Rroxscaffold_3G00230540
ERF Family

P-loop nucleoside triphosphate hydrolase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000615
Physical Location & Seq
Reverse (-)
15424950 .. 15430627
5678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00230540.1

Sequence Viewer

Length: 1146 bp
ATGGATGAAAGTTCGATTGATGTTGTCAGGGAACATCCCGTTTCTAATGCTGCTCGCACTGTGGTCTTAGTTGGACGCACTGGTAATGGAAAAAGTGCAACAGGCAACAGCATCCTTGGCGAAAGAGCCTTCATTTCCAAGCGTAGCTCTAGGGGTGTCACCACCACTACTGAATTGAAGACTGCTATCTTGAGAGATGGACAACAAATTAACGTTGTAGACACTCCTGGTCTTTTTGATAGTTCTGTCAAATCAGACGTTATTAGCAAAGAAATTGTCCAATGCATTAAATTGGCCAAGGATGGAATCCATGCTATTCTTGTGATTCTCTCAACTAGAACTCGGTTTTCACAAGAAGAGCAATCTGCAATCCTTGGCTTGCAAGCTCTATTTGGAAGTAAAATATTTGACTATATGATTGTTGTCTTTTCGGGAGGAGATGAGCTTGAAGAAAATGATGAGACATTGGAAGATTATTTGGGCGGCCGTGATTGCCCGGAGCCTCTGAAGGAAATCCTTGGTCTGTGTGGAAATCGTTGTGTTCTTTTTGATAACAAGACCAAGGATGAAAGCAAGAGGGTCGAACAAGTTCAGCGGCTTCTCTCGCTTGTAAACTTGGTTATAGCACAGAATGGTGGGCAGCCATACACGGATGAGATATTTGCTGAAGTGAAGAAAGAGGCTATGAAACTTCGTGATCAACAAGAAGATGTTGCTTCAAGGGGGTATTCAAAACAAGAAATATCGCATTTGAATGAGCAGATGCAGCGTGCACATGATCTGCAGCTTAAACGAATTACGGAGATGATTGAGTCAAACATGAGAGAGAAAACCATGATGCTTGAACAAAAGTTAGCAGATGAACATGCTGCACGACTAAGAGCAGAAGAGACTGCCCAAAAGGCTCAACAAAGTTCTCAGGATGAAATCCGAAAGCTTAGAGGTGATCTAGAAGCAGAGAGGCAGCGGCCAAAACCGAGACCGCCCAGTTCAGAGTCAGCTGTTGCATCTTGGACCGACAGAGAGGTCTACATGAGGCGATATGTTGGTTCAAGTACCGTCATAAAATTGTTTAGAGGTTTTGCATCCAACATGATGCGGCATTGGAAGGGTCTATTGGAGACAGTTATAAGTGCTGATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AIG1

Protein Analysis

381

Amino Acids

42.79

Weight (kDa)

