Rorug07G0256600
ERF Family

P-loop nucleoside triphosphate hydrolase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000615
Physical Location & Seq
Reverse (-)
23988846 .. 23992116
3271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0256600.1

Sequence Viewer

Length: 1926 bp
ATGGACGCCACCGTCACCCCAGCCCCAATCCCCGACCCGAACGCCGCCCCAGTCGTCTCCTGTTGGAGCAACATCGTGAAGAAGCAGCCGGACCCAAAGCCCCAAAACTCCTCCGACTCCTCCTCAATCGCCGCCGGCAAAGTATTCGTCGAAAGCTGCAAGTCCTCCAAGGGCATAGCCGTCGCCGTCGTCGACGCCAATGCTATCATTCAAGGCGGCGAGACCCTCGCCCACTGGGCCGACAGGTTCGTCTCCGTCCCAGAGGTCATGGACGAGGTCCGTGACCCTGTTTCCCGCCACCGCCTCGCCGTCGTTCCCTTCCAAGTTCAGACCATGGAACCCTCCCCTGAATCCCTCAGTAAAGTTGTTAAGTTTGCGAGGGCTACTGGGGATTTACAGACACTGTCAGATGTGGATGTTAAGCTCATTGCTATGACTTATACATTGGAGGCTCAGATACATGGCACTGAGCACCTCAGGGACTGTCCTCCGCCGGTGCATACTGTCAATGTAAGGAGGTTACCGGAGAAGGACCTGCCCGGGTGGGGAAATAATGTGCCTAATTTGGAAGAGTGGGAAGCGCTGGAGAACGAAGCGGAGGATAAGTTGAACCCCAGTTCAAGGATTCTTCCGTTGAAAGATATAAACTTGAATGTTATTGAGGATTCTGAGAGCCGTTCTGTTGATGGTTCTGTGGTGGAAGTTAGGAGTGATGTTCGTTCTGAGAATCAGGAGGGTGGTGAGGGCATTCAGGGGAGACATAGGAGATATTTTCCAAAGAAGAAAGAGATAAATATTGAAGGGAAGATGGTTTCTGATGGAATTGATGCATCCCAAGGACAAGCTGATGATAATGCTGGGGATTGGATGCCTGCCGTCAGTCGGAGTACACATAGAAGGTTTTTGAGAAGAAAAGCTAGACGTGAGTCTTATGAGGCATCGGCTGATAATGATTCTCAGCAAGATGCTGAGGAAAACACCAGTGGTGACGTACTTGTAGATGCTAGGAGCCAGGATCAAACATCGCCTTCTAAATTTGAAGAGGCATGTTGCAGACCTGTGGAGAGTGAGATGACAGAAGAGAAGAATTGTGGTGATCTTTCCTCAATCCTTGAGCAAACAAGGCATGATGAAGATTCACTAAGGGCTCTTCAAAAAGGGAAGGAGCAGCATGGGGTTGAGGATAATGATTTCAAAGCAGAGGAAAGCACTTCTGATAACGGTGTGAATTTTGCTGTTGAGGGAGATGAGGTTGATGTGATAAATGAAGGTCTGGATCACTTGGAGATCTCAAGTCATAATGATGGTAGTGTTGATACATCAAATGTTAACGATGATAGCAGTGAGCAGAGCTGGATGCTGCGATCCTTGTCTGAGTCTAGTGTAGCATGTATAACAAGTGATTTTGCAATGCAAAATGTTATTCTGCAGATGGGTTTACGGTTGTTGGCACCAGGAGGAATGCAGATCCGCCAGCTGCACAGGTGGATTTTGAAATGCCATGCTTGTAACACTGTGACTGCTGAAATTGGGAGAATATTTTGTCCAAAGTGTGGAAATGGTGGTACCTTGCGCAAGGTAGCAGTTACAGTGGGTGAGAATGGGATTATTCTTGCGGCACGTAAGCCTCGGATCACGTTGCGTGGCACAAGATTTTCACTTCCTTTACCTCAAGGTGGAAGAAATGCCATTACTAAGAACCCTGTTTTACGTGAGGATCAGCTCCCACAAAAGTACCTTCATCCAAAGACAAAAAAGAAAGCAAATAAGCCGGGAGATGACGACCTATTCACTTCCAATGACTTCATCTTCCGCCACCATTCTGATAAAAAGGCTCCTTTGCAGCCTCCTGTAAGGAAGGCATTAGCTGCTTTCAGTGGAAGGAGGAATCCCAACGACAATCATTATTCACATTCCAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AIG1

