Rh4BG026200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001250
Physical Location & Seq
Reverse (-)
4558484 .. 4560651
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG026200.1

Sequence Viewer

Length: 1287 bp
ATGGCATCACCAAGATCCTCCTTTTTCTCAACGTGGAGGTCGCCATCATCCCCGTCACTGAACCGTAGCACTTCACTTGCATCCCCGGCTACAACTGCACCGGTTCCCTCACCACCCACTTTTGAGACTGGACTGTCGCCATCCACATATGAGCCGACAGTGACACCAGAACCCCAATTTTCACCCATGCCGTTCGATTACGATCAGATCATGGACGACTTTTCACCGGTGAAAATAGTGGACTACCATGAGATGCAAAGAGGCCAAGTGGTCTCTCCATCTCCTTCTTCAAATGAATTAGTTCTCAATGAACCGAAGACACGGCCACAAGAAAATATACAGGAACGACTCGGACAAGTTGTCAAGGGTCTTGTTCAAAAAGAGGTAGATAAGTTGATGAAACAAGCAAAGTCGGACAGTGGCAGAACTAGCGCGGAAGAGACCACTGAGTTAGCGAAGGACGACGGTAAATTAAAGATAACAGCCGCAGAGAAGGCTCAGGCTGACCATGAGAGCCCTAAAATAACTAACATGGAACCGACCTTCACTACCAGGCCAGGCCGATCGGCCACCAAGCAAGTGGCCCATATTTCCAATGTGTCCTTTCAAATTCATGATGATCTTAAAGTTGACAATGACACAGTAAACGAGTTTGCTCCTAAGGATGAAGTCCTCAGAGAAATTACTGCTCAGAGTAGGAAAAAGTATAGTTTCTCTGACAGACTCCATGGATGGAAAATGGAGTGTAGGATAAGACTGAATGGTATCCAACAATTCGATAAGTATTTTACGCATGTAGAATCGAAGATTGTGACAAGATCAGTTCCCGAGGTTGTGAGGTTTATCCTTTATGAGGTGAACCCTAAACAAATGAAGGAAGCCAATAGCAAACGCAAAGGGATAGATATAGGGGAGCTAGCTGATGAGAGACCTAAAAAAATATTAAAGGATGAATACCTAAACAAGTCATCTGAAGGCGCTGCCAGTGATTCTGAAGAAAAGAGGGAATCGTACGATGAGTTGTTTTTTCGCGTAGCCTGGAGTAATCTGATAAACGACGCTGCAGAGAAGGTGCCGTTATTTAGTGAATGGGAAAAATTCAAGGCTGCAAAAAAGAAGGCCAGAACTAAACCATGCGAACCTGAGGTTGGTACACCCAACTTGGATATTGAAGCTCTAGAGACAGCAAAGGCCTTGATGGGACTAGCTGAGGATGAAAGTTTGGCTACGAGAAAGTCGAAGGCTTGTAATGATAGGTCTGGTTTTGGTGTTTTTGTCAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

428

Amino Acids

48.13

Weight (kDa)

6.4

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 1072
AccII CGCG 2 cut(s) 434, 1032
AciI CCGC 2 cut(s) 434, 486
AclWI GGATC 1 cut(s) 9
AcoI YGGCCR 2 cut(s) 323, 567
AcsI RAATTY 2 cut(s) 609, 1097
AcuI CTGAAG 2 cut(s) 993, 1014
AfaI GTAC 2 cut(s) 1013, 1153
AfiI CCNNNNNNNGG 2 cut(s) 853, 1148
AgeI ACCGGT 2 cut(s) 100, 226
AgsI TTSAA 5 cut(s) 291, 377, 608, 1102, 1172
AhdI GACNNNNNGTC 1 cut(s) 359
AjnI CCWGG 3 cut(s) 551, 556, 1037
AjuI GAANNNNNNNTTGG 2 cut(s) 1131, 1163
AluBI AGCT 4 cut(s) 916, 920, 1175, 1208
AluI AGCT 4 cut(s) 916, 920, 1175, 1208
Alw26I GTCTC 5 cut(s) 119, 277, 434, 922, 1175
AlwI GGATC 1 cut(s) 9
Ama87I CYCGRG 1 cut(s) 827
AoxI GGCC 8 cut(s) 262, 323, 554, 559, 567, 582, 1119, 1191
ApeKI GCWGC 3 cut(s) 980, 1061, 1106
ApoI RAATTY 2 cut(s) 609, 1097
AsiGI ACCGGT 2 cut(s) 100, 226
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 2 cut(s) 434, 980
AspS9I GGNCC 1 cut(s) 583