6.01

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RsgA_GTPase PF03193 18 - 78 1.3e-06 RsgA GTPase
AIG1 PF04548 20 - 235 4.2e-78 AIG1 family
MMR_HSR1 PF01926 20 - 120 4.8e-10 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000615)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33970 AT1G33970 AT1G33970 AT1G33970 AT1G33970
fragaria_vesca FvH4_5g32530 FvH4_5g32530
malus_domestica MD15G1403700.v1.1
prunus_persica Prupe.1G549700_v2.0.a1 Prupe.1G549700_v2.0.a1
pyrus_communis pycom15g36100
rosa_chinensis RchiOBHm_Chr7g0226331 RchiOBHm_Chr7g0229101 RchiOBHm_Chr7g0229111 RchiOBHm_Chr7g0229141 RchiOBHm_Chr7g0229161 RchiOBHm_Chr7g0229171 RchiOBHm_Chr7g0229211 RchiOBHm_Chr7g0229241 RchiOBHm_Chr7g0229381 RchiOBHm_Chr7g0229761 RchiOBHm_Chr7g0229911
rosa_laevigata RLG00000000796 RLG00000001464 RLG00000001466 RLG00000001469 RLG00000001523 RLG00000001526 RLG00000001529 RLG00000001530 RLG00000001749 RLG00000001913 RLG00000002114 RLG00000002115 RLG00000023248 RLG00000023250
rosa_multiflora Rmu_co8281135.1_g000001 Rmu_sc0000271.1_g000002 Rmu_sc0000271.1_g000007 Rmu_sc0000271.1_g000014 Rmu_sc0000693.1_g000012 Rmu_sc0000693.1_g000015 Rmu_sc0000693.1_g000023 Rmu_sc0001044.1_g000005 Rmu_sc0006689.1_g000007 Rmu_sc0012733.1_g000005
rosa_roxburghii Rroxscaffold_3G00230540 Rroxscaffold_3G00230590 Rroxscaffold_3G00230600 Rroxscaffold_3G00230700 Rroxscaffold_3G00230710 Rroxscaffold_3G00232980 Rroxscaffold_3G00237610
rosa_rugosa Rorug07G0203800 Rorug07G0203900 Rorug07G0256100 Rorug07G0256400 Rorug07G0256400 Rorug07G0256400 Rorug07G0256500 Rorug07G0256600 Rorug07G0256600 Rorug07G0257100 Rorug07G0257200 Rorug07G0257300 Rorug07G0257400
rosa_samantha Rh7DG401100 Rh7DG401300 Rh7DG401900 Rh7DG402100 Rh7DG402300 Rh7DG402700
rosa_wichuraiana Rw0G001910 Rw0G001930 Rw0G001940 Rw7G033790 Rw7G033810 Rw7G033840 Rw7G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1130
AasI GACNNNNNNGTC 1 cut(s) 1025
AccI GTMKAC 2 cut(s) 219, 1029
AciI CCGC 5 cut(s) 483, 595, 967, 983, 1099
AclI AACGTT 1 cut(s) 213
AcoI YGGCCR 3 cut(s) 294, 484, 968
AcuI CTGAAG 2 cut(s) 527, 687
AfaI GTAC 1 cut(s) 1057
AgsI TTSAA 7 cut(s) 178, 449, 720, 732, 754, 845, 1053
AjnI CCWGG 1 cut(s) 226
AjuI GAANNNNNNNTTGG 2 cut(s) 1100, 1132
AluBI AGCT 6 cut(s) 147, 386, 445, 787, 937, 1001
AluI AGCT 6 cut(s) 147, 386, 445, 787, 937, 1001
Alw21I GWGCWC 1 cut(s) 775
Alw26I GTCTC 4 cut(s) 455, 884, 973, 1115
Alw44I GTGCAC 1 cut(s) 771
AoxI GGCC 3 cut(s) 294, 484, 968
ApaLI GTGCAC 1 cut(s) 771
ApeKI GCWGC 6 cut(s) 50, 640, 766, 784, 869, 964
ArsI GACNNNNNNTTYG 2 cut(s) 261, 293
Asp700I GAANNNNTTC 1 cut(s) 588
AspS9I GGNCC 1 cut(s) 1014
AsuC2I CCSGG 1 cut(s) 497
