FvH4_5g35401
MADS Family

MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000389
Physical Location & Seq
Reverse (-)
25938753 .. 25946338
7586 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35401.t2

Sequence Viewer

Length: 579 bp
ATGGCTTCGGAGTTCAGACTTAGTATGAGATTTTTATTTTTTATTTTCCCTAGAAACTGCACGGTGGATGTCATTGCAAGGTTCAAACTGTGCACTGAAGATGTGGAAGAGGGGGACCAGCAGTCACCTAATGAGCTCCAGTTGGTGACTGATGAGTGCATGAGGTTGAATATGGAGCTTGCGGAAAAGAACCACAAGCTAAGGCATATGGAGGGGCAGGATCTGGAAGAGCTGAAAATAGATGAGTTGCAGAGATTGGAGAATATGATTGAAGGAGGACTTAGCCGCGTACTTCAAACTAAGGATCAGAGGATTATGAGTCAGATTCTGGCACTTGAAACAAAGGGAGCAGAGTTGACAGAAGCAAACAACCTATTAAGGCAGAGGTTAGGAACGCTATCAAATGGAGATGGAAATAAAGCTAGTGGCGTCCTTTCGGATCCGGGGATCTCAACTGATGAAGAAGATATGAGATCAGGATCTTGCACAATTGCCACCAGCTGTTTTAGTACTGGCTCTTCGAGTTCGTCCATGGATGACTCCTCCTCTGAGTACACCTTGTCTCTCAAACTTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000060 GO:0000900 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006417 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0009266 GO:0009628 GO:0009719 GO:0009725 GO:0009739 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009908 GO:0009909 GO:0009910 GO:0010033 GO:0010073 GO:0010074 GO:0010077 GO:0010219 GO:0010220 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0015031 GO:0015833 GO:0017038 GO:0017148 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0030371 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0033993 GO:0034248 GO:0034249 GO:0034504 GO:0034613 GO:0040034 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045182 GO:0045184 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046907 GO:0046982 GO:0046983 GO:0048367 GO:0048438 GO:0048506 GO:0048507 GO:0048509 GO:0048510 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051169 GO:0051170 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051641 GO:0051649 GO:0060255 GO:0061458 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0080090 GO:0080134 GO:0090079 GO:0090567 GO:0097159 GO:0098727 GO:0140110 GO:1901363 GO:1901700 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04022 ko04371 ko05418 map04013 map04022 map04371 map05418
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

193

Amino Acids

21.4

Weight (kDa)

4.61

Isoelectric Point (pI)