Protein Analysis

641

Amino Acids

70.73

Weight (kDa)

5.51

Isoelectric Point (pI)

60.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIN_6 PF17146 63 - 149 1.5e-25 PIN domain of ribonuclease
Zn_ribbon_NOB1 PF08772 490 - 560 6.6e-22 Nin one binding (NOB1) Zn-ribbon like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000615)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33970 AT1G33970 AT1G33970 AT1G33970 AT1G33970
fragaria_vesca FvH4_5g32530 FvH4_5g32530
malus_domestica MD15G1403700.v1.1
prunus_persica Prupe.1G549700_v2.0.a1 Prupe.1G549700_v2.0.a1
pyrus_communis pycom15g36100
rosa_chinensis RchiOBHm_Chr7g0226331 RchiOBHm_Chr7g0229101 RchiOBHm_Chr7g0229111 RchiOBHm_Chr7g0229141 RchiOBHm_Chr7g0229161 RchiOBHm_Chr7g0229171 RchiOBHm_Chr7g0229211 RchiOBHm_Chr7g0229241 RchiOBHm_Chr7g0229381 RchiOBHm_Chr7g0229761 RchiOBHm_Chr7g0229911
rosa_laevigata RLG00000000796 RLG00000001464 RLG00000001466 RLG00000001469 RLG00000001523 RLG00000001526 RLG00000001529 RLG00000001530 RLG00000001749 RLG00000001913 RLG00000002114 RLG00000002115 RLG00000023248 RLG00000023250
rosa_multiflora Rmu_co8281135.1_g000001 Rmu_sc0000271.1_g000002 Rmu_sc0000271.1_g000007 Rmu_sc0000271.1_g000014 Rmu_sc0000693.1_g000012 Rmu_sc0000693.1_g000015 Rmu_sc0000693.1_g000023 Rmu_sc0001044.1_g000005 Rmu_sc0006689.1_g000007 Rmu_sc0012733.1_g000005
rosa_roxburghii Rroxscaffold_3G00230540 Rroxscaffold_3G00230590 Rroxscaffold_3G00230600 Rroxscaffold_3G00230700 Rroxscaffold_3G00230710 Rroxscaffold_3G00232980 Rroxscaffold_3G00237610
rosa_rugosa Rorug07G0203800 Rorug07G0203900 Rorug07G0256100 Rorug07G0256400 Rorug07G0256400 Rorug07G0256400 Rorug07G0256500 Rorug07G0256600 Rorug07G0256600 Rorug07G0257100 Rorug07G0257200 Rorug07G0257300 Rorug07G0257400
rosa_samantha Rh7DG401100 Rh7DG401300 Rh7DG401900 Rh7DG402100 Rh7DG402300 Rh7DG402700
rosa_wichuraiana Rw0G001910 Rw0G001930 Rw0G001940 Rw7G033790 Rw7G033810 Rw7G033840 Rw7G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 11, 248
Acc16I TGCGCA 1 cut(s) 1574
Acc36I ACCTGC 1 cut(s) 543
Acc65I GGTACC 1 cut(s) 1565