AsuHPI GGTGA 2 cut(s) 151, 956
AvaII GGWCC 1 cut(s) 1014
BaeGI GKGCMC 1 cut(s) 775
BalI TGGCCA 1 cut(s) 296
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 775
BbvI GCAGC 6 cut(s) 37, 652, 778, 796, 856, 976
BccI CCATC 2 cut(s) 191, 296
BceAI ACGGC 1 cut(s) 471
BcgI CGANNNNNNTGC 2 cut(s) 562, 596
BciT130I CCWGG 1 cut(s) 228
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 1 cut(s) 497
BcoDI GTCTC 4 cut(s) 455, 884, 973, 1115
BfaI CTAG 3 cut(s) 150, 336, 950
BfmI CTRYAG 1 cut(s) 782
BglI GCCNNNNNGGC 1 cut(s) 902
Bme1390I CCNGG 2 cut(s) 228, 497
Bme18I GGWCC 1 cut(s) 1014
BmgT120I GGNCC 1 cut(s) 1014
BmiI GGNNCC 1 cut(s) 501
BmrFI CCNGG 2 cut(s) 228, 497
BmrI ACTGGG 1 cut(s) 981
BmsI GCATC 6 cut(s) 120, 753, 828, 1016, 1086, 1094
BmuI ACTGGG 1 cut(s) 981
BpiI GAAGAC 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 211
BpuMI CCSGG 1 cut(s) 497
BsaI GGTCTC 1 cut(s) 973
BsaJI CCNNGG 5 cut(s) 115, 297, 373, 517, 561
Bse1I ACTGG 2 cut(s) 85, 987
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 5 cut(s) 115, 297, 373, 517, 561
BseGI GGATG 8 cut(s) 10, 34, 111, 307, 571, 658, 928, 1085
BseMII CTCAG 1 cut(s) 932
BseNI ACTGG 2 cut(s) 85, 987
BseRI GAGGAG 1 cut(s) 450
BseSI GKGCMC 1 cut(s) 775
BseX3I CGGCCG 1 cut(s) 484
BseXI GCAGC 6 cut(s) 37, 652, 778, 796, 856, 976
BsgI GTGCAG 1 cut(s) 855
Bsh1285I CGRYCG 1 cut(s) 487
BshFI GGCC 3 cut(s) 296, 486, 970
BsiEI CGRYCG 1 cut(s) 487
BsiHKAI GWGCWC 1 cut(s) 775
BsiSI CCGG 1 cut(s) 497
BsmAI GTCTC 4 cut(s) 455, 884, 973, 1115
BsnI GGCC 3 cut(s) 296, 486, 970
Bso31I GGTCTC 1 cut(s) 973
Bsp1286I GDGCHC 1 cut(s) 775
Bsp143I GATC 3 cut(s) 697, 778, 946
BspACI CCGC 5 cut(s) 483, 595, 967, 983, 1099
BspANI GGCC 3 cut(s) 296, 486, 970
BspCNI CTCAG 1 cut(s) 931
BspLI GGNNCC 1 cut(s) 501
BspMAI CTGCAG 1 cut(s) 786
BspQI GCTCTTC 1 cut(s) 351
BspTNI GGTCTC 1 cut(s) 973
BsrI ACTGG 2 cut(s) 85, 987
BssECI CCNNGG 5 cut(s) 115, 297, 373, 517, 561
BssMI GATC 3 cut(s) 697, 778, 946
BssT1I CCWWGG 5 cut(s) 115, 297, 373, 517, 561
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 3 cut(s) 61, 1060, 1126
Bst6I CTCTTC 2 cut(s) 351, 882
BstC8I GCNNGC 4 cut(s) 55, 380, 384, 771
BstDEI CTNAG 4 cut(s) 67, 878, 918, 938
BstF5I GGATG 8 cut(s) 10, 34, 111, 307, 571, 658, 928, 1085
BstKTI GATC 3 cut(s) 700, 781, 949
BstMAI GTCTC 4 cut(s) 455, 884, 973, 1115
BstMBI GATC 3 cut(s) 697, 778, 946
BstMCI CGRYCG 1 cut(s) 487
BstMWI GCNNNNNNNGC 5 cut(s) 117, 492, 604, 766, 902
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 869
BstSCI CCNGG 2 cut(s) 226, 495
BstSFI CTRYAG 1 cut(s) 782
BstSLI GKGCMC 1 cut(s) 775
BstV1I GCAGC 6 cut(s) 37, 652, 778, 796, 856, 976
