Rorug07G0257300
ERF Family

P-loop nucleoside triphosphate hydrolase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000615
Physical Location & Seq
Forward (+)
24114328 .. 24120447
6120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0257300.1

Sequence Viewer

Length: 3354 bp
ATGGCCTCAGAATCTCCAATCAATACATCAATCGGGTCACCTAGGCTTCGCCGTGTTAGCCAAACAAGCCATGATCTAAACAGTGACATTGGGGGTGTCGAATTCGCAACCTACACAGTCCACATACCCTCCACACCTGACAACAACCCCATGTCAGCTTCCATGGAGTACTCCACTTCTCAGAGAGTGGAGGACCAATTCGTCTCCAGCTCTCTCTTCACCGGCGGTTACAATTGCGTCACGCAAGCCCAGTTGAAGGAAAAGGTCATTGAATCACAGACAAGCTACCATCCCCAGATGACTGGGGCAAAAGGGTCTTTCTGCGCCGTGCCGGGTTGTGATGCTAAGGTGGTGACTGATGAGAGGGGACTGGATATTCTCCCTTGTGAATGTGACTACAAGATTTGTATGGATTGTTTTAGGGATGCTGTGAGAAATGGTGACCACATGTGTCCTGGCTGCAAGGAGCCTTATAAGGAGCTGGATGTGACCGAGTATGCAGTGCATAATCGAAAGCAGTATCAGCAGCAGATGTCGAAAACAGAGAGGAGGATGTCGTTGATGAAGTCGACGAAGGTGATGGGGCGCCAGAGTAGCGAGTTTGATCACAACAAGTGGTTGTTTGAGACAAAGGGGAGTTATGGATATGGAAATGCTATTTGGCCAAAGGTAGATGTGGAAGGGAGTCAAGATGGGATTGGGGGAGATCCGATGGTGTTTCATGATAAGCAATGGAGGCCTCTTACGCGAAAGTTGAATATCTCTGCTGCAATTCTCAGTCCATATAGGCTTTTAATATTTGTTCGAATGGTGGTCCTTGGATTGTTTTTGCAATGGAGAATCAGAAATCCAAATGAAGATGCTGTCTGGTTGTGGGCTATGTCAGTGGTTTGTGAAATCTGGTTTGCCATCTCATGGCTACTTGATCAGCTTCCAAAGCTCTGCCCCATCAATCGCATTACCAATCTCGATGTGTTAAAGGAGAAATTTGAAACACCGAGCCTCAGCAATCCTACTGGAAAATCTGATCTTCCAGGCATAGACATTTTTGTCTCTACAGCAGATCCAGAGAAAGAACCACCTCTTGTCACCGCAAACACAATTCTGTCGATTCTTGCAGCTGACTACCCTGTTGAGAAGCTTGCATGTTATGTTTCTGATGATGGAGGTGCACTTTTGACATTTGAGGCCATGGCCGAAGCTGCTAGTTTTGCCAACTTGTGGGTACCTTTTTGCCGGAAGCATAATATTGAGCCAAGAAACCCAGAATCTTACTTCAGTCTGAAGAGAGATCCCTACAAGAACAAAGTACGACAAGATTTTGTTAGAGACCGAAGGAGAGTAAAGCGTGAATATGATGAATATAAGGTTAGGATTAATGGCCTTCCTGATTCAATTCGGAGACGTTCAGAGGCCTACAATGCTAGGGACGAGATGAAGGCTATGAAACTTGGGAGAGCGATTGGAAATGACGAAGAAGTTGATAAGATAAAGATCCCAAAAGCAACTTGGATGGCTGATGGAACACACTGGCCTGGTACCTGGACAGTTCCTTCCCCTGAGCATTCTAGGGGTGATCATGCTAGTATCATGCAGGTGATGTTGAAACCTCCTAGTGATGAACCACTGAAAGGAACAACAGTGGATTCAAATTCCATGGATCTAAGTGAGGTTGACATTCGTCTTCCAATGTTAGTTTATGTTTCTCGTGAGAAGCGACCTGGCTATGATCACAACAAGAAGGCTGGTGCTATGAATGCCTTGGTTCGTGCCTCAGCTGTTATGTCCAATGGCCCCTTCATTCTCAACCTTGACTGTGACCACTACATCTACAATTCCCAAGCATTGAGAGAAGGCATGTGCTTCATGATGGATCGTGGTGGGGACCGCATTAGTTATGTCCAGTTTCCTCAAAGGTTTGAAGGAATAGACCCTTCTGACCGTTATGCCAACCACAACACTGTTTTCTTTGATGTCAACATGCGTGCACTTGATGGCATTCAGGGTCCAGTTTATGTTGGAACAGGTTGCCTCTTTCGACGAACTGCCCTTTATGGATTTGAGCCACCTCAAGTGAAAGATCGTAATGGTTGTTGCTGTGGTGGCCGCAATAAGAAATCTAAATCTTCTTCAGTTGTTTCTGCCCCTGAAATTGACTCTCAAACAATACAAGTGGGAGACTTTGATGATGATAACGAAATGAATGCCATTATTCCTAAAAGGTTTGGGAACTCAAGTTTCCTTGTAGACTCTATCCAAGTGGCAGAATTCCAAGGTCGTCCTCTTGCTGATCATCCATCATTGAAAAATGGACGACCGCCAGGTGCTCTCACATTTCCTCGTGAGCTTCTTGATGCATCAACAGTTGCAGAGGCAATCAGTGTCATCTCCTGCTGGTATGAGGATAAGACTGAATGGGGCCAACGCATTGGGTGGATTTATGGTTCAGTAACTGAAGATGTTGTCACAGGTTATAGGATGCATAATAGAGGATGGAGGTCTGTTTATTGTGTAACAAAAAGAGATGCATTTCGGGGTTCTGCCCCAATCAATCTCACTGATCGCCTTCACCAGGTTCTCCGATGGGCAACTGGATCTGTCGAGATTTTCTTTTCCCGAAACAATGCTCTTTTCGCAAGCTCCAAAATGAAGTTTCTACAAAGGATAGCATACCTCAATGTTGGAATGTACCCTTTCACTTCCTTTTTCCTCATTGTCTACTGCTTCCTTCCAGCACTTTCACTCATTTCCGGCCAGTTCATAGTTCAGACCCTCAACATAACCTTCCTGGTATACCTTTTAGGCATCACATTGACCCTCATTCTTCTTGCTGTGCTGGAGATCAAGTGGTCTGGAATTGAACTAGAAGAGTGGTGGAGGAATGAGCAGTTTTGGTTGATTGGAGGAACGAGCGCCCATCTTGCAGCTGTGCTTCAAGGTCTTCTAAAGGTCATAGCAGGTATTGAGATCTCATTCACACTGACATCAAAATCTGGAGGTGATGATATAGATGATGAGTTTGTGGATTTGTATATCTTCAAATGGACATCACTGATGATACCGCCAATCACAATCATGATGACAAACTTGATTGGAATAGCTATTGCTGTTTGTCGAACGGTATATAGTGTCATACCACAGTGGAGCAAATTGCTTGGAGGAGTGTTCTTCAGTTTTTGGGTGCTGGCGCATCTCTATCCCTTTGCAAAAGGGCTTATGGGAAGACGAGGGAAGACACCGACCATTGTGTTTGTGTGGTCTGGTCTTATGGCAATCACCATATCTCTCCTTTGGGTGGCTATAAGTCCCCCATCAGGTACTAATCAAATTGGTGGGTCATTCCAGTTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AIG1

Protein Analysis

1117

Amino Acids

125.75

Weight (kDa)

7.01

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_4 PF14570 110 - 159 4e-17 RING/Ubox like zinc-binding domain
Cellulose_synt PF03552 347 - 1108 0.0 Cellulose synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000615)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33970 AT1G33970 AT1G33970 AT1G33970 AT1G33970
fragaria_vesca FvH4_5g32530 FvH4_5g32530
malus_domestica MD15G1403700.v1.1
prunus_persica Prupe.1G549700_v2.0.a1 Prupe.1G549700_v2.0.a1
pyrus_communis pycom15g36100
rosa_chinensis RchiOBHm_Chr7g0226331 RchiOBHm_Chr7g0229101 RchiOBHm_Chr7g0229111 RchiOBHm_Chr7g0229141 RchiOBHm_Chr7g0229161 RchiOBHm_Chr7g0229171 RchiOBHm_Chr7g0229211 RchiOBHm_Chr7g0229241 RchiOBHm_Chr7g0229381 RchiOBHm_Chr7g0229761 RchiOBHm_Chr7g0229911
rosa_laevigata RLG00000000796 RLG00000001464 RLG00000001466 RLG00000001469 RLG00000001523 RLG00000001526 RLG00000001529 RLG00000001530 RLG00000001749 RLG00000001913 RLG00000002114 RLG00000002115 RLG00000023248 RLG00000023250
rosa_multiflora Rmu_co8281135.1_g000001 Rmu_sc0000271.1_g000002 Rmu_sc0000271.1_g000007 Rmu_sc0000271.1_g000014 Rmu_sc0000693.1_g000012 Rmu_sc0000693.1_g000015 Rmu_sc0000693.1_g000023 Rmu_sc0001044.1_g000005 Rmu_sc0006689.1_g000007 Rmu_sc0012733.1_g000005