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 5 cut(s) 102, 174, 216, 241, 868
AsuNHI GCTAGC 1 cut(s) 916
AvaI CYCGRG 1 cut(s) 827
AxyI CCTNAGG 2 cut(s) 660, 1143
BanI GGYRCC 1 cut(s) 1072
BanII GRGCYC 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 323
BbvCI CCTCAGC 1 cut(s) 1209
BbvI GCAGC 3 cut(s) 967, 1048, 1093
BccI CCATC 5 cut(s) 52, 148, 286, 726, 1192
BceAI ACGGC 3 cut(s) 175, 338, 1060
BciT130I CCWGG 3 cut(s) 553, 558, 1039
BciVI GTATCC 1 cut(s) 776
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 5 cut(s) 119, 277, 434, 922, 1175
BfaI CTAG 4 cut(s) 429, 917, 1178, 1205
BfmI CTRYAG 1 cut(s) 1062
BfoI RGCGCY 1 cut(s) 981
BfuI GTATCC 1 cut(s) 776
BisI GCNGC 4 cut(s) 486, 981, 1062, 1107
BlsI GCNGC 4 cut(s) 487, 982, 1063, 1108
Bme1390I CCNGG 4 cut(s) 86, 553, 558, 1039
BmeRI GACNNNNNGTC 1 cut(s) 359
BmeT110I CYCGRG 1 cut(s) 827
BmgT120I GGNCC 1 cut(s) 583
BmiI GGNNCC 3 cut(s) 105, 537, 1074
BmrFI CCNGG 4 cut(s) 86, 553, 558, 1039
BmsI GCATC 3 cut(s) 14, 89, 243
BmtI GCTAGC 1 cut(s) 920
BpiI GAAGAC 1 cut(s) 323
BpmI CTGGAG 1 cut(s) 1060
Bpu10I CCTNAGC 2 cut(s) 498, 1209
BpuMI CCSGG 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 201
BsaI GGTCTC 3 cut(s) 277, 434, 922
BsaJI CCNNGG 3 cut(s) 84, 727, 828
BsaWI WCCGGW 2 cut(s) 100, 226
Bsc4I CCNNNNNNNGG 2 cut(s) 853, 1148
Bse118I RCCGGY 2 cut(s) 100, 226
Bse1I ACTGG 2 cut(s) 133, 984
Bse21I CCTNAGG 2 cut(s) 660, 1143
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 3 cut(s) 553, 558, 1039
BseDI CCNNGG 3 cut(s) 84, 727, 828
BseGI GGATG 7 cut(s) 47, 80, 140, 670, 737, 955, 1219
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 2 cut(s) 853, 1148
BseMII CTCAG 6 cut(s) 438, 512, 688, 704, 1134, 1200
BseNI ACTGG 2 cut(s) 133, 984
BseXI GCAGC 3 cut(s) 967, 1048, 1093
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 2 cut(s) 434, 1032
Bsh1285I CGRYCG 1 cut(s) 566
BshFI GGCC 8 cut(s) 264, 325, 556, 561, 569, 584, 1121, 1193
BshNI GGYRCC 1 cut(s) 1072
BshTI ACCGGT 2 cut(s) 100, 226
BsiEI CGRYCG 1 cut(s) 566
BsiHKCI CYCGRG 1 cut(s) 827
BsiSI CCGG 3 cut(s) 86, 101, 227
BsiWI CGTACG 1 cut(s) 1011
BslFI GGGAC 1 cut(s) 1215
BslI CCNNNNNNNGG 2 cut(s) 853, 1148
BsmAI GTCTC 5 cut(s) 119, 277, 434, 922, 1175
BsmFI GGGAC 1 cut(s) 1215
BsnI GGCC 8 cut(s) 264, 325, 556, 561, 569, 584, 1121, 1193
Bso31I GGTCTC 3 cut(s) 277, 434, 922
BsoBI CYCGRG 1 cut(s) 827
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 6 cut(s) 14, 202, 207, 563, 619, 818
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 2 cut(s) 434, 486
BspANI GGCC 8 cut(s) 264, 325, 556, 561, 569, 584, 1121, 1193