61.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 55 - 118 3.9e-14 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000389)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03630 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27110 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_4g27440 FvH4_5g35400 FvH4_5g35400 FvH4_5g35400 FvH4_5g35400 FvH4_5g35401 FvH4_5g35401
malus_domestica MD08G1196900.v1.1 MD08G1197000.v1.1 MD08G1197200.v1.1 MD08G1197300.v1.1 MD15G1384500.v1.1 MD15G1384600.v1.1
prunus_persica Prupe.1G531100_v2.0.a1 Prupe.1G531100_v2.0.a1 Prupe.1G531400_v2.0.a1 Prupe.1G531600_v2.0.a1 Prupe.1G531700_v2.0.a1
pyrus_communis pycom08g16910 pycom08g16940 pycom08g16960 pycom15g34480 pycom15g34490
rosa_chinensis RchiOBHm_Chr4g0434911 RchiOBHm_Chr7g0205051 RchiOBHm_Chr7g0205061 RchiOBHm_Chr7g0205071 RchiOBHm_Chr7g0236801 RchiOBHm_Chr7g0236811
rosa_laevigata RLG00000001060 RLG00000003404 RLG00000003405 RLG00000006636
rosa_multiflora Rmu_sc0008447.1_g000001 Rmu_sc0008851.1_g000011 Rmu_sc0008851.1_g000015
rosa_roxburghii Rroxscaffold_3G00224810 Rroxscaffold_3G00224820 Rroxscaffold_3G00252600 Rroxscaffold_5G00376120
rosa_rugosa Rorug04G0280500 Rorug04G0280600 Rorug04G0280700 Rorug04G0280800 Rorug04G0280900 Rorug04G0281000 Rorug04G0281100 Rorug07G0087100 Rorug07G0087200 Rorug07G0087200 Rorug07G0087300 Rorug07G0299300 Rorug07G0299400 Rorug07G0299500
rosa_samantha Rh4AG336000 Rh4BG344500 Rh4CG358900 Rh4DG338500 Rh7AG218200 Rh7AG218300 Rh7AG454500 Rh7AG454700 Rh7BG214900 Rh7BG224100 Rh7BG425700 Rh7BG425800 Rh7BG425900 Rh7CG231500 Rh7CG473200 Rh7CG473300 Rh7DG225600 Rh7DG225800 Rh7DG442500 Rh7DG442600
rosa_wichuraiana Rw4G029320 Rw7G018890 Rw7G018930 Rw7G037730 Rw7G037740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 288
AciI CCGC 2 cut(s) 182, 286
AclWI GGATC 6 cut(s) 228, 312, 434, 447, 455, 487
AcuI CTGAAG 1 cut(s) 117
AcyI GRCGYC 1 cut(s) 429
AfaI GTAC 3 cut(s) 291, 511, 554
AgsI TTSAA 5 cut(s) 85, 169, 272, 296, 338
AhdI GACNNNNNGTC 1 cut(s) 121
AluBI AGCT 6 cut(s) 136, 178, 199, 232, 422, 501
AluI AGCT 6 cut(s) 136, 178, 199, 232, 422, 501
Alw21I GWGCWC 2 cut(s) 95, 138
Alw26I GTCTC 1 cut(s) 567
Alw44I GTGCAC 1 cut(s) 91
AlwI GGATC 6 cut(s) 228, 312, 434, 447, 455, 487
AlwNI CAGNNNCTG 2 cut(s) 223, 328
ApaLI GTGCAC 1 cut(s) 91
AspS9I GGNCC 1 cut(s) 115
AsuC2I CCSGG 1 cut(s) 444
AsuHPI GGTGA 2 cut(s) 117, 157
AvaII GGWCC 1 cut(s) 115
BaeGI GKGCMC 1 cut(s) 95
BamHI GGATCC 1 cut(s) 439
BanII GRGCYC 1 cut(s) 138
BarI GAAGNNNNNNTAC 2 cut(s) 502, 534
Bbv12I GWGCWC 2 cut(s) 95, 138
BccI CCATC 1 cut(s) 404
BcnI CCSGG 1 cut(s) 444
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 2 cut(s) 51, 423
BisI GCNGC 1 cut(s) 286
BlsI GCNGC 1 cut(s) 287
BmcAI AGTACT 1 cut(s) 511
Bme1390I CCNGG 1 cut(s) 444
Bme18I GGWCC 1 cut(s) 115
BmeRI GACNNNNNGTC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 2 cut(s) 116, 441
BmrFI CCNGG 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 122
Bpu10I CCTNAGC 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 444