rosa_roxburghii Rroxscaffold_3G00230540 Rroxscaffold_3G00230590 Rroxscaffold_3G00230600 Rroxscaffold_3G00230700 Rroxscaffold_3G00230710 Rroxscaffold_3G00232980 Rroxscaffold_3G00237610
rosa_rugosa Rorug07G0203800 Rorug07G0203900 Rorug07G0256100 Rorug07G0256400 Rorug07G0256400 Rorug07G0256400 Rorug07G0256500 Rorug07G0256600 Rorug07G0256600 Rorug07G0257100 Rorug07G0257200 Rorug07G0257300 Rorug07G0257400
rosa_samantha Rh7DG401100 Rh7DG401300 Rh7DG401900 Rh7DG402100 Rh7DG402300 Rh7DG402700
rosa_wichuraiana Rw0G001910 Rw0G001930 Rw0G001940 Rw7G033790 Rw7G033810 Rw7G033840 Rw7G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 474
AarI CACCTGC 1 cut(s) 1585
AasI GACNNNNNNGTC 2 cut(s) 200, 1049
Acc36I ACCTGC 2 cut(s) 1585, 2948
Acc65I GGTACC 2 cut(s) 1225, 1538
AccB1I GGYRCC 3 cut(s) 585, 1225, 1538
AccB7I CCANNNNNTGG 2 cut(s) 915, 1221
AccI GTMKAC 4 cut(s) 569, 2247, 2718, 2793
AccII CGCG 1 cut(s) 748
AciI CCGC 6 cut(s) 225, 1092, 1888, 2107, 2318, 3062
AclWI GGATC 7 cut(s) 701, 1058, 1286, 1489, 1668, 1881, 2602
AcoI YGGCCR 4 cut(s) 662, 1194, 2104, 2752
AcsI RAATTY 4 cut(s) 101, 986, 1651, 2267
AcuI CTGAAG 5 cut(s) 1261, 1304, 2115, 2475, 3154
AcyI GRCGYC 1 cut(s) 586
AfaI GTAC 6 cut(s) 170, 1227, 1311, 1540, 2690, 3319
AfiI CCNNNNNNNGG 7 cut(s) 256, 915, 1221, 1631, 2572, 3295, 3314
AflIII ACRYGT 1 cut(s) 447
AjnI CCWGG 8 cut(s) 454, 1033, 1534, 1541, 1720, 2320, 2571, 2787
AjuI GAANNNNNNNTTGG 4 cut(s) 643, 675, 1781, 1813
AloI GAACNNNNNNTCC 2 cut(s) 2428, 2460
Alw21I GWGCWC 3 cut(s) 1174, 1990, 2329
Alw26I GTCTC 6 cut(s) 208, 620, 1057, 1323, 1396, 2172
Alw44I GTGCAC 2 cut(s) 1170, 1986
AlwI GGATC 7 cut(s) 701, 1058, 1286, 1489, 1668, 1881, 2602
AlwNI CAGNNNCTG 1 cut(s) 2453
ApaLI GTGCAC 2 cut(s) 1170, 1986
ApeKI GCWGC 6 cut(s) 459, 526, 767, 1118, 1202, 2924
ApoI RAATTY 4 cut(s) 101, 986, 1651, 2267
AseI ATTAAT 1 cut(s) 1377
Asp718I GGTACC 2 cut(s) 1225, 1538
AspA2I CCTAGG 1 cut(s) 41
AspLEI GCGC 4 cut(s) 326, 588, 2915, 3190
AspS9I GGNCC 6 cut(s) 193, 814, 1793, 1885, 2006, 2419
AsuC2I CCSGG 1 cut(s) 333
AsuII TTCGAA 1 cut(s) 805
AvaII GGWCC 4 cut(s) 193, 814, 1885, 2006
AvrII CCTAGG 1 cut(s) 41
BaeGI GKGCMC 2 cut(s) 1174, 1990
BalI TGGCCA 1 cut(s) 664
BanI GGYRCC 3 cut(s) 585, 1225, 1538
BauI CACGAG 2 cut(s) 1707, 2340
BbsI GAAGAC 4 cut(s) 1676, 2933, 3229, 3239