BstV2I GAAGAC 1 cut(s) 185
BstZI CGGCCG 1 cut(s) 484
BsuRI GGCC 3 cut(s) 296, 486, 970
BtsCI GGATG 8 cut(s) 10, 34, 111, 307, 571, 658, 928, 1085
BtsIMutI CAGTG 2 cut(s) 57, 78
Cac8I GCNNGC 4 cut(s) 55, 380, 384, 771
Cfr13I GGNCC 1 cut(s) 1014
CseI GACGC 1 cut(s) 84
Csp6I GTAC 1 cut(s) 1056
CviAII CATG 7 cut(s) 311, 776, 820, 835, 866, 1033, 1093
CviQI GTAC 1 cut(s) 1056
DdeI CTNAG 4 cut(s) 67, 878, 918, 938
DpnI GATC 3 cut(s) 699, 780, 948
DpnII GATC 3 cut(s) 697, 778, 946
DrdI GACNNNNNNGTC 1 cut(s) 1025
DseDI GACNNNNNNGTC 1 cut(s) 1025
EaeI YGGCCR 3 cut(s) 294, 484, 968
EagI CGGCCG 1 cut(s) 484
Eam1104I CTCTTC 2 cut(s) 351, 882
EarI CTCTTC 2 cut(s) 351, 882
EclXI CGGCCG 1 cut(s) 484
Eco130I CCWWGG 5 cut(s) 115, 297, 373, 517, 561
Eco31I GGTCTC 1 cut(s) 973
Eco47I GGWCC 1 cut(s) 1014
Eco52I CGGCCG 1 cut(s) 484
Eco57I CTGAAG 2 cut(s) 527, 687
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 5 cut(s) 115, 297, 373, 517, 561
EcoT22I ATGCAT 1 cut(s) 287
ErhI CCWWGG 5 cut(s) 115, 297, 373, 517, 561
FaeI CATG 7 cut(s) 314, 779, 823, 838, 869, 1036, 1096
FatI CATG 7 cut(s) 310, 775, 819, 834, 865, 1032, 1092
FbaI TGATCA 1 cut(s) 697
FblI GTMKAC 2 cut(s) 219, 1029
FokI GGATG 8 cut(s) 17, 21, 98, 314, 578, 665, 935, 1072
FspBI CTAG 3 cut(s) 150, 336, 950
HaeIII GGCC 3 cut(s) 296, 486, 970
HapII CCGG 1 cut(s) 497
HgaI GACGC 1 cut(s) 84
Hin1II CATG 7 cut(s) 314, 779, 823, 838, 869, 1036, 1096
HindIII AAGCTT 1 cut(s) 935
HinfI GANTC 4 cut(s) 306, 325, 812, 995
HpaII CCGG 1 cut(s) 497
HphI GGTGA 2 cut(s) 151, 956
Hpy166II GTNNAC 4 cut(s) 220, 613, 773, 1030
Hpy188I TCNGA 4 cut(s) 256, 507, 932, 994
Hpy188III TCNNGA 5 cut(s) 190, 432, 695, 920, 950
Hpy8I GTNNAC 4 cut(s) 220, 613, 773, 1030
HpyAV CCTTC 3 cut(s) 139, 502, 1102
HpyCH4III ACNGT 3 cut(s) 61, 1060, 1126
HpyCH4IV ACGT 2 cut(s) 213, 258
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 492, 604, 766, 902
HpyF3I CTNAG 4 cut(s) 67, 878, 918, 938
HpySE526I ACGT 2 cut(s) 213, 258
Hsp92II CATG 7 cut(s) 314, 779, 823, 838, 869, 1036, 1096
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 3 cut(s) 697, 778, 946
LguI GCTCTTC 1 cut(s) 351
LmnI GCTCC 1 cut(s) 499
LpnPI CCDG 8 cut(s) 13, 66, 87, 213, 240, 510, 905, 1000
Lsp1109I GCAGC 6 cut(s) 37, 652, 778, 796, 856, 976
LweI GCATC 6 cut(s) 120, 753, 828, 1016, 1086, 1094
MaeI CTAG 3 cut(s) 150, 336, 950
MaeII ACGT 2 cut(s) 213, 258
MaeIII GTNAC 1 cut(s) 157
MalI GATC 3 cut(s) 699, 780, 948
MboI GATC 3 cut(s) 697, 778, 946
MboII GAAGA 7 cut(s) 190, 368, 461, 482, 685, 719, 899
MhlI GDGCHC 1 cut(s) 775
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 6 cut(s) 173, 207, 273, 290, 795, 1067
MluNI TGGCCA 1 cut(s) 296
