MD08G1197300.v1.1
MADS Family

MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000389
Physical Location & Seq
Forward (+)
25573461 .. 25585491
12031 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1197300.v1.1.491

Sequence Viewer

Length: 693 bp
ATGAATGAGAAGATTAAGATCAGGAGGATCGACTACTTGCCGGCAAGGCAGGTGACCTTCTCAAAGAGGAGAAGAGGGATTTTCAAGAAAGCTGAAGAGCTGTCGATTCTGTGTGAATCTGAAGTTGCTGTTATCATCTTTTCTCAAACTGGCAAGCTCTTTGATTACTCAAGCACCAGTACCAAGGATGTGATTGCAAGGTACAAATCACATACTGGTGGGGAAAAATCGGATCAAATCACGCTTCACCAACTGCAGTCGGAGAAAGAAAACACGATCAGGCTGAGTAAGGAACTTGAGGATAAGACCCGCAAGCTGAGGCATATGAAGGGTGAGGACCTTCAAGACTTGGATCTGGATCAACTGAACAAGTTAGAAAAATTGGTGGAAGTAAGCATTGGCCGTGTAATAAAAACTAAGGAAAAAAAGATAATGAGTGAGATTATGGCACTTACGAACAAGGGAGCTGAGCTTATAGAAGCTAACAACCAACTAAAGCAGAGGTTGGTGATGTTATCCGCTGGAGGAGATATCGAACCGGCGGCGATCATGGAGTTGGAAAACCTGAATAATGTTGGAGAAGAAGGCATGACATCTGAATCAGCCACAAATGTCACCGCCTGCTCCAGCAGTGCTCTTTCTCTCGAAGATGACTGCTCCGACATCTTGTCTCTCAAACTGGGGCTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000060 GO:0000900 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006417 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0009266 GO:0009628 GO:0009719 GO:0009725 GO:0009739 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009908 GO:0009909 GO:0009910 GO:0010033 GO:0010073 GO:0010074 GO:0010077 GO:0010219 GO:0010220 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0015031 GO:0015833 GO:0017038 GO:0017148 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0030371 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0033993 GO:0034248 GO:0034249 GO:0034504 GO:0034613 GO:0040034 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045182 GO:0045184 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046907 GO:0046982 GO:0046983 GO:0048367 GO:0048438 GO:0048506 GO:0048507 GO:0048509 GO:0048510 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051169 GO:0051170 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051641 GO:0051649 GO:0060255 GO:0061458 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0080090 GO:0080134 GO:0090079 GO:0090567 GO:0097159 GO:0098727 GO:0140110 GO:1901363 GO:1901700 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04022 ko04371 ko05418 map04013 map04022 map04371 map05418
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

231

Amino Acids

25.77

Weight (kDa)