AccB1I GGYRCC 2 cut(s) 1450, 1565
AccB7I CCANNNNNTGG 1 cut(s) 1553
AccI GTMKAC 1 cut(s) 192
AclWI GGATC 6 cut(s) 1023, 1284, 1359, 1462, 1640, 1725
AcsI RAATTY 2 cut(s) 1034, 1228
AcyI GRCGYC 2 cut(s) 6, 195
AfaI GTAC 4 cut(s) 889, 993, 1567, 1736
AfeI AGCGCT 1 cut(s) 582
AfiI CCNNNNNNNGG 5 cut(s) 545, 621, 882, 1553, 1676
AjiI CACGTC 1 cut(s) 923
AjnI CCWGG 2 cut(s) 1011, 1453
AluBI AGCT 8 cut(s) 156, 424, 845, 917, 1353, 1477, 1723, 1868
AluI AGCT 8 cut(s) 156, 424, 845, 917, 1353, 1477, 1723, 1868
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 4 cut(s) 61, 215, 256, 751
AlwI GGATC 6 cut(s) 1023, 1284, 1359, 1462, 1640, 1725
AlwNI CAGNNNCTG 2 cut(s) 403, 483
Ama87I CYCGRG 1 cut(s) 539
Aor51HI AGCGCT 1 cut(s) 582
AoxI GGCC 1 cut(s) 237
ApeKI GCWGC 7 cut(s) 85, 156, 1168, 1360, 1477, 1843, 1868
ApoI RAATTY 2 cut(s) 1034, 1228
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
Asp718I GGTACC 1 cut(s) 1565
AspLEI GCGC 2 cut(s) 583, 1575
AspS9I GGNCC 4 cut(s) 91, 237, 277, 532
AsuC2I CCSGG 3 cut(s) 540, 541, 1773
AsuHPI GGTGA 5 cut(s) 7, 752, 998, 1106, 1607
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 3 cut(s) 91, 277, 532
AxyI CCTNAGG 1 cut(s) 476
BaeI ACNNNNGTAYC 2 cut(s) 1718, 1751
BanI GGYRCC 2 cut(s) 1450, 1565
BanII GRGCYC 1 cut(s) 1150
Bbv12I GWGCWC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 969
BbvI GCAGC 7 cut(s) 97, 143, 1180, 1347, 1464, 1855, 1855
BccI CCATC 5 cut(s) 680, 802, 812, 1298, 1426
BceAI ACGGC 5 cut(s) 164, 170, 293, 660, 860
BcgI CGANNNNNNTGC 6 cut(s) 127, 161, 163, 182, 197, 216
BciT130I CCWGG 2 cut(s) 1013, 1455
BcnI CCSGG 3 cut(s) 540, 541, 1773
BcoDI GTCTC 4 cut(s) 61, 215, 256, 751
BfaI CTAG 3 cut(s) 918, 1005, 1380
BfmI CTRYAG 1 cut(s) 1427
BfoI RGCGCY 1 cut(s) 584
BfuAI ACCTGC 1 cut(s) 543
BglI GCCNNNNNGGC 1 cut(s) 236
BglII AGATCT 1 cut(s) 1287
Bme1390I CCNGG 5 cut(s) 540, 541, 1013, 1455, 1773
Bme18I GGWCC 3 cut(s) 91, 277, 532
BmeT110I CYCGRG 1 cut(s) 539
BmgBI CACGTC 1 cut(s) 923
BmgT120I GGNCC 4 cut(s) 91, 237, 277, 532
BmiI GGNNCC 6 cut(s) 93, 339, 1010, 1452, 1567, 1836
BmrFI CCNGG 5 cut(s) 540, 541, 1013, 1455, 1773
BmrI ACTGGG 4 cut(s) 44, 244, 396, 609
BmsI GCATC 7 cut(s) 817, 839, 858, 947, 955, 991, 1347
