FvH4_5g20350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001250
Physical Location & Seq
Forward (+)
12016174 .. 12016920
747 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20350.t1

Sequence Viewer

Length: 387 bp
ATGATAGTCAATATTTTGGGAAACGCCGGATATGAAGGCGAAGAGGAAGATGATGCAGCTAAGAAGATTTCTATAGCAGCTAAATCAATCATCGAATCTATGATTTACCAGACGACTAAAGAGGAATCTACCATCACTGAAAGGATGGATGCATGCACTTCACATGAGACCAGTACCGGACGACGCTCGGCAAAGAAGAAGAAGACGAGCAGTAGGAGTACTAGAGTACTACCAAAGTTGCTGCTACAGCAAAGAAGAGAAAACCTGCACCAGACCCGAAGAAATGGATTCCGCTGTGTACGAGTGAATAGCTTGCCGGACGGTTGGGATCGGACCAGGCGGCCGGTTATGGATGAAATGAATTTGGCTTTCTTTAGAAAACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

129

Amino Acids

14.78

Weight (kDa)

10.0

Isoelectric Point (pI)

72.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 273
AciI CCGC 2 cut(s) 292, 340
AclI AACGTT 1 cut(s) 382
AclWI GGATC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 1 cut(s) 361
AfaI GTAC 4 cut(s) 175, 220, 228, 300
AjnI CCWGG 1 cut(s) 335
AluBI AGCT 3 cut(s) 59, 80, 312
AluI AGCT 3 cut(s) 59, 80, 312
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 1 cut(s) 336
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 3 cut(s) 56, 77, 241
ApoI RAATTY 1 cut(s) 361
AspS9I GGNCC 1 cut(s) 333
AvaII GGWCC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 3 cut(s) 68, 89, 228
BccI CCATC 2 cut(s) 139, 140
BciT130I CCWGG 1 cut(s) 337
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 2 cut(s) 72, 245
BfuAI ACCTGC 1 cut(s) 273
BisI GCNGC 4 cut(s) 57, 78, 242, 341
BlsI GCNGC 4 cut(s) 58, 79, 243, 342
BmcAI AGTACT 2 cut(s) 220, 228
Bme1390I CCNGG 1 cut(s) 337
Bme18I GGWCC 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 337
BmsI GCATC 2 cut(s) 43, 139
BpiI GAAGAC 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 161
BsaWI WCCGGW 1 cut(s) 176
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 171
BseBI CCWGG 1 cut(s) 337
BseGI GGATG 3 cut(s) 150, 154, 358
BseNI ACTGG 1 cut(s) 171
BseX3I CGGCCG 1 cut(s) 341
BseXI GCAGC 3 cut(s) 68, 89, 228
BsgI GTGCAG 1 cut(s) 251
Bsh1285I CGRYCG 1 cut(s) 344
BshFI GGCC 1 cut(s) 343
BsiEI CGRYCG 1 cut(s) 344
BsiSI CCGG 4 cut(s) 27, 177, 317, 344
BsmAI GTCTC 1 cut(s) 161
BsnI GGCC 1 cut(s) 343
Bso31I GGTCTC 1 cut(s) 161
Bsp143I GATC 1 cut(s) 328
BspACI CCGC 2 cut(s) 292, 340
BspANI GGCC 1 cut(s) 343
BspMI ACCTGC 1 cut(s) 273
BspPI GGATC 1 cut(s) 336
BspTNI GGTCTC 1 cut(s) 161
BsrFI RCCGGY 1 cut(s) 343
BsrI ACTGG 1 cut(s) 171
BssAI RCCGGY 1 cut(s) 343
BssMI GATC 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 323
Bst6I CTCTTC 2 cut(s) 36, 250
BstC8I GCNNGC 2 cut(s) 154, 314
BstDEI CTNAG 1 cut(s) 60
BstF5I GGATG 3 cut(s) 150, 154, 358
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 1 cut(s) 328
BstMCI CGRYCG 1 cut(s) 344
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNI CCWGG 1 cut(s) 337
BstNSI RCATGY 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 335
BstSFI CTRYAG 2 cut(s) 72, 245
BstV1I GCAGC 3 cut(s) 68, 89, 228
BstV2I GAAGAC 1 cut(s) 209
BstZI CGGCCG 1 cut(s) 341
BsuRI GGCC 1 cut(s) 343
BtsCI GGATG 3 cut(s) 150, 154, 358
BtsIMutI CAGTG 1 cut(s) 135
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 2 cut(s) 154, 314
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 333
CseI GACGC 1 cut(s) 192