Bbv12I GWGCWC 3 cut(s) 1174, 1990, 2329
BbvCI CCTCAGC 2 cut(s) 1004, 1774
BbvI GCAGC 6 cut(s) 446, 538, 754, 1130, 1189, 2936
BceAI ACGGC 2 cut(s) 36, 311
BcgI CGANNNNNNTGC 2 cut(s) 2186, 2220
BciT130I CCWGG 8 cut(s) 456, 1035, 1536, 1543, 1722, 2322, 2573, 2789
BclI TGATCA 5 cut(s) 604, 925, 1576, 1729, 2290
BcnI CCSGG 1 cut(s) 333
BcoDI GTCTC 6 cut(s) 208, 620, 1057, 1323, 1396, 2172
BfaI CTAG 7 cut(s) 42, 1206, 1425, 1569, 1584, 1614, 2864
BfmI CTRYAG 1 cut(s) 1056
BfoI RGCGCY 2 cut(s) 589, 2916
BfuAI ACCTGC 2 cut(s) 1585, 2948
BglII AGATCT 1 cut(s) 2967
BisI GCNGC 7 cut(s) 460, 527, 768, 1119, 1203, 2107, 2925
BlnI CCTAGG 1 cut(s) 41
BlsI GCNGC 7 cut(s) 461, 528, 769, 1120, 1204, 2108, 2926
BmcAI AGTACT 1 cut(s) 170
Bme1390I CCNGG 9 cut(s) 333, 456, 1035, 1536, 1543, 1722, 2322, 2573, 2789
Bme18I GGWCC 4 cut(s) 193, 814, 1885, 2006
BmgT120I GGNCC 6 cut(s) 193, 814, 1793, 1885, 2006, 2419
BmiI GGNNCC 8 cut(s) 468, 587, 1227, 1540, 1795, 1886, 2007, 2420
BmrFI CCNGG 9 cut(s) 333, 456, 1035, 1536, 1543, 1722, 2322, 2573, 2789
BmrI ACTGGG 2 cut(s) 244, 312
BmsI GCATC 9 cut(s) 331, 415, 850, 2344, 2366, 2469, 2515, 2814, 3199
BmuI ACTGGG 2 cut(s) 244, 312
BoxI GACNNNNGTC 1 cut(s) 1680
BpiI GAAGAC 4 cut(s) 1676, 2933, 3229, 3239
BpmI CTGGAG 3 cut(s) 190, 2858, 3015
Bpu10I CCTNAGC 4 cut(s) 345, 1004, 1560, 1774
Bpu14I TTCGAA 1 cut(s) 805
BpuEI CTTGAG 2 cut(s) 2055, 2218
BpuMI CCSGG 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 1493
BsaHI GRCGYC 1 cut(s) 586
BsaI GGTCTC 1 cut(s) 1323
BsaJI CCNNGG 7 cut(s) 41, 162, 817, 1191, 1656, 1761, 2272
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 2831, 2861
Bsc4I CCNNNNNNNGG 7 cut(s) 256, 915, 1221, 1631, 2572, 3295, 3314
Bse118I RCCGGY 1 cut(s) 221
Bse3DI GCAATG 2 cut(s) 737, 839
Bse8I GATNNNNATC 1 cut(s) 1493
BseBI CCWGG 8 cut(s) 456, 1035, 1536, 1543, 1722, 2322, 2573, 2789
BseDI CCNNGG 7 cut(s) 41, 162, 817, 1191, 1656, 1761, 2272
BseGI GGATG 8 cut(s) 289, 430, 490, 558, 1518, 2293, 2484, 2498
BseJI GATNNNNATC 1 cut(s) 1493
BseLI CCNNNNNNNGG 7 cut(s) 256, 915, 1221, 1631, 2572, 3295, 3314
BseMI GCAATG 2 cut(s) 737, 839
BseMII CTCAG 6 cut(s) 21, 194, 790, 1018, 1551, 1788
BseRI GAGGAG 2 cut(s) 562, 3174
BseSI GKGCMC 2 cut(s) 1174, 1990
BseXI GCAGC 6 cut(s) 446, 538, 754, 1130, 1189, 2936
Bsh1236I CGCG 1 cut(s) 748
Bsh1285I CGRYCG 1 cut(s) 2318
BshNI GGYRCC 3 cut(s) 585, 1225, 1538
BsiEI CGRYCG 1 cut(s) 2318
BsiHKAI GWGCWC 3 cut(s) 1174, 1990, 2329