BmuI ACTGGG 4 cut(s) 44, 244, 396, 609
BoxI GACNNNNGTC 1 cut(s) 925
BpmI CTGGAG 1 cut(s) 605
Bpu10I CCTNAGC 1 cut(s) 969
BpuEI CTTGAG 3 cut(s) 1133, 1276, 1656
BpuMI CCSGG 3 cut(s) 540, 541, 1773
BsaAI YACGTR 2 cut(s) 1622, 1712
BsaHI GRCGYC 2 cut(s) 6, 195
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 5 cut(s) 168, 333, 539, 835, 1628
BsaWI WCCGGW 1 cut(s) 523
BsaXI ACNNNNNCTCC 4 cut(s) 590, 620, 1236, 1266
Bsc4I CCNNNNNNNGG 5 cut(s) 545, 621, 882, 1553, 1676
Bse118I RCCGGY 2 cut(s) 134, 493
Bse1I ACTGG 5 cut(s) 50, 239, 391, 615, 981
Bse21I CCTNAGG 1 cut(s) 476
Bse3DI GCAATG 2 cut(s) 426, 1416
BseBI CCWGG 2 cut(s) 1013, 1455
BseDI CCNNGG 5 cut(s) 168, 333, 539, 835, 1628
BseGI GGATG 5 cut(s) 421, 830, 873, 1362, 1741
BseLI CCNNNNNNNGG 5 cut(s) 545, 621, 882, 1553, 1676
BseMI GCAATG 2 cut(s) 426, 1416
BseMII CTCAG 9 cut(s) 370, 459, 467, 490, 660, 714, 960, 971, 1365
BseNI ACTGG 5 cut(s) 50, 239, 391, 615, 981
BseRI GAGGAG 3 cut(s) 100, 109, 112
BseXI GCAGC 7 cut(s) 97, 143, 1180, 1347, 1464, 1855, 1855
BseYI CCCAGC 2 cut(s) 19, 857
BsgI GTGCAG 1 cut(s) 1463
BshFI GGCC 1 cut(s) 239
BshNI GGYRCC 2 cut(s) 1450, 1565
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 6 cut(s) 89, 135, 494, 524, 540, 1772
BslFI GGGAC 2 cut(s) 242, 494
BslI CCNNNNNNNGG 5 cut(s) 545, 621, 882, 1553, 1676
BsmAI GTCTC 4 cut(s) 61, 215, 256, 751
BsmBI CGTCTC 2 cut(s) 61, 256
BsmFI GGGAC 2 cut(s) 242, 494
BsmI GAATGC 2 cut(s) 747, 1467
BsnI GGCC 1 cut(s) 239
Bso31I GGTCTC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 539
Bsp1286I GDGCHC 2 cut(s) 474, 1150
Bsp143I GATC 8 cut(s) 1015, 1096, 1276, 1287, 1364, 1467, 1632, 1717
Bsp19I CCATGG 1 cut(s) 333
BspANI GGCC 1 cut(s) 239
BspCNI CTCAG 9 cut(s) 369, 460, 466, 489, 661, 715, 961, 970, 1366
BspLI GGNNCC 6 cut(s) 93, 339, 1010, 1452, 1567, 1836
BspMAI CTGCAG 1 cut(s) 1431
BspMI ACCTGC 1 cut(s) 543
BspPI GGATC 6 cut(s) 1023, 1284, 1359, 1462, 1640, 1725
BspQI GCTCTTC 1 cut(s) 1155
BspT107I GGYRCC 2 cut(s) 1450, 1565
BspTNI GGTCTC 1 cut(s) 215
BsrDI GCAATG 2 cut(s) 426, 1416
BsrFI RCCGGY 2 cut(s) 134, 493
BsrI ACTGG 5 cut(s) 50, 239, 391, 615, 981
BssAI RCCGGY 2 cut(s) 134, 493