BsaBI GATNNNNATC 1 cut(s) 478
BsaHI GRCGYC 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 443, 531
Bse1I ACTGG 2 cut(s) 139, 517
Bse3DI GCAATG 1 cut(s) 72
Bse8I GATNNNNATC 1 cut(s) 478
BseDI CCNNGG 2 cut(s) 443, 531
BseGI GGATG 2 cut(s) 73, 541
BseJI GATNNNNATC 1 cut(s) 478
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 2 cut(s) 139, 517
BseRI GAGGAG 2 cut(s) 532, 535
BseSI GKGCMC 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 288
BsiHKAI GWGCWC 2 cut(s) 95, 138
BsiSI CCGG 1 cut(s) 443
BslFI GGGAC 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 567
BsmFI GGGAC 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 95, 138
Bsp143I GATC 6 cut(s) 220, 304, 439, 447, 473, 479
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 2 cut(s) 182, 286
BspCNI CTCAG 1 cut(s) 541
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 116, 441
BspPI GGATC 6 cut(s) 228, 312, 434, 447, 455, 487
BspQI GCTCTTC 2 cut(s) 222, 523
BsrDI GCAATG 1 cut(s) 72
BsrI ACTGG 2 cut(s) 139, 517
BssECI CCNNGG 2 cut(s) 443, 531
BssMI GATC 6 cut(s) 220, 304, 439, 447, 473, 479
BssNI GRCGYC 1 cut(s) 429
BssT1I CCWWGG 1 cut(s) 531
Bst4CI ACNGT 2 cut(s) 64, 90
Bst6I CTCTTC 3 cut(s) 102, 222, 523
BstACI GRCGYC 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 5 cut(s) 20, 200, 281, 300, 549
BstDSI CCRYGG 1 cut(s) 531
BstF5I GGATG 2 cut(s) 73, 541
BstFNI CGCG 1 cut(s) 288
BstKTI GATC 6 cut(s) 223, 307, 442, 450, 476, 482
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 6 cut(s) 220, 304, 439, 447, 473, 479
BstSCI CCNGG 1 cut(s) 442
BstSLI GKGCMC 1 cut(s) 95
BstUI CGCG 1 cut(s) 288
BstX2I RGATCY 4 cut(s) 220, 439, 447, 479
BstYI RGATCY 4 cut(s) 220, 439, 447, 479
BtgI CCRYGG 1 cut(s) 531
BtsCI GGATG 2 cut(s) 73, 541
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 1 cut(s) 180
CaiI CAGNNNCTG 2 cut(s) 223, 328
Cfr13I GGNCC 1 cut(s) 115
CseI GACGC 1 cut(s) 418
Csp6I GTAC 3 cut(s) 290, 510, 553
CviAII CATG 2 cut(s) 160, 532
CviJI RGCY 9 cut(s) 5, 136, 178, 199, 232, 285, 422, 501, 516
CviKI_1 RGCY 9 cut(s) 5, 136, 178, 199, 232, 285, 422, 501, 516
CviQI GTAC 3 cut(s) 290, 510, 553
DdeI CTNAG 5 cut(s) 20, 200, 281, 300, 549
DpnI GATC 6 cut(s) 222, 306, 441, 449, 475, 481
DpnII GATC 6 cut(s) 220, 304, 439, 447, 473, 479
DriI GACNNNNNGTC 1 cut(s) 121
Eam1104I CTCTTC 3 cut(s) 102, 222, 523
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 3 cut(s) 102, 222, 523
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 531
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 115
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 531
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 2 cut(s) 163, 535
FaiI YATR 9 cut(s) 26, 161, 173, 207, 209, 266, 317, 470, 533
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FaqI GGGAC 1 cut(s) 128
FatI CATG 2 cut(s) 159, 531
FauNDI CATATG 1 cut(s) 207
Fnu4HI GCNGC 1 cut(s) 286
FokI GGATG 2 cut(s) 80, 548
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 1 cut(s) 286
FspBI CTAG 2 cut(s) 51, 423
GluI GCNGC 1 cut(s) 286