Csp6I GTAC 4 cut(s) 174, 219, 227, 299
CviAII CATG 2 cut(s) 153, 164
CviJI RGCY 5 cut(s) 59, 80, 312, 343, 368
CviKI_1 RGCY 5 cut(s) 59, 80, 312, 343, 368
CviQI GTAC 4 cut(s) 174, 219, 227, 299
DdeI CTNAG 1 cut(s) 60
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 341
EagI CGGCCG 1 cut(s) 341
Eam1104I CTCTTC 2 cut(s) 36, 250
EarI CTCTTC 2 cut(s) 36, 250
EclXI CGGCCG 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 333
Eco52I CGGCCG 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 335
EcoT22I ATGCAT 1 cut(s) 154
FaeI CATG 2 cut(s) 156, 167
FaiI YATR 6 cut(s) 33, 74, 101, 154, 165, 350
FatI CATG 2 cut(s) 152, 163
Fnu4HI GCNGC 4 cut(s) 57, 78, 242, 341
FokI GGATG 3 cut(s) 157, 161, 365
Fsp4HI GCNGC 4 cut(s) 57, 78, 242, 341
FspBI CTAG 1 cut(s) 222
GluI GCNGC 4 cut(s) 57, 78, 242, 341
HaeIII GGCC 1 cut(s) 343
HapII CCGG 4 cut(s) 27, 177, 317, 344
HgaI GACGC 1 cut(s) 192
Hin1II CATG 2 cut(s) 156, 167
HinfI GANTC 3 cut(s) 95, 125, 288
HpaII CCGG 4 cut(s) 27, 177, 317, 344
Hpy166II GTNNAC 1 cut(s) 299
Hpy188I TCNGA 1 cut(s) 333
Hpy8I GTNNAC 1 cut(s) 299
Hpy99I CGWCG 1 cut(s) 186
HpyAV CCTTC 1 cut(s) 29
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 4 cut(s) 56, 152, 156, 268
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 2 cut(s) 156, 167
Kzo9I GATC 1 cut(s) 328
Lsp1109I GCAGC 3 cut(s) 68, 89, 228
LweI GCATC 2 cut(s) 43, 139
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 382
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 8 cut(s) 53, 59, 76, 208, 211, 214, 267, 291
MluCI AATT 1 cut(s) 361
MnlI CCTC 2 cut(s) 37, 115
Mph1103I ATGCAT 1 cut(s) 154
MseI TTAA 1 cut(s) 385
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 4 cut(s) 27, 177, 317, 344
MspR9I CCNGG 1 cut(s) 337
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 1 cut(s) 247
NdeII GATC 1 cut(s) 328
NlaIII CATG 2 cut(s) 156, 167
NmeAIII GCCGAG 1 cut(s) 167
NsiI ATGCAT 1 cut(s) 154
NspI RCATGY 1 cut(s) 156
PaeI GCATGC 1 cut(s) 156
PfeI GAWTC 3 cut(s) 95, 125, 288
PkrI GCNGC 4 cut(s) 58, 79, 243, 342
Psp1406I AACGTT 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspPI GGNCC 1 cut(s) 333
RsaI GTAC 4 cut(s) 175, 220, 228, 300
RsaNI GTAC 4 cut(s) 174, 219, 227, 299
SaqAI TTAA 1 cut(s) 385
SatI GCNGC 4 cut(s) 57, 78, 242, 341
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 1 cut(s) 333
ScaI AGTACT 2 cut(s) 220, 228
ScrFI CCNGG 1 cut(s) 337
SetI ASST 5 cut(s) 61, 82, 267, 314, 385
SfaNI GCATC 2 cut(s) 43, 139
SfcI CTRYAG 2 cut(s) 72, 245
SinI GGWCC 1 cut(s) 333
SphI GCATGC 1 cut(s) 156
Sse9I AATT 1 cut(s) 361
SsiI CCGC 2 cut(s) 292, 340
SspI AATATT 1 cut(s) 13
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 335
TaaI ACNGT 1 cut(s) 323
TaiI ACGT 1 cut(s) 385
TaqI TCGA 1 cut(s) 93
TasI AATT 1 cut(s) 361
TatI WGTACW 2 cut(s) 218, 226
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 3 cut(s) 95, 125, 288
Tru1I TTAA 1 cut(s) 385
Tru9I TTAA 1 cut(s) 385
TscAI CASTG 1 cut(s) 142
TseI GCWGC 3 cut(s) 56, 77, 241
TspDTI ATGAA 3 cut(s) 48, 369, 374
TspRI CASTG 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 333
XapI RAATTY 1 cut(s) 361
XceI RCATGY 1 cut(s) 156
XspI CTAG 1 cut(s) 222
ZrmI AGTACT 2 cut(s) 220, 228
Zsp2I ATGCAT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.