BssECI CCNNGG 5 cut(s) 168, 333, 539, 835, 1628
BssMI GATC 8 cut(s) 1015, 1096, 1276, 1287, 1364, 1467, 1632, 1717
BssNI GRCGYC 2 cut(s) 6, 195
BssT1I CCWWGG 3 cut(s) 168, 333, 835
Bst2UI CCWGG 2 cut(s) 1013, 1455
Bst4CI ACNGT 8 cut(s) 13, 405, 485, 505, 1223, 1443, 1516, 1591
Bst6I CTCTTC 4 cut(s) 564, 1035, 1074, 1155
BstACI GRCGYC 2 cut(s) 6, 195
BstAPI GCANNNNNTGC 1 cut(s) 1868
BstBAI YACGTR 2 cut(s) 1622, 1712
BstC8I GCNNGC 3 cut(s) 136, 873, 1475
BstDSI CCRYGG 1 cut(s) 333
BstEII GGTNACC 1 cut(s) 519
BstF5I GGATG 5 cut(s) 421, 830, 873, 1362, 1741
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 2 cut(s) 583, 1575
BstKTI GATC 8 cut(s) 1018, 1099, 1279, 1290, 1367, 1470, 1635, 1720
BstMAI GTCTC 4 cut(s) 61, 215, 256, 751
BstMBI GATC 8 cut(s) 1015, 1096, 1276, 1287, 1364, 1467, 1632, 1717
BstMWI GCNNNNNNNGC 3 cut(s) 236, 1123, 1868
BstNI CCWGG 2 cut(s) 1013, 1455
BstNSI RCATGY 2 cut(s) 1050, 1392
BstPAI GACNNNNGTC 1 cut(s) 925
BstPI GGTNACC 1 cut(s) 519
BstSCI CCNGG 5 cut(s) 538, 539, 1011, 1453, 1771
BstSFI CTRYAG 1 cut(s) 1427
BstV1I GCAGC 7 cut(s) 97, 143, 1180, 1347, 1464, 1855, 1855
BstX2I RGATCY 2 cut(s) 1287, 1467
BstYI RGATCY 2 cut(s) 1287, 1467
Bsu36I CCTNAGG 1 cut(s) 476
BsuRI GGCC 1 cut(s) 239
BtgI CCRYGG 1 cut(s) 333
BtgZI GCGATG 1 cut(s) 1008
BtrI CACGTC 1 cut(s) 923
BtsCI GGATG 5 cut(s) 421, 830, 873, 1362, 1741
BtsI GCAGTG 1 cut(s) 1348
BtsIMutI CAGTG 8 cut(s) 232, 401, 465, 988, 1348, 1512, 1596, 1882
BveI ACCTGC 1 cut(s) 543
Cac8I GCNNGC 3 cut(s) 136, 873, 1475
CaiI CAGNNNCTG 2 cut(s) 403, 483
CfoI GCGC 2 cut(s) 583, 1575
Cfr10I RCCGGY 2 cut(s) 134, 493
Cfr13I GGNCC 4 cut(s) 91, 237, 277, 532
Cfr9I CCCGGG 1 cut(s) 539
CseI GACGC 2 cut(s) 14, 203
Csp6I GTAC 4 cut(s) 888, 992, 1566, 1735
CviAII CATG 8 cut(s) 268, 334, 461, 1047, 1127, 1172, 1389, 1502
CviQI GTAC 4 cut(s) 888, 992, 1566, 1735
DpnI GATC 8 cut(s) 1017, 1098, 1278, 1289, 1366, 1469, 1634, 1719
DpnII GATC 8 cut(s) 1015, 1096, 1276, 1287, 1364, 1467, 1632, 1717
DrdI GACNNNNNNGTC 2 cut(s) 11, 248
DseDI GACNNNNNNGTC 2 cut(s) 11, 248
Eam1104I CTCTTC 4 cut(s) 564, 1035, 1074, 1155
EarI CTCTTC 4 cut(s) 564, 1035, 1074, 1155
EciI GGCGGA 3 cut(s) 480, 1460, 1802