MlyI GAGTC 2 cut(s) 821, 1004
MmeI TCCRAC 2 cut(s) 52, 1113
MnlI CCTC 9 cut(s) 428, 513, 570, 673, 935, 954, 1018, 1029, 1070
Mox20I TGGCCA 1 cut(s) 296
Mph1103I ATGCAT 1 cut(s) 287
MroXI GAANNNNTTC 1 cut(s) 588
MscI TGGCCA 1 cut(s) 296
MseI TTAA 4 cut(s) 210, 288, 789, 1144
MslI CAYNNNNRTG 1 cut(s) 753
Msp20I TGGCCA 1 cut(s) 296
MspA1I CMGCKG 3 cut(s) 595, 967, 1001
MspI CCGG 1 cut(s) 497
MspR9I CCNGG 2 cut(s) 228, 497
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 5 cut(s) 117, 492, 604, 766, 902
NciI CCSGG 1 cut(s) 497
NdeII GATC 3 cut(s) 697, 778, 946
NlaIII CATG 7 cut(s) 314, 779, 823, 838, 869, 1036, 1096
NlaIV GGNNCC 1 cut(s) 501
NmuCI GTSAC 1 cut(s) 157
NsiI ATGCAT 1 cut(s) 287
NspI RCATGY 1 cut(s) 869
PciSI GCTCTTC 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 588
PfeI GAWTC 2 cut(s) 306, 325
PleI GAGTC 2 cut(s) 820, 1003
PpsI GAGTC 2 cut(s) 820, 1003
PsiI TTATAA 1 cut(s) 1130
Psp1406I AACGTT 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 501
PspPI GGNCC 1 cut(s) 1014
PstI CTGCAG 1 cut(s) 786
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 1 cut(s) 1057
RsaNI GTAC 1 cut(s) 1056
RseI CAYNNNNRTG 1 cut(s) 753
SapI GCTCTTC 1 cut(s) 351
SaqAI TTAA 4 cut(s) 210, 288, 789, 1144
Sau3AI GATC 3 cut(s) 697, 778, 946
Sau96I GGNCC 1 cut(s) 1014
SchI GAGTC 2 cut(s) 821, 1004
ScrFI CCNGG 2 cut(s) 228, 497
SduI GDGCHC 1 cut(s) 775
SfaNI GCATC 6 cut(s) 120, 753, 828, 1016, 1086, 1094
SfcI CTRYAG 1 cut(s) 782
SinI GGWCC 1 cut(s) 1014
SmiMI CAYNNNNRTG 1 cut(s) 753
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
Sse9I AATT 6 cut(s) 173, 207, 273, 290, 795, 1067
SsiI CCGC 5 cut(s) 483, 595, 967, 983, 1099
SspI AATATT 1 cut(s) 405
SspMI CTAG 3 cut(s) 150, 336, 950
StyD4I CCNGG 2 cut(s) 226, 495
StyI CCWWGG 5 cut(s) 115, 297, 373, 517, 561
TaaI ACNGT 3 cut(s) 61, 1060, 1126
TaiI ACGT 2 cut(s) 216, 261
TaqI TCGA 2 cut(s) 14, 582
TaqII GACCGA 1 cut(s) 1031
TasI AATT 6 cut(s) 173, 207, 273, 290, 795, 1067
TauI GCSGC 4 cut(s) 486, 598, 970, 1102
TfiI GAWTC 2 cut(s) 306, 325
Tru1I TTAA 4 cut(s) 210, 288, 789, 1144
Tru9I TTAA 4 cut(s) 210, 288, 789, 1144
TscAI CASTG 2 cut(s) 64, 85
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 6 cut(s) 50, 640, 766, 784, 869, 964
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 6 cut(s) 21, 121, 582, 701, 876, 939
TspGWI ACGGA 2 cut(s) 665, 815
TspRI CASTG 2 cut(s) 64, 85
VneI GTGCAC 1 cut(s) 771
VpaK11BI GGWCC 1 cut(s) 1014
XbaI TCTAGA 1 cut(s) 949
XceI RCATGY 1 cut(s) 869
XmiI GTMKAC 2 cut(s) 219, 1029
XmnI GAANNNNTTC 1 cut(s) 588
XspI CTAG 3 cut(s) 150, 336, 950
Zsp2I ATGCAT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.