, 774, 800, 1235
TasI AATT 7 cut(s) 176, 293, 467, 606, 678, 770, 1094
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 3 cut(s) 797, 986, 1004
Tru1I TTAA 3 cut(s) 470, 621, 941
Tru9I TTAA 3 cut(s) 470, 621, 941
TscAI CASTG 5 cut(s) 63, 165, 421, 448, 988
TseFI GTSAC 3 cut(s) 54, 160, 808
TseI GCWGC 2 cut(s) 977, 1058
Tsp45I GTSAC 3 cut(s) 54, 160, 808
TspDTI ATGAA 7 cut(s) 306, 321, 410, 678, 884, 963, 1227
TspRI CASTG 5 cut(s) 63, 165, 421, 448, 988
XagI CCTNNNNNAGG 1 cut(s) 848
XapI RAATTY 2 cut(s) 606, 1094
XbaI TCTAGA 1 cut(s) 1174
XceI RCATGY 1 cut(s) 794
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmiI GTMKAC 1 cut(s) 89
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 3 cut(s) 426, 1175, 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.