Eco130I CCWWGG 3 cut(s) 168, 333, 835
Eco24I GRGCYC 1 cut(s) 1150
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 3 cut(s) 91, 277, 532
Eco47III AGCGCT 1 cut(s) 582
Eco81I CCTNAGG 1 cut(s) 476
Eco88I CYCGRG 1 cut(s) 539
Eco91I GGTNACC 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 532
EcoO65I GGTNACC 1 cut(s) 519
EcoRII CCWGG 2 cut(s) 1011, 1453
EcoT14I CCWWGG 3 cut(s) 168, 333, 835
EcoT22I ATGCAT 1 cut(s) 832
EcoT38I GRGCYC 1 cut(s) 1150
ErhI CCWWGG 3 cut(s) 168, 333, 835
Esp3I CGTCTC 2 cut(s) 61, 256
FaeI CATG 8 cut(s) 271, 337, 464, 1050, 1130, 1175, 1392, 1505
FaqI GGGAC 2 cut(s) 242, 494
FatI CATG 8 cut(s) 267, 333, 460, 1046, 1126, 1171, 1388, 1501
FauI CCCGC 1 cut(s) 302
FblI GTMKAC 1 cut(s) 192
FokI GGATG 5 cut(s) 428, 817, 880, 1369, 1728
FriOI GRGCYC 1 cut(s) 1150
FspBI CTAG 3 cut(s) 918, 1005, 1380
FspI TGCGCA 1 cut(s) 1574
GlaI GCGC 2 cut(s) 582, 1574
GsaI CCCAGC 2 cut(s) 23, 861
GsuI CTGGAG 1 cut(s) 605
HaeII RGCGCY 1 cut(s) 584
HaeIII GGCC 1 cut(s) 239
HapII CCGG 6 cut(s) 89, 135, 494, 524, 540, 1772
HgaI GACGC 2 cut(s) 14, 203
HhaI GCGC 2 cut(s) 583, 1575
Hin1I GRCGYC 2 cut(s) 6, 195
Hin1II CATG 8 cut(s) 271, 337, 464, 1050, 1130, 1175, 1392, 1505
Hin6I GCGC 2 cut(s) 581, 1573
HinP1I GCGC 2 cut(s) 581, 1573
HincII GTYRAC 2 cut(s) 193, 1330
HindII GTYRAC 2 cut(s) 193, 1330
HpaI GTTAAC 1 cut(s) 1330
HpaII CCGG 6 cut(s) 89, 135, 494, 524, 540, 1772
HphI GGTGA 5 cut(s) 7, 752, 998, 1106, 1607
Hpy166II GTNNAC 4 cut(s) 193, 890, 1330, 1439
Hpy188III TCNNGA 3 cut(s) 76, 731, 1274
Hpy8I GTNNAC 4 cut(s) 193, 890, 1330, 1439
Hpy99I CGWCG 6 cut(s) 152, 185, 191, 194, 197, 314
HpyCH4III ACNGT 8 cut(s) 13, 405, 485, 505, 1223, 1443, 1516, 1591
HpyCH4IV ACGT 5 cut(s) 922, 990, 1621, 1637, 1711
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 1123, 1868
HpySE526I ACGT 5 cut(s) 922, 990, 1621, 1637, 1711
Hsp92I GRCGYC 2 cut(s) 6, 195
Hsp92II CATG 8 cut(s) 271, 337, 464, 1050, 1130, 1175, 1392, 1505
HspAI GCGC 2 cut(s) 581, 1573
KpnI GGTACC 1 cut(s) 1569
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
KspAI GTTAAC 1 cut(s) 1330
Kzo9I GATC 8 cut(s) 1015, 1096, 1276, 1287, 1364, 1467, 1632, 1717