6.05

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 15 - 56 1.4e-22 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 84 - 169 3.4e-13 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000389)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03630 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_5g35400 FvH4_5g35400 FvH4_5g35400 FvH4_5g35400 FvH4_5g35401 FvH4_5g35401
malus_domestica MD08G1196900.v1.1 MD08G1197000.v1.1 MD08G1197200.v1.1 MD08G1197300.v1.1 MD15G1384500.v1.1 MD15G1384600.v1.1
prunus_persica Prupe.1G531100_v2.0.a1 Prupe.1G531100_v2.0.a1 Prupe.1G531400_v2.0.a1 Prupe.1G531600_v2.0.a1 Prupe.1G531700_v2.0.a1
pyrus_communis pycom08g16910 pycom08g16940 pycom08g16960 pycom15g34480 pycom15g34490
rosa_chinensis RchiOBHm_Chr4g0434911 RchiOBHm_Chr7g0205051 RchiOBHm_Chr7g0205061 RchiOBHm_Chr7g0205071 RchiOBHm_Chr7g0236801 RchiOBHm_Chr7g0236811
rosa_laevigata RLG00000001060 RLG00000003404 RLG00000003405 RLG00000006636
rosa_multiflora Rmu_sc0008447.1_g000001 Rmu_sc0008851.1_g000011 Rmu_sc0008851.1_g000015
rosa_roxburghii Rroxscaffold_3G00224810 Rroxscaffold_3G00224820 Rroxscaffold_3G00252600 Rroxscaffold_5G00376120
rosa_rugosa Rorug04G0280500 Rorug04G0280600 Rorug04G0280700 Rorug04G0280800 Rorug04G0280900 Rorug04G0281000 Rorug04G0281100 Rorug07G0087100 Rorug07G0087200 Rorug07G0087200 Rorug07G0087300 Rorug07G0299300 Rorug07G0299400 Rorug07G0299500
rosa_samantha Rh4AG336000 Rh4BG344500 Rh4CG358900 Rh4DG338500 Rh7AG218200 Rh7AG218300 Rh7AG454500 Rh7AG454700 Rh7BG214900 Rh7BG224100 Rh7BG425700 Rh7BG425800 Rh7BG425900 Rh7CG231500 Rh7CG473200 Rh7CG473300 Rh7DG225600 Rh7DG225800 Rh7DG442500 Rh7DG442600
rosa_wichuraiana Rw4G029320 Rw7G018890 Rw7G018930 Rw7G037730 Rw7G037740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 40
Acc36I ACCTGC 1 cut(s) 40
AciI CCGC 4 cut(s) 310, 519, 542, 618
AclWI GGATC 4 cut(s) 35, 240, 360, 366
AcoI YGGCCR 1 cut(s) 400
AcuI CTGAAG 2 cut(s) 114, 141
AfaI GTAC 2 cut(s) 181, 203
AgsI TTSAA 2 cut(s) 85, 344
AhdI GACNNNNNGTC 1 cut(s) 667
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
AluBI AGCT 7 cut(s) 92, 100, 157, 316, 467, 472, 482
AluI AGCT 7 cut(s) 92, 100, 157, 316, 467, 472, 482
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 1 cut(s) 675
AlwI GGATC 4 cut(s) 35, 240, 360, 366
AoxI GGCC 1 cut(s) 400
AspS9I GGNCC 1 cut(s) 337
AsuHPI GGTGA 5 cut(s) 64, 239, 344, 520, 607
AvaII GGWCC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 637
BbvCI CCTCAGC 1 cut(s) 317
BceAI ACGGC 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 675
BfmI CTRYAG 1 cut(s) 254
BfuAI ACCTGC 1 cut(s) 40
BglI GCCNNNNNGGC 1 cut(s) 46
BisI GCNGC 1 cut(s) 543
BlpI GCTNAGC 1 cut(s) 468
BlsI GCNGC 1 cut(s) 544
Bme18I GGWCC 1 cut(s) 337
BmeRI GACNNNNNGTC 1 cut(s) 667
BmgT120I GGNCC 1 cut(s) 337
BmrI ACTGGG 1 cut(s) 689
BmuI ACTGGG 1 cut(s) 689
BpmI CTGGAG 2 cut(s) 543, 610
Bpu10I CCTNAGC 1 cut(s) 317
Bpu1102I GCTNAGC 1 cut(s) 468
BpuEI CTTGAG 2 cut(s) 154, 317
BsaBI GATNNNNATC 2 cut(s) 17, 357
BsaJI CCNNGG 1 cut(s) 183
Bse118I RCCGGY 2 cut(s) 40, 538
Bse1I ACTGG 4 cut(s) 154, 177, 220, 684
Bse8I GATNNNNATC 2 cut(s) 17, 357
BseDI CCNNGG 1 cut(s) 183
BseGI GGATG 1 cut(s) 193
BseJI GATNNNNATC 2 cut(s) 17, 357
BseMII CTCAG 3 cut(s) 275, 308, 459
BseNI ACTGG 4 cut(s) 154, 177, 220, 684
BseRI GAGGAG 2 cut(s) 82, 540
BshFI GGCC 1 cut(s) 402
BsiHKAI GWGCWC 1 cut(s) 637
BsiSI CCGG 2 cut(s) 41, 539
BsmAI GTCTC 1 cut(s) 675
BsnI GGCC 1 cut(s) 402
Bsp1286I GDGCHC 1 cut(s) 637
Bsp143I GATC 7 cut(s) 18, 27, 232, 276, 352, 358, 546
Bsp1720I GCTNAGC 1 cut(s) 468
BspACI CCGC 4 cut(s) 310, 519, 542, 618
BspANI GGCC 1 cut(s) 402
BspCNI CTCAG 3 cut(s) 276, 309, 460
BspMAI CTGCAG 1 cut(s) 258
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 4 cut(s) 35, 240, 360, 366
BspQI GCTCTTC 1 cut(s) 90
BsrFI RCCGGY 2 cut(s) 40, 538
BsrI ACTGG 4 cut(s) 154, 177, 220, 684
BssAI RCCGGY 2 cut(s) 40, 538
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 7 cut(s) 18, 27, 232, 276, 352, 358, 546
BssT1I CCWWGG 1 cut(s) 183
Bst6I CTCTTC 2 cut(s) 67, 90
BstC8I GCNNGC 4 cut(s) 42, 155, 314, 622
BstDEI CTNAG 5 cut(s) 284, 317, 417, 468, 690
BstEII GGTNACC 1 cut(s) 52
BstF5I GGATG 1 cut(s) 193
