FvH4_4g27440
MADS Family

MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000389
Physical Location & Seq
Forward (+)
28436562 .. 28442707
6146 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27440.t1

Sequence Viewer

Length: 525 bp
ATGGTGAAGCCGGCGAGCAAGAAGATAAAGATCGAAAAGATTGACAACTTGCCGGCGAGGCAAGTGACGTATTCGAAGAGGAGAAGAGGGCTTCTGAAGAAAGCTAGAGAGCTTTCAGTTCTCTGTGATTGTGAGTTTTCTGTCATCATATTTTCTGCTACTGGCAAGCTCTGTGAGTCCTCCAGCTCCAGTACGAAGGATGTCATCACGAGGTATGAATCGCAGACGGGCGATGAGGGAAACCTGGACCAGTCATCTCTTGATCTCCAGCATGATTGCATCAAGTTGAGTAACGAAATTGCGGACAAGAGCCGTGTGCTAAGGCAGATGAATGGAGAGGATCTAGAAGGGCTGAACATAGATGAGTTGCAGAGATTGGAGACAAAGATTGATGGAAGTCTTAACCGTGTGCTTCAAACTAAGGAAGAAAATATTATCGGTGAAATCTTAGCACTTGAGGCAAAGGGAGCTGAGTTGGAACTAGCAAATAACCAATTGAGGCAGGAGGCAAGTGATAATACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000060 GO:0000900 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006417 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0009266 GO:0009628 GO:0009719 GO:0009725 GO:0009739 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009908 GO:0009909 GO:0009910 GO:0010033 GO:0010073 GO:0010074 GO:0010077 GO:0010219 GO:0010220 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0015031 GO:0015833 GO:0017038 GO:0017148 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0030371 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0033993 GO:0034248 GO:0034249 GO:0034504 GO:0034613 GO:0040034 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045182 GO:0045184 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046907 GO:0046982 GO:0046983 GO:0048367 GO:0048438 GO:0048506 GO:0048507 GO:0048509 GO:0048510 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051169 GO:0051170 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051641 GO:0051649 GO:0060255 GO:0061458 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0080090 GO:0080134 GO:0090079 GO:0090567 GO:0097159 GO:0098727 GO:0140110 GO:1901363 GO:1901700 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04022 ko04371 ko05418 map04013 map04022 map04371 map05418
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

175

Amino Acids

19.45

Weight (kDa)