BsiSI CCGG 4 cut(s) 222, 332, 1237, 2751
BslFI GGGAC 4 cut(s) 381, 1442, 1898, 3291
BslI CCNNNNNNNGG 7 cut(s) 256, 915, 1221, 1631, 2572, 3295, 3314
BsmAI GTCTC 6 cut(s) 208, 620, 1057, 1323, 1396, 2172
BsmBI CGTCTC 2 cut(s) 208, 1396
BsmFI GGGAC 4 cut(s) 381, 1442, 1898, 3291
BsmI GAATGC 4 cut(s) 1564, 1762, 1998, 2209
Bso31I GGTCTC 1 cut(s) 1323
Bsp119I TTCGAA 1 cut(s) 805
Bsp1286I GDGCHC 3 cut(s) 1174, 1990, 2329
Bsp19I CCATGG 3 cut(s) 162, 1191, 1656
BspACI CCGC 6 cut(s) 225, 1092, 1888, 2107, 2318, 3062
BspCNI CTCAG 6 cut(s) 20, 193, 789, 1017, 1552, 1787
BspFNI CGCG 1 cut(s) 748
BspHI TCATGA 3 cut(s) 721, 1866, 3075
BspLI GGNNCC 8 cut(s) 468, 587, 1227, 1540, 1795, 1886, 2007, 2420
BspMI ACCTGC 2 cut(s) 1585, 2948
BspPI GGATC 7 cut(s) 701, 1058, 1286, 1489, 1668, 1881, 2602
BspT104I TTCGAA 1 cut(s) 805
BspT107I GGYRCC 3 cut(s) 585, 1225, 1538
BspTNI GGTCTC 1 cut(s) 1323
BsrDI GCAATG 2 cut(s) 737, 839
BsrFI RCCGGY 1 cut(s) 221
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 7 cut(s) 41, 162, 817, 1191, 1656, 1761, 2272
BssNAI GTATAC 1 cut(s) 2794
BssNI GRCGYC 1 cut(s) 586
BssSI CACGAG 2 cut(s) 1707, 2340
BssT1I CCWWGG 7 cut(s) 41, 162, 817, 1191, 1656, 1761, 2272
Bst1107I GTATAC 1 cut(s) 2794
Bst2BI CACGAG 2 cut(s) 1707, 2340
Bst2UI CCWGG 8 cut(s) 456, 1035, 1536, 1543, 1722, 2322, 2573, 2789
Bst6I CTCTTC 3 cut(s) 221, 1280, 2862
BstACI GRCGYC 1 cut(s) 586
BstBI TTCGAA 1 cut(s) 805
BstC8I GCNNGC 5 cut(s) 246, 1143, 1986, 2638, 3186
BstDEI CTNAG 8 cut(s) 7, 180, 345, 776, 1004, 1560, 1664, 1774
BstDSI CCRYGG 3 cut(s) 162, 1191, 1656
BstEII GGTNACC 2 cut(s) 36, 440
BstENI CCTNNNNNAGG 1 cut(s) 2570
BstF5I GGATG 8 cut(s) 289, 430, 490, 558, 1518, 2293, 2484, 2498
BstFNI CGCG 1 cut(s) 748
BstH2I RGCGCY 2 cut(s) 589, 2916
BstHHI GCGC 4 cut(s) 326, 588, 2915, 3190
BstMAI GTCTC 6 cut(s) 208, 620, 1057, 1323, 1396, 2172
BstMCI CGRYCG 1 cut(s) 2318
BstNI CCWGG 8 cut(s) 456, 1035, 1536, 1543, 1722, 2322, 2573, 2789
BstNSI RCATGY 4 cut(s) 451, 1149, 1861, 1984
BstPAI GACNNNNGTC 1 cut(s) 1680
BstPI GGTNACC 2 cut(s) 36, 440
BstSCI CCNGG 9 cut(s) 331, 454, 1033, 1534, 1541, 1720, 2320, 2571, 2787
BstSFI CTRYAG 1 cut(s) 1056
BstSLI GKGCMC 2 cut(s) 1174, 1990
BstUI CGCG 1 cut(s) 748
BstV1I GCAGC 6 cut(s) 446, 538, 754, 1130, 1189, 2936
BstV2I GAAGAC 4 cut(s) 1676, 2933, 3229, 3239
BstX2I RGATCY 7 cut(s) 706, 1063, 1291, 1494, 1660, 2594, 2967
BstXI CCANNNNNNTGG 2 cut(s) 302, 2429