BstKTI GATC 7 cut(s) 21, 30, 235, 279, 355, 361, 549
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 7 cut(s) 18, 27, 232, 276, 352, 358, 546
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstPI GGTNACC 1 cut(s) 52
BstSFI CTRYAG 1 cut(s) 254
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 1 cut(s) 402
BtsCI GGATG 1 cut(s) 193
BtsI GCAGTG 1 cut(s) 637
BtsIMutI CAGTG 1 cut(s) 637
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 4 cut(s) 42, 155, 314, 622
Cfr10I RCCGGY 2 cut(s) 40, 538
Cfr13I GGNCC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 180, 202
CviAII CATG 2 cut(s) 550, 589
CviQI GTAC 2 cut(s) 180, 202
DdeI CTNAG 5 cut(s) 284, 317, 417, 468, 690
DpnI GATC 7 cut(s) 20, 29, 234, 278, 354, 360, 548
DpnII GATC 7 cut(s) 18, 27, 232, 276, 352, 358, 546
DriI GACNNNNNGTC 1 cut(s) 667
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 2 cut(s) 67, 90
Eam1105I GACNNNNNGTC 1 cut(s) 667
EarI CTCTTC 2 cut(s) 67, 90
Eco130I CCWWGG 1 cut(s) 183
Eco32I GATATC 1 cut(s) 532
Eco47I GGWCC 1 cut(s) 337
Eco57I CTGAAG 2 cut(s) 114, 141
Eco91I GGTNACC 1 cut(s) 52
EcoO109I RGGNCCY 1 cut(s) 337
EcoO65I GGTNACC 1 cut(s) 52
EcoRV GATATC 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 2 cut(s) 553, 592
FaiI YATR 7 cut(s) 213, 324, 326, 446, 476, 551, 590
FatI CATG 2 cut(s) 549, 588
FauI CCCGC 1 cut(s) 317
FauNDI CATATG 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 543
FokI GGATG 1 cut(s) 200
Fsp4HI GCNGC 1 cut(s) 543
GluI GCNGC 1 cut(s) 543
GsuI CTGGAG 2 cut(s) 543, 610
HaeIII GGCC 1 cut(s) 402
HapII CCGG 2 cut(s) 41, 539
Hin1II CATG 2 cut(s) 553, 592
HinfI GANTC 3 cut(s) 106, 116, 599
HpaII CCGG 2 cut(s) 41, 539
HphI GGTGA 5 cut(s) 64, 239, 344, 520, 607
Hpy188I TCNGA 5 cut(s) 121, 232, 262, 598, 661
Hpy188III TCNNGA 5 cut(s) 22, 85, 344, 356, 644
HpyAV CCTTC 4 cut(s) 67, 322, 350, 578
HpyCH4V TGCA 2 cut(s) 197, 256
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
HpyF3I CTNAG 5 cut(s) 284, 317, 417, 468, 690
Hsp92II CATG 2 cut(s) 553, 592
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Kzo9I GATC 7 cut(s) 18, 27, 232, 276, 352, 358, 546
LguI GCTCTTC 1 cut(s) 90
LmnI GCTCC 3 cut(s) 464, 629, 662
MaeIII GTNAC 2 cut(s) 52, 613
MalI GATC 7 cut(s) 20, 29, 234, 278, 354, 360, 548
MboI GATC 7 cut(s) 18, 27, 232, 276, 352, 358, 546
MboII GAAGA 5 cut(s) 22, 84, 107, 593, 659
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 1 cut(s) 637
MluCI AATT 1 cut(s) 380
MmeI TCCRAC 4 cut(s) 240, 537, 556, 684
MnlI CCTC 8 cut(s) 18, 60, 68, 292, 312, 328, 495, 518
MroNI GCCGGC 1 cut(s) 40
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 1 cut(s) 216
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 2 cut(s) 41, 539
MwoI GCNNNNNNNGC 1 cut(s) 46
NaeI GCCGGC 1 cut(s) 42
NdeI CATATG 1 cut(s) 324
NdeII GATC 7 cut(s) 18, 27, 232, 276, 352, 358, 546
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 2 cut(s) 553, 592
NmuCI GTSAC 2 cut(s) 52, 613
PaqCI CACCTGC 1 cut(s) 40
PciSI GCTCTTC 1 cut(s) 90
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 3 cut(s) 106, 116, 599
PkrI GCNGC 1 cut(s) 544
PpuMI RGGWCCY 1 cut(s) 337
Psp5II RGGWCCY 1 cut(s) 337
PspEI GGTNACC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 337
PspPPI RGGWCCY 1 cut(s) 337
PstI CTGCAG 1 cut(s) 258
PsuI RGATCY 1 cut(s) 352
RsaI GTAC 2 cut(s) 181, 203
RsaNI GTAC 2 cut(s) 180, 202
RseI CAYNNNNRTG 1 cut(s) 216
SapI GCTCTTC 1 cut(s) 90
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 1 cut(s) 543
Sau3AI GATC 7 cut(s) 18, 27, 232, 276, 352, 358, 546
Sau96I GGNCC 1 cut(s) 337
SduI GDGCHC 1 cut(s) 637
SfcI CTRYAG 1 cut(s) 254
SinI GGWCC 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 216
SmlI CTYRAG 2 cut(s) 169, 296
SmoI CTYRAG 2 cut(s) 169, 296
Sse9I AATT 1 cut(s) 380
SsiI CCGC 4 cut(s) 310, 519, 542, 618
StyI CCWWGG 1 cut(s) 183
TaqI TCGA 4 cut(s) 30, 104, 534, 645
TasI AATT 1 cut(s) 380
TauI GCSGC 1 cut(s) 545
TfiI GAWTC 3 cut(s) 106, 116, 599
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 1 cut(s) 637
TseFI GTSAC 2 cut(s) 52, 613
Tsp45I GTSAC 2 cut(s) 52, 613
TspDTI ATGAA 2 cut(s) 17, 341
TspRI CASTG 1 cut(s) 637
VpaK11BI GGWCC 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.