5.25

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 13 - 58 6.9e-20 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 87 - 169 3.9e-15 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000389)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03630 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_5g35400 FvH4_5g35400 FvH4_5g35400 FvH4_5g35400 FvH4_5g35401 FvH4_5g35401
malus_domestica MD08G1196900.v1.1 MD08G1197000.v1.1 MD08G1197200.v1.1 MD08G1197300.v1.1 MD15G1384500.v1.1 MD15G1384600.v1.1
prunus_persica Prupe.1G531100_v2.0.a1 Prupe.1G531100_v2.0.a1 Prupe.1G531400_v2.0.a1 Prupe.1G531600_v2.0.a1 Prupe.1G531700_v2.0.a1
pyrus_communis pycom08g16910 pycom08g16940 pycom08g16960 pycom15g34480 pycom15g34490
rosa_chinensis RchiOBHm_Chr4g0434911 RchiOBHm_Chr7g0205051 RchiOBHm_Chr7g0205061 RchiOBHm_Chr7g0205071 RchiOBHm_Chr7g0236801 RchiOBHm_Chr7g0236811
rosa_laevigata RLG00000001060 RLG00000003404 RLG00000003405 RLG00000006636
rosa_multiflora Rmu_sc0008447.1_g000001 Rmu_sc0008851.1_g000011 Rmu_sc0008851.1_g000015
rosa_roxburghii Rroxscaffold_3G00224810 Rroxscaffold_3G00224820 Rroxscaffold_3G00252600 Rroxscaffold_5G00376120
rosa_rugosa Rorug04G0280500 Rorug04G0280600 Rorug04G0280700 Rorug04G0280800 Rorug04G0280900 Rorug04G0281000 Rorug04G0281100 Rorug07G0087100 Rorug07G0087200 Rorug07G0087200 Rorug07G0087300 Rorug07G0299300 Rorug07G0299400 Rorug07G0299500
rosa_samantha Rh4AG336000 Rh4BG344500 Rh4CG358900 Rh4DG338500 Rh7AG218200 Rh7AG218300 Rh7AG454500 Rh7AG454700 Rh7BG214900 Rh7BG224100 Rh7BG425700 Rh7BG425800 Rh7BG425900 Rh7CG231500 Rh7CG473200 Rh7CG473300 Rh7DG225600 Rh7DG225800 Rh7DG442500 Rh7DG442600
rosa_wichuraiana Rw4G029320 Rw7G018890 Rw7G018930 Rw7G037730 Rw7G037740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 302
AclWI GGATC 1 cut(s) 348
AcuI CTGAAG 1 cut(s) 116
AfaI GTAC 1 cut(s) 193
AgsI TTSAA 1 cut(s) 416
AjnI CCWGG 1 cut(s) 243
AluBI AGCT 5 cut(s) 104, 112, 169, 186, 470
AluI AGCT 5 cut(s) 104, 112, 169, 186, 470
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 1 cut(s) 348
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 2 cut(s) 16, 452
AsuII TTCGAA 1 cut(s) 74
AvaII GGWCC 1 cut(s) 247
BauI CACGAG 1 cut(s) 208
BccI CCATC 1 cut(s) 386
BceAI ACGGC 1 cut(s) 297
BciT130I CCWGG 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 3 cut(s) 105, 344, 482
BglI GCCNNNNNGGC 1 cut(s) 58
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 247
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 1 cut(s) 288
BpmI CTGGAG 3 cut(s) 166, 172, 251
Bpu10I CCTNAGC 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 29
Bse118I RCCGGY 2 cut(s) 10, 52
Bse1I ACTGG 3 cut(s) 166, 189, 250
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 1 cut(s) 245
BseGI GGATG 1 cut(s) 205
BseJI GATNNNNATC 1 cut(s) 29
BseMII CTCAG 1 cut(s) 462
BseNI ACTGG 3 cut(s) 166, 189, 250
BseRI GAGGAG 1 cut(s) 94
BsiSI CCGG 2 cut(s) 11, 53
BsmAI GTCTC 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 74
Bsp143I GATC 3 cut(s) 30, 262, 340
BspACI CCGC 1 cut(s) 302
BspCNI CTCAG 1 cut(s) 463
BspPI GGATC 1 cut(s) 348
BspT104I TTCGAA 1 cut(s) 74
BsrFI RCCGGY 2 cut(s) 10, 52
BsrI ACTGG 3 cut(s) 166, 189, 250
BssAI RCCGGY 2 cut(s) 10, 52
BssMI GATC 3 cut(s) 30, 262, 340
BssSI CACGAG 1 cut(s) 208
Bst2BI CACGAG 1 cut(s) 208
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 1 cut(s) 407
Bst6I CTCTTC 2 cut(s) 71, 79
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 12, 16, 54, 167
