FvH4_5g32530
ERF Family

P-loop nucleoside triphosphate hydrolase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000615
Physical Location & Seq
Forward (+)
23467930 .. 23479727
11798 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32530.t2

Sequence Viewer

Length: 1008 bp
ATGGGTGGAAGTTCAATTGATGATGACTGGGAGCTTCCCTTTACTAATGCTGCTCGCACTGTGGTCTTAGTTGGACGCACTGGTAATGGAAAAAGTGCAACTGGAAACAGCATTCTTGGCAAAAAAGCCTTCAATTCCAAGCGGAGCTCTAATGGTGTCACTAGCACTTGTGAACTGCAGACTGCTACCTTGAGAGATGGACAAAAAGTCAATGTTATAGACACTCCTGGGCTTTTTGATTATTCTGCCAAGTCAGAGTTTATTGGCAAAGAAATTGTCAAATGCATTAATTTGGCCAAGGATGGGATCCATGCTGTTCTTGTGGTTTTCTCAACTCGAACTCGCTTTTCACAGGAAGAGGAGTCTGCAATCCGTAGCTTGCAAACTCTATTTGGAAGTAAAATTTTTGACTATATGATTGTTGTCTTTACGGGAGGAGATGACTTTGAAGAAAATGATGAGACTTTGGAGGATTATTTAGGCCGTGATTGCCCGAAGCCTTTAAAGGAAATTCTTGGTCTCTGCGGAAATCGCCGTGTGCTTTTTGATAACAAGACCAAGGATGAGAGCAAGAGGGTTGAACAAGTACAGCAGCTTCTCGCGCTTGTAAACTCGGTTTTAGCACATAATGGTGGGCGGCCATACTCGGATGAGATATTTGCAGAAGTGAAGAAAGGGGCTATAAAACTTCGTGATCAACAAGTAGAGGTTGCTTCTTTGAAGGGGTATTCAAAGACAGAAATATCTCATTTGAATGAGCAGATGCAGCGTGCACATGATCTGCAGCTTAAACGAATTACTGAGATGGTTGAGTCGAAGATGAGGGAGACCACCATGATGCTTGAACAAAAGTTAGCAGATGAACATGCTGCACGACTAAAAGCAGAAGAGACTGCCTTAGAGGCTCAACAAAGGTCTGAGGATCAAATCCGAAAGCTTAGAGATGATCTACAAAAAGCAGAGGAGGAGCTTCGGAAAAGAGGCGAAACCAAGTGTGCTATTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AIG1

Protein Analysis

336

Amino Acids

37.55

Weight (kDa)