BspCNI CTCAG 6 cut(s) 439, 511, 687, 703, 1135, 1201
BspFNI CGCG 2 cut(s) 434, 1032
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 3 cut(s) 105, 537, 1074
BspMAI CTGCAG 1 cut(s) 1066
BspOI GCTAGC 1 cut(s) 920
BspPI GGATC 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 1072
BspTNI GGTCTC 3 cut(s) 277, 434, 922
BsrFI RCCGGY 2 cut(s) 100, 226
BsrI ACTGG 2 cut(s) 133, 984
BssAI RCCGGY 2 cut(s) 100, 226
BssECI CCNNGG 3 cut(s) 84, 727, 828
BssMI GATC 6 cut(s) 14, 202, 207, 563, 619, 818
BssT1I CCWWGG 1 cut(s) 727
Bst2UI CCWGG 3 cut(s) 553, 558, 1039
Bst4CI ACNGT 6 cut(s) 65, 135, 160, 419, 467, 643
Bst6I CTCTTC 1 cut(s) 432
BstC8I GCNNGC 1 cut(s) 918
BstDEI CTNAG 7 cut(s) 447, 498, 660, 674, 690, 1143, 1209
BstDSI CCRYGG 1 cut(s) 727
BstENI CCTNNNNNAGG 1 cut(s) 851
BstF5I GGATG 7 cut(s) 47, 80, 140, 670, 737, 955, 1219
BstFNI CGCG 2 cut(s) 434, 1032
BstH2I RGCGCY 1 cut(s) 981
BstHHI GCGC 2 cut(s) 434, 980
BstKTI GATC 6 cut(s) 17, 205, 210, 566, 622, 821
BstMAI GTCTC 5 cut(s) 119, 277, 434, 922, 1175
BstMBI GATC 6 cut(s) 14, 202, 207, 563, 619, 818
BstMCI CGRYCG 1 cut(s) 566
BstMWI GCNNNNNNNGC 4 cut(s) 86, 95, 429, 494
BstNI CCWGG 3 cut(s) 553, 558, 1039
BstNSI RCATGY 1 cut(s) 797
BstSCI CCNGG 4 cut(s) 84, 551, 556, 1037
BstSFI CTRYAG 1 cut(s) 1062
BstUI CGCG 2 cut(s) 434, 1032
BstV1I GCAGC 3 cut(s) 967, 1048, 1093
BstV2I GAAGAC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 14
BstXI CCANNNNNNTGG 1 cut(s) 580
BstYI RGATCY 1 cut(s) 14
Bsu36I CCTNAGG 2 cut(s) 660, 1143
BsuI GTATCC 1 cut(s) 776
BsuRI GGCC 8 cut(s) 264, 325, 556, 561, 569, 584, 1121, 1193
BtgI CCRYGG 1 cut(s) 727
BtsCI GGATG 7 cut(s) 47, 80, 140, 670, 737, 955, 1219
BtsIMutI CAGTG 5 cut(s) 56, 165, 424, 444, 991
Cac8I GCNNGC 1 cut(s) 918
CciI TCATGA 1 cut(s) 613
CfoI GCGC 2 cut(s) 434, 980
Cfr10I RCCGGY 2 cut(s) 100, 226
Cfr13I GGNCC 1 cut(s) 583
CseI GACGC 1 cut(s) 1067
Csp6I GTAC 2 cut(s) 1012, 1152
CspAI ACCGGT 2 cut(s) 100, 226
CviAII CATG 9 cut(s) 187, 211, 248, 509, 532, 614, 728, 794, 1134
CviQI GTAC 2 cut(s) 1012, 1152
DdeI CTNAG 7 cut(s) 447, 498, 660, 674, 690, 1143, 1209
DpnI GATC 6 cut(s) 16, 204, 209, 565, 621, 820
DpnII GATC 6 cut(s) 14, 202, 207, 563, 619, 818
DrdI GACNNNNNNGTC 1 cut(s) 133
DriI GACNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 2 cut(s) 323, 567
Eam1104I CTCTTC 1 cut(s) 432
Eam1105I GACNNNNNGTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 432
Eco130I CCWWGG 1 cut(s) 727
Eco147I AGGCCT 1 cut(s) 1193
Eco24I GRGCYC 1 cut(s) 518
Eco31I GGTCTC 3 cut(s) 277, 434, 922