BstYI RGATCY 7 cut(s) 706, 1063, 1291, 1494, 1660, 2594, 2967
BstZ17I GTATAC 1 cut(s) 2794
BtgI CCRYGG 3 cut(s) 162, 1191, 1656
BtsCI GGATG 8 cut(s) 289, 430, 490, 558, 1518, 2293, 2484, 2498
BtsI GCAGTG 1 cut(s) 507
BveI ACCTGC 2 cut(s) 1585, 2948
Cac8I GCNNGC 5 cut(s) 246, 1143, 1986, 2638, 3186
CaiI CAGNNNCTG 1 cut(s) 2453
CciI TCATGA 3 cut(s) 721, 1866, 3075
CfoI GCGC 4 cut(s) 326, 588, 2915, 3190
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 6 cut(s) 193, 814, 1793, 1885, 2006, 2419
CseI GACGC 1 cut(s) 226
CsiI ACCWGGT 1 cut(s) 2571
Csp6I GTAC 6 cut(s) 169, 1226, 1310, 1539, 2689, 3318
CspCI CAANNNNNGTGG 2 cut(s) 2154, 2189
CviQI GTAC 6 cut(s) 169, 1226, 1310, 1539, 2689, 3318
DdeI CTNAG 8 cut(s) 7, 180, 345, 776, 1004, 1560, 1664, 1774
DinI GGCGCC 1 cut(s) 587
DrdI GACNNNNNNGTC 2 cut(s) 200, 1049
DseDI GACNNNNNNGTC 2 cut(s) 200, 1049
EaeI YGGCCR 4 cut(s) 662, 1194, 2104, 2752
Eam1104I CTCTTC 3 cut(s) 221, 1280, 2862
EarI CTCTTC 3 cut(s) 221, 1280, 2862
Eco130I CCWWGG 7 cut(s) 41, 162, 817, 1191, 1656, 1761, 2272
Eco147I AGGCCT 2 cut(s) 739, 1415
Eco31I GGTCTC 1 cut(s) 1323
Eco47I GGWCC 4 cut(s) 193, 814, 1885, 2006
Eco57I CTGAAG 5 cut(s) 1261, 1304, 2115, 2475, 3154
Eco91I GGTNACC 2 cut(s) 36, 440
EcoNI CCTNNNNNAGG 1 cut(s) 2570
EcoO65I GGTNACC 2 cut(s) 36, 440
EcoRI GAATTC 2 cut(s) 101, 2267
EcoRII CCWGG 8 cut(s) 454, 1033, 1534, 1541, 1720, 2320, 2571, 2787
EcoT14I CCWWGG 7 cut(s) 41, 162, 817, 1191, 1656, 1761, 2272
EcoT22I ATGCAT 3 cut(s) 2359, 2484, 2530
EgeI GGCGCC 1 cut(s) 587
EheI GGCGCC 1 cut(s) 587
ErhI CCWWGG 7 cut(s) 41, 162, 817, 1191, 1656, 1761, 2272
Esp3I CGTCTC 2 cut(s) 208, 1396
FaqI GGGAC 4 cut(s) 381, 1442, 1898, 3291
FbaI TGATCA 5 cut(s) 604, 925, 1576, 1729, 2290
FblI GTMKAC 4 cut(s) 569, 2247, 2718, 2793
Fnu4HI GCNGC 7 cut(s) 460, 527, 768, 1119, 1203, 2107, 2925
FokI GGATG 8 cut(s) 276, 437, 497, 565, 1525, 2280, 2491, 2505
Fsp4HI GCNGC 7 cut(s) 460, 527, 768, 1119, 1203, 2107, 2925
FspBI CTAG 7 cut(s) 42, 1206, 1425, 1569, 1584, 1614, 2864
GlaI GCGC 4 cut(s) 325, 587, 2914, 3189
GluI GCNGC 7 cut(s) 460, 527, 768, 1119, 1203, 2107, 2925
GsuI CTGGAG 3 cut(s) 190, 2858, 3015
HaeII RGCGCY 2 cut(s) 589, 2916
HapII CCGG 4 cut(s) 222, 332, 1237, 2751
HgaI GACGC 1 cut(s) 226
HhaI GCGC 4 cut(s) 326, 588, 2915, 3190
Hin1I GRCGYC 1 cut(s) 586
Hin6I GCGC 4 cut(s) 324, 586, 2913, 3188
HinP1I GCGC 4 cut(s) 324, 586, 2913, 3188
HincII GTYRAC 3 cut(s) 570, 1675, 1978
HindII GTYRAC 3 cut(s) 570, 1675, 1978