5.91

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIG1 PF04548 19 - 234 2.2e-84 AIG1 family
MMR_HSR1 PF01926 20 - 127 8.2e-10 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000615)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33970 AT1G33970 AT1G33970 AT1G33970 AT1G33970
fragaria_vesca FvH4_5g32530 FvH4_5g32530
malus_domestica MD15G1403700.v1.1
prunus_persica Prupe.1G549700_v2.0.a1 Prupe.1G549700_v2.0.a1
pyrus_communis pycom15g36100
rosa_chinensis RchiOBHm_Chr7g0226331 RchiOBHm_Chr7g0229101 RchiOBHm_Chr7g0229111 RchiOBHm_Chr7g0229141 RchiOBHm_Chr7g0229161 RchiOBHm_Chr7g0229171 RchiOBHm_Chr7g0229211 RchiOBHm_Chr7g0229241 RchiOBHm_Chr7g0229381 RchiOBHm_Chr7g0229761 RchiOBHm_Chr7g0229911
rosa_laevigata RLG00000000796 RLG00000001464 RLG00000001466 RLG00000001469 RLG00000001523 RLG00000001526 RLG00000001529 RLG00000001530 RLG00000001749 RLG00000001913 RLG00000002114 RLG00000002115 RLG00000023248 RLG00000023250
rosa_multiflora Rmu_co8281135.1_g000001 Rmu_sc0000271.1_g000002 Rmu_sc0000271.1_g000007 Rmu_sc0000271.1_g000014 Rmu_sc0000693.1_g000012 Rmu_sc0000693.1_g000015 Rmu_sc0000693.1_g000023 Rmu_sc0001044.1_g000005 Rmu_sc0006689.1_g000007 Rmu_sc0012733.1_g000005
rosa_roxburghii Rroxscaffold_3G00230540 Rroxscaffold_3G00230590 Rroxscaffold_3G00230600 Rroxscaffold_3G00230700 Rroxscaffold_3G00230710 Rroxscaffold_3G00232980 Rroxscaffold_3G00237610
rosa_rugosa Rorug07G0203800 Rorug07G0203900 Rorug07G0256100 Rorug07G0256400 Rorug07G0256400 Rorug07G0256400 Rorug07G0256500 Rorug07G0256600 Rorug07G0256600 Rorug07G0257100 Rorug07G0257200 Rorug07G0257300 Rorug07G0257400
rosa_samantha Rh7DG401100 Rh7DG401300 Rh7DG401900 Rh7DG402100 Rh7DG402300 Rh7DG402700
rosa_wichuraiana Rw0G001910 Rw0G001930 Rw0G001940 Rw7G033790 Rw7G033810 Rw7G033840 Rw7G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 602
AciI CCGC 3 cut(s) 142, 525, 637
AclWI GGATC 3 cut(s) 301, 314, 930
AcoI YGGCCR 2 cut(s) 294, 638
AcsI RAATTY 2 cut(s) 402, 510
AfaI GTAC 1 cut(s) 588
AfiI CCNNNNNNNGG 1 cut(s) 303
AgsI TTSAA 8 cut(s) 15, 133, 449, 581, 721, 732, 754, 845
AhdI GACNNNNNGTC 1 cut(s) 206
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 7 cut(s) 34, 147, 378, 595, 787, 937, 970
AluI AGCT 7 cut(s) 34, 147, 378, 595, 787, 937, 970
Alw21I GWGCWC 2 cut(s) 149, 775
Alw26I GTCTC 4 cut(s) 455, 524, 821, 884
Alw44I GTGCAC 1 cut(s) 771
AlwI GGATC 3 cut(s) 301, 314, 930
AoxI GGCC 3 cut(s) 294, 481, 638
ApaLI GTGCAC 1 cut(s) 771
ApeKI GCWGC 5 cut(s) 50, 592, 766, 784, 869
ApoI RAATTY 2 cut(s) 402, 510
ArsI GACNNNNNNTTYG 2 cut(s) 261, 293
AseI ATTAAT 1 cut(s) 288
AspLEI GCGC 1 cut(s) 604
BaeGI GKGCMC 1 cut(s) 775
BalI TGGCCA 1 cut(s) 296
BamHI GGATCC 1 cut(s) 306
BanII GRGCYC 1 cut(s) 149
BarI GAAGNNNNNNTAC 2 cut(s) 579, 611
Bbv12I GWGCWC 2 cut(s) 149, 775
BbvI GCAGC 5 cut(s) 37, 604, 778, 796, 856
BccI CCATC 3 cut(s) 191, 296, 799
BceAI ACGGC 2 cut(s) 468, 519
BciT130I CCWGG 1 cut(s) 228
BclI TGATCA 1 cut(s) 694
BcoDI GTCTC 4 cut(s) 455, 524, 821, 884
BfaI CTAG 1 cut(s) 162
BfmI CTRYAG 2 cut(s) 176, 782
BglI GCCNNNNNGGC 1 cut(s) 902
BisI GCNGC 6 cut(s) 51, 593, 638, 767, 785, 870
BlsI GCNGC 6 cut(s) 52, 594, 639, 768, 786, 871
Bme1390I CCNGG 1 cut(s) 228
BmeRI GACNNNNNGTC 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 1 cut(s) 228
BmrI ACTGGG 1 cut(s) 37
BmsI GCATC 2 cut(s) 753, 828
BmuI ACTGGG 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 211