Eco57I CTGAAG 2 cut(s) 993, 1014
Eco81I CCTNAGG 2 cut(s) 660, 1143
Eco88I CYCGRG 1 cut(s) 827
EcoNI CCTNNNNNAGG 1 cut(s) 851
EcoRII CCWGG 3 cut(s) 551, 556, 1037
EcoT14I CCWWGG 1 cut(s) 727
EcoT38I GRGCYC 1 cut(s) 518
ErhI CCWWGG 1 cut(s) 727
FaeI CATG 9 cut(s) 190, 214, 251, 512, 535, 617, 731, 797, 1137
FaqI GGGAC 1 cut(s) 1215
FatI CATG 9 cut(s) 186, 210, 247, 508, 531, 613, 727, 793, 1133
FauNDI CATATG 1 cut(s) 148
Fnu4HI GCNGC 4 cut(s) 486, 981, 1062, 1107
FokI GGATG 7 cut(s) 34, 67, 127, 677, 744, 962, 1226
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 4 cut(s) 486, 981, 1062, 1107
FspBI CTAG 4 cut(s) 429, 917, 1178, 1205
GlaI GCGC 2 cut(s) 433, 979
GluI GCNGC 4 cut(s) 486, 981, 1062, 1107
GsuI CTGGAG 1 cut(s) 1060
HaeII RGCGCY 1 cut(s) 981
HaeIII GGCC 8 cut(s) 264, 325, 556, 561, 569, 584, 1121, 1193
HapII CCGG 3 cut(s) 86, 101, 227
HgaI GACGC 1 cut(s) 1067
HhaI GCGC 2 cut(s) 434, 980
Hin1II CATG 9 cut(s) 190, 214, 251, 512, 535, 617, 731, 797, 1137
Hin6I GCGC 2 cut(s) 432, 978
HinP1I GCGC 2 cut(s) 432, 978
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 5 cut(s) 348, 723, 800, 989, 1007
HpaII CCGG 3 cut(s) 86, 101, 227
HphI GGTGA 5 cut(s) 102, 174, 216, 241, 868
Hpy166II GTNNAC 5 cut(s) 241, 631, 646, 859, 1154
Hpy188I TCNGA 9 cut(s) 207, 353, 415, 677, 693, 718, 973, 994, 1050
Hpy188III TCNNGA 4 cut(s) 614, 827, 1178, 1279
Hpy8I GTNNAC 5 cut(s) 241, 631, 646, 859, 1154
Hpy99I CGWCG 2 cut(s) 467, 1061
HpyAV CCTTC 9 cut(s) 294, 451, 487, 553, 868, 968, 1063, 1111, 1234
HpyCH4III ACNGT 6 cut(s) 65, 135, 160, 419, 467, 643
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 5 cut(s) 80, 98, 256, 1064, 1109
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 95, 429, 494
HpyF3I CTNAG 7 cut(s) 447, 498, 660, 674, 690, 1143, 1209
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 9 cut(s) 190, 214, 251, 512, 535, 617, 731, 797, 1137
HspAI GCGC 2 cut(s) 432, 978
Kzo9I GATC 6 cut(s) 14, 202, 207, 563, 619, 818
LmnI GCTCC 2 cut(s) 661, 913
Lsp1109I GCAGC 3 cut(s) 967, 1048, 1093
LweI GCATC 3 cut(s) 14, 89, 243
MaeI CTAG 4 cut(s) 429, 917, 1178, 1205
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 54, 160, 811
MalI GATC 6 cut(s) 16, 204, 209, 565, 621, 820
MboI GATC 6 cut(s) 14, 202, 207, 563, 619, 818
MboII GAAGA 5 cut(s) 279, 328, 449, 817, 1007
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 7 cut(s) 176, 296, 470, 609, 681, 773, 1097
MlyI GAGTC 2 cut(s) 342, 717
MmeI TCCRAC 2 cut(s) 393, 793
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 3 cut(s) 473, 624, 944