HindIII AAGCTT 1 cut(s) 1139
HpaII CCGG 4 cut(s) 222, 332, 1237, 2751
Hpy166II GTNNAC 9 cut(s) 121, 570, 1172, 1675, 1978, 1988, 2248, 2719, 2794
Hpy8I GTNNAC 9 cut(s) 121, 570, 1172, 1675, 1978, 1988, 2248, 2719, 2794
Hpy99I CGWCG 2 cut(s) 574, 2043
HpyCH4IV ACGT 1 cut(s) 1405
HpyF3I CTNAG 8 cut(s) 7, 180, 345, 776, 1004, 1560, 1664, 1774
HpySE526I ACGT 1 cut(s) 1405
Hsp92I GRCGYC 1 cut(s) 586
HspAI GCGC 4 cut(s) 324, 586, 2913, 3188
KasI GGCGCC 1 cut(s) 585
KpnI GGTACC 2 cut(s) 1229, 1542
Ksp22I TGATCA 5 cut(s) 604, 925, 1576, 1729, 2290
LmnI GCTCC 4 cut(s) 466, 478, 2645, 3144
Lsp1109I GCAGC 6 cut(s) 446, 538, 754, 1130, 1189, 2936
LweI GCATC 9 cut(s) 331, 415, 850, 2344, 2366, 2469, 2515, 2814, 3199
MabI ACCWGGT 1 cut(s) 2571
MaeI CTAG 7 cut(s) 42, 1206, 1425, 1569, 1584, 1614, 2864
MaeII ACGT 1 cut(s) 1405
MfeI CAATTG 1 cut(s) 232
MflI RGATCY 7 cut(s) 706, 1063, 1291, 1494, 1660, 2594, 2967
MhlI GDGCHC 3 cut(s) 1174, 1990, 2329
MlsI TGGCCA 1 cut(s) 664
MluNI TGGCCA 1 cut(s) 664
Mly113I GGCGCC 1 cut(s) 586
MlyI GAGTC 3 cut(s) 694, 2150, 2243
MmeI TCCRAC 2 cut(s) 1999, 2662
Mox20I TGGCCA 1 cut(s) 664
Mph1103I ATGCAT 3 cut(s) 2359, 2484, 2530
MscI TGGCCA 1 cut(s) 664
MseI TTAA 3 cut(s) 794, 977, 1377
MslI CAYNNNNRTG 2 cut(s) 1595, 3074
Msp20I TGGCCA 1 cut(s) 664
MspA1I CMGCKG 3 cut(s) 1121, 1778, 2927
MspI CCGG 4 cut(s) 222, 332, 1237, 2751
MspR9I CCNGG 9 cut(s) 333, 456, 1035, 1536, 1543, 1722, 2322, 2573, 2789
MunI CAATTG 1 cut(s) 232
Mva1269I GAATGC 4 cut(s) 1564, 1762, 1998, 2209
MvaI CCWGG 8 cut(s) 456, 1035, 1536, 1543, 1722, 2322, 2573, 2789
MvnI CGCG 1 cut(s) 748
NarI GGCGCC 1 cut(s) 586
NciI CCSGG 1 cut(s) 333
NcoI CCATGG 3 cut(s) 162, 1191, 1656
NlaIV GGNNCC 8 cut(s) 468, 587, 1227, 1540, 1795, 1886, 2007, 2420
NsiI ATGCAT 3 cut(s) 2359, 2484, 2530
NspI RCATGY 4 cut(s) 451, 1149, 1861, 1984
NspV TTCGAA 1 cut(s) 805
PagI TCATGA 3 cut(s) 721, 1866, 3075
PaqCI CACCTGC 1 cut(s) 1585
PceI AGGCCT 2 cut(s) 739, 1415
PciI ACATGT 1 cut(s) 447
PcsI WCGNNNNNNNCGW 1 cut(s) 1714
PctI GAATGC 4 cut(s) 1564, 1762, 1998, 2209
PfeI GAWTC 7 cut(s) 11, 272, 840, 1111, 1268, 1391, 1646
PflMI CCANNNNNTGG 2 cut(s) 915, 1221
PkrI GCNGC 7 cut(s) 461, 528, 769, 1120, 1204, 2108, 2926
PleI GAGTC 3 cut(s) 693, 2150, 2243
PluTI GGCGCC 1 cut(s) 589
PpsI GAGTC 3 cut(s) 693, 2150, 2243
PscI ACATGT 1 cut(s) 447
PshAI GACNNNNGTC 1 cut(s) 1680
PshBI ATTAAT 1 cut(s) 1377
PsiI TTATAA 1 cut(s) 474