BsaI GGTCTC 2 cut(s) 524, 821
BsaJI CCNNGG 3 cut(s) 227, 297, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse1I ACTGG 3 cut(s) 32, 85, 106
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 227, 297, 558
BseGI GGATG 3 cut(s) 307, 568, 655
BseLI CCNNNNNNNGG 1 cut(s) 303
BseMII CTCAG 2 cut(s) 792, 909
BseNI ACTGG 3 cut(s) 32, 85, 106
BseRI GAGGAG 4 cut(s) 374, 450, 977, 980
BseSI GKGCMC 1 cut(s) 775
BseXI GCAGC 5 cut(s) 37, 604, 778, 796, 856
BsgI GTGCAG 1 cut(s) 855
Bsh1236I CGCG 1 cut(s) 602
BshFI GGCC 3 cut(s) 296, 483, 640
BsiHKAI GWGCWC 2 cut(s) 149, 775
BslI CCNNNNNNNGG 1 cut(s) 303
BsmAI GTCTC 4 cut(s) 455, 524, 821, 884
BsmI GAATGC 1 cut(s) 111
BsnI GGCC 3 cut(s) 296, 483, 640
Bso31I GGTCTC 2 cut(s) 524, 821
Bsp1286I GDGCHC 2 cut(s) 149, 775
Bsp143I GATC 5 cut(s) 306, 694, 778, 922, 946
BspACI CCGC 3 cut(s) 142, 525, 637
BspANI GGCC 3 cut(s) 296, 483, 640
BspCNI CTCAG 2 cut(s) 793, 910
BspFNI CGCG 1 cut(s) 602
BspLI GGNNCC 1 cut(s) 308
BspMAI CTGCAG 2 cut(s) 180, 786
BspPI GGATC 3 cut(s) 301, 314, 930
BspTNI GGTCTC 2 cut(s) 524, 821
BsrI ACTGG 3 cut(s) 32, 85, 106
BssECI CCNNGG 3 cut(s) 227, 297, 558
BssMI GATC 5 cut(s) 306, 694, 778, 922, 946
BssT1I CCWWGG 2 cut(s) 297, 558
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 61
Bst6I CTCTTC 2 cut(s) 351, 882
BstC8I GCNNGC 3 cut(s) 55, 380, 771
BstDEI CTNAG 5 cut(s) 67, 801, 898, 918, 938
BstF5I GGATG 3 cut(s) 307, 568, 655
BstFNI CGCG 1 cut(s) 602
BstHHI GCGC 1 cut(s) 604
BstKTI GATC 5 cut(s) 309, 697, 781, 925, 949
BstMAI GTCTC 4 cut(s) 455, 524, 821, 884
BstMBI GATC 5 cut(s) 306, 694, 778, 922, 946
BstMWI GCNNNNNNNGC 6 cut(s) 117, 489, 531, 601, 766, 902
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 869
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 2 cut(s) 176, 782
BstSLI GKGCMC 1 cut(s) 775
BstUI CGCG 1 cut(s) 602
BstV1I GCAGC 5 cut(s) 37, 604, 778, 796, 856
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
BsuRI GGCC 3 cut(s) 296, 483, 640
BtsCI GGATG 3 cut(s) 307, 568, 655
BtsIMutI CAGTG 2 cut(s) 57, 78
Cac8I GCNNGC 3 cut(s) 55, 380, 771
CfoI GCGC 1 cut(s) 604
CseI GACGC 1 cut(s) 84
Csp6I GTAC 1 cut(s) 587
CviAII CATG 4 cut(s) 311, 776, 835, 866
CviQI GTAC 1 cut(s) 587
DdeI CTNAG 5 cut(s) 67, 801, 898, 918, 938
DpnI GATC 5 cut(s) 308, 696, 780, 924, 948
DpnII GATC 5 cut(s) 306, 694, 778, 922, 946
DraI TTTAAA 1 cut(s) 504
DriI GACNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 2 cut(s) 294, 638
Eam1104I CTCTTC 2 cut(s) 351, 882
Eam1105I GACNNNNNGTC 1 cut(s) 206
EarI CTCTTC 2 cut(s) 351, 882
Ecl136II GAGCTC 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 297, 558
Eco24I GRGCYC 1 cut(s) 149
Eco31I GGTCTC 2 cut(s) 524, 821
Eco53kI GAGCTC 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 2 cut(s) 297, 558
EcoT22I ATGCAT 1 cut(s) 287
EcoT38I GRGCYC 1 cut(s) 149
ErhI CCWWGG 2 cut(s) 297, 558
FaeI CATG 4 cut(s) 314, 779, 838, 869
FatI CATG 4 cut(s) 310, 775, 834, 865
FbaI TGATCA 1 cut(s) 694
Fnu4HI GCNGC 6 cut(s) 51, 593, 638, 767, 785, 870
FokI GGATG 3 cut(s) 314, 575, 662
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 6 cut(s) 51, 593, 638, 767, 785, 870
FspBI CTAG 1 cut(s) 162
GlaI GCGC 1 cut(s) 603
GluI GCNGC 6 cut(s) 51, 593, 638, 767, 785, 870
HaeIII GGCC 3 cut(s) 296, 483, 640
HgaI GACGC 1 cut(s) 84
HhaI GCGC 1 cut(s) 604
Hin1II CATG 4 cut(s) 314, 779, 838, 869