MspI CCGG 3 cut(s) 86, 101, 227
MspR9I CCNGG 4 cut(s) 86, 553, 558, 1039
MvaI CCWGG 3 cut(s) 553, 558, 1039
MvnI CGCG 2 cut(s) 434, 1032
MwoI GCNNNNNNNGC 4 cut(s) 86, 95, 429, 494
NciI CCSGG 1 cut(s) 86
NcoI CCATGG 1 cut(s) 727
NdeI CATATG 1 cut(s) 148
NdeII GATC 6 cut(s) 14, 202, 207, 563, 619, 818
NheI GCTAGC 1 cut(s) 916
NlaIII CATG 9 cut(s) 190, 214, 251, 512, 535, 617, 731, 797, 1137
NlaIV GGNNCC 3 cut(s) 105, 537, 1074
NmuCI GTSAC 3 cut(s) 54, 160, 811
NspI RCATGY 1 cut(s) 797
PagI TCATGA 1 cut(s) 613
PceI AGGCCT 1 cut(s) 1193
PcsI WCGNNNNNNNCGW 1 cut(s) 1235
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 3 cut(s) 800, 989, 1007
Pfl23II CGTACG 1 cut(s) 1011
PinAI ACCGGT 2 cut(s) 100, 226
PkrI GCNGC 4 cut(s) 487, 982, 1063, 1108
Ple19I CGATCG 1 cut(s) 566
PleI GAGTC 2 cut(s) 342, 717
PpsI GAGTC 2 cut(s) 342, 717
Psp6I CCWGG 3 cut(s) 551, 556, 1037
PspGI CCWGG 3 cut(s) 551, 556, 1037
PspLI CGTACG 1 cut(s) 1011
PspN4I GGNNCC 3 cut(s) 105, 537, 1074
PspPI GGNCC 1 cut(s) 583
PstI CTGCAG 1 cut(s) 1066
PsuI RGATCY 1 cut(s) 14
PvuI CGATCG 1 cut(s) 566
RsaI GTAC 2 cut(s) 1013, 1153
RsaNI GTAC 2 cut(s) 1012, 1152
SaqAI TTAA 3 cut(s) 473, 624, 944
SatI GCNGC 4 cut(s) 486, 981, 1062, 1107
Sau3AI GATC 6 cut(s) 14, 202, 207, 563, 619, 818
Sau96I GGNCC 1 cut(s) 583
SchI GAGTC 2 cut(s) 342, 717
ScrFI CCNGG 4 cut(s) 86, 553, 558, 1039
SduI GDGCHC 1 cut(s) 518
SfaNI GCATC 3 cut(s) 14, 89, 243
SfcI CTRYAG 1 cut(s) 1062
SgrAI CRCCGGYG 1 cut(s) 226
Sse9I AATT 7 cut(s) 176, 296, 470, 609, 681, 773, 1097
SseBI AGGCCT 1 cut(s) 1193
SsiI CCGC 2 cut(s) 434, 486
SspI AATATT 1 cut(s) 942
SspMI CTAG 4 cut(s) 429, 917, 1178, 1205
StuI AGGCCT 1 cut(s) 1193
StyD4I CCNGG 4 cut(s) 84, 551, 556, 1037
StyI CCWWGG 1 cut(s) 727
TaaI ACNGT 6 cut(s) 65, 135, 160, 419, 467, 643
TaiI ACGT 1 cut(s) 35
TaqI TCGA 4 cut(s) 195, 777, 803, 1238
TasI AATT 7 cut(s) 176, 296, 470, 609, 681, 773, 1097
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 3 cut(s) 800, 989, 1007
Tru1I TTAA 3 cut(s) 473, 624, 944
Tru9I TTAA 3 cut(s) 473, 624, 944
TscAI CASTG 5 cut(s) 63, 165, 424, 451, 991
TseFI GTSAC 3 cut(s) 54, 160, 811
TseI GCWGC 3 cut(s) 980, 1061, 1106
Tsp45I GTSAC 3 cut(s) 54, 160, 811
TspDTI ATGAA 8 cut(s) 309, 324, 413, 602, 681, 887, 966, 1230
TspRI CASTG 5 cut(s) 63, 165, 424, 451, 991
XagI CCTNNNNNAGG 1 cut(s) 851
XapI RAATTY 2 cut(s) 609, 1097
XbaI TCTAGA 1 cut(s) 1177
XceI RCATGY 1 cut(s) 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 4 cut(s) 429, 917, 1178, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.