Psp6I CCWGG 8 cut(s) 454, 1033, 1534, 1541, 1720, 2320, 2571, 2787
PspEI GGTNACC 2 cut(s) 36, 440
PspGI CCWGG 8 cut(s) 454, 1033, 1534, 1541, 1720, 2320, 2571, 2787
PspN4I GGNNCC 8 cut(s) 468, 587, 1227, 1540, 1795, 1886, 2007, 2420
PspPI GGNCC 6 cut(s) 193, 814, 1793, 1885, 2006, 2419
PstNI CAGNNNCTG 1 cut(s) 2453
PsuI RGATCY 7 cut(s) 706, 1063, 1291, 1494, 1660, 2594, 2967
PvuII CAGCTG 3 cut(s) 1121, 1778, 2927
RsaI GTAC 6 cut(s) 170, 1227, 1311, 1540, 2690, 3319
RsaNI GTAC 6 cut(s) 169, 1226, 1310, 1539, 2689, 3318
RseI CAYNNNNRTG 2 cut(s) 1595, 3074
SalI GTCGAC 1 cut(s) 568
SaqAI TTAA 3 cut(s) 794, 977, 1377
SatI GCNGC 7 cut(s) 460, 527, 768, 1119, 1203, 2107, 2925
Sau96I GGNCC 6 cut(s) 193, 814, 1793, 1885, 2006, 2419
ScaI AGTACT 1 cut(s) 170
SchI GAGTC 3 cut(s) 694, 2150, 2243
ScrFI CCNGG 9 cut(s) 333, 456, 1035, 1536, 1543, 1722, 2322, 2573, 2789
SduI GDGCHC 3 cut(s) 1174, 1990, 2329
SexAI ACCWGGT 1 cut(s) 2571
SfaNI GCATC 9 cut(s) 331, 415, 850, 2344, 2366, 2469, 2515, 2814, 3199
SfcI CTRYAG 1 cut(s) 1056
SfoI GGCGCC 1 cut(s) 587
SfuI TTCGAA 1 cut(s) 805
SgrAI CRCCGGYG 1 cut(s) 221
SinI GGWCC 4 cut(s) 193, 814, 1885, 2006
SmiMI CAYNNNNRTG 2 cut(s) 1595, 3074
SmlI CTYRAG 2 cut(s) 2070, 2233
SmoI CTYRAG 2 cut(s) 2070, 2233
SseBI AGGCCT 2 cut(s) 739, 1415
SsiI CCGC 6 cut(s) 225, 1092, 1888, 2107, 2318, 3062
SspDI GGCGCC 1 cut(s) 585
SspI AATATT 2 cut(s) 798, 1249
SspMI CTAG 7 cut(s) 42, 1206, 1425, 1569, 1584, 1614, 2864
StuI AGGCCT 2 cut(s) 739, 1415
StyD4I CCNGG 9 cut(s) 331, 454, 1033, 1534, 1541, 1720, 2320, 2571, 2787
StyI CCWWGG 7 cut(s) 41, 162, 817, 1191, 1656, 1761, 2272
TaiI ACGT 1 cut(s) 1408
TaqII GACCGA 2 cut(s) 506, 1347
TatI WGTACW 1 cut(s) 168
TauI GCSGC 1 cut(s) 2109
TfiI GAWTC 7 cut(s) 11, 272, 840, 1111, 1268, 1391, 1646
Tru1I TTAA 3 cut(s) 794, 977, 1377
Tru9I TTAA 3 cut(s) 794, 977, 1377
TseI GCWGC 6 cut(s) 459, 526, 767, 1118, 1202, 2924
Van91I CCANNNNNTGG 2 cut(s) 915, 1221
VneI GTGCAC 2 cut(s) 1170, 1986
VpaK11BI GGWCC 4 cut(s) 193, 814, 1885, 2006
VspI ATTAAT 1 cut(s) 1377
XagI CCTNNNNNAGG 1 cut(s) 2570
XapI RAATTY 4 cut(s) 101, 986, 1651, 2267
XceI RCATGY 4 cut(s) 451, 1149, 1861, 1984
XcmI CCANNNNNNNNNTGG 2 cut(s) 452, 1506
XmaJI CCTAGG 1 cut(s) 41
XmiI GTMKAC 4 cut(s) 569, 2247, 2718, 2793
XspI CTAG 7 cut(s) 42, 1206, 1425, 1569, 1584, 1614, 2864
ZrmI AGTACT 1 cut(s) 170
Zsp2I ATGCAT 3 cut(s) 2359, 2484, 2530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.