LguI GCTCTTC 1 cut(s) 1155
LmnI GCTCC 5 cut(s) 66, 1008, 1165, 1728, 1840
Lsp1109I GCAGC 7 cut(s) 97, 143, 1180, 1347, 1464, 1855, 1855
LweI GCATC 7 cut(s) 817, 839, 858, 947, 955, 991, 1347
MaeI CTAG 3 cut(s) 918, 1005, 1380
MaeII ACGT 5 cut(s) 922, 990, 1621, 1637, 1711
MaeIII GTNAC 7 cut(s) 13, 281, 519, 986, 1508, 1516, 1585
MalI GATC 8 cut(s) 1017, 1098, 1278, 1289, 1366, 1469, 1634, 1719
MboI GATC 8 cut(s) 1015, 1096, 1276, 1287, 1364, 1467, 1632, 1717
MflI RGATCY 2 cut(s) 1287, 1467
MhlI GDGCHC 2 cut(s) 474, 1150
MluCI AATT 7 cut(s) 562, 822, 1034, 1087, 1228, 1527, 1921
MlyI GAGTC 3 cut(s) 110, 935, 1385
MmeI TCCRAC 3 cut(s) 44, 138, 863
Mph1103I ATGCAT 1 cut(s) 832
MroNI GCCGGC 1 cut(s) 134
MseI TTAA 4 cut(s) 369, 420, 1329, 1924
MslI CAYNNNNRTG 2 cut(s) 431, 1302
MspA1I CMGCKG 1 cut(s) 1477
MspI CCGG 6 cut(s) 89, 135, 494, 524, 540, 1772
MspR9I CCNGG 5 cut(s) 540, 541, 1013, 1455, 1773
Mva1269I GAATGC 2 cut(s) 747, 1467
MvaI CCWGG 2 cut(s) 1013, 1455
MwoI GCNNNNNNNGC 3 cut(s) 236, 1123, 1868
NaeI GCCGGC 1 cut(s) 136
NciI CCSGG 3 cut(s) 540, 541, 1773
NcoI CCATGG 1 cut(s) 333
NdeII GATC 8 cut(s) 1015, 1096, 1276, 1287, 1364, 1467, 1632, 1717
NgoMIV GCCGGC 1 cut(s) 134
NlaIII CATG 8 cut(s) 271, 337, 464, 1050, 1130, 1175, 1392, 1505
NlaIV GGNNCC 6 cut(s) 93, 339, 1010, 1452, 1567, 1836
NmuCI GTSAC 4 cut(s) 13, 281, 986, 1516
NsbI TGCGCA 1 cut(s) 1574
NsiI ATGCAT 1 cut(s) 832
NspI RCATGY 2 cut(s) 1050, 1392
PciSI GCTCTTC 1 cut(s) 1155
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PctI GAATGC 2 cut(s) 747, 1467
PdiI GCCGGC 1 cut(s) 136
PfeI GAWTC 7 cut(s) 350, 625, 665, 727, 953, 1136, 1888
PflFI GACNNNGTC 2 cut(s) 275, 403
PflMI CCANNNNNTGG 1 cut(s) 1553
PleI GAGTC 3 cut(s) 110, 934, 1384
PpsI GAGTC 3 cut(s) 110, 934, 1384
Ppu21I YACGTR 2 cut(s) 1622, 1712
PpuMI RGGWCCY 1 cut(s) 532
PshAI GACNNNNGTC 1 cut(s) 925
Psp5II RGGWCCY 1 cut(s) 532
Psp6I CCWGG 2 cut(s) 1011, 1453
PspEI GGTNACC 1 cut(s) 519
PspFI CCCAGC 2 cut(s) 19, 857
PspGI CCWGG 2 cut(s) 1011, 1453
PspN4I GGNNCC 6 cut(s) 93, 339, 1010, 1452, 1567, 1836
PspPI GGNCC 4 cut(s) 91, 237, 277, 532
PspPPI RGGWCCY 1 cut(s) 532
PstI CTGCAG 1 cut(s) 1431