BstDEI CTNAG 4 cut(s) 320, 420, 448, 471
BstF5I GGATG 1 cut(s) 205
BstKTI GATC 3 cut(s) 33, 265, 343
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 3 cut(s) 30, 262, 340
BstMWI GCNNNNNNNGC 3 cut(s) 58, 458, 467
BstNI CCWGG 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 243
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 1 cut(s) 205
Cac8I GCNNGC 4 cut(s) 12, 16, 54, 167
Cfr10I RCCGGY 2 cut(s) 10, 52
Cfr13I GGNCC 1 cut(s) 247
Csp6I GTAC 1 cut(s) 192
CviAII CATG 1 cut(s) 272
CviJI RGCY 9 cut(s) 10, 91, 104, 112, 169, 186, 312, 352, 470
CviKI_1 RGCY 9 cut(s) 10, 91, 104, 112, 169, 186, 312, 352, 470
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 4 cut(s) 320, 420, 448, 471
DpnI GATC 3 cut(s) 32, 264, 342
DpnII GATC 3 cut(s) 30, 262, 340
Eam1104I CTCTTC 2 cut(s) 71, 79
EarI CTCTTC 2 cut(s) 71, 79
Eco47I GGWCC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 243
FaeI CATG 1 cut(s) 275
FaiI YATR 4 cut(s) 149, 216, 273, 359
FatI CATG 1 cut(s) 271
FokI GGATG 1 cut(s) 212
FspBI CTAG 3 cut(s) 105, 344, 482
GsuI CTGGAG 3 cut(s) 166, 172, 251
HapII CCGG 2 cut(s) 11, 53
Hin1II CATG 1 cut(s) 275
HinfI GANTC 2 cut(s) 176, 218
HpaII CCGG 2 cut(s) 11, 53
HphI GGTGA 2 cut(s) 16, 452
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 3 cut(s) 208, 260, 344
HpyAV CCTTC 2 cut(s) 190, 341
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4IV ACGT 1 cut(s) 68
HpyCH4V TGCA 2 cut(s) 279, 370
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 458, 467
HpyF3I CTNAG 4 cut(s) 320, 420, 448, 471
HpySE526I ACGT 1 cut(s) 68
Hsp92II CATG 1 cut(s) 275
KroI GCCGGC 2 cut(s) 10, 52
KroNI GCCGGC 2 cut(s) 12, 54
Kzo9I GATC 3 cut(s) 30, 262, 340
LmnI GCTCC 2 cut(s) 191, 467
LweI GCATC 1 cut(s) 288
MaeI CTAG 3 cut(s) 105, 344, 482
MaeII ACGT 1 cut(s) 68
MaeIII GTNAC 2 cut(s) 64, 290
MalI GATC 3 cut(s) 32, 264, 342
MboI GATC 3 cut(s) 30, 262, 340
MboII GAAGA 5 cut(s) 34, 88, 96, 109, 437
MfeI CAATTG 1 cut(s) 494
MflI RGATCY 1 cut(s) 340
MluCI AATT 2 cut(s) 297, 494
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 456
MroNI GCCGGC 2 cut(s) 10, 52
MseI TTAA 1 cut(s) 402
MspI CCGG 2 cut(s) 11, 53
MspR9I CCNGG 1 cut(s) 245
MunI CAATTG 1 cut(s) 494
MvaI CCWGG 1 cut(s) 245
MwoI GCNNNNNNNGC 3 cut(s) 58, 458, 467
NaeI GCCGGC 2 cut(s) 12, 54
NdeII GATC 3 cut(s) 30, 262, 340
NgoMIV GCCGGC 2 cut(s) 10, 52
NlaIII CATG 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 64
NspV TTCGAA 1 cut(s) 74
PdiI GCCGGC 2 cut(s) 12, 54
PfeI GAWTC 1 cut(s) 218
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 243
PspGI CCWGG 1 cut(s) 243
PspPI GGNCC 1 cut(s) 247
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
SaqAI TTAA 1 cut(s) 402
Sau3AI GATC 3 cut(s) 30, 262, 340
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 245
SetI ASST 9 cut(s) 71, 106, 114, 171, 188, 215, 246, 472, 524
SfaNI GCATC 1 cut(s) 288
SfuI TTCGAA 1 cut(s) 74
SinI GGWCC 1 cut(s) 247
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 2 cut(s) 297, 494
SsiI CCGC 1 cut(s) 302
SspI AATATT 1 cut(s) 433
SspMI CTAG 3 cut(s) 105, 344, 482
StyD4I CCNGG 1 cut(s) 243
TaaI ACNGT 1 cut(s) 407
TaiI ACGT 1 cut(s) 71
TaqI TCGA 2 cut(s) 33, 74
TasI AATT 2 cut(s) 297, 494
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TseFI GTSAC 1 cut(s) 64
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 2 cut(s) 231, 344
VpaK11BI GGWCC 1 cut(s) 247
XbaI TCTAGA 1 cut(s) 343
XspI CTAG 3 cut(s) 105, 344, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.