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HindIII AAGCTT 1 cut(s) 935
HinfI GANTC 2 cut(s) 362, 812
Hpy166II GTNNAC 3 cut(s) 173, 610, 773
Hpy188I TCNGA 5 cut(s) 256, 649, 919, 932, 975
Hpy188III TCNNGA 1 cut(s) 692
Hpy8I GTNNAC 3 cut(s) 173, 610, 773
HpyAV CCTTC 2 cut(s) 139, 715
HpyCH4III ACNGT 1 cut(s) 61
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 489, 531, 601, 766, 902
HpyF3I CTNAG 5 cut(s) 67, 801, 898, 918, 938
Hsp92II CATG 4 cut(s) 314, 779, 838, 869
HspAI GCGC 1 cut(s) 602
Ksp22I TGATCA 1 cut(s) 694
Kzo9I GATC 5 cut(s) 306, 694, 778, 922, 946
LmnI GCTCC 3 cut(s) 31, 144, 967
LpnPI CCDG 6 cut(s) 13, 66, 87, 213, 240, 338
Lsp1109I GCAGC 5 cut(s) 37, 604, 778, 796, 856
LweI GCATC 2 cut(s) 753, 828
MaeI CTAG 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 157
MalI GATC 5 cut(s) 308, 696, 780, 924, 948
MboI GATC 5 cut(s) 306, 694, 778, 922, 946
MboII GAAGA 5 cut(s) 368, 461, 682, 829, 899
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 2 cut(s) 149, 775
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 7 cut(s) 15, 133, 273, 289, 402, 510, 795
MluNI TGGCCA 1 cut(s) 296
MlyI GAGTC 2 cut(s) 371, 821
MmeI TCCRAC 1 cut(s) 52
Mox20I TGGCCA 1 cut(s) 296
Mph1103I ATGCAT 1 cut(s) 287
MscI TGGCCA 1 cut(s) 296
MseI TTAA 4 cut(s) 288, 503, 789, 1006
MslI CAYNNNNRTG 3 cut(s) 630, 753, 836
Msp20I TGGCCA 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 228
MunI CAATTG 1 cut(s) 15
Mva1269I GAATGC 1 cut(s) 111
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 6 cut(s) 117, 489, 531, 601, 766, 902
NdeII GATC 5 cut(s) 306, 694, 778, 922, 946
NlaIII CATG 4 cut(s) 314, 779, 838, 869
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 1 cut(s) 157
NsiI ATGCAT 1 cut(s) 287
NspI RCATGY 1 cut(s) 869
PctI GAATGC 1 cut(s) 111
PkrI GCNGC 6 cut(s) 52, 594, 639, 768, 786, 871
PleI GAGTC 2 cut(s) 370, 820
PpsI GAGTC 2 cut(s) 370, 820
PshBI ATTAAT 1 cut(s) 288
Psp124BI GAGCTC 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 308
PstI CTGCAG 2 cut(s) 180, 786
PsuI RGATCY 1 cut(s) 306
RsaI GTAC 1 cut(s) 588
RsaNI GTAC 1 cut(s) 587
RseI CAYNNNNRTG 3 cut(s) 630, 753, 836
SacI GAGCTC 1 cut(s) 149
SaqAI TTAA 4 cut(s) 288, 503, 789, 1006
SatI GCNGC 6 cut(s) 51, 593, 638, 767, 785, 870
Sau3AI GATC 5 cut(s) 306, 694, 778, 922, 946
SchI GAGTC 2 cut(s) 371, 821
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 2 cut(s) 149, 775
SfaNI GCATC 2 cut(s) 753, 828
SfcI CTRYAG 2 cut(s) 176, 782
SmiMI CAYNNNNRTG 3 cut(s) 630, 753, 836
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
Sse9I AATT 7 cut(s) 15, 133, 273, 289, 402, 510, 795
SsiI CCGC 3 cut(s) 142, 525, 637
SspMI CTAG 1 cut(s) 162
SstI GAGCTC 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 2 cut(s) 297, 558
TaaI ACNGT 1 cut(s) 61
TaqI TCGA 2 cut(s) 337, 815
TasI AATT 7 cut(s) 15, 133, 273, 289, 402, 510, 795
TatI WGTACW 1 cut(s) 586
TauI GCSGC 1 cut(s) 640
Tru1I TTAA 4 cut(s) 288, 503, 789, 1006
Tru9I TTAA 4 cut(s) 288, 503, 789, 1006
TscAI CASTG 2 cut(s) 64, 85
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 5 cut(s) 50, 592, 766, 784, 869
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 1 cut(s) 876
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 2 cut(s) 64, 85
VneI GTGCAC 1 cut(s) 771
VspI ATTAAT 1 cut(s) 288
XapI RAATTY 2 cut(s) 402, 510
XceI RCATGY 1 cut(s) 869
XspI CTAG 1 cut(s) 162
Zsp2I ATGCAT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.