PstNI CAGNNNCTG 2 cut(s) 403, 483
PsuI RGATCY 2 cut(s) 1287, 1467
PsyI GACNNNGTC 2 cut(s) 275, 403
PvuII CAGCTG 1 cut(s) 1477
RsaI GTAC 4 cut(s) 889, 993, 1567, 1736
RsaNI GTAC 4 cut(s) 888, 992, 1566, 1735
RseI CAYNNNNRTG 2 cut(s) 431, 1302
SalI GTCGAC 1 cut(s) 191
SapI GCTCTTC 1 cut(s) 1155
SaqAI TTAA 4 cut(s) 369, 420, 1329, 1924
Sau3AI GATC 8 cut(s) 1015, 1096, 1276, 1287, 1364, 1467, 1632, 1717
Sau96I GGNCC 4 cut(s) 91, 237, 277, 532
SchI GAGTC 3 cut(s) 110, 935, 1385
ScrFI CCNGG 5 cut(s) 540, 541, 1013, 1455, 1773
SduI GDGCHC 2 cut(s) 474, 1150
SfaNI GCATC 7 cut(s) 817, 839, 858, 947, 955, 991, 1347
SfcI CTRYAG 1 cut(s) 1427
SgrAI CRCCGGYG 1 cut(s) 493
SgrDI CGTCGACG 1 cut(s) 191
SinI GGWCC 3 cut(s) 91, 277, 532
SmaI CCCGGG 1 cut(s) 541
SmiMI CAYNNNNRTG 2 cut(s) 431, 1302
SmlI CTYRAG 3 cut(s) 1112, 1291, 1671
SmoI CTYRAG 3 cut(s) 1112, 1291, 1671
Sse9I AATT 7 cut(s) 562, 822, 1034, 1087, 1228, 1527, 1921
SspI AATATT 2 cut(s) 796, 1539
SspMI CTAG 3 cut(s) 918, 1005, 1380
StyD4I CCNGG 5 cut(s) 538, 539, 1011, 1453, 1771
StyI CCWWGG 3 cut(s) 168, 333, 835
TaaI ACNGT 8 cut(s) 13, 405, 485, 505, 1223, 1443, 1516, 1591
TaiI ACGT 5 cut(s) 925, 993, 1624, 1640, 1714
TaqI TCGA 2 cut(s) 150, 192
TasI AATT 7 cut(s) 562, 822, 1034, 1087, 1228, 1527, 1921
TatI WGTACW 1 cut(s) 887
TauI GCSGC 4 cut(s) 47, 134, 219, 1619
TfiI GAWTC 7 cut(s) 350, 625, 665, 727, 953, 1136, 1888
Tru1I TTAA 4 cut(s) 369, 420, 1329, 1924
Tru9I TTAA 4 cut(s) 369, 420, 1329, 1924
TscAI CASTG 8 cut(s) 239, 408, 472, 988, 1348, 1519, 1596, 1882
TseFI GTSAC 4 cut(s) 13, 281, 986, 1516
TseI GCWGC 7 cut(s) 85, 156, 1168, 1360, 1477, 1843, 1868
Tsp45I GTSAC 4 cut(s) 13, 281, 986, 1516
TspDTI ATGAA 4 cut(s) 1146, 1281, 1730, 1795
TspGWI ACGGA 3 cut(s) 244, 269, 621
TspMI CCCGGG 1 cut(s) 539
TspRI CASTG 8 cut(s) 239, 408, 472, 988, 1348, 1519, 1596, 1882
Tth111I GACNNNGTC 2 cut(s) 275, 403
Van91I CCANNNNNTGG 1 cut(s) 1553
VpaK11BI GGWCC 3 cut(s) 91, 277, 532
XapI RAATTY 2 cut(s) 1034, 1228
XceI RCATGY 2 cut(s) 1050, 1392
XmaI CCCGGG 1 cut(s) 539
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 3 cut(s) 918, 1005, 1380
Zsp2I ATGCAT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.