GsuI CTGGAG 1 cut(s) 122
HapII CCGG 1 cut(s) 443
HgaI GACGC 1 cut(s) 418
Hin1I GRCGYC 1 cut(s) 429
Hin1II CATG 2 cut(s) 163, 535
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 3 cut(s) 319, 325, 539
HpaII CCGG 1 cut(s) 443
HphI GGTGA 2 cut(s) 117, 157
Hpy166II GTNNAC 3 cut(s) 93, 357, 555
Hpy188I TCNGA 6 cut(s) 10, 17, 309, 324, 439, 550
Hpy188III TCNNGA 2 cut(s) 224, 477
Hpy8I GTNNAC 3 cut(s) 93, 357, 555
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 2 cut(s) 64, 90
HpyCH4V TGCA 6 cut(s) 60, 77, 93, 159, 250, 486
HpyF3I CTNAG 5 cut(s) 20, 200, 281, 300, 549
Hsp92I GRCGYC 1 cut(s) 429
Hsp92II CATG 2 cut(s) 163, 535
Kzo9I GATC 6 cut(s) 220, 304, 439, 447, 473, 479
LguI GCTCTTC 2 cut(s) 222, 523
LmnI GCTCC 3 cut(s) 141, 175, 347
LpnPI CCDG 9 cut(s) 131, 152, 203, 209, 314, 456, 462, 498, 511
MaeI CTAG 2 cut(s) 51, 423
MaeIII GTNAC 2 cut(s) 123, 145
MalI GATC 6 cut(s) 222, 306, 441, 449, 475, 481
MboI GATC 6 cut(s) 220, 304, 439, 447, 473, 479
MboII GAAGA 6 cut(s) 110, 119, 239, 473, 476, 510
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 4 cut(s) 220, 439, 447, 479
MhlI GDGCHC 2 cut(s) 95, 138
MluCI AATT 1 cut(s) 489
MlyI GAGTC 2 cut(s) 328, 533
MnlI CCTC 8 cut(s) 103, 156, 205, 269, 303, 378, 553, 556
MseI TTAA 1 cut(s) 377
MspA1I CMGCKG 1 cut(s) 501
MspI CCGG 1 cut(s) 443
MspR9I CCNGG 1 cut(s) 444
MunI CAATTG 1 cut(s) 489
MvnI CGCG 1 cut(s) 288
NciI CCSGG 1 cut(s) 444
NcoI CCATGG 1 cut(s) 531
NdeI CATATG 1 cut(s) 207
NdeII GATC 6 cut(s) 220, 304, 439, 447, 473, 479
NlaIII CATG 2 cut(s) 163, 535
NlaIV GGNNCC 2 cut(s) 116, 441
NmuCI GTSAC 2 cut(s) 123, 145
PciSI GCTCTTC 2 cut(s) 222, 523
PfeI GAWTC 1 cut(s) 325
PkrI GCNGC 1 cut(s) 287
PleI GAGTC 2 cut(s) 327, 533
PpsI GAGTC 2 cut(s) 327, 533
Psp124BI GAGCTC 1 cut(s) 138
PspN4I GGNNCC 2 cut(s) 116, 441
PspPI GGNCC 1 cut(s) 115
PstNI CAGNNNCTG 2 cut(s) 223, 328
PsuI RGATCY 4 cut(s) 220, 439, 447, 479
PvuII CAGCTG 1 cut(s) 501
RsaI GTAC 3 cut(s) 291, 511, 554
RsaNI GTAC 3 cut(s) 290, 510, 553
SacI GAGCTC 1 cut(s) 138
SapI GCTCTTC 2 cut(s) 222, 523
SaqAI TTAA 1 cut(s) 377
SatI GCNGC 1 cut(s) 286
Sau3AI GATC 6 cut(s) 220, 304, 439, 447, 473, 479
Sau96I GGNCC 1 cut(s) 115
ScaI AGTACT 1 cut(s) 511
SchI GAGTC 2 cut(s) 328, 533
ScrFI CCNGG 1 cut(s) 444
SduI GDGCHC 2 cut(s) 95, 138
SinI GGWCC 1 cut(s) 115
Sse9I AATT 1 cut(s) 489
SsiI CCGC 2 cut(s) 182, 286
SspMI CTAG 2 cut(s) 51, 423
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 442
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 2 cut(s) 64, 90
TaqI TCGA 1 cut(s) 521
TasI AATT 1 cut(s) 489
TatI WGTACW 2 cut(s) 509, 552
TauI GCSGC 1 cut(s) 288
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 1 cut(s) 377
Tru9I TTAA 1 cut(s) 377
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 2 cut(s) 123, 145
Tsp45I GTSAC 2 cut(s) 123, 145
TspDTI ATGAA 1 cut(s) 474
TspRI CASTG 1 cut(s) 100
VneI GTGCAC 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 115
XspI CTAG 2 cut(s) 51, 423
ZrmI AGTACT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.